pycom15g35490
WRKY Family

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000085
Physical Location & Seq
Forward (+)
35538202 .. 35541241
3040 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g35490.1

Sequence Viewer

Length: 2982 bp
ATGATTCACTATAAGAAAAACATACAGTCTCTTAAACTTCAAGCGGAAAAGCTCGAGGCAAAGAAAAATGGTTATCAAGAAATCGTTCGTGCTGCTCAAATGAATGGAGAAGATGTCAAGCCTGAGAGTCAAATATGGCTACAAGATGCTAATAAGGCCATAGCAGATGCAACACAATTAAATAATGAAGCCGAGGAAGCAGCTAAAAATTGCATCACCGGAAATTTGAGATGGCGTTACACTTTAGGAAAGAAAGTAATGAAGAAGAAAGTAGCTTTAAACAAGCTACAAGAGGAGGGAAAGTTTGAAACTATTTCAGTTTCAGTTAGGCGGCCAATTGAAATCGAGTCCACAATGTCAACGAATGGTTTTGAAGCATTTGCAGCAACAAGACAAGCCATGGATCAAGTCATGAAAGCGCTGAAAGATGATAAGGTCACCGTCATTGGGGTCTATGGAATGGGAGGTGTGGGTAAGACAACCATGGTGAAAAATGTCGGTTTGCAAGCTTGTAGAGATGGACTCTTTGATCATGTGATTATGGCTGTCTTTACCCAAAACCCCAACTTGATGAAAATTCAAGGTCAGCTTTCAGACATGTTGGGCTTGAAATTGCAGGAGGAGACAGAATTGGGAAGAGCATGTAGACTGAGGAATAGGATACTAAGAAAGAAGAGGACGCTCATAATTTTAGATGACATTTGGAATACATTATTAGATTTGATCAGCATTGGGATTCCGAACCACACGGAGCTTCAAGGATGCAATTCCAAAGTCCTTCTCACCACAAGGAGATTAAATGTCTGTCATGCCATGGAAAGCCACGTCAAAATACCTCTCAACGTCCTATCTGAAGGAGATTCCTGGAACTTGTTTGTCAAGAAAGCGGGAAAGTCTTTTGAATCGGCAGCTTTCATCAATATAGCAAGAAAGGTAGCTAGAGAATGTGCTGGTCTTCCGATAGCATTGATAGCAGTTGCAAGGGCACTTGGAGATAAAGACATTGAGGAATGGAAAGAAGCAGCTCGACAACTTGAGATGTCCCGACCTGCCAACCTTGAAGACGAGGGGGATGTGCTCAGATGCATAAAGTTAAGCTATGATTACTTGAAAGGCGAGGATGCCAAGTCATGCTTCTTGCTTTGCTCCCTATTCCCAGAAGATGGTAAAATCCCAATACAAGACTTGTTTAATTATTTGTTTGGGAATGGATTATTTCGAGATGGCAAGACACTGGAAGAAGCCAGAGCTAGAGCACATTCAGTGATCAAGTACCTTAAAGCTTCTAGCTTGCTTTTGGACGGCGAAAGGAAAGAATATGTCAGGATGCATGATGTCATACGCGATATGGCCATATCAATTGCATTATCAGAACAGGGCCACGAGTTTTTAGTAAAAGCCGGTTGGGAATTGGAGGACTGGCCAAATGATGTCGATGAAGGCTGCTCCGCAATCTCACTAAAGAGCAACTGCATTTGCAAGCTTCCGGAAGAATTGGTATGTCCAAGACTCCGAATTTTACTTGTAGATGATAATCTTAAACTAAAGAAGATCTCAGAAGCTTTTTTCCGAAGTCTGAATGCATTAAGGGTCTTAGACCTTAGCAGCACTTGTATTTCGTCACTGCCCTCTTCGCTCAATCTCCTAACCAACCTTCAAACATTGCACCTAGATTCATGTTGGTCATTAAAGGATATCTCCGTACTAAGAGAACTAACCAAACTTGAGATCCTTAGTCTGAAAGACTCGCGTTTTGAGGAATTGCCAAGGGAAATAGGAAACTTGGCCAATCTGAGGTTGTTGGATTTTGCAGGGAATACAATGATTCAGAGAGTTCCATCCAATGTGATATCTAGGCTGTCTCGGTTAGAGGAACTATACATGCAAGGTAGTTTTGGAAATTTTGGGAGTAGAATTGAAGGAGCAGGAGAAGAAACTAATGTTGGATTCGATGAGCTAACTTGCTTGTCGTCCTTAAGTAATTTGACTGTTCAGATTCATTCTGCAGAATGCATTCCCGAAAATGTTGGCTTCCCTCCAAATTGGGAAAAATTTTACATACTCATCAAGAGTCAAACAATACCCCTACATAAGGTTGAGTATTATGATTTTAGTAGTTCATATGGTTCAAGATTCTTGGCTCTTGATACCACAATCAATAACTTGCCTAGTTGGTTTAACAGTGCAGTGACCAAAAGGGCAAAGAAAATAGTCTACAAAGAATGTAGGGGCTTGATTAACATTATCGAGGAATACGCCATTGAGAGACTACATGAATTGATGTCTCTTCATGTCGAAAGTTGCCATCGGATGCTTAGTTTGATGAACTACGCGTCCACAAATTTTGTTCCGAATAAGCTCGTGTTCAAGAACTTGGAAGAGTTGTGTGTGAGTAACATGAATGAGTTAAAAGAGTTATGTGTTGGCGACTTACCAGATGGATCTCTAGGCAATCTGACGTTATTGGAAGTGAAAAAGTGTCATGCCTTTGCGAATGCACTTCTCCAATCAAATTTGTTAGAGAAACTCGGGAGTCTGGAAGTACTTATTGTTGATGGTATGAAAAGTTTGGAATACGTGTTTCAATCCGATGGACTCAAACCGGAGCAAACTGTCATGGGAAAATGCAGAGAGATGAAGCTGGAGGATCTACCAGCACTGAGAAACATATGGAATGGCCCCGCGCAACACGCGACGTTCCATAATCTTAAAATTTTGACAGTTGCATGGTGTATGAAACTGAAGAACATCTTCACGGTGGATGTGTCTCGGTGTCTTTCACAACTGGAAGAGCTGTGGCTTTCCGACTGCGATTGCTTGGAGACAATTATCGGACCGAGCGAGGGAGCATTGAACAACAAGATCATTTTGCCACAACTAAACTACTTATCGTTGCAGTGTCTTCCACGGTTCAAGAGCTTTTATAGCGGTGCAAGTACTGATGTCGAGTGCCCTTCAATGGAGCATTTGAAGTCCAAGTAA

Protein Analysis

994

Amino Acids

111.85

Weight (kDa)

6.47

Isoelectric Point (pI)

46.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 132 - 298 6.1e-30 NB-ARC domain
LRR_8 PF13855 502 - 560 1.6e-08 Leucine rich repeat
LRR_14 PF23598 565 - 668 3.4e-10 Leucine-rich repeat region
LRR_RPS2 PF23247 792 - 938 1.8e-28 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1057
AccB7I CCANNNNNTGG 1 cut(s) 1163
AccI GTMKAC 2 cut(s) 646, 2214
AccII CGCG 5 cut(s) 1344, 1750, 2333, 2684, 2693
AccIII TCCGGA 1 cut(s) 1486
AciI CCGC 6 cut(s) 44, 331, 887, 1449, 2682, 2928
AclWI GGATC 4 cut(s) 411, 1723, 2449, 2655
AcoI YGGCCR 4 cut(s) 332, 1350, 1421, 1785
AcsI RAATTY 8 cut(s) 223, 576, 1515, 1900, 2051, 2341, 2512, 2712
AcuI CTGAAG 2 cut(s) 873, 2762
AfaI GTAC 4 cut(s) 1274, 1704, 2544, 2938
AfeI AGCGCT 1 cut(s) 420
AfiI CCNNNNNNNGG 6 cut(s) 447, 1163, 1794, 2092, 2843, 2959
AflII CTTAAG 1 cut(s) 1975
AflIII ACRYGT 3 cut(s) 597, 2331, 2577
AjiI CACGTC 1 cut(s) 826
AjnI CCWGG 1 cut(s) 863
AjuI GAANNNNNNNTTGG 4 cut(s) 1118, 1150, 1926, 1958
AloI GAACNNNNNNTCC 2 cut(s) 2318, 2350
Alw21I GWGCWC 2 cut(s) 1080, 1258
Alw26I GTCTC 7 cut(s) 33, 617, 1866, 2260, 2289, 2772, 2816
