RchiOBHm_Chr5g0059311

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000085
Physical Location & Seq
Forward (+)
64192953 .. 64198186
5234 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33591

Sequence Viewer

Length: 3240 bp
ATGGATGACCAAGATCTTGAAGTAGGAGCTCTTATTTCTTTAAGACTCTTAAAAGCAATCGAAAAGTCAAGGCTTGCAATTGTTGTTCTCTCCCTGAATTATGCTTCTTCGAGTTGGTGTTTGGAGGAACTTACCAAGATCTGTCAATGTATGCAAGATGATGATAGAATTCTTCCGGTTTTTTATCATGTGGATCCCTCTGATGTAAGATATCAAAAGAAGAGTTTTGAAGAAGCTTTCACTAAGCATGAAAAATCTGGGCAACAAAAAATCAAGATGCAGCAGTGGAGAGATGCTTTAAATAGGGTGGCCAGTTTCTGTGGGTGGCATACACAAAATTATAAGACTGAAAGAGAGCTTGTCCATGCAATTGTGGAATCTGTGTGCAGTAAAGTCCGACCTATTAAAATTGAGTTTTCAATGTCCACAGGAGATTTTAAAGCATTTGAAGCAACAAAGCAAGCCATGAATAAGGTAATGAATGCGCTAAATGATGATGAGGTCACTGTGGTTGGGGTCTACGGAATGGGAGGTGTTGGAAAGACAACCATGGTGAAACATGTCGGTGCACAAGTGAAGAAAAGTAGACTTTTTAATCAAGTGATTATGGCTGTAGTATCACAAAACCCTGACTTGAGGAAAATTCAAGGCACATTAGCAGATTTGCTGGACTTGAAATTGGAGGATGAGACAGAAATAGGAAGAGCTAGTAGACTGGAGGAGAAGATAAAAAGAGGAATCAGGATTCTCATAATCTTGGATGACGTTTGGAATAGAATAGAATTTTCAAGCATAGGAATTCCCAGCCATCACAAACTTCAAAGATGCAATTCCAAAGTCCTACTCACCACAAGGAGATTTTCTGTATGTCATTTCATGAACAGCCATGTAAACATTCCACTCAATATCCTATCAGAAGAAGATTCTTGGAACTTGTTTGTGACAGAAGCAAGACAATCTTTTGACAAATCCACCAATTTCTATGATGTAACAAGGATGGTGGCTAGAGAATGCGCTGGTCTACCAGTTGCACTGATAGCAGTTGCGAGGGCACTTCGACATGCAAGTTTCAATGAATGGAAAGACTCGGCTCAACGACTAAAGGCGTCTCAACCTGCCAACCCTGAAGATGAGGGAGATGTGTACAAATGTATAAAGTTAAGCTATGATTACTTGAAAACTGATGATTCAAAATCATGCTTCTTGCTTTGCTGCCTGTTCCCGGAGGATTATGACATCCCAGTTGAACAGTTGCTGAAGTATGGAATTGGGAAAGGAATGTTTCAAGATTCCAACATGCAAGAAGCCAGAGCCACAACGCATTTAGTGGTCAAGTCCCTTAAAGCTTCTAGCTTGCTTCTGGACTCAAAAAAGGATGGATATGTAAAGATGCATGATGTCATTCGGGATGTGGCTGTATTAATTTCGCTATCTGAAGTTGGTCAACGGGTTTTGGTAAAAGCTGGTTGCGACTTAAAGCATTGGCCAATGATTAATGCGCATAGAGGCTTCTTTGCAATTTCACTAATGAAGAATGAAATCCGCAAGCTACCTGAAGAGTTGGTATGTCCAAACCTTAAGATTTTATTACTACAACATAATGCTAATTTAAATGACATACCCGAGACTTTTCTCCAAACCCTGAATGAACTAAGAGTCTTAGATCTTAGCCACACTAGTATTTCATTACCACCCCAATCATTCAGTCTCCTGACCAATCTCCAAGCTTTGTATTTAGATTGTTGCATGAAAATCATTGACTTTTCCATACTCGGAAAACTTGAGAAGCTTGAGATTCTTAGTATGAGGGAATATCCTCTTAAAGAATTGTCGAGAGAAATAGGACGCTTGACCAATCTAAGAATGCTGGATGTCAGTGATAGTTTCAGCCATGGACACATTGTTACAATTCCATCTAAAGTGATATCAAAGTTGCATAGACTAGAAGAACTGTACATGCATTGCAGATTTGGGGACTGGGGAAGTAAAGTCGAGGGAGGAGGAGAAGAGACTAATATTGGATTTGATGAGTTAACTGGCTTATCATATTTATACATTTTGACGGTTCGCATATGGGATGCAAAATGTTTGCCTAAATGTGTTGAGTTCAATCCAAATTGGGTTTACTTTGATATCTGTTTCGGTGGAGAAAGAAGAGTGCCGCACATTCAGTCTCAGAATGGTCATAAATATTCCAGATCCTTGATTCTTAACACGACCATCTGCAGCTTACCGGATTGGTTTGTCAGTGTGGTGACAAAGAAAACAGAGAAGCTACAGTTTGCAAAATGCGAAGGGTTAAAGAACATTCTTGTGGAATATGACCGTGGGGGGTTATGCGGGCTGAAGCATCTCTCTATTATTGGTCCCTGTGAGAAGTTGAAAGAGCTGATGAACACAATAAGTTGGATTTCAAATAAACCTGTGTTCGAGAACTTGGAGGAGTTGCACTTGGATAGTGTGGATTGCCTAAATGAATTATGTGTTGGTGAATTGCCACCTGGGTCTTTGTTTAATCTTAAGCTGTTGAATGTCTGTAATTGTCGTAACTTGGGGAATGTACTGTTGCCATCAAAACTGTTACGGAAACTACCAAATTTGGAAAAGTTGATTTGCAATGTTATGGGTAGCATGGAATATGTATTCGGGTATGAAGGGTTTGAGCCAGAAGAATCAAAATTCAGAGAGATGACATTGTTGAGTCTGTATGCTCTAAAAAGCGTATGTAATGGTCCTGCTCCCTGTACAATATTCCAGACTCTTAAAAGTTTGATCATTTACCATTGCAATCAGCTTCGAGGAAGCCTCTTCACATCTGATGTAGCTCAGTGCCTTTTTCATCTGGAAGACCTTTTTGTATTCAGTTGCCCTTTCTTGAAAAAATTAATTGAAGCACGCGAGGAAACAGTGAGCAAGAAGAAGATTGTTCTTCCAAAATTGAAGAACTTAATTTTGCTGATGCTTCCGAGGCTGTCTAGTGAAAGTGCTATTGGTATTGAGTCACCTTCATTGGTACATTTGTATGTCCAGTATTGCCCCAACTTTTCGAATGATGCTATTAACTTCCACAGCAAGAACCAAGTCCAACTCAATGATGAACGACAAATGAGGTTTCTATGGTCGAGATTGATGGGTGGTGGTCGGCGTGGAGTAAGTGGTCGGATGGGTGGTGGTCGGCGTGGAGTAAGTGGTCAGAGGCCAGATCATGGCGGCGGTATACCATGGATCGTATGGCGCTGA

Protein Analysis

1079

Amino Acids

122.61

Weight (kDa)

8.32

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR_2 PF13676 1 - 66 3.2e-07 TIR domain
TIR PF01582 2 - 134 1.7e-35 TIR domain
NB-ARC PF00931 153 - 318 8.3e-31 NB-ARC domain
LRR_8 PF13855 524 - 580 4.2e-08 Leucine rich repeat
LRR_14 PF23598 526 - 658 4.8e-13 Leucine-rich repeat region
LRR_RPS2 PF23247 813 - 956 5.2e-20 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 897 - 1015 1.3e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 342
Acc16I TGCGCA 1 cut(s) 1500
Acc36I ACCTGC 1 cut(s) 1123
AccB7I CCANNNNNTGG 1 cut(s) 3206
AccI GTMKAC 5 cut(s) 519, 586, 712, 1021, 3217
AccII CGCG 1 cut(s) 2898
AciI CCGC 5 cut(s) 1543, 2162, 2340, 3210, 3213
AclWI GGATC 4 cut(s) 188, 201, 2193, 3233
AcoI YGGCCR 2 cut(s) 309, 1484
AcsI RAATTY 6 cut(s) 168, 642, 782, 798, 2596, 2678
