Rroxscaffold_1G00011370

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000085
Physical Location & Seq
Reverse (-)
14352038 .. 14359302
7265 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00011370.1

Sequence Viewer

Length: 3327 bp
ATGGCCTCGAGCAGCCAAAGAGCCTCTGAATCTTTTCCTTCAGCCAAGTCAACGGTTCTCAAGTGGAAGTATGATGTGTTTTTGAGTTTTAGGGGTGAAGACATTCGGAACAATTTTCTCTCTTATTTATACCATGAATTGCAAATCATCAGAAGGATTAATACATTCAAGGATGACGAAGAGCTTGAAGTCGGGGATCCTATTTCTCCAAGTCTCCTAAAAGCAATCGATGAATCAAGGTTTGCTATTATTGTTCTGTCGCCAAACTATGCTTCTTCTCCATGGTGTTTGGAGGAACTTGCAAAAATTGTTCATTGCATGGAAGACACCAGGAGAATTCTGCCACTTTTTTATCATGTGGAACCAGCTGATGTACGACATCAAAAAAATAGTTTTGAAGTAGCCTTCACTAAGCATGAAGAAAAGTCAAGGCATGACACAGAGAAGGTGAAGCAGTGGAGAGATGCTTTAAAAAAAGTGGCTAATTTATCTGGATGGCATACGCAGAGATATCAGACAGAAAGAGAACTTGTTGAAGCCATTGCAGATTATGTGTGCACTAAGGTACGACCAATTGAAAAGGAGTTCTCAAAGTCCACTGGAGATTTTGAAGCATTTGAAGCAACAAGGCAAGCCATGGATGAGGTCTGGAAGGCACTAAAAGATGATAAGGTCACTGCCATTGCGGTGTATGGAATGGGAGGTGTTGGGAAGACAACTATGGTAGAATATGTCGGTGTACAAGCTCAAAATAGTGGGATTTTCCATCATGTGATTATGGTTGTTATATCCCAAAGGCCCAACTTGAGAAATATCCAAGGCGCATTGGCAGATCTGCTGGGCCTTGAATTGAAGGAGGAGACAGACATTGGAAGAGCCAATAGACTGAAGAAGGAGATAATGAGAAGAAATAAGATCCTTTTAATCTTGGATGACATTTGGGAGAGAATAGACTTGTCAAGCATAGGGATCCCCAGTTACGACAAGCTTCAGAAATGCAATTCAAAAGTCCTACTCACCACAAGGAGATTGAATGTTTGTCATTCTATGGAAACACAACCAAGCATTCCTCTCAATATCTTATCAGAAGAAGATTCTTGGAACTTGTTTGTGAAGAAAGCAAGAAAGTCTATCGAAAAATCAACTGAATTCTATGATGTAGCGAGGAAGGTAGCCAGTGAGTGTGCTGGTCTCCCTGTTGCACTGAAAGCAGTTGCGAGGGCACTTGGAGACAAAGATTTTGAAGAATGGAAAGAAGCAGCTCGAAAGCTGGAAATGTCTCAACGTGCTAGCCTCGATGATGATGGAGATGTGTTTAAATGTATAAAGTTAAGTTATGATTACTTGGACAGTATTGATGCCAAGTCATGCTTCTTGCTGTGCTGCCTATTCCCAGAAGATGATGATATCCTAATAGAAGACTTGATGAAGTATGGGTTTGGAAAAGGATTGTTTCGAGATGTCAACAGCACAATAGAAGATGCCAGAGTCAAAGCAAATTCAGTGACTAAATACCTTAAAGCTTCTTGTTTGCTTTTGGACGGTAAAGAGGATGGATGTGTAAGGATGCATGATGTCATTCGAGATATGGCCATGTCAATTGCATTATCTGAAGATGGCTATGGGTTTTTGGTAAAAACTCGTTGTGAATTGAAAGAATGGCCAATGGATGCACATGAGGGATACTCGGCAATCTCATTAATGGAGAACAACATTTCCAAGCTGCCTAATGAGTTGGTATGTTCAAAACTCCAAATTTTATTGCTACAGAGAAAAATTGGTTTGAAAAATATCCCAGACACTTTTTTTCAGAGTCCGAAGGAATTAAAAGTCTTAGATCTTAGCCGCACCAATATTTTGTTAAGATCCCCATCATTCAATCTCATAACCAACCTCCAAGCTCTGTACTTAGATTATTGCGAGTCAATGATTGACATATCCATGCTTAAAAGCCTGAAAAAACTTGAGATTCTTAGTATAAGAGGATGTGATATTAAGGAATTTCCTAGAGAAATAGGACAACTGACCAATTTGAGGATGTTGGACCTCACTAATATTGGAGACATTGAGACAGTTCCATCTCAAGTGATTTCGAGGTTGCACAGATTAGAAGAACTGTACATGAAGTGTAAATTTGGGGATTGGGGCAGTAAAGTTGAGGGAGGCTTATATTCTTTCTTTTTTGGTAAAAATGCTGGCTTTGAAGAGTTAACCGACTTGTCGCATCTAAACATTTTGGAGGTGTCCATATTTGGTCCAGAGTGCTTGCCTAAAACCGTCGAGTTCGATCCAAATTGGGTAAGCTTCGATATATGTATCAACAAAAACTCGTTTAGGGGAATATCCTCTCTGCAACGTGACCATTCACATCATCATTCAAGAACTCTGAGCCTTGACACAACCATCAATACATTTCCGGATTGGTTTATCAACGTAGTGACAAAGAAAGCAGAGACGCTATTTTATTTCGAGTGCAAGGGCTTAAATAGCATTGTTGTAGAACATGACCATGGAAGACTACACAGACTGAAGCGGCTCGCTGTACATTCCAATGACAACTTGAAAGAGTTGTTGAACCTAACTACACGGGTCTCAAATGAACCTGTGTTTGAGAATTTGGAAGAATTGCATCTCAAGGACATGGGTTGCCTGAAGAAGTTATGTGTTGGTGAATTACCACTTGGGTCTCTACGGAAGCTGAAGGTATTGGACATCAGACAGTGTCGTGACTTGGTCGATGAACTTTTACCATCAAATTTACTGCGAATACTGCAAAATATGGAACAAATAATTTGTCATGATTTGAGGCAAATGAAATATGTGTTTGGATTTGAAGGGCTGAAGCCAGAACAGATTATCTTGACGAAATTGAGGAAGATGAGATTGTACAATCAACATAAGGTGTTACGCATATGGAAAGGTCCTGCTCCGCATGCAGTCTTCCATAATCTTCGAAGTTTGACGGTTGACTACTACACTAAACTGAAATACCTCTTCACATCCAATGTTGCTCAATGTCTTTTGCATTTGGAAGACCTGTGGCTACAAAGTTGCAGTAGCTTAGACAGAATTATTGAAGCAAGTGATGAGGAAATGATCGTCTTTCCAGAATTGAAGAACTTTATTTTGAAACATCTTCCACAGCTGAAAAGTTTTTGCAGTCCTATTTCAGTAGATATTGAATTCCCTTCATTGGAACACTTGTATGTGCGTGACTGCCCCCAGTTGATAATCTGTGCTTTTGACTTCAAAAGCAGGAACCATGTAAAACTGAATGATGAACAACATATGTCGTCTCTGTGCAGAAGGTTGTGGGATTTAGAATGA

Protein Analysis

1108

Amino Acids

127.65

Weight (kDa)

6.39

Isoelectric Point (pI)

47.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 23 - 195 1.5e-49 TIR domain
TIR_2 PF13676 26 - 137 5e-14 TIR domain
NB-ARC PF00931 211 - 375 8.9e-31 NB-ARC domain
LRR_14 PF23598 624 - 710 1.6e-09 Leucine-rich repeat region
LRR_RPS2 PF23247 872 - 1018 2.6e-24 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 965 - 1077 9.7e-17 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 2416
AciI CCGC 4 cut(s) 686, 1846, 2533, 2930
