FvH4_7g13640

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000085
Physical Location & Seq
Forward (+)
12218105 .. 12221635
3531 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g13640.t1

Sequence Viewer

Length: 3318 bp
ATGGCCTCAACCTCCCACACAGCCTCAGCATCGCTTCCTTCATCGCCTCGTAGTTGGAAGTATGATGTGTTTTTGAGTTTTAGGGGTGTCGATACTCGGAAAGGTATTACATTTGAACTATTTGATCGTCTGCAGCACAAAAGAGGAATTAAAACATTTATGGATGACCAAGACCTTCCAGCAGGGGCTGCTATTTCCCCGACTCTCCTAGCAGCAATTGAAGAATCAAGGTTTGCAATTGTTGTTTTCTGCCGGAACTATGCTTCTTCTACTTGGTGCTTGGAGGAACTTGTAAAGATTTGTCAATGCATGGAAGACGAAAACAGAATTCTTCCACTTTTCTATCATGTTGATCCTAGTGACATTCGATATCAGAAAAGGAGTTTCGAAGAAGCTTTCACTAAGCATGTAAAATCTAACAGACACGAATCAAAGAAGGTGCAGGAGTGGAGAGATGCTTTAGCAAAAGTGGCAAATTCTTCTGGGTGGCATACACAGAATTACAAGACTGAAAGAGAACTTGCCGATGCCATTGTGGACTTTGTGTGCAGTATAGTACAACCGATTGAAATCATCATGGGAGATTTTGAAGTATTCGAAGCAACAAGGGAAGCCATGGAGAAGGTTATGAAAGCACTTGAAGATGACAAGGTCTCAACTGTCGGCGTGTATGGAATGGGAGGGGTTGGGAAGACAACCATGGTAAAACATGTGGGTGATCAAGCCCGCAAAAATGGGATTTTTCATTATGTGATCATGGCTGTCATGTCCCAAAGCCCTGACTTGACAGAAATTCAGCACACATTGGCAGAGCTTCTGGGGATCGATTTACAAGGGAAGTCACAAAAAGGAAGAGCCTCTAGATTGAACAAAGAGATAATGAGGAAAAACAAAATCCTTATAATCTTGGACGACATATGGGAGAGAATAGAATTGTCAAGCATAGGGATTCCCAGCTACAAGGAACTTCAAAAGTGTAATTCCAAAGTCCTAGTCACAACAAGGATACAGAATGTTTGTCATGCCATGGAGTGCCAAGAAATGATCACCCTCAATATCCTCTCGAAAGAGGATTCTTGGACCTTATTTATGAGAAATGCTAGAAGCTCTTTTGAAACAACCATTTTCAGGGATGTAGCGAGGAAGGTGGCTAGAGAATGCTGTGGTCTACCAATTGCATTGATAGCAGTTGCAAAGGCCCTTGGAGATAAAGACCTGCGGGAATGGAAAAAGGCAGCTCAAAGACTAAACAAGTCGCAAACTGCCAACCTTGACGATAAGGGAGATGCATCCCAATGTATAAAGTTAAGCTATGATTACTTGAAAGATGAGGATTGCAAGACATGCTTCTTGGTTTGTAGCCTATTCCCTGAAGACTATGACATCCCAATAGAAGACTTGTTCAGGTATGCAATCGGGAAAGGTGTGTTTCGAGATGCTGACACAATCGAAGAAGCTAGAGAAACACTACATATGGTGGTCACATACCTTAAGAATTCAAGCTTGCTTTTAAATAGTGACAAAGAACTATGTGTAAAGATGCATGATGTCATTCGGGATACGACCATGAAAATTGCACTATCTGAAGACGGTCACGGGTTTTTGGTAAAAGCTGGCTGCGGTTTAGAGGATTGGCCCCATCAATTACATGAAGGCTACTCCGCGGTCTCACTGATTGGGAATGAAATCCGCACAGTACCAGAAGAATTGGTATGTCCAAAACTCCAGGTTTTATTACTACAAGATAATTGCTATATAGAAAAGTTCCCAGGTACCTTTTTTCATAGTTCCAATGAATTAAGGGTATTAAATCTTAGCAACACTTGCATTTTATCACTACCCCACTCATTCAGTCTTCTAGCCAACCTCCAAGTCTTGCATTTAGATGATTTGAAGATCATAACCGACATTTCCATGCTCGGAAAACTGAAAAAACTTGAGATCCTTAGTATGAGAGAATCAAACATTGGTGAATTCCCAAAAGAAATAGGAGAGTTGACCAATCTAAGGATGTTGGATTTGACAGGAGGACATATTGTCACAATACCATCAAAAGTGTTTTCAAAGTTGCATAGACTTGAAGAACTTTACATGCAATGTGGGTTTTGGGACTGGGGGAGTGAAATTGAAGGAGGAGGAGGAGAAACCAATGCTGGCTTTGATGAGTTGACTAGCTTGCCACTTTTAAGAGTTCTGAAAGTTTGGATCTCCCATGCAAATTGTATCCCTGAAACTGTTAAACTCATCCCGAATTGGGTTCAGTTTGATATATGTATTGGAAGAAACGATGAGATTGTAGTCTCTCAGTATTGTTATAATTCAGTGTGCTTGACACTTGACACAACCATCACCACATTACCGGATTGGTTTGTCTTCGTAGTGATAAACAAAGCAGAGAAGCTAGAGTGGATAGAGTGCAAAGGGTTGATTGATTTTTTTGTGGAATATGAGAGTGGGAGGTTAGACAGACTGGATTTTTGTGTGGAATATGAGCATGAGAGGTTAGACAGACTGAAGTATCTCTCTATAACTGGTTGCGATGAGGACTCAAAAGAGTTGATGAACACAACTACATGGGTTCCGAAAGAGCCTGTGTTTGAGAACTTGAAAGACTTGCATCTCAGTCAAGTAAATTTCATGAAAGAGTTATGTCTTGGTGATTTACCTCATGGATCTCTATTCAGCCTGAAGTTACTTGAGGTTGACTCTTGTTATAACTGGGGGAACATACTTTTACCATCAAAATTGTCGCAGAGAGTATCAAATCTGGAAACGCTAATTTGTAATGGTGTTGATGGAATCGAATATGTATTTGGATGCGATAGCTTTAGGCTGGAAAATTCAAAATTGAGAGACCTGTCATTGAGGAGTTTAGATGCAGTAAGAAGCATATGTGATGGTCCTGCTCCACCTGCAATGTTCCAGACTCTTAAAATTTTGTCCATTGAACAATGCAGCTTTGTGGGAAGTCTTTTCTCCTATGAGGCAGCTCAATATCTTTTTCAATTGGAAGACCTTTCGGTTGGCAGTTGCCTTTTATTGGAAAGAATTATTGAAGCAAACAAGGAGACAATGAACACGAGGAAGACCGTTTTTCCAAAATTGAAGAACTTAGTTTTGATGAGACTTCCTATGCTGTACCGTGGATGTGCTACTATTGATTTTGAGTGTCCCTTACTAGAATGCTTGTATTTGAAGGACTGCTCCCAGTTTTCATCTTCAACCTCTGCTTCTGACTTCCACAGTAGGAAAAATGTCAAGTTCAATGATGAGCAACACTACCTGTCTCTATTGGAGAGAATGTGGGGAGACACATAA

Protein Analysis

1106

Amino Acids

125.77

Weight (kDa)

5.44

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 20 - 182 7.6e-47 TIR domain
TIR_2 PF13676 23 - 122 9.9e-13 TIR domain
NB-ARC PF00931 204 - 366 4.4e-31 NB-ARC domain
LRR_8 PF13855 573 - 629 1.2e-07 Leucine rich repeat
LRR_14 PF23598 637 - 843 3.3e-10 Leucine-rich repeat region
LRR_RPS2 PF23247 868 - 966 2.8e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 951 - 1047 3.1e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 902, 2316, 2715
AarI CACCTGC 1 cut(s) 2920
Acc36I ACCTGC 2 cut(s) 1224, 2920
Acc65I GGTACC 1 cut(s) 1772
AccB1I GGYRCC 1 cut(s) 1772
AccI GTMKAC 1 cut(s) 1168
AccII CGCG 1 cut(s) 1664
AciI CCGC 6 cut(s) 727, 1219, 1620, 1662, 1664, 1690
AclWI GGATC 5 cut(s) 347, 830, 1936, 2213, 2680
AcsI RAATTY 8 cut(s) 327, 475, 792, 1495, 1973, 2632, 2839, 2934
AcuI CTGAAG 4 cut(s) 1392, 1605, 2534, 2708
AfaI GTAC 4 cut(s) 558, 1698, 1774, 3140
AfiI CCNNNNNNNGG 4 cut(s) 1128, 2007, 2254, 3040
