RchiOBHm_Chr5g0079511

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000085
Physical Location & Seq
Reverse (-)
85205653 .. 85211698
6046 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35391

Sequence Viewer

Length: 4839 bp
ATGCATTGGCAGAGCAGTTGGGCTTCAAATTGTGGGAGGGAGACAGAAATTGAAAGAATTGCTAGATTGAATAAGGAGATAATGAGAACAGAAAAACTCCTTATAATCTTGGACGATGTTTGGGGGAGAACAGAGTTATCAAAGATAGGGATTCCCAGCTACAAGGAACTTCAAAAGTGCAAGTCCAAAGTCCTACTCACCACAAGGAGATTGAATGTATGCCATACCATGGAGAGCCAAGCAAAAATTAAACTCAGTATCTTATCAGAAGAAGATGCTTGGAACTTGTTTTTGAACAAGGCACGAAGGTCATTTGAATCGACCACTTTTGAGGGTGTAGCGAGGAAGGTAGTTGGAGAATGCAGGGGTCTACCCATTGCATTGATAGCAGTTGCAAGGGCACTTGGAGATAAACATCTGGAGGAATGGCAGACAGCAGCTCAACAATTAGAGAAGTCGCAACTTGCCAACCCTGATCATGACGAAGATGCTTTCAGATGTATAAAATTAAGCTATGATTACTTGAAAGATGAGGACTACAAGTCATGCTTCTTACTGTGTTGCCTATATCCAGAAGACTATGACATCCCAATAGAAGACTTGTTCCGGTACGCAATTGGGGAAGGATTGTTTCGAGATACTGACACAATATATGAAGCCAGAGGAATCGCACATTCAGTGGTCAAGCACCTGAAAGATTCTAGCTTGCTTTTGGAGGGTGAATTCAGTGGACACACTGGTAAATGTGTAAGGATGCATGATGTCGTCCGGGATACAGCCATGAGAATTGCAAAATGTGAAGATGGGCATGGGTTGTTGGTGAAAGCTGGATGTGGTTTAAATCATTGGTCGTGTCGATCACATGAAGGCTACTCTGCAATCTCACTAATGGAGAATAAAATTCGCCGTCTACCCGAAGAGTTGGTAAGTCCAAAACTTCAGATTTTATTACTACAACGGAATACTGATTTAAATGAGATCCCAGAGACCTTTTTCCAAAGTCCCAATGAATTAAGGCTCTTGGATCTTAGCAACACTAGTATTTCCTCTCTACCCCAATCTTTCAGTCTCCTTACCAACCTCCAAGCTTTGTATTTAGATTCTTGCAAGAAATTGATTGACATTTCTATAGTGGGAAAACTGAAGAAGCTTGAAATTCTTAGCATGAGGAAATATTCTGGGACGGAATTGTCGAGAGAAATAGGAAATTTGACCAATCTAAGGATTTTGGAAGTCAGTGGCGCTTTAGTCACAATTCCATCTAAAGTGATATCAAAGTTGCATAAATTAGAGGAATTGTACATGCTGCACTGTGGATTTGAGTACTGGGGGTGTGAAATTGAGGGAAAAGGAGGAGAAACCAATATTGGGTTTGATGAGTTAGCTGGTTTATCAAATTTGAAAATTTTGCAGGTTTGCATATCTGATGCAAAATACATCCCTAAAAATGTTGAGGCCGAACCGAATTGGGTTTATTTTGATATACATATTGGCGGATTCATAAAATCTCTATACAATCGAATAGATCATAATTCAAGATTCTTGCATCTTGAAGGACCAACCATGAGTACCTTACCTGATTGGTTTATCAACGCGGTGACAGAGAAAACAGAGAAGCTAAAGTATGATCGCTGCGAAGGGATCAGTGACATTCTTATGAAATATGACCATGGGAGGTTACATAAACTCAAGCATCTCACAGTAAGTGGTCATTTTATTTCCTGTTTGTACTTGAAAGGGTTGATGAACACAACAAGACGAGTTCAAAATGGACCAGTGTCTGAGAATTTGGAAGAGTTGCACCTGATAGATCTGGTCCGCCTTAATGAGTTGTGTGTTGGTGAGTTACCACCTGGGTCTCTCTCAAATCTCAAGGTACTACATATTTGTTTTTGTCATATGTTGAAGAATGTATCAAAATTGGTACAGAGACTACCAAATTTGGAGAAACTATATTTAAATTATATGGATGAATTGGAATATGTGTTTGGATGCGAAGGGTTAGAGCCGATACAATCAAAACTGAGAGAGATGCATTTGTTGTGTCCAAATTCAGTGAAAAGCATTTGTAGCGGTCCTGTCCCACGTGCAATGTTCCAGAGTCTTAAAAGTTTGACCATTTACCGTTGCGAGCTGCTGCAAAGTCTGTTCGCATCTGATGTAGCCAACTGTCTTGTTCAATTGGAAGACCTTTTAGTAGATAGGTGCCCTTTGTTGGAAAGATTAATTGAAGCAGTGAACAACGAGAAGATTACGGTTCTTTCAAAATTAAAGAATATGGTTTTGAAGAATCTTCCTGTGTTGAATGGTGCAAGTGCTATTGTTGATATTGAGTGTCCTTCATTGGAACGCTTGACTGTGGAGGAATGCCCCCAGTTTTCAACCTCTGCTTCCTACTACTTTGGCAGTAGGAACCCAGTCAAACTCAATGATCCACAACTGCACCGCTTCCTATATGAAAGGCATGTCTCTGTGAAGCAAGGAAACAGTGAAGAACAAGAAGACTGTTCTTTCAACATTGAAGGAATAAATCCTCTTCAGTCCACCACATTTGAGAATGTGGCGAGGAGGGTAGCTGTAGAATGTAAGGGTCTACCGATTGCATTGATAGCTGTTGCAAGGGCACTCGGAGATGAGGACCTGGAGGAATGGGAAAAGGCAGCTGAACGACTAGAGAAGTCGCGATCTCCCAATCCTAACCATGAGGAAGATGCATTCAAATGTATAAGATTAAGCTATGATTACTTGAAACATGAAGACCACAAGTCATGCTTCTTGCTCTGTTGCCTATTCCCAGAAGACCATGAAATCAAAACAGAAGACTTGTTCAGGTATGCGATCGGAAAAAGGTTGTTTCGAGATGCCGAAACAATGGTAGAAGCCAGAGGAATAGTAGTTTCAGTGGTCAAGTACTTGAAAGATTCTAGCTTGCTTATGGATAGCGAGAAAAATGGATGTGTAAAGATGCATGATGTCATCCGGGATACAGCCATTCAAATTGCAGAATCTGAAGATGGGTTTTGGGTGAAAGCTGGCTGTGGTTTAAAGGGTTGGAGGCCACGCGGATTACATGCAGGCTGCACTGCTATTTCACTGATGAGGAACGAAATTCGCAAGCTACCTGAAGAGGAGTTGGTATGTCCACATCTCCAGATTTTATTACTAAACCGGAATGATGATTTAGATGAGATCCCAGAAAAGTTCATCCAAAAGCTGAATGAATTAAGGCTCTTGGATCTTAGCGACACTAGTATTTCCGTGTTACCGCAATCATTCAGTCTCCTTACCAACCTCCAAGCTTTGTATTTAGATTCTCGCAAGAAATTTATTGACATTTCTATAGTGGGAAAACTGAAGAAGCTTGAAATTCTTAGTATGAGAAACTGTCCCGGGAGCGGGGGACTTTCGCGTTCAGGATATGAAAGTCCCGAGGAGAGGGTATTGCCGAGAGAAATAGGACATTTGACCAATCTAAGGATTTTGGATGTCAATCGCTCTTGGGCAAGAGCCTCGGTGAGTGGTGTGGTCACAATTCCATCTAAAGTGATATCAAAGTTGCATAAATTAGAAGAATTGTACATGGTGTACTGTGGATTTGAGGAAGCCAATATTTTTGATGAGTTAGCTGGTTTATCAAATTTGAAAATAGTGCAGCTTGGCATATCTGATGCAAAATACATCCCTAACAATGTTGAGGCCAAGCCAGATTGGGATTACTTTTATATAAGTATCGTCAGCAGACGCGACTCCGCTGCATACAAAACAGGAGATCATAATTCAAGATCCTTGTTTTTTAGGGGAGTAACCATGAGTACTTTACCTGATTGGTTTATCAACGCGGTGACAAAGAAAACAGAGAAGCTAGAGTATGAGTGCTGCAGAGGGATGAGTGACATTCTTATGGAATATGACCATGGGAGGTTACATAAACTCAAGCATCTCACAGTATCTGGTCATTTTCTTGATTCCTATGTGTACATGACAGAGTTGATGAATACAACAAGACGGGTTCAAAAAGGATCAGTGTTTGAGAATTTGGAAGAGTTGCATCTGATATCTCTGATCCACCTTGAGGAGTTGTGTGTTGGTGAGTTACAACCTGGGTCTCTCTCGAATCTCAAGGTATTGCACATGTTTTGTTGTCTTATCTTGAAGAATGTATCAAAATTTGTACAGAGACTACCAAATCTAGAGAAACTAGATTTAAATTGGATGGATGAATTGGAATATGTGTTTGGATGCGAAGGGTTCGAGCCAGAACAATCAAAACTGAGAGAGATGCATTTGTTGGGTCCAAATTTAGTGAAGAAAATTTGTAGTGGTCCTGCCCCACGCGTAATGTTTCAGAGTCTTAAAAGTTTGACCATTTACCGCTGCGAGTTGCTGCGAAGTCTGTTCGCATGTGATGTAGCCCAATGTCTCTTTCAATTGGAAGACCTTCTTGTAGAGCAGTGCCCTTTGTTGGGTACAGTAATTGAAGTAGTGAACAACGAGAAGACGGTTCTACCAAAATTAAAGAACTTGGCTTTGAGGAAACTTCCTATGTTGTATGGTGCAAGTGGTACTGTTGATATTGAGTGTCCTTCATTGGAACACTTGATTGTGGTGGATTGCCCCCAGTTTTCATTTTCAACTTCTTCCAATTACTCTGAATCCCTCGAAAGCATGAACCATGTATCATTTTCAACCTCTGCTTCTGACTACTTTGGCAGCACGAACCCAGTCCAACTCAATGATTTCCAAATGTACCAATTTCTACGTGGCAGGGTAGCAATTGGGCAAAGATGGTTTGATCAATTAATATGTGCGATTCAATTTTTATTCTTCCCAATCTTTTAA

Protein Analysis

1612

Amino Acids

183.64

Weight (kDa)

6.0

Isoelectric Point (pI)

46.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 25 - 102 9.1e-16 NB-ARC domain
LRR_8 PF13855 311 - 369 1.2e-09 Leucine rich repeat
LRR_14 PF23598 320 - 441 2.1e-13 Leucine-rich repeat region
LRR_RPS2 PF23247 597 - 736 7.9e-17 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 677 - 794 4.9e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_8 PF13855 1048 - 1105 2.9e-08 Leucine rich repeat
LRR_14 PF23598 1049 - 1165 3.5e-12 Leucine-rich repeat region
LRR_RPS2 PF23247 1346 - 1485 5.3e-15 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1426 - 1557 2.3e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 104
AasI GACNNNNNNGTC 1 cut(s) 1189
Acc36I ACCTGC 1 cut(s) 1402
AccB1I GGYRCC 1 cut(s) 2205
AccB7I CCANNNNNTGG 1 cut(s) 229
AccBSI CCGCTC 1 cut(s) 3396
AccI GTMKAC 3 cut(s) 370, 910, 2593
AccII CGCG 7 cut(s) 1595, 2683, 3063, 3409, 3744, 3839, 4335
AclWI GGATC 9 cut(s) 973, 1032, 1649, 2426, 3184, 3243, 3777, 4027, 4057
AcuI CTGAAG 6 cut(s) 923, 1163, 2522, 3030, 3144, 3374
AcvI CACGTG 1 cut(s) 2087
AflIII ACRYGT 2 cut(s) 4131, 4333
AhdI GACNNNNNGTC 2 cut(s) 541, 2764
AhlI ACTAGT 2 cut(s) 1037, 3248
AjnI CCWGG 3 cut(s) 1852, 2640, 4099
AjuI GAANNNNNNNTTGG 8 cut(s) 1350, 1382, 1971, 2003, 4218, 4250, 4407, 4439
AlwI GGATC 9 cut(s) 973, 1032, 1649, 2426, 3184, 3243, 3777, 4027, 4057
AlwNI CAGNNNCTG 3 cut(s) 1781, 3008, 3950
Ama87I CYCGRG 2 cut(s) 3389, 3428
AoxI GGCC 3 cut(s) 1455, 3056, 3696
AseI ATTAAT 2 cut(s) 2225, 4799
Asp700I GAANNNNTTC 1 cut(s) 4437
AspLEI GCGC 1 cut(s) 1244
