RchiOBHm_Chr5g0078301

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000085
Physical Location & Seq
Reverse (-)
84202375 .. 84207306
4932 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35283

Sequence Viewer

Length: 3219 bp
ATGGCCTCCAGCTCCCAAATAGCTTGTGCATCTTCTCATTCATCAGCTCCTTGCTGGACGTATGATGTGTTTTTGAGTTTCAGGGGTGTAGACACTCGCAAGGGGATTACAGTGGACATACACGATCGACTTAATCGGAGAGGAATTAAAACATTCATGGATGACCAAGATCTTGAAGTAGGGGATGCTATTTCTCCCACTCTCTTAGCAGCAATCAAAGAATCAAGGTTTGCAATCGTTGTTCTCTCGCAAAATTATGCTTCTTCTGCTTGGTGTTTGGAGGAACTTAGAGAAATTTGTCTATTAATGGAAGACAACAGAATTTTGCCACTTTTTTATCATGTTGATCCTACTGATGTTCGATATCAGAAGAGGAGTTTCGAAGAAGCTTTTTCTAAGCATGAAACCTCCTGGCGACATGAATCAGAGAAAGTGAAGCAATGGAAAGCTGCTTTAAACAAAGTGGCCGGTTTTTCTGGGTGGAATACAAATGATTATAAGACTCACAAAGAACTTGTTGATGTGATTGTGGAATTTCTCTGCAGTAAAGTAGTACCTGACGCAATTGAGTCCACAGGAGATTTTGATGCATTTGAAGCAACAAGACAAGCCATGGATGAAGTTATGAAGGCCTTTAAAGATGACGAGATCACTGCCATAGGTGTCTACGGCATGGGAGGCGTCGGCAAGACAACCATGGTAAAACATGTCGGTGCACATGGCCGAAAAAGTGGGATTTTTGATTATGTGATTATGGGTGTCGTCTCACAACGCCTTGACTACAGAAAAATTCAAGGCACATTGGCAGATCTGTTGGGCGTCAAATTGGAGGAGGAGACAGAAATTGGAAGAGCAGTTAGATTGAGAAATGAGATAATGAGAAGAACAAAGATCCTTATAATCCTGGACGACATTTGGGAGAGAATGGACTTGTCAGACATAGGAATACCTAGCTACAAGGAACTTCGAAAGTGCAAGTCCAAAGTGCTACTCACCACAAGGATAAGGAATGTCTGTCATGCCATGAAGTGCCAAGAAAAGATCAACCTCAATATCTTGTCAAAAGAAGATTCTTGGACCTTGTTTGTGAGAAATGCAGGAAGGTCTTTTGAATCCACCAATTTCGATGAGGTAGGGAGGAAGGTAGCTAGAGAATGTTGTGGTCTACCGATTGCATTGATAGCAGTTGCGAGGGCACTTGGAGATAAAGACTTGGTGGAATGGAAAAGAGCAGCTCAACGACTAGAGAAGTCGCAAGCTGCCAACCCTGACGATTACGGAGAAGCATCCAATTGTATAAAATTAAGCTATGATTACTTGAAAGATGAGGACTACAAATCATACTTCTTGCTCTGTTGCTTATTCCCAGAAGACGATGAAGTCAAAATTGAACACTTGTTCAGGTATGCTATTGGGAAAGGATTGTTTCGAGATGCCGACACACTCGAAGAAGCCAGAGAGAGAGCTGATTCCGTGGCCACGCACCTTAAACATTCTTGCTTGCTTTTGGATAGTGATACGGATGGATGTGTAAGGATGCATGATGTTGTCCGGGATACAGCCATTCAAATTGCGCAATCTGAAGATGGGTTTTTGGTGAAAGCTGGCTGTCGTTTAGTGGATTGGCCACGTCAATTACATGAAGACTACTCTGCAATCTCACTAATGGAGAATGATATTCAAAAGCTACCCGAAAAGTTGGTATGTCCAAAACTCCAGATTTTGTCACTGAAAAATAATATCTGGATAGAAAAGATACCAGAAACTTTCTTTCAAGGTCCGAATGAGTTAACAGTATTAGATCTTACCAGCACTAGTATTGCATTACTACCCGAATCATTCAGTCTGCTAACCAACCTCCAAGCTCTGTATTTAGATGGCTGTCAAGTCCTAACAATAAATAAAATTTCCATACTAGGAAGACTTAAAAAGCTTGAGATTCTTAGTATGAGAAACTGTTATTTGAAAGAGTTGTCAAGAGAAATAGGAAATTTGACCAATTTAAGGATGTTGGATATCACTGGTGGTGATATGAATACAATTTCATCAAAAGTGATTTCAAAGTTGCATAAGTTAGAAGAATTGTACATGCAATGTGGATTTCACGATTGGGGGAGTAAAGTCAGGGGAGAAGGAGAAGAAACCAATGCTAGCTTTGATGAGGTAACTAGCTTGTCAAATCTAAGAGTTTTGGAGGTTTGTATCTCTGATGCAGAATGTATCCCTAAAGATGTCAAGTTCAATCCGAATTGGGTCAAGTTTGATATATGTATTGGCAGAGACCGCTACAAGGCATCGTATGCATTTAGTCATAATTCAATATCCTTAATGCTTGACACCACTAGTAGCTTCCCAGACTGGTTTCTCAACGTGGTGATGAACAAAACACAAAGGATAGTGTGGGAAGGGTGCCAAAGGTTGATCAAAATTTTTGTGGATTATGAACATGGGAGGTTACAAGAATTGAAACATCTCTCTGTATATGGTGACGATTGCTATGAGCTGATGAATGCATTAACATGGGTTCCAAAAAAACCTGTGTTCGAGAACTTGGAAAAGTTGCATTTGAGTGACGTAGATTGCCATGAGTTATGTGTTGGTGACTTACCAGTTGGATCTCTATTCAATTTGCAGATATTGAAGGTACACTCTTGTCTCAACTGGGGGAATGTACTTTTACCATCAGTACTGTTACCAAATCTGGAAGAACTAAATTGTAGTTTCATCAACATTGAATATGTGTTTGGCTGTGAAGCATTATCTGAGCCAAAACAATCAAAATTGAGAAAGATGGAATTGTCTAATCTATATACAGTACGAAGCATATGTGATGGACCAGCTCCACCTGCAATGTTACAGACTCTTCAAAGTTTATCCATTAAGGAGTGCTCCTTGCAGGGAAGTCTCTTCACCTCTGATGTTGCTCAGTGTCTTTCTCAGTTGAATTACTTAAATTTAGAGCATTGCTCCTCTTTGGAGAGAATTGTTGAAGCAAGCAACAAGAAGATCATTATTCCGAAATTGAAGGAATTAAGTTTGACGGGACTGGACATGTTGTACTATGAAAGTGCTACTTTTGATATTGAGTGTCCTTCATTGGAAGAATTGTCTATTTGGAACTGCCCCAAGTTTTCAATCTCTCCTTCTGACTTCCACAGCAGCAAACAAGTCCAACTCAACAGATTGTTCGAGAGCATAACAAGAAGAAGAAGAAGATGA

Protein Analysis

1072

Amino Acids

122.01

Weight (kDa)

5.68

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 21 - 183 4.5e-44 TIR domain
TIR_2 PF13676 23 - 117 1.5e-15 TIR domain
NB-ARC PF00931 203 - 366 6e-32 NB-ARC domain
LRR_8 PF13855 570 - 629 1.4e-10 Leucine rich repeat
LRR_14 PF23598 572 - 705 5e-12 Leucine-rich repeat region
LRR_RPS2 PF23247 850 - 989 1.8e-15 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 928 - 1045 8.3e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 498, 899
AarI CACCTGC 1 cut(s) 2854
AasI GACNNNNNNGTC 1 cut(s) 1379
Acc16I TGCGCA 1 cut(s) 1575
Acc36I ACCTGC 1 cut(s) 2854
