Rw0G022800

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig01057
OG0000085
Physical Location & Seq
Reverse (-)
31132 .. 34936
3805 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G022800.1

Sequence Viewer

Length: 3642 bp
GCGATGAGAATGATTATTGTGATTAGTGTGGTAATGGTGCAATGGTTCGGTGTCATAGCCAGTCTTAAGTGGATATGGAAGAATTGGTTCATTATTGTGGTAATTGTGACATGGAACGTAATAGCCAGTGTTGGGTGGATATGGAACAGTAGGTTCAGTTACATGGTTGAAACCCCAGAGCCAATATCAGAAGATAATCAAGAACTGGTCGACGTCGTTGAAACCCCAGAGCCAATATCAGAGGATAATCAAGAACTGGTCGACGTCGTTGAAACCCCAGAGCCAATATCAGAGGATAATCAAGAGCCGGTTGACGTCGATGTCTCTGCATCTCTTCCTTCATCATTAGCTGAATCATCAGCTCCTAGGTGGAAGCATGATGTGTTTTTGAGCTTCAGGGGTGCAGACACTCGTAAGGGTATTACCTCCTTTTTATACGATCAACTGCAAAACAGAGGAATCAAAACATTCATGGATGACCAGGACCTTCAAGTAGGGGATGTTATTTCTCCCACTCTCCTAACGGCAATTAAAGAATCAAGGTTTGCAGTTATTGTTCTCTCTCAAAACTATGCCTCTTCCACTTGGTGTTTGGAGGAACTTAGAAACATTTGTGAGTGCATGGAAGAAGACAAAAATAGAATTCTCCCACTTTTTTATTATGTGGATCCTACTGATGTACGACATCAGAAGAGGAGTTTCGGAGATGCTTTCTCTAAGCATGAAAATTTTGGGCGACACAGATCAGAGAAGCTGCAGCAGTGGAGATATGCTTTAACAAAAGTGGCAAATTTCTCCGGGTGGCATACACTGAATTATAAAACTGAAAGAGAACTTTTGGATGCCATTGAGGAATATGTGTGCAATAAATTACGACCTACTGAACTTGAGCTTAAAATGTCCATTGGAGGTTTTGAAGCATTTGAAGCAACAAAACAAGCCATTGATAAGGTCATGAATGCGATAAAAGACGATGAGGTTACTACCATTGGGGTCTATGGAATGGGGGGCGTTGGAAAGACGACAATGGTGAAATATGTTGGAGCACAAGCCCAAGAAAGTAGGCTTTTTAATAAAGTGATTATGGCTGTTGTATCCCAAAACCCCGACTTGATGAAAATTCAAGAGACATTTGCAGAACTGCTGGGCTTTAAATTGGGGGAGAAGACAGAAATTGGAAGAGCCAGCAAATTGACGGAGAATATAATGAGAGGAACTGGGATCCTCATAATCTTGGATGACATTTGGAAGAGAATAGACTTTTCAAGGATTGGAATTCCGAGCCACAATGAACTTCGAAGATGCAATTCCAAAGTTCTACTGACCACCAGAAAATTGAGCGTTTGCCGCTCCATGGATTGCCATGCAAACATACATCTCAATATCTTATCAGAAGAAGATTCTTGGAGCTTATTTTTGAAGGAAGCAAGGAAGTCTTTTGACAAATCATCCAATTTCTATGATGTAGCACGTAAGGTGGCTAGAGAATGCGCTGGTCTACCAATTGCTTTGATAGCAGTTTCGAGGGCCCTTCGAGATGAAGGTTTGGACGGATGGAAAGAAGCTGCTCGACGACTAAAAGCATCTCAACCTGCCAACCCTGAAGATGAGAGAGATGTGTTTGCATGCATAAAGTTAAGCTATGATTACTTGAAATCTGATTATTCCAAATCATGCTTCTTGCTTTGCTGCCTGTTCCCAGAAGATTATGAAATCCCCATTGAATGCTTGCTCATGTATGGAATTGGGAAAGGAATGTTTCAAGATTCCAACATGCTAGAAGCCCGAGACACTACATATTCAGTGGTGAAGGCCCTTAAAGATTCTAGCTTGCTTTTGGACGCTAGAGATAACGGATGTGTAAGGATACATGATGTCATTCGGGATATGGCAATATTGATTTCATTATCTGAAGACGGCCAGCGGTTCTTGGTGAAAGTTGGCTATGAATTGAAAAATTGGCCAAAGATTGATGAACATAAAGGCTACTCTGCAATCTCACTAATGAAGAACAAAATTTGGAAGCTACCGGAAGAGTTGGTATGTCCAAATCTCCAGATTTTATTACTGCAAGACAATTCTTCATTAAATGAGATCCCAAAATCTTTTTTCCAAAGTCAGAATGAATTAAGGGTCTTGGATCTTAGCGACACTGGTATTTCATTACTACCTCAATCAATCAGTTTCCTAACCAACCTTCAAGCTTTGTATTTAGATTATTGCGAGAACATAATTGACATTTCTGTAATCGGAAAACTTCACAAGCTTGAGATTCTTAGTATGAGAAGACCTGCTCAGAAAGAATTGTCACGAGAAATAGGGCAGTTGACCAATCTAAAGATGCTGGATGTCAGTGCTGGTAGATTCTCGGGTGGACGTATTTGCACAATTCCGTCTAAAGTGATATCAAAGTTGCATAAATTAGAAGAACTGTACATGCAATATTGTGGATTTTGGGACTGGGGGAGTAAAATTGATGGAGAAAGAGAAGAAACTAATATTGGCTTTGATGAATTAGTTGGTTTATCATATTTAAGCGTTTTGAAAGTTTGCATATCAGATGCAAATTGCATCCCTAGAAGTGTTGAGGTCGAACCGAATTGGGTTTACTTTGATATTAGTATTTGCAGCGGCAGTGATAGAGAGAGAGCCATTATGAATCTTCCATCCGGTCATAATTGTAGATACTTGAGTCTTGATAGTACTGACACAACCATAGGAACCTTTCCGGACTGGTTTGTCAACGCTGTGGTAAAGAATACTGAGAGGCTACGTTATAAAGACTGCAAAGGGTTAAGTAACATTCTTGTGGAATATGACCATGGGAGGTTACATGAACTGAAAGTTCTCTATGTAAAAGTTCGCTGTGAGAACTTGAAAGAATTGATGAATGCAATAACATATGTTCCATATATGCCTGTGTTTGAGAACTTGGAAGAGTTGTATCTGCAATACCTGGATGACCTGAAGCAGTTATGTGTTGGAGAGTTACCACCTGGATCTCTCTGCAGTCTGAAATTATTGATGGTCCGTGAGTGTCCTAACTTGGGAAATATACTATTGCCATCAAAATTGTGGCAGAAACTACGAAATTTGGAAATACTAATCTGTATGAGAAGTAAAGTGGAACATGTGTTTGAATGTGAAGGATTTGAGCCAGATCAAACAAATCTGAGAAGGATGAAGTTATATGATCTAGATGTACTAAGAAGCATATGTAATGGTCCTGCTCCACGTGGAATGTTCCAGACTCTAAAGAAATTGGTCATTTGCACTTGCAACATCCAGGGAAGTCTCTTCACATTTGATGTAGCTCAGTTTCTTTTTCAATTGGAAACCCTTACAATATCCAGATGCCCTGTCTTGGAAAGAGTAATCGAAGCAAGGAGAGAAGCATTGAACAACAAGAAGACCGTTCTTCCAAAATTGAAGAACTTATGTTTGCTACATCTTCCAATGTTGTACATGGGAAGTGCTACTATTGATTTTGAGTGTCCTTCATTGGAAAACTTGCGTCTAAAGGGGTGCCCCTACTTGTCATTTCCATCCTCTGCTTCTGACTACTTCCACAGCAGGAACCAAGTCCTTTCCAATGATCCTCTCTGCTTATATAATTGGAAGGCTAGTTTGAATTCCTGCTAG

Protein Analysis

1213

Amino Acids

138.6

Weight (kDa)

5.62

Isoelectric Point (pI)

48.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 125 - 295 7e-51 TIR domain
TIR_2 PF13676 128 - 238 2.7e-17 TIR domain
NB-ARC PF00931 312 - 477 8.7e-30 NB-ARC domain
LRR_8 PF13855 683 - 741 8.4e-09 Leucine rich repeat
LRR_14 PF23598 689 - 875 2e-14 Leucine-rich repeat region
LRR_RPS2 PF23247 978 - 1119 2.8e-20 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1081 - 1180 4.5e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 819, 2778
AasI GACNNNNNNGTC 2 cut(s) 320, 2738
AatII GACGTC 3 cut(s) 216, 267, 318
Acc36I ACCTGC 2 cut(s) 1600, 2299
AccB1I GGYRCC 1 cut(s) 3525
