Rmu_sc0008050.1_g000015

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008050.1
OG0000085
Physical Location & Seq
Reverse (-)
45044 .. 51645
6602 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008050.1_g000015.1.cds

Sequence Viewer

Length: 5889 bp
atggcttctctgaccaacgaagcagcagccagttcttcatcttcttctctcccactttcttctactgatcaccaaaatcattacacatacgaggttttcctgagtttccgaggcgaggatacgcgctatgattttacagatcatttgtacagagctttgtgtctgaggggaattaagaccttcagagatgatacgcttagcagaggagaagacatatcacaagagctcctcaaagcaattgacgagtccagagtttcaatagtcgtcttctctcaaaactatgcttcctcaaggtggtgtttggatgaactggtcaagatacttgaatgcagaaaatcaaaaagacaagaggtcagaccggttttctacaaagtggctccctcagatgtacgacaccaaagaggcgccttcggtgacgcatttgcaacactcgatcaattttacaaggatagcatggacaaatggaaggcagctctcaaggaagcagcagatttgtccggatggactttcgaggagggccggtacgagactgaatttatcaacgacattgttggggagttgtctacccgagtagtaaacccttcatgtgaatggcacgttgctgaatatccaattggattagagtcttgtatacaagatgtcgaaagtcttttaaaagccgaggaaaatatttttcgcatggtgggcatatgggggactggtggaataggaaagaccacaattgcgaaagctgtgtttaattcaattcgccataagtttgaaactagttgtttcttggcagatgttagatctaatacaaatggcctagcccaactgcaagagagacttttgcatgatcttttaagggactcaaatttgaaggtgagcagtgttgataagggagtcagttttataaaggaaagaatgcaaaataaaaaagttctcttaattcttgatgacgtgagtcgttctagccaattaaagaacttagttccatcccctgattgttttggctcgggcagtagaattctcataacaacaagagaaaaacgttggctaatttctcatgaagttaatgaagtatatgaggtcaagatgttagatgatcgtcatgctttggagttgtttagcttgcatgcattcaaaagaaatgaacctccaagcgattatctggaacttgcacaacatgttatacgctatgcccaaggccttccattagctttgatagtcttaggctctcatctatttcgtagaagcacagaggagtgggaagctacaataggtagttgtaaaggaggtcctcaggcagatatacataaagttctcaaactaagttatgatgctctaggacgagatttaaaagaactatttcttgatatcgcttgcttctttaaaggtgagcgtgcagcccgtgtgaaaccaatactagaagcttgctatgaccacaaaacaatggtgattggtattgcacaactgcaagaaaaagccttgataagaattgacaaagatgatcagatttggatgcatgacttgatagaagaaatgggtacaaatatagtatatcaagagtcacctgatgaaccaggggaacgcagcagactatggagtcaagaagatgtcgactacgttctaacaaataatacaggtacaaataaagttataggcattcaggtccccccgtgccgagcatctacaatatctttgaatgctaaaagcttttcagagatgaagaatcttagatatatttctatgagcagaatatccttttctggagatattgattatctctccaaccaattgaggtggctctcatgtcggttgcaatgttttccatctgattttcaagcaaacaaacttgtggagctcaacattagttggggcaaaattagacgactatgggagggacgtaagaatttttcaaggctaacatgtatgaatttaagtggctgtaagtccttagaggaactcccagacttgagtggaatccctaacttgaaaaggttgaatttaagtggctgtgaatccctaaaggaactcccagacttgagtggaatccctaacttgaaagtgttgaatttaagtggctgtgtatccctaaaggaactcccagacttgagtggaatccctaacttgaatgggttgaatttaagtggctatgaatccctaaagaaactcccagacttgaatggaatccctaacttgaaagagttgaatttaagtgactctaaatccctaaaggaactcccagacttgagtggaatccctaacttgaatgagttgaatttaagtggctgtgaatccctaaagaaactcccagacttgagtggaatccctaacttgaaagagttgaatttaagtggctgtaaatccctaaaggaactcccagagttgagtggaatccctaacttgaaagtgttgaatttaggaggctgtacatccctaaagaaactcccagacttgagtggaatccctaacttaaaagggttgaatttaagtggccgtgaatccctaaaggaactcccggacttgagtagaagacaagccactaatgaaattgagcctacactgccacagctgaccgaaggttttgaagcagttgaagcaacaacacaagccaccaatggaaactctgaaccggcttctcagtggaagcatgatgtgtttttgagttttaggggtgaagacactcgcaagggttttctatcccatttatatcatgaattgcaaaacacgaaagtaattagaacattcaaggatgatgaacaacttgagaaagggaaggatatttctcatagtctgctgacagcaattgaagaatcaaggtttgcgattattgttctgtcggaaaactatgctttttctgcatggtgtttagatgaacttacaaagattgttcaatgcatggaaggcaaggttacaattctgccaattttttatcatgtggctccttctgatatacgacgtcaaaagggtcaatttgaagtggcctttacaaagcatgaaagcgacgcaaggtatgacagagagaaggtgaagcagtggagagctgatttaacaaaagtgggaaatctctctgggtgggattcaaacaaccataagtcagaaagggaactcattgaagacattgtgaaagttgtgctcaaaaaagtatctcaaacaccaaggggagatttcgaagaatttgaagcaacaagagaagccatggatgaggttaaggagatgctgaatgatgataagatcaccgccattggggtctatggaagagaaggcgttggcaagacagccatggtgaaacatgtcggtgcacaagccaaaagaattgggctttttgatcatgttgttctggctgttatatccgaaaaccctgatttgcaaaaaattcaaggcgaattggtaaatcagctgggattggaattggtggtggatgaagaaatagaaagagccagtagattgaaggacaggatcatgggaggaaagaggatccttattattttggacgacatttgggagataatagatttgtcaagcattggaattcctagctatgacgagcttcaaagatgcaattccaaggtcctactcaccacaaggatcaggaatatttgtcaatctatggggtgccaagcacatattcgtctcaatatcctatcagagcaagattctttgaagttattcatgaagaagtcgagaaagccttttcaaggatctactgattcctatgatgaagagaggaacgtagttagcaaatgctctggtctcccaagtgtattggaagaagctgcaagggaatttggacataaatatgtggaagaatggaacaaagcagtttcaaaggcatttggagatgatgatgatgaatttcaaaaagcagctcaagaactaaatgcatctcaacctgtccccactaaagatgggagagatacatttaaagggatagagttaagctatcataccttgaggtttgatgatgccaagtcatttttcttgctttgctgcatgtttccagaagattatgatatcccaattgaagacttgtttaagtatgggattggaaaaggtttgcttcagtattcagacacattgcaagaagccagagacacagcacattctttggtcgcataccttaaagattctgggttgcttttggatggcaagaagggatgtataaagatgaattttgatgtccgaaaggccatctctattgcattgtctgaaggtcatgctttttttgtaaaagctggttgtgaattgaaggattggccaatggatactcatgaacgctactcagcaatctcactaatgaggaacaaacttaacaagcttcccgaaactttggtatgttctgaactccaaattttactgctgcaaagtaatgctggtatatgtgacattccgcaaacattttttcaaagtccaaatgcactaaggatctttgatcttagctataccagcatttcagtactaccctcttcattcagtctcctcaccaacctccaagccttgcatttggattgttgcaaaaagataactgacatatccgtacttgggaagctgaagaaagttgagattcttagtttgagaaaatttcctcttaaggagttgccggaagagataggaaatttgaccagtctaaggatgctggacgtcaccggtggatgtgttcacaaaattccatctgaagtgatatcgcggttgtatagactggaagaattgtacatgaaatgtgagtttggggactggggaagtaaagtcgaggaagcagtaggacaaacaaatgccgactttgatgagataattggcttgtcccatttaaacattttgaaggtttacatatctgatgcagcttgcttacccaaagatattaggtactttgagccgaattgggtaaaatttgacatatgcatcagcagagatctctcttcggaaatgatgctttcttcgaatgatcaaccttcaagaaccttgactcttgacagaacaattaatggcttatcggattggtttatcaacgtggtgacggagaaagcagaaaaactagagtacagagagtgtaagggattaaaaaacattcttaaggaatatgagcatgggagattacatgggctcaggcatctctctgtaattgggtgccatgagagattggaagagctgataaatgtaacaacatgggatccaagtaaacctgtgttcgagaggttggaagagttgcatcttcttcacttgcaatgcctcaaggagttatgtgttggcgaattaccacttgggtctctatgcaatctcaagttattgaaagtgcaaacttgttgtaacttggtgaatgcacttttaccaccaaaattgttgcagagactatccaatctagaaacaatattttgtgtaaatatggaacacatgaaatatgtgtttggatttgaagggcttgagcctgaaatgaatctcttgagaaaattgagcgagatgagattgtgtgctctcttcggactaataagcatctggaatggtcctgctccacctgcagtcttttgtaatcttaaagttttggcagtttgccggtgtacaaaactgaaaagtctattcccattcgatgtggctcgatctcttttgcaattggaagacctctctgtagagcattgccctagcttgggcagagttattgaagcaagcaacagcttgaacagattcatcccttttgtagcattgaagaaattagttttgaaaaatcttccagagctcacatggttctgcaccaaaaatggtactgttgatatcgagtgcccttggctggaatacttgtatgtaaatgagtgccccaagtgcccccagtttataaactctgcttctgcttctgttaaactcaacgatcaacaacatttgaattctctagtgaaaaggtacgtctctgtgtcataa

Protein Analysis

1962

Amino Acids

221.8

Weight (kDa)

5.92

Isoelectric Point (pI)

41.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 893, 5807
AarI CACCTGC 1 cut(s) 5500
AasI GACNNNNNNGTC 1 cut(s) 1584
AatII GACGTC 2 cut(s) 2944, 4626
Acc36I ACCTGC 1 cut(s) 5500
AccB1I GGYRCC 3 cut(s) 404, 3597, 5106
AccI GTMKAC 3 cut(s) 563, 631, 1599
AccII CGCG 2 cut(s) 124, 4669
AccIII TCCGGA 1 cut(s) 497
AciI CCGC 3 cut(s) 3222, 4373, 4669
AclI AACGTT 1 cut(s) 1030
AclWI GGATC 8 cut(s) 3449, 3454, 3467, 3578, 3691, 4415, 5144, 5157
AcoI YGGCCR 2 cut(s) 2476, 4238
AcuI CTGAAG 5 cut(s) 166, 4028, 4212, 4553, 4677
AcyI GRCGYC 3 cut(s) 405, 2941, 4623
AfiI CCNNNNNNNGG 9 cut(s) 115, 294, 3228, 3680, 4524, 4611, 5620, 5621, 5761
AflII CTTAAG 2 cut(s) 4571, 5051
AflIII ACRYGT 3 cut(s) 1165, 1907, 3274
AgeI ACCGGT 2 cut(s) 358, 4628
AhdI GACNNNNNGTC 1 cut(s) 350
AhlI ACTAGT 1 cut(s) 764
AjiI CACGTC 1 cut(s) 940
AjnI CCWGG 1 cut(s) 1561
AjuI GAANNNNNNNTTGG 8 cut(s) 597, 629, 3594, 3626, 3944, 3976, 5223, 5255
Alw21I GWGCWC 6 cut(s) 228, 1845, 3120, 3286, 5452, 5712
Alw26I GTCTC 9 cut(s) 521, 817, 3620, 3740, 4068, 4463, 5250, 5320, 5881
Alw44I GTGCAC 1 cut(s) 3282
AlwI GGATC 8 cut(s) 3449, 3454, 3467, 3578, 3691, 4415, 5144, 5157
Ama87I CYCGRG 2 cut(s) 567, 994
Aor13HI TCCGGA 1 cut(s) 497
AoxI GGCC 7 cut(s) 517, 802, 1186, 2476, 2964, 4170, 4238
ApaLI GTGCAC 1 cut(s) 3282
AseI ATTAAT 1 cut(s) 4962
AsiGI ACCGGT 2 cut(s) 358, 4628
Asp700I GAANNNNTTC 3 cut(s) 1347, 3650, 5657
AspLEI GCGC 2 cut(s) 126, 407
AspS9I GGNCC 5 cut(s) 517, 1277, 1651, 3553, 5480
AsuC2I CCSGG 1 cut(s) 2501
AsuII TTCGAA 2 cut(s) 3153, 4919
AvaI CYCGRG 2 cut(s) 567, 994
AvaII GGWCC 4 cut(s) 1277, 1651, 3553, 5480
AxyI CCTNAGG 1 cut(s) 1281
BaeGI GKGCMC 4 cut(s) 3286, 5756, 5789, 5798
