RchiOBHm_Chr3g0473571

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000085
Physical Location & Seq
Forward (+)
19380296 .. 19386190
5895 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43923

Sequence Viewer

Length: 3471 bp
ATGGTTGTAAAGCCTGAGCCAATGTCGGAGGATAATCAAGAACCGGTTGATGTTGATGCCTCTGCATCTCTTCCTTCATCATCAGCTCCTCGGTGGAAGCATGATGTGTTTTTGAGTTTCAGGGGTGCAGACGCTCGCAAGGGTATTGCATTTGAGTTATACGATCGACTGCAGAACAGGAGAGGAATCAAAACATTCATGGATGACCATGACCTTCAAGTAGGGGATGTTATTTCTCCCACTCTCCTAACGGCAATTAAAGAATCAAGGTTTGCAATTATTGTTCTCTCTCAAAACTACGCCTCTTCCACTTGGTGTTTGGAGGAACTTAGAAACATCTGTGAGTGCATGGAAGAAGACAACAATAGAATTCTGCCACTTTTTTATTATGTGGATCCTACTGATGTACGATATCAGAAGAGGAGTTTCGGAGATGCTTTTACTAGGCATGAAAAATCACAGCGACACAGATCAGAGAAGGTGCAGCAGTGGAGAGATGCTTTAAAAAAAGTGGCAAATTTCTCTGGATGGCATACACTGAATTATAAAACTGAAAGAGAACTTTTGGATGCCATTGAGGAATCTGTGTCCAATAAATTACGACCTACTGAACTTGAGTTTAAAATGTCCATTGGAGGTTTTGAAGAATTTGCAGCAACAAAACAAGCCATTGATGAGGTTATGAATGCACTAAAAGATGATGAGGCCACTACCATTGGAGTCTACAGAATGGGGGGCGTTGGAAAGACGACAATGGTGAAATATGTGGGAGCACAAGCCCAAAAAAGTAGGCTTTTTAATCAAGTGATTATGGCTGTCGTATCCCAAAACCCCGACTTGATGAAAATTCAAGAGACATTTGCCGAACTGCTGGGCTTTAAATTGGAGGAGAAGACAGAAATTGGAAGATCCATTAAATTGAAGGATAAGATAATGAGAGGAACTGGGATCCTCATAATCTTGGATGACATTTGGAAGAGAATAGACTTTTCAGACATTGGAATTCCGAGCCACAACGAACTTCAAAAATGCAATTCCAAAGTCCTACTGACCACCAGAAAATCGAGAGTTTGTAATACCATGAAGAGCCATGCAAAAATTCATCTCAATACCTTATCAGAAGAAGATGCTCGGAGCTTATTTGTGAAGGAAGCAGGGAAGTCTTTTGACAAATCTTCCAATTTCTATGATGTAGCGAGTGAGGTGGCAAGAGAATGCGCCGGTCTACCGGTTGCTTTGGTAGCAGTTGCGAGGGCCCTTCGAGATGAAGGTTTGGACGGATGGAAACAAGCTGCTCGACGACTAAAAGCGTCTCAACCTCCCATCCCTGAAGATGAGGGCGATGTGTTCAAATGCATAAAGTTAAGCTATGATTACTTGAAATCTAATGATTCCAAATCATGCTTCTTGCTTTGCTGCCTGTTCCCAGAAGATTATGATATCCCAATTGACTACCTGTTCAAGTATGGAGTTGGGAAAGGGATGTTTCAAGATTCAAACTTGCTAGATGCCCGAGCCACAACATATTTAGTGGTGAAGGCCCTTAAAGATTCTAGCTTGCTTTTGGACGCTGAAGATGACAGATGCGTAAAGATGCATGATGTCATTCGGGATATGGCAATCTCAATTTCGTTATCGGAAGGCCAGCGGGTCTTGGTGAAAGCTGGCTGTGAATTGAAGAATTGGCCACAGATTGATGCACATAAAGGCTACTCTGGAATCTCACTAATGGTGAACAAAATTTGCAAGCTACCCCAAGAGTTGGTATGTCCAAATCTCCAGATTTTATTACTGCAACACAATGCTTCATTAAATGATATCCCAAACTCTTTCTTCCAAAGTCAGAAAGAATTAAGGGTCTTAGATCTTAGCCGCACTGGTATTTCATTACTACCGCAATCAATCAATTTCCTAACCAATCTTCAAGCTTTGTATTTAGATCATTGCAACAGCATAATTGACATTTCTGTAATCGGAAAACTTAACAAGCTTGAGATTCTTAGTATGAGAGGAATTGCTCTGAGAGAATTTCCAAGAGAAATAGGACAATTGACCAATCTAAGGATGTTAGATGTCAGTGCTGAATTTAATCCACGGACACAGAGATTTGATGGACGTATTGCCACACTTCCATCTGAAGTGATATCAAAGTTGCATAGATTAGAAGAACTGTACATGCAATGTACATTTTCGGACTGGAGGAGTAACATTGATGGAGAAGGGGATGGATTCTCCGAGAGCTTGGAGAGTGATACTGATGGAGAAGGGGAAGAAACTAATATTGGCTTTGATGAATTAGTTGGTTTATCATATTTAAGCATTCTGAAAGTTTGCATATCAGATGGAAATTGCATCCCTAGAAGTATTGAGGTTGAACCGAATTGGGTTTACTTCAGTATTAGTATTGGCAGCTGCAATGATATAGCGAGACATAGTAAGAACATTCCATCCGGTCATAATTTTAGATCCTTGAGTCTTGATAGTACTGACACAACCATAGGAACCTTTCCAGACTGGTTTGTCAACGCTGTTGTAAAGAATACTGAGGGGCTACAGTATAAAGACTGCAGAGGGTTAAGTAACATTCTTGTGGAATATGACCATGGGAGGTTACATGGACTGAAAGTTCTCTCTGTAATTGGTCGCTGTGAGAACCTGAAAGAGTTGATGAACGCCATAACATGTGTTCCTGATAAGCCTGTGTTTGAGAACATGGAAGAGTTGCATCTGCAAGAGCTGAATGACCTGGAGGAGTTATGTGTTGGTGAGTTACCAGCTGGATCTCTCTGGAGTCTGAAATTACTGAAGGTCAGTCGGTGTCCTAATTTGGGAAATGTACTGTTGCCATCAAAATTGTTGCAGAAACTACCAAATCTGGAAAAACTAATCTGTGAGACAAGTGGAGTGGAATATATTTTTGGATGTGAAGGATTTGAGCCGGATCAAACAAATCTGAGAGAGATGGAGTTATGGGATCTAGATGTAATAAGAAGCATATGTAATGGTCCTGCCCCACGTGGAATGTTCCAGACTCTAAAGGAATTGGCCATATATAATTGCAACTTCCAGGGAAGCCTCTTCACATTTGATGTCGCTCAGTGTCTTTTTCAATTGGAAAAGCTTACTGTAGTCAAATGCCCTCTGTTGGAAGGAGTAATCGAAGCAAGGAGGGAAACATTGAACAACAAGAAGACCGTTCTTCCAAAATTGAAGAACTTACGTTTGCATAGTCTTCCAATGTTGTACAAGGGAAGTGCTACTATTGATTTTGAGTGTCCTTCATTGGAAGACTTGCATCTGCAGGACTGCCCCCACTTGTCATTTCCATCCTCTGCTTCTGCTTCTGACTACTTCCACAGCAGGAACCAAGTCCTTTTCAATGATAGGCGTCATCGCGTTGATTTAATAACTATACGGGCAGCCAATTTATTTATGTACAAAATGAATGCACCGAAGTTGAAGAACACTTGA

Protein Analysis

1156

Amino Acids

130.74

Weight (kDa)

5.7

Isoelectric Point (pI)

44.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 33 - 204 2.9e-51 TIR domain
TIR_2 PF13676 36 - 147 8.3e-17 TIR domain
NB-ARC PF00931 221 - 387 1.2e-26 NB-ARC domain
LRR_8 PF13855 591 - 650 2e-08 Leucine rich repeat