AccBSI CCGCTC 1 cut(s) 1350
AccI GTMKAC 3 cut(s) 210, 261, 1498
AccIII TCCGGA 1 cut(s) 2728
AciI CCGC 3 cut(s) 1348, 1924, 2631
AclWI GGATC 8 cut(s) 662, 675, 1216, 1229, 2089, 2148, 3007, 3590
AcoI YGGCCR 2 cut(s) 1918, 1961
AcsI RAATTY 8 cut(s) 642, 727, 790, 1119, 1275, 2016, 3091, 3631
AcuI CTGAAG 4 cut(s) 379, 1623, 1932, 2987
AcvI CACGTG 1 cut(s) 3236
AcyI GRCGYC 3 cut(s) 213, 264, 315
AdeI CACNNNGTG 1 cut(s) 588
AfaI GTAC 5 cut(s) 681, 2435, 2704, 3204, 3464
AfiI CCNNNNNNNGG 4 cut(s) 132, 1354, 3047, 3364
AflII CTTAAG 1 cut(s) 65
AflIII ACRYGT 1 cut(s) 3130
AjnI CCWGG 4 cut(s) 480, 2955, 2995, 3285
AjuI GAANNNNNNNTTGG 4 cut(s) 1661, 1693, 2484, 2516
Alw21I GWGCWC 1 cut(s) 1048
Alw26I GTCTC 4 cut(s) 328, 1121, 1782, 3299
AlwI GGATC 8 cut(s) 662, 675, 1216, 1229, 2089, 2148, 3007, 3590
Ama87I CYCGRG 2 cut(s) 1785, 2368
Aor13HI TCCGGA 1 cut(s) 2728
AoxI GGCC 4 cut(s) 1527, 1812, 1918, 1961
ApaI GGGCCC 1 cut(s) 1531
ApeKI GCWGC 5 cut(s) 754, 757, 1565, 1689, 2628
ApoI RAATTY 8 cut(s) 642, 727, 790, 1119, 1275, 2016, 3091, 3631
Asp700I GAANNNNTTC 1 cut(s) 86
AspA2I CCTAGG 1 cut(s) 365
AspLEI GCGC 1 cut(s) 1493
AspS9I GGNCC 6 cut(s) 484, 1527, 1528, 1813, 3028, 3224
AsuC2I CCSGG 1 cut(s) 799
AsuHPI GGTGA 3 cut(s) 1042, 1819, 1945
AsuII TTCGAA 1 cut(s) 1297
AvaI CYCGRG 2 cut(s) 1785, 2368
AvaII GGWCC 3 cut(s) 484, 3028, 3224
AvrII CCTAGG 1 cut(s) 365
BaeGI GKGCMC 2 cut(s) 1531, 3530
BaeI ACNNNNGTAYC 2 cut(s) 2677, 2710
BalI TGGCCA 1 cut(s) 1963
BamHI GGATCC 2 cut(s) 667, 1221
BanI GGYRCC 1 cut(s) 3525
BanII GRGCYC 1 cut(s) 1531
BauI CACGAG 1 cut(s) 2310
BbrPI CACGTG 1 cut(s) 3236
BbsI GAAGAC 5 cut(s) 636, 1172, 1920, 2293, 3416
Bbv12I GWGCWC 1 cut(s) 1048
BbvI GCAGC 5 cut(s) 741, 769, 1552, 1676, 2640
BccI CCATC 6 cut(s) 1548, 2471, 2674, 3019, 3073, 3553
BceAI ACGGC 2 cut(s) 540, 1933
BcgI CGANNNNNNTGC 2 cut(s) 308, 342
BciT130I CCWGG 4 cut(s) 482, 2957, 2997, 3287
BciVI GTATCC 2 cut(s) 1105, 1860
BcnI CCSGG 1 cut(s) 799
BcoDI GTCTC 4 cut(s) 328, 1121, 1782, 3299
BfaI CTAG 9 cut(s) 366, 1482, 1778, 1827, 1845, 2577, 3197, 3624, 3640
BfmI CTRYAG 2 cut(s) 755, 3007
BfrI CTTAAG 1 cut(s) 65
BfuAI ACCTGC 2 cut(s) 1600, 2299
BfuI GTATCC 2 cut(s) 1105, 1860
BisI GCNGC 7 cut(s) 755, 758, 1348, 1566, 1690, 2629, 2632
BlnI CCTAGG 1 cut(s) 365