BalI TGGCCA 1 cut(s) 4240
BamHI GGATCC 2 cut(s) 3459, 5149
BanI GGYRCC 3 cut(s) 404, 3597, 5106
BanII GRGCYC 4 cut(s) 228, 1845, 5085, 5712
BbsI GAAGAC 7 cut(s) 216, 259, 2521, 2667, 3105, 4014, 5598
Bbv12I GWGCWC 6 cut(s) 228, 1845, 3120, 3286, 5452, 5712
BccI CCATC 7 cut(s) 495, 982, 1819, 3884, 4121, 4181, 4660
BceAI ACGGC 1 cut(s) 2463
BciT130I CCWGG 1 cut(s) 1563
BciVI GTATCC 3 cut(s) 112, 2080, 4240
BclI TGATCA 4 cut(s) 67, 1489, 3310, 4924
BcnI CCSGG 1 cut(s) 2501
BcoDI GTCTC 9 cut(s) 521, 817, 3620, 3740, 4068, 4463, 5250, 5320, 5881
BcuI ACTAGT 1 cut(s) 764
BfmI CTRYAG 2 cut(s) 5493, 5601
BfoI RGCGCY 1 cut(s) 408
BfrI CTTAAG 2 cut(s) 4571, 5051
BfuAI ACCTGC 1 cut(s) 5500
BfuI GTATCC 3 cut(s) 112, 2080, 4240
BglII AGATCT 2 cut(s) 788, 4891
BlpI GCTNAGC 1 cut(s) 197
BmcAI AGTACT 1 cut(s) 4440
Bme1390I CCNGG 2 cut(s) 1563, 2501
Bme18I GGWCC 4 cut(s) 1277, 1651, 3553, 5480
BmeRI GACNNNNNGTC 1 cut(s) 350
BmeT110I CYCGRG 2 cut(s) 567, 994
BmgBI CACGTC 1 cut(s) 940
BmgT120I GGNCC 5 cut(s) 517, 1277, 1651, 3553, 5480
BmiI GGNNCC 8 cut(s) 378, 406, 1653, 2925, 3461, 3599, 5108, 5151
BmrFI CCNGG 2 cut(s) 1563, 2501
BmrI ACTGGG 2 cut(s) 4726, 5794
BmuI ACTGGG 2 cut(s) 4726, 5794
BoxI GACNNNNGTC 1 cut(s) 942
BpiI GAAGAC 7 cut(s) 216, 259, 2521, 2667, 3105, 4014, 5598
BpmI CTGGAG 1 cut(s) 1770
Bpu10I CCTNAGC 1 cut(s) 5084
Bpu1102I GCTNAGC 1 cut(s) 197
Bpu14I TTCGAA 2 cut(s) 3153, 4919
BpuMI CCSGG 1 cut(s) 2501
BsaHI GRCGYC 3 cut(s) 405, 2941, 4623
BsaI GGTCTC 2 cut(s) 3740, 5250
BsaJI CCNNGG 9 cut(s) 109, 660, 1183, 1562, 3140, 3180, 3264, 3549, 5756
BsaWI WCCGGW 3 cut(s) 358, 497, 4628
BsaXI ACNNNNNCTCC 4 cut(s) 210, 240, 506, 536
Bsc4I CCNNNNNNNGG 9 cut(s) 115, 294, 3228, 3680, 4524, 4611, 5620, 5621, 5761
Bse118I RCCGGY 5 cut(s) 358, 519, 2614, 4628, 5529
Bse1I ACTGG 8 cut(s) 30, 315, 703, 3423, 4605, 4686, 4721, 5800
Bse21I CCTNAGG 1 cut(s) 1281
Bse3DI GCAATG 4 cut(s) 1808, 4058, 5210, 5608
BseAI TCCGGA 1 cut(s) 497
BseBI CCWGG 1 cut(s) 1563
BseDI CCNNGG 9 cut(s) 109, 660, 1183, 1562, 3140, 3180, 3264, 3549, 5756
BseLI CCNNNNNNNGG 9 cut(s) 115, 294, 3228, 3680, 4524, 4611, 5620, 5621, 5761
BseMI GCAATG 4 cut(s) 1808, 4058, 5210, 5608
BseMII CTCAG 7 cut(s) 92, 155, 396, 1295, 2636, 4278, 5098
BseNI ACTGG 8 cut(s) 30, 315, 703, 3423, 4605, 4686, 4721, 5800
BseRI GAGGAG 5 cut(s) 218, 219, 527, 1256, 4451
BseSI GKGCMC 4 cut(s) 3286, 5756, 5789, 5798
BseYI CCCAGC 1 cut(s) 3382
BsgI GTGCAG 2 cut(s) 1404, 5707
Bsh1236I CGCG 2 cut(s) 124, 4669
BshFI GGCC 7 cut(s) 519, 804, 1188, 2478, 2966, 4172, 4240
BshNI GGYRCC 3 cut(s) 404, 3597, 5106
BshTI ACCGGT 2 cut(s) 358, 4628