AspS9I GGNCC 7 cut(s) 1556, 1772, 1816, 2075, 2638, 4292, 4322
AsuC2I CCSGG 4 cut(s) 770, 2981, 3390, 3391
AsuHPI GGTGA 9 cut(s) 190, 731, 832, 1609, 1853, 3037, 3526, 3853, 4100
AvaI CYCGRG 2 cut(s) 3389, 3428
AvaII GGWCC 7 cut(s) 1556, 1772, 1816, 2075, 2638, 4292, 4322
BaeGI GKGCMC 4 cut(s) 403, 2210, 2626, 4457
BanI GGYRCC 1 cut(s) 2205
BbrPI CACGTG 1 cut(s) 2087
BbsI GAAGAC 9 cut(s) 582, 603, 2193, 2508, 2763, 2805, 2826, 4440, 4502
BccI CCATC 6 cut(s) 797, 1267, 3008, 3544, 4207, 4779
BceAI ACGGC 1 cut(s) 891
BcgI CGANNNNNNTGC 4 cut(s) 3492, 3526, 3734, 3768
BciT130I CCWGG 3 cut(s) 1854, 2642, 4101
BciVI GTATCC 2 cut(s) 766, 2977
BclI TGATCA 2 cut(s) 475, 4792
BcnI CCSGG 4 cut(s) 770, 2981, 3390, 3391
BcuI ACTAGT 2 cut(s) 1037, 3248
BfaI CTAG 9 cut(s) 63, 702, 1038, 2672, 2925, 3249, 3863, 4190, 4199
BfmI CTRYAG 4 cut(s) 1128, 2577, 3339, 3877
BfoI RGCGCY 1 cut(s) 1245
BfuAI ACCTGC 1 cut(s) 1402
BfuI GTATCC 2 cut(s) 766, 2977
BglII AGATCT 1 cut(s) 1810
BmcAI AGTACT 3 cut(s) 1325, 2912, 3814
Bme1390I CCNGG 7 cut(s) 770, 1854, 2642, 2981, 3390, 3391, 4101
Bme18I GGWCC 7 cut(s) 1556, 1772, 1816, 2075, 2638, 4292, 4322
BmeRI GACNNNNNGTC 2 cut(s) 541, 2764
BmeT110I CYCGRG 2 cut(s) 3389, 3428
BmgT120I GGNCC 7 cut(s) 1556, 1772, 1816, 2075, 2638, 4292, 4322
BmiI GGNNCC 3 cut(s) 2207, 2414, 4293
BmrFI CCNGG 7 cut(s) 770, 1854, 2642, 2981, 3390, 3391, 4101
BmrI ACTGGG 5 cut(s) 1336, 2368, 2411, 4612, 4715
BmuI ACTGGG 5 cut(s) 1336, 2368, 2411, 4612, 4715
BoxI GACNNNNGTC 1 cut(s) 1777
BpiI GAAGAC 9 cut(s) 582, 603, 2193, 2508, 2763, 2805, 2826, 4440, 4502
BpmI CTGGAG 3 cut(s) 440, 2663, 3134
BpuEI CTTGAG 5 cut(s) 1673, 1856, 3917, 4091, 4103
BpuMI CCSGG 4 cut(s) 770, 2981, 3390, 3391
BsaAI YACGTR 2 cut(s) 2087, 4760
BsaBI GATNNNNATC 2 cut(s) 414, 3489
BsaI GGTCTC 3 cut(s) 980, 1863, 4110
BsaJI CCNNGG 8 cut(s) 228, 1669, 1853, 3389, 3429, 3510, 3913, 4100
BsaWI WCCGGW 2 cut(s) 606, 3168
Bse1I ACTGG 7 cut(s) 742, 1331, 1775, 2374, 2417, 4618, 4721
Bse3DI GCAATG 2 cut(s) 375, 2097
Bse8I GATNNNNATC 2 cut(s) 414, 3489
BseBI CCWGG 3 cut(s) 1854, 2642, 4101
BseDI CCNNGG 8 cut(s) 228, 1669, 1853, 3389, 3429, 3510, 3913, 4100
BseJI GATNNNNATC 2 cut(s) 414, 3489
BseMI GCAATG 2 cut(s) 375, 2097
BseMII CTCAG 4 cut(s) 268, 1773, 2015, 4262