AccB1I GGYRCC 1 cut(s) 2409
AccI GTMKAC 3 cut(s) 90, 666, 1165
AciI CCGC 1 cut(s) 2284
AclWI GGATC 3 cut(s) 341, 886, 2623
AcoI YGGCCR 4 cut(s) 465, 721, 1476, 1625
AcsI RAATTY 8 cut(s) 294, 321, 533, 789, 1905, 1990, 2427, 2953
AcuI CTGAAG 1 cut(s) 1602
AcyI GRCGYC 2 cut(s) 681, 819
AfaI GTAC 7 cut(s) 555, 2087, 2646, 2673, 2688, 2817, 3059
AfiI CCNNNNNNNGG 2 cut(s) 2004, 2290
AflIII ACRYGT 2 cut(s) 706, 3051
AhlI ACTAGT 2 cut(s) 1814, 2342
AjiI CACGTC 1 cut(s) 1631
AjnI CCWGG 2 cut(s) 410, 903
AjuI GAANNNNNNNTTGG 2 cut(s) 2727, 2759
Alw21I GWGCWC 2 cut(s) 718, 2891
Alw26I GTCTC 5 cut(s) 769, 830, 2274, 2660, 2909
Alw44I GTGCAC 1 cut(s) 714
AlwI GGATC 3 cut(s) 341, 886, 2623
AoxI GGCC 6 cut(s) 3, 465, 630, 721, 1476, 1625
ApaLI GTGCAC 1 cut(s) 714
ApeKI GCWGC 5 cut(s) 209, 449, 1232, 1259, 3159
ApoI RAATTY 8 cut(s) 294, 321, 533, 789, 1905, 1990, 2427, 2953
AseI ATTAAT 1 cut(s) 305
AspLEI GCGC 1 cut(s) 1576
AspS9I GGNCC 3 cut(s) 1077, 1778, 2834
AsuC2I CCSGG 1 cut(s) 1553
AsuHPI GGTGA 7 cut(s) 985, 1609, 2039, 2386, 2498, 2612, 2902
AsuII TTCGAA 2 cut(s) 381, 967
AsuNHI GCTAGC 1 cut(s) 2150
AvaII GGWCC 3 cut(s) 1077, 1778, 2834
BaeGI GKGCMC 2 cut(s) 718, 1198
BalI TGGCCA 2 cut(s) 1478, 1627
BanI GGYRCC 1 cut(s) 2409
BbsI GAAGAC 4 cut(s) 318, 1377, 1650, 1927
Bbv12I GWGCWC 2 cut(s) 718, 2891
BbvI GCAGC 5 cut(s) 221, 436, 1244, 1246, 3171
BccI CCATC 6 cut(s) 1517, 1580, 1871, 2689, 2785, 2825
BceAI ACGGC 1 cut(s) 685
BcgI CGANNNNNNTGC 2 cut(s) 635, 669
BciT130I CCWGG 2 cut(s) 412, 905
BciVI GTATCC 2 cut(s) 1549, 2231
BclI TGATCA 1 cut(s) 2421
BcnI CCSGG 1 cut(s) 1553
BcoDI GTCTC 5 cut(s) 769, 830, 2274, 2660, 2909
BcuI ACTAGT 2 cut(s) 1814, 2342
BfaI CTAG 8 cut(s) 951, 1149, 1244, 1815, 1916, 2151, 2169, 2343
BfmI CTRYAG 2 cut(s) 541, 781
BfuAI ACCTGC 1 cut(s) 2854
BfuI GTATCC 2 cut(s) 1549, 2231
BglII AGATCT 3 cut(s) 169, 808, 1801
BisI GCNGC 5 cut(s) 210, 450, 1233, 1260, 3160
BlsI GCNGC 5 cut(s) 211, 451, 1234, 1261, 3161
BmcAI AGTACT 1 cut(s) 2688
Bme1390I CCNGG 3 cut(s) 412, 905, 1553
