RchiOBHm_Chr3g0483491

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000085
Physical Location & Seq
Forward (+)
29745406 .. 29753830
8425 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44824

Sequence Viewer

Length: 3501 bp
ATGGCCTCGAGCTCCCAAACAAGCTCTTCATCTCTTCCTTCGTCATCAGCTCCTCAGTGGAAGCATGATGTGTTTCTCAGCTTCAGGGGTGAAGACACCCGGAAGGGTATTGTATCCTCTTTATACCATGAATTGAAAACCAGAGGAATACAAACATTCATGGATGACCAAGGGCTTCAGCAAGGGATGCCTATTTCTCCAAGTCTCCTAACAGCGATTGAGGAATCCAGGTGTGCAGTCGTTCTTCTATCGCCAAACTATGCTTCTTCTACATGGTGTTTGGATGAACTTGCAAAGATTCTCCAATGCTCTGAAGCCAAGAAGACACTGCTTCTGCCAATTTTTTATAATGTGGATCCCTCTCATGTGCGACATCAAAAGGGAAGTTTTGCAGAAGCCTTCACTAAGCATGAAGAGAGGTATAGGCAAGGCAATGATGATGCAGAGAAGGTGAACAAGTGGAGAGCTGCTTTAACACAAGTGGCCAATCTCTCTGGGTTGGATTCAAACAAATTTGGGTCTGACAGAGAACTTGTCCAACGCATTGTGGAACTTGTGCGCCGTGATGTAAGGCCTATTTCAATAATGTCGAGCAGCAATTTTGAAGCATTTGAATCAACAAGAAAAGCCATGAAGGAGCTGATGGAGGCATTAGAAGATGATGAGGTCGCTGCCATTGGGGTCTATGGAATGGGAGGGGTTGGGAAGACAACCATGGTGAAACATGTCGGTGCACAAGCCCAAAAAATGGGGCTTTTTGATCGTGTGATTATGGCTGTTGTATCCCAAACCCCAGATACAAGAAATATTCAAGGCGCACTTGCGGATCTGCTGGGCTTGACATTGGTGCAGGAGTCAGATATTGGACGAGCCGATAGATTGCAGACGGAGATACTTGGAAGAAAAAAGATCCTTATAATCTTAGACGACATTTGGAAGACACTTGACTTGTCAAGCATTGGAATTCCCAGCTACAACTACCTTCAAATCCGCAAGTCCAAACTGCTATTCACCACAAGGAGATCGAATGTTTGCCATACCATGGGGAGCCAAGCAAAAATTCATCTCAATATCTTATCAGAAGATGATGCTTGGAACTTGTTTTTGAAGAAGGCAAGAAGGTCTTTTCAAAAGTCTAGCCCTTTCTATGAAGTAGCCAGAAAGGTAGCTAGAGAATGTGCTGGTCTCCCAGTTGCATTGATTGCAGTTGCGAGGGCACTTGGAGATAAAGATTTTAAAGACTGGAAACTAGCAGCTCGACAACTTGAGATGTCTCAACCTGCCAACCTTGACGAGGAGAAAGATGTGTTCAAGTGCATTAAGTTGAGCTATGATTACCTTGACAGTGACGACGCCAAGTCTTGCTTCTTGCTTTGCTGCCTATTCCCAGAAGATTATGACATCCGTGTTGAAGACTTGATGAGCTATGGGTTCGGAACAGGGTTGTTTCGACATGTCAACAGCACAATAGAATATGCCAGAGTCAGAGCACATTCGGTTACTAGATACCTTAAAGCTTCTTGTCTGCTTTTGGATGGGACAAAAGAAGGATATGTAAGGATGCATGATGTCATTCGAGATATGGCCATATCCATTGCACTCTCTGTAGATGGCCACGCCTCTTTGGTGAAAACTAATTGTCATTTGAAAGATTGGTCGATGGATGCGGACGAAGGCTACTCAGCAATCTCACTAATCGAGAGCAACACTTGCAAGCTTCCCAACAAGTTGGTATGTTCAAAACTTCAGATTTTATTGCTACAAAAAAATGATGGTATAAGAGAGATCTCAAAAACCTTTTTTCAAAGCCCCAATGATTTAAGGGTCTTAGATCTTAGGAAAACTGGCATTTCATCACTGCCCCCATCATTCAGTCTCCTAGCCAGACTCCAAGCTTTGCATTTAGATGATTGCGAGTCCATCATTGACATATCCCTACTTGGAAAACTGAAAAAGCTTGAGATTCTTAGTATGAGACACTGTGACTCTATTAAGAAATTTCCAAAAGAGATTGGAAATTTGACGAATTTAAGAATGTTGGACTTGACTGGAACCCGTTTTAAGACAGTTCCCTCTCAAGTGATATCAAGGTTGTATAGATTGGAAGTACTGTACATGAGATGTTATTTTGGCAAGTGGGGTTGTAAAGCTGCCGGAGGCTGCTTGTGTTTTCTTTGTAAGAATGCTAGCTTTGATGAGTTAATTGGCTTGCCAAGTTTAAACACTTTGCAGGTTTCCGTATGTGGTGCAGAACGCTTGCCTCAAAACGTTGAGGTCAATCCAAATTGGGACAGGTTTTGTATAATTATGGAAGGATACGGCACGGACATTTGTCGCATTTATGATTATGACGAGGGCTTCCCACATCGTTTTTCAAGAACATTGATCACGGTCGAAACCATGAATAACTTACCCGATTGGTTCATCAGTGTAGTGACAGAAAGAACAGATTATTTATGGTACAGAGGTCGCCATGGCTTAACTAACCTTCTCGAGGAACACGACTGTGGGAGATTACATGGACTCAAGTATTTGTGTATAGAATCTTCCGAATATGTGGAAGTGTTGTTGAACACAATAACATGGACTAGGGTGTCAAATGAACCTGTGTTTGAGAACTTGGAAGAGTTGGAATTCTCCGGGGATGGCTTGGAGGAGCTATGTGTCGGTGAATTACCACCTGGCTCTCTACGTAATCTAAAGAAATTGATGATGGGTATGTGTCCTTGCTTGGTGAAACCGCTTTTACCGTCAAAGTTGTTGCACAGATTAGAGAGTCTGGAAGAACTATGTTGTTCTCTTATGAAGGAAATGAAATATGTGTTTGGATTTGAAGGGCATGAACCACAACACGTTGTCTTGACGAGGCTGAGAATGATTTCATTGGAATCCATGGACAGCTTAATAAACATATGGGATGGTCCTGCTCCATCTGGAATCTTCCAGAGTCTCAAAATCATTTCCTTGGTTAATTGCCGGAAACTGGAATATCTCTTCACATCTGATGTAGCTCGATGTCTTTTGCAATTGGAAGACGTTGTGGTACAGAATTGCTTTAGCTTGGACAGAATAATTGAAGCGAGTGAGGAAATAGAGAGCAACAGGATCGTTCTTCCACAGTTGAAGAACTTGGTTTTAAAAGATCTTCCAAAGCTCACAAGGCTGTCCAGTACAAGCAGCAGTACTATTGATATTGAAATCGAGTGCCCTACATTGGAACGCTTATATGTCTCTGCGTGCCCCCAGTTATCAATCCCTGCTTCTGGTTTCAACAGCAGCAACGAACACTCACTATTTAATTGGGGTTATAGCGATACACTACGGAGTAGTATCGAGGCCGTGAGTTACCGCTCACACTTTCCACCTTCGATGAGTGCGCGCTTACTTTTGGCACTTCTGCGGACAACACTGATTTCGACCAAGAGGGCCCCAGTGACGTGGCCAGTTGCATGTTTTGCCGTCATGGTCTTGTGTTTAATGGTATTTTGGCATGTGGGATGA

Protein Analysis

1166

Amino Acids

131.52

Weight (kDa)

6.52

Isoelectric Point (pI)

47.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 21 - 198 1.4e-52 TIR domain
TIR_2 PF13676 24 - 121 6.7e-17 TIR domain
NB-ARC PF00931 209 - 373 3.6e-32 NB-ARC domain
LRR_8 PF13855 581 - 638 1.4e-07 Leucine rich repeat
LRR_14 PF23598 606 - 662 1.9e-06 Leucine-rich repeat region
LRR_14 PF23598 633 - 707 7.8e-10 Leucine-rich repeat region
LRR_RPS2 PF23247 874 - 1018 1.1e-23 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 348, 917
AasI GACNNNNNNGTC 1 cut(s) 2593
Acc36I ACCTGC 2 cut(s) 1288, 2221
AccB7I CCANNNNNTGG 2 cut(s) 748, 1042
AccBSI CCGCTC 1 cut(s) 3351
AccII CGCG 1 cut(s) 3379
AciI CCGC 6 cut(s) 824, 991, 1667, 2741, 3349, 3400
AclI AACGTT 1 cut(s) 2268
AclWI GGATC 5 cut(s) 350, 363, 834, 904, 3113
AcoI YGGCCR 4 cut(s) 483, 1584, 1612, 3440
AcsI RAATTY 7 cut(s) 512, 963, 1059, 1997, 2017, 2026, 2633
AcuI CTGAAG 4 cut(s) 67, 161, 333, 1730
AcyI GRCGYC 1 cut(s) 1353