AflII CTTAAG 1 cut(s) 1490
AflIII ACRYGT 1 cut(s) 709
AhdI GACNNNNNGTC 1 cut(s) 2036
AjnI CCWGG 2 cut(s) 1725, 1768
AjuI GAANNNNNNNTTGG 4 cut(s) 1950, 1982, 2796, 2828
Alw26I GTCTC 8 cut(s) 658, 1672, 2305, 2847, 3062, 3118, 3291, 3303
AlwI GGATC 5 cut(s) 347, 830, 1936, 2213, 2680
AlwNI CAGNNNCTG 1 cut(s) 188
AoxI GGCC 3 cut(s) 3, 1197, 1634
ApeKI GCWGC 7 cut(s) 133, 188, 212, 1235, 1617, 2955, 2987
ApoI RAATTY 8 cut(s) 327, 475, 792, 1495, 1973, 2632, 2839, 2934
ArsI GACNNNNNNTTYG 4 cut(s) 978, 1010, 2460, 2492
Asp718I GGTACC 1 cut(s) 1772
AspS9I GGNCC 4 cut(s) 1080, 1198, 1635, 2900
AsuHPI GGTGA 5 cut(s) 728, 1039, 1982, 2341, 2669
AsuII TTCGAA 2 cut(s) 387, 597
AvaII GGWCC 2 cut(s) 1080, 2900
BaeI ACNNNNGTAYC 2 cut(s) 2501, 2534
BanI GGYRCC 1 cut(s) 1772
BauI CACGAG 1 cut(s) 3079
BbsI GAAGAC 9 cut(s) 321, 698, 1380, 1401, 1593, 1847, 2365, 3018, 3092
BbvCI CCTCAGC 1 cut(s) 25
BbvI GCAGC 7 cut(s) 145, 175, 224, 1247, 1604, 2967, 2999
BccI CCATC 6 cut(s) 1647, 2056, 2354, 2746, 2789, 2891
BciT130I CCWGG 2 cut(s) 1727, 1770
BciVI GTATCC 3 cut(s) 999, 1552, 2234
BclI TGATCA 3 cut(s) 718, 753, 1044
BcoDI GTCTC 8 cut(s) 658, 1672, 2305, 2847, 3062, 3118, 3291, 3303
BfmI CTRYAG 1 cut(s) 131
BfrI CTTAAG 1 cut(s) 1490
BfuAI ACCTGC 2 cut(s) 1224, 2920
BfuI GTATCC 3 cut(s) 999, 1552, 2234
BisI GCNGC 7 cut(s) 134, 189, 213, 1236, 1618, 2956, 2988
BlsI GCNGC 7 cut(s) 135, 190, 214, 1237, 1619, 2957, 2989
Bme1390I CCNGG 2 cut(s) 1727, 1770
Bme18I GGWCC 2 cut(s) 1080, 2900
BmeRI GACNNNNNGTC 1 cut(s) 2036
BmgT120I GGNCC 4 cut(s) 1080, 1198, 1635, 2900
BmiI GGNNCC 3 cut(s) 1637, 1774, 2580
BmrFI CCNGG 2 cut(s) 1727, 1770
BmrI ACTGGG 3 cut(s) 2122, 2728, 3202
BmuI ACTGGG 3 cut(s) 2122, 2728, 3202
BpiI GAAGAC 9 cut(s) 321, 698, 1380, 1401, 1593, 1847, 2365, 3018, 3092
BplI GAGNNNNNCTC 2 cut(s) 2690, 2722
BpmI CTGGAG 1 cut(s) 1709
Bpu10I CCTNAGC 1 cut(s) 25
Bpu14I TTCGAA 2 cut(s) 387, 597
BpuEI CTTGAG 2 cut(s) 1958, 2717
Bsa29I ATCGAT 1 cut(s) 825
BsaBI GATNNNNATC 1 cut(s) 569
BsaI GGTCTC 3 cut(s) 658, 1672, 2847
BsaJI CCNNGG 7 cut(s) 615, 699, 1026, 1201, 1662, 1768, 3142
BsaWI WCCGGW 1 cut(s) 2359
BsaXI ACNNNNNCTCC 2 cut(s) 3287, 3317
Bsc4I CCNNNNNNNGG 4 cut(s) 1128, 2007, 2254, 3040
Bse1I ACTGG 5 cut(s) 2117, 2475, 2536, 2723, 3208
