Rroxscaffold_3G00246470

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000085
Physical Location & Seq
Forward (+)
39584895 .. 39589879
4985 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00246470.1

Sequence Viewer

Length: 3561 bp
ATGGATCAGCTTATGAAGGCATTAAAAGATAACAACGTCACTGCCGTTGGGGTGTACGGCATGGGGGGCGTTGGAAAGACGACCCTTGTGAAACATGTCAGTCAAGAAGCTCGGAAGCAAGGCCTTTTTGATCATGTCATTATGGTTAGAGTTAATCAAAACCCTGTTTTGAAAAATACTCAAGGCATGTTGGCAGATGGGCTAGGCATCCAGTTGACGAACGAGACAGCGGAGGGAAGAGCTTCAAGATTGTCTTCGGAGATAAAGAGCAGAAAGAAAATCCTTATAATCTTGGACAACGTTTGGGCTAAAATAGAGCTGTCAGACATAGGAATTCCCAACAATGACAGTCTTCAAATTTGCAACTCCAAAGTCCTACTTACCACAAGGATAGGGAATGCTTGTCATGTCATGGACAGCCAAGAAATCACCCTCAATAGCCTATCGAAAGAAGATTCATGGACATTGTTTGTGAAATATGCAAAGAAGTCTTTTGAATCGAGCGATTTCGAGGATGTCGCAAGGAAGGTAGCTCGAGAATGTGGTGGTCTACCAATTGCATTGACAGCAGTTGCAAGGGCACTTGGAGATAAAGACCAGGAAGAATGGGAACGCGCAGCTGAACGGCTAGAGGAGTCGCAAGCTGTCAACCATGACGATGAACGACGTGCATACGAATGCATAAAATTCAGCTATGATTTCTTGAGAAATGAGGACTTAAAGTCATTCTTCTTGCTTTGTTGCCTATTCCCAGAAGGCTATGACATCCAAATAGAAGACTTGTTCAAGTATGGGATTGGGACAGGATTGTTTAAAGATGCTAGGACAATCCAAATGGCCAGAGGAGAAGCTGGTTCAGCAGCCAAGCACCTTACACGTTCTAGCTTGCTTTTGGATAGTGAAATCCATGGAAGTGTTAGGATGCATGATGTCATCCGGGATACAGCCATTCAAATTGCACGATCTGAAGGTGAGCGCAGATTTTGCGTAGAAGCTGGCTGTCGTTTGGAGAATTGGCCACTTCAATTGCATGAAGACCACTCTGCAATTTCACTCATGAAGAACGACATTTGCGAGCTGCCTGAAGAGCTGGTATGTCCGAAACTCCAAATTTTATTGCTACAAGAGAATCATGATTTAGATCAGATCCCTGATACTTTTTTTGAAACTACTAGTTCACTAAGAGTCGTAGATCTTAGTTACACTAGTATATCATCCCTATCCAAGTCATTTGAATTCCTAAGCAATCTCAAAGGTTTGTATTTGGATTATTGCCGGAACATAGCTGACATTTCCATACTGAAATATTTTAAGAAGCTTGAGATTCTCAGTATGAAAGAATACCCTCTAAAGGAATTGTCAACAGAAATTGGAAATTTGACCAATTTAAGGGTGTTGGATGTTAGTGGCGAGGGTCCAGGCGGTGGAGGTATCATCAGAATTCCATCCGGGGTGATATCAAAATTGCCTAGGTCAGAAGAAATATGCCTGCACTGTGGATTTTGGGACTGGGGGAGTAAAGTTGAAGGAGAAGGAGAAACAACTAATGTTAGCTTTGATGAGGTAACTAGCTTATCATATTTGAACAGTTTGAAGGTTTGCATATCTGATGTGAATTGCATCCCTAGTTATGTTAAAAAGGATGGGAAGAAGCTGAGTTGGACTTGCTTTGATATATGTATTGGTAGAGACAGGAACGTCAGAACTAATATCAGAATTGGTAGAGACAGGGACGTCGAAAATATTATCAGAGACCTTGGTAGATGTATTGATAGAGAGAGGAACACCAGAAATATTATCAGAGACCTGCGCATTCAATTCAAGCGAGATGATCATAAATATTCAAGATCTTTGATTCTTGATACAACCATTAGTATGTTACCAGCTTGGTTTGTCACGGCGGTGACAGAGAAGACAGAGAGGCTAGTGTATGTGAATTTCACAGGGTTAAATAACATCTTCATGGAATATCACTATGGGAGGTTACATAGTCTGAAGCATCTCTCTGTTATTGGTCCCGATGAGAGCTTGGAAGCATTGATCCTTGTGGACATAACAGGAAGGAATCAAAATAAAGCTGTGTTCGAGAAATTGGAAGAGTTGCATCTGGGCGGTCTGGATTCCTTGAAGGAGTTATGTTCTGGTGAATTACCATCTAGGTCTCTCTGGAATCTGAAAGTATTGGAGGTACGTGATTGTCCTGACTTGGAAAATGTCCTGTTACCATCAAAATTGTTACAGAGACTGGCAAATCTGGAGAACCTATTTTGTAAGTGTATGCCTGGAATGGAATATGTGTTTGGATGTGAAGGATTTGAGCTAGTAGAATCAAAACTGAAAAAGATGGAGTTGTTTCAGCTAGATGCAGTAAAGAGCATCTGTATTGGTCCTGCTCCACCTGCAATGTTCCAAGCACTTCAGAGTTTGTCCATTTACGATTGCAACTTTCTAGGAGGTCTCTTCACATTTGATGTAGCTCAATGTCTTTTTCAAGTGGAAATCATTTCGGTAGTGAGTTGCCGTAACTTGGAAAGAGTAATCGAAGCAAGCAAGGAATCAGAGCTCAGAAAGAAGACCGTTCTTCCAAAATTGAAGTCCCTAGTCTTGGTGAATCTTCCTATGTTGTACAGTGGTGGTGCTGCTATTGATATGGAGTGTCCTTCATTGCAACACTTGTATGTGAGAGACTGCCCTCATTTTTCAGCCTCTGCTGATGACTTCGGGAGCAATAACGAAGTCGTACTCAACGATGTACTGCATTACTTTACTCAAACCGCAAGACTCCAAAAACCAGGGGATAATTCCGACTCATATCTCCAGGCCGAGGAAAAGATCCGTACATTGATGGAGAATCAATTGGAGCCGACTGTGCCTGTCTTGAGAAAGGGGGTTGCTGACTTCCTGTATAACTCACCTCAAGCCAAGCAGATTACGCCCAAATATGAGGACGGTAACTGGTTAGAGCACAGGATGAAGTGGGATGAATTGGTCAGGAAGACGCCCGGAGCGATGGAGTATATAATTGGATCGTACGCGAAGATAGAACCTGAAGCGTTGAATCACGTCCCTGAATTAATGGATAAGATGCAATCGATGGAGGGACTGCAGCGACTGCTGGACGACAATCCGCCTGAGGATAGGCCAGACGACCTATTCGCTGATAAGACGTTATTTCCCGAGTTTATAAGGGTAATAGAACGTTTAGTTGAGATTAAATATGGAAGTGCACTTGACGGTGAATCTGAGAGTGCATTTGACATTGACAGTGAATCTGACAGGGCACTTCTGAGTGAATTAATCAGTGTAGCGTTAAGTGCATCTCAGAGTTCATCCTTCCGAGACAGTCAATCTGAGAGTGCATCGTCAAGTGTATTTATCAGTGCATTGTCACGTGCATTTCAGATGGTCAAGTGCGCTGAAGAGTTCTTCCCTCGACACAGTGAATCTGAGAGTGCATTTCAGAGTTCATCCATCCGAGACATTGAATCTGAGATTGCATTTGACAGTGAATTTATCAGTGGATTGAAAAGTGCATTTCAGAGTTCATCCAGCAAGAAATGA

Protein Analysis

1186

Amino Acids

133.69

Weight (kDa)

5.2

Isoelectric Point (pI)

45.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 1 - 162 3.7e-26 NB-ARC domain
LRR_8 PF13855 367 - 426 6.3e-10 Leucine rich repeat
LRR_14 PF23598 391 - 469 1.2e-06 Leucine-rich repeat region
LRR_RPS2 PF23247 698 - 841 2.1e-19 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 780 - 900 4.1e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 287, 3185
AarI CACCTGC 1 cut(s) 2407
AasI GACNNNNNNGTC 2 cut(s) 1697, 1733
AatII GACGTC 1 cut(s) 1737
Acc16I TGCGCA 1 cut(s) 1811
Acc36I ACCTGC 2 cut(s) 1815, 2407
