Rw3G013910

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000085
Physical Location & Seq
Reverse (-)
13652050 .. 13656382
4333 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G013910.1

Sequence Viewer

Length: 3288 bp
ATGGCCTCAAGCTCCCAACTAGCATATGCAGCATCAATTCCTTCGTCAGCTCCTCGTTGGAAGTATGATGTGTTTTTAAGTTTTAGGGGTAAAGATACTCGCAAGGGTATTACATTCGAATTATACAATCGACTGCAAAATAGGAGTGGAATTAAAACATTCATGGATGACCAAGACCTTGAAGTCGGGGATGTTATTTCTCCTACTCTCCTAACGGCAATTGAAGAATCAAGGTTTGCAATTGTAGTTCTCTCACTAAACTATGCTTCTTCTCCATGGTGTTTGGTGGAACTTACAAAGATATGTCAATGCATGAAAGACAATAATAGAATTCTGCCACTTTTTTATCACGTCGATCCCTCTGATGTACGATATCAGAAGAGGAGTTTTGAGGAGGCTTTCACTAAGCATGAAATCTCTGGGCGACACAGAGAAGAGGTGCAGCAATGGAGAGATGCTTTGAAGAAAGTGGCCAATTTCTCTGGTTGGTATACACAGAATTATAAAACTGAAAGAGAACTTGTTGCAGCCATTGTGGAATCTATGTGCAGTAAAATACAACCTAGTGAAATTGAGTTTACAATGCCCAAGGGAGATTTTGAAGCATATGAAGCAACAACACAAGCCATGGATAAGGTTATGAAGGCGCTACAAGATGATGAGGTCACTGTCGTAGGAGTCTATGGAATGGGAGGTATCGGAAAGACGACCATGGTGGAACATGTCGGTGCACAAGCCTGCAATACTGGGCTTTTTGATCATGTTATTATGGCCGTTGTATCCCAAAGCCCTGACTTCAGAAAAATTCAAGGCACATTGGCAGATCTGTTGGAATTGAAATTAGAGGGGGAGACAGAAGTTGGAAGAGCTTGTAGATTACAGAAGGAGATAATTAACTTGTCAAGAATGGGAATTCCTAGCTATGACCAGCTCCAAAGACGCAATTCCAAAGTCCTTCTCACCACAAGGAGATTTAATGTTTGTCATTCCATGGAGAGCCAGTCAAGCATCCCTCTCAATATCTTATCAGAAGAAGATTCTTGGAAATTGTTTGTGAAGAAAGCAAGAAAGTCTTTTGAAAATTCGACTAATTTCTATGATATTGCAAGGAAGGTAGCAAGAGAATGTGCTGGTCTACCAGTTGCGTTAATAGCAGTTGCGAGGGCACTTGGAGATAAAGATTTGGATGAATGGAAGGAAGCAGCTCGACGACTGAAGGCTTCTCAACCTGTCCACCCTGAAGATGGGAGAGTAGTGTTGAAATGTATAAAATTAAGCTATGATTACTTGAGATCTGATGATGCCAGATCATGCTTCTTACTTTGCTGCCTATTCCCAGAGGATTATGATATCCCAATTGAGGACTTGCTGGGGTATGGGTTCGGGAAAGGATTATTTCAACATTGCAACACATTGCAAGAAGCCAGAGCCACTGCTTATTCAGAGGTCAAGTACCTTAAAGCTTCTAGCTTGCTTTTGGCGGGTAAATATGACGAACAGGTTAGGATGCATGATGTCATTCGGGATATGGCCATACTAATTTCGTTCTCTGAAGACGGCCAACATTTTTTTGTCAAAGCTGGTTGTGAACTAAAGAATTGGCCAAAGATCAATACACAGAACGGCTACTCTGCAATCTCACTGATGTGGAATGAAATCCGCAAGCTACCAGAAGAGTTGGTATGTCCAAAACTTCAAATTTTATCAGTAAGGCATAATGTTGATTTAAATGAGATCCCAGAACCCTTTTTCTCATGTCCGAGTGCATTAAGGGTCTTAGATCTCAGTGACACTAGTATTTTTCTACTACCCCAATCATTTAGTCTCTTAACCAACCTCCAAGCTTTGTATTTAGATTTCTGCGAGAAAATAAGTGATATTTCCGTGCTTGGACAGCTTAAGAAGCTTGAGATTCTTAGTATGAAAGAATATCCTCTCAAAGAATTGTCGAGAGAAATAGGACGTTTGACCAATCTAAGGAAATTGGATGTCAGTTTTGATAGTTCCCGATTTGGAGGTATTGTCACAATTCCATCTGAAGTTATATCAAAGTTACGTAGATTGGAAGAACTGTACATGCTGTACTGTGGATTTTGGTATTGGGGGAGTAAAGTTGAGGGAGAAGAAGGAACTAATATTGGCTTTGATGAGATAACTAGCTTATCATACTTGAACATATTGACGGTTTGCATATCTGATGCAGAATGCATCCCTAAAACTGTTGAGTTGGATCCAAATTGGGTGTACTTTGATATATGTATCAGCAGAGGCAGAAGAATGGTACCTAGCCAGTCTCATTGTGGTCATAATTTAAGATCTTTGATTCTTGACAAACCCATCAATACCTTACCTTATTGGTTTGTCAACGTGGTGACAAAGAAATCGGAGAAGCTAGAGTATGAAGACTGCAGGGATTTGAATAACATTCTTGTGGAATATGACCATGGGATGTTACATGGACTGAAACATCTCTCTGTAAGTGGTCCTTGTAAGAAGTTGCAAGAGTTGATGAACACGATAACATGGGTTCCAAATAAACCTGTGTTCGAGAACTTGGAGGAGTTGCACCTGTATAGACTGGATTGCCTGAAGGAGTTATGTGTTGGTGAATTACTACCTGGGTCTCTAAGCAATTTGAAGGTATTATATGTCTCTGGTTGTTGTTATTTGGGGAATGTACTTTTACCATCAAAATTGTTACAGAGACTACCAAATCTGGAGAAACTAATATGTCATGCTATGGCTAGCATGAAATATGTGTTTGGATGTGAAGGGTTTGAGGCAGAACAATCAAATTTAAGAGAGATGAAGTTGTTGAATCTGGAGGCAGTAAGAAGCATATGTAATGTTCCAGCTCCACCTACAATGTTCCAGACTCTTAAAAGTTTGTTCATTCACAAATGCAAGTCTCTGCGATATATTTTCACATATGATGTAGCTCAATGTCTTTTTCAATTGGAAGACCTTTTTGTAGAGAGTTGCCCTAGCTTTGAAGGAGTATTAATTAAAGCAAGCAAGGAAACAATGAACAACAAGAAGATCGTTCTTCCAAAATTGAAGAACTTAGTTTTGGTGGATCTTCCTGTGCTGTGCAGTGACAGTGATAGTGATACTATTGATATCAACTGTCCTTCATTGAATCACGTTTATGTGAAGGACTGCCCCCTCTTTCCATTTTCCACCTTTGCTTCTGAATTCCTCAGCAGGAAACAATTCCAACTCAATGATGAACAGCATTTCTACTTTCTTCATAAGAGAATGCTGGAAGACTTTGGAGAAGACAAATAA

Protein Analysis

1095

Amino Acids

124.98

Weight (kDa)

5.98

Isoelectric Point (pI)

48.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 21 - 188 6.5e-49 TIR domain
TIR_2 PF13676 24 - 120 2.6e-14 TIR domain
NB-ARC PF00931 207 - 287 3.6e-11 NB-ARC domain
NB-ARC PF00931 312 - 356 1.4e-06 NB-ARC domain
LRR_8 PF13855 563 - 621 8.6e-07 Leucine rich repeat
LRR_14 PF23598 589 - 730 3e-13 Leucine-rich repeat region
LRR_RPS2 PF23247 852 - 994 1.8e-23 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 504