AlwI GGATC 4 cut(s) 411, 1723, 2449, 2655
Ama87I CYCGRG 2 cut(s) 53, 2528
Aor13HI TCCGGA 1 cut(s) 1486
Aor51HI AGCGCT 1 cut(s) 420
AoxI GGCC 7 cut(s) 156, 332, 1350, 1378, 1421, 1785, 2677
ApeKI GCWGC 7 cut(s) 92, 200, 383, 908, 1022, 1443, 1605
ApoI RAATTY 8 cut(s) 223, 576, 1515, 1900, 2051, 2341, 2512, 2712
ArsI GACNNNNNNTTYG 2 cut(s) 1736, 1768
Asp700I GAANNNNTTC 3 cut(s) 84, 2013, 2750
AspLEI GCGC 2 cut(s) 421, 2686
AspS9I GGNCC 3 cut(s) 1378, 2678, 2834
AsuHPI GGTGA 4 cut(s) 208, 430, 499, 775
AvaI CYCGRG 2 cut(s) 53, 2528
AvaII GGWCC 1 cut(s) 2834
BaeGI GKGCMC 2 cut(s) 988, 2954
BalI TGGCCA 3 cut(s) 1352, 1423, 1787
BauI CACGAG 2 cut(s) 1382, 2360
BbsI GAAGAC 3 cut(s) 947, 1068, 2894
Bbv12I GWGCWC 2 cut(s) 1080, 1258
BbvI GCAGC 7 cut(s) 79, 212, 395, 920, 1034, 1430, 1617
BccI CCATC 9 cut(s) 225, 512, 1157, 1217, 1846, 2313, 2432, 2549, 2585
BceAI ACGGC 1 cut(s) 1318
BciT130I CCWGG 1 cut(s) 865
BciVI GTATCC 1 cut(s) 654
BclI TGATCA 3 cut(s) 529, 723, 1266
BcoDI GTCTC 7 cut(s) 33, 617, 1866, 2260, 2289, 2772, 2816
BfaI CTAG 7 cut(s) 939, 1251, 1287, 1670, 1854, 2169, 2447
BfmI CTRYAG 1 cut(s) 2004
BfoI RGCGCY 1 cut(s) 422
BfrI CTTAAG 1 cut(s) 1975
BfuAI ACCTGC 1 cut(s) 1057
BfuI GTATCC 1 cut(s) 654
BglII AGATCT 1 cut(s) 1551
BisI GCNGC 8 cut(s) 93, 201, 332, 384, 909, 1023, 1444, 1606
BlsI GCNGC 8 cut(s) 94, 202, 333, 385, 910, 1024, 1445, 1607
BmcAI AGTACT 2 cut(s) 2544, 2938
Bme1390I CCNGG 1 cut(s) 865
Bme18I GGWCC 1 cut(s) 2834
BmeT110I CYCGRG 2 cut(s) 53, 2528
BmgBI CACGTC 1 cut(s) 826
BmgT120I GGNCC 3 cut(s) 1378, 2678, 2834
BmiI GGNNCC 1 cut(s) 2680
BmrFI CCNGG 1 cut(s) 865
BmsI GCATC 8 cut(s) 136, 157, 222, 752, 1073, 1111, 1317, 2301
BpiI GAAGAC 3 cut(s) 947, 1068, 2894
BplI GAGNNNNNCTC 2 cut(s) 507, 539
BpmI CTGGAG 1 cut(s) 2663
Bpu10I CCTNAGC 1 cut(s) 1601
BpuEI CTTGAG 2 cut(s) 1055, 1745
BsaAI YACGTR 1 cut(s) 2578
BsaBI GATNNNNATC 1 cut(s) 1533
BsaJI CCNNGG 6 cut(s) 192, 399, 483, 813, 1766, 2906
BsaWI WCCGGW 3 cut(s) 218, 1486, 2602
BsaXI ACNNNNNCTCC 4 cut(s) 99, 129, 287, 317
Bsc4I CCNNNNNNNGG 6 cut(s) 447, 1163, 1794, 2092, 2843, 2959
Bse118I RCCGGY 1 cut(s) 1400
Bse1I ACTGG 3 cut(s) 1239, 1424, 2790