AccB7I CCANNNNNTGG 1 cut(s) 1424
AccI GTMKAC 1 cut(s) 550
AccII CGCG 2 cut(s) 615, 3035
AciI CCGC 6 cut(s) 230, 1422, 1901, 2112, 2775, 3128
AclI AACGTT 2 cut(s) 300, 3199
AclWI GGATC 5 cut(s) 12, 1141, 2035, 2827, 3034
AcoI YGGCCR 2 cut(s) 837, 1016
AcsI RAATTY 9 cut(s) 333, 357, 686, 1110, 1235, 1375, 1440, 1936, 3509
AcuI CTGAAG 6 cut(s) 987, 1104, 2015, 2402, 3069, 3438
AcvI CACGTG 1 cut(s) 3392
AcyI GRCGYC 2 cut(s) 1734, 2999
AfaI GTAC 7 cut(s) 56, 2190, 2627, 2742, 2754, 2839, 3032
AfiI CCNNNNNNNGG 6 cut(s) 1351, 1389, 1424, 2527, 2605, 2824
AflIII ACRYGT 2 cut(s) 94, 875
AhdI GACNNNNNGTC 1 cut(s) 721
AhlI ACTAGT 2 cut(s) 1172, 1205
AjiI CACGTC 2 cut(s) 668, 3064
AjnI CCWGG 5 cut(s) 597, 1417, 2281, 2791, 2817
AjuI GAANNNNNNNTTGG 2 cut(s) 2283, 2315
AleI CACNNNNGTG 1 cut(s) 1901
Alw21I GWGCWC 3 cut(s) 2565, 2967, 3229
Alw44I GTGCAC 1 cut(s) 3225
AlwI GGATC 5 cut(s) 12, 1141, 2035, 2827, 3034
AlwNI CAGNNNCTG 3 cut(s) 2245, 2708, 3112
Ama87I CYCGRG 2 cut(s) 534, 3176
AoxI GGCC 5 cut(s) 121, 837, 1016, 2820, 3140
ApaLI GTGCAC 1 cut(s) 3225
ApeKI GCWGC 5 cut(s) 617, 860, 1078, 2639, 3106
ApoI RAATTY 9 cut(s) 333, 357, 686, 1110, 1235, 1375, 1440, 1936, 3509
ArsI GACNNNNNNTTYG 4 cut(s) 2579, 2611, 3137, 3169
AseI ATTAAT 2 cut(s) 3074, 3296
Asp700I GAANNNNTTC 1 cut(s) 241
AspA2I CCTAGG 1 cut(s) 1469
AspLEI GCGC 4 cut(s) 617, 978, 1812, 3416
AspS9I GGNCC 3 cut(s) 1415, 2015, 2387
AsuC2I CCSGG 3 cut(s) 938, 1450, 3003
AsuHPI GGTGA 8 cut(s) 421, 983, 1465, 1915, 2156, 2620, 2904, 3248
AvaI CYCGRG 2 cut(s) 534, 3176
AvaII GGWCC 3 cut(s) 1415, 2015, 2387
AvrII CCTAGG 1 cut(s) 1469
AxyI CCTNAGG 1 cut(s) 3132
BaeGI GKGCMC 3 cut(s) 583, 3229, 3283
BalI TGGCCA 2 cut(s) 839, 1018
BanII GRGCYC 1 cut(s) 2565
BbrPI CACGTG 1 cut(s) 3392
BbsI GAAGAC 7 cut(s) 246, 344, 783, 1041, 1919, 2579, 3002
Bbv12I GWGCWC 3 cut(s) 2565, 2967, 3229
BbvI GCAGC 5 cut(s) 629, 872, 1065, 2626, 3118
BceAI ACGGC 5 cut(s) 29, 73, 641, 1914, 2505
BciT130I CCWGG 5 cut(s) 599, 1419, 2283, 2793, 2819
BciVI GTATCC 1 cut(s) 934
BclI TGATCA 2 cut(s) 130, 1831
BcnI CCSGG 3 cut(s) 938, 1450, 3003
BcuI ACTAGT 2 cut(s) 1172, 1205
BfmI CTRYAG 1 cut(s) 3104
BfuAI ACCTGC 2 cut(s) 1815, 2407
BfuI GTATCC 1 cut(s) 934
