Rroxscaffold_1G00019420

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000085
Physical Location & Seq
Forward (+)
23730197 .. 23737419
7223 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00019420.1

Sequence Viewer

Length: 4401 bp
ATGAAATGGAGAGACGCCTTGGACAAAATCAGCAGCATCTTTGGATGGGTATGTGGGAGTTCTGAGCCAGAAGGACACGTTGTCCAACAAATCGCGGAATTTGTCTGCAGCAAACCCATTGAAATTGAGTCGACGGAAGATATTGAAACAAAACAAGCAATGAATGGAACTTCTCCTGGTTGGAAGTATGATGTGTTTATAAATTTCAGGGGTGAGGACACTTGTAAGAGTTTTCTGTCCTATTTACTAGCTGAATTGCAAGAGAAACCAGCACACCTGAACACAGTAAAGAATGATGAACCACTTCCAAAAGGGCGGAATATATCTTCGTATCTCCTAAACGCCATAGAAGAGGTGAGGTTTGCCATTGTTGTTCTTTCGCAAGACTTTGCTTCTTCATCATGGTGCCTAGAGGAACTAGCAAAGATTTTTGAATGCATGGGAAACCAAAATAGAATTCTGCCAATCTTTTATCATGTGGATCCCTCTGACGTGCGACATCAAAGGGGGAGTTTTGGAGATGCCTTCGCTGTACATGAAGAAAGATTTCGGGATAATCATGAGAAGGTCAGGCGGTGGAGACATGTTTTATCAATGGTGGCCAATCTCTCTGGATGGAATTCAGAGGATTATGAGTATGAAGGAGAACTTATCAAAGACGTTGTGCAAGCTATGTGCAGCAGACTAGTTCAGTCTACAGAGTCGACAGAAGATTTTGAAGCAACAGGAGATAACATGGACCGACCTTCTCCCCAGCCTGACCCAGCTCCTGGGTCGAAGAATGAAGTGTTTTCAGGTTTCAGGATGGAAGACACTCCCAGGGGTTTTGTGCCCCATTCAGAACATGAATTGCCAAACACAGGACTAAGTAAAACATTCAAGGATGAAGACCAGCTTGAAAAAGGGGTAGCCCTCCCCTCTGCATCTGTTTCCTCAACTGAACCCGCTCCTCAACCAGCTCTTGGGCAAAAGAATGATGAGTTTTTGGGTTTCAGGGGAGAAGACACTCATAGGGATTTTGTCACCCATTCAAGTCATGAAATACAAAACACCAGGGTGACTAAGACATTCACCGATGAAGAACATCTTGAAAAAGGGATAGCTGCGTCTCCGCCTTCATCAGCTGAACGAGATCCTCGGCCGAAGTATGATGTGTTTTTGAGCTTTAGGGGTGAAGACACTCGTAAGGGTTTTATAGCCTATTTATATCGTGAATTGCAAAACACCAGAGTAATTAGAACTTTCAAGGATGACATACAACTACAAAAAGGGACGGTTATTTCTTCAAATCTCCTAACGGCAATCGAAGAATCAAGGTTTGCGATTATTGTGTTGTCGGAGAACTATGCTTCTTCTTCATGGTGTTTGGATGAACTTACAAAGATTTTTCAGTGCATGGAGGGTAAGGACACAATTCTCCCAATTTTTTATAAGTTGGATCCCGCTGTTGTACGAACTCAAAATGAAGAGACAAGTTTTGGAGAAGCTTTCATTAAGCATGAAGCTACACAAGACATTGAGAAGATGAACCAGTGGAGAGCTGCTTTAAAAGGAGTGGCTGGAATCTCTGGGTGGCATTCAACCAACTATCCGACGGATATAGAACTAATTGAAGACATTGTGAAATATTTGTGGGAGAAAGTACTTGAAGCACCCACCGGAAATTTTGAAACATTTGAAGCGACAAGGCAAGCCATGGATGAGGTTATGAAGGTATTAAAAGATGATCAAGTCACTGTCCTTGGGGTGCATGGAGTGGCTGGCGTTGGAAAGACAATGATGGTCAAAAATGTTAGTGCAGAAGCCCAGAAAGGTGGGCTTTTTGATCATGTGATTATGGCTGTCGTATCCAAAACCCCTGACTTGCAAGAAATTCAAGGCACATTGGCAGATTCGCTGGGCTTGGAGGAGGGGATCATGAGAGGAAGCAGCATGCTTATAATCTTGGACGACATTTGGGAGAGTTTTGAACTCTCGAGCATTGGAATTCCCAATCACAATGAGCTTCAAAGGTGCAATTCTAAAGTTATACTTACCACAAGGAAAAGCAATATATGCCATTCTATGGGGTGCCATGCCCAGATCCCTCTCAATGTTCTATCAGAGGGAGATTCGTGGAAGTTATTCATGAAGACGTCGGGGAAGTCTTTTCATGAATCTACCGTTTCCTATGATGAAGCGAGAAAGATAGCTGGAGAATGCGGTGGTCTCCCAAAAGCAATAAAAGCAGCTGCAAGGTCACTTGGAGATAAATACTTGGACGAATGGAATGAAGCAGTTGAAACAGCATTTGGAGATGACTGCTTGGACGAATGGAATGAAACAGCTCATCCTGTCAACTTCGAAGCTGAGGAAGACGTATTTCATTCATTGGCAACTGATGATGCCAAGTCATGCTTCTTGCTATGCTGCCTATTCCCAGAAAATTATGATATCCCAATTGAAGTGTTGCTCAGGTATGGGATTGGAATAGGTTTGTTTCAATATGATACAATGCAAGAAGCCAGAGATAGAGCACATTCTGTGGTCAAGTACCTCAAAGCTTCTAGATTACTTTTGGACGGTACAAAGAAGGGATGCACAAGGATGACTGATGACATTCGAAATCTGGCCATGTCCATTATGCGGTCTGAAGAAGGTGATGGGTTCTTAGTAAAAGCTGGTTGTGAACTAGAGGAGTGGCCAGAGGCAGGATTTTGGCAAGGAATTACTTTCCGGCTCCGCAAAATCTGGGATTGGCCAATAGATGCTCATGAAGGCTACTCAATAATCTCACTCATGAAAAACAAGCTTCATAAGCTTCCCGAATATTTGGTATGTTCAGAGCTCCAAATTTTATTGTTACAAAGTAATGCTGGTATACGTGACATCCCAGAAACCTTTCTTCAAAGTCCTAATGCGCTAAGGGTCTTTGATCTTAGCTTCACTAGCATTTCATCACTACCTTCATCATTCAGTTTCCTAACCAACCTTCAAGTTTTGAATTTGGATTTTTGCCATAATTTAAGTAACATATCCGTTCTAGGAAAACTTAAGAAGCTTGAGATTCTTAGTATGAGAGAACTTCCTCTTAAGAAATTTCCAAAACAAATAGGAGATTTGACCAGTCTAAGGATGCTGGACGTCACTGGTAGAGATCTTGAAACAATCCCATCTAAAGTGATATCGAGGTTGTATAGATTGGAAGAACTGCACATGAAATTTCAGTTTGGGAAGTGGGGAAGTAAAATTGAGGATGCAGTTGAAGAAACCAATGCTGACTTTGATGAGTTGACCGGCTTGTTGGATTTAAACATTTTGGGAGTTTACATATCTGATGCTGCATGCTTGCCTAAAAGTGTTGAGTACAAGCTGAATTGGGTAGAATTTGATATATGCATCAGCAGAGATGCCCCTTGCGTGTCACTCCCTCAACATGATGGTTTTTCAAGAACCTTGACTCTTGACACAACAATCAATAGCTTACCAGATTGGTTTATCAGCGTTGTGACAGAGAAAGTGGAGAAGCTAGAGTACACAGATTGTGAAGACTTGAATGACATCGTTGGGCAATATGACCATGGCAGGTTACTTGCACTGAAATATCTCTCTGTGAACGGGCACCATAAGAACTTGAAAGAGATAATCAACACAATGACACGGGTTTCAAATAAACCAGTATTTGAGAATTTAGAACAGTTGCATCTGTACCGCTTATATTCCCTCAAGGAGTTATGTGTTGGTGAGCTACCACCTGGGTCTCTACACAAGCTAAAGTTGTTGAAAGTGCAAACTTGTTGTAGCTTGGTTAATACACTTTTACCATCGACATTTTTGCAGAGACTACAAAATCTGGAAGAAATCATTTGTGTAAATCTAGAAGGAATGAAATATGTATTTGGATCTGATGAAGGGCTTGATCCAGAAAATATTTTCTTGAGGAAATTGAGGGAAATGAGATTGTCTACTCTAGACAAACTAGTAAGCATATGTAATGGTCCTGCTCCGCATGCAATCTTCCATAATCTTAAAGTTTTGGCAATTTGCAAATGCAAGGAATTGAAAAATCTCTTCACAATAGGTGTAGCTGAATGTCTTTCTCAGTTGGAAGACCTTTCCGTACAGCAGTGTCATAGCTTGGACAGAGTCATTGAAGTAAGCAATAGCTTGAACAGCAAGATTAAACTTCCGGCGTTGAAGAAATTATCTTTGATAGATCTTCCGGTGCTCACAAGGTTCTGCACTGAAAGTGTTACTATTGAAACTGAGTGCCCTTCATTGGAATACTTGTACGTGGAGAAGTGCCTCCAGTTTTCATATTTTGCTTACGACTTCGACAACAAGAACCAAGTCCACTTCAATGATAAACAACATTTGAGTTCTTTAGTGAAAAGGTGGGAGGAAGTGCATAATCGGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1466