BlsI GCNGC 7 cut(s) 756, 759, 1349, 1567, 1691, 2630, 2633
BmcAI AGTACT 1 cut(s) 2704
Bme1390I CCNGG 5 cut(s) 482, 799, 2957, 2997, 3287
Bme18I GGWCC 3 cut(s) 484, 3028, 3224
BmeT110I CYCGRG 2 cut(s) 1785, 2368
BmgT120I GGNCC 6 cut(s) 484, 1527, 1528, 1813, 3028, 3224
BmiI GGNNCC 6 cut(s) 669, 1223, 1529, 2722, 3527, 3578
BmrFI CCNGG 5 cut(s) 482, 799, 2957, 2997, 3287
BmrI ACTGGG 2 cut(s) 1227, 2470
BmsI GCATC 9 cut(s) 338, 697, 832, 1292, 1592, 2331, 2551, 2580, 3344
BmuI ACTGGG 2 cut(s) 1227, 2470
BpiI GAAGAC 5 cut(s) 636, 1172, 1920, 2293, 3416
BpmI CTGGAG 1 cut(s) 2039
Bpu14I TTCGAA 1 cut(s) 1297
BpuEI CTTGAG 3 cut(s) 908, 2288, 2710
BpuMI CCSGG 1 cut(s) 799
BsaAI YACGTR 2 cut(s) 1472, 3236
BsaHI GRCGYC 3 cut(s) 213, 264, 315
BsaJI CCNNGG 4 cut(s) 365, 1353, 2821, 3286
BsaWI WCCGGW 3 cut(s) 2029, 2669, 2728
Bsc4I CCNNNNNNNGG 4 cut(s) 132, 1354, 3047, 3364
Bse118I RCCGGY 1 cut(s) 307
Bse1I ACTGG 8 cut(s) 60, 126, 210, 261, 1222, 2158, 2465, 2738
Bse3DI GCAATG 1 cut(s) 47
BseAI TCCGGA 1 cut(s) 2728
BseBI CCWGG 4 cut(s) 482, 2957, 2997, 3287
BseDI CCNNGG 4 cut(s) 365, 1353, 2821, 3286
BseLI CCNNNNNNNGG 4 cut(s) 132, 1354, 3047, 3364
BseMI GCAATG 1 cut(s) 47
BseMII CTCAG 4 cut(s) 2309, 2754, 3164, 3329
BseNI ACTGG 8 cut(s) 60, 126, 210, 261, 1222, 2158, 2465, 2738
BseRI GAGGAG 1 cut(s) 709
BseSI GKGCMC 2 cut(s) 1531, 3530
BseXI GCAGC 5 cut(s) 741, 769, 1552, 1676, 2640
BseYI CCCAGC 1 cut(s) 1144
BsgI GTGCAG 1 cut(s) 423
BshFI GGCC 4 cut(s) 1529, 1814, 1920, 1963
BshNI GGYRCC 1 cut(s) 3525
BsiHKAI GWGCWC 1 cut(s) 1048
BsiHKCI CYCGRG 2 cut(s) 1785, 2368
BsiSI CCGG 5 cut(s) 308, 798, 2030, 2670, 2729
BslFI GGGAC 1 cut(s) 2471
BslI CCNNNNNNNGG 4 cut(s) 132, 1354, 3047, 3364
BsmAI GTCTC 4 cut(s) 328, 1121, 1782, 3299
BsmFI GGGAC 1 cut(s) 2471
BsmI GAATGC 4 cut(s) 964, 1493, 1730, 2896
BsnI GGCC 4 cut(s) 1529, 1814, 1920, 1963
BsoBI CYCGRG 2 cut(s) 1785, 2368
Bsp119I TTCGAA 1 cut(s) 1297
Bsp120I GGGCCC 1 cut(s) 1527
Bsp1286I GDGCHC 3 cut(s) 1048, 1531, 3530
Bsp13I TCCGGA 1 cut(s) 2728
Bsp1407I TGTACA 2 cut(s) 2433, 3462
Bsp19I CCATGG 2 cut(s) 1353, 2821
BspACI CCGC 3 cut(s) 1348, 1924, 2631
BspANI GGCC 4 cut(s) 1529, 1814, 1920, 1963
BspCNI CTCAG 4 cut(s) 2308, 2755, 3165, 3328
BspEI TCCGGA 1 cut(s) 2728
BspHI TCATGA 1 cut(s) 954
BspLI GGNNCC 6 cut(s) 669, 1223, 1529, 2722, 3527, 3578