AfaI GTAC 6 cut(s) 2109, 2114, 2462, 3045, 3172, 3184
AfiI CCNNNNNNNGG 7 cut(s) 748, 1042, 1294, 2494, 2983, 3214, 3263
AflIII ACRYGT 3 cut(s) 724, 1453, 2851
AhdI GACNNNNNGTC 1 cut(s) 1357
AjiI CACGTC 1 cut(s) 3438
AjnI CCWGG 2 cut(s) 227, 2680
AjuI GAANNNNNNNTTGG 8 cut(s) 247, 279, 992, 1024, 1044, 1076, 1349, 1381
AleI CACNNNNGTG 1 cut(s) 2505
Alw21I GWGCWC 3 cut(s) 14, 736, 1492
Alw26I GTCTC 7 cut(s) 209, 1190, 1278, 1880, 1969, 2954, 3235
Alw44I GTGCAC 1 cut(s) 732
AlwI GGATC 5 cut(s) 350, 363, 834, 904, 3113
Ama87I CYCGRG 2 cut(s) 7, 2492
AoxI GGCC 8 cut(s) 3, 483, 572, 1584, 1612, 3336, 3426, 3440
ApaI GGGCCC 1 cut(s) 3430
ApaLI GTGCAC 1 cut(s) 732
ApeKI GCWGC 9 cut(s) 467, 594, 671, 1253, 1377, 2150, 2160, 3177, 3276
ApoI RAATTY 7 cut(s) 512, 963, 1059, 1997, 2017, 2026, 2633
Asp700I GAANNNNTTC 1 cut(s) 2878
AspLEI GCGC 4 cut(s) 561, 818, 3379, 3381
AspS9I GGNCC 3 cut(s) 2921, 3426, 3427
AsuC2I CCSGG 2 cut(s) 100, 2641
AsuHPI GGTGA 7 cut(s) 101, 463, 730, 1003, 1639, 2681, 2746
AsuNHI GCTAGC 1 cut(s) 2186
AvaI CYCGRG 2 cut(s) 7, 2492
AvaII GGWCC 1 cut(s) 2921
BaeGI GKGCMC 5 cut(s) 736, 1219, 3209, 3242, 3430
BaeI ACNNNNGTAYC 2 cut(s) 2452, 2485
BalI TGGCCA 4 cut(s) 485, 1586, 1614, 3442
BamHI GGATCC 1 cut(s) 355
BanII GRGCYC 2 cut(s) 14, 3430
BarI GAAGNNNNNNTAC 2 cut(s) 2530, 2562
BbsI GAAGAC 6 cut(s) 99, 329, 713, 944, 1419, 3039
Bbv12I GWGCWC 3 cut(s) 14, 736, 1492
BbvI GCAGC 9 cut(s) 454, 606, 658, 1265, 1364, 2137, 2147, 3189, 3288
BceAI ACGGC 4 cut(s) 546, 2335, 3323, 3443
BcgI CGANNNNNNTGC 2 cut(s) 3088, 3122
BciT130I CCWGG 2 cut(s) 229, 2682
BciVI GTATCC 3 cut(s) 124, 793, 2309
BclI TGATCA 1 cut(s) 2385
BcnI CCSGG 2 cut(s) 100, 2641
BcoDI GTCTC 7 cut(s) 209, 1190, 1278, 1880, 1969, 2954, 3235
BfaI CTAG 7 cut(s) 1137, 1170, 1250, 1503, 1880, 2187, 2589
BfmI CTRYAG 1 cut(s) 1605
BfuAI ACCTGC 2 cut(s) 1288, 2221
BfuI GTATCC 3 cut(s) 124, 793, 2309
BglII AGATCT 3 cut(s) 1785, 1831, 3142
BisI GCNGC 9 cut(s) 468, 595, 672, 1254, 1378, 2151, 2161, 3178, 3277
BlsI GCNGC 9 cut(s) 469, 596, 673, 1255, 1379, 2152, 2162, 3179, 3278
BmcAI AGTACT 2 cut(s) 2109, 3184
Bme1390I CCNGG 4 cut(s) 100, 229, 2641, 2682
Bme18I GGWCC 1 cut(s) 2921
BmeRI GACNNNNNGTC 1 cut(s) 1357
BmeT110I CYCGRG 2 cut(s) 7, 2492
BmgBI CACGTC 1 cut(s) 3438
BmgT120I GGNCC 3 cut(s) 2921, 3426, 3427
BmiI GGNNCC 5 cut(s) 357, 1049, 2053, 3428, 3429
