Rw5G048470

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000085
Physical Location & Seq
Reverse (-)
84270299 .. 84280314
10016 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G048470.1

Sequence Viewer

Length: 4008 bp
ATGTCCTGGTTCTCTCCAAGCTCTGATGCATCTCTTCCTTCATCAGCTGAATCATTATCATCATCAGCTGAATTATCATCATCAGCTGTTGAGTGGGAGTATGACGTGACTGACAATGATCTTGTCCAAGCCATTGTGGAATTTGTGTACAGTAAACTACAACCTGTCGTGGTATCCCAGAGCCCTGTCTTGACAAAAATTGAAGGCACATTGGCGGGGAAGCCTAAGCTTATTGTCACTAATTGGAAAAAGGGTCATCTTCTCGGCAGCGGCTCCTTCGGGTCTGTGTATGAAGGAATTACTGATGACGGATGCTTCTTTGCTGTCAAGGAAGTTTCCTTGCTTGATCAAGGAAGCCTGGGGAGGCAAAGAGTTTCTCTACTTGAACAGGAGATTGCTCTTCTGAGTCAATGTGAACATGAGAACATAGTTCAGTATTATGGCACACAAAAGGATGAAGCAAAGCTCTATATCTTTATGGAACTTGTAACCAAAGGCTCCCTTCGAAAGCCATATCAAACGTACCATCTTACAGATTCGCATGTCTCTGTATACACTAGACAGATCTTGCAAGGTCTGAAGTATCTTCACAACCGAAGAGTGATTCACAGGGACATTAAATGTGCAAATCTTTTGGTGCATGCTAATGGATCTGTGAAGCTTGCAGACTTTGGATTGGCAAAGACTATCAAAATGAATGACTTCAAATCTTACCAAGGAACTGCATTCTGGATGGCACCTGAGGTTGTTAATCGTAAGAGTCAAGGTTATGGCTTTTCAGCTGATATATGGAGTCTTGGATGTACTGTGTTGGAGATGTTAACTGGGATGGTTCCATACTCTAATCTCGAATGGATCCAGGCATTATGGAAAATTGGAAAAGGGGAGCCCCCTACGGTTCCTGATTCTCTTTCAAGAGAGGCACAAGATTTCATCCGTCTATGCTTACGAGTTAAGCCAGATAATCGTCCCACTGCTGCTCAGCTTTTAAAACATCCATTTGTAAATAAGCCCCTTCCTCGCACGTCTTCTGGATTGGTATCTCCTTACAATCACCACGGGCACCGTTATGCCTCGATCCCTCAAAAAAATGCTGCATCTCTTCCTTCATCAGTTGAATCGTCATCATCATCATCATCATCATCATCATCAGCTCGTCCGTGGATCTATGATGTGTTTTTGAGTTTCAGGGGTGAAGATACTCGAAGGGGTTTTACATTCCATTTATACGATGAGCTGCAAAAGAGAGGAATCACAACATTCATGGATGATCAAGATCTTCAAGTAGGGGATGATGTTTCTCCCACTCTCCTAAAGGCAATTGAAGGATCAAGGTTAGCAATTGTTGTTCTGTCCGAAAATTATGCTTCTTCTGCTTTTTGTTTGGATGAACTTACAAAGATTTGTGAGTTCATGGAGGGAATTCTTCCAGTTTTTTATAAGGTCGAGCCTAGTGATGTAAGAAATCAAAAGGGGAGTTTTCAAGATGCTTTGGCCAAGCATGAAAGCTCTGGGCGATACGGATCAGAGAAGGTGAAGCAGTGGAGAGATGCTTTAACAATAGTGGCCAATTTGTCTGGGTGGGATACACGGGATTACGACACTGACAATGATCTTGTCCAAGCCATTGTGGAATTTTTGTACAGTAAACTACAACCTTTTGTGGTATCCCAGAGCCCTGTCTTGACAAAAATTGAAGGCACATTGGCGGGGAAGCCTAAGCTTACTGTCACTAGTTGGGAAAAGCTTGATCGTCTCAGCAGCGGCTCTTGTGGGTCTGTGTATGAAGGAATTACTGAGATTGAAAGACAACATATCAAAGGCATTGCGGAATCTGTGTTCAGTACAGCACAACCTATTGAAAGTGAGTTTACAATGCCCATGGGAGATTTTCAAGCATTTGAAGCAACAAGACAGGCCATGGATAAGGTTATGAAGGCACTAAAAGCTGATGAGGTCACAGCCATTGGGGTCTACGGAATGGGAGGAGTTGGAAAAACAACCATGGTTGAACATGTTAGTGCAGAAGTTCGGGATAATGGGATGTTTCATTATGTGATTTCGGCTGTTGTATCCCAAAACCCCGACTTGAGAAAAATTCAAGGCAAATTGGCAGATATGTTGGGCTTTATATTAGAGGAGGAGACAGAAATTGGAAGAGCTCGTAGATTGATGCAGAGGATAAGGAGAGGAAATAGGATCCTCATAATCTTGGACAACATTTGGGAGAGAACAGATTTGTTAAACATAGGAATTCCCAGCAACTACGAGCTTCAAAGATGCAATTCCAAAGTCCTACTCATCACAAGGACACAGAATGTCTGTCATGCCATGGAGTGCCAAGCAAACATTTCTCTTGATGTCTTATCAGAAAAAGATTCTTGGAACTTATTTGTGAACAACGCAAGAATGTTGTTTGAATCGACCAATTTTTGCGATGTTGCAAAGAAGGTAGCTAGATCATGTGCTGGTCTTCCAGTTGCGTTGATAGCAGTTGCGAAGGCACTTGTTGATAAGTGTTTGGATGACTGGAAGGAAGCAGCTCAACGATTAAGGGCTTCTCAAACTGCCAACCCTGAAGATGGGAAAGTTGTGTTCAATTGTATAAAATTAAGCTATGATTACTTAGAATCTGATGATGCCAGGTCATGCTTCTTGCTTTGTTGCCTATTCCCAGAAGACTTTGATATCCAAATTGAAGACTTGCTAAAATATGGGATGGGGAAGGGATTGTTTCAAGATTCCAGCACAATGCATGATGCCAGAGCTAGTGCATATTCAGTGGTCAAGTACCTTCAAGATTGTGGCTTACTTTTGAAATCTGAACGGGACGGATGTGTAAAGATGCATGATGTCATTCGGGATGTGGCCATACGGATTTCGTCATCTGAGGATGGCCCACTGTTTTTTGTACAAGCTGATTGTCAGTTAAAGGAATGGCCAAGTATTAGTGCACATGACGGCTACTCTGCTATCTCACTAATTAGGAATAAAATTTGCAAGCTTCCTGAAGAGTTGGTATGTCCGAAACTCCTTATTTTGTTTCTGCAAAGTAATGTTTGGTTGAATGTGATCCCAGAATGTTTCTTTCAAAGTCTGAATGTGTTGAGGGTATTAGATCTAAGCCAAACCAGAATTTCTTTACTACCCACATCATTCAATATCTTGATCAACCTCCAGACCTTGTATCTAGATGGTTGCTTGTCAACCATAGATAACATATCCATACTCGGAAAATTGAAAAAACTTGAGATTCTTAGTATGAAAGAACTTCCTCTTGTGGAACTTCCAAAAGAAATTGGAGATTTGAACCATCTAAGGATGTTAGACATCACTGGTGATTATGTTGATAATGTTCCATCTGAAGTGATATGCAACCTTACTTCGTTAGAGGAACTATACATGCAATGTAATTTTGCAGACTGGAGGAGTATAGTTGAGGGAGCAGGAGAAGAAATTATTGTTGCGTTTGATGAGTTAACTGGCTTGTCATATCTAAACGTTTTGAAGGTCTTCATATCTGATGCTAAATGTTTGCCTAGAGATGTGGAGGTTGAGCCAAATTGGGTAAAGTTTGATATTTGTATCAGCAGAGACCCATCAATCAGAAAAGGGAGGGACCATGGGGAAATTCGTAAATATACTGTGCCGGCACCTGGGATCGGGAATACTGAAATCCCACCGGAGGATGGCTTCACTTGGCGAAAATACGGCCAGAAGGAGATAATGGGCTGCAGGTTCCCAAGGGGCTACTATAGGTGTACCCACCAAAAGTTATACAATTGCCCAGCCAAGAAGCAAGTTCAGAGGCTTGACAATGATCCCTTAACATTTGAAGTAATATACAGAGGTGACCATACGTGTCATATGTCGTCCACTGCACCCTCAAAATATGACCCAAGTTCATGTGGCAACCGCTCAACCTTAGGAACATGGCTCTCCATGGACTTCAACGCGAGAGGCGGAGGAAGCAGCAGTGGTGGTGGCCGCAGTGATGGTATGTACTAG

Protein Analysis

1335

Amino Acids

149.34

Weight (kDa)

5.76

Isoelectric Point (pI)