AcuI CTGAAG 5 cut(s) 1146, 1277, 1455, 1575, 2366
AcyI GRCGYC 1 cut(s) 1106
AfaI GTAC 5 cut(s) 1145, 1955, 2562, 2746, 3015
AfiI CCNNNNNNNGG 2 cut(s) 1222, 3206
AflII CTTAAG 2 cut(s) 1577, 2519
AflIII ACRYGT 1 cut(s) 559
AhlI ACTAGT 1 cut(s) 1676
AjnI CCWGG 1 cut(s) 2500
AjuI GAANNNNNNNTTGG 4 cut(s) 2469, 2501, 2973, 3005
Alw21I GWGCWC 2 cut(s) 31, 571
Alw26I GTCTC 6 cut(s) 683, 1113, 1619, 1712, 2003, 2178
Alw44I GTGCAC 1 cut(s) 567
AlwI GGATC 4 cut(s) 188, 201, 2193, 3233
AlwNI CAGNNNCTG 2 cut(s) 318, 1255
Ama87I CYCGRG 1 cut(s) 1622
AoxI GGCC 3 cut(s) 309, 1484, 3197
ApaLI GTGCAC 1 cut(s) 567
ApeKI GCWGC 3 cut(s) 280, 1212, 2226
ApoI RAATTY 6 cut(s) 168, 642, 782, 798, 2596, 2678
AseI ATTAAT 3 cut(s) 1421, 1494, 2885
AspLEI GCGC 4 cut(s) 487, 1016, 1501, 3237
AspS9I GGNCC 2 cut(s) 2366, 2732
AsuC2I CCSGG 1 cut(s) 1223
AsuHPI GGTGA 5 cut(s) 565, 838, 2266, 2501, 2994
AsuII TTCGAA 1 cut(s) 3047
AvaI CYCGRG 1 cut(s) 1622
AvaII GGWCC 2 cut(s) 2366, 2732
BaeGI GKGCMC 2 cut(s) 571, 1054
BaeI ACNNNNGTAYC 2 cut(s) 3005, 3038
BalI TGGCCA 2 cut(s) 311, 1486
BamHI GGATCC 1 cut(s) 193
BanII GRGCYC 1 cut(s) 31
BbsI GAAGAC 1 cut(s) 2853
Bbv12I GWGCWC 2 cut(s) 31, 571
BbvI GCAGC 3 cut(s) 292, 1199, 2238
BccI CCATC 8 cut(s) 816, 991, 1370, 1921, 2228, 2578, 3124, 3157
BciT130I CCWGG 1 cut(s) 2502
BclI TGATCA 1 cut(s) 2772
BcnI CCSGG 1 cut(s) 1223
BcoDI GTCTC 6 cut(s) 683, 1113, 1619, 1712, 2003, 2178
BcuI ACTAGT 1 cut(s) 1676
BfaI CTAG 6 cut(s) 708, 1005, 1350, 1677, 1943, 2976
BfmI CTRYAG 3 cut(s) 612, 2224, 2276
BfoI RGCGCY 1 cut(s) 3238
BfrI CTTAAG 2 cut(s) 1577, 2519
BfuAI ACCTGC 1 cut(s) 1123
BglII AGATCT 3 cut(s) 13, 138, 1663
BisI GCNGC 5 cut(s) 281, 1213, 2162, 2227, 3211
BlsI GCNGC 5 cut(s) 282, 1214, 2163, 2228, 3212
Bme1390I CCNGG 2 cut(s) 1223, 2502
Bme18I GGWCC 2 cut(s) 2366, 2732
BmeT110I CYCGRG 1 cut(s) 1622
BmgT120I GGNCC 2 cut(s) 2366, 2732
BmiI GGNNCC 2 cut(s) 195, 2368
BmrFI CCNGG 2 cut(s) 1223, 2502
BmrI ACTGGG 2 cut(s) 1235, 1987
BmsI GCATC 8 cut(s) 267, 283, 815, 1380, 2068, 2359, 2949, 3043
BmuI ACTGGG 2 cut(s) 1235, 1987
BpiI GAAGAC 1 cut(s) 2853
BplI GAGNNNNNCTC 2 cut(s) 2790, 2822
BpmI CTGGAG 1 cut(s) 737