BseNI ACTGG 7 cut(s) 742, 1331, 1775, 2374, 2417, 4618, 4721
BseRI GAGGAG 5 cut(s) 1368, 2581, 3143, 3446, 4088
BseSI GKGCMC 4 cut(s) 403, 2210, 2626, 4457
BseYI CCCAGC 1 cut(s) 155
BsgI GTGCAG 4 cut(s) 1292, 2426, 3064, 3671
Bsh1236I CGCG 7 cut(s) 1595, 2683, 3063, 3409, 3744, 3839, 4335
Bsh1285I CGRYCG 1 cut(s) 2841
BshFI GGCC 3 cut(s) 1457, 3058, 3698
BshNI GGYRCC 1 cut(s) 2205
BsiEI CGRYCG 1 cut(s) 2841
BsiHKCI CYCGRG 2 cut(s) 3389, 3428
BsiSI CCGG 5 cut(s) 607, 769, 2980, 3169, 3390
BslFI GGGAC 6 cut(s) 987, 1195, 2066, 3372, 3411, 3414
BsmFI GGGAC 6 cut(s) 987, 1195, 2066, 3372, 3411, 3414
BsmI GAATGC 3 cut(s) 365, 2372, 2714
BsnI GGCC 3 cut(s) 1457, 3058, 3698
Bso31I GGTCTC 3 cut(s) 980, 1863, 4110
BsoBI CYCGRG 2 cut(s) 3389, 3428
Bsp1286I GDGCHC 4 cut(s) 403, 2210, 2626, 4457
Bsp1407I TGTACA 4 cut(s) 1299, 3576, 3975, 4171
Bsp19I CCATGG 3 cut(s) 228, 1669, 3913
Bsp68I TCGCGA 1 cut(s) 2683
BspANI GGCC 3 cut(s) 1457, 3058, 3698
BspCNI CTCAG 4 cut(s) 267, 1774, 2016, 4263
BspFNI CGCG 7 cut(s) 1595, 2683, 3063, 3409, 3744, 3839, 4335
BspHI TCATGA 1 cut(s) 478
BspLI GGNNCC 3 cut(s) 2207, 2414, 4293
BspMAI CTGCAG 1 cut(s) 3881
BspMI ACCTGC 1 cut(s) 1402
BspPI GGATC 9 cut(s) 973, 1032, 1649, 2426, 3184, 3243, 3777, 4027, 4057
BspT107I GGYRCC 1 cut(s) 2205
BspTNI GGTCTC 3 cut(s) 980, 1863, 4110
BsrBI CCGCTC 1 cut(s) 3396
BsrDI GCAATG 2 cut(s) 375, 2097
BsrGI TGTACA 4 cut(s) 1299, 3576, 3975, 4171
BsrI ACTGG 7 cut(s) 742, 1331, 1775, 2374, 2417, 4618, 4721
BssECI CCNNGG 8 cut(s) 228, 1669, 1853, 3389, 3429, 3510, 3913, 4100
BssT1I CCWWGG 3 cut(s) 228, 1669, 3913
Bst2UI CCWGG 3 cut(s) 1854, 2642, 4101
Bst6I CTCTTC 5 cut(s) 912, 1788, 2541, 3120, 4035
BstAUI TGTACA 4 cut(s) 1299, 3576, 3975, 4171
BstBAI YACGTR 2 cut(s) 2087, 4760
BstC8I GCNNGC 6 cut(s) 707, 2132, 2930, 3034, 3076, 3116
BstDSI CCRYGG 3 cut(s) 228, 1669, 3913
BstFNI CGCG 7 cut(s) 1595, 2683, 3063, 3409, 3744, 3839, 4335
BstH2I RGCGCY 1 cut(s) 1245
BstHHI GCGC 1 cut(s) 1244
BstMCI CGRYCG 1 cut(s) 2841
BstMWI GCNNNNNNNGC 3 cut(s) 386, 2070, 2609
BstNI CCWGG 3 cut(s) 1854, 2642, 4101
BstNSI RCATGY 5 cut(s) 1306, 2468, 3074, 4135, 4404
BstPAI GACNNNNGTC 1 cut(s) 1777
BstSCI CCNGG 7 cut(s) 768, 1852, 2640, 2979, 3388, 3389, 4099
