Rw5G039610

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000085
Physical Location & Seq
Forward (+)
69856028 .. 69858814
2787 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G039610.1

Sequence Viewer

Length: 2787 bp
ATGGATGAGCTGATTAAGGCGCTGATAGATGATGAGGTCACCATCGCTGGGGTTTATGGCATGGGAGGTGTGGGCAAGACAACCATGGTGAGAAATGTTGCTCTACAAGCACAAAAAAATGGGCTCTTTGATCATGTGATTGTGGCTGTCGTATCCCAAGACCCCAACTTTAGAAGGATTCAACAGCAATTGGCAGAGCAGCTCGACTTGAGATTGGAGGGGACGGAATCTGGAACAGCCGATAGATTGAAGGAGAGGATAACGGAAGGAAAGAGGATCCTTATAATCTTGGACGACATTTGGGAAACCATAGACTTGTCACTTATAGGAATTCCCAACCCTGATGAGCTTAAGCGATGCAACTCTAAAGTCCTAATCACCACAAGAAGATTGGATGTTTGCCGCTCCATGAAGAGCCAAGCAAGTTCTCTTCTCAGTATACTATCTAAAGAAGATTCTTGGAAACTTTTTGTGGATTGTTCGAGAATATCTTTTGATGAATCTACCAATATCTATCGAGCGAGGGCGATAGTTAGAGAATGTGGTGGTCTGCCCGTTGCATTGAAAGCAATTGCACGGGCACTTGAAGATAAAGACATGGCTGAATGGAATATAGCAGTTCAACAATTGAGGGCATCTCAAATTGCCAGTCTTGATGATCAGGGTCCTGCGTTCAAATGTATAAAGTTAAGCTATGACTACTTGAGATCTGATGATGCCAAATCATTCTTCTTGCTTTGCTCCCTATTCCCAGAAGACTATGATATCCCTATCAAAGACTTGTTCAAGTATGCTATCGGGAACGGATTGCTTCGAGAAGCCGCCACAATCCAAGAAGCCAGAGATAGAGCAAATTCGGTGATCAAGTACCTCAAAGCTTCCAGCTTACTTTTGGACAGTCCAAAGGAGGGATGCGTAAAGATGCATGATGTTGTTCGGGATACAGCCAAGTCAATTCCAAGGTATGAGGATGATCATGGATTTTGCGTTAAAGCTGGTTGTGGATTAAAGGAGTTTCGAGGGACCACTGGGCATGGAGACTGCTCTGCCATCTCACTAATGATGAATGATATTGATCGGCTTCCTGAAGAGTTGGTATGTCCAAAACTCCAGATTTTGTTACTACAAAACAATGTTCATATAGATGAGATCCCAGAATCTTTTTTTCGAAGTTCGAAGGCACTGAGGGTCTTAGATATTAGCTTCTCTAGAATTTCATCACTACCCAGGTCATTTAGTTTCCTAACCAACCTCCGAGCTTTGTTTTTAGACCATTGCAATGAGTTGGTTGACATATCAATATTCGGAAACTTGAAGAGACTTGAGATTCTTAGTATGATAGAATTTCCTCTCAAGGAGTTACCAAAAGAGATGGGAAATTTGACCAATCTAAAGATGCTGGACTTAAGTGGGAGTTACAATATTGGTATATTTCCATATAGTGTGTTGTCAAGATTGTGTAAATTAGAAGAACTGTACATGCAATGTAATTCTGGGAACTGGGGAAGCAAAATTCATGGAGGAGAAGAAACTAATGTTGGTCCTGATAAGTTGATTGGCTTGCCACTTTTAAACACCGTGAAGCTTCGCATATCTGATGCAGAATGCTTGCCTAAAAATCTGTTCTGGGATTGGCATTTGGTTACCTTTGATATATGTATCAGCAGAGAACCACCATCTAGAGACTACTCTTCAGAACCAATTTTCTCACACGATCATTCAAGGACCCTGACTCTTAACACAACAATCAACAGCTTACCAGATTGGTTTATCAACACAGTGACAGAGAAAGCAGTGAAACTACAGTACCTAAAGTGCAGGGGATTAAAGAACATTCTTGTGGAACATAACAGAGGCAGATTACATGGACTGAAGAATCTTGCTGTAATTGGTCCACATGAGAACTTGGAAGTGTTGATGGAAACAATAACACTGGTGCCGAATAAACCTGTGTTTGAGAAGTTGGAAGAGTTGCATCTCCTTGGTGTGAGTTGCCTGAAGGAATTATGTGTTGGTGAATTACCCGTTGGGTCTCTATGCAATCTCAAGTTATTCAAGGTAGAATATTGCCATGACTTGGTAAAGACACTCTTACCATCAAATTTGTGGCAGAGACTACACAATATGGAAAAACTAATTTGTGAAGATATGAATCAAATGGAGTATGTATTTGGATCCAAAGGGATGGAGTCAGAAGATATTATCTTGATAAAATTGAGGGAGATGATATTGTGGAATCTAGAGAATCTAATAGGCATATGCAATGGTCCTGCTCCAAATGCAGTCTTCCATAGTCTTAAAAGTTTGGCAGTATATGGGTGCAAGAAACTGAAACATCTGTTCACATACGATATAGCTCACTGTCTTTTGCATTTGGAATACCTTTCGGTGACTCAGTGTTCTAGCTTGGACAGGGTTATCGAACCTAGCAAAGAAACAGTCAACAAGAAGATGGTTTTTCCTGAATTGAAAAACTTAGCTTTGTCATATCTTCCACAGCTCACAACGTTCTGCAGTAGTGAAAGTGCTACCATTGAGTGCCCTTCATTGGAACACTTGCATGTGCAGGACTGCCCCCATTTTACAACCTCTATTTCTGACTTCCACAGCGGGAATCGATTCATCCAAGTCAATGATCTACGACATTTGAGGTATAATGAACTCATTAGTCGGTATACTGCTTTGCCTTTTTTCTTTTTCTTTTTCTTTTTGCACAAGTATGTTTCTGAGGATATATGCTCCGCAATTTGGAGTTATAATTCTGATTGCATTCTTAAGTTCGATTAA

Protein Analysis

928

Amino Acids

105.36

Weight (kDa)

6.0

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 1 - 158 5.2e-33 NB-ARC domain
AAA_22 PF13401 18 - 100 4.8e-06 AAA domain
LRR_8 PF13855 368 - 427 2.9e-10 Leucine rich repeat
LRR_14 PF23598 370 - 497 8.1e-12 Leucine-rich repeat region
LRR_RPS2 PF23247 655 - 800 2.1e-26 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 758 - 863 2.1e-16 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 284, 2757
AccB1I GGYRCC 1 cut(s) 1936
AccB7I CCANNNNNTGG 1 cut(s) 2077
AccBSI CCGCTC 1 cut(s) 405
AccI GTMKAC 2 cut(s) 439, 2675
AciI CCGC 4 cut(s) 403, 822, 2610, 2742
AclI AACGTT 1 cut(s) 2507
AclWI GGATC 5 cut(s) 271, 284, 1144, 2169, 2182
AcsI RAATTY 7 cut(s) 330, 853, 1212, 1343, 1378, 1512, 2101
AcuI CTGAAG 4 cut(s) 1107, 1677, 1892, 2018
AfaI GTAC 3 cut(s) 869, 1478, 1808
AfiI CCNNNNNNNGG 5 cut(s) 48, 908, 2077, 2548, 2748
AflII CTTAAG 3 cut(s) 350, 1405, 2774
AjnI CCWGG 1 cut(s) 1226
AjuI GAANNNNNNNTTGG 8 cut(s) 713, 745, 1521, 1553, 1995, 2010, 2027, 2042
Alw26I GTCTC 5 cut(s) 1032, 1312, 1677, 2037, 2107
AlwI GGATC 5 cut(s) 271, 284, 1144, 2169, 2182
ApeKI GCWGC 1 cut(s) 199
ApoI RAATTY 7 cut(s) 330, 853, 1212, 1343, 1378, 1512, 2101