BspMAI CTGCAG 2 cut(s) 759, 3011
BspMI ACCTGC 2 cut(s) 1600, 2299
BspPI GGATC 8 cut(s) 662, 675, 1216, 1229, 2089, 2148, 3007, 3590
BspQI GCTCTTC 1 cut(s) 1174
BspT104I TTCGAA 1 cut(s) 1297
BspT107I GGYRCC 1 cut(s) 3525
BspTI CTTAAG 1 cut(s) 65
BsrBI CCGCTC 1 cut(s) 1350
BsrDI GCAATG 1 cut(s) 47
BsrFI RCCGGY 1 cut(s) 307
BsrGI TGTACA 2 cut(s) 2433, 3462
BsrI ACTGG 8 cut(s) 60, 126, 210, 261, 1222, 2158, 2465, 2738
BssAI RCCGGY 1 cut(s) 307
BssECI CCNNGG 4 cut(s) 365, 1353, 2821, 3286
BssNI GRCGYC 3 cut(s) 213, 264, 315
BssSI CACGAG 1 cut(s) 2310
BssT1I CCWWGG 3 cut(s) 365, 1353, 2821
Bst2BI CACGAG 1 cut(s) 2310
Bst2UI CCWGG 4 cut(s) 482, 2957, 2997, 3287
Bst4CI ACNGT 3 cut(s) 149, 2433, 3415
Bst6I CTCTTC 8 cut(s) 339, 583, 686, 1174, 1244, 2028, 2931, 3302
BstACI GRCGYC 3 cut(s) 213, 264, 315
BstAFI CTTAAG 1 cut(s) 65
BstAUI TGTACA 2 cut(s) 2433, 3462
BstBAI YACGTR 2 cut(s) 1472, 3236
BstBI TTCGAA 1 cut(s) 1297
BstC8I GCNNGC 5 cut(s) 1186, 1627, 1730, 1832, 1922
BstDEI CTNAG 9 cut(s) 602, 717, 2144, 2276, 2295, 2763, 3173, 3206, 3315
BstDSI CCRYGG 2 cut(s) 1353, 2821
BstHHI GCGC 1 cut(s) 1493
BstMAI GTCTC 4 cut(s) 328, 1121, 1782, 3299
BstMWI GCNNNNNNNGC 3 cut(s) 926, 1347, 1514
BstNI CCWGG 4 cut(s) 482, 2957, 2997, 3287
BstNSI RCATGY 4 cut(s) 1629, 1777, 2440, 3134
BstSCI CCNGG 5 cut(s) 480, 797, 2955, 2995, 3285
BstSFI CTRYAG 2 cut(s) 755, 3007
BstSLI GKGCMC 2 cut(s) 1531, 3530
BstV1I GCAGC 5 cut(s) 741, 769, 1552, 1676, 2640
BstV2I GAAGAC 5 cut(s) 636, 1172, 1920, 2293, 3416
BstX2I RGATCY 5 cut(s) 667, 1221, 2094, 2140, 2999
BstYI RGATCY 5 cut(s) 667, 1221, 2094, 2140, 2999
BsuI GTATCC 2 cut(s) 1105, 1860
BsuRI GGCC 4 cut(s) 1529, 1814, 1920, 1963
BtgI CCRYGG 2 cut(s) 1353, 2821
BtgZI GCGATG 1 cut(s) 17
BtsI GCAGTG 2 cut(s) 767, 2641
BtsIMutI CAGTG 7 cut(s) 133, 767, 809, 1809, 2151, 2359, 2641
BveI ACCTGC 2 cut(s) 1600, 2299
Cac8I GCNNGC 5 cut(s) 1186, 1627, 1730, 1832, 1922
CciI TCATGA 1 cut(s) 954
CfoI GCGC 1 cut(s) 1493
Cfr10I RCCGGY 1 cut(s) 307
Cfr13I GGNCC 6 cut(s) 484, 1527, 1528, 1813, 3028, 3224
CseI GACGC 2 cut(s) 1850, 3503
Csp6I GTAC 5 cut(s) 680, 2434, 2703, 3203, 3463
CviQI GTAC 5 cut(s) 680, 2434, 2703, 3203, 3463
DdeI CTNAG 9 cut(s) 602, 717, 2144, 2276, 2295, 2763, 3173, 3206, 3315
DraI TTTAAA 1 cut(s) 1153