BsiHKAI GWGCWC 6 cut(s) 228, 1845, 3120, 3286, 5452, 5712
BsiHKCI CYCGRG 2 cut(s) 567, 994
BsiSI CCGG 8 cut(s) 359, 498, 520, 2501, 2615, 4583, 4629, 5530
BslFI GGGAC 7 cut(s) 709, 860, 1637, 1896, 3863, 4727, 4768
BslI CCNNNNNNNGG 9 cut(s) 115, 294, 3228, 3680, 4524, 4611, 5620, 5621, 5761
BsmAI GTCTC 9 cut(s) 521, 817, 3620, 3740, 4068, 4463, 5250, 5320, 5881
BsmBI CGTCTC 2 cut(s) 3620, 5881
BsmFI GGGAC 7 cut(s) 709, 860, 1637, 1896, 3863, 4727, 4768
BsmI GAATGC 6 cut(s) 332, 909, 1118, 1644, 1690, 5302
BsnI GGCC 7 cut(s) 519, 804, 1188, 2478, 2966, 4172, 4240
Bso31I GGTCTC 2 cut(s) 3740, 5250
BsoBI CYCGRG 2 cut(s) 567, 994
Bsp119I TTCGAA 2 cut(s) 3153, 4919
Bsp13I TCCGGA 1 cut(s) 497
Bsp1407I TGTACA 4 cut(s) 147, 2411, 4692, 5534
Bsp1720I GCTNAGC 1 cut(s) 197
Bsp19I CCATGG 2 cut(s) 3180, 3264
BspACI CCGC 3 cut(s) 3222, 4373, 4669
BspANI GGCC 7 cut(s) 519, 804, 1188, 2478, 2966, 4172, 4240
BspCNI CTCAG 7 cut(s) 93, 156, 395, 1294, 2635, 4277, 5097
BspEI TCCGGA 1 cut(s) 497
BspFNI CGCG 2 cut(s) 124, 4669
BspHI TCATGA 4 cut(s) 1045, 2695, 3654, 4252
BspLI GGNNCC 8 cut(s) 378, 406, 1653, 2925, 3461, 3599, 5108, 5151
BspMAI CTGCAG 1 cut(s) 5497
BspMI ACCTGC 1 cut(s) 5500
BspPI GGATC 8 cut(s) 3449, 3454, 3467, 3578, 3691, 4415, 5144, 5157
BspQI GCTCTTC 1 cut(s) 5118
BspT104I TTCGAA 2 cut(s) 3153, 4919
BspT107I GGYRCC 3 cut(s) 404, 3597, 5106
BspTI CTTAAG 2 cut(s) 4571, 5051
BspTNI GGTCTC 2 cut(s) 3740, 5250
BsrDI GCAATG 4 cut(s) 1808, 4058, 5210, 5608
BsrFI RCCGGY 5 cut(s) 358, 519, 2614, 4628, 5529
BsrGI TGTACA 4 cut(s) 147, 2411, 4692, 5534
BsrI ACTGG 8 cut(s) 30, 315, 703, 3423, 4605, 4686, 4721, 5800
BssAI RCCGGY 5 cut(s) 358, 519, 2614, 4628, 5529
BssECI CCNNGG 9 cut(s) 109, 660, 1183, 1562, 3140, 3180, 3264, 3549, 5756
BssNAI GTATAC 1 cut(s) 632
BssNI GRCGYC 3 cut(s) 405, 2941, 4623
BssT1I CCWWGG 6 cut(s) 1183, 3140, 3180, 3264, 3549, 5756
Bst1107I GTATAC 1 cut(s) 632
Bst2UI CCWGG 1 cut(s) 1563
Bst4CI ACNGT 1 cut(s) 5740
Bst6I CTCTTC 8 cut(s) 3235, 3699, 4453, 4581, 4903, 5118, 5175, 5459
BstACI GRCGYC 3 cut(s) 405, 2941, 4623
BstAFI CTTAAG 2 cut(s) 4571, 5051
BstAUI TGTACA 4 cut(s) 147, 2411, 4692, 5534
BstBI TTCGAA 2 cut(s) 3153, 4919
BstC8I GCNNGC 7 cut(s) 1112, 1116, 1363, 1383, 1414, 4825, 5641
BstDSI CCRYGG 2 cut(s) 3180, 3264
BstENI CCTNNNNNAGG 1 cut(s) 3678
BstFNI CGCG 2 cut(s) 124, 4669
BstH2I RGCGCY 1 cut(s) 408
BstHHI GCGC 2 cut(s) 126, 407
BstMAI GTCTC 9 cut(s) 521, 817, 3620, 3740, 4068, 4463, 5250, 5320, 5881
BstMWI GCNNNNNNNGC 8 cut(s) 684, 2545, 2579, 2840, 2886, 4428, 5492, 5793
BstNI CCWGG 1 cut(s) 1563
BstNSI RCATGY 5 cut(s) 1118, 1169, 1911, 3278, 3979
BstPAI GACNNNNGTC 1 cut(s) 942