Amino Acids

166.06

Weight (kDa)

5.16

Isoelectric Point (pI)

43.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 62 - 240 2.8e-46 TIR domain
TIR_2 PF13676 65 - 162 5.3e-09 TIR domain
TIR PF01582 382 - 553 4.5e-46 TIR domain
TIR_2 PF13676 385 - 481 6.4e-13 TIR domain
NB-ARC PF00931 564 - 713 9.2e-24 NB-ARC domain
LRR_8 PF13855 942 - 1000 1.2e-07 Leucine rich repeat
LRR_14 PF23598 971 - 1067 2.7e-11 Leucine-rich repeat region
LRR_RPS2 PF23247 1224 - 1370 6.4e-24 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1356 - 1431 7.7e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 200, 1431, 1940
AatII GACGTC 2 cut(s) 2138, 3126
Acc36I ACCTGC 1 cut(s) 3555
AccB1I GGYRCC 3 cut(s) 405, 2070, 3600
AccB7I CCANNNNNTGG 3 cut(s) 770, 962, 2065
AccBSI CCGCTC 1 cut(s) 947
AccI GTMKAC 5 cut(s) 131, 695, 704, 2860, 3944
AccII CGCG 1 cut(s) 95
AclWI GGATC 9 cut(s) 476, 489, 1127, 1433, 1446, 1922, 2077, 3889, 3893
AcoI YGGCCR 5 cut(s) 600, 1139, 2610, 2681, 2738
AcuI CTGAAG 1 cut(s) 2652
AcyI GRCGYC 3 cut(s) 15, 2135, 3123
AfiI CCNNNNNNNGG 8 cut(s) 770, 860, 962, 2065, 2625, 2690, 3111, 4258
AflII CTTAAG 2 cut(s) 3032, 3071
AflIII ACRYGT 2 cut(s) 76, 583
AhdI GACNNNNNGTC 1 cut(s) 80
AhlI ACTAGT 2 cut(s) 685, 3958
AjiI CACGTC 1 cut(s) 493
AjnI CCWGG 5 cut(s) 175, 769, 818, 1052, 3733
AjuI GAANNNNNNNTTGG 6 cut(s) 441, 473, 2274, 2306, 2381, 2413
AleI CACNNNNGTG 1 cut(s) 1055
Alw21I GWGCWC 3 cut(s) 2518, 2829, 4209
Alw26I GTCTC 7 cut(s) 6, 574, 1113, 1463, 2213, 3744, 3814
AlwI GGATC 9 cut(s) 476, 489, 1127, 1433, 1446, 1922, 2077, 3889, 3893
AlwNI CAGNNNCTG 1 cut(s) 770
Ama87I CYCGRG 1 cut(s) 1975
AoxI GGCC 5 cut(s) 600, 1139, 2610, 2681, 2738
Asp700I GAANNNNTTC 4 cut(s) 303, 546, 2123, 2880
AspLEI GCGC 1 cut(s) 2902
AspS9I GGNCC 2 cut(s) 739, 3977
AsuHPI GGTGA 8 cut(s) 224, 367, 1015, 1063, 1069, 1184, 2651, 3734
AsuII TTCGAA 2 cut(s) 2343, 2602
AvaI CYCGRG 1 cut(s) 1975
AvaII GGWCC 2 cut(s) 739, 3977
BaeGI GKGCMC 3 cut(s) 834, 3603, 4253
BalI TGGCCA 4 cut(s) 602, 2612, 2683, 2740
BamHI GGATCC 2 cut(s) 481, 1438
BanI GGYRCC 3 cut(s) 405, 2070, 3600
BanII GRGCYC 1 cut(s) 2829
BarI GAAGNNNNNNTAC 2 cut(s) 3497, 3529
BbsI GAAGAC 9 cut(s) 816, 894, 1008, 1182, 1620, 2138, 2361, 3534, 4095