BmrFI CCNGG 4 cut(s) 100, 229, 2641, 2682
BmrI ACTGGG 3 cut(s) 1184, 3238, 3425
BmsI GCATC 5 cut(s) 177, 430, 1078, 1551, 1654
BmtI GCTAGC 1 cut(s) 2190
BmuI ACTGGG 3 cut(s) 1184, 3238, 3425
BoxI GACNNNNGTC 1 cut(s) 2331
BpiI GAAGAC 6 cut(s) 99, 329, 713, 944, 1419, 3039
BpuEI CTTGAG 4 cut(s) 1286, 1979, 2061, 2510
BpuMI CCSGG 2 cut(s) 100, 2641
BsaAI YACGTR 1 cut(s) 2693
BsaHI GRCGYC 1 cut(s) 1353
BsaI GGTCTC 1 cut(s) 1190
BsaJI CCNNGG 7 cut(s) 169, 714, 1041, 2473, 2640, 2892, 2964
Bsc4I CCNNNNNNNGG 7 cut(s) 748, 1042, 1294, 2494, 2983, 3214, 3263
Bse1I ACTGG 9 cut(s) 1190, 1247, 1849, 2053, 2988, 3168, 3244, 3431, 3443
Bse3DI GCAATG 2 cut(s) 439, 1593
BseBI CCWGG 2 cut(s) 229, 2682
BseDI CCNNGG 7 cut(s) 169, 714, 1041, 2473, 2640, 2892, 2964
BseGI GGATG 9 cut(s) 169, 192, 289, 1401, 1540, 1566, 1669, 2650, 2923
BseLI CCNNNNNNNGG 7 cut(s) 748, 1042, 1294, 2494, 2983, 3214, 3263
BseMI GCAATG 2 cut(s) 439, 1593
BseMII CTCAG 4 cut(s) 68, 91, 1695, 2861
BseNI ACTGG 9 cut(s) 1190, 1247, 1849, 2053, 2988, 3168, 3244, 3431, 3443
BsePI GCGCGC 1 cut(s) 3377
BseRI GAGGAG 3 cut(s) 42, 1310, 2669
BseSI GKGCMC 5 cut(s) 736, 1219, 3209, 3242, 3430
BseXI GCAGC 9 cut(s) 454, 606, 658, 1265, 1364, 2137, 2147, 3189, 3288
BseYI CCCAGC 2 cut(s) 832, 968
BsgI GTGCAG 3 cut(s) 255, 869, 2268
Bsh1236I CGCG 1 cut(s) 3379
Bsh1285I CGRYCG 1 cut(s) 2394
BshFI GGCC 8 cut(s) 5, 485, 574, 1586, 1614, 3338, 3428, 3442
BsiEI CGRYCG 1 cut(s) 2394
BsiHKAI GWGCWC 3 cut(s) 14, 736, 1492
BsiHKCI CYCGRG 2 cut(s) 7, 2492
BsiSI CCGG 4 cut(s) 100, 2154, 2640, 2977
BslFI GGGAC 2 cut(s) 1552, 2303
BslI CCNNNNNNNGG 7 cut(s) 748, 1042, 1294, 2494, 2983, 3214, 3263
BsmAI GTCTC 7 cut(s) 209, 1190, 1278, 1880, 1969, 2954, 3235
BsmFI GGGAC 2 cut(s) 1552, 2303
BsmI GAATGC 1 cut(s) 2188
BsnI GGCC 8 cut(s) 5, 485, 574, 1586, 1614, 3338, 3428, 3442
Bso31I GGTCTC 1 cut(s) 1190
BsoBI CYCGRG 2 cut(s) 7, 2492
Bsp120I GGGCCC 1 cut(s) 3426
Bsp1286I GDGCHC 7 cut(s) 14, 736, 1219, 1492, 3209, 3242, 3430
Bsp1407I TGTACA 1 cut(s) 2112
Bsp19I CCATGG 4 cut(s) 714, 1041, 2473, 2892
BspACI CCGC 6 cut(s) 824, 991, 1667, 2741, 3349, 3400
BspANI GGCC 8 cut(s) 5, 485, 574, 1586, 1614, 3338, 3428, 3442
BspCNI CTCAG 4 cut(s) 67, 90, 1694, 2862
BspFNI CGCG 1 cut(s) 3379
BspLI GGNNCC 5 cut(s) 357, 1049, 2053, 3428, 3429
BspMI ACCTGC 2 cut(s) 1288, 2221
BspOI GCTAGC 1 cut(s) 2190
BspPI GGATC 5 cut(s) 350, 363, 834, 904, 3113