LRR_14 PF23598 615 - 731 9.9e-14 Leucine-rich repeat region
LRR_RPS2 PF23247 905 - 1046 1.3e-21 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 999 - 1110 2.5e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 546
AasI GACNNNNNNGTC 1 cut(s) 2519
AccB7I CCANNNNNTGG 1 cut(s) 1762
AccI GTMKAC 2 cut(s) 723, 1225
AccII CGCG 1 cut(s) 3396
AciI CCGC 3 cut(s) 1648, 1873, 1895
AclWI GGATC 9 cut(s) 389, 402, 903, 943, 956, 2460, 2788, 2949, 2982
AcoI YGGCCR 2 cut(s) 1685, 3045
AcsI RAATTY 9 cut(s) 369, 517, 647, 846, 1002, 1098, 1740, 2027, 2084
AcuI CTGAAG 5 cut(s) 1350, 1593, 2157, 2377, 2825
AcvI CACGTG 1 cut(s) 3017
AcyI GRCGYC 1 cut(s) 3388
AdeI CACNNNGTG 1 cut(s) 315
AfaI GTAC 7 cut(s) 408, 2174, 2185, 2485, 2838, 3245, 3437
AfiI CCNNNNNNNGG 4 cut(s) 1762, 2061, 2828, 3145
AflIII ACRYGT 1 cut(s) 2681
AgeI ACCGGT 2 cut(s) 43, 1228
AjnI CCWGG 2 cut(s) 2745, 3066
AjuI GAANNNNNNNTTGG 6 cut(s) 1388, 1420, 2265, 2297, 2901, 2933
AloI GAACNNNNNNTCC 4 cut(s) 2432, 2464, 2610, 2642
Alw21I GWGCWC 1 cut(s) 775
Alw26I GTCTC 4 cut(s) 848, 1317, 2422, 2888
AlwI GGATC 9 cut(s) 389, 402, 903, 943, 956, 2460, 2788, 2949, 2982
Ama87I CYCGRG 1 cut(s) 1512
AoxI GGCC 6 cut(s) 705, 1254, 1539, 1642, 1685, 3045
ApaI GGGCCC 1 cut(s) 1258
ApeKI GCWGC 7 cut(s) 484, 653, 1292, 1416, 2409, 2412, 3419
ApoI RAATTY 9 cut(s) 369, 517, 647, 846, 1002, 1098, 1740, 2027, 2084
AsiGI ACCGGT 2 cut(s) 43, 1228
AspLEI GCGC 1 cut(s) 1220
AspS9I GGNCC 4 cut(s) 1254, 1255, 1540, 3005
AsuHPI GGTGA 5 cut(s) 769, 1546, 1669, 1744, 2777
AvaI CYCGRG 1 cut(s) 1512
AvaII GGWCC 1 cut(s) 3005
BaeGI GKGCMC 1 cut(s) 1258
BalI TGGCCA 2 cut(s) 1687, 3047
BamHI GGATCC 2 cut(s) 394, 948
BanII GRGCYC 1 cut(s) 1258
BbrPI CACGTG 1 cut(s) 3017
BbsI GAAGAC 5 cut(s) 363, 899, 3197, 3224, 3294
Bbv12I GWGCWC 1 cut(s) 775
BbvI GCAGC 7 cut(s) 496, 665, 1279, 1403, 2399, 2421, 3431
BceAI ACGGC 1 cut(s) 267
BciT130I CCWGG 2 cut(s) 2747, 3068
BciVI GTATCC 1 cut(s) 832
BcoDI GTCTC 4 cut(s) 848, 1317, 2422, 2888
BfaI CTAG 5 cut(s) 444, 1505, 1554, 2358, 2978
