Rmu_sc0018807.1_g000005

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0018807.1
OG0000085
Physical Location & Seq
Reverse (-)
16869 .. 29721
12853 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0018807.1_g000005.1.cds

Sequence Viewer

Length: 4980 bp
atggcatcaagctctaaaacagcctttgtatcaacatcagcacgtcattggaagtacgatgtgtttttaagtttccggggtcctgacactcgcaggagtatcacatccgaaatatacgatcgactgcaaaacaggagaggaattaaaacattcatggatgatccagatcttagagtaggggatgttatttctcccagtctcctaacagcaattgaagaatcaaggtttgcaattgttgttctctcaccaaactatgctttttctacttggtgtttggaggagctaacaaagatttgtcaatgcatggaagacaacaacattcttccacttttttataatgtcaatccaactgatgtgagatatcagaagacgacctttaaagatgctttcgctaagcatgaaagctctgggagaaacgaaccagagaaggtgcagcaatggagagctgctttaaataaggtggccactttttctgggtggcatacacagaatttcaagactgacagagaacttgttgatgccattgtggactctgtctgcactaaagtactacctactccaattgagtccaccggagatttcacagtatttgaagcaacaagacaagccatggatcaggttatgaaggcattaaaagataacgaggtcactgccattggggtgtacggcatggggggcgttggaaagacaacccttgtggaacatgtcggtcaacaagctcggaagcaaggcctttttgatcatgtgattatggttagagttaatcaaacccctaatttgaaaaatattcaaggcatattggcagatgggctaggcatcaaattgaagaacgagacagaggatggaagagcttctagattgtcttcggagataatcagaagaaacaaaatccttataatcttggacaacgtttggggtgtaatacatctgccaagcataggaattcccacctacgacattcttcagagttgcaactccaaagtcctacttaccacaaggatttggaatgtttgtcatgtcatgaggagccaagaaaatatcaccctcaatagcctatcggaacaagattcatggacattgtttgtgaaatatgcaagcaagtcttttgaatccacctatttcgaggatgtcgcaaggaaggtagctagagaatgtggtggtctaccaatttcattgatagcagttgcaagggcacttggagataaagacttgacggactgggaacaagcagctcaacgggtaaagaagtcgcaagctgccaaccttgacgataagggagatgtatccgaatgcataaaattaagctatgatttcttgaaagatgaggattccaagtcatgcttcttgctttgttgcctattcccagaaggctatgacatccaaataaaagacttgttcaagtatgggatcgggaaaggattgtttcgagatgctgacacaatccaagaagccagagaaaaagctagtttggtggccaagcacctcaaacattgtagcttgctttcggacagtgaatttgatggatgtgttaggatgcatgatgtcatccgggatacagccattcaaattacacaatctgaagataaggacggatttctggtaaaagctggctgtggtttgaagaattggccaggtcgattgcgtgaaggccactctgtaatttcactcatgaagaacgacattcgcaagctacctgaagagctggtatgtccgaaactccaaattttattactacaagagaatgatgatttagatcagatccctgatgcttttttcgaaaatactagtttactgagaatcgtagatcttagttacactagtatatcatccctatccaagtcgtttattcacctaagcaatctccaaggtttgtatttagattcgtgccagaacatatctgacatttccatactgaaaaattttaagaagcttgagattctcagtatgaaagattaccctctaaaggaattgtcaacagaaataggatatttgaccaatttaagggtgttggatgttagtgaccagggttcagtgggtggaggtatcgtcagaattccatctgaagtgatatcaaaattgcataaattagaagaactctacttgctctgtggattttgggactgggggcttaaagttgagggagaagaaaaaaccaatgttcgctttgatgaggtaactggcttatcatatttgaacagtttgacggttcgcatatctgatgcgaattgcatccctaaaaaggttgaggtcaatccgagttggaccaactttgatatatatattggtagggacaggaacaccagaaatatcatcagagacctacacggtcaagtctctctatatgatcataaatattcgagatctttggttcttgatacaaccattagtatgttaccagcttggtttgtcaacgtagtaacaacgaatacagagaagctaatgtatgtaaacttcacagggataaataacattcttgtggaatatcacaatgggaggttacatagtttgaagcatctctctgttattggtcccagtgagagcttggaagcattgatccatatggacatgagaacaagggttcgaaataaacctgtgttcgagaaattggaagagatgcatctggagagtatggattgcctgaaggtgttatgtgttggtgaattaccatctaggtctctctgcagtctgaaggtattgaaggtatgtgattgtcctaacttggggaatgtcctattaccatcaaaattgttacagagactagtaaatctggagaacctattttgtaagtgtatggctggaatggaatatgtgtttggatgtgaaggatttgagctagaacaatcaaaactgagagagatgaagttgttggagctagatgcagtcaaaggcatatgtaatggtcctgctccacctgcaatgttccaagcacttaagagtttgttcatttgccgttgcaaatttataggaggtctcttcacatttgatgtagctcaatgtctttttcaactggaagacattcttataaagagttgccctaatttggaaagattaatcgaagcaggcaaggaatcagagctctgtaagaagaccgttcttccaaaattgaagtacctagtcttggtggatcttcctatgttgtacggtggtagtgctactattgatatggagtgtccttcattgcaacagttgtatgtgagagactgccctcatttttcagcctctgcttatgacttcgggagcaataaacaagtcaaactcgacgatccactgcattacgtatatcagcaaagcacaagacttggcaaaacttattacccatcaccatggactacccccatctctattttcaagggagtgcagaagatccctacctcggtgaacaatgaattcctgcaggattattggcctgaaatgtcagaactaatggagagacctaatctgatgcacgtgattgctgatatttcggtgaccatacctgaagtcatggagaatgtgcccaaccttttggagaaggtaccaagatcaatagtgaagatgatgcctgaactggtgaagaagacgcctgacttattggaggatttttttggattaatggagaggataactgaagtttggacgaagacgattcctgaaaccatcagttttgagtccaagaggattgctgttgttactggagccaacaaagggattggacttgagattagcaggcaattagcttctaatggagttggggtggtactaacagcaagagatgtgaagagaggcacagaagctgttgaaaagcttaaggcctatggtttctctgatgtggcctttcatcagctagatgtaaataacccaaccaccattgctgctctggcagattttctcaaaacccagtttggaaagcttgacattttggttaataatgcaggagtccttggatccacatacctcaccgataacgtcgtagtggagcctcaagatatcttaggttctacatcactgaaagaagttttagtgcaaacatatgagacggcagaggattgcttaaaaacaaactattatggaatcaagcaactcacagaagcacttcttccgcttcttcaaaaatcagaagaagcaaggatagtcaatgtctcttcagcactgggacagctaaggactaaagctaacccttatcaggtttacaccaagtcaccaggaaatcatctcaggcatccaatcaccggcattatcatcagcttgtccttgggatgcatcaattccatcagaaaccatcttcccttcaatctttatctctttcttctttggaaaatatctcttatgtctcccctgaatgccttcaccaccctccttattctcaaaacgaacatcactcctaacttccaccgcataaccatcaacagaacggctctcaaaatcctcaataacattcaagtttatatctttcaacggaagaatcctcgaactggtgttcaacttatcctccgcttcgttctccaacgcttcccactcttccaaattaggcacattatttccctacccgggcaattccttctacggcaacctcctcgcattgacagtatgcaccagcggaggatagtccctgaggtgctcagtgccatgggagggttccatggtctgaacttgaaagggaaagacgatgaggcggtggcaggaaacagggtcacgaacctcgtccatgacctctgggaaggagacgaacttgtcggtgcagtgggcgagggactcaccgccttgaatgatagcattgcggtgacgacgacgacggctatgcccttggaggatttgcagcttttgacgaatactttgccgacggcgattgagaaggagtcggaggagttttggggctttgggtccggctgcttcttcacgatgttgctccaattggagacagttggggcagcgttcgggttcgggtcggggattggggatggggtgtcagtggcatccatggttgagagagaagggatatga