Bse3DI GCAATG 2 cut(s) 2102, 2922
Bse8I GATNNNNATC 1 cut(s) 569
BseBI CCWGG 2 cut(s) 1727, 1770
BseCI ATCGAT 1 cut(s) 825
BseDI CCNNGG 7 cut(s) 615, 699, 1026, 1201, 1662, 1768, 3142
BseGI GGATG 8 cut(s) 169, 1138, 1289, 1383, 2016, 2244, 2822, 3152
BseJI GATNNNNATC 1 cut(s) 569
BseLI CCNNNNNNNGG 4 cut(s) 1128, 2007, 2254, 3040
BseMI GCAATG 2 cut(s) 2102, 2922
BseMII CTCAG 3 cut(s) 39, 2318, 2635
BseNI ACTGG 5 cut(s) 2117, 2475, 2536, 2723, 3208
BseRI GAGGAG 4 cut(s) 2148, 2151, 2154, 2881
BseXI GCAGC 7 cut(s) 145, 175, 224, 1247, 1604, 2967, 2999
BseYI CCCAGC 1 cut(s) 953
BsgI GTGCAG 2 cut(s) 461, 568
Bsh1236I CGCG 1 cut(s) 1664
BshFI GGCC 3 cut(s) 5, 1199, 1636
BshNI GGYRCC 1 cut(s) 1772
BshVI ATCGAT 1 cut(s) 825
BsiSI CCGG 2 cut(s) 253, 2360
BslFI GGGAC 3 cut(s) 754, 2123, 3156
BslI CCNNNNNNNGG 4 cut(s) 1128, 2007, 2254, 3040
BsmAI GTCTC 8 cut(s) 658, 1672, 2305, 2847, 3062, 3118, 3291, 3303
BsmFI GGGAC 3 cut(s) 754, 2123, 3156
BsmI GAATGC 2 cut(s) 1163, 3188
BsnI GGCC 3 cut(s) 5, 1199, 1636
Bso31I GGTCTC 3 cut(s) 658, 1672, 2847
Bsp119I TTCGAA 2 cut(s) 387, 597
Bsp19I CCATGG 3 cut(s) 615, 699, 1026
BspACI CCGC 6 cut(s) 727, 1219, 1620, 1662, 1664, 1690
BspANI GGCC 3 cut(s) 5, 1199, 1636
BspCNI CTCAG 3 cut(s) 38, 2317, 2634
BspDI ATCGAT 1 cut(s) 825
BspFNI CGCG 1 cut(s) 1664
BspHI TCATGA 1 cut(s) 2637
BspLI GGNNCC 3 cut(s) 1637, 1774, 2580
BspMAI CTGCAG 1 cut(s) 135
BspMI ACCTGC 2 cut(s) 1224, 2920
BspPI GGATC 5 cut(s) 347, 830, 1936, 2213, 2680
BspQI GCTCTTC 1 cut(s) 847
BspT104I TTCGAA 2 cut(s) 387, 597
BspT107I GGYRCC 1 cut(s) 1772
BspTI CTTAAG 1 cut(s) 1490
BspTNI GGTCTC 3 cut(s) 658, 1672, 2847
BsrDI GCAATG 2 cut(s) 2102, 2922
BsrI ACTGG 5 cut(s) 2117, 2475, 2536, 2723, 3208
BssECI CCNNGG 7 cut(s) 615, 699, 1026, 1201, 1662, 1768, 3142
BssSI CACGAG 1 cut(s) 3079
BssT1I CCWWGG 4 cut(s) 615, 699, 1026, 1201
Bst2BI CACGAG 1 cut(s) 3079
Bst2UI CCWGG 2 cut(s) 1727, 1770
Bst4CI ACNGT 7 cut(s) 661, 1592, 1696, 2236, 3091, 3143, 3245
Bst6I CTCTTC 1 cut(s) 847
BstAFI CTTAAG 1 cut(s) 1490
BstAPI GCANNNNNTGC 3 cut(s) 188, 1344, 1824
BstBI TTCGAA 2 cut(s) 387, 597
BstC8I GCNNGC 5 cut(s) 727, 1505, 1615, 2155, 2177
BstDEI CTNAG 8 cut(s) 25, 402, 1814, 1946, 2006, 2304, 2621, 3112
BstDSI CCRYGG 5 cut(s) 615, 699, 1026, 1662, 3142
