RchiOBHm_Chr5g0080371

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000085
Physical Location & Seq
Reverse (-)
86327388 .. 86338147
10760 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35468

Sequence Viewer

Length: 4725 bp
ATGGCCTCGATCCCTAAAAAAACTGATGCTTCTCTTCCTTCATCAGCTGAACCATCATCAACAGCTCGTCGCTGGAAGTATGACGTGTTTTTGAGTTTCAGGGGTACTGACTTTCTAAGGGGTTTTCCGTCCCATTTATACTATCAGCTGCAAGAGAGGGGAATCACAACATTCATGGATGACTCAGATCTTGAAGCAGCGGATGATATGTCTCCCACTCTCCTAGAGGCAATTGAAGGATCAAGGCTCGCAATTGTTGTTCTGACTTCAAATTATGCTTCTTCTGCTCGGTGTTTGGAGCAACTTACAAAGATTTGTCAGATCATGGAGGACGGCACCAGAATTCTTCCAGTTTATTATGTCGACCCTATTGATGTAAGAAATCAACGGGGGCAATTTCAAGATGCTTTCGCCATTTATGAAAAGTATGGGCAATACACGTCGGAGAAGGTCCAGCAGTGGAGAGATGCCTTAAGAAAAGTGGGCAGTTTGCCTGGGTTGAATACACGGGATTACAGGACTCACAGAGATTTTGTCGAAGCCATTTTGGAATCCGTGTGCTACAAAGTACAATCTGGTGTGGAAATCGAAAAAGTACAACCTATTGAAATCAATACGAGAGGTCCTGAAGTAATTGAAGAAACAACACAAGCCATGGATAGGCTTACAGATGATGATGAGGTCAGTGCCGTTGGGGTCTACGGGATGGGAGGCGGAGGCAAGATAACCCTGAAAAACCCGTTGGAGTCTTCTTCATCATCAGCTGTTGAGTGGAAGTATGATGTGTATTTGAGTTTCAGGGGTGTAGATATTGGAAGGGGTTTTACTTCCCATTTATACGATTGTCTAAAAAAGAGAGGAATCAATGCATTCATGGATGAACTAGATCTTCAAGTATGGGATAGTATTTCTGGCGCTTCCATAAAGGCAATTGAAAAATCAAGGTTTGCAATCGTTGTTCTCTCGCAAGACTACGTTTCTTCTACTTGGTGTTTGCATGAACTTACAAAAATCTGTGAGTGCATGGAAGACCAGGGCAGAATTCTTCCAGTTTTTTATAATGTGGAACCCTCTGATGTACGGTACCGGAAGAGGAGTTTTGGAGAAGCTTTCGATAAGCATGAAAGCTCTAGGCGACACACATCAGACAAGGTGAAGCGCTTGATAGATGTTTTAAACCATGTAGCCAGTCTCTTTGGGTGGCATGCAAAGCTTTACAAGACTGATAGAGAACTTGTGGACAATATTGTGGATTTTGTGTGCAGTAAAGTACACCCTATTGAAATTGAGTTTACAATGTCCACCGGAGATTTTGAAGCATTTGAATCAAGAAGAACAGCCATGAATGAGGTTATGAAGGCGCTTACAGATGATAAGGTCACTGCCGTTGGGGTCTACGGAATCGGAGGTGTTGGAAAAACAACCATGGTTGAACATGTTAGTGAAGCAGTTCAGAATAATGGGATGTTCCAGCATGTGATCACGGCTGTTGTATCCAAAATCCCCAACTTGAGAAAAATTCAAGGCACATTGGCAGATATGTTGGGTTTTATGTTCGAGGAGGAGACAGAAATTGGAAGATCCCGTAGATTGATGCAGAATATAAGGAGAGGAAATAGGATCCTTATAATCTTGGACGACATTTGGGAGAGAATAGATTTGTCCAACATTGGAATTCCCAGCTACCACGAGCTTCAAAGATGCAATTCCAAAGTCCTACTCACCACAAGGACACAGAATGTCTGTTGTGCCATGAAGTGCCAGTCAAACATTTCTCTTGATGTCTTATCAGAAGAAGATTCTTGGACCTTATTTGTGAACAAAGCAAGAATGTCTTTTGAATCAACCAATTTTTGTGATGTTGCAAAGAAGGTAGCTAGAGCATGTGCTGGTCTTCCAGTTGCGTTGATAGCAGTTGCGAAGGCACTTGGTGATAAGTGTTTGGATGACTGGACAGAAGCAGCTCGACGACTAGAGGCTTCTCAACCTGCCAACCTTGAAGATGGGAAAGTAGCGTTCAATTGTATAAAATTAAGCTATGATTACTTAATATCTGATGATGCCAGGTCATGCTTTTTGCTTTGTTGCCTATTCCCAGAAGACTATGATATCCAAATTGAAGACTTGCTAAAATATGGGATGGGGAAAGGATTGTTTCAAGATTCCAGCACAATGCATGATGCCAGAGCTAGTGCATATTCAGTGGTCAAGTACCTTAAAGATTGTGGCTTACTTTTGAAATCTGAACGGGACGGATGTGTAAAGATGCATGATGTCATTCGGGATGTGGCCATACGGGTTTCGTCATCCGAGGATGGCCCACTGTTTTTTGTACAAGCTGATTGTCAGTTAAAGGAATGGCCAAGTATTAGTGCACATGACGGCTACTCTGCTATCTCACTAATTAGGAATAAAATTTGCAAGCTTCCTGAAGAGTTGGTATGTCCGAAACTCCTGATTTTGTTTCTGCAAAGTAATGTTTGGTTGAATGTGATCCCAGAATGTTTCTTTCAGAGTCTGAATGTGTTGAGGGTATTAGATCTAAGCCAAACCAGAATTTCTTTACTACCCACATCATTCAATATCTTGATCAACCTCCAGACCTTGTATCTAGATGGTTGCTTGTCAACCATAGATAACATATCCATACTCGGAAACTTGAAAAAACTTGAGATTCTTAGTATGAAAGAACTTCCTCTTGTGGAACTGCCAAAAGAAATTGGAGATTTGAACCATCTAAGGATGTTAGACATCACTGGTGATTATGTTGATAATGTTCCATCTGAAGTGATATGCAACCTTACTTCGTTAGAGGAACTATACATGCAGTGTAATTTTGCAGACTGGAGGAGTAAAGTTGAGGGAGCAGGAGAAGAAATTATTGTTGCGTTTGATGAGTTAACTGGCTTGTCATATCTAAACGTTTTGAAGGTCTTCATATCTGATGCTAAATGTTTGCCTAGAGATGTGGAGGTTGAGCCAAACTGGGTAAAGTTTGATATTTGTATCAGCAGAGACCCATCAATCAGAAAAGGGTCCTTGGTTTCAAGTTCTTCACCCGCTTTTGTAAGAGCCTTGACTCTTGATACAAGCATCAATGCCTTACCGGGTTGGTTTATCAATGTGGTGACAATGAAAGCAGAGAAACTACAGTACATAATGTGTTGGGGATTAGATAACATTCTTGTGGAATTTGACTGCGGGAGATTACATGCACTGAAGTATTTATCTGTTATTGGGCCCAATGAGAACTTGAAAGTGTTGATGAACACAGTGACACGCTTTCGGAATGAACCAGTGTTTCAAAACTTGGAAGAGTTGCATCTCATTCAAGTGGATTGCCTGAAGGAGTTATGTGTTGGTGAATTGCCACCTGGGTCTCTATGCAATCTTAGGTTACTGAAGGTGAAACATTGCTATGAATTGGTGGATGCACTCCTCCCGTCACAGTTGCTGCAGAGATTACAAAATCTGAAAGAACTGATTTGTGAAGAGATGGATGAATTAAAATATATTTTTGGATGTGAAGAGCTTGAGCCAAAACAGTTGATTTTGACCGAATTGATAGAAATGAGATTGGAGAATCTAGGGAAACTAATAAAGATATGGAATGGTCCTGCTCATTGTGCAGTCTTCCGGAATCTTCAGAGTTTGGTAGTTTCCAGGTGCTTTGAACTGGAGACTCTCTTCACAGCTGATGTAGCTCAATGTTTTTTGCACTTGGGAGATCTTTCAGTCGAGCTTTGCCCAATGTTGGACAGCATTATTGAAGCAAGTAAGGACACAGTAGACAATATGATTGTTTTTCCAGAATTGAAGAACTTAACTTTGATAAAGCTTCCACAACTCACAAGGTTCACAAGGTTCTGTGGTAGTACTGGAAATGCTATTGAGTGCCCTTTACTGGAATACTTGTATGTGGAGAGGTGCCCCCAGTTTTCAACGTCTGACTTCCATAGCAGGAACAAAGTCCAATTCAATCACCCACAACAATTGGACATTGTACAGAGAAGGAGGGACCATGGGGAAATTCGTAAATTGACCGTGCCGGCACCTCAGATCGGGAATACTGAAATCCCACCGGATGATGGCTTCACTTGGCGAAAATACGGCCAGAAGGAGATCATGGGCTGCAGGTTCCCAAGGGCCTACTATAGGTGTAACCACCGAAATTCATACAATTGCCCAGCCACGAAGCAAGTTCAGAGGCTTGACAATGATCACTTAACATTTGAAGTAATAAACAGAGGTGACCATACATGTCATATGTCGTCCACTGCACCCTCAAGCCTACCACCATCTGATCAAACTGTGGAGAGGTTCCCCCAGTTTTCAATGTCTGGCTTCCATAGCAGGAACAAAGTCCAATTCAATCATCCACAACAATTGGACATTGTAAAGAGAAGGAGGGACCGTGGGGAAATTCGTAAATTGACCGTGCCGGCACCTCAGATCGGGAGTACTGAAATCCCACCGGATGATGGCTTCACTTGGCGAAAATACGGCCAGAAGGAGATCATGGGCTGCAGTTTCCCAAGGGGCTACTATAGGTGTACCCACCAAAACTTATACAATTGCCCAGCCAAGAAGGAAGTTCAGAGGCTTGACAATGATCCCTTAACATTTGAAGTAATATACAGAGGTGACCATACATGTCATATGTCTTCCACTGCACCCTCAAGCCTACCACCATCTGATCAAATTGGGAGAGGATCCTCTCCTTAG

Protein Analysis

1574

Amino Acids

178.6

Weight (kDa)

5.66

Isoelectric Point (pI)

47.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 26 - 195 2.1e-35 TIR domain
TIR_2 PF13676 29 - 122 1.2e-11 TIR domain
TIR PF01582 259 - 426 2e-37 TIR domain
TIR_2 PF13676 262 - 357 3.8e-14 TIR domain
NB-ARC PF00931 446 - 610 7.4e-30 NB-ARC domain
LRR_14 PF23598 869 - 944 2e-10 Leucine-rich repeat region
LRR_RPS2 PF23247 1104 - 1249 6.1e-25 Plant disease resistance protein RPS2-like, leucine-rich repeats
WRKY PF03106 1362 - 1422 1.4e-21 WRKY DNA -binding domain
WRKY PF03106 1493 - 1553 1.9e-24 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1059, 1628
Acc36I ACCTGC 2 cut(s) 1996, 4125
Acc65I GGTACC 1 cut(s) 1083
AccB1I GGYRCC 5 cut(s) 335, 1083, 3927, 4051, 4444
AccI GTMKAC 4 cut(s) 363, 699, 1395, 3789
AccIII TCCGGA 1 cut(s) 3636
AciI CCGC 4 cut(s) 200, 714, 3060, 3201
AclI AACGTT 1 cut(s) 2922
AclWI GGATC 9 cut(s) 4, 247, 1575, 1615, 1628, 2488, 4607, 4707, 4720
AcoI YGGCCR 4 cut(s) 2289, 2360, 4111, 4504
AcuI CTGAAG 7 cut(s) 648, 2451, 2805, 3239, 3365, 3422, 3629
AdeI CACNNNGTG 1 cut(s) 1931
AfeI AGCGCT 1 cut(s) 1160
AfiI CCNNNNNNNGG 8 cut(s) 660, 3754, 3904, 4061, 4088, 4155, 4454, 4481
AflII CTTAAG 1 cut(s) 472
AflIII ACRYGT 5 cut(s) 84, 438, 1435, 4259, 4652
AjiI CACGTC 2 cut(s) 85, 441
AjnI CCWGG 5 cut(s) 493, 1032, 2063, 3373, 3662
AjuI GAANNNNNNNTTGG 2 cut(s) 725, 757
Alw21I GWGCWC 1 cut(s) 2377
Alw26I GTCTC 6 cut(s) 216, 1196, 1559, 3009, 3384, 3674
Alw44I GTGCAC 1 cut(s) 2373
AlwI GGATC 9 cut(s) 4, 247, 1575, 1615, 1628, 2488, 4607, 4707, 4720
AlwNI CAGNNNCTG 2 cut(s) 2518, 3454
Aor13HI TCCGGA 1 cut(s) 3636
Aor51HI AGCGCT 1 cut(s) 1160
AoxI GGCC 8 cut(s) 3, 2289, 2317, 2360, 3239, 4111, 4146, 4504
ApaI GGGCCC 1 cut(s) 3243
ApaLI GTGCAC 1 cut(s) 2373
ApeKI GCWGC 6 cut(s) 148, 197, 1961, 3454, 4131, 4524
Asp700I GAANNNNTTC 2 cut(s) 1449, 2933
Asp718I GGTACC 1 cut(s) 1083
AspLEI GCGC 3 cut(s) 917, 1161, 1363
AsuC2I CCSGG 1 cut(s) 3108
AvaII GGWCC 7 cut(s) 451, 623, 1806, 3036, 3614, 4018, 4411
BaeGI GKGCMC 4 cut(s) 2377, 3243, 3899, 3932
BalI TGGCCA 2 cut(s) 2291, 2362
BamHI GGATCC 2 cut(s) 1620, 4712
BanI GGYRCC 5 cut(s) 335, 1083, 3927, 4051, 4444
BanII GRGCYC 1 cut(s) 3243
BarI GAAGNNNNNNTAC 2 cut(s) 808, 840
BauI CACGAG 1 cut(s) 1688
