Rw5G048170

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000085
Physical Location & Seq
Reverse (-)
83631840 .. 83636209
4370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G048170.1

Sequence Viewer

Length: 3498 bp
ATGGAATTCTATTACTTCCAGATGGCCATGGCGATTATTGCGGTAATTGTGACATGGATTGGTGTCATAGCTACTGTTAAGTGGATATGGACTCTAGTGAAGCATCGGTTGGGTTACATGGTTGAAAAGCATGAGCCAACATCGCAGGATAATCAAGAGTTCGTCAACATCGATGCCTCCAGACCTCTTCCTTCACCATCAGCTGAATCATCAGCAGCTCGTCGATGGAATTATGATGTGTTCTTGAGTTTCAGGGGTCCTGACACTCGCAAGGGTATTACATTTGAATTATATGATCGACTGCACAGGAGGGGAATTAAAACATTCATGGATGACCAAGATCTTCAAGTAGGGGATACTATTTCTCCCACTCTACTAAAAGCAATTGAAGAATCAAGGTTGGCAATTGTTGTTCTGTCCGAAAAGTATGCTTCTTCTACTTGGTGTTTGGAGGAACTTACAAAGATCTGTGAGTGCATGAAAGACGACAACAGAATTCTTCCACTTTTTTATAATGTCGAGCCTACTGATGTAAGATATCAGAAGAGAAGTTTCGAAGAAGCTTTCACCAAATATGAAAATTCTGGGCGACATAGATCGGAGAAGGTCCAACAATGGAGAGATGCTTTAAACAAAGTGGCTCATTTCTCTGGTTGGGATTCAAAGAAATACAAGACTGAAAGAGAACTCATCGAAGACATTGTGGAATATGTCTGTAGTAAAGTACAACCTATTGCAATCAACATGGGAGATTTTGAAGAATTTGAAGCAACAAGACAAGCCATGAATATGGTTATGAAAGGGCTAGAAGATGACGAGGTCACTGCCATTGGAGTCTACGGAATGGGAGGCGTCGGCAAGACAACCATGGTAAAACATATCAGTGATCAAGCTCGAAAAAATGGAATCTTTCGTCATGTAATTATGGCTACTATATCACAAAGCCCTGACTTGAGGAAAATTCAAGGCCAATTGGCAGATCTGTTGGGCTTCGAATTCATGGAGGAGACTGAAATTGGAAGAGCAGCTAGATTGAGTAAGGAGATAATGAGAAGAAATAAGATTCTCATAATCCTTGATGACATTTGGGAGAGGATGGATATATCAAGAATAGGAATACCTAGCTATGAGGAACTTCAAAAGTGTAATTCCAAAGTCCTACTCACCACAAGGATATGGAATGTCTGTCATGTCATGAGATGCCAAGAAAAGATCATCCTCAATATCCTATCAAAAGAGGATTCTTGGACATTGTTTGTGAGAAATGCAGGAAGGTCTTTTGAATCCACCAATTTTGAGAATGTAGCGAGGAAGGTAGCTGGAGAATGTAGGGGTCTACCGATTGCATTGATAGCAGTTGCAAGGGCACTCGGCGATAAAGAATTGGTGGAATGGGAAAGAGCAGCTCAACGACTAGAGAAGTCACAAACTGCCAACCCTGACGATAAAGGAGATGCATCTGAATGTATAAGATTAAGTTATGATTACTTGAAAGATGAGGATTACAAGTCATGCTTCTTGCTCTGTTGCTTATTTCCAGAAGACTATGACATCCAAATAGAAAACTTGTTCAACTATGTGATAGGGAATGGATTGTTTAGAGATGCTGAGACCATCGAAGAAGCCAGAGGAACAACAAATTTGGTGGTTGCGCACCTGAAAAATTGTAGCTTGCTTTTGGATAGTGATGAAGATGGATGTGTAAGAATGCATGATGTCATCCGGGATACAGCCATGATAATTGCAAAATCTAAAGACGGGCATGGGTTTTTGGTAAAAGCTGGGTCTGGTTTAAAGCATTGGTCATGTCGATTACATGAAGACTACTCTGCAATTTCACTAATGAAGAACAAAATTCGCAAGCTACCTGAAGAGTTGGTATGTCCAAAACTCCAGATTTTATTACTACAAGAGAATGCTGATTTAACAGAGATTCCAGAAAAATTTCTCCAAAGTGCAAATGAACTAAGGGTCTTAGATCTTGGCCTCACTAGTGTTTTATCACTACCCCAATCATTGAGTCTCCTATCAAACCTCCAAGCTTTGTATTTAGATTTTTGCAAGAAAAGAGTTGACATTTCCATAATTGGAAAACTTACTAAGCTTGAGATTCTTAGTATGAGAGGTTATCCTGTTAAGGAATTGTCAAGAGAATTGTGGAGAGAAGTAGGAAAATTGACTAATCTAAGGATGTTGGATGTCGGTGGTGTTAGTGTGGTGGGTGGAGGTACTGTCACAATTCCCTCTAAAGTGATATCAAAGTTGCATAGATTAGAAGAACTGTACATGCTGTACTGTGGATTTGAGGACTGGGGAAGTCGGGTTGAGGGAGAAGGAGAAGAAACATATATTATCTTTGATGAGTTAACTGGTTTATCAAATTTGAAAATTGTGCAGCTTTGCATATCTGATGCAAAATGCATCCCAAAAGAAGTTGAGGTCGAACCAAATTGGGTTTACTTTGATATACGTATCGATGGCTGGTCAAGCAATCCATACAAGCGAAATGTTCCTAACTCAAGATCCTCGTCTCTCAACACAACCATCAGTAGCTTACCAGACTGGTTTGTCAATGTGGTGACAAGGAAATCAGAGAAGCTATTGTATGAAGAAGACTGCAAAGGTTTAAGTAACATTCTTATGGAATATGACCATGGGAGGTTACATGGACTGAAGCATCTCTCTGTAATTGGTCCCAATAAGAGCTTGAAAGAGTTGATGAACACAACAACACGGATTAAACAAGAACCTGTGTTTGAGAACTTGGAAGAGTTGCATCTGAGTCGTCTGGACTGCATGAAGGAGTTGTGTGTTGGTGACTTACCACCTGGTTCTCTATTCAATGTGAAGTTATTGAGGATCCGCGATTGTCTTAACTTGGGGAATGTACTGTTGCCTTCAAAATTGTTGCAGAGACTGCCAAATCTGGAAATAGTATTTCTTTACAGAATACGTAAATTGGAATTTGTGTTTGAATGCGAAGGGTTTGAGCCAGAACATTCAAAATTGAGAGAGGTGACATTGTTGTGGCTAGATGCAGTAAGAAGCATATGTAATGGTCCGGTTCCCCGTGCTATGTTCCGGACTCTTAGAAGTTTGTTAATTTTCCGTTGTAAGCCTTTGGGAAGTCTCTTCACATGTGATGCAGCGCAATGTCTTTTTCAATTGGAAGAGCTTTGGGTAGAGAATTGCCCTTTGTTGGAAAGAGTAATTGAAACAAGCAAGGAAACAGTTAACAACAACAAGAGGATTGTTCTTTCAAAATTAAATAAGTTAGTTTTGAAGGAACTTCCTGTGTTGTACAGTGGAAGCGCTACTACTGACATTGATATCGAGTGTCCTTCATTGGAACACCTGTATGTGAAGGACTGCCCCCATTTGTCATTTCCAACCTCTGCTTCTGACTATTTCCACAGCAGGAACGAGATCCAACTCAATGATCAACAAGAGCATGACATTCTATATCGCAGAATTTGGAGTCCAACATTGGAAAGCTGA

Protein Analysis

1165

Amino Acids

133.67

Weight (kDa)

5.81

Isoelectric Point (pI)

46.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 77 - 246 5.9e-49 TIR domain
TIR_2 PF13676 80 - 175 1.1e-15 TIR domain
NB-ARC PF00931 260 - 423 1.7e-28 NB-ARC domain
LRR_14 PF23598 634 - 766 6.5e-11 Leucine-rich repeat region
LRR_RPS2 PF23247 921 - 1064 7.5e-18 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1013 - 1133 2.3e-13 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 513