45.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 83 - 335 1.5e-68 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 85 - 331 2.2e-48 Protein tyrosine and serine/threonine kinase
TIR PF01582 390 - 557 9.6e-47 TIR domain
TIR_2 PF13676 392 - 484 8e-14 TIR domain
NB-ARC PF00931 639 - 802 1.3e-27 NB-ARC domain
LRR_14 PF23598 1060 - 1137 1.2e-10 Leucine-rich repeat region
WRKY PF03106 1229 - 1289 1.2e-24 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1440
Acc36I ACCTGC 1 cut(s) 3726
AccB1I GGYRCC 3 cut(s) 736, 1062, 3652
AccBSI CCGCTC 1 cut(s) 3918
AccI GTMKAC 2 cut(s) 552, 1974
AccII CGCG 1 cut(s) 3956
AciI CCGC 8 cut(s) 215, 270, 1709, 1764, 1829, 3916, 3963, 3988
AclI AACGTT 1 cut(s) 3501
AcoI YGGCCR 6 cut(s) 1494, 1566, 2868, 2939, 3712, 3985
AcsI RAATTY 8 cut(s) 140, 1422, 1634, 2097, 2253, 2994, 3135, 3630
AcuI CTGAAG 4 cut(s) 599, 2598, 3030, 3384
AfaI GTAC 9 cut(s) 149, 524, 805, 1643, 1846, 2792, 2913, 3763, 4004
AfiI CCNNNNNNNGG 5 cut(s) 2582, 3656, 3662, 3685, 3689
AflIII ACRYGT 2 cut(s) 2014, 3860
AhlI ACTAGT 1 cut(s) 1733
AjiI CACGTC 2 cut(s) 106, 1026
AjnI CCWGG 5 cut(s) 5, 357, 858, 2642, 3655
AloI GAACNNNNNNTCC 2 cut(s) 1823, 1855
Alw21I GWGCWC 2 cut(s) 2164, 2956
Alw26I GTCTC 4 cut(s) 550, 1760, 2138, 3588
Alw44I GTGCAC 1 cut(s) 2952
AoxI GGCC 8 cut(s) 1494, 1566, 1917, 2868, 2896, 2939, 3712, 3985
ApaLI GTGCAC 1 cut(s) 2952
ApeKI GCWGC 8 cut(s) 267, 977, 1094, 1237, 1761, 2540, 3732, 3972
ApoI RAATTY 8 cut(s) 140, 1422, 1634, 2097, 2253, 2994, 3135, 3630
Asp700I GAANNNNTTC 2 cut(s) 701, 3512
AspS9I GGNCC 2 cut(s) 2897, 3619
AsuHPI GGTGA 5 cut(s) 1046, 1205, 1546, 3350, 3863
AsuII TTCGAA 1 cut(s) 505
AvaII GGWCC 1 cut(s) 3619
AxyI CCTNAGG 2 cut(s) 741, 3925
BaeGI GKGCMC 2 cut(s) 1065, 2956
BalI TGGCCA 4 cut(s) 1496, 1568, 2870, 2941
BamHI GGATCC 2 cut(s) 855, 2199
BanI GGYRCC 3 cut(s) 736, 1062, 3652
BanII GRGCYC 4 cut(s) 185, 891, 1679, 2164
BarI GAAGNNNNNNTAC 2 cut(s) 1198, 1230
BbsI GAAGAC 5 cut(s) 1020, 2465, 2685, 2706, 3505
Bbv12I GWGCWC 2 cut(s) 2164, 2956
BbvI GCAGC 8 cut(s) 279, 964, 1081, 1224, 1773, 2552, 3719, 3984
BceAI ACGGC 2 cut(s) 2977, 3727
BcgI CGANNNNNNTGC 2 cut(s) 1346, 1380
BciT130I CCWGG 5 cut(s) 7, 359, 860, 2644, 3657
BciVI GTATCC 4 cut(s) 184, 1579, 1678, 2083
BclI TGATCA 3 cut(s) 346, 1270, 3168