Bpu14I TTCGAA 1 cut(s) 3047
BpuEI CTTGAG 3 cut(s) 655, 1802, 1811
BpuMI CCSGG 1 cut(s) 1223
BsaHI GRCGYC 1 cut(s) 1106
BsaJI CCNNGG 6 cut(s) 549, 1891, 2326, 2501, 2966, 3221
BsaWI WCCGGW 2 cut(s) 175, 2233
Bsc4I CCNNNNNNNGG 2 cut(s) 1222, 3206
Bse1I ACTGG 7 cut(s) 312, 720, 1025, 1241, 1982, 2041, 3028
Bse3DI GCAATG 3 cut(s) 1960, 2623, 2782
BseBI CCWGG 1 cut(s) 2502
BseDI CCNNGG 6 cut(s) 549, 1891, 2326, 2501, 2966, 3221
BseLI CCNNNNNNNGG 2 cut(s) 1222, 3206
BseMI GCAATG 3 cut(s) 1960, 2623, 2782
BseMII CTCAG 2 cut(s) 2189, 2840
BseNI ACTGG 7 cut(s) 312, 720, 1025, 1241, 1982, 2041, 3028
BseRI GAGGAG 4 cut(s) 734, 2013, 2016, 2456
BseSI GKGCMC 2 cut(s) 571, 1054
BseXI GCAGC 3 cut(s) 292, 1199, 2238
BseYI CCCAGC 1 cut(s) 803
BsgI GTGCAG 1 cut(s) 406
Bsh1236I CGCG 1 cut(s) 2898
BshFI GGCC 3 cut(s) 311, 1486, 3199
BsiHKAI GWGCWC 2 cut(s) 31, 571
BsiHKCI CYCGRG 1 cut(s) 1622
BsiSI CCGG 3 cut(s) 176, 1223, 2234
BslFI GGGAC 3 cut(s) 1321, 1988, 2352
BslI CCNNNNNNNGG 2 cut(s) 1222, 3206
BsmAI GTCTC 6 cut(s) 683, 1113, 1619, 1712, 2003, 2178
BsmBI CGTCTC 1 cut(s) 1113
BsmFI GGGAC 3 cut(s) 1321, 1988, 2352
BsmI GAATGC 3 cut(s) 487, 1016, 1869
BsnI GGCC 3 cut(s) 311, 1486, 3199
BsoBI CYCGRG 1 cut(s) 1622
Bsp119I TTCGAA 1 cut(s) 3047
Bsp1286I GDGCHC 3 cut(s) 31, 571, 1054
Bsp1407I TGTACA 3 cut(s) 1143, 1953, 2744
Bsp143I GATC 8 cut(s) 13, 138, 193, 1663, 2198, 2772, 3202, 3225
Bsp19I CCATGG 3 cut(s) 549, 1891, 3221
BspACI CCGC 5 cut(s) 1543, 2162, 2340, 3210, 3213
BspANI GGCC 3 cut(s) 311, 1486, 3199
BspCNI CTCAG 2 cut(s) 2188, 2839
BspFNI CGCG 1 cut(s) 2898
BspHI TCATGA 1 cut(s) 876
BspLI GGNNCC 2 cut(s) 195, 2368
BspMAI CTGCAG 1 cut(s) 2228
BspMI ACCTGC 1 cut(s) 1123
BspPI GGATC 4 cut(s) 188, 201, 2193, 3233
BspQI GCTCTTC 1 cut(s) 697
BspT104I TTCGAA 1 cut(s) 3047
BspTI CTTAAG 2 cut(s) 1577, 2519
BsrDI GCAATG 3 cut(s) 1960, 2623, 2782
BsrGI TGTACA 3 cut(s) 1143, 1953, 2744
BsrI ACTGG 7 cut(s) 312, 720, 1025, 1241, 1982, 2041, 3028
BssECI CCNNGG 6 cut(s) 549, 1891, 2326, 2501, 2966, 3221
BssMI GATC 8 cut(s) 13, 138, 193, 1663, 2198, 2772, 3202, 3225
BssNAI GTATAC 1 cut(s) 3218
BssNI GRCGYC 1 cut(s) 1106
BssT1I CCWWGG 3 cut(s) 549, 1891, 3221