AasI GACNNNNNNGTC 1 cut(s) 2567
Acc16I TGCGCA 1 cut(s) 1653
AccI GTMKAC 2 cut(s) 837, 1336
AccII CGCG 1 cut(s) 2865
AccIII TCCGGA 1 cut(s) 3081
AciI CCGC 2 cut(s) 41, 2863
AclWI GGATC 4 cut(s) 2517, 2854, 2867, 3421
AcoI YGGCCR 1 cut(s) 24
AcuI CTGAAG 2 cut(s) 1890, 2693
AcyI GRCGYC 1 cut(s) 852
AfaI GTAC 6 cut(s) 726, 2230, 2285, 2294, 2889, 3302
AfeI AGCGCT 1 cut(s) 3313
AfiI CCNNNNNNNGG 1 cut(s) 3199
AflIII ACRYGT 1 cut(s) 3137
AhlI ACTAGT 1 cut(s) 1991
AjnI CCWGG 1 cut(s) 2827
AjuI GAANNNNNNNTTGG 4 cut(s) 92, 124, 3382, 3414
Alw26I GTCTC 6 cut(s) 1001, 1604, 2027, 2535, 2908, 3134
AlwI GGATC 4 cut(s) 2517, 2854, 2867, 3421
AlwNI CAGNNNCTG 1 cut(s) 2917
Aor13HI TCCGGA 1 cut(s) 3081
Aor51HI AGCGCT 1 cut(s) 3313
AoxI GGCC 3 cut(s) 24, 967, 1984
ApeKI GCWGC 5 cut(s) 215, 1025, 1403, 2395, 3146
Asp700I GAANNNNTTC 1 cut(s) 1944
AspLEI GCGC 3 cut(s) 1654, 3151, 3314
AspS9I GGNCC 4 cut(s) 257, 607, 2693, 3059
AsuC2I CCSGG 1 cut(s) 1724
AsuHPI GGTGA 6 cut(s) 186, 559, 1156, 2590, 2828, 3028
AsuII TTCGAA 2 cut(s) 555, 993
AvaII GGWCC 4 cut(s) 257, 607, 2693, 3059
BaeGI GKGCMC 1 cut(s) 1369
BalI TGGCCA 1 cut(s) 26
BamHI GGATCC 1 cut(s) 2859
BarI GAAGNNNNNNTAC 2 cut(s) 3301, 3333
BbsI GAAGAC 4 cut(s) 702, 1548, 1827, 2619
BbvI GCAGC 5 cut(s) 227, 1037, 1415, 2407, 3158
BccI CCATC 8 cut(s) 16, 205, 219, 1090, 1622, 1688, 2471, 2552
BcgI CGANNNNNNTGC 6 cut(s) 410, 444, 806, 840, 2765, 2799
BciT130I CCWGG 1 cut(s) 2829
BciVI GTATCC 2 cut(s) 349, 1720
BclI TGATCA 2 cut(s) 886, 3439
BcnI CCSGG 1 cut(s) 1724
BcoDI GTCTC 6 cut(s) 1001, 1604, 2027, 2535, 2908, 3134
BcuI ACTAGT 1 cut(s) 1991
BfaI CTAG 7 cut(s) 95, 806, 1029, 1122, 1415, 1992, 3032
BfmI CTRYAG 1 cut(s) 715
BfoI RGCGCY 1 cut(s) 3315
BfuI GTATCC 2 cut(s) 349, 1720
BglII AGATCT 4 cut(s) 340, 465, 979, 1978
BisI GCNGC 5 cut(s) 216, 1026, 1404, 2396, 3147
BlsI GCNGC 5 cut(s) 217, 1027, 1405, 2397, 3148
Bme1390I CCNGG 2 cut(s) 1724, 2829
Bme18I GGWCC 4 cut(s) 257, 607, 2693, 3059
BmgT120I GGNCC 4 cut(s) 257, 607, 2693, 3059
BmiI GGNNCC 4 cut(s) 258, 2695, 2861, 3066
BmrFI CCNGG 2 cut(s) 1724, 2829
BmrI ACTGGG 1 cut(s) 2320
BmuI ACTGGG 1 cut(s) 2320