AasI GACNNNNNNGTC 1 cut(s) 182
Acc65I GGTACC 1 cut(s) 2281
AccB1I GGYRCC 1 cut(s) 2281
AccI GTMKAC 2 cut(s) 491, 1135
AciI CCGC 2 cut(s) 1481, 1660
AclWI GGATC 5 cut(s) 350, 1729, 2225, 2238, 3084
AcoI YGGCCR 5 cut(s) 471, 771, 1530, 1558, 1601
AcsI RAATTY 7 cut(s) 330, 804, 912, 1081, 1698, 2794, 3194
AcuI CTGAAG 6 cut(s) 781, 1235, 1260, 1572, 2058, 2609
AfaI GTAC 7 cut(s) 369, 1454, 2075, 2084, 2246, 2283, 2679
AfiI CCNNNNNNNGG 3 cut(s) 1244, 1360, 1977
AflII CTTAAG 1 cut(s) 1898
AflIII ACRYGT 1 cut(s) 721
AhlI ACTAGT 1 cut(s) 1793
AjiI CACGTC 1 cut(s) 352
AjnI CCWGG 1 cut(s) 2617
AjuI GAANNNNNNNTTGG 2 cut(s) 3053, 3085
AleI CACNNNNGTG 1 cut(s) 1645
Alw21I GWGCWC 1 cut(s) 733
Alw26I GTCTC 7 cut(s) 845, 1829, 2298, 2628, 2656, 2698, 2913
Alw44I GTGCAC 1 cut(s) 729
AlwI GGATC 5 cut(s) 350, 1729, 2225, 2238, 3084
AoxI GGCC 6 cut(s) 3, 471, 771, 1530, 1558, 1601
ApaLI GTGCAC 1 cut(s) 729
ApeKI GCWGC 5 cut(s) 29, 442, 527, 1202, 1326
ApoI RAATTY 7 cut(s) 330, 804, 912, 1081, 1698, 2794, 3194
AseI ATTAAT 1 cut(s) 3002
Asp700I GAANNNNTTC 1 cut(s) 3212
Asp718I GGTACC 1 cut(s) 2281
AspLEI GCGC 1 cut(s) 649
AspS9I GGNCC 1 cut(s) 2483
AsuHPI GGTGA 3 cut(s) 952, 2383, 2618
AsuII TTCGAA 1 cut(s) 117
AsuNHI GCTAGC 1 cut(s) 2744
AvaII GGWCC 1 cut(s) 2483
BaeGI GKGCMC 2 cut(s) 733, 1168
BalI TGGCCA 3 cut(s) 473, 1532, 1603
BamHI GGATCC 1 cut(s) 2230
BanI GGYRCC 1 cut(s) 2281
BbsI GAAGAC 4 cut(s) 1560, 2409, 2967, 3273
Bbv12I GWGCWC 1 cut(s) 733
BbvCI CCTCAGC 1 cut(s) 3200
BbvI GCAGC 5 cut(s) 41, 454, 539, 1214, 1313
BccI CCATC 4 cut(s) 1238, 2041, 2345, 2695
BceAI ACGGC 4 cut(s) 231, 758, 1573, 1639
BciT130I CCWGG 1 cut(s) 2619
BciVI GTATCC 1 cut(s) 790
BclI TGATCA 1 cut(s) 757
BcoDI GTCTC 7 cut(s) 845, 1829, 2298, 2628, 2656, 2698, 2913
BcuI ACTAGT 1 cut(s) 1793
BfmI CTRYAG 1 cut(s) 2407
BfoI RGCGCY 1 cut(s) 650
BfrI CTTAAG 1 cut(s) 1898
BfuI GTATCC 1 cut(s) 790
BglII AGATCT 4 cut(s) 823, 1292, 1780, 2315
BisI GCNGC 5 cut(s) 30, 443, 528, 1203, 1327