BstF5I GGATG 8 cut(s) 169, 1138, 1289, 1383, 2016, 2244, 2822, 3152
BstFNI CGCG 1 cut(s) 1664
BstMAI GTCTC 8 cut(s) 658, 1672, 2305, 2847, 3062, 3118, 3291, 3303
BstMWI GCNNNNNNNGC 6 cut(s) 188, 470, 1184, 1344, 1824, 2912
BstNI CCWGG 2 cut(s) 1727, 1770
BstNSI RCATGY 4 cut(s) 410, 713, 1347, 2095
BstSCI CCNGG 2 cut(s) 1725, 1768
BstSFI CTRYAG 1 cut(s) 131
BstUI CGCG 1 cut(s) 1664
BstV1I GCAGC 7 cut(s) 145, 175, 224, 1247, 1604, 2967, 2999
BstV2I GAAGAC 9 cut(s) 321, 698, 1380, 1401, 1593, 1847, 2365, 3018, 3092
BstX2I RGATCY 3 cut(s) 1941, 2205, 2672
BstYI RGATCY 3 cut(s) 1941, 2205, 2672
Bsu15I ATCGAT 1 cut(s) 825
BsuI GTATCC 3 cut(s) 999, 1552, 2234
BsuRI GGCC 3 cut(s) 5, 1199, 1636
BsuTUI ATCGAT 1 cut(s) 825
BtgI CCRYGG 5 cut(s) 615, 699, 1026, 1662, 3142
BtgZI GCGATG 3 cut(s) 15, 27, 2553
BtsCI GGATG 8 cut(s) 169, 1138, 1289, 1383, 2016, 2244, 2822, 3152
BtsIMutI CAGTG 2 cut(s) 1670, 2328
BveI ACCTGC 2 cut(s) 1224, 2920
Cac8I GCNNGC 5 cut(s) 727, 1505, 1615, 2155, 2177
CaiI CAGNNNCTG 1 cut(s) 188
CciI TCATGA 1 cut(s) 2637
Cfr13I GGNCC 4 cut(s) 1080, 1198, 1635, 2900
Cfr42I CCGCGG 1 cut(s) 1665
ClaI ATCGAT 1 cut(s) 825
Csp6I GTAC 4 cut(s) 557, 1697, 1773, 3139
CviQI GTAC 4 cut(s) 557, 1697, 1773, 3139
DdeI CTNAG 8 cut(s) 25, 402, 1814, 1946, 2006, 2304, 2621, 3112
DraI TTTAAA 1 cut(s) 1512
DriI GACNNNNNGTC 1 cut(s) 2036
Eam1104I CTCTTC 1 cut(s) 847
Eam1105I GACNNNNNGTC 1 cut(s) 2036
EarI CTCTTC 1 cut(s) 847
Eco130I CCWWGG 4 cut(s) 615, 699, 1026, 1201
Eco31I GGTCTC 3 cut(s) 658, 1672, 2847
Eco32I GATATC 1 cut(s) 371
Eco47I GGWCC 2 cut(s) 1080, 2900
Eco57I CTGAAG 4 cut(s) 1392, 1605, 2534, 2708
EcoO109I RGGNCCY 1 cut(s) 1198
EcoRI GAATTC 3 cut(s) 327, 1495, 1973
EcoRII CCWGG 2 cut(s) 1725, 1768
EcoRV GATATC 1 cut(s) 371
EcoT14I CCWWGG 4 cut(s) 615, 699, 1026, 1201
EcoT22I ATGCAT 3 cut(s) 311, 1291, 1545
ErhI CCWWGG 4 cut(s) 615, 699, 1026, 1201
FalI AAGNNNNNCTT 2 cut(s) 1331, 1363
FaqI GGGAC 3 cut(s) 754, 2123, 3156
FauI CCCGC 2 cut(s) 734, 1212
FauNDI CATATG 3 cut(s) 917, 1473, 2891
FbaI TGATCA 3 cut(s) 718, 753, 1044
FblI GTMKAC 1 cut(s) 1168
Fnu4HI GCNGC 7 cut(s) 134, 189, 213, 1236, 1618, 2956, 2988
FokI GGATG 8 cut(s) 176, 1145, 1276, 1370, 2023, 2231, 2829, 3159
Fsp4HI GCNGC 7 cut(s) 134, 189, 213, 1236, 1618, 2956, 2988