Bse3DI GCAATG 1 cut(s) 1661
Bse8I GATNNNNATC 1 cut(s) 1533
BseAI TCCGGA 1 cut(s) 1486
BseBI CCWGG 1 cut(s) 865
BseDI CCNNGG 6 cut(s) 192, 399, 483, 813, 1766, 2906
BseGI GGATG 7 cut(s) 767, 1078, 1126, 1332, 1838, 2316, 2767
BseJI GATNNNNATC 1 cut(s) 1533
BseLI CCNNNNNNNGG 6 cut(s) 447, 1163, 1794, 2092, 2843, 2959
BseMI GCAATG 1 cut(s) 1661
BseMII CTCAG 6 cut(s) 114, 641, 1093, 1569, 1784, 2651
BseNI ACTGG 3 cut(s) 1239, 1424, 2790
BseRI GAGGAG 2 cut(s) 308, 635
BseSI GKGCMC 2 cut(s) 988, 2954
BseXI GCAGC 7 cut(s) 79, 212, 395, 920, 1034, 1430, 1617
BsgI GTGCAG 1 cut(s) 2205
Bsh1236I CGCG 5 cut(s) 1344, 1750, 2333, 2684, 2693
BshFI GGCC 7 cut(s) 158, 334, 1352, 1380, 1423, 1787, 2679
BsiHKAI GWGCWC 2 cut(s) 1080, 1258
BsiHKCI CYCGRG 2 cut(s) 53, 2528
BsiSI CCGG 4 cut(s) 219, 1401, 1487, 2603
BslFI GGGAC 1 cut(s) 1027
BslI CCNNNNNNNGG 6 cut(s) 447, 1163, 1794, 2092, 2843, 2959
BsmAI GTCTC 7 cut(s) 33, 617, 1866, 2260, 2289, 2772, 2816
BsmFI GGGAC 1 cut(s) 1027
BsmI GAATGC 4 cut(s) 1585, 2013, 2015, 2500
BsnI GGCC 7 cut(s) 158, 334, 1352, 1380, 1423, 1787, 2679
BsoBI CYCGRG 2 cut(s) 53, 2528
Bsp1286I GDGCHC 4 cut(s) 988, 1080, 1258, 2954
Bsp13I TCCGGA 1 cut(s) 1486
Bsp143I GATC 9 cut(s) 403, 529, 723, 1266, 1551, 1728, 2441, 2647, 2862
Bsp19I CCATGG 3 cut(s) 399, 483, 813
BspACI CCGC 6 cut(s) 44, 331, 887, 1449, 2682, 2928
BspANI GGCC 7 cut(s) 158, 334, 1352, 1380, 1423, 1787, 2679
BspCNI CTCAG 6 cut(s) 115, 642, 1092, 1568, 1785, 2652
BspEI TCCGGA 1 cut(s) 1486
BspFNI CGCG 5 cut(s) 1344, 1750, 2333, 2684, 2693
BspHI TCATGA 1 cut(s) 411
BspLI GGNNCC 1 cut(s) 2680
BspMAI CTGCAG 1 cut(s) 2008
BspMI ACCTGC 1 cut(s) 1057
BspPI GGATC 4 cut(s) 411, 1723, 2449, 2655
BspQI GCTCTTC 2 cut(s) 631, 2784
BspTI CTTAAG 1 cut(s) 1975
BsrDI GCAATG 1 cut(s) 1661
BsrFI RCCGGY 1 cut(s) 1400
BsrI ACTGG 3 cut(s) 1239, 1424, 2790
BssAI RCCGGY 1 cut(s) 1400
BssECI CCNNGG 6 cut(s) 192, 399, 483, 813, 1766, 2906
BssMI GATC 9 cut(s) 403, 529, 723, 1266, 1551, 1728, 2441, 2647, 2862
BssSI CACGAG 2 cut(s) 1382, 2360
BssT1I CCWWGG 4 cut(s) 399, 483, 813, 1766
Bst2BI CACGAG 2 cut(s) 1382, 2360
Bst2UI CCWGG 1 cut(s) 865
Bst4CI ACNGT 8 cut(s) 27, 442, 1990, 2183, 2614, 2722, 2758, 2910