Bbv12I GWGCWC 3 cut(s) 2518, 2829, 4209
BbvCI CCTCAGC 1 cut(s) 2349
BccI CCATC 8 cut(s) 39, 609, 799, 1774, 2636, 3160, 3413, 3811
BceAI ACGGC 1 cut(s) 1314
BcgI CGANNNNNNTGC 2 cut(s) 3796, 3830
BciT130I CCWGG 5 cut(s) 177, 771, 820, 1054, 3735
BciVI GTATCC 1 cut(s) 1858
BclI TGATCA 2 cut(s) 1726, 1825
BcoDI GTCTC 7 cut(s) 6, 574, 1113, 1463, 2213, 3744, 3814
BcuI ACTAGT 2 cut(s) 685, 3958
BfmI CTRYAG 2 cut(s) 106, 696
BfrI CTTAAG 2 cut(s) 3032, 3071
BfuAI ACCTGC 1 cut(s) 3555
BfuI GTATCC 1 cut(s) 1858
BglII AGATCT 2 cut(s) 3136, 4195
BmcAI AGTACT 1 cut(s) 1644
Bme1390I CCNGG 5 cut(s) 177, 771, 820, 1054, 3735
Bme18I GGWCC 2 cut(s) 739, 3977
BmeRI GACNNNNNGTC 1 cut(s) 80
BmeT110I CYCGRG 1 cut(s) 1975
BmgBI CACGTC 1 cut(s) 493
BmgT120I GGNCC 2 cut(s) 739, 3977
BmiI GGNNCC 6 cut(s) 407, 483, 1440, 2072, 2720, 3602
BmrFI CCNGG 5 cut(s) 177, 771, 820, 1054, 3735
BpiI GAAGAC 9 cut(s) 816, 894, 1008, 1182, 1620, 2138, 2361, 3534, 4095
BpmI CTGGAG 2 cut(s) 2214, 4271
Bpu10I CCTNAGC 3 cut(s) 2349, 2453, 2903
Bpu14I TTCGAA 2 cut(s) 2343, 2602
BpuEI CTTGAG 3 cut(s) 3062, 3689, 3937
BsaAI YACGTR 2 cut(s) 2864, 4273
BsaHI GRCGYC 3 cut(s) 15, 2135, 3123
BsaI GGTCTC 2 cut(s) 2213, 3744
BsaWI WCCGGW 2 cut(s) 1658, 4201
Bsc4I CCNNNNNNNGG 8 cut(s) 770, 860, 962, 2065, 2625, 2690, 3111, 4258
Bse118I RCCGGY 1 cut(s) 3275
Bse1I ACTGG 5 cut(s) 1531, 3105, 3133, 3656, 4288
Bse3DI GCAATG 1 cut(s) 165
BseBI CCWGG 5 cut(s) 177, 771, 820, 1054, 3735
BseLI CCNNNNNNNGG 8 cut(s) 770, 860, 962, 2065, 2625, 2690, 3111, 4258
BseMI GCAATG 1 cut(s) 165
BseMII CTCAG 5 cut(s) 54, 2340, 2467, 4094, 4236
BseNI ACTGG 5 cut(s) 1531, 3105, 3133, 3656, 4288
BseRI GAGGAG 3 cut(s) 939, 1922, 2690
BseSI GKGCMC 3 cut(s) 834, 3603, 4253
BseX3I CGGCCG 1 cut(s) 1139
BseYI CCCAGC 3 cut(s) 753, 763, 1897
BsgI GTGCAG 4 cut(s) 697, 1818, 3176, 4204
Bsh1236I CGCG 1 cut(s) 95
Bsh1285I CGRYCG 1 cut(s) 1142
BshFI GGCC 5 cut(s) 602, 1141, 2612, 2683, 2740
BshNI GGYRCC 3 cut(s) 405, 2070, 3600
BsiEI CGRYCG 1 cut(s) 1142
BsiHKAI GWGCWC 3 cut(s) 2518, 2829, 4209
BsiHKCI CYCGRG 1 cut(s) 1975
BsiSI CCGG 5 cut(s) 1659, 2716, 3276, 4169, 4202
BslFI GGGAC 1 cut(s) 1285
BslI CCNNNNNNNGG 8 cut(s) 770, 860, 962, 2065, 2625, 2690, 3111, 4258
BsmAI GTCTC 7 cut(s) 6, 574, 1113, 1463, 2213, 3744, 3814
BsmBI CGTCTC 2 cut(s) 6, 1113
BsmFI GGGAC 1 cut(s) 1285