Protein Analysis

1659

Amino Acids

186.35

Weight (kDa)

6.21

Isoelectric Point (pI)

39.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 336, 896, 3023
AarI CACCTGC 1 cut(s) 2920
AasI GACNNNNNNGTC 1 cut(s) 4709
Acc36I ACCTGC 1 cut(s) 2920
Acc65I GGTACC 1 cut(s) 3514
AccB1I GGYRCC 1 cut(s) 3514
AccI GTMKAC 1 cut(s) 1162
AciI CCGC 7 cut(s) 4079, 4372, 4471, 4576, 4652, 4737, 4757
AclI AACGTT 1 cut(s) 909
AcoI YGGCCR 3 cut(s) 462, 1473, 1628
AcsI RAATTY 8 cut(s) 490, 942, 1514, 1722, 1918, 2052, 2957, 3386
AcuI CTGAAG 9 cut(s) 947, 1599, 1716, 2082, 2660, 2708, 3498, 3627, 4107
AcvI CACGTG 1 cut(s) 3448
AcyI GRCGYC 1 cut(s) 3560
AfaI GTAC 7 cut(s) 56, 549, 665, 3110, 3140, 3516, 3738
AflII CTTAAG 2 cut(s) 2930, 3785
AflIII ACRYGT 1 cut(s) 703
AhlI ACTAGT 3 cut(s) 1784, 1817, 2758
AjiI CACGTC 1 cut(s) 44
AjnI CCWGG 3 cut(s) 1630, 2022, 4180
AjuI GAANNNNNNNTTGG 4 cut(s) 1325, 1357, 2796, 2828
Alw21I GWGCWC 2 cut(s) 3078, 4599
AlwNI CAGNNNCTG 2 cut(s) 3221, 3773
Ama87I CYCGRG 1 cut(s) 4526
AoxI GGCC 8 cut(s) 462, 730, 1473, 1628, 1648, 3404, 3789, 3810
ApeKI GCWGC 8 cut(s) 433, 446, 1229, 1256, 3851, 4795, 4866, 4907
ApoI RAATTY 8 cut(s) 490, 942, 1514, 1722, 1918, 2052, 2957, 3386
ArsI GACNNNNNNTTYG 4 cut(s) 1499, 1531, 4795, 4827
AseI ATTAAT 2 cut(s) 3050, 3590
Asp700I GAANNNNTTC 4 cut(s) 850, 3015, 4071, 4322
Asp718I GGTACC 1 cut(s) 3514
AspS9I GGNCC 5 cut(s) 80, 2262, 2528, 2900, 4860
AsuC2I CCSGG 4 cut(s) 77, 1550, 4527, 4528
AsuII TTCGAA 2 cut(s) 1776, 2581
AvaI CYCGRG 1 cut(s) 4526
AvaII GGWCC 5 cut(s) 80, 2262, 2528, 2900, 4860
AxyI CCTNAGG 1 cut(s) 4590
BaeGI GKGCMC 2 cut(s) 1195, 3498
BalI TGGCCA 3 cut(s) 464, 1475, 1630
BamHI GGATCC 1 cut(s) 3923
BanI GGYRCC 1 cut(s) 3514
BanII GRGCYC 1 cut(s) 3078
BarI GAAGNNNNNNTAC 2 cut(s) 2435, 2467
BbrPI CACGTG 1 cut(s) 3448
BbsI GAAGAC 7 cut(s) 315, 374, 855, 3018, 3092, 3563, 3626
Bbv12I GWGCWC 2 cut(s) 3078, 4599
BbvI GCAGC 8 cut(s) 433, 445, 1241, 1243, 3838, 4807, 4853, 4919
BceAI ACGGC 7 cut(s) 682, 2934, 4032, 4407, 4559, 4788, 4836
BcgI CGANNNNNNTGC 8 cut(s) 632, 666, 4535, 4569, 4759, 4793, 4795, 4829
BciT130I CCWGG 3 cut(s) 1632, 2024, 4182