BbsI GAAGAC 8 cut(s) 741, 1035, 1886, 2106, 2127, 2926, 3625, 4656
Bbv12I GWGCWC 1 cut(s) 2377
BbvI GCAGC 6 cut(s) 135, 209, 1973, 3441, 4118, 4511
BceAI ACGGC 7 cut(s) 349, 674, 1370, 1500, 2398, 4126, 4519
BciT130I CCWGG 5 cut(s) 495, 1034, 2065, 3375, 3664
BciVI GTATCC 1 cut(s) 1504
BclI TGATCA 5 cut(s) 1479, 2589, 4219, 4303, 4696
BcnI CCSGG 1 cut(s) 3108
BcoDI GTCTC 6 cut(s) 216, 1196, 1559, 3009, 3384, 3674
BfaI CTAG 9 cut(s) 224, 884, 1131, 1878, 1973, 2190, 2612, 2961, 3587
BfmI CTRYAG 6 cut(s) 3149, 3455, 4132, 4153, 4525, 4546
BfoI RGCGCY 3 cut(s) 918, 1162, 1364
BfrI CTTAAG 1 cut(s) 472
BfuAI ACCTGC 2 cut(s) 1996, 4125
BfuI GTATCC 1 cut(s) 1504
BglII AGATCT 4 cut(s) 187, 886, 2539, 3727
BisI GCNGC 6 cut(s) 149, 198, 1962, 3455, 4132, 4525
BlsI GCNGC 6 cut(s) 150, 199, 1963, 3456, 4133, 4526
BmcAI AGTACT 2 cut(s) 3877, 4462
Bme1390I CCNGG 6 cut(s) 495, 1034, 2065, 3108, 3375, 3664
Bme18I GGWCC 7 cut(s) 451, 623, 1806, 3036, 3614, 4018, 4411
BmgBI CACGTC 2 cut(s) 85, 441
BmrFI CCNGG 6 cut(s) 495, 1034, 2065, 3108, 3375, 3664
BmrI ACTGGG 3 cut(s) 2995, 3928, 4321
BmuI ACTGGG 3 cut(s) 2995, 3928, 4321
BpiI GAAGAC 8 cut(s) 741, 1035, 1886, 2106, 2127, 2926, 3625, 4656
BpmI CTGGAG 3 cut(s) 2582, 2866, 3698
BpuEI CTTGAG 5 cut(s) 1531, 2690, 3554, 4270, 4663
BpuMI CCSGG 1 cut(s) 3108
BsaI GGTCTC 2 cut(s) 3009, 3384
BsaWI WCCGGW 5 cut(s) 1086, 1304, 3636, 4081, 4474
BsaXI ACNNNNNCTCC 2 cut(s) 4399, 4429
Bsc4I CCNNNNNNNGG 8 cut(s) 660, 3754, 3904, 4061, 4088, 4155, 4454, 4481
Bse118I RCCGGY 2 cut(s) 4048, 4441
Bse3DI GCAATG 1 cut(s) 3412
BseAI TCCGGA 1 cut(s) 3636
BseBI CCWGG 5 cut(s) 495, 1034, 2065, 3375, 3664
BseLI CCNNNNNNNGG 8 cut(s) 660, 3754, 3904, 4061, 4088, 4155, 4454, 4481
BseMI GCAATG 1 cut(s) 3412
BseMII CTCAG 3 cut(s) 198, 4070, 4463
BseRI GAGGAG 5 cut(s) 1108, 1574, 1577, 2863, 3428
BseSI GKGCMC 4 cut(s) 2377, 3243, 3899, 3932
BseXI GCAGC 6 cut(s) 135, 209, 1973, 3441, 4118, 4511
BseYI CCCAGC 3 cut(s) 1679, 4186, 4579
BsgI GTGCAG 4 cut(s) 1282, 3648, 4263, 4656
BshFI GGCC 8 cut(s) 5, 2291, 2319, 2362, 3241, 4113, 4148, 4506
BshNI GGYRCC 5 cut(s) 335, 1083, 3927, 4051, 4444
BsiHKAI GWGCWC 1 cut(s) 2377
BsiSI CCGG 8 cut(s) 1087, 1305, 3107, 3637, 4049, 4082, 4442, 4475
BslFI GGGAC 4 cut(s) 115, 2264, 4031, 4424
BslI CCNNNNNNNGG 8 cut(s) 660, 3754, 3904, 4061, 4088, 4155, 4454, 4481
BsmAI GTCTC 6 cut(s) 216, 1196, 1559, 3009, 3384, 3674