BspQI GCTCTTC 1 cut(s) 31
BspTNI GGTCTC 1 cut(s) 1190
BsrBI CCGCTC 1 cut(s) 3351
BsrDI GCAATG 2 cut(s) 439, 1593
BsrGI TGTACA 1 cut(s) 2112
BsrI ACTGG 9 cut(s) 1190, 1247, 1849, 2053, 2988, 3168, 3244, 3431, 3443
BssECI CCNNGG 7 cut(s) 169, 714, 1041, 2473, 2640, 2892, 2964
BssHII GCGCGC 1 cut(s) 3377
BssNI GRCGYC 1 cut(s) 1353
BssT1I CCWWGG 6 cut(s) 169, 714, 1041, 2473, 2892, 2964
Bst2UI CCWGG 2 cut(s) 229, 2682
Bst4CI ACNGT 8 cut(s) 1346, 1982, 2068, 2112, 2392, 2507, 2751, 3120
Bst6I CTCTTC 5 cut(s) 31, 39, 408, 2619, 2999
BstACI GRCGYC 1 cut(s) 1353
BstAPI GCANNNNNTGC 4 cut(s) 187, 1202, 1710, 3455
BstAUI TGTACA 1 cut(s) 2112
BstBAI YACGTR 1 cut(s) 2693
BstC8I GCNNGC 6 cut(s) 1715, 2188, 2210, 2258, 3238, 3379
BstDEI CTNAG 9 cut(s) 54, 77, 405, 922, 1681, 1828, 1835, 1967, 2870
BstDSI CCRYGG 4 cut(s) 714, 1041, 2473, 2892
BstENI CCTNNNNNAGG 2 cut(s) 1292, 2492
BstF5I GGATG 9 cut(s) 169, 192, 289, 1401, 1540, 1566, 1669, 2650, 2923
BstFNI CGCG 1 cut(s) 3379
BstHHI GCGC 4 cut(s) 561, 818, 3379, 3381
BstMAI GTCTC 7 cut(s) 209, 1190, 1278, 1880, 1969, 2954, 3235
BstMCI CGRYCG 1 cut(s) 2394
BstMWI GCNNNNNNNGC 5 cut(s) 187, 1202, 1710, 3160, 3455
BstNI CCWGG 2 cut(s) 229, 2682
BstNSI RCATGY 4 cut(s) 728, 1457, 3453, 3494
BstPAI GACNNNNGTC 1 cut(s) 2331
BstSCI CCNGG 4 cut(s) 98, 227, 2639, 2680
BstSFI CTRYAG 1 cut(s) 1605
BstSLI GKGCMC 5 cut(s) 736, 1219, 3209, 3242, 3430
BstSNI TACGTA 1 cut(s) 2693
BstUI CGCG 1 cut(s) 3379
BstV1I GCAGC 9 cut(s) 454, 606, 658, 1265, 1364, 2137, 2147, 3189, 3288
BstV2I GAAGAC 6 cut(s) 99, 329, 713, 944, 1419, 3039
BstX2I RGATCY 6 cut(s) 355, 826, 909, 1785, 1831, 3142
BstXI CCANNNNNNTGG 2 cut(s) 1729, 3438
BstYI RGATCY 6 cut(s) 355, 826, 909, 1785, 1831, 3142
BsuI GTATCC 3 cut(s) 124, 793, 2309
BsuRI GGCC 8 cut(s) 5, 485, 574, 1586, 1614, 3338, 3428, 3442
BtgI CCRYGG 4 cut(s) 714, 1041, 2473, 2892
BtrI CACGTC 1 cut(s) 3438
BtsCI GGATG 9 cut(s) 169, 192, 289, 1401, 1540, 1566, 1669, 2650, 2923
BtsI GCAGTG 2 cut(s) 326, 1856
BtsIMutI CAGTG 8 cut(s) 62, 326, 1351, 1856, 1978, 2434, 3407, 3438
BveI ACCTGC 2 cut(s) 1288, 2221
Cac8I GCNNGC 6 cut(s) 1715, 2188, 2210, 2258, 3238, 3379
CfoI GCGC 4 cut(s) 561, 818, 3379, 3381
Cfr13I GGNCC 3 cut(s) 2921, 3426, 3427
CseI GACGC 1 cut(s) 1361
Csp6I GTAC 6 cut(s) 2108, 2113, 2461, 3044, 3171, 3183
CviQI GTAC 6 cut(s) 2108, 2113, 2461, 3044, 3171, 3183