BfmI CTRYAG 6 cut(s) 170, 724, 2552, 2566, 3126, 3299
BfuI GTATCC 1 cut(s) 832
BglII AGATCT 1 cut(s) 1864
BisI GCNGC 8 cut(s) 485, 654, 1293, 1417, 1873, 2410, 2413, 3420
BlsI GCNGC 8 cut(s) 486, 655, 1294, 1418, 1874, 2411, 2414, 3421
BmcAI AGTACT 1 cut(s) 2485
Bme1390I CCNGG 2 cut(s) 2747, 3068
Bme18I GGWCC 1 cut(s) 3005
BmeT110I CYCGRG 1 cut(s) 1512
BmgT120I GGNCC 4 cut(s) 1254, 1255, 1540, 3005
BmiI GGNNCC 5 cut(s) 396, 950, 1256, 2503, 3365
BmrFI CCNGG 2 cut(s) 2747, 3068
BmrI ACTGGG 1 cut(s) 954
BmuI ACTGGG 1 cut(s) 954
BpiI GAAGAC 5 cut(s) 363, 899, 3197, 3224, 3294
BpmI CTGGAG 4 cut(s) 1763, 2218, 2768, 2809
Bpu10I CCTNAGC 1 cut(s) 15
BpuEI CTTGAG 3 cut(s) 635, 2012, 2491
BsaAI YACGTR 1 cut(s) 3017
BsaHI GRCGYC 1 cut(s) 3388
BsaJI CCNNGG 4 cut(s) 89, 2093, 2602, 3067
BsaWI WCCGGW 3 cut(s) 43, 1228, 2450
Bsc4I CCNNNNNNNGG 4 cut(s) 1762, 2061, 2828, 3145
Bse118I RCCGGY 3 cut(s) 43, 1220, 1228
Bse1I ACTGG 4 cut(s) 949, 1882, 2201, 2519
Bse3DI GCAATG 3 cut(s) 1942, 2186, 2422
BseBI CCWGG 2 cut(s) 2747, 3068
BseDI CCNNGG 4 cut(s) 89, 2093, 2602, 3067
BseLI CCNNNNNNNGG 4 cut(s) 1762, 2061, 2828, 3145
BseMI GCAATG 3 cut(s) 1942, 2186, 2422
BseMII CTCAG 5 cut(s) 6, 2012, 2535, 2945, 3110
BseNI ACTGG 4 cut(s) 949, 1882, 2201, 2519
BseRI GAGGAG 5 cut(s) 78, 436, 902, 2215, 2765
BseSI GKGCMC 1 cut(s) 1258
BseXI GCAGC 7 cut(s) 496, 665, 1279, 1403, 2399, 2421, 3431
BseYI CCCAGC 1 cut(s) 871
BsgI GTGCAG 2 cut(s) 147, 503
Bsh1236I CGCG 1 cut(s) 3396
Bsh1285I CGRYCG 1 cut(s) 166
BshFI GGCC 6 cut(s) 707, 1256, 1541, 1644, 1687, 3047
BshTI ACCGGT 2 cut(s) 43, 1228
BsiEI CGRYCG 1 cut(s) 166
BsiHKAI GWGCWC 1 cut(s) 775
BsiHKCI CYCGRG 1 cut(s) 1512
BsiSI CCGG 5 cut(s) 44, 1221, 1229, 2451, 2939
BslI CCNNNNNNNGG 4 cut(s) 1762, 2061, 2828, 3145
BsmAI GTCTC 4 cut(s) 848, 1317, 2422, 2888
BsmBI CGTCTC 1 cut(s) 1317
BsmI GAATGC 4 cut(s) 691, 1220, 2319, 3451
BsnI GGCC 6 cut(s) 707, 1256, 1541, 1644, 1687, 3047
BsoBI CYCGRG 1 cut(s) 1512
Bsp120I GGGCCC 1 cut(s) 1254
Bsp1286I GDGCHC 2 cut(s) 775, 1258