AclWI GGATC 7 cut(s) 191, 204, 910, 964, 977, 1860, 2282
AcoI YGGCCR 2 cut(s) 1590, 1661
AcsI RAATTY 9 cut(s) 336, 1148, 1498, 1755, 2000, 2132, 2614, 2755, 3182
AcuI CTGAAG 8 cut(s) 24, 908, 974, 1632, 2549, 2672, 2720, 2861
AfaI GTAC 7 cut(s) 375, 567, 741, 1907, 2120, 2544, 2888
AfiI CCNNNNNNNGG 2 cut(s) 2034, 3193
AjnI CCWGG 1 cut(s) 329
AjuI GAANNNNNNNTTGG 6 cut(s) 256, 288, 1355, 1387, 2664, 2696
AloI GAACNNNNNNTCC 2 cut(s) 1700, 1732
Alw21I GWGCWC 1 cut(s) 560
Alw44I GTGCAC 1 cut(s) 556
AlwI GGATC 7 cut(s) 191, 204, 910, 964, 977, 1860, 2282
Ama87I CYCGRG 1 cut(s) 7
Aor13HI TCCGGA 1 cut(s) 2416
AoxI GGCC 5 cut(s) 3, 797, 841, 1590, 1661
ApaLI GTGCAC 1 cut(s) 556
ApeKI GCWGC 4 cut(s) 12, 1259, 1383, 1723
ApoI RAATTY 9 cut(s) 336, 1148, 1498, 1755, 2000, 2132, 2614, 2755, 3182
ArsI GACNNNNNNTTYG 4 cut(s) 1223, 1255, 2779, 2811
AseI ATTAAT 2 cut(s) 159, 1700
Asp700I GAANNNNTTC 2 cut(s) 33, 102
AspLEI GCGC 1 cut(s) 824
AspS9I GGNCC 5 cut(s) 798, 841, 2044, 2255, 2921
AsuHPI GGTGA 4 cut(s) 107, 460, 1009, 2681
AsuII TTCGAA 1 cut(s) 2953
AsuNHI GCTAGC 1 cut(s) 1289
AvaI CYCGRG 1 cut(s) 7
AvaII GGWCC 3 cut(s) 2044, 2255, 2921
BaeGI GKGCMC 2 cut(s) 560, 1225
BalI TGGCCA 2 cut(s) 1592, 1663
BamHI GGATCC 2 cut(s) 196, 969
BarI GAAGNNNNNNTAC 2 cut(s) 145, 177
BbsI GAAGAC 7 cut(s) 105, 330, 719, 1425, 2521, 2932, 3039
Bbv12I GWGCWC 1 cut(s) 560
BbvI GCAGC 4 cut(s) 24, 1271, 1370, 1710
BccI CCATC 9 cut(s) 489, 774, 1298, 1547, 1610, 1879, 2086, 2411, 2758
BciT130I CCWGG 1 cut(s) 331
BciVI GTATCC 1 cut(s) 1676
BfaI CTAG 2 cut(s) 1290, 2007
BfmI CTRYAG 1 cut(s) 1766
BfuI GTATCC 1 cut(s) 1676
BglII AGATCT 2 cut(s) 832, 1837
BisI GCNGC 6 cut(s) 13, 1260, 1384, 1724, 1846, 2534
BlsI GCNGC 6 cut(s) 14, 1261, 1385, 1725, 1847, 2535
Bme1390I CCNGG 1 cut(s) 331
Bme18I GGWCC 3 cut(s) 2044, 2255, 2921
BmeT110I CYCGRG 1 cut(s) 7
BmgT120I GGNCC 5 cut(s) 798, 841, 2044, 2255, 2921
BmiI GGNNCC 4 cut(s) 198, 363, 971, 3260
BmrFI CCNGG 1 cut(s) 331
BmrI ACTGGG 2 cut(s) 969, 3217
BmsI GCATC 7 cut(s) 454, 1348, 1471, 1557, 1660, 2233, 2638