BciVI GTATCC 2 cut(s) 1294, 1546
BclI TGATCA 2 cut(s) 739, 2344
BcnI CCSGG 4 cut(s) 77, 1550, 4527, 4528
BcuI ACTAGT 3 cut(s) 1784, 1817, 2758
BfmI CTRYAG 2 cut(s) 2680, 3392
BfrI CTTAAG 2 cut(s) 2930, 3785
BfuAI ACCTGC 1 cut(s) 2920
BfuI GTATCC 2 cut(s) 1294, 1546
BglII AGATCT 3 cut(s) 166, 1804, 2360
BisI GCNGC 8 cut(s) 434, 447, 1230, 1257, 3852, 4796, 4867, 4908
BlpI GCTNAGC 1 cut(s) 393
BlsI GCNGC 8 cut(s) 435, 448, 1231, 1258, 3853, 4797, 4868, 4909
BmcAI AGTACT 1 cut(s) 549
Bme1390I CCNGG 7 cut(s) 77, 1550, 1632, 2024, 4182, 4527, 4528
Bme18I GGWCC 5 cut(s) 80, 2262, 2528, 2900, 4860
BmeT110I CYCGRG 1 cut(s) 4526
BmgBI CACGTC 1 cut(s) 44
BmgT120I GGNCC 5 cut(s) 80, 2262, 2528, 2900, 4860
BmiI GGNNCC 9 cut(s) 81, 1028, 2530, 3516, 3676, 3925, 3957, 4616, 4861
BmrFI CCNGG 7 cut(s) 77, 1550, 1632, 2024, 4182, 4527, 4528
BmrI ACTGGG 6 cut(s) 189, 1228, 2131, 2526, 3871, 4139
BmuI ACTGGG 6 cut(s) 189, 1228, 2131, 2526, 3871, 4139
BpiI GAAGAC 7 cut(s) 315, 374, 855, 3018, 3092, 3563, 3626
BpmI CTGGAG 3 cut(s) 2642, 2789, 3693
Bpu10I CCTNAGC 2 cut(s) 1853, 4139
Bpu1102I GCTNAGC 1 cut(s) 393
Bpu14I TTCGAA 2 cut(s) 1776, 2581
BpuEI CTTGAG 3 cut(s) 1952, 3716, 3945
BpuMI CCSGG 4 cut(s) 77, 1550, 4527, 4528
BsaAI YACGTR 2 cut(s) 3277, 3448
BsaBI GATNNNNATC 2 cut(s) 165, 1752
BsaHI GRCGYC 1 cut(s) 3560
BsaI GGTCTC 4 cut(s) 2310, 2679, 2975, 3424
BsaWI WCCGGW 1 cut(s) 572
BsaXI ACNNNNNCTCC 6 cut(s) 2031, 2061, 3717, 3747, 4452, 4482
Bse118I RCCGGY 1 cut(s) 4208
Bse21I CCTNAGG 1 cut(s) 4590
Bse3DI GCAATG 5 cut(s) 443, 2922, 3176, 3846, 4752
Bse8I GATNNNNATC 2 cut(s) 165, 1752
BseBI CCWGG 3 cut(s) 1632, 2024, 4182
BseJI GATNNNNATC 2 cut(s) 165, 1752
BseMI GCAATG 5 cut(s) 443, 2922, 3176, 3846, 4752
BseMII CTCAG 6 cut(s) 1784, 1954, 2840, 4207, 4581, 4612
BseRI GAGGAG 4 cut(s) 293, 1039, 4542, 4856
BseSI GKGCMC 2 cut(s) 1195, 3498
BseXI GCAGC 8 cut(s) 433, 445, 1241, 1243, 3838, 4807, 4853, 4919
BsgI GTGCAG 4 cut(s) 452, 523, 3377, 4737
Bsh1285I CGRYCG 1 cut(s) 121
BshFI GGCC 8 cut(s) 464, 732, 1475, 1630, 1650, 3406, 3791, 3812
BshNI GGYRCC 1 cut(s) 3514
BsiEI CGRYCG 1 cut(s) 121
BsiHKAI GWGCWC 2 cut(s) 3078, 4599
BsiHKCI CYCGRG 1 cut(s) 4526
BsiSI CCGG 6 cut(s) 76, 573, 1549, 4209, 4527, 4863
BslFI GGGAC 6 cut(s) 2132, 2303, 2514, 4146, 4571, 4743
BsmBI CGTCTC 2 cut(s) 4007, 4695
BsmFI GGGAC 6 cut(s) 2132, 2303, 2514, 4146, 4571, 4743
BsmI GAATGC 2 cut(s) 1295, 4324
BsnI GGCC 8 cut(s) 464, 732, 1475, 1630, 1650, 3406, 3791, 3812