Bst6I CTCTTC 6 cut(s) 631, 668, 1636, 2292, 2373, 2784
BstAFI CTTAAG 1 cut(s) 1975
BstBAI YACGTR 1 cut(s) 2578
BstC8I GCNNGC 3 cut(s) 507, 1292, 1481
BstDSI CCRYGG 4 cut(s) 399, 483, 813, 2906
BstEII GGTNACC 1 cut(s) 436
BstENI CCTNNNNNAGG 1 cut(s) 2090
BstF5I GGATG 7 cut(s) 767, 1078, 1126, 1332, 1838, 2316, 2767
BstFNI CGCG 5 cut(s) 1344, 1750, 2333, 2684, 2693
BstH2I RGCGCY 1 cut(s) 422
BstHHI GCGC 2 cut(s) 421, 2686
BstKTI GATC 9 cut(s) 406, 532, 726, 1269, 1554, 1731, 2444, 2650, 2865
BstMAI GTCTC 7 cut(s) 33, 617, 1866, 2260, 2289, 2772, 2816
BstMBI GATC 9 cut(s) 403, 529, 723, 1266, 1551, 1728, 2441, 2647, 2862
BstMWI GCNNNNNNNGC 7 cut(s) 155, 197, 383, 971, 1633, 2690, 2925
BstNI CCWGG 1 cut(s) 865
BstNSI RCATGY 3 cut(s) 601, 645, 1885
BstPI GGTNACC 1 cut(s) 436
BstSCI CCNGG 1 cut(s) 863
BstSFI CTRYAG 1 cut(s) 2004
BstSLI GKGCMC 2 cut(s) 988, 2954
BstUI CGCG 5 cut(s) 1344, 1750, 2333, 2684, 2693
BstV1I GCAGC 7 cut(s) 79, 212, 395, 920, 1034, 1430, 1617
BstV2I GAAGAC 3 cut(s) 947, 1068, 2894
BstX2I RGATCY 4 cut(s) 1551, 1728, 2441, 2647
BstYI RGATCY 4 cut(s) 1551, 1728, 2441, 2647
BsuI GTATCC 1 cut(s) 654
BsuRI GGCC 7 cut(s) 158, 334, 1352, 1380, 1423, 1787, 2679
BtgI CCRYGG 4 cut(s) 399, 483, 813, 2906
BtrI CACGTC 1 cut(s) 826
BtsCI GGATG 7 cut(s) 767, 1078, 1126, 1332, 1838, 2316, 2767
BtsI GCAGTG 3 cut(s) 1622, 2193, 2903
BtsIMutI CAGTG 7 cut(s) 1232, 1269, 1622, 2188, 2193, 2657, 2903
BveI ACCTGC 1 cut(s) 1057
Cac8I GCNNGC 3 cut(s) 507, 1292, 1481
CciI TCATGA 1 cut(s) 411
CfoI GCGC 2 cut(s) 421, 2686
Cfr10I RCCGGY 1 cut(s) 1400
Cfr13I GGNCC 3 cut(s) 1378, 2678, 2834
CpoI CGGWCCG 1 cut(s) 2834
CseI GACGC 2 cut(s) 688, 2322
Csp6I GTAC 4 cut(s) 1273, 1703, 2543, 2937
CspCI CAANNNNNGTGG 2 cut(s) 2326, 2361
CspI CGGWCCG 1 cut(s) 2834
CviQI GTAC 4 cut(s) 1273, 1703, 2543, 2937
DpnI GATC 9 cut(s) 405, 531, 725, 1268, 1553, 1730, 2443, 2649, 2864
DpnII GATC 9 cut(s) 403, 529, 723, 1266, 1551, 1728, 2441, 2647, 2862
DraI TTTAAA 1 cut(s) 279
EaeI YGGCCR 4 cut(s) 332, 1350, 1421, 1785
Eam1104I CTCTTC 6 cut(s) 631, 668, 1636, 2292, 2373, 2784
EarI CTCTTC 6 cut(s) 631, 668, 1636, 2292, 2373, 2784
Eco130I CCWWGG 4 cut(s) 399, 483, 813, 1766
Eco32I GATATC 2 cut(s) 1696, 1851