DraIII CACNNNGTG 1 cut(s) 588
DrdI GACNNNNNNGTC 2 cut(s) 320, 2738
DseDI GACNNNNNNGTC 2 cut(s) 320, 2738
EaeI YGGCCR 2 cut(s) 1918, 1961
Eam1104I CTCTTC 8 cut(s) 339, 583, 686, 1174, 1244, 2028, 2931, 3302
EarI CTCTTC 8 cut(s) 339, 583, 686, 1174, 1244, 2028, 2931, 3302
Eco130I CCWWGG 3 cut(s) 365, 1353, 2821
Eco24I GRGCYC 1 cut(s) 1531
Eco32I GATATC 1 cut(s) 2406
Eco47I GGWCC 3 cut(s) 484, 3028, 3224
Eco57I CTGAAG 4 cut(s) 379, 1623, 1932, 2987
Eco72I CACGTG 1 cut(s) 3236
Eco88I CYCGRG 2 cut(s) 1785, 2368
EcoO109I RGGNCCY 4 cut(s) 484, 1527, 1528, 1813
EcoRI GAATTC 3 cut(s) 642, 1275, 3631
EcoRII CCWGG 4 cut(s) 480, 2955, 2995, 3285
EcoRV GATATC 1 cut(s) 2406
EcoT14I CCWWGG 3 cut(s) 365, 1353, 2821
EcoT22I ATGCAT 1 cut(s) 1631
EcoT38I GRGCYC 1 cut(s) 1531
ErhI CCWWGG 3 cut(s) 365, 1353, 2821
FalI AAGNNNNNCTT 2 cut(s) 1420, 1452
FaqI GGGAC 1 cut(s) 2471
FauNDI CATATG 2 cut(s) 2902, 3215
FblI GTMKAC 3 cut(s) 210, 261, 1498
Fnu4HI GCNGC 7 cut(s) 755, 758, 1348, 1566, 1690, 2629, 2632
FriOI GRGCYC 1 cut(s) 1531
Fsp4HI GCNGC 7 cut(s) 755, 758, 1348, 1566, 1690, 2629, 2632
FspBI CTAG 9 cut(s) 366, 1482, 1778, 1827, 1845, 2577, 3197, 3624, 3640
GlaI GCGC 1 cut(s) 1492
GluI GCNGC 7 cut(s) 755, 758, 1348, 1566, 1690, 2629, 2632
GsaI CCCAGC 1 cut(s) 1148
GsuI CTGGAG 1 cut(s) 2039
HaeIII GGCC 4 cut(s) 1529, 1814, 1920, 1963
HapII CCGG 5 cut(s) 308, 798, 2030, 2670, 2729
HgaI GACGC 2 cut(s) 1850, 3503
HhaI GCGC 1 cut(s) 1493
Hin1I GRCGYC 3 cut(s) 213, 264, 315
Hin6I GCGC 1 cut(s) 1491
HinP1I GCGC 1 cut(s) 1491
HincII GTYRAC 5 cut(s) 211, 262, 313, 2328, 2743
HindII GTYRAC 5 cut(s) 211, 262, 313, 2328, 2743
HindIII AAGCTT 2 cut(s) 2202, 2264
HpaII CCGG 5 cut(s) 308, 798, 2030, 2670, 2729
HphI GGTGA 3 cut(s) 1042, 1819, 1945
Hpy166II GTNNAC 8 cut(s) 211, 262, 313, 1499, 2328, 2375, 2608, 2743
Hpy8I GTNNAC 8 cut(s) 211, 262, 313, 1499, 2328, 2375, 2608, 2743
Hpy99I CGWCG 6 cut(s) 215, 218, 266, 269, 320, 1575
HpyCH4III ACNGT 3 cut(s) 149, 2433, 3415
HpyCH4IV ACGT 8 cut(s) 117, 213, 264, 315, 1471, 2377, 2773, 3235
HpyF10VI GCNNNNNNNGC 3 cut(s) 926, 1347, 1514
HpyF3I CTNAG 9 cut(s) 602, 717, 2144, 2276, 2295, 2763, 3173, 3206, 3315
HpySE526I ACGT 8 cut(s) 117, 213, 264, 315, 1471, 2377, 2773, 3235
Hsp92I GRCGYC 3 cut(s) 213, 264, 315