BmtI GCTAGC 1 cut(s) 1293
BmuI ACTGGG 2 cut(s) 969, 3217
BpiI GAAGAC 7 cut(s) 105, 330, 719, 1425, 2521, 2932, 3039
BplI GAGNNNNNCTC 2 cut(s) 1670, 1702
BpmI CTGGAG 1 cut(s) 621
Bpu14I TTCGAA 1 cut(s) 2953
BpuEI CTTGAG 5 cut(s) 44, 826, 1985, 2067, 2618
Bsa29I ATCGAT 1 cut(s) 228
BsaBI GATNNNNATC 1 cut(s) 1870
BsaI GGTCTC 3 cut(s) 1196, 2596, 2691
BsaJI CCNNGG 4 cut(s) 281, 636, 817, 2509
BsaWI WCCGGW 1 cut(s) 2416
Bsc4I CCNNNNNNNGG 2 cut(s) 2034, 3193
Bse1I ACTGG 4 cut(s) 604, 975, 1176, 3223
Bse3DI GCAATG 3 cut(s) 313, 540, 681
Bse8I GATNNNNATC 1 cut(s) 1870
BseAI TCCGGA 1 cut(s) 2416
BseBI CCWGG 1 cut(s) 331
BseCI ATCGAT 1 cut(s) 228
BseDI CCNNGG 4 cut(s) 281, 636, 817, 2509
BseJI GATNNNNATC 1 cut(s) 1870
BseLI CCNNNNNNNGG 2 cut(s) 2034, 3193
BseMI GCAATG 3 cut(s) 313, 540, 681
BseMII CTCAG 1 cut(s) 2378
BseNI ACTGG 4 cut(s) 604, 975, 1176, 3223
BseRI GAGGAG 1 cut(s) 872
BseSI GKGCMC 2 cut(s) 560, 1225
BseXI GCAGC 4 cut(s) 24, 1271, 1370, 1710
BseYI CCCAGC 1 cut(s) 838
BsgI GTGCAG 1 cut(s) 3322
BshFI GGCC 5 cut(s) 5, 799, 843, 1592, 1663
BshVI ATCGAT 1 cut(s) 228
BsiHKAI GWGCWC 1 cut(s) 560
BsiHKCI CYCGRG 1 cut(s) 7
BsiSI CCGG 1 cut(s) 2417
BslI CCNNNNNNNGG 2 cut(s) 2034, 3193
BsmBI CGTCTC 2 cut(s) 2447, 3300
BsmI GAATGC 1 cut(s) 1065
BsnI GGCC 5 cut(s) 5, 799, 843, 1592, 1663
Bso31I GGTCTC 3 cut(s) 1196, 2596, 2691
BsoBI CYCGRG 1 cut(s) 7
Bsp119I TTCGAA 1 cut(s) 2953
Bsp1286I GDGCHC 2 cut(s) 560, 1225
Bsp13I TCCGGA 1 cut(s) 2416
Bsp1407I TGTACA 4 cut(s) 739, 2118, 2542, 2886
Bsp143I GATC 8 cut(s) 196, 832, 915, 969, 1837, 1865, 2287, 3096
Bsp19I CCATGG 3 cut(s) 281, 636, 2509
BspACI CCGC 4 cut(s) 686, 1846, 2533, 2930
BspANI GGCC 5 cut(s) 5, 799, 843, 1592, 1663
BspCNI CTCAG 1 cut(s) 2379
BspDI ATCGAT 1 cut(s) 228
BspEI TCCGGA 1 cut(s) 2416
BspHI TCATGA 1 cut(s) 2797
BspLI GGNNCC 4 cut(s) 198, 363, 971, 3260
BspOI GCTAGC 1 cut(s) 1293
BspPI GGATC 7 cut(s) 191, 204, 910, 964, 977, 1860, 2282
BspQI GCTCTTC 2 cut(s) 174, 868
BspT104I TTCGAA 1 cut(s) 2953
BspTNI GGTCTC 3 cut(s) 1196, 2596, 2691