ArsI GACNNNNNNTTYG 4 cut(s) 634, 666, 2424, 2456
Asp700I GAANNNNTTC 1 cut(s) 2618
AspLEI GCGC 1 cut(s) 22
AspS9I GGNCC 6 cut(s) 665, 1023, 1541, 1725, 1892, 2267
AsuHPI GGTGA 6 cut(s) 31, 100, 370, 871, 2027, 2401
AsuII TTCGAA 2 cut(s) 1168, 1175
AvaII GGWCC 6 cut(s) 665, 1023, 1541, 1725, 1892, 2267
BaeGI GKGCMC 2 cut(s) 583, 2543
BaeI ACNNNNGTAYC 2 cut(s) 1790, 1823
BamHI GGATCC 2 cut(s) 276, 2174
BanI GGYRCC 1 cut(s) 1936
BanII GRGCYC 1 cut(s) 126
BbsI GAAGAC 2 cut(s) 762, 2278
BbvI GCAGC 1 cut(s) 211
BccI CCATC 8 cut(s) 50, 1058, 1366, 1684, 1912, 2104, 2179, 2446
BciT130I CCWGG 1 cut(s) 1228
BciVI GTATCC 2 cut(s) 163, 934
BclI TGATCA 4 cut(s) 130, 658, 861, 973
BcoDI GTCTC 5 cut(s) 1032, 1312, 1677, 2037, 2107
BfaI CTAG 5 cut(s) 1209, 1680, 2240, 2403, 2427
BfmI CTRYAG 2 cut(s) 1802, 2512
BfoI RGCGCY 1 cut(s) 23
BfrI CTTAAG 3 cut(s) 350, 1405, 2774
BfuI GTATCC 2 cut(s) 163, 934
BglII AGATCT 1 cut(s) 707
BisI GCNGC 3 cut(s) 200, 403, 822
BlsI GCNGC 3 cut(s) 201, 404, 823
Bme1390I CCNGG 1 cut(s) 1228
Bme18I GGWCC 6 cut(s) 665, 1023, 1541, 1725, 1892, 2267
BmgT120I GGNCC 6 cut(s) 665, 1023, 1541, 1725, 1892, 2267
BmiI GGNNCC 6 cut(s) 278, 666, 1024, 1727, 1938, 2176
BmrFI CCNGG 1 cut(s) 1228
BmrI ACTGGG 2 cut(s) 1038, 1510
BmsI GCATC 8 cut(s) 347, 644, 706, 902, 912, 1386, 1588, 1984
BmuI ACTGGG 2 cut(s) 1038, 1510
BpiI GAAGAC 2 cut(s) 762, 2278
BplI GAGNNNNNCTC 2 cut(s) 622, 654
BpmI CTGGAG 1 cut(s) 1094
Bpu14I TTCGAA 2 cut(s) 1168, 1175
BpuEI CTTGAG 5 cut(s) 229, 724, 1337, 1343, 2030
Bsa29I ATCGAT 1 cut(s) 2617
BsaBI GATNNNNATC 2 cut(s) 1074, 2151
BsaI GGTCTC 1 cut(s) 2037
BsaJI CCNNGG 4 cut(s) 84, 959, 1226, 1981
Bsc4I CCNNNNNNNGG 5 cut(s) 48, 908, 2077, 2548, 2748
Bse1I ACTGG 4 cut(s) 648, 1033, 1505, 1938
Bse3DI GCAATG 4 cut(s) 1273, 1285, 1490, 2269
Bse8I GATNNNNATC 2 cut(s) 1074, 2151
BseBI CCWGG 1 cut(s) 1228
BseCI ATCGAT 1 cut(s) 2617
BseDI CCNNGG 4 cut(s) 84, 959, 1226, 1981
BseGI GGATG 6 cut(s) 10, 400, 917, 976, 2190, 2622
BseJI GATNNNNATC 2 cut(s) 1074, 2151
BseLI CCNNNNNNNGG 5 cut(s) 48, 908, 2077, 2548, 2748