BstSCI CCNGG 2 cut(s) 1561, 2499
BstSFI CTRYAG 2 cut(s) 5493, 5601
BstSLI GKGCMC 4 cut(s) 3286, 5756, 5789, 5798
BstUI CGCG 2 cut(s) 124, 4669
BstV2I GAAGAC 7 cut(s) 216, 259, 2521, 2667, 3105, 4014, 5598
BstX2I RGATCY 6 cut(s) 788, 3459, 3683, 4407, 4891, 5149
BstYI RGATCY 6 cut(s) 788, 3459, 3683, 4407, 4891, 5149
BstZ17I GTATAC 1 cut(s) 632
Bsu36I CCTNAGG 1 cut(s) 1281
BsuI GTATCC 3 cut(s) 112, 2080, 4240
BsuRI GGCC 7 cut(s) 519, 804, 1188, 2478, 2966, 4172, 4240
BtgI CCRYGG 2 cut(s) 3180, 3264
BtrI CACGTC 1 cut(s) 940
BtsI GCAGTG 3 cut(s) 874, 2543, 3023
BtsIMutI CAGTG 4 cut(s) 874, 2543, 2630, 3023
BveI ACCTGC 1 cut(s) 5500
Cac8I GCNNGC 7 cut(s) 1112, 1116, 1363, 1383, 1414, 4825, 5641
CciI TCATGA 4 cut(s) 1045, 2695, 3654, 4252
CfoI GCGC 2 cut(s) 126, 407
Cfr10I RCCGGY 5 cut(s) 358, 519, 2614, 4628, 5529
Cfr13I GGNCC 5 cut(s) 517, 1277, 1651, 3553, 5480
CseI GACGC 2 cut(s) 425, 2996
CspAI ACCGGT 2 cut(s) 358, 4628
CspCI CAANNNNNGTGG 4 cut(s) 1763, 1798, 5298, 5333
DinI GGCGCC 1 cut(s) 406
DraI TTTAAA 5 cut(s) 654, 1338, 1372, 3907, 4791
DrdI GACNNNNNNGTC 1 cut(s) 1584
DriI GACNNNNNGTC 1 cut(s) 350
DseDI GACNNNNNNGTC 1 cut(s) 1584
EaeI YGGCCR 2 cut(s) 2476, 4238
Eam1104I CTCTTC 8 cut(s) 3235, 3699, 4453, 4581, 4903, 5118, 5175, 5459
Eam1105I GACNNNNNGTC 1 cut(s) 350
EarI CTCTTC 8 cut(s) 3235, 3699, 4453, 4581, 4903, 5118, 5175, 5459
Ecl136II GAGCTC 3 cut(s) 226, 1843, 5710
Eco130I CCWWGG 6 cut(s) 1183, 3140, 3180, 3264, 3549, 5756
Eco147I AGGCCT 1 cut(s) 1188
Eco24I GRGCYC 4 cut(s) 228, 1845, 5085, 5712
Eco31I GGTCTC 2 cut(s) 3740, 5250
Eco32I GATATC 4 cut(s) 1357, 3997, 4665, 5746
Eco47I GGWCC 4 cut(s) 1277, 1651, 3553, 5480
Eco53kI GAGCTC 3 cut(s) 226, 1843, 5710
Eco57I CTGAAG 5 cut(s) 166, 4028, 4212, 4553, 4677
Eco81I CCTNAGG 1 cut(s) 1281
Eco88I CYCGRG 2 cut(s) 567, 994
EcoICRI GAGCTC 3 cut(s) 226, 1843, 5710
EcoNI CCTNNNNNAGG 1 cut(s) 3678
EcoO109I RGGNCCY 3 cut(s) 1277, 1651, 3553
EcoRI GAATTC 3 cut(s) 1005, 3513, 5854
EcoRII CCWGG 1 cut(s) 1561
EcoRV GATATC 4 cut(s) 1357, 3997, 4665, 5746
EcoT14I CCWWGG 6 cut(s) 1183, 3140, 3180, 3264, 3549, 5756
EcoT22I ATGCAT 5 cut(s) 1120, 1506, 2882, 3868, 4883
EcoT38I GRGCYC 4 cut(s) 228, 1845, 5085, 5712
EgeI GGCGCC 1 cut(s) 406
EheI GGCGCC 1 cut(s) 406
ErhI CCWWGG 6 cut(s) 1183, 3140, 3180, 3264, 3549, 5756
Esp3I CGTCTC 2 cut(s) 3620, 5881
FalI AAGNNNNNCTT 4 cut(s) 810, 842, 4026, 4058
FaqI GGGAC 7 cut(s) 709, 860, 1637, 1896, 3863, 4727, 4768
FauNDI CATATG 2 cut(s) 689, 4877
FbaI TGATCA 4 cut(s) 67, 1489, 3310, 4924
FblI GTMKAC 3 cut(s) 563, 631, 1599
FriOI GRGCYC 4 cut(s) 228, 1845, 5085, 5712
GlaI GCGC 2 cut(s) 125, 406
GsaI CCCAGC 1 cut(s) 3386