GluI GCNGC 7 cut(s) 134, 189, 213, 1236, 1618, 2956, 2988
GsaI CCCAGC 1 cut(s) 957
GsuI CTGGAG 1 cut(s) 1709
HaeIII GGCC 3 cut(s) 5, 1199, 1636
HapII CCGG 2 cut(s) 253, 2360
HincII GTYRAC 3 cut(s) 1998, 2169, 2704
HindII GTYRAC 3 cut(s) 1998, 2169, 2704
HindIII AAGCTT 2 cut(s) 393, 1501
HpaII CCGG 2 cut(s) 253, 2360
HphI GGTGA 5 cut(s) 728, 1039, 1982, 2341, 2669
Hpy166II GTNNAC 5 cut(s) 538, 1169, 1998, 2169, 2704
Hpy188I TCNGA 7 cut(s) 99, 375, 1585, 1922, 2196, 2583, 3235
Hpy188III TCNNGA 9 cut(s) 861, 1063, 1417, 1433, 1556, 2248, 2638, 2768, 2923
Hpy8I GTNNAC 5 cut(s) 538, 1169, 1998, 2169, 2704
HpyAV CCTTC 8 cut(s) 48, 185, 430, 616, 1138, 1646, 2123, 3190
HpyCH4III ACNGT 7 cut(s) 661, 1592, 1696, 2236, 3091, 3143, 3245
HpyF10VI GCNNNNNNNGC 6 cut(s) 188, 470, 1184, 1344, 1824, 2912
HpyF3I CTNAG 8 cut(s) 25, 402, 1814, 1946, 2006, 2304, 2621, 3112
KpnI GGTACC 1 cut(s) 1776
Ksp22I TGATCA 3 cut(s) 718, 753, 1044
KspI CCGCGG 1 cut(s) 1665
LguI GCTCTTC 1 cut(s) 847
LmnI GCTCC 2 cut(s) 2911, 3209
Lsp1109I GCAGC 7 cut(s) 145, 175, 224, 1247, 1604, 2967, 2999
MaeIII GTNAC 8 cut(s) 359, 840, 994, 1480, 1517, 1592, 2038, 2691
MfeI CAATTG 4 cut(s) 216, 237, 1173, 3005
MflI RGATCY 3 cut(s) 1941, 2205, 2672
MlyI GAGTC 4 cut(s) 196, 2540, 2699, 2920
MmeI TCCRAC 2 cut(s) 35, 1995
Mph1103I ATGCAT 3 cut(s) 311, 1291, 1545
MseI TTAA 9 cut(s) 150, 1307, 1491, 1511, 1799, 1808, 2186, 2238, 2931
MslI CAYNNNNRTG 4 cut(s) 714, 1294, 1884, 1913
MspA1I CMGCKG 1 cut(s) 1664
MspCI CTTAAG 1 cut(s) 1490
MspI CCGG 2 cut(s) 253, 2360
MspR9I CCNGG 2 cut(s) 1727, 1770
MunI CAATTG 4 cut(s) 216, 237, 1173, 3005
Mva1269I GAATGC 2 cut(s) 1163, 3188
MvaI CCWGG 2 cut(s) 1727, 1770
MvnI CGCG 1 cut(s) 1664
MwoI GCNNNNNNNGC 6 cut(s) 188, 470, 1184, 1344, 1824, 2912
NcoI CCATGG 3 cut(s) 615, 699, 1026
NdeI CATATG 3 cut(s) 917, 1473, 2891
NlaIV GGNNCC 3 cut(s) 1637, 1774, 2580
NmuCI GTSAC 7 cut(s) 359, 840, 994, 1480, 1517, 1592, 2038
NsiI ATGCAT 3 cut(s) 311, 1291, 1545
NspI RCATGY 4 cut(s) 410, 713, 1347, 2095
NspV TTCGAA 2 cut(s) 387, 597
PagI TCATGA 1 cut(s) 2637
PaqCI CACCTGC 1 cut(s) 2920
PciI ACATGT 1 cut(s) 709
PciSI GCTCTTC 1 cut(s) 847
PctI GAATGC 2 cut(s) 1163, 3188
PfeI GAWTC 6 cut(s) 224, 428, 949, 1073, 1958, 2799
PflFI GACNNNGTC 1 cut(s) 650