BsrDI GCAATG 3 cut(s) 313, 540, 681
BsrGI TGTACA 4 cut(s) 739, 2118, 2542, 2886
BsrI ACTGG 4 cut(s) 604, 975, 1176, 3223
BssECI CCNNGG 4 cut(s) 281, 636, 817, 2509
BssMI GATC 8 cut(s) 196, 832, 915, 969, 1837, 1865, 2287, 3096
BssT1I CCWWGG 4 cut(s) 281, 636, 817, 2509
Bst2UI CCWGG 1 cut(s) 331
Bst4CI ACNGT 8 cut(s) 55, 1352, 1544, 2074, 2118, 2278, 2721, 2965
Bst6I CTCTTC 4 cut(s) 174, 868, 2199, 2999
BstAUI TGTACA 4 cut(s) 739, 2118, 2542, 2886
BstBI TTCGAA 1 cut(s) 2953
BstC8I GCNNGC 6 cut(s) 633, 1291, 2197, 2267, 2538, 2934
BstDEI CTNAG 8 cut(s) 411, 561, 1834, 1841, 1909, 1973, 2387, 3061
BstDSI CCRYGG 3 cut(s) 281, 636, 2509
BstHHI GCGC 1 cut(s) 824
BstKTI GATC 8 cut(s) 199, 835, 918, 972, 1840, 1868, 2290, 3099
BstMBI GATC 8 cut(s) 196, 832, 915, 969, 1837, 1865, 2287, 3096
BstMWI GCNNNNNNNGC 5 cut(s) 620, 1208, 2487, 2770, 2933
BstNI CCWGG 1 cut(s) 331
BstNSI RCATGY 1 cut(s) 2936
BstSCI CCNGG 1 cut(s) 329
BstSFI CTRYAG 1 cut(s) 1766
BstSLI GKGCMC 2 cut(s) 560, 1225
BstV1I GCAGC 4 cut(s) 24, 1271, 1370, 1710
BstV2I GAAGAC 7 cut(s) 105, 330, 719, 1425, 2521, 2932, 3039
BstX2I RGATCY 6 cut(s) 196, 832, 915, 969, 1837, 1865
BstYI RGATCY 6 cut(s) 196, 832, 915, 969, 1837, 1865
Bsu15I ATCGAT 1 cut(s) 228
BsuI GTATCC 1 cut(s) 1676
BsuRI GGCC 5 cut(s) 5, 799, 843, 1592, 1663
BsuTUI ATCGAT 1 cut(s) 228
BtgI CCRYGG 3 cut(s) 281, 636, 2509
BtsI GCAGTG 2 cut(s) 461, 675
BtsIMutI CAGTG 7 cut(s) 461, 597, 675, 1183, 1202, 1509, 2726
Cac8I GCNNGC 6 cut(s) 633, 1291, 2197, 2267, 2538, 2934
CciI TCATGA 1 cut(s) 2797
CfoI GCGC 1 cut(s) 824
Cfr13I GGNCC 5 cut(s) 798, 841, 2044, 2255, 2921
ClaI ATCGAT 1 cut(s) 228
CseI GACGC 1 cut(s) 2464
Csp6I GTAC 7 cut(s) 374, 566, 740, 1906, 2119, 2543, 2887
CviQI GTAC 7 cut(s) 374, 566, 740, 1906, 2119, 2543, 2887
DdeI CTNAG 8 cut(s) 411, 561, 1834, 1841, 1909, 1973, 2387, 3061
DpnI GATC 8 cut(s) 198, 834, 917, 971, 1839, 1867, 2289, 3098
DpnII GATC 8 cut(s) 196, 832, 915, 969, 1837, 1865, 2287, 3096
DraI TTTAAA 2 cut(s) 471, 1318
EaeI YGGCCR 2 cut(s) 1590, 1661
Eam1104I CTCTTC 4 cut(s) 174, 868, 2199, 2999