BseMI GCAATG 4 cut(s) 1273, 1285, 1490, 2269
BseMII CTCAG 4 cut(s) 448, 1175, 2408, 2718
BseNI ACTGG 4 cut(s) 648, 1033, 1505, 1938
BseRI GAGGAG 1 cut(s) 1536
BseSI GKGCMC 2 cut(s) 583, 2543
BseXI GCAGC 1 cut(s) 211
BseYI CCCAGC 1 cut(s) 47
BsgI GTGCAG 2 cut(s) 1837, 2585
BshNI GGYRCC 1 cut(s) 1936
BshVI ATCGAT 1 cut(s) 2617
BslFI GGGAC 2 cut(s) 235, 1036
BslI CCNNNNNNNGG 5 cut(s) 48, 908, 2077, 2548, 2748
BsmAI GTCTC 5 cut(s) 1032, 1312, 1677, 2037, 2107
BsmFI GGGAC 2 cut(s) 235, 1036
BsmI GAATGC 2 cut(s) 1610, 2769
Bso31I GGTCTC 1 cut(s) 2037
Bsp119I TTCGAA 2 cut(s) 1168, 1175
Bsp1286I GDGCHC 3 cut(s) 126, 583, 2543
Bsp1407I TGTACA 1 cut(s) 1476
Bsp19I CCATGG 1 cut(s) 84
BspACI CCGC 4 cut(s) 403, 822, 2610, 2742
BspCNI CTCAG 4 cut(s) 447, 1176, 2407, 2719
BspDI ATCGAT 1 cut(s) 2617
BspLI GGNNCC 6 cut(s) 278, 666, 1024, 1727, 1938, 2176
BspMAI CTGCAG 1 cut(s) 2516
BspPI GGATC 5 cut(s) 271, 284, 1144, 2169, 2182
BspQI GCTCTTC 1 cut(s) 407
BspT104I TTCGAA 2 cut(s) 1168, 1175
BspT107I GGYRCC 1 cut(s) 1936
BspTI CTTAAG 3 cut(s) 350, 1405, 2774
BspTNI GGTCTC 1 cut(s) 2037
BsrBI CCGCTC 1 cut(s) 405
BsrDI GCAATG 4 cut(s) 1273, 1285, 1490, 2269
BsrGI TGTACA 1 cut(s) 1476
BsrI ACTGG 4 cut(s) 648, 1033, 1505, 1938
BssECI CCNNGG 4 cut(s) 84, 959, 1226, 1981
BssNAI GTATAC 2 cut(s) 440, 2676
BssT1I CCWWGG 3 cut(s) 84, 959, 1981
Bst1107I GTATAC 2 cut(s) 440, 2676
Bst2UI CCWGG 1 cut(s) 1228
Bst4CI ACNGT 7 cut(s) 899, 1476, 1579, 1780, 1806, 2363, 2440
Bst6I CTCTTC 6 cut(s) 407, 435, 1083, 1310, 1696, 1962
BstAFI CTTAAG 3 cut(s) 350, 1405, 2774
BstAUI TGTACA 1 cut(s) 1476
BstBI TTCGAA 2 cut(s) 1168, 1175
BstC8I GCNNGC 2 cut(s) 1562, 1610
BstDEI CTNAG 7 cut(s) 434, 1184, 1192, 1331, 2394, 2476, 2727
BstDSI CCRYGG 1 cut(s) 84
BstEII GGTNACC 2 cut(s) 37, 1642
BstF5I GGATG 6 cut(s) 10, 400, 917, 976, 2190, 2622
BstH2I RGCGCY 1 cut(s) 23
BstHHI GCGC 1 cut(s) 22
BstMAI GTCTC 5 cut(s) 1032, 1312, 1677, 2037, 2107
BstMWI GCNNNNNNNGC 3 cut(s) 107, 566, 2279
BstNI CCWGG 1 cut(s) 1228
BstNSI RCATGY 2 cut(s) 1483, 2564
BstPI GGTNACC 2 cut(s) 37, 1642
BstSCI CCNGG 1 cut(s) 1226
BstSFI CTRYAG 2 cut(s) 1802, 2512
