RchiOBHm_Chr5g0079811

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000085
Physical Location & Seq
Reverse (-)
85568835 .. 85574051
5217 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35418

Sequence Viewer

Length: 3489 bp
ATGGAATTCTATTACTTCCAGATGGCCATGGCGATTATTGTGGTAATTGTGATATGGATCGGTGTCATAGCTACTATTAAGTGGATATGGACTCTAGTGAAGCATCGGTTGGGTTACATGGTTGAAAGCATGAGCCAACATCGCAGGATTTTCGTCAACATCGATGCCTCCAGACCTCTTCCTTCACCATCAGCTGAATCATCAGCAGCTCGTCGATGGAATTATGATGTGTTCTTGAGTTTCAGGGGTCCTGACACTCGCAAGGGTATTACATTTGAATTATATGATCGACTGCACAGGAGGGGAATTAAAACATTCATGGATGACCAAGATCTTCAAGTAGGGGATACTATTTCTCCCACTCTACTAAAAGCAATTGAAGAATCAAGGTTGGCAATTGTTGTTCTGTCCGAAAAGTATGCTTCTTCTACTTGGTGTTTGGAGGAACTTACAAAGATCTGTGAGTGCATGAAAGACGACAACAGAATTCTTCCACTTTTTTATAATGTCGAGCCTACTGATGTAAGATATCAGAAGAGAAGTTTCGAAGAAGCTTTCGCCAAATATGAAAATTCTGGGCGACATAGATCGGAGAAGGTCCAACAATGGAGAGATGCTTTAAACAAAGTGGCTCATTTCTCTGGTTGGGATTCAAAGAAATACAAGACTGAAAGAGAACTCATCGAAGACATTGTGGAATATGTCTGTAGTAAAGTACAACCTATTGCAATCAACATGGGAGATTTTGAAGAATTTGAAGCAACAAGACAAGCCATGAATATGGTTATGAAAGGGCTAGAAGACGACGAGGTCACTGCCATTGGAGTCTGCGGAATGGGAGGCGTCGGCAAGACAACCATGGTAAAACATATCAGTGATCAAGCTCGAAAAAATGGAATCTTTCGTCATGTAATTATGGCTACTATATCACAAAGCCCTGACTTGAGGAAAATTCAAGGCCAATTGGCAGATCTGTTGGGCTTCGAATTCATGGAGGAGACTGAAATTGGAAGAGCAGCTAGATTGAGTAAGGAGATAATGAGAAGAAATAAGATCCTCATAATCCTTGATGACATTTGGGAGAGGATGGATATATCAAGAATAGGAATACCTAGCTACGAGGAACTTCAAAAGTGCAATTCCAAAGTCCTACTCACCACAAGGATATGGAATGTCTGTCATGTCATGAGATGCCAAGAAAAGATCACCCTCAATATCCTATCAAAAGAGGATTCTTGGACATTGTTTGTGAGAAATGCAGGAAGGTCTTTTGAATCCACCAATTTTGAGAATGTAGCGAGGAATGTAGCTGGAGAATGTAGGGGTCTACCGATTGCATTGATAGCAGTTGCAAGGGCACTCGGCGATAAAGAATTGGTGGAATGGGAAAGAGCAGCTCAACGACTAGAGAAGTCACAAACTGCCAACCCTGACGATAAAGGAGATGCATCTGAATGTATAAGATTAAGTTATGATTACTTGAAAGATGAGGATTACAAGTCATGCTTCTTGCTCTGTTGCTTATTTCCAGAAGACTATGACATCCAAATAGAAAACTTGTTCAACTATGTGATAGGGAAAGGATTGTTTAGAGATGCTGAGACCATTGAAGAAGCCAGAGGAACAACAAATTCGGTGGTTGCGCACCTGAAAAATTGTAGCTTGCTTTTGGATAGTGATGAAGATGGATGTGTAAGAATGCATGATGTCATCCGGGATACAGCCATGATAATTGCAAAATCTAAAGACGGGCATGGGTTTTTGGTAAAAGCTGGGTCTGGTTTAAAGCATTGGTCATGTCGATTACATGAAGACTACTCTGCAATTTCACTAATGAAGAACAAAATTCGCAAGCTACCTGAAGAGTTGGTATGTCCAAAACTCCAGATTTTATTACTACAAGAGAATGCTGATTTAACTGAGATTCCAGAAAAATTTCTCCAAAGTGCAAATGAACTAAGGGTCTTAGATCTTGGCCTCACTAGTGTTTTATCAGTACCCCAATCATTGAGTCTCCTATCAAACCTCCAAGCTTTGTATTTAGATTTTTGCAAGAAAAGAGTTGACATTTCCATAATTGGAAAACTTACTAAGCTTGAGATTCTTAGTATGAGAGGTTATCCTGTTAAGGAGTTGTCGAGAGAATTGTGGAGAGAAGTAGGAAAATTGACTAATCTAAGGATGTTGGATGTCGGTGGTGTTAGTTTGGGGGGTGGAGGTATTGTCACAATTCCCTCTAAAGTGATATCAAAGTTGCATAGATTAGAAGAACTGTACATGCTGTACTGTGGATTTGAGGACTGGGGAAGTCGAGTTGAGGGAGAAGGAGAAGAAACATATATTAGCTTTGATGAGTTAACTGGTTTGTCAAATTTGAAAATTGTGCAGGTTTGCATATCTGATGCAAAATGCATCCCAAAAGAAGTTGAGGTCGAACCAAATTGGGTTTACTTTGATATACGTATCGATGGCTGGTCAAGCAATCCATACAAGCGAAACGTTCCTAACTCAAGATCCTCGTCTCTTAACACAACCATCAGTAGCTTACCAGACTGGTTTGTCAATGTGGTGACAAAGAAATCAGAGAAGCTACTGTTTGAAGAAGACTGCAAAGGGTTAAGTAACATTCTTATGGAATATGACCATGGGAGGTTACATGGACTGAAGCATCTCTCTGTAATTGGTCCCAATAAGAGCTTGAAAGAGTTGATGAACACAACAACACGGATTAAACAAGAACCTGTATTTGAGAACTTGGAAGAGTTGCATCTTAGTCGTCTGGACTGCATTAAGGAGTTGTGTGTTGGTGACTTACCACCTGGTTCTCTATTCAATGTGAAATTATTGAGGATCCGTGATTGTCGTAACTTGGGGAATGTACTGTTGCCTTCAAAATTGTTGCAGAGACTACCAAATCTGGAAATAGTATATCTTTATAGAATATGTAAATTGGAATATGTGTTTGGATGCGAAGGGTTTGAGCCAGAACATTCAAAATTGAGAGAAGTGGCATTGTTGGAGCTAGATGCAATAAGAAGCATATGTAATGGTCCGGTTCCCCGTGCTATGTTCCGGACTCTTAAAAGTTTGTTCATTTACCGTTGTAAGTCTTTGGGAAGCCTCTTCACATCTGATGTAGCGCATTGTCTTTTACAATTGGAAGAGCTTTGGGTAGAGAATTGCCCTTTGTTGGAAAGAGTAATTGAAACAAGCAAGGAAACAGTTAACGACAACAATAAGATTGTTCTTTCAAAATTAAAGAAGTTAGTCTTGAAGGAACTTCCTGTGTTGAACAGTGGAAGTGCTACTACTAATATTGATATCGAGTGTCTTTCATTGGAACATTTGTATGTGAAGGACTGCCCCCATTTCTCATTTCCAACCTCTGCTTCGGACTATTTCCACAGCAGGAACGAAGTCCAACTCGATGATCAACAACAGCACGACGTTCTATATCGCAGAATTTGGAGTCCAACATTGGAAAGCTGA

Protein Analysis

1162

Amino Acids

132.99

Weight (kDa)

6.0

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 74 - 243 4.9e-49 TIR domain
TIR_2 PF13676 77 - 172 1.1e-15 TIR domain
NB-ARC PF00931 257 - 420 2.2e-28 NB-ARC domain
LRR_14 PF23598 632 - 765 8e-11 Leucine-rich repeat region