BpiI GAAGAC 4 cut(s) 702, 1548, 1827, 2619
BpmI CTGGAG 3 cut(s) 163, 1341, 1877
Bpu14I TTCGAA 2 cut(s) 555, 993
BpuEI CTTGAG 4 cut(s) 265, 973, 2126, 2503
BpuMI CCSGG 1 cut(s) 1724
Bsa29I ATCGAT 2 cut(s) 171, 2475
BsaAI YACGTR 2 cut(s) 2471, 2954
BsaHI GRCGYC 1 cut(s) 852
BsaI GGTCTC 1 cut(s) 1604
BsaJI CCNNGG 3 cut(s) 27, 867, 2653
BsaWI WCCGGW 2 cut(s) 3061, 3081
BsaXI ACNNNNNCTCC 4 cut(s) 349, 379, 2217, 2247
Bsc4I CCNNNNNNNGG 1 cut(s) 3199
Bse1I ACTGG 3 cut(s) 2315, 2374, 2567
Bse3DI GCAATG 1 cut(s) 3158
BseAI TCCGGA 1 cut(s) 3081
BseBI CCWGG 1 cut(s) 2829
BseCI ATCGAT 2 cut(s) 171, 2475
BseDI CCNNGG 3 cut(s) 27, 867, 2653
BseGI GGATG 9 cut(s) 337, 1101, 1215, 1551, 1703, 1719, 2196, 2203, 2421
BseLI CCNNNNNNNGG 1 cut(s) 3199
BseMI GCAATG 1 cut(s) 3158
BseMII CTCAG 2 cut(s) 1599, 2771
BseNI ACTGG 3 cut(s) 2315, 2374, 2567
BseRI GAGGAG 1 cut(s) 1019
BseSI GKGCMC 1 cut(s) 1369
BseXI GCAGC 5 cut(s) 227, 1037, 1415, 2407, 3158
BseYI CCCAGC 1 cut(s) 1781
BsgI GTGCAG 2 cut(s) 287, 2414
Bsh1236I CGCG 1 cut(s) 2865
BshFI GGCC 3 cut(s) 26, 969, 1986
BshVI ATCGAT 2 cut(s) 171, 2475
BsiSI CCGG 3 cut(s) 1723, 3062, 3082
BslFI GGGAC 1 cut(s) 2679
BslI CCNNNNNNNGG 1 cut(s) 3199
BsmAI GTCTC 6 cut(s) 1001, 1604, 2027, 2535, 2908, 3134
BsmBI CGTCTC 1 cut(s) 2535
BsmFI GGGAC 1 cut(s) 2679
BsmI GAATGC 3 cut(s) 1713, 1921, 2981
BsnI GGCC 3 cut(s) 26, 969, 1986
Bso31I GGTCTC 1 cut(s) 1604
Bsp119I TTCGAA 2 cut(s) 555, 993
Bsp1286I GDGCHC 1 cut(s) 1369
Bsp13I TCCGGA 1 cut(s) 3081
Bsp1407I TGTACA 2 cut(s) 2283, 3300
Bsp19I CCATGG 3 cut(s) 27, 867, 2653
BspACI CCGC 2 cut(s) 41, 2863
BspANI GGCC 3 cut(s) 26, 969, 1986
BspCNI CTCAG 2 cut(s) 1600, 2772
BspDI ATCGAT 2 cut(s) 171, 2475
BspEI TCCGGA 1 cut(s) 3081
BspFNI CGCG 1 cut(s) 2865
BspHI TCATGA 1 cut(s) 1194
BspLI GGNNCC 4 cut(s) 258, 2695, 2861, 3066
BspPI GGATC 4 cut(s) 2517, 2854, 2867, 3421
BspQI GCTCTTC 2 cut(s) 1015, 3165
BspT104I TTCGAA 2 cut(s) 555, 993
BspTNI GGTCTC 1 cut(s) 1604
BsrDI GCAATG 1 cut(s) 3158
BsrGI TGTACA 2 cut(s) 2283, 3300
BsrI ACTGG 3 cut(s) 2315, 2374, 2567
BssECI CCNNGG 3 cut(s) 27, 867, 2653
BssNI GRCGYC 1 cut(s) 852