BstSLI GKGCMC 2 cut(s) 583, 2543
BstV1I GCAGC 1 cut(s) 211
BstV2I GAAGAC 2 cut(s) 762, 2278
BstX2I RGATCY 4 cut(s) 276, 707, 1149, 2174
BstXI CCANNNNNNTGG 1 cut(s) 2185
BstYI RGATCY 4 cut(s) 276, 707, 1149, 2174
BstZ17I GTATAC 2 cut(s) 440, 2676
Bsu15I ATCGAT 1 cut(s) 2617
BsuI GTATCC 2 cut(s) 163, 934
BsuTUI ATCGAT 1 cut(s) 2617
BtgI CCRYGG 1 cut(s) 84
BtgZI GCGATG 2 cut(s) 28, 370
BtsCI GGATG 6 cut(s) 10, 400, 917, 976, 2190, 2622
BtsI GCAGTG 1 cut(s) 1800
BtsIMutI CAGTG 7 cut(s) 1026, 1181, 1785, 1800, 1931, 2359, 2402
Cac8I GCNNGC 2 cut(s) 1562, 1610
CfoI GCGC 1 cut(s) 22
Cfr13I GGNCC 6 cut(s) 665, 1023, 1541, 1725, 1892, 2267
ClaI ATCGAT 1 cut(s) 2617
Csp6I GTAC 3 cut(s) 868, 1477, 1807
CviQI GTAC 3 cut(s) 868, 1477, 1807
DdeI CTNAG 7 cut(s) 434, 1184, 1192, 1331, 2394, 2476, 2727
DraI TTTAAA 1 cut(s) 1572
Eam1104I CTCTTC 6 cut(s) 407, 435, 1083, 1310, 1696, 1962
EarI CTCTTC 6 cut(s) 407, 435, 1083, 1310, 1696, 1962
Eco130I CCWWGG 3 cut(s) 84, 959, 1981
Eco24I GRGCYC 1 cut(s) 126
Eco31I GGTCTC 1 cut(s) 2037
Eco32I GATATC 1 cut(s) 766
Eco47I GGWCC 6 cut(s) 665, 1023, 1541, 1725, 1892, 2267
Eco57I CTGAAG 4 cut(s) 1107, 1677, 1892, 2018
Eco91I GGTNACC 2 cut(s) 37, 1642
EcoO109I RGGNCCY 2 cut(s) 665, 1725
EcoO65I GGTNACC 2 cut(s) 37, 1642
EcoRI GAATTC 1 cut(s) 330
EcoRII CCWGG 1 cut(s) 1226
EcoRV GATATC 1 cut(s) 766
EcoT14I CCWWGG 3 cut(s) 84, 959, 1981
EcoT22I ATGCAT 1 cut(s) 927
EcoT38I GRGCYC 1 cut(s) 126
ErhI CCWWGG 3 cut(s) 84, 959, 1981
FalI AAGNNNNNCTT 2 cut(s) 2075, 2107
FaqI GGGAC 2 cut(s) 235, 1036
FauI CCCGC 1 cut(s) 2603
FauNDI CATATG 1 cut(s) 2258
FbaI TGATCA 4 cut(s) 130, 658, 861, 973
FblI GTMKAC 2 cut(s) 439, 2675
Fnu4HI GCNGC 3 cut(s) 200, 403, 822
FokI GGATG 6 cut(s) 17, 407, 924, 983, 2197, 2609
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 3 cut(s) 200, 403, 822
FspBI CTAG 5 cut(s) 1209, 1680, 2240, 2403, 2427
GlaI GCGC 1 cut(s) 21
GluI GCNGC 3 cut(s) 200, 403, 822
GsaI CCCAGC 1 cut(s) 51
GsuI CTGGAG 1 cut(s) 1094
HaeII RGCGCY 1 cut(s) 23
HhaI GCGC 1 cut(s) 22
Hin6I GCGC 1 cut(s) 20
HinP1I GCGC 1 cut(s) 20
HincII GTYRAC 2 cut(s) 1291, 2443