Bso31I GGTCTC 4 cut(s) 2310, 2679, 2975, 3424
BsoBI CYCGRG 1 cut(s) 4526
Bsp119I TTCGAA 2 cut(s) 1776, 2581
Bsp1286I GDGCHC 4 cut(s) 1195, 3078, 3498, 4599
Bsp1720I GCTNAGC 1 cut(s) 393
Bsp19I CCATGG 5 cut(s) 609, 3323, 4605, 4618, 4956
BspACI CCGC 7 cut(s) 4079, 4372, 4471, 4576, 4652, 4737, 4757
BspANI GGCC 8 cut(s) 464, 732, 1475, 1630, 1650, 3406, 3791, 3812
BspCNI CTCAG 6 cut(s) 1785, 1953, 2841, 4206, 4582, 4611
BspHI TCATGA 2 cut(s) 1020, 1668
BspLI GGNNCC 9 cut(s) 81, 1028, 2530, 3516, 3676, 3925, 3957, 4616, 4861
BspMAI CTGCAG 2 cut(s) 2684, 3396
BspMI ACCTGC 1 cut(s) 2920
BspQI GCTCTTC 2 cut(s) 841, 1692
BspT104I TTCGAA 2 cut(s) 1776, 2581
BspT107I GGYRCC 1 cut(s) 3514
BspTI CTTAAG 2 cut(s) 2930, 3785
BspTNI GGTCTC 4 cut(s) 2310, 2679, 2975, 3424
BsrDI GCAATG 5 cut(s) 443, 2922, 3176, 3846, 4752
BsrFI RCCGGY 1 cut(s) 4208
BssAI RCCGGY 1 cut(s) 4208
BssNI GRCGYC 1 cut(s) 3560
BssT1I CCWWGG 9 cut(s) 609, 1864, 3323, 3919, 4230, 4605, 4618, 4782, 4956
Bst2UI CCWGG 3 cut(s) 1632, 2024, 4182
Bst6I CTCTTC 7 cut(s) 841, 1692, 2604, 2978, 3752, 4126, 4502
BstACI GRCGYC 1 cut(s) 3560
BstAFI CTTAAG 2 cut(s) 2930, 3785
BstBAI YACGTR 2 cut(s) 3277, 3448
BstBI TTCGAA 2 cut(s) 1776, 2581
BstC8I GCNNGC 7 cut(s) 1096, 1254, 1499, 1609, 1688, 3061, 3707
BstDSI CCRYGG 5 cut(s) 609, 3323, 4605, 4618, 4956
BstEII GGTNACC 1 cut(s) 3466
BstMCI CGRYCG 1 cut(s) 121
BstMWI GCNNNNNNNGC 3 cut(s) 675, 2912, 3857
BstNI CCWGG 3 cut(s) 1632, 2024, 4182
BstNSI RCATGY 1 cut(s) 707
BstPI GGTNACC 1 cut(s) 3466
BstSCI CCNGG 7 cut(s) 75, 1548, 1630, 2022, 4180, 4525, 4526
BstSFI CTRYAG 2 cut(s) 2680, 3392
BstSLI GKGCMC 2 cut(s) 1195, 3498
BstSNI TACGTA 1 cut(s) 3277
BstV1I GCAGC 8 cut(s) 433, 445, 1241, 1243, 3838, 4807, 4853, 4919
BstV2I GAAGAC 7 cut(s) 315, 374, 855, 3018, 3092, 3563, 3626
BstX2I RGATCY 7 cut(s) 166, 1758, 1804, 2360, 3124, 3363, 3923
BstXI CCANNNNNNTGG 2 cut(s) 3324, 3505
BstYI RGATCY 7 cut(s) 166, 1758, 1804, 2360, 3124, 3363, 3923
Bsu36I CCTNAGG 1 cut(s) 4590
BsuI GTATCC 2 cut(s) 1294, 1546
BsuRI GGCC 8 cut(s) 464, 732, 1475, 1630, 1650, 3406, 3791, 3812
BtgI CCRYGG 5 cut(s) 609, 3323, 4605, 4618, 4956
BtrI CACGTC 1 cut(s) 44
BtsI GCAGTG 3 cut(s) 648, 3266, 4725
BveI ACCTGC 1 cut(s) 2920
Cac8I GCNNGC 7 cut(s) 1096, 1254, 1499, 1609, 1688, 3061, 3707
CaiI CAGNNNCTG 2 cut(s) 3221, 3773
CciI TCATGA 2 cut(s) 1020, 1668
Cfr10I RCCGGY 1 cut(s) 4208
Cfr13I GGNCC 5 cut(s) 80, 2262, 2528, 2900, 4860
Cfr9I CCCGGG 1 cut(s) 4526
CseI GACGC 1 cut(s) 3568