HspAI GCGC 1 cut(s) 1491
Kpn2I TCCGGA 1 cut(s) 2728
LguI GCTCTTC 1 cut(s) 1174
LmnI GCTCC 5 cut(s) 367, 1043, 1355, 1407, 3235
Lsp1109I GCAGC 5 cut(s) 741, 769, 1552, 1676, 2640
LweI GCATC 9 cut(s) 338, 697, 832, 1292, 1592, 2331, 2551, 2580, 3344
MaeI CTAG 9 cut(s) 366, 1482, 1778, 1827, 1845, 2577, 3197, 3624, 3640
MaeII ACGT 8 cut(s) 117, 213, 264, 315, 1471, 2377, 2773, 3235
MaeIII GTNAC 7 cut(s) 106, 158, 979, 2307, 2798, 2829, 2988
MbiI CCGCTC 1 cut(s) 1350
MfeI CAATTG 2 cut(s) 1503, 3329
MflI RGATCY 5 cut(s) 667, 1221, 2094, 2140, 2999
MhlI GDGCHC 3 cut(s) 1048, 1531, 3530
MlsI TGGCCA 1 cut(s) 1963
MluNI TGGCCA 1 cut(s) 1963
MlyI GAGTC 2 cut(s) 2701, 3244
MmeI TCCRAC 4 cut(s) 994, 1021, 1794, 2962
Mox20I TGGCCA 1 cut(s) 1963
Mph1103I ATGCAT 1 cut(s) 1631
MroI TCCGGA 1 cut(s) 2728
MroXI GAANNNNTTC 1 cut(s) 86
MscI TGGCCA 1 cut(s) 1963
MslI CAYNNNNRTG 2 cut(s) 95, 2807
Msp20I TGGCCA 1 cut(s) 1963
MspA1I CMGCKG 2 cut(s) 1924, 2631
MspCI CTTAAG 1 cut(s) 65
MspI CCGG 5 cut(s) 308, 798, 2030, 2670, 2729
MspR9I CCNGG 5 cut(s) 482, 799, 2957, 2997, 3287
MunI CAATTG 2 cut(s) 1503, 3329
Mva1269I GAATGC 4 cut(s) 964, 1493, 1730, 2896
MvaI CCWGG 4 cut(s) 482, 2957, 2997, 3287
MwoI GCNNNNNNNGC 3 cut(s) 926, 1347, 1514
NciI CCSGG 1 cut(s) 799
NcoI CCATGG 2 cut(s) 1353, 2821
NdeI CATATG 2 cut(s) 2902, 3215
NlaIV GGNNCC 6 cut(s) 669, 1223, 1529, 2722, 3527, 3578
NmuCI GTSAC 2 cut(s) 106, 2307
NsiI ATGCAT 1 cut(s) 1631
NspI RCATGY 4 cut(s) 1629, 1777, 2440, 3134
NspV TTCGAA 1 cut(s) 1297
PaeI GCATGC 1 cut(s) 1629
PagI TCATGA 1 cut(s) 954
PciI ACATGT 1 cut(s) 3130
PciSI GCTCTTC 1 cut(s) 1174
PctI GAATGC 4 cut(s) 964, 1493, 1730, 2896
PdmI GAANNNNTTC 1 cut(s) 86
PfeI GAWTC 9 cut(s) 353, 459, 536, 1400, 1766, 1823, 2272, 2364, 2659
PkrI GCNGC 7 cut(s) 756, 759, 1349, 1567, 1691, 2630, 2633
PleI GAGTC 2 cut(s) 2700, 3244
PmaCI CACGTG 1 cut(s) 3236
PmlI CACGTG 1 cut(s) 3236
PpsI GAGTC 2 cut(s) 2700, 3244
Ppu21I YACGTR 2 cut(s) 1472, 3236
PpuMI RGGWCCY 1 cut(s) 484
PscI ACATGT 1 cut(s) 3130
PsiI TTATAA 2 cut(s) 819, 2778
Psp5II RGGWCCY 1 cut(s) 484
Psp6I CCWGG 4 cut(s) 480, 2955, 2995, 3285
PspCI CACGTG 1 cut(s) 3236
PspFI CCCAGC 1 cut(s) 1144
PspGI CCWGG 4 cut(s) 480, 2955, 2995, 3285
PspN4I GGNNCC 6 cut(s) 669, 1223, 1529, 2722, 3527, 3578
PspOMI GGGCCC 1 cut(s) 1527