EarI CTCTTC 4 cut(s) 174, 868, 2199, 2999
Eco130I CCWWGG 4 cut(s) 281, 636, 817, 2509
Eco31I GGTCTC 3 cut(s) 1196, 2596, 2691
Eco32I GATATC 2 cut(s) 512, 1408
Eco47I GGWCC 3 cut(s) 2044, 2255, 2921
Eco57I CTGAAG 8 cut(s) 24, 908, 974, 1632, 2549, 2672, 2720, 2861
Eco88I CYCGRG 1 cut(s) 7
EcoO109I RGGNCCY 1 cut(s) 2921
EcoRI GAATTC 3 cut(s) 336, 1148, 3182
EcoRII CCWGG 1 cut(s) 329
EcoRV GATATC 2 cut(s) 512, 1408
EcoT14I CCWWGG 4 cut(s) 281, 636, 817, 2509
EcoT22I ATGCAT 1 cut(s) 1572
ErhI CCWWGG 4 cut(s) 281, 636, 817, 2509
Esp3I CGTCTC 2 cut(s) 2447, 3300
FalI AAGNNNNNCTT 2 cut(s) 1355, 1387
FauNDI CATATG 2 cut(s) 2912, 3288
Fnu4HI GCNGC 6 cut(s) 13, 1260, 1384, 1724, 1846, 2534
Fsp4HI GCNGC 6 cut(s) 13, 1260, 1384, 1724, 1846, 2534
FspBI CTAG 2 cut(s) 1290, 2007
GlaI GCGC 1 cut(s) 823
GluI GCNGC 6 cut(s) 13, 1260, 1384, 1724, 1846, 2534
GsaI CCCAGC 1 cut(s) 842
GsuI CTGGAG 1 cut(s) 621
HaeIII GGCC 5 cut(s) 5, 799, 843, 1592, 1663
HapII CCGG 1 cut(s) 2417
HgaI GACGC 1 cut(s) 2464
HhaI GCGC 1 cut(s) 824
Hin6I GCGC 1 cut(s) 822
HinP1I GCGC 1 cut(s) 822
HincII GTYRAC 4 cut(s) 51, 1465, 2211, 2968
HindII GTYRAC 4 cut(s) 51, 1465, 2211, 2968
HindIII AAGCTT 3 cut(s) 986, 1521, 2302
HinfI GANTC 7 cut(s) 29, 233, 1094, 1488, 1813, 1922, 1969
HpaI GTTAAC 1 cut(s) 2211
HpaII CCGG 1 cut(s) 2417
HphI GGTGA 4 cut(s) 107, 460, 1009, 2681
Hpy166II GTNNAC 7 cut(s) 51, 558, 597, 740, 1465, 2211, 2968
Hpy8I GTNNAC 7 cut(s) 51, 558, 597, 740, 1465, 2211, 2968
Hpy99I CGWCG 1 cut(s) 2282
HpyCH4III ACNGT 8 cut(s) 55, 1352, 1544, 2074, 2118, 2278, 2721, 2965
HpyCH4IV ACGT 3 cut(s) 1285, 2356, 2433
HpyF10VI GCNNNNNNNGC 5 cut(s) 620, 1208, 2487, 2770, 2933
HpyF3I CTNAG 8 cut(s) 411, 561, 1834, 1841, 1909, 1973, 2387, 3061
HpySE526I ACGT 3 cut(s) 1285, 2356, 2433
HspAI GCGC 1 cut(s) 822
Kpn2I TCCGGA 1 cut(s) 2416
KspAI GTTAAC 1 cut(s) 2211
Kzo9I GATC 8 cut(s) 196, 832, 915, 969, 1837, 1865, 2287, 3096
LguI GCTCTTC 2 cut(s) 174, 868
LmnI GCTCC 1 cut(s) 2932
Lsp1109I GCAGC 4 cut(s) 24, 1271, 1370, 1710