BsmI GAATGC 3 cut(s) 440, 1576, 2204
BsnI GGCC 5 cut(s) 602, 1141, 2612, 2683, 2740
Bso31I GGTCTC 2 cut(s) 2213, 3744
BsoBI CYCGRG 1 cut(s) 1975
Bsp119I TTCGAA 2 cut(s) 2343, 2602
Bsp1286I GDGCHC 6 cut(s) 834, 2518, 2829, 3603, 4209, 4253
Bsp1407I TGTACA 1 cut(s) 532
Bsp19I CCATGG 2 cut(s) 1695, 3559
BspANI GGCC 5 cut(s) 602, 1141, 2612, 2683, 2740
BspCNI CTCAG 5 cut(s) 55, 2341, 2466, 4093, 4237
BspFNI CGCG 1 cut(s) 95
BspHI TCATGA 7 cut(s) 559, 1036, 1917, 2127, 2152, 2752, 2778
BspLI GGNNCC 6 cut(s) 407, 483, 1440, 2072, 2720, 3602
BspMAI CTGCAG 1 cut(s) 110
BspMI ACCTGC 1 cut(s) 3555
BspPI GGATC 9 cut(s) 476, 489, 1127, 1433, 1446, 1922, 2077, 3889, 3893
BspT104I TTCGAA 2 cut(s) 2343, 2602
BspT107I GGYRCC 3 cut(s) 405, 2070, 3600
BspTI CTTAAG 2 cut(s) 3032, 3071
BspTNI GGTCTC 2 cut(s) 2213, 3744
BsrBI CCGCTC 1 cut(s) 947
BsrDI GCAATG 1 cut(s) 165
BsrFI RCCGGY 1 cut(s) 3275
BsrGI TGTACA 1 cut(s) 532
BsrI ACTGG 5 cut(s) 1531, 3105, 3133, 3656, 4288
BssAI RCCGGY 1 cut(s) 3275
BssNAI GTATAC 1 cut(s) 2861
BssNI GRCGYC 3 cut(s) 15, 2135, 3123
BssT1I CCWWGG 4 cut(s) 18, 1695, 1741, 3559
Bst1107I GTATAC 1 cut(s) 2861
Bst2UI CCWGG 5 cut(s) 177, 771, 820, 1054, 3735
Bst4CI ACNGT 6 cut(s) 286, 1276, 1738, 2164, 2564, 3678
Bst6I CTCTTC 3 cut(s) 345, 1461, 4055
BstACI GRCGYC 3 cut(s) 15, 2135, 3123
BstAFI CTTAAG 2 cut(s) 3032, 3071
BstAPI GCANNNNNTGC 1 cut(s) 2055
BstAUI TGTACA 1 cut(s) 532
BstBAI YACGTR 2 cut(s) 2864, 4273
BstBI TTCGAA 2 cut(s) 2343, 2602
BstC8I GCNNGC 7 cut(s) 669, 1692, 1762, 1934, 3325, 3329, 3990
BstDSI CCRYGG 2 cut(s) 1695, 3559
BstFNI CGCG 1 cut(s) 95
BstHHI GCGC 1 cut(s) 2902
BstMAI GTCTC 7 cut(s) 6, 574, 1113, 1463, 2213, 3744, 3814
BstMCI CGRYCG 1 cut(s) 1142
BstMWI GCNNNNNNNGC 7 cut(s) 2055, 2225, 2797, 2928, 3989, 4152, 4393
BstNI CCWGG 5 cut(s) 177, 771, 820, 1054, 3735
BstNSI RCATGY 4 cut(s) 587, 1936, 3327, 3992
BstSCI CCNGG 5 cut(s) 175, 769, 818, 1052, 3733
BstSFI CTRYAG 2 cut(s) 106, 696
BstSLI GKGCMC 3 cut(s) 834, 3603, 4253
BstUI CGCG 1 cut(s) 95
BstV2I GAAGAC 9 cut(s) 816, 894, 1008, 1182, 1620, 2138, 2361, 3534, 4095
BstX2I RGATCY 7 cut(s) 481, 1132, 1438, 2082, 3136, 3881, 4195
BstXI CCANNNNNNTGG 1 cut(s) 1814
BstYI RGATCY 7 cut(s) 481, 1132, 1438, 2082, 3136, 3881, 4195
BstZ17I GTATAC 1 cut(s) 2861
BstZI CGGCCG 1 cut(s) 1139