Bme18I GGWCC 3 cut(s) 1077, 1778, 2834
BmgBI CACGTC 1 cut(s) 1631
BmgT120I GGNCC 3 cut(s) 1077, 1778, 2834
BmiI GGNNCC 2 cut(s) 2411, 2526
BmrFI CCNGG 3 cut(s) 412, 905, 1553
BmrI ACTGGG 1 cut(s) 2671
BmsI GCATC 8 cut(s) 38, 175, 577, 1295, 1423, 1527, 2200, 2303
BmtI GCTAGC 1 cut(s) 2154
BmuI ACTGGG 1 cut(s) 2671
BpiI GAAGAC 4 cut(s) 318, 1377, 1650, 1927
BplI GAGNNNNNCTC 2 cut(s) 2951, 2983
BpmI CTGGAG 1 cut(s) 1700
Bpu14I TTCGAA 2 cut(s) 381, 967
BpuEI CTTGAG 1 cut(s) 1955
BpuMI CCSGG 1 cut(s) 1553
BsaHI GRCGYC 2 cut(s) 681, 819
BsaI GGTCTC 1 cut(s) 2274
BsaJI CCNNGG 3 cut(s) 612, 696, 1473
Bsc4I CCNNNNNNNGG 2 cut(s) 2004, 2290
Bse118I RCCGGY 1 cut(s) 467
Bse1I ACTGG 5 cut(s) 2026, 2363, 2609, 2666, 3051
Bse3DI GCAATG 4 cut(s) 446, 2099, 2856, 2962
BseBI CCWGG 2 cut(s) 412, 905
BseDI CCNNGG 3 cut(s) 612, 696, 1473
BseGI GGATG 8 cut(s) 166, 190, 622, 1286, 1528, 1532, 1542, 2013
BseLI CCNNNNNNNGG 2 cut(s) 2004, 2290
BseMI GCAATG 4 cut(s) 446, 2099, 2856, 2962
BseMII CTCAG 3 cut(s) 2754, 2939, 2951
BseNI ACTGG 5 cut(s) 2026, 2363, 2609, 2666, 3051
BseRI GAGGAG 4 cut(s) 388, 845, 848, 2959
BseSI GKGCMC 2 cut(s) 718, 1198
BseXI GCAGC 5 cut(s) 221, 436, 1244, 1246, 3171
Bsh1285I CGRYCG 1 cut(s) 127
BshFI GGCC 6 cut(s) 5, 467, 632, 723, 1478, 1627
BshNI GGYRCC 1 cut(s) 2409
BsiEI CGRYCG 1 cut(s) 127
BsiHKAI GWGCWC 2 cut(s) 718, 2891
BsiSI CCGG 2 cut(s) 468, 1552
BslFI GGGAC 1 cut(s) 3057
BslI CCNNNNNNNGG 2 cut(s) 2004, 2290
BsmAI GTCTC 5 cut(s) 769, 830, 2274, 2660, 2909
BsmBI CGTCTC 1 cut(s) 769
BsmFI GGGAC 1 cut(s) 3057
BsmI GAATGC 1 cut(s) 2515
BsnI GGCC 6 cut(s) 5, 467, 632, 723, 1478, 1627
Bso31I GGTCTC 1 cut(s) 2274
Bsp119I TTCGAA 2 cut(s) 381, 967
Bsp1286I GDGCHC 3 cut(s) 718, 1198, 2891
Bsp1407I TGTACA 1 cut(s) 2085
Bsp19I CCATGG 2 cut(s) 612, 696
BspACI CCGC 1 cut(s) 2284
BspANI GGCC 6 cut(s) 5, 467, 632, 723, 1478, 1627
BspCNI CTCAG 3 cut(s) 2755, 2938, 2950
BspLI GGNNCC 2 cut(s) 2411, 2526
BspMAI CTGCAG 1 cut(s) 545
BspMI ACCTGC 1 cut(s) 2854
BspOI GCTAGC 1 cut(s) 2154
BspPI GGATC 3 cut(s) 341, 886, 2623