GsuI CTGGAG 1 cut(s) 1770
HaeII RGCGCY 1 cut(s) 408
HaeIII GGCC 7 cut(s) 519, 804, 1188, 2478, 2966, 4172, 4240
HapII CCGG 8 cut(s) 359, 498, 520, 2501, 2615, 4583, 4629, 5530
HgaI GACGC 2 cut(s) 425, 2996
HhaI GCGC 2 cut(s) 126, 407
Hin1I GRCGYC 3 cut(s) 405, 2941, 4623
Hin6I GCGC 2 cut(s) 124, 405
HinP1I GCGC 2 cut(s) 124, 405
HincII GTYRAC 1 cut(s) 1600
HindII GTYRAC 1 cut(s) 1600
HindIII AAGCTT 3 cut(s) 1410, 1693, 4298
HpaII CCGG 8 cut(s) 359, 498, 520, 2501, 2615, 4583, 4629, 5530
Hpy166II GTNNAC 9 cut(s) 564, 577, 632, 1600, 3284, 4642, 4807, 5159, 5535
Hpy8I GTNNAC 9 cut(s) 564, 577, 632, 1600, 3284, 4642, 4807, 5159, 5535
Hpy99I CGWCG 2 cut(s) 2943, 2990
HpyCH4III ACNGT 1 cut(s) 5740
HpyF10VI GCNNNNNNNGC 8 cut(s) 684, 2545, 2579, 2840, 2886, 4428, 5492, 5793
Hsp92I GRCGYC 3 cut(s) 405, 2941, 4623
HspAI GCGC 2 cut(s) 124, 405
KasI GGCGCC 1 cut(s) 404
Kpn2I TCCGGA 1 cut(s) 497
Ksp22I TGATCA 4 cut(s) 67, 1489, 3310, 4924
LguI GCTCTTC 1 cut(s) 5118
LmnI GCTCC 5 cut(s) 231, 382, 1840, 2929, 5491
MaeIII GTNAC 9 cut(s) 413, 1548, 2198, 2893, 4364, 4624, 4993, 5137, 5285
MfeI CAATTG 7 cut(s) 237, 612, 720, 1775, 2787, 4002, 5585
MflI RGATCY 6 cut(s) 788, 3459, 3683, 4407, 4891, 5149
MlsI TGGCCA 1 cut(s) 4240
MluNI TGGCCA 1 cut(s) 4240
Mly113I GGCGCC 1 cut(s) 405
MlyI GAGTC 9 cut(s) 254, 632, 842, 891, 952, 1556, 1594, 2195, 4939
MmeI TCCRAC 3 cut(s) 1794, 2802, 5157
Mox20I TGGCCA 1 cut(s) 4240
Mph1103I ATGCAT 5 cut(s) 1120, 1506, 2882, 3868, 4883
MroI TCCGGA 1 cut(s) 497
MroXI GAANNNNTTC 3 cut(s) 1347, 3650, 5657
MscI TGGCCA 1 cut(s) 4240
MslI CAYNNNNRTG 3 cut(s) 589, 3279, 3780
Msp20I TGGCCA 1 cut(s) 4240
MspA1I CMGCKG 2 cut(s) 2554, 3382
MspCI CTTAAG 2 cut(s) 4571, 5051
MspI CCGG 8 cut(s) 359, 498, 520, 2501, 2615, 4583, 4629, 5530
MspR9I CCNGG 2 cut(s) 1563, 2501
MunI CAATTG 7 cut(s) 237, 612, 720, 1775, 2787, 4002, 5585
Mva1269I GAATGC 6 cut(s) 332, 909, 1118, 1644, 1690, 5302
MvaI CCWGG 1 cut(s) 1563
MvnI CGCG 2 cut(s) 124, 4669
MwoI GCNNNNNNNGC 8 cut(s) 684, 2545, 2579, 2840, 2886, 4428, 5492, 5793
NarI GGCGCC 1 cut(s) 405
NciI CCSGG 1 cut(s) 2501
NcoI CCATGG 2 cut(s) 3180, 3264
NdeI CATATG 2 cut(s) 689, 4877
NlaIV GGNNCC 8 cut(s) 378, 406, 1653, 2925, 3461, 3599, 5108, 5151
NmeAIII GCCGAG 2 cut(s) 685, 1688
NmuCI GTSAC 6 cut(s) 413, 1548, 2198, 4364, 4624, 4993
NsiI ATGCAT 5 cut(s) 1120, 1506, 2882, 3868, 4883
NspI RCATGY 5 cut(s) 1118, 1169, 1911, 3278, 3979
NspV TTCGAA 2 cut(s) 3153, 4919
PaeI GCATGC 1 cut(s) 1118
PagI TCATGA 4 cut(s) 1045, 2695, 3654, 4252
PaqCI CACCTGC 1 cut(s) 5500
PceI AGGCCT 1 cut(s) 1188
PciI ACATGT 3 cut(s) 1165, 1907, 3274
PciSI GCTCTTC 1 cut(s) 5118