Bsp1407I TGTACA 4 cut(s) 2172, 2183, 3243, 3435
Bsp19I CCATGG 1 cut(s) 2602
BspACI CCGC 3 cut(s) 1648, 1873, 1895
BspANI GGCC 6 cut(s) 707, 1256, 1541, 1644, 1687, 3047
BspCNI CTCAG 5 cut(s) 7, 2013, 2536, 2946, 3109
BspFNI CGCG 1 cut(s) 3396
BspLI GGNNCC 5 cut(s) 396, 950, 1256, 2503, 3365
BspMAI CTGCAG 3 cut(s) 174, 2570, 3303
BspPI GGATC 9 cut(s) 389, 402, 903, 943, 956, 2460, 2788, 2949, 2982
BspQI GCTCTTC 1 cut(s) 1079
BsrDI GCAATG 3 cut(s) 1942, 2186, 2422
BsrFI RCCGGY 3 cut(s) 43, 1220, 1228
BsrGI TGTACA 4 cut(s) 2172, 2183, 3243, 3435
BsrI ACTGG 4 cut(s) 949, 1882, 2201, 2519
BssAI RCCGGY 3 cut(s) 43, 1220, 1228
BssECI CCNNGG 4 cut(s) 89, 2093, 2602, 3067
BssNI GRCGYC 1 cut(s) 3388
BssT1I CCWWGG 1 cut(s) 2602
Bst2UI CCWGG 2 cut(s) 2747, 3068
Bst4CI ACNGT 5 cut(s) 2172, 2556, 2841, 3127, 3196
Bst6I CTCTTC 7 cut(s) 75, 310, 413, 971, 1079, 2712, 3083
BstACI GRCGYC 1 cut(s) 3388
BstAUI TGTACA 4 cut(s) 2172, 2183, 3243, 3435
BstBAI YACGTR 1 cut(s) 3017
BstC8I GCNNGC 5 cut(s) 136, 1559, 1646, 1666, 1748
BstDSI CCRYGG 2 cut(s) 2093, 2602
BstFNI CGCG 1 cut(s) 3396
BstHHI GCGC 1 cut(s) 1220
BstMAI GTCTC 4 cut(s) 848, 1317, 2422, 2888
BstMCI CGRYCG 1 cut(s) 166
BstMWI GCNNNNNNNGC 1 cut(s) 1241
BstNI CCWGG 2 cut(s) 2747, 3068
BstNSI RCATGY 2 cut(s) 2179, 2685
BstSCI CCNGG 2 cut(s) 2745, 3066
BstSFI CTRYAG 6 cut(s) 170, 724, 2552, 2566, 3126, 3299
BstSLI GKGCMC 1 cut(s) 1258
BstUI CGCG 1 cut(s) 3396
BstV1I GCAGC 7 cut(s) 496, 665, 1279, 1403, 2399, 2421, 3431
BstV2I GAAGAC 5 cut(s) 363, 899, 3197, 3224, 3294
BstX2I RGATCY 7 cut(s) 394, 908, 948, 1864, 2465, 2780, 2974
BstYI RGATCY 7 cut(s) 394, 908, 948, 1864, 2465, 2780, 2974
BsuI GTATCC 1 cut(s) 832
BsuRI GGCC 6 cut(s) 707, 1256, 1541, 1644, 1687, 3047
BtgI CCRYGG 2 cut(s) 2093, 2602
BtgZI GCGATG 2 cut(s) 1356, 3377
BtsI GCAGTG 1 cut(s) 494
BtsIMutI CAGTG 5 cut(s) 494, 536, 1875, 2083, 3104
Cac8I GCNNGC 5 cut(s) 136, 1559, 1646, 1666, 1748
CfoI GCGC 1 cut(s) 1220
Cfr10I RCCGGY 3 cut(s) 43, 1220, 1228
Cfr13I GGNCC 4 cut(s) 1254, 1255, 1540, 3005