LRR_RPS2 PF23247 918 - 1061 3.1e-20 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1003 - 1125 4e-15 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 504
AasI GACNNNNNNGTC 2 cut(s) 809, 2558
Acc16I TGCGCA 1 cut(s) 1644
Acc36I ACCTGC 1 cut(s) 2377
AccI GTMKAC 1 cut(s) 1327
AccIII TCCGGA 1 cut(s) 3072
AciI CCGC 1 cut(s) 831
AclI AACGTT 1 cut(s) 2499
AclWI GGATC 5 cut(s) 65, 1048, 2508, 2845, 2858
AcoI YGGCCR 1 cut(s) 24
AcuI CTGAAG 2 cut(s) 1881, 2684
AcyI GRCGYC 1 cut(s) 843
AfaI GTAC 5 cut(s) 717, 1998, 2276, 2285, 2880
AfiI CCNNNNNNNGG 1 cut(s) 3190
AhlI ACTAGT 1 cut(s) 1982
AjnI CCWGG 1 cut(s) 2818
AjuI GAANNNNNNNTTGG 6 cut(s) 92, 124, 2946, 2978, 3373, 3405
Alw26I GTCTC 5 cut(s) 992, 1595, 2018, 2526, 2899
AlwI GGATC 5 cut(s) 65, 1048, 2508, 2845, 2858
Aor13HI TCCGGA 1 cut(s) 3072
AoxI GGCC 3 cut(s) 24, 958, 1975
ApeKI GCWGC 3 cut(s) 206, 1016, 1394
Asp700I GAANNNNTTC 1 cut(s) 1935
AspLEI GCGC 2 cut(s) 1645, 3142
AspS9I GGNCC 4 cut(s) 248, 598, 2684, 3050
AsuC2I CCSGG 1 cut(s) 1715
AsuHPI GGTGA 5 cut(s) 177, 1147, 1198, 2581, 2819
AsuII TTCGAA 2 cut(s) 546, 984
AvaII GGWCC 4 cut(s) 248, 598, 2684, 3050
BaeGI GKGCMC 1 cut(s) 1360
BalI TGGCCA 1 cut(s) 26
BamHI GGATCC 1 cut(s) 2850
BarI GAAGNNNNNNTAC 2 cut(s) 3292, 3324
BbsI GAAGAC 5 cut(s) 693, 807, 1539, 1818, 2610
BbvI GCAGC 3 cut(s) 218, 1028, 1406
BccI CCATC 7 cut(s) 16, 196, 210, 1081, 1679, 2462, 2543
BcgI CGANNNNNNTGC 8 cut(s) 133, 167, 401, 435, 797, 831, 2756, 2790
BciT130I CCWGG 1 cut(s) 2820
BciVI GTATCC 2 cut(s) 340, 1711
BclI TGATCA 2 cut(s) 877, 3430
BcnI CCSGG 1 cut(s) 1715
BcoDI GTCTC 5 cut(s) 992, 1595, 2018, 2526, 2899
BcuI ACTAGT 1 cut(s) 1982
BfaI CTAG 7 cut(s) 95, 797, 1020, 1113, 1406, 1983, 3023
BfmI CTRYAG 1 cut(s) 706
BfuAI ACCTGC 1 cut(s) 2377
BfuI GTATCC 2 cut(s) 340, 1711
BglII AGATCT 4 cut(s) 331, 456, 970, 1969
BisI GCNGC 3 cut(s) 207, 1017, 1395
BlsI GCNGC 3 cut(s) 208, 1018, 1396
Bme1390I CCNGG 2 cut(s) 1715, 2820
Bme18I GGWCC 4 cut(s) 248, 598, 2684, 3050
BmgT120I GGNCC 4 cut(s) 248, 598, 2684, 3050
BmiI GGNNCC 4 cut(s) 249, 2686, 2852, 3057
BmrFI CCNGG 2 cut(s) 1715, 2820