BstSFI CTRYAG 4 cut(s) 1128, 2577, 3339, 3877
BstSLI GKGCMC 4 cut(s) 403, 2210, 2626, 4457
BstUI CGCG 7 cut(s) 1595, 2683, 3063, 3409, 3744, 3839, 4335
BstV2I GAAGAC 9 cut(s) 582, 603, 2193, 2508, 2763, 2805, 2826, 4440, 4502
BstX2I RGATCY 6 cut(s) 978, 1024, 1810, 3189, 3235, 3782
BstYI RGATCY 6 cut(s) 978, 1024, 1810, 3189, 3235, 3782
BsuI GTATCC 2 cut(s) 766, 2977
BsuRI GGCC 3 cut(s) 1457, 3058, 3698
BtgI CCRYGG 3 cut(s) 228, 1669, 3913
BtsI GCAGTG 3 cut(s) 2241, 3081, 4457
BtuMI TCGCGA 1 cut(s) 2683
BveI ACCTGC 1 cut(s) 1402
Cac8I GCNNGC 6 cut(s) 707, 2132, 2930, 3034, 3076, 3116
CaiI CAGNNNCTG 3 cut(s) 1781, 3008, 3950
CciI TCATGA 1 cut(s) 478
CfoI GCGC 1 cut(s) 1244
Cfr13I GGNCC 7 cut(s) 1556, 1772, 1816, 2075, 2638, 4292, 4322
Cfr9I CCCGGG 1 cut(s) 3389
CseI GACGC 1 cut(s) 3750
CspCI CAANNNNNGTGG 2 cut(s) 2533, 2568
DraI TTTAAA 5 cut(s) 840, 972, 1959, 3045, 4206
DrdI GACNNNNNNGTC 1 cut(s) 1189
DriI GACNNNNNGTC 2 cut(s) 541, 2764
DseDI GACNNNNNNGTC 1 cut(s) 1189
Eam1104I CTCTTC 5 cut(s) 912, 1788, 2541, 3120, 4035
Eam1105I GACNNNNNGTC 2 cut(s) 541, 2764
EarI CTCTTC 5 cut(s) 912, 1788, 2541, 3120, 4035
EciI GGCGGA 2 cut(s) 1509, 1808
Eco130I CCWWGG 3 cut(s) 228, 1669, 3913
Eco31I GGTCTC 3 cut(s) 980, 1863, 4110
Eco32I GATATC 3 cut(s) 1272, 3549, 4056
Eco47I GGWCC 7 cut(s) 1556, 1772, 1816, 2075, 2638, 4292, 4322
Eco57I CTGAAG 6 cut(s) 923, 1163, 2522, 3030, 3144, 3374
Eco72I CACGTG 1 cut(s) 2087
Eco88I CYCGRG 2 cut(s) 3389, 3428
EcoO109I RGGNCCY 1 cut(s) 2638
EcoRI GAATTC 1 cut(s) 722
EcoRII CCWGG 3 cut(s) 1852, 2640, 4099
EcoRV GATATC 3 cut(s) 1272, 3549, 4056
EcoT14I CCWWGG 3 cut(s) 228, 1669, 3913
EcoT22I ATGCAT 6 cut(s) 6, 759, 2037, 2716, 2970, 4284
ErhI CCWWGG 3 cut(s) 228, 1669, 3913
FalI AAGNNNNNCTT 6 cut(s) 533, 565, 2756, 2788, 4425, 4457
FaqI GGGAC 6 cut(s) 987, 1195, 2066, 3372, 3411, 3414
FauI CCCGC 1 cut(s) 3389
FauNDI CATATG 1 cut(s) 1899
FbaI TGATCA 2 cut(s) 475, 4792
FblI GTMKAC 3 cut(s) 370, 910, 2593
FspBI CTAG 9 cut(s) 63, 702, 1038, 2672, 2925, 3249, 3863, 4190, 4199
GlaI GCGC 1 cut(s) 1243
GsaI CCCAGC 1 cut(s) 159
GsuI CTGGAG 3 cut(s) 440, 2663, 3134
HaeII RGCGCY 1 cut(s) 1245
HaeIII GGCC 3 cut(s) 1457, 3058, 3698
HapII CCGG 5 cut(s) 607, 769, 2980, 3169, 3390
HgaI GACGC 1 cut(s) 3750