BlsI GCNGC 5 cut(s) 31, 444, 529, 1204, 1328
Bme1390I CCNGG 1 cut(s) 2619
Bme18I GGWCC 1 cut(s) 2483
BmgBI CACGTC 1 cut(s) 352
BmgT120I GGNCC 1 cut(s) 2483
BmiI GGNNCC 3 cut(s) 2232, 2283, 2529
BmrFI CCNGG 1 cut(s) 2619
BmrI ACTGGG 1 cut(s) 756
BmsI GCATC 7 cut(s) 41, 445, 1017, 1291, 1497, 2188, 2217
BmtI GCTAGC 1 cut(s) 2748
BmuI ACTGGG 1 cut(s) 756
BpiI GAAGAC 4 cut(s) 1560, 2409, 2967, 3273
BpmI CTGGAG 2 cut(s) 2738, 2843
Bpu10I CCTNAGC 1 cut(s) 3200
Bpu14I TTCGAA 1 cut(s) 117
BpuEI CTTGAG 2 cut(s) 1309, 1928
BsaAI YACGTR 1 cut(s) 2057
BsaI GGTCTC 1 cut(s) 2628
BsaJI CCNNGG 7 cut(s) 275, 588, 627, 711, 990, 2443, 2618
BsaXI ACNNNNNCTCC 4 cut(s) 986, 1016, 2007, 2037
Bsc4I CCNNNNNNNGG 3 cut(s) 1244, 1360, 1977
Bse1I ACTGG 5 cut(s) 751, 1000, 1139, 2290, 2583
Bse3DI GCAATG 3 cut(s) 452, 1402, 1412
BseBI CCWGG 1 cut(s) 2619
BseDI CCNNGG 7 cut(s) 275, 588, 627, 711, 990, 2443, 2618
BseGI GGATG 9 cut(s) 172, 196, 1008, 1192, 1512, 1993, 2208, 2454, 2771
BseLI CCNNNNNNNGG 3 cut(s) 1244, 1360, 1977
BseMI GCAATG 3 cut(s) 452, 1402, 1412
BseMII CTCAG 2 cut(s) 1798, 3214
BseNI ACTGG 5 cut(s) 751, 1000, 1139, 2290, 2583
BseRI GAGGAG 4 cut(s) 42, 397, 407, 2573
BseSI GKGCMC 2 cut(s) 733, 1168
BseXI GCAGC 5 cut(s) 41, 454, 539, 1214, 1313
BseYI CCCAGC 1 cut(s) 1369
BsgI GTGCAG 3 cut(s) 461, 568, 3112
BshFI GGCC 6 cut(s) 5, 473, 773, 1532, 1560, 1603
BshNI GGYRCC 1 cut(s) 2281
BsiHKAI GWGCWC 1 cut(s) 733
BslI CCNNNNNNNGG 3 cut(s) 1244, 1360, 1977
BsmAI GTCTC 7 cut(s) 845, 1829, 2298, 2628, 2656, 2698, 2913
BsmI GAATGC 2 cut(s) 2210, 3264
BsnI GGCC 6 cut(s) 5, 473, 773, 1532, 1560, 1603
Bso31I GGTCTC 1 cut(s) 2628
Bsp119I TTCGAA 1 cut(s) 117
Bsp1286I GDGCHC 2 cut(s) 733, 1168
Bsp1407I TGTACA 1 cut(s) 2073
Bsp19I CCATGG 5 cut(s) 275, 627, 711, 990, 2443
BspACI CCGC 2 cut(s) 1481, 1660
BspANI GGCC 6 cut(s) 5, 473, 773, 1532, 1560, 1603
BspCNI CTCAG 2 cut(s) 1797, 3213
BspLI GGNNCC 3 cut(s) 2232, 2283, 2529
BspMAI CTGCAG 1 cut(s) 2411
BspOI GCTAGC 1 cut(s) 2748
BspPI GGATC 5 cut(s) 350, 1729, 2225, 2238, 3084