BcoDI GTCTC 4 cut(s) 550, 1760, 2138, 3588
BcuI ACTAGT 1 cut(s) 1733
BfaI CTAG 8 cut(s) 558, 1452, 1734, 2457, 2769, 3191, 3540, 4006
BfmI CTRYAG 2 cut(s) 3733, 3754
BfuAI ACCTGC 1 cut(s) 3726
BfuI GTATCC 4 cut(s) 184, 1579, 1678, 2083
BglII AGATCT 3 cut(s) 564, 1276, 3118
BlpI GCTNAGC 1 cut(s) 981
Bme1390I CCNGG 5 cut(s) 7, 359, 860, 2644, 3657
Bme18I GGWCC 1 cut(s) 3619
BmgBI CACGTC 2 cut(s) 106, 1026
BmgT120I GGNCC 2 cut(s) 2897, 3619
BmrFI CCNGG 5 cut(s) 7, 359, 860, 2644, 3657
BmrI ACTGGG 1 cut(s) 834
BmuI ACTGGG 1 cut(s) 834
BpiI GAAGAC 5 cut(s) 1020, 2465, 2685, 2706, 3505
BpmI CTGGAG 2 cut(s) 3161, 3445
Bpu10I CCTNAGC 2 cut(s) 225, 1719
Bpu1102I GCTNAGC 1 cut(s) 981
Bpu14I TTCGAA 1 cut(s) 505
BpuEI CTTGAG 2 cut(s) 2110, 3269
BsaAI YACGTR 1 cut(s) 3861
BsaBI GATNNNNATC 3 cut(s) 1039, 1275, 1522
BsaI GGTCTC 1 cut(s) 3588
BsaWI WCCGGW 1 cut(s) 3682
Bsc4I CCNNNNNNNGG 5 cut(s) 2582, 3656, 3662, 3685, 3689
Bse118I RCCGGY 1 cut(s) 3649
Bse1I ACTGG 7 cut(s) 829, 1430, 2477, 2534, 3340, 3428, 3487
Bse21I CCTNAGG 2 cut(s) 741, 3925
Bse3DI GCAATG 2 cut(s) 1824, 3413
Bse8I GATNNNNATC 3 cut(s) 1039, 1275, 1522
BseBI CCWGG 5 cut(s) 7, 359, 860, 2644, 3657
BseJI GATNNNNATC 3 cut(s) 1039, 1275, 1522
BseLI CCNNNNNNNGG 5 cut(s) 2582, 3656, 3662, 3685, 3689
BseMI GCAATG 2 cut(s) 1824, 3413
BseMII CTCAG 6 cut(s) 395, 732, 995, 1771, 1788, 2880
BseNI ACTGG 7 cut(s) 829, 1430, 2477, 2534, 3340, 3428, 3487
BseRI GAGGAG 4 cut(s) 2001, 2153, 2156, 3442
BseSI GKGCMC 2 cut(s) 1065, 2956
BseXI GCAGC 8 cut(s) 279, 964, 1081, 1224, 1773, 2552, 3719, 3984
BseYI CCCAGC 2 cut(s) 2258, 3787
BsgI GTGCAG 2 cut(s) 2043, 3864
Bsh1236I CGCG 1 cut(s) 3956
BshFI GGCC 8 cut(s) 1496, 1568, 1919, 2870, 2898, 2941, 3714, 3987
BshNI GGYRCC 3 cut(s) 736, 1062, 3652
BsiHKAI GWGCWC 2 cut(s) 2164, 2956
BsiSI CCGG 2 cut(s) 3650, 3683
BslFI GGGAC 4 cut(s) 626, 954, 2843, 3632
BslI CCNNNNNNNGG 5 cut(s) 2582, 3656, 3662, 3685, 3689
BsmAI GTCTC 4 cut(s) 550, 1760, 2138, 3588
BsmBI CGTCTC 1 cut(s) 1760
BsmFI GGGAC 4 cut(s) 626, 954, 2843, 3632
BsmI GAATGC 1 cut(s) 725
BsnI GGCC 8 cut(s) 1496, 1568, 1919, 2870, 2898, 2941, 3714, 3987
Bso31I GGTCTC 1 cut(s) 3588
Bsp119I TTCGAA 1 cut(s) 505
Bsp1286I GDGCHC 6 cut(s) 185, 891, 1065, 1679, 2164, 2956