Csp6I GTAC 7 cut(s) 55, 548, 664, 3109, 3139, 3515, 3737
CspCI CAANNNNNGTGG 2 cut(s) 678, 713
CviQI GTAC 7 cut(s) 55, 548, 664, 3109, 3139, 3515, 3737
DraI TTTAAA 2 cut(s) 379, 453
DrdI GACNNNNNNGTC 1 cut(s) 4709
DseDI GACNNNNNNGTC 1 cut(s) 4709
EaeI YGGCCR 3 cut(s) 462, 1473, 1628
Eam1104I CTCTTC 7 cut(s) 841, 1692, 2604, 2978, 3752, 4126, 4502
EarI CTCTTC 7 cut(s) 841, 1692, 2604, 2978, 3752, 4126, 4502
Ecl136II GAGCTC 1 cut(s) 3076
Eco105I TACGTA 1 cut(s) 3277
Eco130I CCWWGG 9 cut(s) 609, 1864, 3323, 3919, 4230, 4605, 4618, 4782, 4956
Eco147I AGGCCT 2 cut(s) 732, 3791
Eco24I GRGCYC 1 cut(s) 3078
Eco31I GGTCTC 4 cut(s) 2310, 2679, 2975, 3424
Eco32I GATATC 3 cut(s) 362, 2070, 3967
Eco47I GGWCC 5 cut(s) 80, 2262, 2528, 2900, 4860
Eco53kI GAGCTC 1 cut(s) 3076
Eco57I CTGAAG 9 cut(s) 947, 1599, 1716, 2082, 2660, 2708, 3498, 3627, 4107
Eco72I CACGTG 1 cut(s) 3448
Eco81I CCTNAGG 1 cut(s) 4590
Eco88I CYCGRG 1 cut(s) 4526
Eco91I GGTNACC 1 cut(s) 3466
EcoICRI GAGCTC 1 cut(s) 3076
EcoO109I RGGNCCY 1 cut(s) 80
EcoO65I GGTNACC 1 cut(s) 3466
EcoRI GAATTC 3 cut(s) 942, 2052, 3386
EcoRII CCWGG 3 cut(s) 1630, 2022, 4180
EcoRV GATATC 3 cut(s) 362, 2070, 3967
EcoT14I CCWWGG 9 cut(s) 609, 1864, 3323, 3919, 4230, 4605, 4618, 4782, 4956
EcoT22I ATGCAT 5 cut(s) 305, 1295, 1539, 2619, 4241
EcoT38I GRGCYC 1 cut(s) 3078
ErhI CCWWGG 9 cut(s) 609, 1864, 3323, 3919, 4230, 4605, 4618, 4782, 4956
Esp3I CGTCTC 2 cut(s) 4007, 4695
FaqI GGGAC 6 cut(s) 2132, 2303, 2514, 4146, 4571, 4743
FauNDI CATATG 3 cut(s) 2559, 2891, 4009
FbaI TGATCA 2 cut(s) 739, 2344
FblI GTMKAC 1 cut(s) 1162
Fnu4HI GCNGC 8 cut(s) 434, 447, 1230, 1257, 3852, 4796, 4867, 4908
FriOI GRGCYC 1 cut(s) 3078
Fsp4HI GCNGC 8 cut(s) 434, 447, 1230, 1257, 3852, 4796, 4867, 4908
GluI GCNGC 8 cut(s) 434, 447, 1230, 1257, 3852, 4796, 4867, 4908
GsuI CTGGAG 3 cut(s) 2642, 2789, 3693
HaeIII GGCC 8 cut(s) 464, 732, 1475, 1630, 1650, 3406, 3791, 3812
HapII CCGG 6 cut(s) 76, 573, 1549, 4209, 4527, 4863
HgaI GACGC 1 cut(s) 3568
Hin1I GRCGYC 1 cut(s) 3560
HincII GTYRAC 3 cut(s) 713, 1974, 2410
HindII GTYRAC 3 cut(s) 713, 1974, 2410
HindIII AAGCTT 3 cut(s) 1928, 3782, 3887
HpaII CCGG 6 cut(s) 76, 573, 1549, 4209, 4527, 4863
Hpy99I CGWCG 6 cut(s) 3263, 3950, 4768, 4771, 4774, 4822
HpyCH4IV ACGT 6 cut(s) 43, 909, 2412, 3276, 3447, 3945
HpyF10VI GCNNNNNNNGC 3 cut(s) 675, 2912, 3857
HpySE526I ACGT 6 cut(s) 43, 909, 2412, 3276, 3447, 3945
Hsp92I GRCGYC 1 cut(s) 3560
KpnI GGTACC 1 cut(s) 3518