BsmFI GGGAC 4 cut(s) 115, 2264, 4031, 4424
BsmI GAATGC 1 cut(s) 869
BsnI GGCC 8 cut(s) 5, 2291, 2319, 2362, 3241, 4113, 4148, 4506
Bso31I GGTCTC 2 cut(s) 3009, 3384
Bsp120I GGGCCC 1 cut(s) 3239
Bsp1286I GDGCHC 4 cut(s) 2377, 3243, 3899, 3932
Bsp13I TCCGGA 1 cut(s) 3636
Bsp1407I TGTACA 2 cut(s) 2332, 4003
Bsp19I CCATGG 3 cut(s) 654, 1425, 4021
BspACI CCGC 4 cut(s) 200, 714, 3060, 3201
BspANI GGCC 8 cut(s) 5, 2291, 2319, 2362, 3241, 4113, 4148, 4506
BspCNI CTCAG 3 cut(s) 197, 4069, 4462
BspEI TCCGGA 1 cut(s) 3636
BspMAI CTGCAG 3 cut(s) 3459, 4136, 4529
BspMI ACCTGC 2 cut(s) 1996, 4125
BspPI GGATC 9 cut(s) 4, 247, 1575, 1615, 1628, 2488, 4607, 4707, 4720
BspQI GCTCTTC 1 cut(s) 3522
BspT107I GGYRCC 5 cut(s) 335, 1083, 3927, 4051, 4444
BspTI CTTAAG 1 cut(s) 472
BspTNI GGTCTC 2 cut(s) 3009, 3384
BsrDI GCAATG 1 cut(s) 3412
BsrFI RCCGGY 2 cut(s) 4048, 4441
BsrGI TGTACA 2 cut(s) 2332, 4003
BssAI RCCGGY 2 cut(s) 4048, 4441
BssSI CACGAG 1 cut(s) 1688
BssT1I CCWWGG 6 cut(s) 654, 1425, 3039, 4021, 4142, 4535
Bst2BI CACGAG 1 cut(s) 1688
Bst2UI CCWGG 5 cut(s) 495, 1034, 2065, 3375, 3664
Bst6I CTCTTC 7 cut(s) 39, 1085, 2427, 3309, 3486, 3522, 3692
BstAFI CTTAAG 1 cut(s) 472
BstAUI TGTACA 2 cut(s) 2332, 4003
BstC8I GCNNGC 5 cut(s) 249, 1206, 2423, 4050, 4443
BstDEI CTNAG 9 cut(s) 116, 184, 2543, 2678, 2738, 3392, 4056, 4449, 4722
BstDSI CCRYGG 4 cut(s) 654, 1425, 4021, 4414
BstEII GGTNACC 2 cut(s) 4250, 4643
BstENI CCTNNNNNAGG 1 cut(s) 4153
BstH2I RGCGCY 3 cut(s) 918, 1162, 1364
BstHHI GCGC 3 cut(s) 917, 1161, 1363
BstMAI GTCTC 6 cut(s) 216, 1196, 1559, 3009, 3384, 3674
BstMWI GCNNNNNNNGC 6 cut(s) 284, 1210, 1910, 3626, 3701, 4350
BstNI CCWGG 5 cut(s) 495, 1034, 2065, 3375, 3664
BstNSI RCATGY 8 cut(s) 1208, 1439, 1478, 1887, 2827, 3215, 4263, 4656
BstPI GGTNACC 2 cut(s) 4250, 4643
BstSCI CCNGG 6 cut(s) 493, 1032, 2063, 3106, 3373, 3662
BstSFI CTRYAG 6 cut(s) 3149, 3455, 4132, 4153, 4525, 4546
BstSLI GKGCMC 4 cut(s) 2377, 3243, 3899, 3932
BstV1I GCAGC 6 cut(s) 135, 209, 1973, 3441, 4118, 4511
BstV2I GAAGAC 8 cut(s) 741, 1035, 1886, 2106, 2127, 2926, 3625, 4656
BstX2I RGATCY 7 cut(s) 187, 886, 1580, 1620, 2539, 3727, 4712
BstYI RGATCY 7 cut(s) 187, 886, 1580, 1620, 2539, 3727, 4712
BsuI GTATCC 1 cut(s) 1504
BsuRI GGCC 8 cut(s) 5, 2291, 2319, 2362, 3241, 4113, 4148, 4506
BtgI CCRYGG 4 cut(s) 654, 1425, 4021, 4414
BtrI CACGTC 2 cut(s) 85, 441
BtsI GCAGTG 5 cut(s) 464, 1380, 2834, 4275, 4668
BveI ACCTGC 2 cut(s) 1996, 4125