PkrI GCNGC 7 cut(s) 135, 190, 214, 1237, 1619, 2957, 2989
PleI GAGTC 4 cut(s) 196, 2540, 2699, 2920
PpsI GAGTC 4 cut(s) 196, 2540, 2699, 2920
PscI ACATGT 1 cut(s) 709
PsiI TTATAA 3 cut(s) 902, 2316, 2715
Psp6I CCWGG 2 cut(s) 1725, 1768
PspFI CCCAGC 1 cut(s) 953
PspGI CCWGG 2 cut(s) 1725, 1768
PspN4I GGNNCC 3 cut(s) 1637, 1774, 2580
PspPI GGNCC 4 cut(s) 1080, 1198, 1635, 2900
PstI CTGCAG 1 cut(s) 135
PstNI CAGNNNCTG 1 cut(s) 188
PsuI RGATCY 3 cut(s) 1941, 2205, 2672
PsyI GACNNNGTC 1 cut(s) 650
RsaI GTAC 4 cut(s) 558, 1698, 1774, 3140
RsaNI GTAC 4 cut(s) 557, 1697, 1773, 3139
RseI CAYNNNNRTG 4 cut(s) 714, 1294, 1884, 1913
SacII CCGCGG 1 cut(s) 1665
SapI GCTCTTC 1 cut(s) 847
SaqAI TTAA 9 cut(s) 150, 1307, 1491, 1511, 1799, 1808, 2186, 2238, 2931
SatI GCNGC 7 cut(s) 134, 189, 213, 1236, 1618, 2956, 2988
Sau96I GGNCC 4 cut(s) 1080, 1198, 1635, 2900
SchI GAGTC 4 cut(s) 196, 2540, 2699, 2920
ScrFI CCNGG 2 cut(s) 1727, 1770
SfcI CTRYAG 1 cut(s) 131
Sfr303I CCGCGG 1 cut(s) 1665
SfuI TTCGAA 2 cut(s) 387, 597
SgrBI CCGCGG 1 cut(s) 1665
SinI GGWCC 2 cut(s) 1080, 2900
SmiMI CAYNNNNRTG 4 cut(s) 714, 1294, 1884, 1913
SmlI CTYRAG 3 cut(s) 1490, 1937, 2696
SmoI CTYRAG 3 cut(s) 1490, 1937, 2696
SsiI CCGC 6 cut(s) 727, 1219, 1620, 1662, 1664, 1690
StyD4I CCNGG 2 cut(s) 1725, 1768
StyI CCWWGG 4 cut(s) 615, 699, 1026, 1201
TaaI ACNGT 7 cut(s) 661, 1592, 1696, 2236, 3091, 3143, 3245
TaqI TCGA 9 cut(s) 90, 367, 387, 597, 825, 1064, 1432, 1449, 2802
TatI WGTACW 1 cut(s) 556
TfiI GAWTC 6 cut(s) 224, 428, 949, 1073, 1958, 2799
Tru1I TTAA 9 cut(s) 150, 1307, 1491, 1511, 1799, 1808, 2186, 2238, 2931
Tru9I TTAA 9 cut(s) 150, 1307, 1491, 1511, 1799, 1808, 2186, 2238, 2931
TscAI CASTG 2 cut(s) 1677, 2328
TseFI GTSAC 7 cut(s) 359, 840, 994, 1480, 1517, 1592, 2038
TseI GCWGC 7 cut(s) 133, 188, 212, 1235, 1617, 2955, 2987
Tsp45I GTSAC 7 cut(s) 359, 840, 994, 1480, 1517, 1592, 2038
TspRI CASTG 2 cut(s) 1677, 2328
Tth111I GACNNNGTC 1 cut(s) 650
Vha464I CTTAAG 1 cut(s) 1490
VpaK11BI GGWCC 2 cut(s) 1080, 2900
XapI RAATTY 8 cut(s) 327, 475, 792, 1495, 1973, 2632, 2839, 2934
XbaI TCTAGA 1 cut(s) 860
XceI RCATGY 4 cut(s) 410, 713, 1347, 2095
XmiI GTMKAC 1 cut(s) 1168
Zsp2I ATGCAT 3 cut(s) 311, 1291, 1545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.