BglII AGATCT 2 cut(s) 1192, 1847
BisI GCNGC 5 cut(s) 618, 861, 1079, 2640, 3107
BlnI CCTAGG 1 cut(s) 1469
BlsI GCNGC 5 cut(s) 619, 862, 1080, 2641, 3108
Bme1390I CCNGG 8 cut(s) 599, 938, 1419, 1450, 2283, 2793, 2819, 3003
Bme18I GGWCC 3 cut(s) 1415, 2015, 2387
BmeRI GACNNNNNGTC 1 cut(s) 721
BmeT110I CYCGRG 2 cut(s) 534, 3176
BmgBI CACGTC 2 cut(s) 668, 3064
BmgT120I GGNCC 3 cut(s) 1415, 2015, 2387
BmiI GGNNCC 3 cut(s) 1416, 2017, 2862
BmrFI CCNGG 8 cut(s) 599, 938, 1419, 1450, 2283, 2793, 2819, 3003
BmrI ACTGGG 1 cut(s) 1519
BmuI ACTGGG 1 cut(s) 1519
BpiI GAAGAC 7 cut(s) 246, 344, 783, 1041, 1919, 2579, 3002
BpmI CTGGAG 2 cut(s) 2276, 2801
Bpu10I CCTNAGC 1 cut(s) 1241
BpuEI CTTGAG 5 cut(s) 165, 724, 1340, 2899, 2901
BpuMI CCSGG 3 cut(s) 938, 1450, 3003
Bsa29I ATCGAT 1 cut(s) 3092
BsaAI YACGTR 2 cut(s) 2192, 3392
BsaBI GATNNNNATC 1 cut(s) 1140
BsaHI GRCGYC 2 cut(s) 1734, 2999
BsaI GGTCTC 4 cut(s) 1746, 1797, 2166, 2462
BsaJI CCNNGG 6 cut(s) 907, 1449, 1469, 1756, 2792, 2823
Bsc4I CCNNNNNNNGG 6 cut(s) 1351, 1389, 1424, 2527, 2605, 2824
Bse1I ACTGG 4 cut(s) 211, 1514, 2250, 2960
Bse21I CCTNAGG 1 cut(s) 3132
Bse3DI GCAATG 2 cut(s) 2409, 2663
Bse8I GATNNNNATC 1 cut(s) 1140
BseBI CCWGG 5 cut(s) 599, 1419, 2283, 2793, 2819
BseCI ATCGAT 1 cut(s) 3092
BseDI CCNNGG 6 cut(s) 907, 1449, 1469, 1756, 2792, 2823
BseJI GATNNNNATC 1 cut(s) 1140
BseLI CCNNNNNNNGG 6 cut(s) 1351, 1389, 1424, 2527, 2605, 2824
BseMI GCAATG 2 cut(s) 2409, 2663
BseNI ACTGG 4 cut(s) 211, 1514, 2250, 2960
BseRI GAGGAG 2 cut(s) 647, 858
BseSI GKGCMC 3 cut(s) 583, 3229, 3283
BseXI GCAGC 5 cut(s) 629, 872, 1065, 2626, 3118
BsgI GTGCAG 1 cut(s) 1475
Bsh1236I CGCG 2 cut(s) 615, 3035
BshFI GGCC 5 cut(s) 123, 839, 1018, 2822, 3142
BshVI ATCGAT 1 cut(s) 3092
BsiHKAI GWGCWC 3 cut(s) 2565, 2967, 3229
BsiHKCI CYCGRG 2 cut(s) 534, 3176
BsiSI CCGG 4 cut(s) 937, 1276, 1449, 3003
BsiWI CGTACG 1 cut(s) 3030
BslFI GGGAC 7 cut(s) 814, 1520, 1745, 2001, 2581, 3050, 3114
BslI CCNNNNNNNGG 6 cut(s) 1351, 1389, 1424, 2527, 2605, 2824
BsmFI GGGAC 7 cut(s) 814, 1520, 1745, 2001, 2581, 3050, 3114
BsmI GAATGC 3 cut(s) 403, 683, 1812
BsnI GGCC 5 cut(s) 123, 839, 1018, 2822, 3142
Bso31I GGTCTC 4 cut(s) 1746, 1797, 2166, 2462
BsoBI CYCGRG 2 cut(s) 534, 3176