Cac8I GCNNGC 5 cut(s) 249, 1206, 2423, 4050, 4443
CaiI CAGNNNCTG 2 cut(s) 2518, 3454
CfoI GCGC 3 cut(s) 917, 1161, 1363
Cfr10I RCCGGY 2 cut(s) 4048, 4441
CspCI CAANNNNNGTGG 4 cut(s) 3975, 4010, 4368, 4403
DdeI CTNAG 9 cut(s) 116, 184, 2543, 2678, 2738, 3392, 4056, 4449, 4722
DraI TTTAAA 1 cut(s) 1176
DraIII CACNNNGTG 1 cut(s) 1931
EaeI YGGCCR 4 cut(s) 2289, 2360, 4111, 4504
Eam1104I CTCTTC 7 cut(s) 39, 1085, 2427, 3309, 3486, 3522, 3692
EarI CTCTTC 7 cut(s) 39, 1085, 2427, 3309, 3486, 3522, 3692
EciI GGCGGA 1 cut(s) 729
Eco130I CCWWGG 6 cut(s) 654, 1425, 3039, 4021, 4142, 4535
Eco24I GRGCYC 1 cut(s) 3243
Eco31I GGTCTC 2 cut(s) 3009, 3384
Eco32I GATATC 1 cut(s) 2110
Eco47I GGWCC 7 cut(s) 451, 623, 1806, 3036, 3614, 4018, 4411
Eco47III AGCGCT 1 cut(s) 1160
Eco57I CTGAAG 7 cut(s) 648, 2451, 2805, 3239, 3365, 3422, 3629
Eco91I GGTNACC 2 cut(s) 4250, 4643
EcoNI CCTNNNNNAGG 1 cut(s) 4153
EcoO109I RGGNCCY 3 cut(s) 623, 3036, 4146
EcoO65I GGTNACC 2 cut(s) 4250, 4643
EcoRI GAATTC 3 cut(s) 342, 1041, 1674
EcoRII CCWGG 5 cut(s) 493, 1032, 2063, 3373, 3662
EcoRV GATATC 1 cut(s) 2110
EcoT14I CCWWGG 6 cut(s) 654, 1425, 3039, 4021, 4142, 4535
EcoT22I ATGCAT 3 cut(s) 871, 2178, 2271
EcoT38I GRGCYC 1 cut(s) 3243
ErhI CCWWGG 6 cut(s) 654, 1425, 3039, 4021, 4142, 4535
FalI AAGNNNNNCTT 2 cut(s) 1819, 1851
FaqI GGGAC 4 cut(s) 115, 2264, 4031, 4424
FauI CCCGC 2 cut(s) 3067, 3194
FauNDI CATATG 2 cut(s) 4266, 4659
FbaI TGATCA 5 cut(s) 1479, 2589, 4219, 4303, 4696
FblI GTMKAC 4 cut(s) 363, 699, 1395, 3789
Fnu4HI GCNGC 6 cut(s) 149, 198, 1962, 3455, 4132, 4525
FriOI GRGCYC 1 cut(s) 3243
Fsp4HI GCNGC 6 cut(s) 149, 198, 1962, 3455, 4132, 4525
FspBI CTAG 9 cut(s) 224, 884, 1131, 1878, 1973, 2190, 2612, 2961, 3587
GlaI GCGC 3 cut(s) 916, 1160, 1362
GluI GCNGC 6 cut(s) 149, 198, 1962, 3455, 4132, 4525
GsaI CCCAGC 3 cut(s) 1683, 4190, 4583
GsuI CTGGAG 3 cut(s) 2582, 2866, 3698
HaeII RGCGCY 3 cut(s) 918, 1162, 1364
HaeIII GGCC 8 cut(s) 5, 2291, 2319, 2362, 3241, 4113, 4148, 4506
HapII CCGG 8 cut(s) 1087, 1305, 3107, 3637, 4049, 4082, 4442, 4475
HhaI GCGC 3 cut(s) 917, 1161, 1363
Hin6I GCGC 3 cut(s) 915, 1159, 1361
HinP1I GCGC 3 cut(s) 915, 1159, 1361
HincII GTYRAC 3 cut(s) 364, 2628, 2901
HindII GTYRAC 3 cut(s) 364, 2628, 2901
HindIII AAGCTT 4 cut(s) 1107, 1211, 2423, 3836
HpaI GTTAAC 1 cut(s) 2901
HpaII CCGG 8 cut(s) 1087, 1305, 3107, 3637, 4049, 4082, 4442, 4475
Hpy99I CGWCG 3 cut(s) 72, 445, 1971