BssT1I CCWWGG 3 cut(s) 27, 867, 2653
Bst2UI CCWGG 1 cut(s) 2829
Bst4CI ACNGT 7 cut(s) 76, 2233, 2283, 2297, 2892, 3232, 3305
Bst6I CTCTTC 7 cut(s) 192, 539, 1015, 1866, 2763, 3137, 3165
BstACI GRCGYC 1 cut(s) 852
BstAPI GCANNNNNTGC 1 cut(s) 2917
BstAUI TGTACA 2 cut(s) 2283, 3300
BstBAI YACGTR 2 cut(s) 2471, 2954
BstBI TTCGAA 2 cut(s) 555, 993
BstC8I GCNNGC 2 cut(s) 1673, 1862
BstDEI CTNAG 8 cut(s) 1608, 1967, 1975, 2100, 2114, 2186, 2780, 3089
BstDSI CCRYGG 3 cut(s) 27, 867, 2653
BstF5I GGATG 9 cut(s) 337, 1101, 1215, 1551, 1703, 1719, 2196, 2203, 2421
BstFNI CGCG 1 cut(s) 2865
BstH2I RGCGCY 1 cut(s) 3315
BstHHI GCGC 3 cut(s) 1654, 3151, 3314
BstMAI GTCTC 6 cut(s) 1001, 1604, 2027, 2535, 2908, 3134
BstMWI GCNNNNNNNGC 5 cut(s) 38, 142, 1352, 2487, 2917
BstNI CCWGG 1 cut(s) 2829
BstNSI RCATGY 2 cut(s) 2290, 3141
BstSCI CCNGG 2 cut(s) 1722, 2827
BstSFI CTRYAG 1 cut(s) 715
BstSLI GKGCMC 1 cut(s) 1369
BstSNI TACGTA 2 cut(s) 2471, 2954
BstUI CGCG 1 cut(s) 2865
BstV1I GCAGC 5 cut(s) 227, 1037, 1415, 2407, 3158
BstV2I GAAGAC 4 cut(s) 702, 1548, 1827, 2619
BstX2I RGATCY 7 cut(s) 340, 465, 979, 1978, 2522, 2859, 3426
BstXI CCANNNNNNTGG 1 cut(s) 790
BstYI RGATCY 7 cut(s) 340, 465, 979, 1978, 2522, 2859, 3426
Bsu15I ATCGAT 2 cut(s) 171, 2475
BsuI GTATCC 2 cut(s) 349, 1720
BsuRI GGCC 3 cut(s) 26, 969, 1986
BsuTUI ATCGAT 2 cut(s) 171, 2475
BtgI CCRYGG 3 cut(s) 27, 867, 2653
BtgZI GCGATG 1 cut(s) 126
BtsCI GGATG 9 cut(s) 337, 1101, 1215, 1551, 1703, 1719, 2196, 2203, 2421
BtsI GCAGTG 1 cut(s) 822
BtsIMutI CAGTG 3 cut(s) 822, 889, 3310
Cac8I GCNNGC 2 cut(s) 1673, 1862
CaiI CAGNNNCTG 1 cut(s) 2917
CciI TCATGA 1 cut(s) 1194
CfoI GCGC 3 cut(s) 1654, 3151, 3314
Cfr13I GGNCC 4 cut(s) 257, 607, 2693, 3059
ClaI ATCGAT 2 cut(s) 171, 2475
CseI GACGC 1 cut(s) 841
CsiI ACCWGGT 1 cut(s) 2827
Csp6I GTAC 6 cut(s) 725, 2229, 2284, 2293, 2888, 3301
CspCI CAANNNNNGTGG 4 cut(s) 1276, 1311, 1626, 1661
CviQI GTAC 6 cut(s) 725, 2229, 2284, 2293, 2888, 3301
DdeI CTNAG 8 cut(s) 1608, 1967, 1975, 2100, 2114, 2186, 2780, 3089
DraI TTTAAA 2 cut(s) 630, 1794
DrdI GACNNNNNNGTC 1 cut(s) 2567
DseDI GACNNNNNNGTC 1 cut(s) 2567