Bsp1286I GDGCHC 5 cut(s) 583, 2565, 2967, 3229, 3283
Bsp1407I TGTACA 1 cut(s) 2625
Bsp19I CCATGG 1 cut(s) 907
BspACI CCGC 6 cut(s) 230, 1422, 1901, 2112, 2775, 3128
BspANI GGCC 5 cut(s) 123, 839, 1018, 2822, 3142
BspDI ATCGAT 1 cut(s) 3092
BspFNI CGCG 2 cut(s) 615, 3035
BspHI TCATGA 2 cut(s) 1056, 1132
BspLI GGNNCC 3 cut(s) 1416, 2017, 2862
BspMAI CTGCAG 1 cut(s) 3108
BspMI ACCTGC 2 cut(s) 1815, 2407
BspPI GGATC 5 cut(s) 12, 1141, 2035, 2827, 3034
BspQI GCTCTTC 2 cut(s) 232, 1080
BspTNI GGTCTC 4 cut(s) 1746, 1797, 2166, 2462
BsrDI GCAATG 2 cut(s) 2409, 2663
BsrGI TGTACA 1 cut(s) 2625
BsrI ACTGG 4 cut(s) 211, 1514, 2250, 2960
BssECI CCNNGG 6 cut(s) 907, 1449, 1469, 1756, 2792, 2823
BssNI GRCGYC 2 cut(s) 1734, 2999
BssT1I CCWWGG 3 cut(s) 907, 1469, 1756
Bst2UI CCWGG 5 cut(s) 599, 1419, 2283, 2793, 2819
Bst6I CTCTTC 5 cut(s) 232, 1080, 2091, 2465, 3414
BstACI GRCGYC 2 cut(s) 1734, 2999
BstAPI GCANNNNNTGC 2 cut(s) 984, 3112
BstAUI TGTACA 1 cut(s) 2625
BstBAI YACGTR 2 cut(s) 2192, 3392
BstC8I GCNNGC 6 cut(s) 642, 887, 997, 1076, 1490, 2548
BstDSI CCRYGG 1 cut(s) 907
BstFNI CGCG 2 cut(s) 615, 3035
BstHHI GCGC 4 cut(s) 617, 978, 1812, 3416
BstNI CCWGG 5 cut(s) 599, 1419, 2283, 2793, 2819
BstNSI RCATGY 2 cut(s) 98, 190
BstSCI CCNGG 8 cut(s) 597, 936, 1417, 1448, 2281, 2791, 2817, 3001
BstSFI CTRYAG 1 cut(s) 3104
BstSLI GKGCMC 3 cut(s) 583, 3229, 3283
BstUI CGCG 2 cut(s) 615, 3035
BstV1I GCAGC 5 cut(s) 629, 872, 1065, 2626, 3118
BstV2I GAAGAC 7 cut(s) 246, 344, 783, 1041, 1919, 2579, 3002
BstX2I RGATCY 4 cut(s) 1146, 1192, 1847, 2832
BstYI RGATCY 4 cut(s) 1146, 1192, 1847, 2832
Bsu15I ATCGAT 1 cut(s) 3092
Bsu36I CCTNAGG 1 cut(s) 3132
BsuI GTATCC 1 cut(s) 934
BsuRI GGCC 5 cut(s) 123, 839, 1018, 2822, 3142
BsuTUI ATCGAT 1 cut(s) 3092
BtgI CCRYGG 1 cut(s) 907
BtgZI GCGATG 1 cut(s) 3023
BtrI CACGTC 2 cut(s) 668, 3064
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 9 cut(s) 39, 1492, 2635, 3271, 3307, 3385, 3445, 3511, 3523
BveI ACCTGC 2 cut(s) 1815, 2407
Cac8I GCNNGC 6 cut(s) 642, 887, 997, 1076, 1490, 2548
CaiI CAGNNNCTG 3 cut(s) 2245, 2708, 3112
CciI TCATGA 2 cut(s) 1056, 1132
CfoI GCGC 4 cut(s) 617, 978, 1812, 3416
Cfr13I GGNCC 3 cut(s) 1415, 2015, 2387
ClaI ATCGAT 1 cut(s) 3092