BspQI GCTCTTC 1 cut(s) 859
BspT104I TTCGAA 1 cut(s) 117
BspT107I GGYRCC 1 cut(s) 2281
BspTI CTTAAG 1 cut(s) 1898
BspTNI GGTCTC 1 cut(s) 2628
BsrDI GCAATG 3 cut(s) 452, 1402, 1412
BsrGI TGTACA 1 cut(s) 2073
BsrI ACTGG 5 cut(s) 751, 1000, 1139, 2290, 2583
BssECI CCNNGG 7 cut(s) 275, 588, 627, 711, 990, 2443, 2618
BssNAI GTATAC 1 cut(s) 492
BssT1I CCWWGG 6 cut(s) 275, 588, 627, 711, 990, 2443
Bst1107I GTATAC 1 cut(s) 492
Bst2UI CCWGG 1 cut(s) 2619
Bst4CI ACNGT 7 cut(s) 670, 2073, 2087, 2185, 2221, 3101, 3128
Bst6I CTCTTC 4 cut(s) 374, 429, 859, 1668
BstAFI CTTAAG 1 cut(s) 1898
BstAUI TGTACA 1 cut(s) 2073
BstBAI YACGTR 1 cut(s) 2057
BstBI TTCGAA 1 cut(s) 117
BstC8I GCNNGC 5 cut(s) 739, 1472, 1664, 2746, 3013
BstDEI CTNAG 8 cut(s) 405, 1777, 1784, 1916, 1976, 2627, 3064, 3200
BstDSI CCRYGG 5 cut(s) 275, 627, 711, 990, 2443
BstF5I GGATG 9 cut(s) 172, 196, 1008, 1192, 1512, 1993, 2208, 2454, 2771
BstH2I RGCGCY 1 cut(s) 650
BstHHI GCGC 1 cut(s) 649
BstMAI GTCTC 7 cut(s) 845, 1829, 2298, 2628, 2656, 2698, 2913
BstMWI GCNNNNNNNGC 6 cut(s) 29, 611, 1005, 1151, 1894, 1903
BstNI CCWGG 1 cut(s) 2619
BstNSI RCATGY 2 cut(s) 725, 2080
BstSCI CCNGG 1 cut(s) 2617
BstSFI CTRYAG 1 cut(s) 2407
BstSLI GKGCMC 2 cut(s) 733, 1168
BstSNI TACGTA 1 cut(s) 2057
BstV1I GCAGC 5 cut(s) 41, 454, 539, 1214, 1313
BstV2I GAAGAC 4 cut(s) 1560, 2409, 2967, 3273
BstX2I RGATCY 7 cut(s) 823, 1292, 1734, 1780, 2230, 2315, 3076
BstYI RGATCY 7 cut(s) 823, 1292, 1734, 1780, 2230, 2315, 3076
BstZ17I GTATAC 1 cut(s) 492
BsuI GTATCC 1 cut(s) 790
BsuRI GGCC 6 cut(s) 5, 473, 773, 1532, 1560, 1603
BtgI CCRYGG 5 cut(s) 275, 627, 711, 990, 2443
BtrI CACGTC 1 cut(s) 352
BtsCI GGATG 9 cut(s) 172, 196, 1008, 1192, 1512, 1993, 2208, 2454, 2771
BtsI GCAGTG 2 cut(s) 1431, 3100
BtsIMutI CAGTG 6 cut(s) 666, 1431, 1640, 1792, 3100, 3106
Cac8I GCNNGC 5 cut(s) 739, 1472, 1664, 2746, 3013
CfoI GCGC 1 cut(s) 649
Cfr13I GGNCC 1 cut(s) 2483
CseI GACGC 1 cut(s) 948
Csp6I GTAC 7 cut(s) 368, 1453, 2074, 2083, 2245, 2282, 2678
CviQI GTAC 7 cut(s) 368, 1453, 2074, 2083, 2245, 2282, 2678
DdeI CTNAG 8 cut(s) 405, 1777, 1784, 1916, 1976, 2627, 3064, 3200
DraI TTTAAA 1 cut(s) 1728
DrdI GACNNNNNNGTC 1 cut(s) 182
DseDI GACNNNNNNGTC 1 cut(s) 182
EaeI YGGCCR 5 cut(s) 471, 771, 1530, 1558, 1601
Eam1104I CTCTTC 4 cut(s) 374, 429, 859, 1668
EarI CTCTTC 4 cut(s) 374, 429, 859, 1668
Eco105I TACGTA 1 cut(s) 2057
Eco130I CCWWGG 6 cut(s) 275, 588, 627, 711, 990, 2443
Eco31I GGTCTC 1 cut(s) 2628
Eco32I GATATC 3 cut(s) 374, 1351, 3121
Eco47I GGWCC 1 cut(s) 2483
Eco57I CTGAAG 6 cut(s) 781, 1235, 1260, 1572, 2058, 2609
EcoRI GAATTC 3 cut(s) 330, 912, 3194
EcoRII CCWGG 1 cut(s) 2617
EcoRV GATATC 3 cut(s) 374, 1351, 3121
EcoT14I CCWWGG 6 cut(s) 275, 588, 627, 711, 990, 2443
EcoT22I ATGCAT 3 cut(s) 314, 1512, 2210
ErhI CCWWGG 6 cut(s) 275, 588, 627, 711, 990, 2443
FalI AAGNNNNNCTT 4 cut(s) 1057, 1089, 2470, 2502
FauI CCCGC 1 cut(s) 1474
FauNDI CATATG 4 cut(s) 25, 607, 2840, 2929
FbaI TGATCA 1 cut(s) 757
FblI GTMKAC 2 cut(s) 491, 1135
Fnu4HI GCNGC 5 cut(s) 30, 443, 528, 1203, 1327
FokI GGATG 9 cut(s) 179, 203, 995, 1199, 1519, 2000, 2195, 2461, 2778
Fsp4HI GCNGC 5 cut(s) 30, 443, 528, 1203, 1327
GlaI GCGC 1 cut(s) 648
GluI GCNGC 5 cut(s) 30, 443, 528, 1203, 1327
GsaI CCCAGC 1 cut(s) 1373
GsuI CTGGAG 2 cut(s) 2738, 2843
HaeII RGCGCY 1 cut(s) 650
HaeIII GGCC 6 cut(s) 5, 473, 773, 1532, 1560, 1603
HgaI GACGC 1 cut(s) 948
HhaI GCGC 1 cut(s) 649
Hin6I GCGC 1 cut(s) 647
HinP1I GCGC 1 cut(s) 647
HincII GTYRAC 1 cut(s) 2365
HindII GTYRAC 1 cut(s) 2365
HindIII AAGCTT 3 cut(s) 1461, 1842, 1904
HinfI GANTC 9 cut(s) 227, 539, 678, 1037, 1912, 2323, 2818, 2875, 3139
HphI GGTGA 3 cut(s) 952, 2383, 2618
Hpy166II GTNNAC 8 cut(s) 492, 579, 731, 1136, 1234, 1589, 2245, 2365
Hpy8I GTNNAC 8 cut(s) 492, 579, 731, 1136, 1234, 1589, 2245, 2365
Hpy99I CGWCG 2 cut(s) 356, 1212
HpyCH4III ACNGT 7 cut(s) 670, 2073, 2087, 2185, 2221, 3101, 3128
HpyCH4IV ACGT 5 cut(s) 351, 1963, 2056, 2367, 3144
HpyF10VI GCNNNNNNNGC 6 cut(s) 29, 611, 1005, 1151, 1894, 1903