Ksp22I TGATCA 2 cut(s) 739, 2344
LguI GCTCTTC 2 cut(s) 841, 1692
LmnI GCTCC 8 cut(s) 280, 1026, 2869, 2911, 3237, 3674, 3955, 4890
Lsp1109I GCAGC 8 cut(s) 433, 445, 1241, 1243, 3838, 4807, 4853, 4919
MaeII ACGT 6 cut(s) 43, 909, 2412, 3276, 3447, 3945
MfeI CAATTG 4 cut(s) 210, 231, 561, 4889
MflI RGATCY 7 cut(s) 166, 1758, 1804, 2360, 3124, 3363, 3923
MhlI GDGCHC 4 cut(s) 1195, 3078, 3498, 4599
MlsI TGGCCA 3 cut(s) 464, 1475, 1630
MluNI TGGCCA 3 cut(s) 464, 1475, 1630
MlyI GAGTC 6 cut(s) 524, 575, 3656, 3924, 4725, 4844
MmeI TCCRAC 7 cut(s) 371, 661, 1989, 2240, 2847, 4507, 4818
Mox20I TGGCCA 3 cut(s) 464, 1475, 1630
Mph1103I ATGCAT 5 cut(s) 305, 1295, 1539, 2619, 4241
MroXI GAANNNNTTC 4 cut(s) 850, 3015, 4071, 4322
MscI TGGCCA 3 cut(s) 464, 1475, 1630
MslI CAYNNNNRTG 5 cut(s) 659, 2387, 2474, 3322, 4757
Msp20I TGGCCA 3 cut(s) 464, 1475, 1630
MspA1I CMGCKG 1 cut(s) 4576
MspCI CTTAAG 2 cut(s) 2930, 3785
MspI CCGG 6 cut(s) 76, 573, 1549, 4209, 4527, 4863
MspR9I CCNGG 7 cut(s) 77, 1550, 1632, 2024, 4182, 4527, 4528
MunI CAATTG 4 cut(s) 210, 231, 561, 4889
Mva1269I GAATGC 2 cut(s) 1295, 4324
MvaI CCWGG 3 cut(s) 1632, 2024, 4182
MwoI GCNNNNNNNGC 3 cut(s) 675, 2912, 3857
NciI CCSGG 4 cut(s) 77, 1550, 4527, 4528
NcoI CCATGG 5 cut(s) 609, 3323, 4605, 4618, 4956
NdeI CATATG 3 cut(s) 2559, 2891, 4009
NlaIV GGNNCC 9 cut(s) 81, 1028, 2530, 3516, 3676, 3925, 3957, 4616, 4861
NmuCI GTSAC 6 cut(s) 646, 2018, 3466, 4176, 4669, 4759
NsiI ATGCAT 5 cut(s) 305, 1295, 1539, 2619, 4241
NspI RCATGY 1 cut(s) 707
NspV TTCGAA 2 cut(s) 1776, 2581
PagI TCATGA 2 cut(s) 1020, 1668
PaqCI CACCTGC 1 cut(s) 2920
PceI AGGCCT 2 cut(s) 732, 3791
PciI ACATGT 1 cut(s) 703
PciSI GCTCTTC 2 cut(s) 841, 1692
PctI GAATGC 2 cut(s) 1295, 4324
PdmI GAANNNNTTC 4 cut(s) 850, 3015, 4071, 4322
PflFI GACNNNGTC 1 cut(s) 533
PfoI TCCNGGA 1 cut(s) 1548
PkrI GCNGC 8 cut(s) 435, 448, 1231, 1258, 3853, 4797, 4868, 4909
Ple19I CGATCG 1 cut(s) 121
PleI GAGTC 6 cut(s) 524, 574, 3655, 3923, 4725, 4843
PmaCI CACGTG 1 cut(s) 3448
PmlI CACGTG 1 cut(s) 3448
PpsI GAGTC 6 cut(s) 524, 574, 3655, 3923, 4725, 4843
Ppu21I YACGTR 2 cut(s) 3277, 3448
PpuMI RGGWCCY 1 cut(s) 80
PscI ACATGT 1 cut(s) 703
PshBI ATTAAT 2 cut(s) 3050, 3590
PsiI TTATAA 3 cut(s) 336, 896, 3023
Psp124BI GAGCTC 1 cut(s) 3078
Psp1406I AACGTT 1 cut(s) 909
Psp5II RGGWCCY 1 cut(s) 80
Psp6I CCWGG 3 cut(s) 1630, 2022, 4180
PspCI CACGTG 1 cut(s) 3448
PspEI GGTNACC 1 cut(s) 3466
PspGI CCWGG 3 cut(s) 1630, 2022, 4180