CseI GACGC 1 cut(s) 3007
Csp6I GTAC 7 cut(s) 55, 2189, 2626, 2741, 2753, 2838, 3031
CspCI CAANNNNNGTGG 2 cut(s) 1008, 1043
CviQI GTAC 7 cut(s) 55, 2189, 2626, 2741, 2753, 2838, 3031
DraI TTTAAA 1 cut(s) 814
DrdI GACNNNNNNGTC 2 cut(s) 1697, 1733
DriI GACNNNNNGTC 1 cut(s) 721
DseDI GACNNNNNNGTC 2 cut(s) 1697, 1733
EaeI YGGCCR 2 cut(s) 837, 1016
Eam1104I CTCTTC 5 cut(s) 232, 1080, 2091, 2465, 3414
Eam1105I GACNNNNNGTC 1 cut(s) 721
EarI CTCTTC 5 cut(s) 232, 1080, 2091, 2465, 3414
EciI GGCGGA 1 cut(s) 3117
Ecl136II GAGCTC 1 cut(s) 2563
Eco130I CCWWGG 3 cut(s) 907, 1469, 1756
Eco147I AGGCCT 1 cut(s) 123
Eco24I GRGCYC 1 cut(s) 2565
Eco31I GGTCTC 4 cut(s) 1746, 1797, 2166, 2462
Eco32I GATATC 1 cut(s) 1458
Eco47I GGWCC 3 cut(s) 1415, 2015, 2387
Eco53kI GAGCTC 1 cut(s) 2563
Eco57I CTGAAG 6 cut(s) 987, 1104, 2015, 2402, 3069, 3438
Eco72I CACGTG 1 cut(s) 3392
Eco81I CCTNAGG 1 cut(s) 3132
Eco88I CYCGRG 2 cut(s) 534, 3176
EcoICRI GAGCTC 1 cut(s) 2563
EcoRI GAATTC 3 cut(s) 333, 1235, 1440
EcoRII CCWGG 5 cut(s) 597, 1417, 2281, 2791, 2817
EcoRV GATATC 1 cut(s) 1458
EcoT14I CCWWGG 3 cut(s) 907, 1469, 1756
EcoT22I ATGCAT 2 cut(s) 683, 927
EcoT38I GRGCYC 1 cut(s) 2565
ErhI CCWWGG 3 cut(s) 907, 1469, 1756
FalI AAGNNNNNCTT 6 cut(s) 238, 270, 363, 395, 713, 745
FaqI GGGAC 7 cut(s) 814, 1520, 1745, 2001, 2581, 3050, 3114
FbaI TGATCA 2 cut(s) 130, 1831
FblI GTMKAC 1 cut(s) 550
Fnu4HI GCNGC 5 cut(s) 618, 861, 1079, 2640, 3107
FriOI GRGCYC 1 cut(s) 2565
Fsp4HI GCNGC 5 cut(s) 618, 861, 1079, 2640, 3107
FspI TGCGCA 1 cut(s) 1811
GlaI GCGC 4 cut(s) 616, 977, 1811, 3415
GluI GCNGC 5 cut(s) 618, 861, 1079, 2640, 3107
GsuI CTGGAG 2 cut(s) 2276, 2801
HaeIII GGCC 5 cut(s) 123, 839, 1018, 2822, 3142
HapII CCGG 4 cut(s) 937, 1276, 1449, 3003
HgaI GACGC 1 cut(s) 3007
HhaI GCGC 4 cut(s) 617, 978, 1812, 3416
Hin1I GRCGYC 2 cut(s) 1734, 2999
Hin6I GCGC 4 cut(s) 615, 976, 1810, 3414
HinP1I GCGC 4 cut(s) 615, 976, 1810, 3414
HincII GTYRAC 3 cut(s) 216, 649, 1362
HindII GTYRAC 3 cut(s) 216, 649, 1362
HindIII AAGCTT 1 cut(s) 1316
HpaII CCGG 4 cut(s) 937, 1276, 1449, 3003
HphI GGTGA 8 cut(s) 421, 983, 1465, 1915, 2156, 2620, 2904, 3248
Hpy166II GTNNAC 8 cut(s) 55, 216, 551, 649, 1178, 1362, 2050, 3227