EaeI YGGCCR 1 cut(s) 24
Eam1104I CTCTTC 7 cut(s) 192, 539, 1015, 1866, 2763, 3137, 3165
EarI CTCTTC 7 cut(s) 192, 539, 1015, 1866, 2763, 3137, 3165
Eco105I TACGTA 2 cut(s) 2471, 2954
Eco130I CCWWGG 3 cut(s) 27, 867, 2653
Eco31I GGTCTC 1 cut(s) 1604
Eco32I GATATC 3 cut(s) 539, 2256, 3331
Eco47I GGWCC 4 cut(s) 257, 607, 2693, 3059
Eco47III AGCGCT 1 cut(s) 3313
Eco57I CTGAAG 2 cut(s) 1890, 2693
EcoO109I RGGNCCY 1 cut(s) 257
EcoRI GAATTC 3 cut(s) 5, 495, 995
EcoRII CCWGG 1 cut(s) 2827
EcoRV GATATC 3 cut(s) 539, 2256, 3331
EcoT14I CCWWGG 3 cut(s) 27, 867, 2653
EcoT22I ATGCAT 3 cut(s) 1459, 1713, 2423
ErhI CCWWGG 3 cut(s) 27, 867, 2653
Esp3I CGTCTC 1 cut(s) 2535
FalI AAGNNNNNCTT 2 cut(s) 1499, 1531
FaqI GGGAC 1 cut(s) 2679
FauNDI CATATG 1 cut(s) 3050
FbaI TGATCA 2 cut(s) 886, 3439
FblI GTMKAC 2 cut(s) 837, 1336
Fnu4HI GCNGC 5 cut(s) 216, 1026, 1404, 2396, 3147
FokI GGATG 9 cut(s) 344, 1108, 1202, 1538, 1706, 1710, 2203, 2210, 2408
Fsp4HI GCNGC 5 cut(s) 216, 1026, 1404, 2396, 3147
FspBI CTAG 7 cut(s) 95, 806, 1029, 1122, 1415, 1992, 3032
FspI TGCGCA 1 cut(s) 1653
GlaI GCGC 3 cut(s) 1653, 3150, 3313
GluI GCNGC 5 cut(s) 216, 1026, 1404, 2396, 3147
GsaI CCCAGC 1 cut(s) 1785
GsuI CTGGAG 3 cut(s) 163, 1341, 1877
HaeII RGCGCY 1 cut(s) 3315
HaeIII GGCC 3 cut(s) 26, 969, 1986
HapII CCGG 3 cut(s) 1723, 3062, 3082
HgaI GACGC 1 cut(s) 841
HhaI GCGC 3 cut(s) 1654, 3151, 3314
Hin1I GRCGYC 1 cut(s) 852
Hin6I GCGC 3 cut(s) 1652, 3149, 3312
HinP1I GCGC 3 cut(s) 1652, 3149, 3312
HincII GTYRAC 4 cut(s) 166, 2074, 2367, 3235
HindII GTYRAC 4 cut(s) 166, 2074, 2367, 3235
HindIII AAGCTT 3 cut(s) 561, 2040, 2102
HpaI GTTAAC 2 cut(s) 2367, 3235
HpaII CCGG 3 cut(s) 1723, 3062, 3082
HphI GGTGA 6 cut(s) 186, 559, 1156, 2590, 2828, 3028
Hpy166II GTNNAC 7 cut(s) 166, 838, 1337, 2074, 2367, 2458, 3235
Hpy188I TCNGA 8 cut(s) 421, 543, 601, 1462, 2410, 2593, 2781, 3403
Hpy8I GTNNAC 7 cut(s) 166, 838, 1337, 2074, 2367, 2458, 3235
Hpy99I CGWCG 2 cut(s) 225, 857
HpyCH4III ACNGT 7 cut(s) 76, 2233, 2283, 2297, 2892, 3232, 3305
HpyCH4IV ACGT 2 cut(s) 2470, 2953
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 142, 1352, 2487, 2917