HindII GTYRAC 2 cut(s) 1291, 2443
HindIII AAGCTT 2 cut(s) 876, 1583
HphI GGTGA 6 cut(s) 31, 100, 370, 871, 2027, 2401
Hpy166II GTNNAC 6 cut(s) 440, 1291, 1895, 2343, 2443, 2676
Hpy188I TCNGA 9 cut(s) 712, 1256, 1307, 1597, 1696, 2194, 2599, 2728, 2764
Hpy8I GTNNAC 6 cut(s) 440, 1291, 1895, 2343, 2443, 2676
HpyAV CCTTC 6 cut(s) 168, 244, 260, 1171, 1993, 2553
HpyCH4III ACNGT 7 cut(s) 899, 1476, 1579, 1780, 1806, 2363, 2440
HpyCH4IV ACGT 1 cut(s) 2507
HpyF10VI GCNNNNNNNGC 3 cut(s) 107, 566, 2279
HpyF3I CTNAG 7 cut(s) 434, 1184, 1192, 1331, 2394, 2476, 2727
HpySE526I ACGT 1 cut(s) 2507
HspAI GCGC 1 cut(s) 20
Ksp22I TGATCA 4 cut(s) 130, 658, 861, 973
LguI GCTCTTC 1 cut(s) 407
LmnI GCTCC 4 cut(s) 410, 746, 2278, 2744
Lsp1109I GCAGC 1 cut(s) 211
LweI GCATC 8 cut(s) 347, 644, 706, 902, 912, 1386, 1588, 1984
MaeI CTAG 5 cut(s) 1209, 1680, 2240, 2403, 2427
MaeII ACGT 1 cut(s) 2507
MaeIII GTNAC 8 cut(s) 37, 318, 1119, 1359, 1415, 1642, 1780, 2389
MbiI CCGCTC 1 cut(s) 405
MfeI CAATTG 3 cut(s) 188, 570, 626
MflI RGATCY 4 cut(s) 276, 707, 1149, 2174
MhlI GDGCHC 3 cut(s) 126, 583, 2543
MlyI GAGTC 3 cut(s) 1726, 2198, 2386
MmeI TCCRAC 1 cut(s) 1944
Mph1103I ATGCAT 1 cut(s) 927
MroXI GAANNNNTTC 1 cut(s) 2618
MslI CAYNNNNRTG 5 cut(s) 1143, 1278, 1838, 2559, 2718
MspA1I CMGCKG 1 cut(s) 2610
MspCI CTTAAG 3 cut(s) 350, 1405, 2774
MspR9I CCNGG 1 cut(s) 1228
MunI CAATTG 3 cut(s) 188, 570, 626
Mva1269I GAATGC 2 cut(s) 1610, 2769
MvaI CCWGG 1 cut(s) 1228
MwoI GCNNNNNNNGC 3 cut(s) 107, 566, 2279
NcoI CCATGG 1 cut(s) 84
NdeI CATATG 1 cut(s) 2258
NlaIV GGNNCC 6 cut(s) 278, 666, 1024, 1727, 1938, 2176
NmuCI GTSAC 4 cut(s) 37, 318, 1780, 2389
NsiI ATGCAT 1 cut(s) 927
NspI RCATGY 2 cut(s) 1483, 2564
NspV TTCGAA 2 cut(s) 1168, 1175
PciSI GCTCTTC 1 cut(s) 407
PctI GAATGC 2 cut(s) 1610, 2769
PdmI GAANNNNTTC 1 cut(s) 2618
PflMI CCANNNNNTGG 1 cut(s) 2077
PkrI GCNGC 3 cut(s) 201, 404, 823
PleI GAGTC 3 cut(s) 1726, 2197, 2386
PpsI GAGTC 3 cut(s) 1726, 2197, 2386
PpuMI RGGWCCY 2 cut(s) 665, 1725
PsiI TTATAA 2 cut(s) 284, 2757
Psp1406I AACGTT 1 cut(s) 2507
Psp5II RGGWCCY 2 cut(s) 665, 1725