DdeI CTNAG 9 cut(s) 54, 77, 405, 922, 1681, 1828, 1835, 1967, 2870
DraI TTTAAA 3 cut(s) 1237, 2220, 3138
DrdI GACNNNNNNGTC 1 cut(s) 2593
DriI GACNNNNNGTC 1 cut(s) 1357
DseDI GACNNNNNNGTC 1 cut(s) 2593
EaeI YGGCCR 4 cut(s) 483, 1584, 1612, 3440
Eam1104I CTCTTC 5 cut(s) 31, 39, 408, 2619, 2999
Eam1105I GACNNNNNGTC 1 cut(s) 1357
EarI CTCTTC 5 cut(s) 31, 39, 408, 2619, 2999
Ecl136II GAGCTC 1 cut(s) 12
Eco105I TACGTA 1 cut(s) 2693
Eco130I CCWWGG 6 cut(s) 169, 714, 1041, 2473, 2892, 2964
Eco147I AGGCCT 1 cut(s) 574
Eco24I GRGCYC 2 cut(s) 14, 3430
Eco31I GGTCTC 1 cut(s) 1190
Eco32I GATATC 1 cut(s) 2085
Eco47I GGWCC 1 cut(s) 2921
Eco53kI GAGCTC 1 cut(s) 12
Eco57I CTGAAG 4 cut(s) 67, 161, 333, 1730
Eco88I CYCGRG 2 cut(s) 7, 2492
EcoICRI GAGCTC 1 cut(s) 12
EcoNI CCTNNNNNAGG 2 cut(s) 1292, 2492
EcoO109I RGGNCCY 2 cut(s) 3426, 3427
EcoRI GAATTC 2 cut(s) 963, 2633
EcoRII CCWGG 2 cut(s) 227, 2680
EcoRV GATATC 1 cut(s) 2085
EcoT14I CCWWGG 6 cut(s) 169, 714, 1041, 2473, 2892, 2964
EcoT22I ATGCAT 1 cut(s) 1566
EcoT38I GRGCYC 2 cut(s) 14, 3430
ErhI CCWWGG 6 cut(s) 169, 714, 1041, 2473, 2892, 2964
FalI AAGNNNNNCTT 6 cut(s) 804, 836, 1108, 1140, 1349, 1381
FaqI GGGAC 2 cut(s) 1552, 2303
FauNDI CATATG 1 cut(s) 2912
FbaI TGATCA 1 cut(s) 2385
Fnu4HI GCNGC 9 cut(s) 468, 595, 672, 1254, 1378, 2151, 2161, 3178, 3277
FokI GGATG 9 cut(s) 176, 199, 296, 1388, 1547, 1573, 1676, 2657, 2930
FriOI GRGCYC 2 cut(s) 14, 3430
Fsp4HI GCNGC 9 cut(s) 468, 595, 672, 1254, 1378, 2151, 2161, 3178, 3277
FspBI CTAG 7 cut(s) 1137, 1170, 1250, 1503, 1880, 2187, 2589
GlaI GCGC 4 cut(s) 560, 817, 3378, 3380
GluI GCNGC 9 cut(s) 468, 595, 672, 1254, 1378, 2151, 2161, 3178, 3277
GsaI CCCAGC 2 cut(s) 836, 972
HaeIII GGCC 8 cut(s) 5, 485, 574, 1586, 1614, 3338, 3428, 3442
HapII CCGG 4 cut(s) 100, 2154, 2640, 2977
HgaI GACGC 1 cut(s) 1361
HhaI GCGC 4 cut(s) 561, 818, 3379, 3381
Hin1I GRCGYC 1 cut(s) 1353
Hin6I GCGC 4 cut(s) 559, 816, 3377, 3379
HinP1I GCGC 4 cut(s) 559, 816, 3377, 3379
HincII GTYRAC 1 cut(s) 1459
HindII GTYRAC 1 cut(s) 1459
HindIII AAGCTT 4 cut(s) 1515, 1715, 1893, 1955
HpaII CCGG 4 cut(s) 100, 2154, 2640, 2977
HphI GGTGA 7 cut(s) 101, 463, 730, 1003, 1639, 2681, 2746
Hpy166II GTNNAC 3 cut(s) 454, 734, 1459
Hpy188I TCNGA 9 cut(s) 313, 523, 859, 1081, 1436, 1487, 1749, 2551, 3004
Hpy188III TCNNGA 8 cut(s) 1577, 1699, 2376, 2492, 2780, 2860, 2934, 2944