Bst1107I GTATAC 1 cut(s) 3218
Bst2UI CCWGG 1 cut(s) 2502
Bst4CI ACNGT 9 cut(s) 508, 1251, 1953, 2065, 2280, 2327, 2565, 2580, 2908
Bst6I CTCTTC 6 cut(s) 215, 697, 1551, 2001, 2149, 2813
BstACI GRCGYC 1 cut(s) 1106
BstAFI CTTAAG 2 cut(s) 1577, 2519
BstAUI TGTACA 3 cut(s) 1143, 1953, 2744
BstBI TTCGAA 1 cut(s) 3047
BstC8I GCNNGC 6 cut(s) 75, 462, 1355, 1547, 2342, 2896
BstDEI CTNAG 8 cut(s) 243, 1652, 1660, 1667, 1799, 1859, 2175, 2826
BstDSI CCRYGG 4 cut(s) 549, 1891, 2326, 3221
BstFNI CGCG 1 cut(s) 2898
BstH2I RGCGCY 1 cut(s) 3238
BstHHI GCGC 4 cut(s) 487, 1016, 1501, 3237
BstKTI GATC 8 cut(s) 16, 141, 196, 1666, 2201, 2775, 3205, 3228
BstMAI GTCTC 6 cut(s) 683, 1113, 1619, 1712, 2003, 2178
BstMBI GATC 8 cut(s) 13, 138, 193, 1663, 2198, 2772, 3202, 3225
BstMWI GCNNNNNNNGC 3 cut(s) 449, 1037, 2968
BstNI CCWGG 1 cut(s) 2502
BstNSI RCATGY 4 cut(s) 563, 1064, 1300, 1960
BstSCI CCNGG 2 cut(s) 1221, 2500
BstSFI CTRYAG 3 cut(s) 612, 2224, 2276
BstSLI GKGCMC 2 cut(s) 571, 1054
BstUI CGCG 1 cut(s) 2898
BstV1I GCAGC 3 cut(s) 292, 1199, 2238
BstV2I GAAGAC 1 cut(s) 2853
BstX2I RGATCY 5 cut(s) 13, 138, 193, 1663, 2198
BstYI RGATCY 5 cut(s) 13, 138, 193, 1663, 2198
BstZ17I GTATAC 1 cut(s) 3218
BsuRI GGCC 3 cut(s) 311, 1486, 3199
BtgI CCRYGG 4 cut(s) 549, 1891, 2326, 3221
BtsI GCAGTG 1 cut(s) 290
BtsIMutI CAGTG 7 cut(s) 290, 504, 1031, 1882, 2254, 2834, 2913
BveI ACCTGC 1 cut(s) 1123
Cac8I GCNNGC 6 cut(s) 75, 462, 1355, 1547, 2342, 2896
CaiI CAGNNNCTG 2 cut(s) 318, 1255
CciI TCATGA 1 cut(s) 876
CfoI GCGC 4 cut(s) 487, 1016, 1501, 3237
Cfr13I GGNCC 2 cut(s) 2366, 2732
CseI GACGC 2 cut(s) 1095, 1854
Csp6I GTAC 5 cut(s) 1144, 1954, 2561, 2745, 3014
CspCI CAANNNNNGTGG 2 cut(s) 981, 1016
CviQI GTAC 5 cut(s) 1144, 1954, 2561, 2745, 3014
DdeI CTNAG 8 cut(s) 243, 1652, 1660, 1667, 1799, 1859, 2175, 2826
DpnI GATC 8 cut(s) 15, 140, 195, 1665, 2200, 2774, 3204, 3227
DpnII GATC 8 cut(s) 13, 138, 193, 1663, 2198, 2772, 3202, 3225
DraI TTTAAA 3 cut(s) 300, 439, 1611
EaeI YGGCCR 2 cut(s) 309, 1484
Eam1104I CTCTTC 6 cut(s) 215, 697, 1551, 2001, 2149, 2813
EarI CTCTTC 6 cut(s) 215, 697, 1551, 2001, 2149, 2813
Ecl136II GAGCTC 1 cut(s) 29
Eco130I CCWWGG 3 cut(s) 549, 1891, 3221