CseI GACGC 4 cut(s) 140, 1299, 1577, 3377
Csp6I GTAC 7 cut(s) 407, 2173, 2184, 2484, 2837, 3244, 3436
CspAI ACCGGT 2 cut(s) 43, 1228
CspCI CAANNNNNGTGG 4 cut(s) 697, 732, 2886, 2921
CviQI GTAC 7 cut(s) 407, 2173, 2184, 2484, 2837, 3244, 3436
DraI TTTAAA 3 cut(s) 504, 622, 880
DraIII CACNNNGTG 1 cut(s) 315
DrdI GACNNNNNNGTC 1 cut(s) 2519
DseDI GACNNNNNNGTC 1 cut(s) 2519
EaeI YGGCCR 2 cut(s) 1685, 3045
Eam1104I CTCTTC 7 cut(s) 75, 310, 413, 971, 1079, 2712, 3083
EarI CTCTTC 7 cut(s) 75, 310, 413, 971, 1079, 2712, 3083
Eco130I CCWWGG 1 cut(s) 2602
Eco24I GRGCYC 1 cut(s) 1258
Eco32I GATATC 4 cut(s) 413, 1441, 1819, 2145
Eco47I GGWCC 1 cut(s) 3005
Eco57I CTGAAG 5 cut(s) 1350, 1593, 2157, 2377, 2825
Eco72I CACGTG 1 cut(s) 3017
Eco88I CYCGRG 1 cut(s) 1512
EcoO109I RGGNCCY 3 cut(s) 1254, 1255, 1540
EcoRI GAATTC 2 cut(s) 369, 1002
EcoRII CCWGG 2 cut(s) 2745, 3066
EcoRV GATATC 4 cut(s) 413, 1441, 1819, 2145
EcoT14I CCWWGG 1 cut(s) 2602
EcoT22I ATGCAT 2 cut(s) 1358, 1599
EcoT38I GRGCYC 1 cut(s) 1258
ErhI CCWWGG 1 cut(s) 2602
Esp3I CGTCTC 1 cut(s) 1317
FauI CCCGC 1 cut(s) 1641
FauNDI CATATG 1 cut(s) 2996
FblI GTMKAC 2 cut(s) 723, 1225
Fnu4HI GCNGC 8 cut(s) 485, 654, 1293, 1417, 1873, 2410, 2413, 3420
FriOI GRGCYC 1 cut(s) 1258
Fsp4HI GCNGC 8 cut(s) 485, 654, 1293, 1417, 1873, 2410, 2413, 3420
FspBI CTAG 5 cut(s) 444, 1505, 1554, 2358, 2978
GlaI GCGC 1 cut(s) 1219
GluI GCNGC 8 cut(s) 485, 654, 1293, 1417, 1873, 2410, 2413, 3420
GsaI CCCAGC 1 cut(s) 875
GsuI CTGGAG 4 cut(s) 1763, 2218, 2768, 2809
HaeIII GGCC 6 cut(s) 707, 1256, 1541, 1644, 1687, 3047
HapII CCGG 5 cut(s) 44, 1221, 1229, 2451, 2939
HgaI GACGC 4 cut(s) 140, 1299, 1577, 3377
HhaI GCGC 1 cut(s) 1220
Hin1I GRCGYC 1 cut(s) 3388
Hin6I GCGC 1 cut(s) 1218
HinP1I GCGC 1 cut(s) 1218
HincII GTYRAC 1 cut(s) 2524
HindII GTYRAC 1 cut(s) 2524
HindIII AAGCTT 3 cut(s) 1926, 1988, 3119
HpaII CCGG 5 cut(s) 44, 1221, 1229, 2451, 2939
HphI GGTGA 5 cut(s) 769, 1546, 1669, 1744, 2777
Hpy166II GTNNAC 5 cut(s) 724, 1226, 1735, 2389, 2524