Hpy8I GTNNAC 3 cut(s) 454, 734, 1459
Hpy99I CGWCG 1 cut(s) 1355
HpyCH4III ACNGT 8 cut(s) 1346, 1982, 2068, 2112, 2392, 2507, 2751, 3120
HpyCH4IV ACGT 5 cut(s) 2268, 2692, 2853, 3036, 3437
HpyF10VI GCNNNNNNNGC 5 cut(s) 187, 1202, 1710, 3160, 3455
HpyF3I CTNAG 9 cut(s) 54, 77, 405, 922, 1681, 1828, 1835, 1967, 2870
HpySE526I ACGT 5 cut(s) 2268, 2692, 2853, 3036, 3437
Hsp92I GRCGYC 1 cut(s) 1353
HspAI GCGC 4 cut(s) 559, 816, 3377, 3379
Ksp22I TGATCA 1 cut(s) 2385
LguI GCTCTTC 1 cut(s) 31
LmnI GCTCC 6 cut(s) 17, 55, 637, 1047, 2656, 2932
Lsp1109I GCAGC 9 cut(s) 454, 606, 658, 1265, 1364, 2137, 2147, 3189, 3288
LweI GCATC 5 cut(s) 177, 430, 1078, 1551, 1654
MaeI CTAG 7 cut(s) 1137, 1170, 1250, 1503, 1880, 2187, 2589
MaeII ACGT 5 cut(s) 2268, 2692, 2853, 3036, 3437
MaeIII GTNAC 6 cut(s) 1346, 1498, 1982, 2434, 3344, 3433
MbiI CCGCTC 1 cut(s) 3351
MfeI CAATTG 1 cut(s) 3026
MflI RGATCY 6 cut(s) 355, 826, 909, 1785, 1831, 3142
MhlI GDGCHC 7 cut(s) 14, 736, 1219, 1492, 3209, 3242, 3430
MlsI TGGCCA 4 cut(s) 485, 1586, 1614, 3442
MluNI TGGCCA 4 cut(s) 485, 1586, 1614, 3442
MlyI GAGTC 8 cut(s) 863, 1491, 1881, 1925, 1979, 2517, 2785, 2956
MmeI TCCRAC 4 cut(s) 480, 562, 2019, 2610
Mox20I TGGCCA 4 cut(s) 485, 1586, 1614, 3442
Mph1103I ATGCAT 1 cut(s) 1566
MroXI GAANNNNTTC 1 cut(s) 2878
MscI TGGCCA 4 cut(s) 485, 1586, 1614, 3442
MslI CAYNNNNRTG 3 cut(s) 729, 1905, 2505
Msp20I TGGCCA 4 cut(s) 485, 1586, 1614, 3442
MspI CCGG 4 cut(s) 100, 2154, 2640, 2977
MspR9I CCNGG 4 cut(s) 100, 229, 2641, 2682
MssI GTTTAAAC 1 cut(s) 2220
MunI CAATTG 1 cut(s) 3026
Mva1269I GAATGC 1 cut(s) 2188
MvaI CCWGG 2 cut(s) 229, 2682
MvnI CGCG 1 cut(s) 3379
MwoI GCNNNNNNNGC 5 cut(s) 187, 1202, 1710, 3160, 3455
NciI CCSGG 2 cut(s) 100, 2641
NcoI CCATGG 4 cut(s) 714, 1041, 2473, 2892
NdeI CATATG 1 cut(s) 2912
NheI GCTAGC 1 cut(s) 2186
NlaIV GGNNCC 5 cut(s) 357, 1049, 2053, 3428, 3429
NmuCI GTSAC 4 cut(s) 1346, 1982, 2434, 3433
NsiI ATGCAT 1 cut(s) 1566
NspI RCATGY 4 cut(s) 728, 1457, 3453, 3494
OliI CACNNNNGTG 1 cut(s) 2505
PaeR7I CTCGAG 2 cut(s) 7, 2492
PauI GCGCGC 1 cut(s) 3377
PceI AGGCCT 1 cut(s) 574
PciI ACATGT 2 cut(s) 724, 1453
PciSI GCTCTTC 1 cut(s) 31
PcsI WCGNNNNNNNCGW 1 cut(s) 2499
PctI GAATGC 1 cut(s) 2188
PdmI GAANNNNTTC 1 cut(s) 2878
PfeI GAWTC 8 cut(s) 224, 298, 503, 614, 1963, 2543, 2888, 2937
PflMI CCANNNNNTGG 2 cut(s) 748, 1042