Eco24I GRGCYC 1 cut(s) 31
Eco32I GATATC 3 cut(s) 212, 1926, 2134
Eco47I GGWCC 2 cut(s) 2366, 2732
Eco53kI GAGCTC 1 cut(s) 29
Eco57I CTGAAG 5 cut(s) 1146, 1277, 1455, 1575, 2366
Eco88I CYCGRG 1 cut(s) 1622
EcoICRI GAGCTC 1 cut(s) 29
EcoRI GAATTC 2 cut(s) 168, 798
EcoRII CCWGG 1 cut(s) 2500
EcoRV GATATC 3 cut(s) 212, 1926, 2134
EcoT14I CCWWGG 3 cut(s) 549, 1891, 3221
EcoT22I ATGCAT 2 cut(s) 1395, 1962
EcoT38I GRGCYC 1 cut(s) 31
ErhI CCWWGG 3 cut(s) 549, 1891, 3221
Esp3I CGTCTC 1 cut(s) 1113
FalI AAGNNNNNCTT 2 cut(s) 943, 975
FaqI GGGAC 3 cut(s) 1321, 1988, 2352
FauI CCCGC 1 cut(s) 2333
FauNDI CATATG 1 cut(s) 2072
FbaI TGATCA 1 cut(s) 2772
FblI GTMKAC 5 cut(s) 519, 586, 712, 1021, 3217
Fnu4HI GCNGC 5 cut(s) 281, 1213, 2162, 2227, 3211
FriOI GRGCYC 1 cut(s) 31
Fsp4HI GCNGC 5 cut(s) 281, 1213, 2162, 2227, 3211
FspAI RTGCGCAY 1 cut(s) 1500
FspBI CTAG 6 cut(s) 708, 1005, 1350, 1677, 1943, 2976
FspI TGCGCA 1 cut(s) 1500
GlaI GCGC 4 cut(s) 486, 1015, 1500, 3236
GluI GCNGC 5 cut(s) 281, 1213, 2162, 2227, 3211
GsaI CCCAGC 1 cut(s) 807
GsuI CTGGAG 1 cut(s) 737
HaeII RGCGCY 1 cut(s) 3238
HaeIII GGCC 3 cut(s) 311, 1486, 3199
HapII CCGG 3 cut(s) 176, 1223, 2234
HgaI GACGC 2 cut(s) 1095, 1854
HhaI GCGC 4 cut(s) 487, 1016, 1501, 3237
Hin1I GRCGYC 1 cut(s) 1106
Hin6I GCGC 4 cut(s) 485, 1014, 1499, 3235
HinP1I GCGC 4 cut(s) 485, 1014, 1499, 3235
HincII GTYRAC 2 cut(s) 1445, 2034
HindII GTYRAC 2 cut(s) 1445, 2034
HindIII AAGCTT 4 cut(s) 234, 1344, 1725, 1787
HpaI GTTAAC 1 cut(s) 2034
HpaII CCGG 3 cut(s) 176, 1223, 2234
HphI GGTGA 5 cut(s) 565, 838, 2266, 2501, 2994
HpyAV CCTTC 3 cut(s) 2288, 2648, 3015
HpyCH4III ACNGT 9 cut(s) 508, 1251, 1953, 2065, 2280, 2327, 2565, 2580, 2908
HpyCH4IV ACGT 1 cut(s) 765
HpyF10VI GCNNNNNNNGC 3 cut(s) 449, 1037, 2968
HpyF3I CTNAG 8 cut(s) 243, 1652, 1660, 1667, 1799, 1859, 2175, 2826
HpySE526I ACGT 1 cut(s) 765
Hsp92I GRCGYC 1 cut(s) 1106
HspAI GCGC 4 cut(s) 485, 1014, 1499, 3235
Ksp22I TGATCA 1 cut(s) 2772
KspAI GTTAAC 1 cut(s) 2034
Kzo9I GATC 8 cut(s) 13, 138, 193, 1663, 2198, 2772, 3202, 3225
LguI GCTCTTC 1 cut(s) 697
LmnI GCTCC 2 cut(s) 26, 2743
Lsp1109I GCAGC 3 cut(s) 292, 1199, 2238