Bsp1407I TGTACA 3 cut(s) 147, 1641, 2911
Bsp1720I GCTNAGC 1 cut(s) 981
Bsp19I CCATGG 6 cut(s) 1881, 1920, 2004, 2331, 3622, 3942
BspACI CCGC 8 cut(s) 215, 270, 1709, 1764, 1829, 3916, 3963, 3988
BspANI GGCC 8 cut(s) 1496, 1568, 1919, 2870, 2898, 2941, 3714, 3987
BspCNI CTCAG 6 cut(s) 396, 733, 994, 1770, 1789, 2881
BspFNI CGCG 1 cut(s) 3956
BspMAI CTGCAG 1 cut(s) 3737
BspMI ACCTGC 1 cut(s) 3726
BspQI GCTCTTC 2 cut(s) 405, 2152
BspT104I TTCGAA 1 cut(s) 505
BspT107I GGYRCC 3 cut(s) 736, 1062, 3652
BspTNI GGTCTC 1 cut(s) 3588
BsrBI CCGCTC 1 cut(s) 3918
BsrDI GCAATG 2 cut(s) 1824, 3413
BsrFI RCCGGY 1 cut(s) 3649
BsrGI TGTACA 3 cut(s) 147, 1641, 2911
BsrI ACTGG 7 cut(s) 829, 1430, 2477, 2534, 3340, 3428, 3487
BssAI RCCGGY 1 cut(s) 3649
BssNAI GTATAC 1 cut(s) 553
BssT1I CCWWGG 8 cut(s) 715, 1881, 1920, 2004, 2331, 3622, 3743, 3942
Bst1107I GTATAC 1 cut(s) 553
Bst2UI CCWGG 5 cut(s) 7, 359, 860, 2644, 3657
Bst4CI ACNGT 8 cut(s) 152, 808, 898, 1067, 1646, 1729, 2904, 3646
Bst6I CTCTTC 6 cut(s) 39, 405, 592, 1107, 2152, 3006
BstAUI TGTACA 3 cut(s) 147, 1641, 2911
BstBAI YACGTR 1 cut(s) 3861
BstBI TTCGAA 1 cut(s) 505
BstC8I GCNNGC 4 cut(s) 642, 663, 3002, 3651
BstDSI CCRYGG 8 cut(s) 1057, 1160, 1881, 1920, 2004, 2331, 3622, 3942
BstEII GGTNACC 1 cut(s) 3851
BstFNI CGCG 1 cut(s) 3956
BstMAI GTCTC 4 cut(s) 550, 1760, 2138, 3588
BstMWI GCNNNNNNNGC 5 cut(s) 1373, 1904, 1946, 2489, 3969
BstNI CCWGG 5 cut(s) 7, 359, 860, 2644, 3657
BstNSI RCATGY 4 cut(s) 545, 644, 2018, 3406
BstPI GGTNACC 1 cut(s) 3851
BstSCI CCNGG 5 cut(s) 5, 357, 858, 2642, 3655
BstSFI CTRYAG 2 cut(s) 3733, 3754
BstSLI GKGCMC 2 cut(s) 1065, 2956
BstUI CGCG 1 cut(s) 3956
BstV1I GCAGC 8 cut(s) 279, 964, 1081, 1224, 1773, 2552, 3719, 3984
BstV2I GAAGAC 5 cut(s) 1020, 2465, 2685, 2706, 3505
BstX2I RGATCY 7 cut(s) 564, 650, 855, 1164, 1276, 2199, 3118
BstYI RGATCY 7 cut(s) 564, 650, 855, 1164, 1276, 2199, 3118
BstZ17I GTATAC 1 cut(s) 553
Bsu36I CCTNAGG 2 cut(s) 741, 3925
BsuI GTATCC 4 cut(s) 184, 1579, 1678, 2083
BsuRI GGCC 8 cut(s) 1496, 1568, 1919, 2870, 2898, 2941, 3714, 3987
BtgI CCRYGG 8 cut(s) 1057, 1160, 1881, 1920, 2004, 2331, 3622, 3942
BtgZI GCGATG 1 cut(s) 2451
BtrI CACGTC 2 cut(s) 106, 1026