BmrI ACTGGG 1 cut(s) 2311
BmuI ACTGGG 1 cut(s) 2311
BpiI GAAGAC 5 cut(s) 693, 807, 1539, 1818, 2610
BpmI CTGGAG 3 cut(s) 154, 1332, 1868
Bpu14I TTCGAA 2 cut(s) 546, 984
BpuEI CTTGAG 4 cut(s) 256, 964, 2117, 2494
BpuMI CCSGG 1 cut(s) 1715
Bsa29I ATCGAT 2 cut(s) 162, 2466
BsaAI YACGTR 1 cut(s) 2462
BsaBI GATNNNNATC 1 cut(s) 56
BsaHI GRCGYC 1 cut(s) 843
BsaI GGTCTC 1 cut(s) 1595
BsaJI CCNNGG 3 cut(s) 27, 858, 2644
BsaWI WCCGGW 2 cut(s) 3052, 3072
BsaXI ACNNNNNCTCC 4 cut(s) 340, 370, 2208, 2238
Bsc4I CCNNNNNNNGG 1 cut(s) 3190
Bse1I ACTGG 3 cut(s) 2306, 2365, 2558
Bse8I GATNNNNATC 1 cut(s) 56
BseAI TCCGGA 1 cut(s) 3072
BseBI CCWGG 1 cut(s) 2820
BseCI ATCGAT 2 cut(s) 162, 2466
BseDI CCNNGG 3 cut(s) 27, 858, 2644
BseGI GGATG 9 cut(s) 328, 1092, 1542, 1694, 1710, 2187, 2194, 2412, 2972
BseJI GATNNNNATC 1 cut(s) 56
BseLI CCNNNNNNNGG 1 cut(s) 3190
BseMII CTCAG 2 cut(s) 1590, 1911
BseNI ACTGG 3 cut(s) 2306, 2365, 2558
BseRI GAGGAG 1 cut(s) 1010
BseSI GKGCMC 1 cut(s) 1360
BseXI GCAGC 3 cut(s) 218, 1028, 1406
BseYI CCCAGC 1 cut(s) 1772
BsgI GTGCAG 2 cut(s) 278, 2405
BshFI GGCC 3 cut(s) 26, 960, 1977
BshVI ATCGAT 2 cut(s) 162, 2466
BsiSI CCGG 3 cut(s) 1714, 3053, 3073
BslFI GGGAC 1 cut(s) 2670
BslI CCNNNNNNNGG 1 cut(s) 3190
BsmAI GTCTC 5 cut(s) 992, 1595, 2018, 2526, 2899
BsmBI CGTCTC 1 cut(s) 2526
BsmFI GGGAC 1 cut(s) 2670
BsmI GAATGC 2 cut(s) 1704, 1912
BsnI GGCC 3 cut(s) 26, 960, 1977
Bso31I GGTCTC 1 cut(s) 1595
Bsp119I TTCGAA 2 cut(s) 546, 984
Bsp1286I GDGCHC 1 cut(s) 1360
Bsp13I TCCGGA 1 cut(s) 3072
Bsp1407I TGTACA 1 cut(s) 2274
Bsp19I CCATGG 3 cut(s) 27, 858, 2644
BspACI CCGC 1 cut(s) 831
BspANI GGCC 3 cut(s) 26, 960, 1977
BspCNI CTCAG 2 cut(s) 1591, 1912
BspDI ATCGAT 2 cut(s) 162, 2466
BspEI TCCGGA 1 cut(s) 3072
BspHI TCATGA 1 cut(s) 1185
BspLI GGNNCC 4 cut(s) 249, 2686, 2852, 3057
BspMI ACCTGC 1 cut(s) 2377
BspPI GGATC 5 cut(s) 65, 1048, 2508, 2845, 2858
BspQI GCTCTTC 2 cut(s) 1006, 3156
BspT104I TTCGAA 2 cut(s) 546, 984
BspTNI GGTCTC 1 cut(s) 1595
BsrGI TGTACA 1 cut(s) 2274
BsrI ACTGG 3 cut(s) 2306, 2365, 2558