LweI GCATC 8 cut(s) 267, 283, 815, 1380, 2068, 2359, 2949, 3043
MaeI CTAG 6 cut(s) 708, 1005, 1350, 1677, 1943, 2976
MaeII ACGT 1 cut(s) 765
MaeIII GTNAC 8 cut(s) 502, 940, 988, 1903, 2254, 2546, 2580, 3000
MalI GATC 8 cut(s) 15, 140, 195, 1665, 2200, 2774, 3204, 3227
MboI GATC 8 cut(s) 13, 138, 193, 1663, 2198, 2772, 3202, 3225
MfeI CAATTG 2 cut(s) 78, 369
MflI RGATCY 5 cut(s) 13, 138, 193, 1663, 2198
MhlI GDGCHC 3 cut(s) 31, 571, 1054
MlsI TGGCCA 2 cut(s) 311, 1486
MluNI TGGCCA 2 cut(s) 311, 1486
MlyI GAGTC 7 cut(s) 39, 1079, 1358, 1665, 2710, 2752, 3008
MmeI TCCRAC 6 cut(s) 421, 517, 1317, 2387, 3109, 3140
Mox20I TGGCCA 2 cut(s) 311, 1486
Mph1103I ATGCAT 2 cut(s) 1395, 1962
MscI TGGCCA 2 cut(s) 311, 1486
MslI CAYNNNNRTG 3 cut(s) 564, 2312, 3021
Msp20I TGGCCA 2 cut(s) 311, 1486
MspCI CTTAAG 2 cut(s) 1577, 2519
MspI CCGG 3 cut(s) 176, 1223, 2234
MspR9I CCNGG 2 cut(s) 1223, 2502
MunI CAATTG 2 cut(s) 78, 369
Mva1269I GAATGC 3 cut(s) 487, 1016, 1869
MvaI CCWGG 1 cut(s) 2502
MvnI CGCG 1 cut(s) 2898
MwoI GCNNNNNNNGC 3 cut(s) 449, 1037, 2968
NciI CCSGG 1 cut(s) 1223
NcoI CCATGG 3 cut(s) 549, 1891, 3221
NdeI CATATG 1 cut(s) 2072
NdeII GATC 8 cut(s) 13, 138, 193, 1663, 2198, 2772, 3202, 3225
NlaIV GGNNCC 2 cut(s) 195, 2368
NmeAIII GCCGAG 1 cut(s) 1067
NmuCI GTSAC 4 cut(s) 502, 940, 2254, 3000
NsbI TGCGCA 1 cut(s) 1500
NsiI ATGCAT 2 cut(s) 1395, 1962
NspI RCATGY 4 cut(s) 563, 1064, 1300, 1960
NspV TTCGAA 1 cut(s) 3047
PagI TCATGA 1 cut(s) 876
PciI ACATGT 1 cut(s) 559
PciSI GCTCTTC 1 cut(s) 697
PctI GAATGC 3 cut(s) 487, 1016, 1869
PfeI GAWTC 9 cut(s) 377, 738, 745, 923, 1187, 1289, 1795, 2206, 2672
PflMI CCANNNNNTGG 1 cut(s) 3206
PfoI TCCNGGA 1 cut(s) 1221
PkrI GCNGC 5 cut(s) 282, 1214, 2163, 2228, 3212
PleI GAGTC 7 cut(s) 39, 1079, 1358, 1664, 2709, 2752, 3007
PpsI GAGTC 7 cut(s) 39, 1079, 1358, 1664, 2709, 2752, 3007
PscI ACATGT 1 cut(s) 559
PshBI ATTAAT 3 cut(s) 1421, 1494, 2885
PsiI TTATAA 1 cut(s) 342
Psp124BI GAGCTC 1 cut(s) 31
Psp6I CCWGG 1 cut(s) 2500
PspFI CCCAGC 1 cut(s) 803
PspGI CCWGG 1 cut(s) 2500
PspN4I GGNNCC 2 cut(s) 195, 2368
PspPI GGNCC 2 cut(s) 2366, 2732
PstI CTGCAG 1 cut(s) 2228
PstNI CAGNNNCTG 2 cut(s) 318, 1255