BspQI GCTCTTC 1 cut(s) 844
BspT104I TTCGAA 2 cut(s) 381, 967
BspT107I GGYRCC 1 cut(s) 2409
BspTNI GGTCTC 1 cut(s) 2274
BsrDI GCAATG 4 cut(s) 446, 2099, 2856, 2962
BsrFI RCCGGY 1 cut(s) 467
BsrGI TGTACA 1 cut(s) 2085
BsrI ACTGG 5 cut(s) 2026, 2363, 2609, 2666, 3051
BssAI RCCGGY 1 cut(s) 467
BssECI CCNNGG 3 cut(s) 612, 696, 1473
BssNI GRCGYC 2 cut(s) 681, 819
BssT1I CCWWGG 2 cut(s) 612, 696
Bst2UI CCWGG 2 cut(s) 412, 905
Bst4CI ACNGT 5 cut(s) 112, 1795, 1958, 2691, 2815
Bst6I CTCTTC 4 cut(s) 365, 844, 2868, 2912
BstACI GRCGYC 2 cut(s) 681, 819
BstAPI GCANNNNNTGC 1 cut(s) 2300
BstAUI TGTACA 1 cut(s) 2085
BstBI TTCGAA 2 cut(s) 381, 967
BstC8I GCNNGC 5 cut(s) 1257, 1502, 1606, 2152, 2995
BstDEI CTNAG 8 cut(s) 205, 287, 396, 1943, 2183, 2763, 2925, 2937
BstDSI CCRYGG 3 cut(s) 612, 696, 1473
BstF5I GGATG 8 cut(s) 166, 190, 622, 1286, 1528, 1532, 1542, 2013
BstHHI GCGC 1 cut(s) 1576
BstMAI GTCTC 5 cut(s) 769, 830, 2274, 2660, 2909
BstMCI CGRYCG 1 cut(s) 127
BstMWI GCNNNNNNNGC 7 cut(s) 266, 596, 678, 1181, 2283, 2300, 2846
BstNI CCWGG 2 cut(s) 412, 905
BstNSI RCATGY 3 cut(s) 710, 2092, 3055
BstSCI CCNGG 3 cut(s) 410, 903, 1551
BstSFI CTRYAG 2 cut(s) 541, 781
BstSLI GKGCMC 2 cut(s) 718, 1198
BstV1I GCAGC 5 cut(s) 221, 436, 1244, 1246, 3171
BstV2I GAAGAC 4 cut(s) 318, 1377, 1650, 1927
BstX2I RGATCY 5 cut(s) 169, 808, 891, 1801, 2615
BstYI RGATCY 5 cut(s) 169, 808, 891, 1801, 2615
BsuI GTATCC 2 cut(s) 1549, 2231
BsuRI GGCC 6 cut(s) 5, 467, 632, 723, 1478, 1627
BtgI CCRYGG 3 cut(s) 612, 696, 1473
BtrI CACGTC 1 cut(s) 1631
BtsCI GGATG 8 cut(s) 166, 190, 622, 1286, 1528, 1532, 1542, 2013
BtsI GCAGTG 1 cut(s) 651
BtsIMutI CAGTG 5 cut(s) 117, 651, 1727, 2019, 2933
BveI ACCTGC 1 cut(s) 2854
Cac8I GCNNGC 5 cut(s) 1257, 1502, 1606, 2152, 2995
CfoI GCGC 1 cut(s) 1576
Cfr10I RCCGGY 1 cut(s) 467
Cfr13I GGNCC 3 cut(s) 1077, 1778, 2834
CseI GACGC 3 cut(s) 569, 670, 808
Csp6I GTAC 7 cut(s) 554, 2086, 2645, 2672, 2687, 2816, 3058
CviQI GTAC 7 cut(s) 554, 2086, 2645, 2672, 2687, 2816, 3058
DdeI CTNAG 8 cut(s) 205, 287, 396, 1943, 2183, 2763, 2925, 2937