HpyF3I CTNAG 8 cut(s) 1608, 1967, 1975, 2100, 2114, 2186, 2780, 3089
HpySE526I ACGT 2 cut(s) 2470, 2953
Hsp92I GRCGYC 1 cut(s) 852
HspAI GCGC 3 cut(s) 1652, 3149, 3312
Kpn2I TCCGGA 1 cut(s) 3081
Ksp22I TGATCA 2 cut(s) 886, 3439
KspAI GTTAAC 2 cut(s) 2367, 3235
LguI GCTCTTC 2 cut(s) 1015, 3165
Lsp1109I GCAGC 5 cut(s) 227, 1037, 1415, 2407, 3158
MabI ACCWGGT 1 cut(s) 2827
MaeI CTAG 7 cut(s) 95, 806, 1029, 1122, 1415, 1992, 3032
MaeII ACGT 2 cut(s) 2470, 2953
MfeI CAATTG 4 cut(s) 384, 405, 971, 3164
MflI RGATCY 7 cut(s) 340, 465, 979, 1978, 2522, 2859, 3426
MhlI GDGCHC 1 cut(s) 1369
MlsI TGGCCA 1 cut(s) 26
MluNI TGGCCA 1 cut(s) 26
MlyI GAGTC 6 cut(s) 85, 843, 2029, 2791, 3079, 3487
MmeI TCCRAC 5 cut(s) 634, 2175, 3180, 3413, 3454
Mox20I TGGCCA 1 cut(s) 26
Mph1103I ATGCAT 3 cut(s) 1459, 1713, 2423
MroI TCCGGA 1 cut(s) 3081
MroXI GAANNNNTTC 1 cut(s) 1944
MscI TGGCCA 1 cut(s) 26
MslI CAYNNNNRTG 6 cut(s) 788, 882, 1462, 2639, 3025, 3357
Msp20I TGGCCA 1 cut(s) 26
MspA1I CMGCKG 1 cut(s) 203
MspI CCGG 3 cut(s) 1723, 3062, 3082
MspR9I CCNGG 2 cut(s) 1724, 2829
MunI CAATTG 4 cut(s) 384, 405, 971, 3164
Mva1269I GAATGC 3 cut(s) 1713, 1921, 2981
MvaI CCWGG 1 cut(s) 2829
MvnI CGCG 1 cut(s) 2865
MwoI GCNNNNNNNGC 5 cut(s) 38, 142, 1352, 2487, 2917
NciI CCSGG 1 cut(s) 1724
NcoI CCATGG 3 cut(s) 27, 867, 2653
NdeI CATATG 1 cut(s) 3050
NlaIV GGNNCC 4 cut(s) 258, 2695, 2861, 3066
NmeAIII GCCGAG 1 cut(s) 1350
NmuCI GTSAC 7 cut(s) 49, 820, 1422, 2233, 2578, 2816, 3016
NsbI TGCGCA 1 cut(s) 1653
NsiI ATGCAT 3 cut(s) 1459, 1713, 2423
NspI RCATGY 2 cut(s) 2290, 3141
NspV TTCGAA 2 cut(s) 555, 993
PagI TCATGA 1 cut(s) 1194
PciI ACATGT 1 cut(s) 3137
PciSI GCTCTTC 2 cut(s) 1015, 3165
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PctI GAATGC 3 cut(s) 1713, 1921, 2981
PdmI GAANNNNTTC 1 cut(s) 1944
PfeI GAWTC 9 cut(s) 206, 392, 659, 906, 1063, 1241, 1283, 1933, 2110
PflFI GACNNNGTC 1 cut(s) 818
PfoI TCCNGGA 1 cut(s) 1722
PkrI GCNGC 5 cut(s) 217, 1027, 1405, 2397, 3148
PleI GAGTC 6 cut(s) 85, 842, 2028, 2790, 3079, 3486
PpsI GAGTC 6 cut(s) 85, 842, 2028, 2790, 3079, 3486
Ppu21I YACGTR 2 cut(s) 2471, 2954