BsuI GTATCC 1 cut(s) 1858
BsuRI GGCC 5 cut(s) 602, 1141, 2612, 2683, 2740
BtgI CCRYGG 2 cut(s) 1695, 3559
BtrI CACGTC 1 cut(s) 493
BtsI GCAGTG 1 cut(s) 4112
BtsIMutI CAGTG 7 cut(s) 1397, 1538, 1734, 3126, 3575, 4112, 4221
BveI ACCTGC 1 cut(s) 3555
Cac8I GCNNGC 7 cut(s) 669, 1692, 1762, 1934, 3325, 3329, 3990
CaiI CAGNNNCTG 1 cut(s) 770
CciI TCATGA 7 cut(s) 559, 1036, 1917, 2127, 2152, 2752, 2778
CfoI GCGC 1 cut(s) 2902
Cfr10I RCCGGY 1 cut(s) 3275
Cfr13I GGNCC 2 cut(s) 739, 3977
CseI GACGC 2 cut(s) 23, 1095
DraI TTTAAA 2 cut(s) 1548, 3291
DriI GACNNNNNGTC 1 cut(s) 80
EaeI YGGCCR 5 cut(s) 600, 1139, 2610, 2681, 2738
EagI CGGCCG 1 cut(s) 1139
Eam1104I CTCTTC 3 cut(s) 345, 1461, 4055
Eam1105I GACNNNNNGTC 1 cut(s) 80
EarI CTCTTC 3 cut(s) 345, 1461, 4055
EciI GGCGGA 2 cut(s) 331, 1101
Ecl136II GAGCTC 1 cut(s) 2827
EclXI CGGCCG 1 cut(s) 1139
Eco130I CCWWGG 4 cut(s) 18, 1695, 1741, 3559
Eco24I GRGCYC 1 cut(s) 2829
Eco31I GGTCTC 2 cut(s) 2213, 3744
Eco32I GATATC 2 cut(s) 2434, 3165
Eco47I GGWCC 2 cut(s) 739, 3977
Eco52I CGGCCG 1 cut(s) 1139
Eco53kI GAGCTC 1 cut(s) 2827
Eco57I CTGAAG 1 cut(s) 2652
Eco88I CYCGRG 1 cut(s) 1975
EcoICRI GAGCTC 1 cut(s) 2827
EcoRI GAATTC 3 cut(s) 456, 619, 1986
EcoRII CCWGG 5 cut(s) 175, 769, 818, 1052, 3733
EcoRV GATATC 2 cut(s) 2434, 3165
EcoT14I CCWWGG 4 cut(s) 18, 1695, 1741, 3559
EcoT22I ATGCAT 2 cut(s) 440, 3380
EcoT38I GRGCYC 1 cut(s) 2829
ErhI CCWWGG 4 cut(s) 18, 1695, 1741, 3559
Esp3I CGTCTC 2 cut(s) 6, 1113
FaqI GGGAC 1 cut(s) 1285
FauI CCCGC 2 cut(s) 952, 1450
FauNDI CATATG 1 cut(s) 3968
FbaI TGATCA 2 cut(s) 1726, 1825
FblI GTMKAC 5 cut(s) 131, 695, 704, 2860, 3944
FriOI GRGCYC 1 cut(s) 2829
GlaI GCGC 1 cut(s) 2901
GsaI CCCAGC 3 cut(s) 757, 767, 1901
GsuI CTGGAG 2 cut(s) 2214, 4271
HaeIII GGCC 5 cut(s) 602, 1141, 2612, 2683, 2740
HapII CCGG 5 cut(s) 1659, 2716, 3276, 4169, 4202
HgaI GACGC 2 cut(s) 23, 1095
HhaI GCGC 1 cut(s) 2902
Hin1I GRCGYC 3 cut(s) 15, 2135, 3123
Hin6I GCGC 1 cut(s) 2900
HinP1I GCGC 1 cut(s) 2900
HincII GTYRAC 4 cut(s) 132, 705, 2338, 3273
HindII GTYRAC 4 cut(s) 132, 705, 2338, 3273
HindIII AAGCTT 5 cut(s) 1485, 2541, 2789, 2798, 3038
HpaII CCGG 5 cut(s) 1659, 2716, 3276, 4169, 4202
HphI GGTGA 8 cut(s) 224, 367, 1015, 1063, 1069, 1184, 2651, 3734
Hpy99I CGWCG 3 cut(s) 136, 1597, 2140
HpyCH4III ACNGT 6 cut(s) 286, 1276, 1738, 2164, 2564, 3678