DraI TTTAAA 2 cut(s) 456, 637
DrdI GACNNNNNNGTC 1 cut(s) 1379
DseDI GACNNNNNNGTC 1 cut(s) 1379
EaeI YGGCCR 4 cut(s) 465, 721, 1476, 1625
Eam1104I CTCTTC 4 cut(s) 365, 844, 2868, 2912
EarI CTCTTC 4 cut(s) 365, 844, 2868, 2912
Eco130I CCWWGG 2 cut(s) 612, 696
Eco147I AGGCCT 1 cut(s) 632
Eco31I GGTCTC 1 cut(s) 2274
Eco32I GATATC 2 cut(s) 365, 2017
Eco47I GGWCC 3 cut(s) 1077, 1778, 2834
Eco57I CTGAAG 1 cut(s) 1602
EcoRII CCWGG 2 cut(s) 410, 903
EcoRV GATATC 2 cut(s) 365, 2017
EcoT14I CCWWGG 2 cut(s) 612, 696
EcoT22I ATGCAT 4 cut(s) 592, 1542, 2305, 2515
ErhI CCWWGG 2 cut(s) 612, 696
Esp3I CGTCTC 1 cut(s) 769
FalI AAGNNNNNCTT 2 cut(s) 3058, 3090
FaqI GGGAC 1 cut(s) 3057
FauNDI CATATG 1 cut(s) 2825
FbaI TGATCA 1 cut(s) 2421
FblI GTMKAC 3 cut(s) 90, 666, 1165
Fnu4HI GCNGC 5 cut(s) 210, 450, 1233, 1260, 3160
FokI GGATG 8 cut(s) 173, 197, 629, 1273, 1535, 1539, 1549, 2020
Fsp4HI GCNGC 5 cut(s) 210, 450, 1233, 1260, 3160
FspBI CTAG 8 cut(s) 951, 1149, 1244, 1815, 1916, 2151, 2169, 2343
FspI TGCGCA 1 cut(s) 1575
GlaI GCGC 1 cut(s) 1575
GluI GCNGC 5 cut(s) 210, 450, 1233, 1260, 3160
GsuI CTGGAG 1 cut(s) 1700
HaeIII GGCC 6 cut(s) 5, 467, 632, 723, 1478, 1627
HapII CCGG 2 cut(s) 468, 1552
HgaI GACGC 3 cut(s) 569, 670, 808
HhaI GCGC 1 cut(s) 1576
Hin1I GRCGYC 2 cut(s) 681, 819
Hin6I GCGC 1 cut(s) 1574
HinP1I GCGC 1 cut(s) 1574
HincII GTYRAC 1 cut(s) 1791
HindII GTYRAC 1 cut(s) 1791
HindIII AAGCTT 2 cut(s) 387, 1931
HpaI GTTAAC 1 cut(s) 1791
HpaII CCGG 2 cut(s) 468, 1552
HphI GGTGA 7 cut(s) 985, 1609, 2039, 2386, 2498, 2612, 2902
Hpy166II GTNNAC 8 cut(s) 91, 115, 573, 667, 716, 1166, 1791, 2647
Hpy188III TCNNGA 9 cut(s) 173, 1430, 1717, 1744, 1977, 2105, 2545, 2702, 3190
Hpy8I GTNNAC 8 cut(s) 91, 115, 573, 667, 716, 1166, 1791, 2647
Hpy99I CGWCG 1 cut(s) 686
HpyAV CCTTC 9 cut(s) 622, 1095, 1135, 2126, 2399, 2635, 3019, 3102, 3153
HpyCH4III ACNGT 5 cut(s) 112, 1795, 1958, 2691, 2815
HpyCH4IV ACGT 4 cut(s) 59, 1630, 2370, 2574
HpyF10VI GCNNNNNNNGC 7 cut(s) 266, 596, 678, 1181, 2283, 2300, 2846