Hpy8I GTNNAC 5 cut(s) 724, 1226, 1735, 2389, 2524
Hpy99I CGWCG 1 cut(s) 1302
HpyCH4III ACNGT 5 cut(s) 2172, 2556, 2841, 3127, 3196
HpyCH4IV ACGT 3 cut(s) 2116, 3016, 3220
HpyF10VI GCNNNNNNNGC 1 cut(s) 1241
HpySE526I ACGT 3 cut(s) 2116, 3016, 3220
Hsp92I GRCGYC 1 cut(s) 3388
HspAI GCGC 1 cut(s) 1218
LguI GCTCTTC 1 cut(s) 1079
LmnI GCTCC 3 cut(s) 91, 770, 1134
Lsp1109I GCAGC 7 cut(s) 496, 665, 1279, 1403, 2399, 2421, 3431
MaeI CTAG 5 cut(s) 444, 1505, 1554, 2358, 2978
MaeII ACGT 3 cut(s) 2116, 3016, 3220
MaeIII GTNAC 4 cut(s) 2204, 2579, 2610, 2769
MfeI CAATTG 3 cut(s) 1446, 2048, 3110
MflI RGATCY 7 cut(s) 394, 908, 948, 1864, 2465, 2780, 2974
MhlI GDGCHC 2 cut(s) 775, 1258
MlsI TGGCCA 2 cut(s) 1687, 3047
MluNI TGGCCA 2 cut(s) 1687, 3047
MlyI GAGTC 4 cut(s) 729, 2482, 2800, 3025
MmeI TCCRAC 3 cut(s) 6, 721, 3126
Mox20I TGGCCA 2 cut(s) 1687, 3047
Mph1103I ATGCAT 2 cut(s) 1358, 1599
MscI TGGCCA 2 cut(s) 1687, 3047
MslI CAYNNNNRTG 1 cut(s) 2588
Msp20I TGGCCA 2 cut(s) 1687, 3047
MspA1I CMGCKG 3 cut(s) 1648, 2412, 2777
MspI CCGG 5 cut(s) 44, 1221, 1229, 2451, 2939
MspR9I CCNGG 2 cut(s) 2747, 3068
MunI CAATTG 3 cut(s) 1446, 2048, 3110
Mva1269I GAATGC 4 cut(s) 691, 1220, 2319, 3451
MvaI CCWGG 2 cut(s) 2747, 3068
MvnI CGCG 1 cut(s) 3396
MwoI GCNNNNNNNGC 1 cut(s) 1241
NcoI CCATGG 1 cut(s) 2602
NdeI CATATG 1 cut(s) 2996
NlaIV GGNNCC 5 cut(s) 396, 950, 1256, 2503, 3365
NsiI ATGCAT 2 cut(s) 1358, 1599
NspI RCATGY 2 cut(s) 2179, 2685
PciI ACATGT 1 cut(s) 2681
PciSI GCTCTTC 1 cut(s) 1079
PctI GAATGC 4 cut(s) 691, 1220, 2319, 3451
PfeI GAWTC 9 cut(s) 186, 263, 581, 1391, 1493, 1550, 1719, 1996, 2229
PflMI CCANNNNNTGG 1 cut(s) 1762
PinAI ACCGGT 2 cut(s) 43, 1228
PkrI GCNGC 8 cut(s) 486, 655, 1294, 1418, 1874, 2411, 2414, 3421
Ple19I CGATCG 1 cut(s) 166
PleI GAGTC 4 cut(s) 728, 2481, 2799, 3025
PmaCI CACGTG 1 cut(s) 3017
PmlI CACGTG 1 cut(s) 3017
PpsI GAGTC 4 cut(s) 728, 2481, 2799, 3025
Ppu21I YACGTR 1 cut(s) 3017
PscI ACATGT 1 cut(s) 2681
PsiI TTATAA 1 cut(s) 546
Psp6I CCWGG 2 cut(s) 2745, 3066