BtsI GCAGTG 5 cut(s) 972, 1547, 3876, 3982, 3997
BtsIMutI CAGTG 9 cut(s) 972, 1547, 1602, 2787, 2900, 3333, 3876, 3982, 3997
BveI ACCTGC 1 cut(s) 3726
Cac8I GCNNGC 4 cut(s) 642, 663, 3002, 3651
Cfr10I RCCGGY 1 cut(s) 3649
Cfr13I GGNCC 2 cut(s) 2897, 3619
Csp6I GTAC 9 cut(s) 148, 523, 804, 1642, 1845, 2791, 2912, 3762, 4003
CviQI GTAC 9 cut(s) 148, 523, 804, 1642, 1845, 2791, 2912, 3762, 4003
DraI TTTAAA 1 cut(s) 990
EaeI YGGCCR 6 cut(s) 1494, 1566, 2868, 2939, 3712, 3985
Eam1104I CTCTTC 6 cut(s) 39, 405, 592, 1107, 2152, 3006
EarI CTCTTC 6 cut(s) 39, 405, 592, 1107, 2152, 3006
EciI GGCGGA 1 cut(s) 3978
Ecl136II GAGCTC 1 cut(s) 2162
Eco130I CCWWGG 8 cut(s) 715, 1881, 1920, 2004, 2331, 3622, 3743, 3942
Eco24I GRGCYC 4 cut(s) 185, 891, 1679, 2164
Eco31I GGTCTC 1 cut(s) 3588
Eco32I GATATC 1 cut(s) 2689
Eco47I GGWCC 1 cut(s) 3619
Eco53kI GAGCTC 1 cut(s) 2162
Eco57I CTGAAG 4 cut(s) 599, 2598, 3030, 3384
Eco81I CCTNAGG 2 cut(s) 741, 3925
Eco91I GGTNACC 1 cut(s) 3851
EcoICRI GAGCTC 1 cut(s) 2162
EcoO65I GGTNACC 1 cut(s) 3851
EcoRI GAATTC 2 cut(s) 1422, 2253
EcoRII CCWGG 5 cut(s) 5, 357, 858, 2642, 3655
EcoRV GATATC 1 cut(s) 2689
EcoT14I CCWWGG 8 cut(s) 715, 1881, 1920, 2004, 2331, 3622, 3743, 3942
EcoT22I ATGCAT 3 cut(s) 31, 2757, 2850
EcoT38I GRGCYC 4 cut(s) 185, 891, 1679, 2164
ErhI CCWWGG 8 cut(s) 715, 1881, 1920, 2004, 2331, 3622, 3743, 3942
Esp3I CGTCTC 1 cut(s) 1760
FalI AAGNNNNNCTT 2 cut(s) 486, 518
FaqI GGGAC 4 cut(s) 626, 954, 2843, 3632
FauI CCCGC 2 cut(s) 208, 1702
FauNDI CATATG 1 cut(s) 3867
FbaI TGATCA 3 cut(s) 346, 1270, 3168
FblI GTMKAC 2 cut(s) 552, 1974
FriOI GRGCYC 4 cut(s) 185, 891, 1679, 2164
FspBI CTAG 8 cut(s) 558, 1452, 1734, 2457, 2769, 3191, 3540, 4006
GsaI CCCAGC 2 cut(s) 2262, 3791
GsuI CTGGAG 2 cut(s) 3161, 3445
HaeIII GGCC 8 cut(s) 1496, 1568, 1919, 2870, 2898, 2941, 3714, 3987
HapII CCGG 2 cut(s) 3650, 3683
HincII GTYRAC 3 cut(s) 822, 3207, 3480
HindII GTYRAC 3 cut(s) 822, 3207, 3480
HindIII AAGCTT 5 cut(s) 227, 659, 1721, 1745, 3002
HpaI GTTAAC 2 cut(s) 822, 3480
HpaII CCGG 2 cut(s) 3650, 3683
HphI GGTGA 5 cut(s) 1046, 1205, 1546, 3350, 3863
HpyCH4III ACNGT 8 cut(s) 152, 808, 898, 1067, 1646, 1729, 2904, 3646
HpyCH4IV ACGT 5 cut(s) 105, 521, 1025, 3501, 3860