HpyCH4IV ACGT 5 cut(s) 84, 440, 975, 2922, 3944
HpyF10VI GCNNNNNNNGC 6 cut(s) 284, 1210, 1910, 3626, 3701, 4350
HpyF3I CTNAG 9 cut(s) 116, 184, 2543, 2678, 2738, 3392, 4056, 4449, 4722
HpySE526I ACGT 5 cut(s) 84, 440, 975, 2922, 3944
HspAI GCGC 3 cut(s) 915, 1159, 1361
Kpn2I TCCGGA 1 cut(s) 3636
KpnI GGTACC 1 cut(s) 1087
KroI GCCGGC 2 cut(s) 4048, 4441
KroNI GCCGGC 2 cut(s) 4050, 4443
Ksp22I TGATCA 5 cut(s) 1479, 2589, 4219, 4303, 4696
KspAI GTTAAC 1 cut(s) 2901
LguI GCTCTTC 1 cut(s) 3522
LmnI GCTCC 2 cut(s) 298, 2864
Lsp1109I GCAGC 6 cut(s) 135, 209, 1973, 3441, 4118, 4511
MaeI CTAG 9 cut(s) 224, 884, 1131, 1878, 1973, 2190, 2612, 2961, 3587
MaeII ACGT 5 cut(s) 84, 440, 975, 2922, 3944
MaeIII GTNAC 8 cut(s) 1378, 3127, 3274, 3396, 3444, 4160, 4250, 4643
MfeI CAATTG 8 cut(s) 231, 252, 930, 2020, 3992, 4180, 4385, 4573
MflI RGATCY 7 cut(s) 187, 886, 1580, 1620, 2539, 3727, 4712
MhlI GDGCHC 4 cut(s) 2377, 3243, 3899, 3932
MlsI TGGCCA 2 cut(s) 2291, 2362
MluNI TGGCCA 2 cut(s) 2291, 2362
MlyI GAGTC 6 cut(s) 176, 514, 755, 2524, 3073, 3676
MmeI TCCRAC 5 cut(s) 423, 723, 1393, 1689, 3735
Mox20I TGGCCA 2 cut(s) 2291, 2362
Mph1103I ATGCAT 3 cut(s) 871, 2178, 2271
MroI TCCGGA 1 cut(s) 3636
MroNI GCCGGC 2 cut(s) 4048, 4441
MroXI GAANNNNTTC 2 cut(s) 1449, 2933
MscI TGGCCA 2 cut(s) 2291, 2362
MslI CAYNNNNRTG 4 cut(s) 1440, 3185, 3332, 3417
Msp20I TGGCCA 2 cut(s) 2291, 2362
MspA1I CMGCKG 5 cut(s) 47, 148, 200, 764, 3695
MspCI CTTAAG 1 cut(s) 472
MspI CCGG 8 cut(s) 1087, 1305, 3107, 3637, 4049, 4082, 4442, 4475
MspR9I CCNGG 6 cut(s) 495, 1034, 2065, 3108, 3375, 3664
MunI CAATTG 8 cut(s) 231, 252, 930, 2020, 3992, 4180, 4385, 4573
Mva1269I GAATGC 1 cut(s) 869
MvaI CCWGG 5 cut(s) 495, 1034, 2065, 3375, 3664
MwoI GCNNNNNNNGC 6 cut(s) 284, 1210, 1910, 3626, 3701, 4350
NaeI GCCGGC 2 cut(s) 4050, 4443
NciI CCSGG 1 cut(s) 3108
NcoI CCATGG 3 cut(s) 654, 1425, 4021
NdeI CATATG 2 cut(s) 4266, 4659
NgoMIV GCCGGC 2 cut(s) 4048, 4441
NmuCI GTSAC 6 cut(s) 1378, 3127, 3274, 3444, 4250, 4643
NsiI ATGCAT 3 cut(s) 871, 2178, 2271
NspI RCATGY 8 cut(s) 1208, 1439, 1478, 1887, 2827, 3215, 4263, 4656
PaeI GCATGC 1 cut(s) 1208
PciI ACATGT 3 cut(s) 1435, 4259, 4652
PciSI GCTCTTC 1 cut(s) 3522
PctI GAATGC 1 cut(s) 869
PdiI GCCGGC 2 cut(s) 4050, 4443
PdmI GAANNNNTTC 2 cut(s) 1449, 2933
PkrI GCNGC 6 cut(s) 150, 199, 1963, 3456, 4133, 4526
PleI GAGTC 6 cut(s) 176, 514, 754, 2523, 3073, 3676