HpyF3I CTNAG 8 cut(s) 405, 1777, 1784, 1916, 1976, 2627, 3064, 3200
HpySE526I ACGT 5 cut(s) 351, 1963, 2056, 2367, 3144
HspAI GCGC 1 cut(s) 647
KpnI GGTACC 1 cut(s) 2285
Ksp22I TGATCA 1 cut(s) 757
LguI GCTCTTC 1 cut(s) 859
LmnI GCTCC 4 cut(s) 17, 55, 936, 2860
Lsp1109I GCAGC 5 cut(s) 41, 454, 539, 1214, 1313
LweI GCATC 7 cut(s) 41, 445, 1017, 1291, 1497, 2188, 2217
MaeII ACGT 5 cut(s) 351, 1963, 2056, 2367, 3144
MaeIII GTNAC 8 cut(s) 664, 1787, 2023, 2052, 2371, 2451, 2697, 3095
MfeI CAATTG 4 cut(s) 219, 240, 1356, 2954
MflI RGATCY 7 cut(s) 823, 1292, 1734, 1780, 2230, 2315, 3076
MhlI GDGCHC 2 cut(s) 733, 1168
MlsI TGGCCA 3 cut(s) 473, 1532, 1603
MluNI TGGCCA 3 cut(s) 473, 1532, 1603
MlyI GAGTC 2 cut(s) 687, 2869
MmeI TCCRAC 5 cut(s) 38, 810, 841, 2208, 3241
Mox20I TGGCCA 3 cut(s) 473, 1532, 1603
Mph1103I ATGCAT 3 cut(s) 314, 1512, 2210
MroXI GAANNNNTTC 1 cut(s) 3212
MscI TGGCCA 3 cut(s) 473, 1532, 1603
MslI CAYNNNNRTG 4 cut(s) 726, 1645, 2429, 3147
Msp20I TGGCCA 3 cut(s) 473, 1532, 1603
MspCI CTTAAG 1 cut(s) 1898
MspR9I CCNGG 1 cut(s) 2619
MunI CAATTG 4 cut(s) 219, 240, 1356, 2954
Mva1269I GAATGC 2 cut(s) 2210, 3264
MvaI CCWGG 1 cut(s) 2619
MwoI GCNNNNNNNGC 6 cut(s) 29, 611, 1005, 1151, 1894, 1903
NcoI CCATGG 5 cut(s) 275, 627, 711, 990, 2443
NdeI CATATG 4 cut(s) 25, 607, 2840, 2929
NheI GCTAGC 1 cut(s) 2744
NlaIV GGNNCC 3 cut(s) 2232, 2283, 2529
NmuCI GTSAC 5 cut(s) 664, 1787, 2023, 2371, 3095
NsiI ATGCAT 3 cut(s) 314, 1512, 2210
NspI RCATGY 2 cut(s) 725, 2080
NspV TTCGAA 1 cut(s) 117
OliI CACNNNNGTG 1 cut(s) 1645
PacI TTAATTAA 1 cut(s) 3006
PciI ACATGT 1 cut(s) 721
PciSI GCTCTTC 1 cut(s) 859
PctI GAATGC 2 cut(s) 2210, 3264
PdmI GAANNNNTTC 1 cut(s) 3212
PfeI GAWTC 7 cut(s) 227, 539, 1037, 1912, 2323, 2818, 3139
PkrI GCNGC 5 cut(s) 31, 444, 529, 1204, 1328
PleI GAGTC 2 cut(s) 686, 2869
PpsI GAGTC 2 cut(s) 686, 2869
Ppu21I YACGTR 1 cut(s) 2057
PscI ACATGT 1 cut(s) 721
PshBI ATTAAT 1 cut(s) 3002
PsiI TTATAA 1 cut(s) 504
Psp6I CCWGG 1 cut(s) 2617