PsuI RGATCY 5 cut(s) 13, 138, 193, 1663, 2198
RsaI GTAC 5 cut(s) 1145, 1955, 2562, 2746, 3015
RsaNI GTAC 5 cut(s) 1144, 1954, 2561, 2745, 3014
RseI CAYNNNNRTG 3 cut(s) 564, 2312, 3021
SacI GAGCTC 1 cut(s) 31
SapI GCTCTTC 1 cut(s) 697
SatI GCNGC 5 cut(s) 281, 1213, 2162, 2227, 3211
Sau3AI GATC 8 cut(s) 13, 138, 193, 1663, 2198, 2772, 3202, 3225
Sau96I GGNCC 2 cut(s) 2366, 2732
SchI GAGTC 7 cut(s) 39, 1079, 1358, 1665, 2710, 2752, 3008
ScrFI CCNGG 2 cut(s) 1223, 2502
SduI GDGCHC 3 cut(s) 31, 571, 1054
SfaNI GCATC 8 cut(s) 267, 283, 815, 1380, 2068, 2359, 2949, 3043
SfcI CTRYAG 3 cut(s) 612, 2224, 2276
SfuI TTCGAA 1 cut(s) 3047
SinI GGWCC 2 cut(s) 2366, 2732
SmiI ATTTAAAT 1 cut(s) 1611
SmiMI CAYNNNNRTG 3 cut(s) 564, 2312, 3021
SmlI CTYRAG 5 cut(s) 634, 1577, 1781, 1790, 2519
SmoI CTYRAG 5 cut(s) 634, 1577, 1781, 1790, 2519
SpeI ACTAGT 1 cut(s) 1676
SsiI CCGC 5 cut(s) 1543, 2162, 2340, 3210, 3213
SspI AATATT 3 cut(s) 2017, 2192, 2751
SspMI CTAG 6 cut(s) 708, 1005, 1350, 1677, 1943, 2976
SstI GAGCTC 1 cut(s) 31
StyD4I CCNGG 2 cut(s) 1221, 2500
StyI CCWWGG 3 cut(s) 549, 1891, 3221
SwaI ATTTAAAT 1 cut(s) 1611
TaaI ACNGT 9 cut(s) 508, 1251, 1953, 2065, 2280, 2327, 2565, 2580, 2908
TaiI ACGT 1 cut(s) 768
TaqI TCGA 9 cut(s) 60, 110, 1057, 1832, 1992, 2430, 2797, 3047, 3122
TatI WGTACW 4 cut(s) 1143, 1953, 2560, 2744
TauI GCSGC 2 cut(s) 2164, 3213
TfiI GAWTC 9 cut(s) 377, 738, 745, 923, 1187, 1289, 1795, 2206, 2672
TscAI CASTG 7 cut(s) 290, 511, 1038, 1882, 2254, 2834, 2913
TseFI GTSAC 4 cut(s) 502, 940, 2254, 3000
TseI GCWGC 3 cut(s) 280, 1212, 2226
Tsp45I GTSAC 4 cut(s) 502, 940, 2254, 3000
TspGWI ACGGA 2 cut(s) 537, 2599
TspRI CASTG 7 cut(s) 290, 511, 1038, 1882, 2254, 2834, 2913
Van91I CCANNNNNTGG 1 cut(s) 3206
Vha464I CTTAAG 2 cut(s) 1577, 2519
VneI GTGCAC 1 cut(s) 567
VpaK11BI GGWCC 2 cut(s) 2366, 2732
VspI ATTAAT 3 cut(s) 1421, 1494, 2885
XapI RAATTY 6 cut(s) 168, 642, 782, 798, 2596, 2678
XceI RCATGY 4 cut(s) 563, 1064, 1300, 1960
XcmI CCANNNNNNNNNTGG 1 cut(s) 3228
XmiI GTMKAC 5 cut(s) 519, 586, 712, 1021, 3217
XspI CTAG 6 cut(s) 708, 1005, 1350, 1677, 1943, 2976
Zsp2I ATGCAT 2 cut(s) 1395, 1962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.