Eco47I GGWCC 1 cut(s) 2834
Eco47III AGCGCT 1 cut(s) 420
Eco57I CTGAAG 2 cut(s) 873, 2762
Eco88I CYCGRG 2 cut(s) 53, 2528
Eco91I GGTNACC 1 cut(s) 436
EcoNI CCTNNNNNAGG 1 cut(s) 2090
EcoO65I GGTNACC 1 cut(s) 436
EcoRII CCWGG 1 cut(s) 863
EcoRV GATATC 2 cut(s) 1696, 1851
EcoT14I CCWWGG 4 cut(s) 399, 483, 813, 1766
EcoT22I ATGCAT 4 cut(s) 1088, 1332, 1585, 2015
ErhI CCWWGG 4 cut(s) 399, 483, 813, 1766
FalI AAGNNNNNCTT 6 cut(s) 573, 605, 1118, 1150, 2735, 2767
FaqI GGGAC 1 cut(s) 1027
FauI CCCGC 2 cut(s) 880, 2689
FauNDI CATATG 2 cut(s) 2122, 2669
FbaI TGATCA 3 cut(s) 529, 723, 1266
FblI GTMKAC 2 cut(s) 646, 2214
Fnu4HI GCNGC 8 cut(s) 93, 201, 332, 384, 909, 1023, 1444, 1606
FokI GGATG 7 cut(s) 774, 1085, 1133, 1339, 1825, 2323, 2774
Fsp4HI GCNGC 8 cut(s) 93, 201, 332, 384, 909, 1023, 1444, 1606
FspBI CTAG 7 cut(s) 939, 1251, 1287, 1670, 1854, 2169, 2447
GlaI GCGC 2 cut(s) 420, 2685
GluI GCNGC 8 cut(s) 93, 201, 332, 384, 909, 1023, 1444, 1606
GsuI CTGGAG 1 cut(s) 2663
HaeII RGCGCY 1 cut(s) 422
HaeIII GGCC 7 cut(s) 158, 334, 1352, 1380, 1423, 1787, 2679
HapII CCGG 4 cut(s) 219, 1401, 1487, 2603
HgaI GACGC 2 cut(s) 688, 2322
HhaI GCGC 2 cut(s) 421, 2686
Hin6I GCGC 2 cut(s) 419, 2684
HinP1I GCGC 2 cut(s) 419, 2684
HincII GTYRAC 1 cut(s) 360
HindII GTYRAC 1 cut(s) 360
HindIII AAGCTT 4 cut(s) 507, 1281, 1481, 1560
HpaII CCGG 4 cut(s) 219, 1401, 1487, 2603
HphI GGTGA 4 cut(s) 208, 430, 499, 775
Hpy166II GTNNAC 5 cut(s) 351, 360, 647, 2215, 2337
Hpy8I GTNNAC 5 cut(s) 351, 360, 647, 2215, 2337
Hpy99I CGWCG 1 cut(s) 2698
HpyAV CCTTC 6 cut(s) 788, 848, 1433, 1664, 1913, 2964
HpyCH4III ACNGT 8 cut(s) 27, 442, 1990, 2183, 2614, 2722, 2758, 2910
HpyCH4IV ACGT 5 cut(s) 825, 843, 2459, 2577, 2696
HpyF10VI GCNNNNNNNGC 7 cut(s) 155, 197, 383, 971, 1633, 2690, 2925
HpySE526I ACGT 5 cut(s) 825, 843, 2459, 2577, 2696
HspAI GCGC 2 cut(s) 419, 2684
Kpn2I TCCGGA 1 cut(s) 1486
Ksp22I TGATCA 3 cut(s) 529, 723, 1266
Kzo9I GATC 9 cut(s) 403, 529, 723, 1266, 1551, 1728, 2441, 2647, 2862
LguI GCTCTTC 2 cut(s) 631, 2784
LmnI GCTCC 7 cut(s) 751, 1151, 1451, 1922, 2605, 2846, 2962
Lsp1109I GCAGC 7 cut(s) 79, 212, 395, 920, 1034, 1430, 1617
LweI GCATC 8 cut(s) 136, 157, 222, 752, 1073, 1111, 1317, 2301