HpyF3I CTNAG 8 cut(s) 205, 287, 396, 1943, 2183, 2763, 2925, 2937
HpySE526I ACGT 4 cut(s) 59, 1630, 2370, 2574
Hsp92I GRCGYC 2 cut(s) 681, 819
HspAI GCGC 1 cut(s) 1574
Ksp22I TGATCA 1 cut(s) 2421
KspAI GTTAAC 1 cut(s) 1791
LguI GCTCTTC 1 cut(s) 844
LmnI GCTCC 5 cut(s) 17, 52, 2845, 2894, 2972
Lsp1109I GCAGC 5 cut(s) 221, 436, 1244, 1246, 3171
LweI GCATC 8 cut(s) 38, 175, 577, 1295, 1423, 1527, 2200, 2303
MaeI CTAG 8 cut(s) 951, 1149, 1244, 1815, 1916, 2151, 2169, 2343
MaeII ACGT 4 cut(s) 59, 1630, 2370, 2574
MaeIII GTNAC 8 cut(s) 1725, 2164, 2454, 2486, 2570, 2600, 2691, 2853
MfeI CAATTG 2 cut(s) 564, 1291
MflI RGATCY 5 cut(s) 169, 808, 891, 1801, 2615
MhlI GDGCHC 3 cut(s) 718, 1198, 2891
MlsI TGGCCA 2 cut(s) 1478, 1627
MluNI TGGCCA 2 cut(s) 1478, 1627
MlyI GAGTC 3 cut(s) 496, 578, 2854
MmeI TCCRAC 3 cut(s) 1992, 2593, 3196
Mox20I TGGCCA 2 cut(s) 1478, 1627
Mph1103I ATGCAT 4 cut(s) 592, 1542, 2305, 2515
MscI TGGCCA 2 cut(s) 1478, 1627
MslI CAYNNNNRTG 3 cut(s) 711, 2518, 2568
Msp20I TGGCCA 2 cut(s) 1478, 1627
MspI CCGG 2 cut(s) 468, 1552
MspR9I CCNGG 3 cut(s) 412, 905, 1553
MunI CAATTG 2 cut(s) 564, 1291
Mva1269I GAATGC 1 cut(s) 2515
MvaI CCWGG 2 cut(s) 412, 905
MwoI GCNNNNNNNGC 7 cut(s) 266, 596, 678, 1181, 2283, 2300, 2846
NciI CCSGG 1 cut(s) 1553
NcoI CCATGG 2 cut(s) 612, 696
NdeI CATATG 1 cut(s) 2825
NheI GCTAGC 1 cut(s) 2150
NlaIV GGNNCC 2 cut(s) 2411, 2526
NmuCI GTSAC 4 cut(s) 1725, 2486, 2570, 2600
NsbI TGCGCA 1 cut(s) 1575
NsiI ATGCAT 4 cut(s) 592, 1542, 2305, 2515
NspI RCATGY 3 cut(s) 710, 2092, 3055
NspV TTCGAA 2 cut(s) 381, 967
PaqCI CACCTGC 1 cut(s) 2854
PceI AGGCCT 1 cut(s) 632
PciI ACATGT 2 cut(s) 706, 3051
PciSI GCTCTTC 1 cut(s) 844
PctI GAATGC 1 cut(s) 2515
PfeI GAWTC 7 cut(s) 221, 422, 1070, 1112, 1469, 1835, 1939
PfoI TCCNGGA 2 cut(s) 903, 1551
PkrI GCNGC 5 cut(s) 211, 451, 1234, 1261, 3161
Ple19I CGATCG 1 cut(s) 127
PleI GAGTC 3 cut(s) 496, 577, 2854
PpsI GAGTC 3 cut(s) 496, 577, 2854
PscI ACATGT 2 cut(s) 706, 3051
PshBI ATTAAT 1 cut(s) 305
PsiI TTATAA 2 cut(s) 498, 899
Psp6I CCWGG 2 cut(s) 410, 903