Psp6I CCWGG 1 cut(s) 1226
PspEI GGTNACC 2 cut(s) 37, 1642
PspFI CCCAGC 1 cut(s) 47
PspGI CCWGG 1 cut(s) 1226
PspN4I GGNNCC 6 cut(s) 278, 666, 1024, 1727, 1938, 2176
PspPI GGNCC 6 cut(s) 665, 1023, 1541, 1725, 1892, 2267
PspPPI RGGWCCY 2 cut(s) 665, 1725
PstI CTGCAG 1 cut(s) 2516
PsuI RGATCY 4 cut(s) 276, 707, 1149, 2174
RsaI GTAC 3 cut(s) 869, 1478, 1808
RsaNI GTAC 3 cut(s) 868, 1477, 1807
RseI CAYNNNNRTG 5 cut(s) 1143, 1278, 1838, 2559, 2718
SapI GCTCTTC 1 cut(s) 407
SatI GCNGC 3 cut(s) 200, 403, 822
Sau96I GGNCC 6 cut(s) 665, 1023, 1541, 1725, 1892, 2267
SchI GAGTC 3 cut(s) 1726, 2198, 2386
ScrFI CCNGG 1 cut(s) 1228
SduI GDGCHC 3 cut(s) 126, 583, 2543
SfaNI GCATC 8 cut(s) 347, 644, 706, 902, 912, 1386, 1588, 1984
SfcI CTRYAG 2 cut(s) 1802, 2512
SfuI TTCGAA 2 cut(s) 1168, 1175
SinI GGWCC 6 cut(s) 665, 1023, 1541, 1725, 1892, 2267
SmiMI CAYNNNNRTG 5 cut(s) 1143, 1278, 1838, 2559, 2718
SmlI CTYRAG 8 cut(s) 208, 350, 703, 1322, 1352, 1405, 2045, 2774
SmoI CTYRAG 8 cut(s) 208, 350, 703, 1322, 1352, 1405, 2045, 2774
SsiI CCGC 4 cut(s) 403, 822, 2610, 2742
SspI AATATT 3 cut(s) 1302, 1423, 2066
SspMI CTAG 5 cut(s) 1209, 1680, 2240, 2403, 2427
StyD4I CCNGG 1 cut(s) 1226
StyI CCWWGG 3 cut(s) 84, 959, 1981
TaaI ACNGT 7 cut(s) 899, 1476, 1579, 1780, 1806, 2363, 2440
TaiI ACGT 1 cut(s) 2510
TatI WGTACW 1 cut(s) 1476
TauI GCSGC 2 cut(s) 405, 824
TscAI CASTG 7 cut(s) 1033, 1188, 1785, 1800, 1938, 2366, 2402
TseFI GTSAC 4 cut(s) 37, 318, 1780, 2389
TseI GCWGC 1 cut(s) 199
Tsp45I GTSAC 4 cut(s) 37, 318, 1780, 2389
TspGWI ACGGA 3 cut(s) 239, 278, 819
TspRI CASTG 7 cut(s) 1033, 1188, 1785, 1800, 1938, 2366, 2402
Van91I CCANNNNNTGG 1 cut(s) 2077
Vha464I CTTAAG 3 cut(s) 350, 1405, 2774
VpaK11BI GGWCC 6 cut(s) 665, 1023, 1541, 1725, 1892, 2267
XapI RAATTY 7 cut(s) 330, 853, 1212, 1343, 1378, 1512, 2101
XbaI TCTAGA 3 cut(s) 1208, 1679, 2239
XceI RCATGY 2 cut(s) 1483, 2564
XcmI CCANNNNNNNNNTGG 3 cut(s) 388, 889, 2103
XmiI GTMKAC 2 cut(s) 439, 2675
XmnI GAANNNNTTC 1 cut(s) 2618
XspI CTAG 5 cut(s) 1209, 1680, 2240, 2403, 2427
Zsp2I ATGCAT 1 cut(s) 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.