LweI GCATC 7 cut(s) 454, 1348, 1471, 1557, 1660, 2233, 2638
MaeI CTAG 2 cut(s) 1290, 2007
MaeII ACGT 3 cut(s) 1285, 2356, 2433
MaeIII GTNAC 8 cut(s) 673, 977, 1504, 2357, 2437, 2726, 2904, 3212
MalI GATC 8 cut(s) 198, 834, 917, 971, 1839, 1867, 2289, 3098
MboI GATC 8 cut(s) 196, 832, 915, 969, 1837, 1865, 2287, 3096
MfeI CAATTG 2 cut(s) 573, 1599
MflI RGATCY 6 cut(s) 196, 832, 915, 969, 1837, 1865
MhlI GDGCHC 2 cut(s) 560, 1225
MlsI TGGCCA 2 cut(s) 1592, 1663
MluNI TGGCCA 2 cut(s) 1592, 1663
MlyI GAGTC 3 cut(s) 1497, 1822, 1931
MmeI TCCRAC 1 cut(s) 2022
Mox20I TGGCCA 2 cut(s) 1592, 1663
Mph1103I ATGCAT 1 cut(s) 1572
MroI TCCGGA 1 cut(s) 2416
MroXI GAANNNNTTC 2 cut(s) 33, 102
MscI TGGCCA 2 cut(s) 1592, 1663
MslI CAYNNNNRTG 5 cut(s) 686, 1940, 2508, 2550, 3204
Msp20I TGGCCA 2 cut(s) 1592, 1663
MspA1I CMGCKG 2 cut(s) 368, 3145
MspI CCGG 1 cut(s) 2417
MspR9I CCNGG 1 cut(s) 331
MunI CAATTG 2 cut(s) 573, 1599
Mva1269I GAATGC 1 cut(s) 1065
MvaI CCWGG 1 cut(s) 331
MwoI GCNNNNNNNGC 5 cut(s) 620, 1208, 2487, 2770, 2933
NcoI CCATGG 3 cut(s) 281, 636, 2509
NdeI CATATG 2 cut(s) 2912, 3288
NdeII GATC 8 cut(s) 196, 832, 915, 969, 1837, 1865, 2287, 3096
NheI GCTAGC 1 cut(s) 1289
NlaIV GGNNCC 4 cut(s) 198, 363, 971, 3260
NmeAIII GCCGAG 1 cut(s) 1667
NmuCI GTSAC 6 cut(s) 673, 1504, 2357, 2437, 2726, 3212
NsiI ATGCAT 1 cut(s) 1572
NspI RCATGY 1 cut(s) 2936
NspV TTCGAA 1 cut(s) 2953
PaeI GCATGC 1 cut(s) 2936
PaeR7I CTCGAG 1 cut(s) 7
PagI TCATGA 1 cut(s) 2797
PciSI GCTCTTC 2 cut(s) 174, 868
PctI GAATGC 1 cut(s) 1065
PdmI GAANNNNTTC 2 cut(s) 33, 102
PfeI GAWTC 4 cut(s) 29, 233, 1094, 1969
PflFI GACNNNGTC 2 cut(s) 2721, 2732
PkrI GCNGC 6 cut(s) 14, 1261, 1385, 1725, 1847, 2535
PleI GAGTC 3 cut(s) 1496, 1821, 1930
PpsI GAGTC 3 cut(s) 1496, 1821, 1930
PpuMI RGGWCCY 1 cut(s) 2921
PshBI ATTAAT 2 cut(s) 159, 1700
Psp5II RGGWCCY 1 cut(s) 2921
Psp6I CCWGG 1 cut(s) 329
PspFI CCCAGC 1 cut(s) 838
PspGI CCWGG 1 cut(s) 329
PspN4I GGNNCC 4 cut(s) 198, 363, 971, 3260
PspPI GGNCC 5 cut(s) 798, 841, 2044, 2255, 2921
PspPPI RGGWCCY 1 cut(s) 2921
PspXI VCTCGAGB 1 cut(s) 7