PspCI CACGTG 1 cut(s) 3017
PspFI CCCAGC 1 cut(s) 871
PspGI CCWGG 2 cut(s) 2745, 3066
PspN4I GGNNCC 5 cut(s) 396, 950, 1256, 2503, 3365
PspOMI GGGCCC 1 cut(s) 1254
PspPI GGNCC 4 cut(s) 1254, 1255, 1540, 3005
PstI CTGCAG 3 cut(s) 174, 2570, 3303
PsuI RGATCY 7 cut(s) 394, 908, 948, 1864, 2465, 2780, 2974
PvuI CGATCG 1 cut(s) 166
PvuII CAGCTG 2 cut(s) 2412, 2777
RsaI GTAC 7 cut(s) 408, 2174, 2185, 2485, 2838, 3245, 3437
RsaNI GTAC 7 cut(s) 407, 2173, 2184, 2484, 2837, 3244, 3436
RseI CAYNNNNRTG 1 cut(s) 2588
SapI GCTCTTC 1 cut(s) 1079
SatI GCNGC 8 cut(s) 485, 654, 1293, 1417, 1873, 2410, 2413, 3420
Sau96I GGNCC 4 cut(s) 1254, 1255, 1540, 3005
ScaI AGTACT 1 cut(s) 2485
SchI GAGTC 4 cut(s) 729, 2482, 2800, 3025
ScrFI CCNGG 2 cut(s) 2747, 3068
SduI GDGCHC 2 cut(s) 775, 1258
SfcI CTRYAG 6 cut(s) 170, 724, 2552, 2566, 3126, 3299
SinI GGWCC 1 cut(s) 3005
SmiMI CAYNNNNRTG 1 cut(s) 2588
SmlI CTYRAG 3 cut(s) 614, 1991, 2470
SmoI CTYRAG 3 cut(s) 614, 1991, 2470
SsiI CCGC 3 cut(s) 1648, 1873, 1895
SspI AATATT 1 cut(s) 2281
SspMI CTAG 5 cut(s) 444, 1505, 1554, 2358, 2978
StyD4I CCNGG 2 cut(s) 2745, 3066
StyI CCWWGG 1 cut(s) 2602
TaaI ACNGT 5 cut(s) 2172, 2556, 2841, 3127, 3196
TaiI ACGT 3 cut(s) 2119, 3019, 3223
TaqI TCGA 5 cut(s) 166, 1064, 1261, 1297, 3159
TatI WGTACW 6 cut(s) 2172, 2183, 2483, 2836, 3243, 3435
TauI GCSGC 1 cut(s) 1875
TfiI GAWTC 9 cut(s) 186, 263, 581, 1391, 1493, 1550, 1719, 1996, 2229
TscAI CASTG 5 cut(s) 494, 543, 1882, 2083, 3104
TseI GCWGC 7 cut(s) 484, 653, 1292, 1416, 2409, 2412, 3419
TspGWI ACGGA 2 cut(s) 1293, 2110
TspRI CASTG 5 cut(s) 494, 543, 1882, 2083, 3104
Van91I CCANNNNNTGG 1 cut(s) 1762
VpaK11BI GGWCC 1 cut(s) 3005
XapI RAATTY 9 cut(s) 369, 517, 647, 846, 1002, 1098, 1740, 2027, 2084
XbaI TCTAGA 1 cut(s) 2977
XceI RCATGY 2 cut(s) 2179, 2685
XcmI CCANNNNNNNNNTGG 1 cut(s) 316
XmiI GTMKAC 2 cut(s) 723, 1225
XspI CTAG 5 cut(s) 444, 1505, 1554, 2358, 2978
ZrmI AGTACT 1 cut(s) 2485
Zsp2I ATGCAT 2 cut(s) 1358, 1599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.