PpsI GAGTC 6 cut(s) 176, 514, 754, 2523, 3073, 3676
PpuMI RGGWCCY 2 cut(s) 623, 3036
PscI ACATGT 3 cut(s) 1435, 4259, 4652
PsiI TTATAA 2 cut(s) 1059, 1628
Psp1406I AACGTT 1 cut(s) 2922
Psp5II RGGWCCY 2 cut(s) 623, 3036
Psp6I CCWGG 5 cut(s) 493, 1032, 2063, 3373, 3662
PspEI GGTNACC 2 cut(s) 4250, 4643
PspFI CCCAGC 3 cut(s) 1679, 4186, 4579
PspGI CCWGG 5 cut(s) 493, 1032, 2063, 3373, 3662
PspOMI GGGCCC 1 cut(s) 3239
PspPPI RGGWCCY 2 cut(s) 623, 3036
PstI CTGCAG 3 cut(s) 3459, 4136, 4529
PstNI CAGNNNCTG 2 cut(s) 2518, 3454
PsuI RGATCY 7 cut(s) 187, 886, 1580, 1620, 2539, 3727, 4712
PvuII CAGCTG 4 cut(s) 47, 148, 764, 3695
RseI CAYNNNNRTG 4 cut(s) 1440, 3185, 3332, 3417
SalI GTCGAC 1 cut(s) 362
SapI GCTCTTC 1 cut(s) 3522
SatI GCNGC 6 cut(s) 149, 198, 1962, 3455, 4132, 4525
ScaI AGTACT 2 cut(s) 3877, 4462
SchI GAGTC 6 cut(s) 176, 514, 755, 2524, 3073, 3676
ScrFI CCNGG 6 cut(s) 495, 1034, 2065, 3108, 3375, 3664
SduI GDGCHC 4 cut(s) 2377, 3243, 3899, 3932
SfcI CTRYAG 6 cut(s) 3149, 3455, 4132, 4153, 4525, 4546
SinI GGWCC 7 cut(s) 451, 623, 1806, 3036, 3614, 4018, 4411
SmiMI CAYNNNNRTG 4 cut(s) 1440, 3185, 3332, 3417
SmlI CTYRAG 6 cut(s) 472, 1510, 2669, 3533, 4285, 4678
SmoI CTYRAG 6 cut(s) 472, 1510, 2669, 3533, 4285, 4678
SphI GCATGC 1 cut(s) 1208
SsiI CCGC 4 cut(s) 200, 714, 3060, 3201
SspI AATATT 1 cut(s) 1246
SspMI CTAG 9 cut(s) 224, 884, 1131, 1878, 1973, 2190, 2612, 2961, 3587
StyD4I CCNGG 6 cut(s) 493, 1032, 2063, 3106, 3373, 3662
StyI CCWWGG 6 cut(s) 654, 1425, 3039, 4021, 4142, 4535
TaiI ACGT 5 cut(s) 87, 443, 978, 2925, 3947
TaqI TCGA 8 cut(s) 8, 363, 537, 588, 1113, 1557, 1966, 3738
TaqII GACCGA 1 cut(s) 3572
TatI WGTACW 8 cut(s) 568, 595, 1270, 2332, 3153, 3875, 4003, 4460
TseFI GTSAC 6 cut(s) 1378, 3127, 3274, 3444, 4250, 4643
TseI GCWGC 6 cut(s) 148, 197, 1961, 3454, 4131, 4524
Tsp45I GTSAC 6 cut(s) 1378, 3127, 3274, 3444, 4250, 4643
TspGWI ACGGA 4 cut(s) 117, 544, 1413, 2268
Vha464I CTTAAG 1 cut(s) 472
VneI GTGCAC 1 cut(s) 2373
VpaK11BI GGWCC 7 cut(s) 451, 623, 1806, 3036, 3614, 4018, 4411
XagI CCTNNNNNAGG 1 cut(s) 4153
XbaI TCTAGA 1 cut(s) 2611
XceI RCATGY 8 cut(s) 1208, 1439, 1478, 1887, 2827, 3215, 4263, 4656
XmiI GTMKAC 4 cut(s) 363, 699, 1395, 3789
XmnI GAANNNNTTC 2 cut(s) 1449, 2933
XspI CTAG 9 cut(s) 224, 884, 1131, 1878, 1973, 2190, 2612, 2961, 3587
ZrmI AGTACT 2 cut(s) 3877, 4462
Zsp2I ATGCAT 3 cut(s) 871, 2178, 2271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.