PspPI GGNCC 6 cut(s) 484, 1527, 1528, 1813, 3028, 3224
PspPPI RGGWCCY 1 cut(s) 484
PstI CTGCAG 2 cut(s) 759, 3011
PsuI RGATCY 5 cut(s) 667, 1221, 2094, 2140, 2999
RsaI GTAC 5 cut(s) 681, 2435, 2704, 3204, 3464
RsaNI GTAC 5 cut(s) 680, 2434, 2703, 3203, 3463
RseI CAYNNNNRTG 2 cut(s) 95, 2807
SalI GTCGAC 2 cut(s) 209, 260
SapI GCTCTTC 1 cut(s) 1174
SatI GCNGC 7 cut(s) 755, 758, 1348, 1566, 1690, 2629, 2632
Sau96I GGNCC 6 cut(s) 484, 1527, 1528, 1813, 3028, 3224
ScaI AGTACT 1 cut(s) 2704
SchI GAGTC 2 cut(s) 2701, 3244
ScrFI CCNGG 5 cut(s) 482, 799, 2957, 2997, 3287
SduI GDGCHC 3 cut(s) 1048, 1531, 3530
SfaNI GCATC 9 cut(s) 338, 697, 832, 1292, 1592, 2331, 2551, 2580, 3344
SfcI CTRYAG 2 cut(s) 755, 3007
SfuI TTCGAA 1 cut(s) 1297
SinI GGWCC 3 cut(s) 484, 3028, 3224
SmiMI CAYNNNNRTG 2 cut(s) 95, 2807
SmlI CTYRAG 4 cut(s) 65, 887, 2267, 2689
SmoI CTYRAG 4 cut(s) 65, 887, 2267, 2689
SphI GCATGC 1 cut(s) 1629
SsiI CCGC 3 cut(s) 1348, 1924, 2631
SspI AATATT 3 cut(s) 1896, 2444, 2500
SspMI CTAG 9 cut(s) 366, 1482, 1778, 1827, 1845, 2577, 3197, 3624, 3640
StyD4I CCNGG 5 cut(s) 480, 797, 2955, 2995, 3285
StyI CCWWGG 3 cut(s) 365, 1353, 2821
TaaI ACNGT 3 cut(s) 149, 2433, 3415
TaiI ACGT 8 cut(s) 120, 216, 267, 318, 1474, 2380, 2776, 3238
TaqI TCGA 9 cut(s) 210, 261, 318, 1297, 1523, 1534, 1570, 2592, 3378
TatI WGTACW 4 cut(s) 2433, 2702, 3202, 3462
TauI GCSGC 2 cut(s) 1350, 2634
TfiI GAWTC 9 cut(s) 353, 459, 536, 1400, 1766, 1823, 2272, 2364, 2659
TscAI CASTG 7 cut(s) 133, 767, 816, 1809, 2158, 2359, 2641
TseFI GTSAC 2 cut(s) 106, 2307
TseI GCWGC 5 cut(s) 754, 757, 1565, 1689, 2628
Tsp45I GTSAC 2 cut(s) 106, 2307
TspGWI ACGGA 5 cut(s) 1211, 1566, 1869, 2382, 3020
TspRI CASTG 7 cut(s) 133, 767, 816, 1809, 2158, 2359, 2641
Vha464I CTTAAG 1 cut(s) 65
VpaK11BI GGWCC 3 cut(s) 484, 3028, 3224
XapI RAATTY 8 cut(s) 642, 727, 790, 1119, 1275, 2016, 3091, 3631
XbaI TCTAGA 1 cut(s) 3196
XceI RCATGY 4 cut(s) 1629, 1777, 2440, 3134
XcmI CCANNNNNNNNNTGG 2 cut(s) 589, 3072
XmaJI CCTAGG 1 cut(s) 365
XmiI GTMKAC 3 cut(s) 210, 261, 1498
XmnI GAANNNNTTC 1 cut(s) 86
XspI CTAG 9 cut(s) 366, 1482, 1778, 1827, 1845, 2577, 3197, 3624, 3640
ZraI GACGTC 3 cut(s) 214, 265, 316
ZrmI AGTACT 1 cut(s) 2704
Zsp2I ATGCAT 1 cut(s) 1631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.