PsuI RGATCY 6 cut(s) 196, 832, 915, 969, 1837, 1865
PsyI GACNNNGTC 2 cut(s) 2721, 2732
PvuII CAGCTG 2 cut(s) 368, 3145
RsaI GTAC 7 cut(s) 375, 567, 741, 1907, 2120, 2544, 2888
RsaNI GTAC 7 cut(s) 374, 566, 740, 1906, 2119, 2543, 2887
RseI CAYNNNNRTG 5 cut(s) 686, 1940, 2508, 2550, 3204
SapI GCTCTTC 2 cut(s) 174, 868
SatI GCNGC 6 cut(s) 13, 1260, 1384, 1724, 1846, 2534
Sau3AI GATC 8 cut(s) 196, 832, 915, 969, 1837, 1865, 2287, 3096
Sau96I GGNCC 5 cut(s) 798, 841, 2044, 2255, 2921
SchI GAGTC 3 cut(s) 1497, 1822, 1931
ScrFI CCNGG 1 cut(s) 331
SduI GDGCHC 2 cut(s) 560, 1225
SfaNI GCATC 7 cut(s) 454, 1348, 1471, 1557, 1660, 2233, 2638
SfcI CTRYAG 1 cut(s) 1766
Sfr274I CTCGAG 1 cut(s) 7
SfuI TTCGAA 1 cut(s) 2953
SinI GGWCC 3 cut(s) 2044, 2255, 2921
SlaI CTCGAG 1 cut(s) 7
SmiMI CAYNNNNRTG 5 cut(s) 686, 1940, 2508, 2550, 3204
SmlI CTYRAG 6 cut(s) 7, 59, 805, 1964, 2082, 2633
SmoI CTYRAG 6 cut(s) 7, 59, 805, 1964, 2082, 2633
SphI GCATGC 1 cut(s) 2936
SsiI CCGC 4 cut(s) 686, 1846, 2533, 2930
SspI AATATT 2 cut(s) 1855, 2056
SspMI CTAG 2 cut(s) 1290, 2007
StyD4I CCNGG 1 cut(s) 329
StyI CCWWGG 4 cut(s) 281, 636, 817, 2509
TaaI ACNGT 8 cut(s) 55, 1352, 1544, 2074, 2118, 2278, 2721, 2965
TaiI ACGT 3 cut(s) 1288, 2359, 2436
TatI WGTACW 5 cut(s) 739, 1905, 2118, 2542, 2886
TauI GCSGC 2 cut(s) 1848, 2536
TfiI GAWTC 4 cut(s) 29, 233, 1094, 1969
TscAI CASTG 7 cut(s) 461, 604, 682, 1183, 1209, 1509, 2726
TseFI GTSAC 6 cut(s) 673, 1504, 2357, 2437, 2726, 3212
TseI GCWGC 4 cut(s) 12, 1259, 1383, 1723
Tsp45I GTSAC 6 cut(s) 673, 1504, 2357, 2437, 2726, 3212
TspGWI ACGGA 1 cut(s) 2707
TspRI CASTG 7 cut(s) 461, 604, 682, 1183, 1209, 1509, 2726
Tth111I GACNNNGTC 2 cut(s) 2721, 2732
VneI GTGCAC 1 cut(s) 556
VpaK11BI GGWCC 3 cut(s) 2044, 2255, 2921
VspI ATTAAT 2 cut(s) 159, 1700
XapI RAATTY 9 cut(s) 336, 1148, 1498, 1755, 2000, 2132, 2614, 2755, 3182
XceI RCATGY 1 cut(s) 2936
XhoI CTCGAG 1 cut(s) 7
XmnI GAANNNNTTC 2 cut(s) 33, 102
XspI CTAG 2 cut(s) 1290, 2007
Zsp2I ATGCAT 1 cut(s) 1572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.