Hpy8I GTNNAC 8 cut(s) 55, 216, 551, 649, 1178, 1362, 2050, 3227
Hpy99I CGWCG 2 cut(s) 669, 1739
Hsp92I GRCGYC 2 cut(s) 1734, 2999
HspAI GCGC 4 cut(s) 615, 976, 1810, 3414
Ksp22I TGATCA 2 cut(s) 130, 1831
LguI GCTCTTC 2 cut(s) 232, 1080
LmnI GCTCC 4 cut(s) 2398, 2724, 2860, 3005
Lsp1109I GCAGC 5 cut(s) 629, 872, 1065, 2626, 3118
MfeI CAATTG 3 cut(s) 555, 1025, 2855
MflI RGATCY 4 cut(s) 1146, 1192, 1847, 2832
MhlI GDGCHC 5 cut(s) 583, 2565, 2967, 3229, 3283
MlsI TGGCCA 2 cut(s) 839, 1018
MluNI TGGCCA 2 cut(s) 839, 1018
MlyI GAGTC 4 cut(s) 644, 1194, 2775, 2801
MmeI TCCRAC 4 cut(s) 52, 1377, 1640, 2829
Mox20I TGGCCA 2 cut(s) 839, 1018
Mph1103I ATGCAT 2 cut(s) 683, 927
MroXI GAANNNNTTC 1 cut(s) 241
MscI TGGCCA 2 cut(s) 839, 1018
MslI CAYNNNNRTG 7 cut(s) 657, 676, 912, 1874, 1901, 1961, 2676
Msp20I TGGCCA 2 cut(s) 839, 1018
MspA1I CMGCKG 2 cut(s) 230, 620
MspI CCGG 4 cut(s) 937, 1276, 1449, 3003
MspR9I CCNGG 8 cut(s) 599, 938, 1419, 1450, 2283, 2793, 2819, 3003
MunI CAATTG 3 cut(s) 555, 1025, 2855
Mva1269I GAATGC 3 cut(s) 403, 683, 1812
MvaI CCWGG 5 cut(s) 599, 1419, 2283, 2793, 2819
MvnI CGCG 2 cut(s) 615, 3035
NciI CCSGG 3 cut(s) 938, 1450, 3003
NcoI CCATGG 1 cut(s) 907
NlaIV GGNNCC 3 cut(s) 1416, 2017, 2862
NmeAIII GCCGAG 1 cut(s) 2848
NmuCI GTSAC 4 cut(s) 37, 1894, 1903, 3387
NsbI TGCGCA 1 cut(s) 1811
NsiI ATGCAT 2 cut(s) 683, 927
NspI RCATGY 2 cut(s) 98, 190
OliI CACNNNNGTG 1 cut(s) 1901
PaeR7I CTCGAG 1 cut(s) 534
PagI TCATGA 2 cut(s) 1056, 1132
PaqCI CACCTGC 1 cut(s) 2407
PceI AGGCCT 1 cut(s) 123
PciI ACATGT 1 cut(s) 94
PciSI GCTCTTC 2 cut(s) 232, 1080
PcsI WCGNNNNNNNCGW 4 cut(s) 42, 1071, 2745, 3005
PctI GAATGC 3 cut(s) 403, 683, 1812
PdmI GAANNNNTTC 1 cut(s) 241
Pfl23II CGTACG 1 cut(s) 3030
PflMI CCANNNNNTGG 1 cut(s) 1424
PfoI TCCNGGA 1 cut(s) 936
PkrI GCNGC 5 cut(s) 619, 862, 1080, 2641, 3108
PleI GAGTC 4 cut(s) 643, 1193, 2775, 2801
PmaCI CACGTG 1 cut(s) 3392
PmlI CACGTG 1 cut(s) 3392
PpsI GAGTC 4 cut(s) 643, 1193, 2775, 2801
Ppu21I YACGTR 2 cut(s) 2192, 3392
PscI ACATGT 1 cut(s) 94
PshBI ATTAAT 2 cut(s) 3074, 3296
PsiI TTATAA 2 cut(s) 287, 3185
Psp124BI GAGCTC 1 cut(s) 2565
Psp1406I AACGTT 2 cut(s) 300, 3199
Psp6I CCWGG 5 cut(s) 597, 1417, 2281, 2791, 2817
PspCI CACGTG 1 cut(s) 3392