MaeI CTAG 7 cut(s) 939, 1251, 1287, 1670, 1854, 2169, 2447
MaeII ACGT 5 cut(s) 825, 843, 2459, 2577, 2696
MaeIII GTNAC 5 cut(s) 236, 436, 1620, 2188, 2393
MalI GATC 9 cut(s) 405, 531, 725, 1268, 1553, 1730, 2443, 2649, 2864
MboI GATC 9 cut(s) 403, 529, 723, 1266, 1551, 1728, 2441, 2647, 2862
MfeI CAATTG 2 cut(s) 336, 1359
MflI RGATCY 4 cut(s) 1551, 1728, 2441, 2647
MhlI GDGCHC 4 cut(s) 988, 1080, 1258, 2954
MlsI TGGCCA 3 cut(s) 1352, 1423, 1787
MluI ACGCGT 1 cut(s) 2331
MluNI TGGCCA 3 cut(s) 1352, 1423, 1787
MlyI GAGTC 8 cut(s) 136, 356, 516, 1503, 1739, 2080, 2542, 2589
MmeI TCCRAC 3 cut(s) 1782, 1924, 2829
Mox20I TGGCCA 3 cut(s) 1352, 1423, 1787
Mph1103I ATGCAT 4 cut(s) 1088, 1332, 1585, 2015
MroI TCCGGA 1 cut(s) 1486
MroXI GAANNNNTTC 3 cut(s) 84, 2013, 2750
MscI TGGCCA 3 cut(s) 1352, 1423, 1787
Msp20I TGGCCA 3 cut(s) 1352, 1423, 1787
MspCI CTTAAG 1 cut(s) 1975
MspI CCGG 4 cut(s) 219, 1401, 1487, 2603
MspR9I CCNGG 1 cut(s) 865
MunI CAATTG 2 cut(s) 336, 1359
Mva1269I GAATGC 4 cut(s) 1585, 2013, 2015, 2500
MvaI CCWGG 1 cut(s) 865
MvnI CGCG 5 cut(s) 1344, 1750, 2333, 2684, 2693
MwoI GCNNNNNNNGC 7 cut(s) 155, 197, 383, 971, 1633, 2690, 2925
NcoI CCATGG 3 cut(s) 399, 483, 813
NdeI CATATG 2 cut(s) 2122, 2669
NdeII GATC 9 cut(s) 403, 529, 723, 1266, 1551, 1728, 2441, 2647, 2862
NlaIV GGNNCC 1 cut(s) 2680
NmeAIII GCCGAG 1 cut(s) 217
NmuCI GTSAC 3 cut(s) 436, 1620, 2188
NsiI ATGCAT 4 cut(s) 1088, 1332, 1585, 2015
NspI RCATGY 3 cut(s) 601, 645, 1885
PaeR7I CTCGAG 1 cut(s) 53
PagI TCATGA 1 cut(s) 411
PciI ACATGT 1 cut(s) 597
PciSI GCTCTTC 2 cut(s) 631, 2784
PcsI WCGNNNNNNNCGW 1 cut(s) 2838
PctI GAATGC 4 cut(s) 1585, 2013, 2015, 2500
PdmI GAANNNNTTC 3 cut(s) 84, 2013, 2750
PfeI GAWTC 9 cut(s) 4, 736, 860, 902, 1673, 1825, 1947, 1996, 2133
PflMI CCANNNNNTGG 1 cut(s) 1163
PfoI TCCNGGA 1 cut(s) 863
PkrI GCNGC 8 cut(s) 94, 202, 333, 385, 910, 1024, 1445, 1607
PleI GAGTC 8 cut(s) 135, 355, 516, 1503, 1739, 2079, 2541, 2589
PpsI GAGTC 8 cut(s) 135, 355, 516, 1503, 1739, 2079, 2541, 2589
Ppu21I YACGTR 1 cut(s) 2578
PscI ACATGT 1 cut(s) 597
Psp6I CCWGG 1 cut(s) 863
PspEI GGTNACC 1 cut(s) 436
PspGI CCWGG 1 cut(s) 863
PspN4I GGNNCC 1 cut(s) 2680