HpyCH4IV ACGT 8 cut(s) 78, 492, 660, 2135, 2358, 2863, 3123, 4272
HpyF10VI GCNNNNNNNGC 7 cut(s) 2055, 2225, 2797, 2928, 3989, 4152, 4393
HpySE526I ACGT 8 cut(s) 78, 492, 660, 2135, 2358, 2863, 3123, 4272
Hsp92I GRCGYC 3 cut(s) 15, 2135, 3123
HspAI GCGC 1 cut(s) 2900
Ksp22I TGATCA 2 cut(s) 1726, 1825
LmnI GCTCC 5 cut(s) 772, 952, 2724, 2832, 3988
MaeII ACGT 8 cut(s) 78, 492, 660, 2135, 2358, 2863, 3123, 4272
MbiI CCGCTC 1 cut(s) 947
MfeI CAATTG 1 cut(s) 2439
MflI RGATCY 7 cut(s) 481, 1132, 1438, 2082, 3136, 3881, 4195
MhlI GDGCHC 6 cut(s) 834, 2518, 2829, 3603, 4209, 4253
MlsI TGGCCA 4 cut(s) 602, 2612, 2683, 2740
MluNI TGGCCA 4 cut(s) 602, 2612, 2683, 2740
MlyI GAGTC 4 cut(s) 137, 710, 3433, 4134
MmeI TCCRAC 8 cut(s) 109, 161, 1317, 1416, 1616, 1747, 3264, 4065
Mox20I TGGCCA 4 cut(s) 602, 2612, 2683, 2740
Mph1103I ATGCAT 2 cut(s) 440, 3380
MroXI GAANNNNTTC 4 cut(s) 303, 546, 2123, 2880
MscI TGGCCA 4 cut(s) 602, 2612, 2683, 2740
MslI CAYNNNNRTG 4 cut(s) 403, 1055, 2585, 4338
Msp20I TGGCCA 4 cut(s) 602, 2612, 2683, 2740
MspA1I CMGCKG 3 cut(s) 1124, 1445, 2231
MspCI CTTAAG 2 cut(s) 3032, 3071
MspI CCGG 5 cut(s) 1659, 2716, 3276, 4169, 4202
MspR9I CCNGG 5 cut(s) 177, 771, 820, 1054, 3735
MunI CAATTG 1 cut(s) 2439
Mva1269I GAATGC 3 cut(s) 440, 1576, 2204
MvaI CCWGG 5 cut(s) 177, 771, 820, 1054, 3735
MvnI CGCG 1 cut(s) 95
MwoI GCNNNNNNNGC 7 cut(s) 2055, 2225, 2797, 2928, 3989, 4152, 4393
NcoI CCATGG 2 cut(s) 1695, 3559
NdeI CATATG 1 cut(s) 3968
NlaIV GGNNCC 6 cut(s) 407, 483, 1440, 2072, 2720, 3602
NmeAIII GCCGAG 1 cut(s) 1117
NmuCI GTSAC 8 cut(s) 1021, 1057, 1732, 2238, 2864, 3124, 3402, 3487
NsiI ATGCAT 2 cut(s) 440, 3380
NspI RCATGY 4 cut(s) 587, 1936, 3327, 3992
NspV TTCGAA 2 cut(s) 2343, 2602
OliI CACNNNNGTG 1 cut(s) 1055
PaeI GCATGC 3 cut(s) 1936, 3327, 3992
PaeR7I CTCGAG 1 cut(s) 1975
PagI TCATGA 7 cut(s) 559, 1036, 1917, 2127, 2152, 2752, 2778
PasI CCCWGGG 1 cut(s) 819
PciI ACATGT 1 cut(s) 583
PctI GAATGC 3 cut(s) 440, 1576, 2204
PdmI GAANNNNTTC 4 cut(s) 303, 546, 2123, 2880
PfeI GAWTC 6 cut(s) 1310, 1563, 1892, 2111, 2156, 3046
PflFI GACNNNGTC 1 cut(s) 4124
PflMI CCANNNNNTGG 3 cut(s) 770, 962, 2065
PleI GAGTC 4 cut(s) 136, 709, 3433, 4133
PpsI GAGTC 4 cut(s) 136, 709, 3433, 4133
Ppu21I YACGTR 2 cut(s) 2864, 4273
PscI ACATGT 1 cut(s) 583