HpyF10VI GCNNNNNNNGC 5 cut(s) 1373, 1904, 1946, 2489, 3969
HpySE526I ACGT 5 cut(s) 105, 521, 1025, 3501, 3860
KroI GCCGGC 1 cut(s) 3649
KroNI GCCGGC 1 cut(s) 3651
Ksp22I TGATCA 3 cut(s) 346, 1270, 3168
KspAI GTTAAC 2 cut(s) 822, 3480
LguI GCTCTTC 2 cut(s) 405, 2152
LmnI GCTCC 4 cut(s) 278, 503, 886, 3443
Lsp1109I GCAGC 8 cut(s) 279, 964, 1081, 1224, 1773, 2552, 3719, 3984
MaeI CTAG 8 cut(s) 558, 1452, 1734, 2457, 2769, 3191, 3540, 4006
MaeII ACGT 5 cut(s) 105, 521, 1025, 3501, 3860
MaeIII GTNAC 6 cut(s) 106, 235, 487, 1729, 1957, 3851
MbiI CCGCTC 1 cut(s) 3918
MfeI CAATTG 4 cut(s) 1320, 1341, 2599, 3781
MflI RGATCY 7 cut(s) 564, 650, 855, 1164, 1276, 2199, 3118
MhlI GDGCHC 6 cut(s) 185, 891, 1065, 1679, 2164, 2956
MlsI TGGCCA 4 cut(s) 1496, 1568, 2870, 2941
MluNI TGGCCA 4 cut(s) 1496, 1568, 2870, 2941
MlyI GAGTC 3 cut(s) 415, 769, 802
MmeI TCCRAC 2 cut(s) 792, 1972
Mox20I TGGCCA 4 cut(s) 1496, 1568, 2870, 2941
Mph1103I ATGCAT 3 cut(s) 31, 2757, 2850
MroNI GCCGGC 1 cut(s) 3649
MroXI GAANNNNTTC 2 cut(s) 701, 3512
MscI TGGCCA 4 cut(s) 1496, 1568, 2870, 2941
MslI CAYNNNNRTG 3 cut(s) 645, 1068, 2019
Msp20I TGGCCA 4 cut(s) 1496, 1568, 2870, 2941
MspA1I CMGCKG 6 cut(s) 47, 68, 86, 270, 782, 1764
MspI CCGG 2 cut(s) 3650, 3683
MspR9I CCNGG 5 cut(s) 7, 359, 860, 2644, 3657
MunI CAATTG 4 cut(s) 1320, 1341, 2599, 3781
Mva1269I GAATGC 1 cut(s) 725
MvaI CCWGG 5 cut(s) 7, 359, 860, 2644, 3657
MvnI CGCG 1 cut(s) 3956
MwoI GCNNNNNNNGC 5 cut(s) 1373, 1904, 1946, 2489, 3969
NaeI GCCGGC 1 cut(s) 3651
NcoI CCATGG 6 cut(s) 1881, 1920, 2004, 2331, 3622, 3942
NdeI CATATG 1 cut(s) 3867
NgoMIV GCCGGC 1 cut(s) 3649
NmeAIII GCCGAG 1 cut(s) 243
NmuCI GTSAC 5 cut(s) 106, 235, 1729, 1957, 3851
NsiI ATGCAT 3 cut(s) 31, 2757, 2850
NspI RCATGY 4 cut(s) 545, 644, 2018, 3406
NspV TTCGAA 1 cut(s) 505
PaeI GCATGC 1 cut(s) 644
PciI ACATGT 1 cut(s) 2014
PciSI GCTCTTC 2 cut(s) 405, 2152
PctI GAATGC 1 cut(s) 725
PdiI GCCGGC 1 cut(s) 3651
PdmI GAANNNNTTC 2 cut(s) 701, 3512
PleI GAGTC 3 cut(s) 414, 768, 801
PpsI GAGTC 3 cut(s) 414, 768, 801
Ppu21I YACGTR 1 cut(s) 3861
PscI ACATGT 1 cut(s) 2014
PsiI TTATAA 1 cut(s) 1440
Psp124BI GAGCTC 1 cut(s) 2164
Psp1406I AACGTT 1 cut(s) 3501
Psp6I CCWGG 5 cut(s) 5, 357, 858, 2642, 3655