BssECI CCNNGG 3 cut(s) 27, 858, 2644
BssNI GRCGYC 1 cut(s) 843
BssT1I CCWWGG 3 cut(s) 27, 858, 2644
Bst2UI CCWGG 1 cut(s) 2820
Bst4CI ACNGT 7 cut(s) 2274, 2288, 2595, 2883, 3101, 3223, 3296
Bst6I CTCTTC 7 cut(s) 183, 530, 1006, 1857, 2754, 3128, 3156
BstACI GRCGYC 1 cut(s) 843
BstAUI TGTACA 1 cut(s) 2274
BstBAI YACGTR 1 cut(s) 2462
BstBI TTCGAA 2 cut(s) 546, 984
BstC8I GCNNGC 2 cut(s) 1664, 1853
BstDEI CTNAG 8 cut(s) 1599, 1920, 1958, 1966, 2091, 2105, 2177, 2771
BstDSI CCRYGG 3 cut(s) 27, 858, 2644
BstF5I GGATG 9 cut(s) 328, 1092, 1542, 1694, 1710, 2187, 2194, 2412, 2972
BstHHI GCGC 2 cut(s) 1645, 3142
BstMAI GTCTC 5 cut(s) 992, 1595, 2018, 2526, 2899
BstMWI GCNNNNNNNGC 3 cut(s) 141, 1343, 2478
BstNI CCWGG 1 cut(s) 2820
BstNSI RCATGY 1 cut(s) 2281
BstSCI CCNGG 2 cut(s) 1713, 2818
BstSFI CTRYAG 1 cut(s) 706
BstSLI GKGCMC 1 cut(s) 1360
BstSNI TACGTA 1 cut(s) 2462
BstV1I GCAGC 3 cut(s) 218, 1028, 1406
BstV2I GAAGAC 5 cut(s) 693, 807, 1539, 1818, 2610
BstX2I RGATCY 7 cut(s) 331, 456, 970, 1053, 1969, 2513, 2850
BstXI CCANNNNNNTGG 1 cut(s) 781
BstYI RGATCY 7 cut(s) 331, 456, 970, 1053, 1969, 2513, 2850
Bsu15I ATCGAT 2 cut(s) 162, 2466
BsuI GTATCC 2 cut(s) 340, 1711
BsuRI GGCC 3 cut(s) 26, 960, 1977
BsuTUI ATCGAT 2 cut(s) 162, 2466
BtgI CCRYGG 3 cut(s) 27, 858, 2644
BtgZI GCGATG 1 cut(s) 125
BtsCI GGATG 9 cut(s) 328, 1092, 1542, 1694, 1710, 2187, 2194, 2412, 2972
BtsI GCAGTG 1 cut(s) 813
BtsIMutI CAGTG 3 cut(s) 813, 880, 3301
BveI ACCTGC 1 cut(s) 2377
Cac8I GCNNGC 2 cut(s) 1664, 1853
CciI TCATGA 1 cut(s) 1185
CfoI GCGC 2 cut(s) 1645, 3142
Cfr13I GGNCC 4 cut(s) 248, 598, 2684, 3050
ClaI ATCGAT 2 cut(s) 162, 2466
CseI GACGC 1 cut(s) 832
CsiI ACCWGGT 1 cut(s) 2818
Csp6I GTAC 5 cut(s) 716, 1997, 2275, 2284, 2879
CspCI CAANNNNNGTGG 4 cut(s) 1267, 1302, 1617, 1652
CviQI GTAC 5 cut(s) 716, 1997, 2275, 2284, 2879
DdeI CTNAG 8 cut(s) 1599, 1920, 1958, 1966, 2091, 2105, 2177, 2771
DraI TTTAAA 2 cut(s) 621, 1785
DrdI GACNNNNNNGTC 2 cut(s) 809, 2558
DseDI GACNNNNNNGTC 2 cut(s) 809, 2558
EaeI YGGCCR 1 cut(s) 24