PspFI CCCAGC 1 cut(s) 1369
PspGI CCWGG 1 cut(s) 2617
PspN4I GGNNCC 3 cut(s) 2232, 2283, 2529
PspPI GGNCC 1 cut(s) 2483
PstI CTGCAG 1 cut(s) 2411
PsuI RGATCY 7 cut(s) 823, 1292, 1734, 1780, 2230, 2315, 3076
RsaI GTAC 7 cut(s) 369, 1454, 2075, 2084, 2246, 2283, 2679
RsaNI GTAC 7 cut(s) 368, 1453, 2074, 2083, 2245, 2282, 2678
RseI CAYNNNNRTG 4 cut(s) 726, 1645, 2429, 3147
SapI GCTCTTC 1 cut(s) 859
SatI GCNGC 5 cut(s) 30, 443, 528, 1203, 1327
Sau96I GGNCC 1 cut(s) 2483
SchI GAGTC 2 cut(s) 687, 2869
ScrFI CCNGG 1 cut(s) 2619
SduI GDGCHC 2 cut(s) 733, 1168
SfaNI GCATC 7 cut(s) 41, 445, 1017, 1291, 1497, 2188, 2217
SfcI CTRYAG 1 cut(s) 2407
SfuI TTCGAA 1 cut(s) 117
SinI GGWCC 1 cut(s) 2483
SmiI ATTTAAAT 1 cut(s) 1728
SmiMI CAYNNNNRTG 4 cut(s) 726, 1645, 2429, 3147
SmlI CTYRAG 4 cut(s) 7, 1288, 1898, 1907
SmoI CTYRAG 4 cut(s) 7, 1288, 1898, 1907
SnaBI TACGTA 1 cut(s) 2057
SpeI ACTAGT 1 cut(s) 1793
SsiI CCGC 2 cut(s) 1481, 1660
SspI AATATT 1 cut(s) 2137
StyD4I CCNGG 1 cut(s) 2617
StyI CCWWGG 6 cut(s) 275, 588, 627, 711, 990, 2443
SwaI ATTTAAAT 1 cut(s) 1728
TaaI ACNGT 7 cut(s) 670, 2073, 2087, 2185, 2221, 3101, 3128
TaiI ACGT 5 cut(s) 354, 1966, 2059, 2370, 3147
TaqI TCGA 7 cut(s) 117, 130, 354, 1085, 1207, 1949, 2547
TatI WGTACW 4 cut(s) 2073, 2082, 2244, 2677
TfiI GAWTC 7 cut(s) 227, 539, 1037, 1912, 2323, 2818, 3139
TscAI CASTG 6 cut(s) 673, 1438, 1647, 1792, 3100, 3106
TseFI GTSAC 5 cut(s) 664, 1787, 2023, 2371, 3095
TseI GCWGC 5 cut(s) 29, 442, 527, 1202, 1326
Tsp45I GTSAC 5 cut(s) 664, 1787, 2023, 2371, 3095
TspGWI ACGGA 1 cut(s) 1873
TspRI CASTG 6 cut(s) 673, 1438, 1647, 1792, 3100, 3106
Vha464I CTTAAG 1 cut(s) 1898
VneI GTGCAC 1 cut(s) 729
VpaK11BI GGWCC 1 cut(s) 2483
VspI ATTAAT 1 cut(s) 3002
XapI RAATTY 7 cut(s) 330, 804, 912, 1081, 1698, 2794, 3194
XceI RCATGY 2 cut(s) 725, 2080
XcmI CCANNNNNNNNNTGG 2 cut(s) 1241, 2297
XmiI GTMKAC 2 cut(s) 491, 1135
XmnI GAANNNNTTC 1 cut(s) 3212
Zsp2I ATGCAT 3 cut(s) 314, 1512, 2210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.