PspGI CCWGG 2 cut(s) 410, 903
PspN4I GGNNCC 2 cut(s) 2411, 2526
PspPI GGNCC 3 cut(s) 1077, 1778, 2834
PstI CTGCAG 1 cut(s) 545
PsuI RGATCY 5 cut(s) 169, 808, 891, 1801, 2615
PvuI CGATCG 1 cut(s) 127
RsaI GTAC 7 cut(s) 555, 2087, 2646, 2673, 2688, 2817, 3059
RsaNI GTAC 7 cut(s) 554, 2086, 2645, 2672, 2687, 2816, 3058
RseI CAYNNNNRTG 3 cut(s) 711, 2518, 2568
SapI GCTCTTC 1 cut(s) 844
SatI GCNGC 5 cut(s) 210, 450, 1233, 1260, 3160
Sau96I GGNCC 3 cut(s) 1077, 1778, 2834
ScaI AGTACT 1 cut(s) 2688
SchI GAGTC 3 cut(s) 496, 578, 2854
ScrFI CCNGG 3 cut(s) 412, 905, 1553
SduI GDGCHC 3 cut(s) 718, 1198, 2891
SfaNI GCATC 8 cut(s) 38, 175, 577, 1295, 1423, 1527, 2200, 2303
SfcI CTRYAG 2 cut(s) 541, 781
SfuI TTCGAA 2 cut(s) 381, 967
SinI GGWCC 3 cut(s) 1077, 1778, 2834
SmiMI CAYNNNNRTG 3 cut(s) 711, 2518, 2568
SmlI CTYRAG 1 cut(s) 1934
SmoI CTYRAG 1 cut(s) 1934
SpeI ACTAGT 2 cut(s) 1814, 2342
SseBI AGGCCT 1 cut(s) 632
SsiI CCGC 1 cut(s) 2284
SspMI CTAG 8 cut(s) 951, 1149, 1244, 1815, 1916, 2151, 2169, 2343
StuI AGGCCT 1 cut(s) 632
StyD4I CCNGG 3 cut(s) 410, 903, 1551
StyI CCWWGG 2 cut(s) 612, 696
TaaI ACNGT 5 cut(s) 112, 1795, 1958, 2691, 2815
TaiI ACGT 4 cut(s) 62, 1633, 2373, 2577
TaqI TCGA 9 cut(s) 127, 361, 381, 967, 1125, 1429, 1446, 2544, 3189
TatI WGTACW 4 cut(s) 2085, 2671, 2686, 3057
TfiI GAWTC 7 cut(s) 221, 422, 1070, 1112, 1469, 1835, 1939
TscAI CASTG 5 cut(s) 117, 658, 1734, 2026, 2933
TseFI GTSAC 4 cut(s) 1725, 2486, 2570, 2600
TseI GCWGC 5 cut(s) 209, 449, 1232, 1259, 3159
Tsp45I GTSAC 4 cut(s) 1725, 2486, 2570, 2600
TspGWI ACGGA 3 cut(s) 1293, 1462, 1535
TspRI CASTG 5 cut(s) 117, 658, 1734, 2026, 2933
VneI GTGCAC 1 cut(s) 714
VpaK11BI GGWCC 3 cut(s) 1077, 1778, 2834
VspI ATTAAT 1 cut(s) 305
XapI RAATTY 8 cut(s) 294, 321, 533, 789, 1905, 1990, 2427, 2953
XceI RCATGY 3 cut(s) 710, 2092, 3055
XmiI GTMKAC 3 cut(s) 90, 666, 1165
XspI CTAG 8 cut(s) 951, 1149, 1244, 1815, 1916, 2151, 2169, 2343
ZrmI AGTACT 1 cut(s) 2688
Zsp2I ATGCAT 4 cut(s) 592, 1542, 2305, 2515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.