Eam1104I CTCTTC 7 cut(s) 183, 530, 1006, 1857, 2754, 3128, 3156
EarI CTCTTC 7 cut(s) 183, 530, 1006, 1857, 2754, 3128, 3156
Eco105I TACGTA 1 cut(s) 2462
Eco130I CCWWGG 3 cut(s) 27, 858, 2644
Eco31I GGTCTC 1 cut(s) 1595
Eco32I GATATC 3 cut(s) 530, 2247, 3322
Eco47I GGWCC 4 cut(s) 248, 598, 2684, 3050
Eco57I CTGAAG 2 cut(s) 1881, 2684
EcoO109I RGGNCCY 1 cut(s) 248
EcoRI GAATTC 3 cut(s) 5, 486, 986
EcoRII CCWGG 1 cut(s) 2818
EcoRV GATATC 3 cut(s) 530, 2247, 3322
EcoT14I CCWWGG 3 cut(s) 27, 858, 2644
EcoT22I ATGCAT 3 cut(s) 1450, 1704, 2414
ErhI CCWWGG 3 cut(s) 27, 858, 2644
Esp3I CGTCTC 1 cut(s) 2526
FalI AAGNNNNNCTT 4 cut(s) 1490, 1522, 3254, 3286
FaqI GGGAC 1 cut(s) 2670
FauNDI CATATG 1 cut(s) 3041
FbaI TGATCA 2 cut(s) 877, 3430
FblI GTMKAC 1 cut(s) 1327
Fnu4HI GCNGC 3 cut(s) 207, 1017, 1395
FokI GGATG 9 cut(s) 335, 1099, 1529, 1697, 1701, 2194, 2201, 2399, 2979
Fsp4HI GCNGC 3 cut(s) 207, 1017, 1395
FspBI CTAG 7 cut(s) 95, 797, 1020, 1113, 1406, 1983, 3023
FspI TGCGCA 1 cut(s) 1644
GlaI GCGC 2 cut(s) 1644, 3141
GluI GCNGC 3 cut(s) 207, 1017, 1395
GsaI CCCAGC 1 cut(s) 1776
GsuI CTGGAG 3 cut(s) 154, 1332, 1868
HaeIII GGCC 3 cut(s) 26, 960, 1977
HapII CCGG 3 cut(s) 1714, 3053, 3073
HgaI GACGC 1 cut(s) 832
HhaI GCGC 2 cut(s) 1645, 3142
Hin1I GRCGYC 1 cut(s) 843
Hin6I GCGC 2 cut(s) 1643, 3140
HinP1I GCGC 2 cut(s) 1643, 3140
HincII GTYRAC 4 cut(s) 157, 2065, 2358, 3226
HindII GTYRAC 4 cut(s) 157, 2065, 2358, 3226
HindIII AAGCTT 3 cut(s) 552, 2031, 2093
HpaI GTTAAC 2 cut(s) 2358, 3226
HpaII CCGG 3 cut(s) 1714, 3053, 3073
HphI GGTGA 5 cut(s) 177, 1147, 1198, 2581, 2819
Hpy166II GTNNAC 6 cut(s) 157, 1328, 2065, 2358, 2449, 3226
Hpy188I TCNGA 8 cut(s) 412, 534, 592, 1453, 2401, 2584, 3133, 3394
Hpy8I GTNNAC 6 cut(s) 157, 1328, 2065, 2358, 2449, 3226
Hpy99I CGWCG 4 cut(s) 216, 809, 848, 3449
HpyAV CCTTC 8 cut(s) 192, 589, 1257, 2318, 2898, 2966, 3268, 3349
HpyCH4III ACNGT 7 cut(s) 2274, 2288, 2595, 2883, 3101, 3223, 3296
HpyCH4IV ACGT 3 cut(s) 2461, 2499, 3447
HpyF10VI GCNNNNNNNGC 3 cut(s) 141, 1343, 2478