PctI GAATGC 6 cut(s) 332, 909, 1118, 1644, 1690, 5302
PdmI GAANNNNTTC 3 cut(s) 1347, 3650, 5657
PfoI TCCNGGA 1 cut(s) 2499
PinAI ACCGGT 2 cut(s) 358, 4628
PleI GAGTC 9 cut(s) 253, 631, 842, 890, 951, 1555, 1593, 2195, 4939
PluTI GGCGCC 1 cut(s) 408
PpsI GAGTC 9 cut(s) 253, 631, 842, 890, 951, 1555, 1593, 2195, 4939
PpuMI RGGWCCY 3 cut(s) 1277, 1651, 3553
PscI ACATGT 3 cut(s) 1165, 1907, 3274
PshAI GACNNNNGTC 1 cut(s) 942
PshBI ATTAAT 1 cut(s) 4962
PsiI TTATAA 2 cut(s) 893, 5807
Psp124BI GAGCTC 3 cut(s) 228, 1845, 5712
Psp1406I AACGTT 1 cut(s) 1030
Psp5II RGGWCCY 3 cut(s) 1277, 1651, 3553
Psp6I CCWGG 1 cut(s) 1561
PspFI CCCAGC 1 cut(s) 3382
PspGI CCWGG 1 cut(s) 1561
PspN4I GGNNCC 8 cut(s) 378, 406, 1653, 2925, 3461, 3599, 5108, 5151
PspPI GGNCC 5 cut(s) 517, 1277, 1651, 3553, 5480
PspPPI RGGWCCY 3 cut(s) 1277, 1651, 3553
PstI CTGCAG 1 cut(s) 5497
PsuI RGATCY 6 cut(s) 788, 3459, 3683, 4407, 4891, 5149
PvuII CAGCTG 2 cut(s) 2554, 3382
RseI CAYNNNNRTG 3 cut(s) 589, 3279, 3780
SacI GAGCTC 3 cut(s) 228, 1845, 5712
SalI GTCGAC 1 cut(s) 1598
SapI GCTCTTC 1 cut(s) 5118
Sau96I GGNCC 5 cut(s) 517, 1277, 1651, 3553, 5480
ScaI AGTACT 1 cut(s) 4440
SchI GAGTC 9 cut(s) 254, 632, 842, 891, 952, 1556, 1594, 2195, 4939
ScrFI CCNGG 2 cut(s) 1563, 2501
SfcI CTRYAG 2 cut(s) 5493, 5601
SfoI GGCGCC 1 cut(s) 406
SfuI TTCGAA 2 cut(s) 3153, 4919
SgrAI CRCCGGYG 1 cut(s) 4628
SinI GGWCC 4 cut(s) 1277, 1651, 3553, 5480
SmiMI CAYNNNNRTG 3 cut(s) 589, 3279, 3780
SpeI ACTAGT 1 cut(s) 764
SphI GCATGC 1 cut(s) 1118
SseBI AGGCCT 1 cut(s) 1188
SsiI CCGC 3 cut(s) 3222, 4373, 4669
SspDI GGCGCC 1 cut(s) 404
SspI AATATT 3 cut(s) 670, 3580, 5349
SstI GAGCTC 3 cut(s) 228, 1845, 5712
StuI AGGCCT 1 cut(s) 1188
StyD4I CCNGG 2 cut(s) 1561, 2499
StyI CCWWGG 6 cut(s) 1183, 3140, 3180, 3264, 3549, 5756
TaaI ACNGT 1 cut(s) 5740
TaqII GACCGA 1 cut(s) 2573
TatI WGTACW 6 cut(s) 147, 2411, 4438, 4692, 5019, 5534
TscAI CASTG 4 cut(s) 874, 2550, 2630, 3023
TseFI GTSAC 6 cut(s) 413, 1548, 2198, 4364, 4624, 4993
Tsp45I GTSAC 6 cut(s) 413, 1548, 2198, 4364, 4624, 4993
TspGWI ACGGA 2 cut(s) 4507, 5012
TspRI CASTG 4 cut(s) 874, 2550, 2630, 3023
Vha464I CTTAAG 2 cut(s) 4571, 5051
VneI GTGCAC 1 cut(s) 3282
VpaK11BI GGWCC 4 cut(s) 1277, 1651, 3553, 5480
VspI ATTAAT 1 cut(s) 4962
XagI CCTNNNNNAGG 1 cut(s) 3678
XbaI TCTAGA 1 cut(s) 5338
XceI RCATGY 5 cut(s) 1118, 1169, 1911, 3278, 3979
XcmI CCANNNNNNNNNTGG 1 cut(s) 5712
XmiI GTMKAC 3 cut(s) 563, 631, 1599
XmnI GAANNNNTTC 3 cut(s) 1347, 3650, 5657
ZraI GACGTC 2 cut(s) 2942, 4624
ZrmI AGTACT 1 cut(s) 4440
Zsp2I ATGCAT 5 cut(s) 1120, 1506, 2882, 3868, 4883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.