PspGI CCWGG 5 cut(s) 597, 1417, 2281, 2791, 2817
PspLI CGTACG 1 cut(s) 3030
PspN4I GGNNCC 3 cut(s) 1416, 2017, 2862
PspPI GGNCC 3 cut(s) 1415, 2015, 2387
PstI CTGCAG 1 cut(s) 3108
PstNI CAGNNNCTG 3 cut(s) 2245, 2708, 3112
PsuI RGATCY 4 cut(s) 1146, 1192, 1847, 2832
PvuII CAGCTG 1 cut(s) 620
RsaI GTAC 7 cut(s) 56, 2190, 2627, 2742, 2754, 2839, 3032
RsaNI GTAC 7 cut(s) 55, 2189, 2626, 2741, 2753, 2838, 3031
RseI CAYNNNNRTG 7 cut(s) 657, 676, 912, 1874, 1901, 1961, 2676
SacI GAGCTC 1 cut(s) 2565
SapI GCTCTTC 2 cut(s) 232, 1080
SatI GCNGC 5 cut(s) 618, 861, 1079, 2640, 3107
Sau96I GGNCC 3 cut(s) 1415, 2015, 2387
SchI GAGTC 4 cut(s) 644, 1194, 2775, 2801
ScrFI CCNGG 8 cut(s) 599, 938, 1419, 1450, 2283, 2793, 2819, 3003
SduI GDGCHC 5 cut(s) 583, 2565, 2967, 3229, 3283
SfcI CTRYAG 1 cut(s) 3104
Sfr274I CTCGAG 1 cut(s) 534
SinI GGWCC 3 cut(s) 1415, 2015, 2387
SlaI CTCGAG 1 cut(s) 534
SmiMI CAYNNNNRTG 7 cut(s) 657, 676, 912, 1874, 1901, 1961, 2676
SmlI CTYRAG 6 cut(s) 180, 534, 703, 1319, 2878, 2916
SmoI CTYRAG 6 cut(s) 180, 534, 703, 1319, 2878, 2916
SpeI ACTAGT 2 cut(s) 1172, 1205
SseBI AGGCCT 1 cut(s) 123
SsiI CCGC 6 cut(s) 230, 1422, 1901, 2112, 2775, 3128
SspI AATATT 4 cut(s) 1307, 1744, 1795, 1841
SstI GAGCTC 1 cut(s) 2565
StuI AGGCCT 1 cut(s) 123
StyD4I CCNGG 8 cut(s) 597, 936, 1417, 1448, 2281, 2791, 2817, 3001
StyI CCWWGG 3 cut(s) 907, 1469, 1756
TaqI TCGA 9 cut(s) 446, 500, 510, 535, 1737, 2085, 2541, 3092, 3433
TatI WGTACW 2 cut(s) 2625, 2752
TscAI CASTG 9 cut(s) 46, 1499, 2635, 3271, 3307, 3385, 3445, 3511, 3523
TseFI GTSAC 4 cut(s) 37, 1894, 1903, 3387
TseI GCWGC 5 cut(s) 617, 860, 1078, 2639, 3106
Tsp45I GTSAC 4 cut(s) 37, 1894, 1903, 3387
TspGWI ACGGA 1 cut(s) 2825
TspRI CASTG 9 cut(s) 46, 1499, 2635, 3271, 3307, 3385, 3445, 3511, 3523
Van91I CCANNNNNTGG 1 cut(s) 1424
VneI GTGCAC 1 cut(s) 3225
VpaK11BI GGWCC 3 cut(s) 1415, 2015, 2387
VspI ATTAAT 2 cut(s) 3074, 3296
XapI RAATTY 9 cut(s) 333, 357, 686, 1110, 1235, 1375, 1440, 1936, 3509
XceI RCATGY 2 cut(s) 98, 190
XhoI CTCGAG 1 cut(s) 534
XmaJI CCTAGG 1 cut(s) 1469
XmiI GTMKAC 1 cut(s) 550
XmnI GAANNNNTTC 1 cut(s) 241
ZraI GACGTC 1 cut(s) 1735
Zsp2I ATGCAT 2 cut(s) 683, 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.