HpyF3I CTNAG 8 cut(s) 1599, 1920, 1958, 1966, 2091, 2105, 2177, 2771
HpySE526I ACGT 3 cut(s) 2461, 2499, 3447
Hsp92I GRCGYC 1 cut(s) 843
HspAI GCGC 2 cut(s) 1643, 3140
Kpn2I TCCGGA 1 cut(s) 3072
Ksp22I TGATCA 2 cut(s) 877, 3430
KspAI GTTAAC 2 cut(s) 2358, 3226
LguI GCTCTTC 2 cut(s) 1006, 3156
LmnI GCTCC 1 cut(s) 3019
Lsp1109I GCAGC 3 cut(s) 218, 1028, 1406
MabI ACCWGGT 1 cut(s) 2818
MaeI CTAG 7 cut(s) 95, 797, 1020, 1113, 1406, 1983, 3023
MaeII ACGT 3 cut(s) 2461, 2499, 3447
MaeIII GTNAC 9 cut(s) 113, 811, 1413, 2224, 2569, 2621, 2652, 2807, 2864
MfeI CAATTG 4 cut(s) 375, 396, 962, 3155
MflI RGATCY 7 cut(s) 331, 456, 970, 1053, 1969, 2513, 2850
MhlI GDGCHC 1 cut(s) 1360
MlsI TGGCCA 1 cut(s) 26
MluNI TGGCCA 1 cut(s) 26
MlyI GAGTC 5 cut(s) 85, 834, 2020, 3070, 3478
MmeI TCCRAC 6 cut(s) 625, 2166, 2997, 3171, 3404, 3445
Mox20I TGGCCA 1 cut(s) 26
Mph1103I ATGCAT 3 cut(s) 1450, 1704, 2414
MroI TCCGGA 1 cut(s) 3072
MroXI GAANNNNTTC 1 cut(s) 1935
MscI TGGCCA 1 cut(s) 26
MslI CAYNNNNRTG 5 cut(s) 779, 873, 1453, 2630, 3348
Msp20I TGGCCA 1 cut(s) 26
MspA1I CMGCKG 1 cut(s) 194
MspI CCGG 3 cut(s) 1714, 3053, 3073
MspR9I CCNGG 2 cut(s) 1715, 2820
MunI CAATTG 4 cut(s) 375, 396, 962, 3155
Mva1269I GAATGC 2 cut(s) 1704, 1912
MvaI CCWGG 1 cut(s) 2820
MwoI GCNNNNNNNGC 3 cut(s) 141, 1343, 2478
NciI CCSGG 1 cut(s) 1715
NcoI CCATGG 3 cut(s) 27, 858, 2644
NdeI CATATG 1 cut(s) 3041
NlaIV GGNNCC 4 cut(s) 249, 2686, 2852, 3057
NmeAIII GCCGAG 1 cut(s) 1341
NmuCI GTSAC 5 cut(s) 811, 1413, 2224, 2569, 2807
NsbI TGCGCA 1 cut(s) 1644
NsiI ATGCAT 3 cut(s) 1450, 1704, 2414
NspI RCATGY 1 cut(s) 2281
NspV TTCGAA 2 cut(s) 546, 984
PagI TCATGA 1 cut(s) 1185
PciSI GCTCTTC 2 cut(s) 1006, 3156
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PctI GAATGC 2 cut(s) 1704, 1912
PdmI GAANNNNTTC 1 cut(s) 1935
PfeI GAWTC 8 cut(s) 197, 383, 650, 897, 1232, 1274, 1924, 2101
PflFI GACNNNGTC 1 cut(s) 809
PfoI TCCNGGA 1 cut(s) 1713
PkrI GCNGC 3 cut(s) 208, 1018, 1396
PleI GAGTC 5 cut(s) 85, 833, 2019, 3070, 3477
PpsI GAGTC 5 cut(s) 85, 833, 2019, 3070, 3477