PpuMI RGGWCCY 1 cut(s) 257
PscI ACATGT 1 cut(s) 3137
PsiI TTATAA 1 cut(s) 513
Psp5II RGGWCCY 1 cut(s) 257
Psp6I CCWGG 1 cut(s) 2827
PspFI CCCAGC 1 cut(s) 1781
PspGI CCWGG 1 cut(s) 2827
PspN4I GGNNCC 4 cut(s) 258, 2695, 2861, 3066
PspPI GGNCC 4 cut(s) 257, 607, 2693, 3059
PspPPI RGGWCCY 1 cut(s) 257
PstNI CAGNNNCTG 1 cut(s) 2917
PsuI RGATCY 7 cut(s) 340, 465, 979, 1978, 2522, 2859, 3426
PsyI GACNNNGTC 1 cut(s) 818
PvuII CAGCTG 1 cut(s) 203
RsaI GTAC 6 cut(s) 726, 2230, 2285, 2294, 2889, 3302
RsaNI GTAC 6 cut(s) 725, 2229, 2284, 2293, 2888, 3301
RseI CAYNNNNRTG 6 cut(s) 788, 882, 1462, 2639, 3025, 3357
SapI GCTCTTC 2 cut(s) 1015, 3165
SatI GCNGC 5 cut(s) 216, 1026, 1404, 2396, 3147
Sau96I GGNCC 4 cut(s) 257, 607, 2693, 3059
SchI GAGTC 6 cut(s) 85, 843, 2029, 2791, 3079, 3487
ScrFI CCNGG 2 cut(s) 1724, 2829
SduI GDGCHC 1 cut(s) 1369
SexAI ACCWGGT 1 cut(s) 2827
SfcI CTRYAG 1 cut(s) 715
SfuI TTCGAA 2 cut(s) 555, 993
SinI GGWCC 4 cut(s) 257, 607, 2693, 3059
SmiMI CAYNNNNRTG 6 cut(s) 788, 882, 1462, 2639, 3025, 3357
SmlI CTYRAG 4 cut(s) 244, 952, 2105, 2518
SmoI CTYRAG 4 cut(s) 244, 952, 2105, 2518
SnaBI TACGTA 2 cut(s) 2471, 2954
SpeI ACTAGT 1 cut(s) 1991
SsiI CCGC 2 cut(s) 41, 2863
SspMI CTAG 7 cut(s) 95, 806, 1029, 1122, 1415, 1992, 3032
StyD4I CCNGG 2 cut(s) 1722, 2827
StyI CCWWGG 3 cut(s) 27, 867, 2653
TaaI ACNGT 7 cut(s) 76, 2233, 2283, 2297, 2892, 3232, 3305
TaiI ACGT 2 cut(s) 2473, 2956
TatI WGTACW 5 cut(s) 724, 2283, 2292, 2887, 3300
TfiI GAWTC 9 cut(s) 206, 392, 659, 906, 1063, 1241, 1283, 1933, 2110
TscAI CASTG 3 cut(s) 829, 889, 3310
TseFI GTSAC 7 cut(s) 49, 820, 1422, 2233, 2578, 2816, 3016
TseI GCWGC 5 cut(s) 215, 1025, 1403, 2395, 3146
Tsp45I GTSAC 7 cut(s) 49, 820, 1422, 2233, 2578, 2816, 3016
TspGWI ACGGA 3 cut(s) 855, 2749, 3098
TspRI CASTG 3 cut(s) 829, 889, 3310
Tth111I GACNNNGTC 1 cut(s) 818
VpaK11BI GGWCC 4 cut(s) 257, 607, 2693, 3059
XceI RCATGY 2 cut(s) 2290, 3141
XmiI GTMKAC 2 cut(s) 837, 1336
XmnI GAANNNNTTC 1 cut(s) 1944
XspI CTAG 7 cut(s) 95, 806, 1029, 1122, 1415, 1992, 3032
Zsp2I ATGCAT 3 cut(s) 1459, 1713, 2423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.