HhaI GCGC 1 cut(s) 1244
Hin6I GCGC 1 cut(s) 1242
HinP1I GCGC 1 cut(s) 1242
HindIII AAGCTT 4 cut(s) 1086, 1148, 3297, 3359
HpaII CCGG 5 cut(s) 607, 769, 2980, 3169, 3390
HphI GGTGA 9 cut(s) 190, 731, 832, 1609, 1853, 3037, 3526, 3853, 4100
HpyCH4IV ACGT 2 cut(s) 2086, 4759
HpyF10VI GCNNNNNNNGC 3 cut(s) 386, 2070, 2609
HpySE526I ACGT 2 cut(s) 2086, 4759
HspAI GCGC 1 cut(s) 1242
Ksp22I TGATCA 2 cut(s) 475, 4792
LmnI GCTCC 1 cut(s) 3393
MaeI CTAG 9 cut(s) 63, 702, 1038, 2672, 2925, 3249, 3863, 4190, 4199
MaeII ACGT 2 cut(s) 2086, 4759
MbiI CCGCTC 1 cut(s) 3396
MfeI CAATTG 4 cut(s) 615, 2180, 4427, 4773
MflI RGATCY 6 cut(s) 978, 1024, 1810, 3189, 3235, 3782
MhlI GDGCHC 4 cut(s) 403, 2210, 2626, 4457
MluI ACGCGT 1 cut(s) 4333
MlyI GAGTC 3 cut(s) 2110, 3740, 4357
MmeI TCCRAC 4 cut(s) 334, 2196, 3032, 4750
Mph1103I ATGCAT 6 cut(s) 6, 759, 2037, 2716, 2970, 4284
MroXI GAANNNNTTC 1 cut(s) 4437
MslI CAYNNNNRTG 3 cut(s) 1655, 2719, 3899
MspA1I CMGCKG 3 cut(s) 2663, 3752, 4374
MspI CCGG 5 cut(s) 607, 769, 2980, 3169, 3390
MspR9I CCNGG 7 cut(s) 770, 1854, 2642, 2981, 3390, 3391, 4101
MunI CAATTG 4 cut(s) 615, 2180, 4427, 4773
Mva1269I GAATGC 3 cut(s) 365, 2372, 2714
MvaI CCWGG 3 cut(s) 1854, 2642, 4101
MvnI CGCG 7 cut(s) 1595, 2683, 3063, 3409, 3744, 3839, 4335
MwoI GCNNNNNNNGC 3 cut(s) 386, 2070, 2609
NciI CCSGG 4 cut(s) 770, 2981, 3390, 3391
NcoI CCATGG 3 cut(s) 228, 1669, 3913
NdeI CATATG 1 cut(s) 1899
NlaIV GGNNCC 3 cut(s) 2207, 2414, 4293
NmeAIII GCCGAG 1 cut(s) 3471
NmuCI GTSAC 6 cut(s) 1249, 1597, 1646, 3526, 3841, 3890
NruI TCGCGA 1 cut(s) 2683
NsiI ATGCAT 6 cut(s) 6, 759, 2037, 2716, 2970, 4284
NspI RCATGY 5 cut(s) 1306, 2468, 3074, 4135, 4404
PagI TCATGA 1 cut(s) 478
PciI ACATGT 1 cut(s) 4131
PcsI WCGNNNNNNNCGW 1 cut(s) 1190
PctI GAATGC 3 cut(s) 365, 2372, 2714
PdmI GAANNNNTTC 1 cut(s) 4437
PflMI CCANNNNNTGG 1 cut(s) 229
PfoI TCCNGGA 2 cut(s) 768, 2979
Ple19I CGATCG 1 cut(s) 2841
PleI GAGTC 3 cut(s) 2109, 3740, 4356
PmaCI CACGTG 1 cut(s) 2087
PmlI CACGTG 1 cut(s) 2087
PpsI GAGTC 3 cut(s) 2109, 3740, 4356
Ppu21I YACGTR 2 cut(s) 2087, 4760
PpuMI RGGWCCY 1 cut(s) 2638
PscI ACATGT 1 cut(s) 4131
PshAI GACNNNNGTC 1 cut(s) 1777
PshBI ATTAAT 2 cut(s) 2225, 4799
PsiI TTATAA 1 cut(s) 104
Psp5II RGGWCCY 1 cut(s) 2638