Ppu21I YACGTR 1 cut(s) 2462
PpuMI RGGWCCY 1 cut(s) 248
PsiI TTATAA 1 cut(s) 504
Psp1406I AACGTT 1 cut(s) 2499
Psp5II RGGWCCY 1 cut(s) 248
Psp6I CCWGG 1 cut(s) 2818
PspFI CCCAGC 1 cut(s) 1772
PspGI CCWGG 1 cut(s) 2818
PspN4I GGNNCC 4 cut(s) 249, 2686, 2852, 3057
PspPI GGNCC 4 cut(s) 248, 598, 2684, 3050
PspPPI RGGWCCY 1 cut(s) 248
PsuI RGATCY 7 cut(s) 331, 456, 970, 1053, 1969, 2513, 2850
PsyI GACNNNGTC 1 cut(s) 809
PvuII CAGCTG 1 cut(s) 194
RsaI GTAC 5 cut(s) 717, 1998, 2276, 2285, 2880
RsaNI GTAC 5 cut(s) 716, 1997, 2275, 2284, 2879
RseI CAYNNNNRTG 5 cut(s) 779, 873, 1453, 2630, 3348
SapI GCTCTTC 2 cut(s) 1006, 3156
SatI GCNGC 3 cut(s) 207, 1017, 1395
Sau96I GGNCC 4 cut(s) 248, 598, 2684, 3050
SchI GAGTC 5 cut(s) 85, 834, 2020, 3070, 3478
ScrFI CCNGG 2 cut(s) 1715, 2820
SduI GDGCHC 1 cut(s) 1360
SexAI ACCWGGT 1 cut(s) 2818
SfcI CTRYAG 1 cut(s) 706
SfuI TTCGAA 2 cut(s) 546, 984
SinI GGWCC 4 cut(s) 248, 598, 2684, 3050
SmiMI CAYNNNNRTG 5 cut(s) 779, 873, 1453, 2630, 3348
SmlI CTYRAG 4 cut(s) 235, 943, 2096, 2509
SmoI CTYRAG 4 cut(s) 235, 943, 2096, 2509
SnaBI TACGTA 1 cut(s) 2462
SpeI ACTAGT 1 cut(s) 1982
SsiI CCGC 1 cut(s) 831
SspI AATATT 1 cut(s) 3316
SspMI CTAG 7 cut(s) 95, 797, 1020, 1113, 1406, 1983, 3023
StyD4I CCNGG 2 cut(s) 1713, 2818
StyI CCWWGG 3 cut(s) 27, 858, 2644
TaaI ACNGT 7 cut(s) 2274, 2288, 2595, 2883, 3101, 3223, 3296
TaiI ACGT 3 cut(s) 2464, 2502, 3450
TatI WGTACW 4 cut(s) 715, 2274, 2283, 2878
TfiI GAWTC 8 cut(s) 197, 383, 650, 897, 1232, 1274, 1924, 2101
TscAI CASTG 3 cut(s) 820, 880, 3301
TseFI GTSAC 5 cut(s) 811, 1413, 2224, 2569, 2807
TseI GCWGC 3 cut(s) 206, 1016, 1394
Tsp45I GTSAC 5 cut(s) 811, 1413, 2224, 2569, 2807
TspGWI ACGGA 2 cut(s) 2740, 2843
TspRI CASTG 3 cut(s) 820, 880, 3301
Tth111I GACNNNGTC 1 cut(s) 809
VpaK11BI GGWCC 4 cut(s) 248, 598, 2684, 3050
XceI RCATGY 1 cut(s) 2281
XmiI GTMKAC 1 cut(s) 1327
XmnI GAANNNNTTC 1 cut(s) 1935
XspI CTAG 7 cut(s) 95, 797, 1020, 1113, 1406, 1983, 3023
Zsp2I ATGCAT 3 cut(s) 1450, 1704, 2414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.