PspPI GGNCC 3 cut(s) 1378, 2678, 2834
PspXI VCTCGAGB 1 cut(s) 53
PstI CTGCAG 1 cut(s) 2008
PsuI RGATCY 4 cut(s) 1551, 1728, 2441, 2647
RsaI GTAC 4 cut(s) 1274, 1704, 2544, 2938
RsaNI GTAC 4 cut(s) 1273, 1703, 2543, 2937
Rsr2I CGGWCCG 1 cut(s) 2834
RsrII CGGWCCG 1 cut(s) 2834
SapI GCTCTTC 2 cut(s) 631, 2784
SatI GCNGC 8 cut(s) 93, 201, 332, 384, 909, 1023, 1444, 1606
Sau3AI GATC 9 cut(s) 403, 529, 723, 1266, 1551, 1728, 2441, 2647, 2862
Sau96I GGNCC 3 cut(s) 1378, 2678, 2834
ScaI AGTACT 2 cut(s) 2544, 2938
SchI GAGTC 8 cut(s) 136, 356, 516, 1503, 1739, 2080, 2542, 2589
ScrFI CCNGG 1 cut(s) 865
SduI GDGCHC 4 cut(s) 988, 1080, 1258, 2954
SfaNI GCATC 8 cut(s) 136, 157, 222, 752, 1073, 1111, 1317, 2301
SfcI CTRYAG 1 cut(s) 2004
Sfr274I CTCGAG 1 cut(s) 53
SinI GGWCC 1 cut(s) 2834
SlaI CTCGAG 1 cut(s) 53
SmlI CTYRAG 4 cut(s) 53, 1034, 1724, 1975
SmoI CTYRAG 4 cut(s) 53, 1034, 1724, 1975
SsiI CCGC 6 cut(s) 44, 331, 887, 1449, 2682, 2928
SspMI CTAG 7 cut(s) 939, 1251, 1287, 1670, 1854, 2169, 2447
StyD4I CCNGG 1 cut(s) 863
StyI CCWWGG 4 cut(s) 399, 483, 813, 1766
TaaI ACNGT 8 cut(s) 27, 442, 1990, 2183, 2614, 2722, 2758, 2910
TaiI ACGT 5 cut(s) 828, 846, 2462, 2580, 2699
TaqI TCGA 9 cut(s) 54, 345, 1027, 1219, 1434, 1950, 2247, 2295, 2946
TaqII GACCGA 1 cut(s) 2851
TatI WGTACW 2 cut(s) 2542, 2936
TauI GCSGC 1 cut(s) 334
TfiI GAWTC 9 cut(s) 4, 736, 860, 902, 1673, 1825, 1947, 1996, 2133
TscAI CASTG 7 cut(s) 1239, 1269, 1629, 2188, 2193, 2664, 2903
TseFI GTSAC 3 cut(s) 436, 1620, 2188
TseI GCWGC 7 cut(s) 92, 200, 383, 908, 1022, 1443, 1605
Tsp45I GTSAC 3 cut(s) 436, 1620, 2188
TspGWI ACGGA 2 cut(s) 764, 1690
TspRI CASTG 7 cut(s) 1239, 1269, 1629, 2188, 2193, 2664, 2903
Van91I CCANNNNNTGG 1 cut(s) 1163
Vha464I CTTAAG 1 cut(s) 1975
VpaK11BI GGWCC 1 cut(s) 2834
XagI CCTNNNNNAGG 1 cut(s) 2090
XapI RAATTY 8 cut(s) 223, 576, 1515, 1900, 2051, 2341, 2512, 2712
XceI RCATGY 3 cut(s) 601, 645, 1885
XhoI CTCGAG 1 cut(s) 53
XmiI GTMKAC 2 cut(s) 646, 2214
XmnI GAANNNNTTC 3 cut(s) 84, 2013, 2750
XspI CTAG 7 cut(s) 939, 1251, 1287, 1670, 1854, 2169, 2447
ZrmI AGTACT 2 cut(s) 2544, 2938
Zsp2I ATGCAT 4 cut(s) 1088, 1332, 1585, 2015
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.