Psp6I CCWGG 3 cut(s) 1852, 2640, 4099
PspCI CACGTG 1 cut(s) 2087
PspFI CCCAGC 1 cut(s) 155
PspGI CCWGG 3 cut(s) 1852, 2640, 4099
PspN4I GGNNCC 3 cut(s) 2207, 2414, 4293
PspPI GGNCC 7 cut(s) 1556, 1772, 1816, 2075, 2638, 4292, 4322
PspPPI RGGWCCY 1 cut(s) 2638
PstI CTGCAG 1 cut(s) 3881
PstNI CAGNNNCTG 3 cut(s) 1781, 3008, 3950
PsuI RGATCY 6 cut(s) 978, 1024, 1810, 3189, 3235, 3782
PvuI CGATCG 1 cut(s) 2841
PvuII CAGCTG 1 cut(s) 2663
RruI TCGCGA 1 cut(s) 2683
RseI CAYNNNNRTG 3 cut(s) 1655, 2719, 3899
Sau96I GGNCC 7 cut(s) 1556, 1772, 1816, 2075, 2638, 4292, 4322
ScaI AGTACT 3 cut(s) 1325, 2912, 3814
SchI GAGTC 3 cut(s) 2110, 3740, 4357
ScrFI CCNGG 7 cut(s) 770, 1854, 2642, 2981, 3390, 3391, 4101
SduI GDGCHC 4 cut(s) 403, 2210, 2626, 4457
SfcI CTRYAG 4 cut(s) 1128, 2577, 3339, 3877
SinI GGWCC 7 cut(s) 1556, 1772, 1816, 2075, 2638, 4292, 4322
SmaI CCCGGG 1 cut(s) 3391
SmiI ATTTAAAT 3 cut(s) 972, 1959, 4206
SmiMI CAYNNNNRTG 3 cut(s) 1655, 2719, 3899
SmlI CTYRAG 5 cut(s) 1688, 1871, 3932, 4070, 4118
SmoI CTYRAG 5 cut(s) 1688, 1871, 3932, 4070, 4118
SpeI ACTAGT 2 cut(s) 1037, 3248
SspI AATATT 3 cut(s) 1175, 1366, 3610
SspMI CTAG 9 cut(s) 63, 702, 1038, 2672, 2925, 3249, 3863, 4190, 4199
StyD4I CCNGG 7 cut(s) 768, 1852, 2640, 2979, 3388, 3389, 4099
StyI CCWWGG 3 cut(s) 228, 1669, 3913
SwaI ATTTAAAT 3 cut(s) 972, 1959, 4206
TaiI ACGT 2 cut(s) 2089, 4762
TaqI TCGA 9 cut(s) 320, 634, 856, 1193, 1519, 2857, 4112, 4251, 4659
TatI WGTACW 9 cut(s) 1299, 1323, 1728, 2910, 3576, 3585, 3812, 3975, 4171
TseFI GTSAC 6 cut(s) 1249, 1597, 1646, 3526, 3841, 3890
Tsp45I GTSAC 6 cut(s) 1249, 1597, 1646, 3526, 3841, 3890
TspGWI ACGGA 3 cut(s) 973, 1199, 3247
TspMI CCCGGG 1 cut(s) 3389
Van91I CCANNNNNTGG 1 cut(s) 229
VpaK11BI GGWCC 7 cut(s) 1556, 1772, 1816, 2075, 2638, 4292, 4322
VspI ATTAAT 2 cut(s) 2225, 4799
XbaI TCTAGA 1 cut(s) 4189
XceI RCATGY 5 cut(s) 1306, 2468, 3074, 4135, 4404
XcmI CCANNNNNNNNNTGG 1 cut(s) 4757
XmaI CCCGGG 1 cut(s) 3389
XmiI GTMKAC 3 cut(s) 370, 910, 2593
XmnI GAANNNNTTC 1 cut(s) 4437
XspI CTAG 9 cut(s) 63, 702, 1038, 2672, 2925, 3249, 3863, 4190, 4199
ZrmI AGTACT 3 cut(s) 1325, 2912, 3814
Zsp2I ATGCAT 6 cut(s) 6, 759, 2037, 2716, 2970, 4284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.