PspN4I GGNNCC 9 cut(s) 81, 1028, 2530, 3516, 3676, 3925, 3957, 4616, 4861
PspPI GGNCC 5 cut(s) 80, 2262, 2528, 2900, 4860
PspPPI RGGWCCY 1 cut(s) 80
PstI CTGCAG 2 cut(s) 2684, 3396
PstNI CAGNNNCTG 2 cut(s) 3221, 3773
PsuI RGATCY 7 cut(s) 166, 1758, 1804, 2360, 3124, 3363, 3923
PsyI GACNNNGTC 1 cut(s) 533
PvuI CGATCG 1 cut(s) 121
RsaI GTAC 7 cut(s) 56, 549, 665, 3110, 3140, 3516, 3738
RsaNI GTAC 7 cut(s) 55, 548, 664, 3109, 3139, 3515, 3737
RseI CAYNNNNRTG 5 cut(s) 659, 2387, 2474, 3322, 4757
SacI GAGCTC 1 cut(s) 3078
SapI GCTCTTC 2 cut(s) 841, 1692
SatI GCNGC 8 cut(s) 434, 447, 1230, 1257, 3852, 4796, 4867, 4908
Sau96I GGNCC 5 cut(s) 80, 2262, 2528, 2900, 4860
SbfI CCTGCAGG 1 cut(s) 3396
ScaI AGTACT 1 cut(s) 549
SchI GAGTC 6 cut(s) 524, 575, 3656, 3924, 4725, 4844
ScrFI CCNGG 7 cut(s) 77, 1550, 1632, 2024, 4182, 4527, 4528
SdaI CCTGCAGG 1 cut(s) 3396
SduI GDGCHC 4 cut(s) 1195, 3078, 3498, 4599
SfcI CTRYAG 2 cut(s) 2680, 3392
SfuI TTCGAA 2 cut(s) 1776, 2581
SinI GGWCC 5 cut(s) 80, 2262, 2528, 2900, 4860
SmaI CCCGGG 1 cut(s) 4528
SmiMI CAYNNNNRTG 5 cut(s) 659, 2387, 2474, 3322, 4757
SmlI CTYRAG 5 cut(s) 1931, 2930, 3695, 3785, 3960
SmoI CTYRAG 5 cut(s) 1931, 2930, 3695, 3785, 3960
SnaBI TACGTA 1 cut(s) 3277
SpeI ACTAGT 3 cut(s) 1784, 1817, 2758
Sse8387I CCTGCAGG 1 cut(s) 3396
SseBI AGGCCT 2 cut(s) 732, 3791
SsiI CCGC 7 cut(s) 4079, 4372, 4471, 4576, 4652, 4737, 4757
SspI AATATT 2 cut(s) 787, 2354
SstI GAGCTC 1 cut(s) 3078
StuI AGGCCT 2 cut(s) 732, 3791
StyD4I CCNGG 7 cut(s) 75, 1548, 1630, 2022, 4180, 4525, 4526
StyI CCWWGG 9 cut(s) 609, 1864, 3323, 3919, 4230, 4605, 4618, 4782, 4956
TaiI ACGT 6 cut(s) 46, 912, 2415, 3279, 3450, 3948
TaqII GACCGA 1 cut(s) 698
TatI WGTACW 1 cut(s) 547
TseFI GTSAC 6 cut(s) 646, 2018, 3466, 4176, 4669, 4759
TseI GCWGC 8 cut(s) 433, 446, 1229, 1256, 3851, 4795, 4866, 4907
Tsp45I GTSAC 6 cut(s) 646, 2018, 3466, 4176, 4669, 4759
TspGWI ACGGA 3 cut(s) 1229, 1605, 4450
TspMI CCCGGG 1 cut(s) 4526
Tth111I GACNNNGTC 1 cut(s) 533
Vha464I CTTAAG 2 cut(s) 2930, 3785
VpaK11BI GGWCC 5 cut(s) 80, 2262, 2528, 2900, 4860
VspI ATTAAT 2 cut(s) 3050, 3590
XapI RAATTY 8 cut(s) 490, 942, 1514, 1722, 1918, 2052, 2957, 3386
XbaI TCTAGA 1 cut(s) 854
XceI RCATGY 1 cut(s) 707
XcmI CCANNNNNNNNNTGG 2 cut(s) 2539, 3853
XmaI CCCGGG 1 cut(s) 4526
XmiI GTMKAC 1 cut(s) 1162
XmnI GAANNNNTTC 4 cut(s) 850, 3015, 4071, 4322
ZrmI AGTACT 1 cut(s) 549
Zsp2I ATGCAT 5 cut(s) 305, 1295, 1539, 2619, 4241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.