PspEI GGTNACC 1 cut(s) 3851
PspFI CCCAGC 2 cut(s) 2258, 3787
PspGI CCWGG 5 cut(s) 5, 357, 858, 2642, 3655
PspPI GGNCC 2 cut(s) 2897, 3619
PstI CTGCAG 1 cut(s) 3737
PsuI RGATCY 7 cut(s) 564, 650, 855, 1164, 1276, 2199, 3118
PvuII CAGCTG 4 cut(s) 47, 68, 86, 782
RsaI GTAC 9 cut(s) 149, 524, 805, 1643, 1846, 2792, 2913, 3763, 4004
RsaNI GTAC 9 cut(s) 148, 523, 804, 1642, 1845, 2791, 2912, 3762, 4003
RseI CAYNNNNRTG 3 cut(s) 645, 1068, 2019
SacI GAGCTC 1 cut(s) 2164
SapI GCTCTTC 2 cut(s) 405, 2152
Sau96I GGNCC 2 cut(s) 2897, 3619
SchI GAGTC 3 cut(s) 415, 769, 802
ScrFI CCNGG 5 cut(s) 7, 359, 860, 2644, 3657
SduI GDGCHC 6 cut(s) 185, 891, 1065, 1679, 2164, 2956
SfcI CTRYAG 2 cut(s) 3733, 3754
SfuI TTCGAA 1 cut(s) 505
SinI GGWCC 1 cut(s) 3619
SmiMI CAYNNNNRTG 3 cut(s) 645, 1068, 2019
SmlI CTYRAG 2 cut(s) 2089, 3248
SmoI CTYRAG 2 cut(s) 2089, 3248
SpeI ACTAGT 1 cut(s) 1733
SphI GCATGC 1 cut(s) 644
SsiI CCGC 8 cut(s) 215, 270, 1709, 1764, 1829, 3916, 3963, 3988
SspMI CTAG 8 cut(s) 558, 1452, 1734, 2457, 2769, 3191, 3540, 4006
SstI GAGCTC 1 cut(s) 2164
StyD4I CCNGG 5 cut(s) 5, 357, 858, 2642, 3655
StyI CCWWGG 8 cut(s) 715, 1881, 1920, 2004, 2331, 3622, 3743, 3942
TaaI ACNGT 8 cut(s) 152, 808, 898, 1067, 1646, 1729, 2904, 3646
TaiI ACGT 5 cut(s) 108, 524, 1028, 3504, 3863
TaqI TCGA 6 cut(s) 505, 849, 1076, 1204, 1446, 2423
TatI WGTACW 6 cut(s) 147, 803, 1641, 1844, 2911, 4002
TauI GCSGC 3 cut(s) 273, 1767, 3990
TscAI CASTG 9 cut(s) 979, 1547, 1609, 2787, 2907, 3340, 3883, 3982, 3997
TseFI GTSAC 5 cut(s) 106, 235, 1729, 1957, 3851
TseI GCWGC 8 cut(s) 267, 977, 1094, 1237, 1761, 2540, 3732, 3972
Tsp45I GTSAC 5 cut(s) 106, 235, 1729, 1957, 3851
TspGWI ACGGA 7 cut(s) 324, 926, 1149, 1536, 1992, 2847, 2890
TspRI CASTG 9 cut(s) 979, 1547, 1609, 2787, 2907, 3340, 3883, 3982, 3997
VneI GTGCAC 1 cut(s) 2952
VpaK11BI GGWCC 1 cut(s) 3619
XapI RAATTY 8 cut(s) 140, 1422, 1634, 2097, 2253, 2994, 3135, 3630
XbaI TCTAGA 1 cut(s) 3190
XceI RCATGY 4 cut(s) 545, 644, 2018, 3406
XmiI GTMKAC 2 cut(s) 552, 1974
XmnI GAANNNNTTC 2 cut(s) 701, 3512
XspI CTAG 8 cut(s) 558, 1452, 1734, 2457, 2769, 3191, 3540, 4006
Zsp2I ATGCAT 3 cut(s) 31, 2757, 2850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.