PsiI TTATAA 3 cut(s) 200, 1431, 1940
Psp124BI GAGCTC 1 cut(s) 2829
Psp6I CCWGG 5 cut(s) 175, 769, 818, 1052, 3733
PspFI CCCAGC 3 cut(s) 753, 763, 1897
PspGI CCWGG 5 cut(s) 175, 769, 818, 1052, 3733
PspN4I GGNNCC 6 cut(s) 407, 483, 1440, 2072, 2720, 3602
PspPI GGNCC 2 cut(s) 739, 3977
PstI CTGCAG 1 cut(s) 110
PstNI CAGNNNCTG 1 cut(s) 770
PsuI RGATCY 7 cut(s) 481, 1132, 1438, 2082, 3136, 3881, 4195
PsyI GACNNNGTC 1 cut(s) 4124
PvuII CAGCTG 2 cut(s) 1124, 2231
RseI CAYNNNNRTG 4 cut(s) 403, 1055, 2585, 4338
SacI GAGCTC 1 cut(s) 2829
SalI GTCGAC 2 cut(s) 130, 703
Sau96I GGNCC 2 cut(s) 739, 3977
ScaI AGTACT 1 cut(s) 1644
SchI GAGTC 4 cut(s) 137, 710, 3433, 4134
ScrFI CCNGG 5 cut(s) 177, 771, 820, 1054, 3735
SduI GDGCHC 6 cut(s) 834, 2518, 2829, 3603, 4209, 4253
SfcI CTRYAG 2 cut(s) 106, 696
Sfr274I CTCGAG 1 cut(s) 1975
SfuI TTCGAA 2 cut(s) 2343, 2602
SinI GGWCC 2 cut(s) 739, 3977
SlaI CTCGAG 1 cut(s) 1975
SmiMI CAYNNNNRTG 4 cut(s) 403, 1055, 2585, 4338
SmlI CTYRAG 6 cut(s) 1975, 3032, 3041, 3071, 3704, 3916
SmoI CTYRAG 6 cut(s) 1975, 3032, 3041, 3071, 3704, 3916
SpeI ACTAGT 2 cut(s) 685, 3958
SphI GCATGC 3 cut(s) 1936, 3327, 3992
SspI AATATT 3 cut(s) 1628, 2810, 3910
SstI GAGCTC 1 cut(s) 2829
StyD4I CCNGG 5 cut(s) 175, 769, 818, 1052, 3733
StyI CCWWGG 4 cut(s) 18, 1695, 1741, 3559
TaaI ACNGT 6 cut(s) 286, 1276, 1738, 2164, 2564, 3678
TaiI ACGT 8 cut(s) 81, 495, 663, 2138, 2361, 2866, 3126, 4275
TaqII GACCGA 1 cut(s) 756
TatI WGTACW 4 cut(s) 532, 1642, 3345, 3513
TfiI GAWTC 6 cut(s) 1310, 1563, 1892, 2111, 2156, 3046
TscAI CASTG 7 cut(s) 1397, 1538, 1741, 3133, 3582, 4112, 4228
TseFI GTSAC 8 cut(s) 1021, 1057, 1732, 2238, 2864, 3124, 3402, 3487
Tsp45I GTSAC 8 cut(s) 1021, 1057, 1732, 2238, 2864, 3124, 3402, 3487
TspGWI ACGGA 4 cut(s) 149, 1610, 3007, 4087
TspRI CASTG 7 cut(s) 1397, 1538, 1741, 3133, 3582, 4112, 4228
Tth111I GACNNNGTC 1 cut(s) 4124
Van91I CCANNNNNTGG 3 cut(s) 770, 962, 2065
Vha464I CTTAAG 2 cut(s) 3032, 3071
VpaK11BI GGWCC 2 cut(s) 739, 3977
XbaI TCTAGA 3 cut(s) 2546, 3856, 3949
XceI RCATGY 4 cut(s) 587, 1936, 3327, 3992
XhoI CTCGAG 1 cut(s) 1975
XmiI GTMKAC 5 cut(s) 131, 695, 704, 2860, 3944
XmnI GAANNNNTTC 4 cut(s) 303, 546, 2123, 2880
ZraI GACGTC 2 cut(s) 2136, 3124
ZrmI AGTACT 1 cut(s) 1644
Zsp2I ATGCAT 2 cut(s) 440, 3380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.