PkrI GCNGC 9 cut(s) 469, 596, 673, 1255, 1379, 2152, 2162, 3179, 3278
PleI GAGTC 8 cut(s) 862, 1490, 1881, 1924, 1979, 2517, 2784, 2955
PmeI GTTTAAAC 1 cut(s) 2220
PpsI GAGTC 8 cut(s) 862, 1490, 1881, 1924, 1979, 2517, 2784, 2955
Ppu21I YACGTR 1 cut(s) 2693
PscI ACATGT 2 cut(s) 724, 1453
PshAI GACNNNNGTC 1 cut(s) 2331
PsiI TTATAA 2 cut(s) 348, 917
Psp124BI GAGCTC 1 cut(s) 14
Psp1406I AACGTT 1 cut(s) 2268
Psp6I CCWGG 2 cut(s) 227, 2680
PspFI CCCAGC 2 cut(s) 832, 968
PspGI CCWGG 2 cut(s) 227, 2680
PspN4I GGNNCC 5 cut(s) 357, 1049, 2053, 3428, 3429
PspOMI GGGCCC 1 cut(s) 3426
PspPI GGNCC 3 cut(s) 2921, 3426, 3427
PspXI VCTCGAGB 1 cut(s) 7
PsuI RGATCY 6 cut(s) 355, 826, 909, 1785, 1831, 3142
PteI GCGCGC 1 cut(s) 3377
RsaI GTAC 6 cut(s) 2109, 2114, 2462, 3045, 3172, 3184
RsaNI GTAC 6 cut(s) 2108, 2113, 2461, 3044, 3171, 3183
RseI CAYNNNNRTG 3 cut(s) 729, 1905, 2505
SacI GAGCTC 1 cut(s) 14
SapI GCTCTTC 1 cut(s) 31
SatI GCNGC 9 cut(s) 468, 595, 672, 1254, 1378, 2151, 2161, 3178, 3277
Sau96I GGNCC 3 cut(s) 2921, 3426, 3427
ScaI AGTACT 2 cut(s) 2109, 3184
SchI GAGTC 8 cut(s) 863, 1491, 1881, 1925, 1979, 2517, 2785, 2956
ScrFI CCNGG 4 cut(s) 100, 229, 2641, 2682
SduI GDGCHC 7 cut(s) 14, 736, 1219, 1492, 3209, 3242, 3430
SfaNI GCATC 5 cut(s) 177, 430, 1078, 1551, 1654
SfcI CTRYAG 1 cut(s) 1605
Sfr274I CTCGAG 2 cut(s) 7, 2492
SinI GGWCC 1 cut(s) 2921
SlaI CTCGAG 2 cut(s) 7, 2492
SmiMI CAYNNNNRTG 3 cut(s) 729, 1905, 2505
SmlI CTYRAG 6 cut(s) 7, 1265, 1958, 2076, 2492, 2525
SmoI CTYRAG 6 cut(s) 7, 1265, 1958, 2076, 2492, 2525
SnaBI TACGTA 1 cut(s) 2693
SseBI AGGCCT 1 cut(s) 574
SsiI CCGC 6 cut(s) 824, 991, 1667, 2741, 3349, 3400
SspI AATATT 1 cut(s) 808
SspMI CTAG 7 cut(s) 1137, 1170, 1250, 1503, 1880, 2187, 2589
SstI GAGCTC 1 cut(s) 14
StuI AGGCCT 1 cut(s) 574
StyD4I CCNGG 4 cut(s) 98, 227, 2639, 2680
StyI CCWWGG 6 cut(s) 169, 714, 1041, 2473, 2892, 2964
TaaI ACNGT 8 cut(s) 1346, 1982, 2068, 2112, 2392, 2507, 2751, 3120
TaiI ACGT 5 cut(s) 2271, 2695, 2856, 3039, 3440
TatI WGTACW 4 cut(s) 2107, 2112, 3170, 3182
TfiI GAWTC 8 cut(s) 224, 298, 503, 614, 1963, 2543, 2888, 2937
TscAI CASTG 8 cut(s) 62, 333, 1351, 1863, 1985, 2434, 3414, 3438
TseFI GTSAC 4 cut(s) 1346, 1982, 2434, 3433
TseI GCWGC 9 cut(s) 467, 594, 671, 1253, 1377, 2150, 2160, 3177, 3276
Tsp45I GTSAC 4 cut(s) 1346, 1982, 2434, 3433
TspGWI ACGGA 5 cut(s) 902, 1394, 2227, 2339, 3337
TspRI CASTG 8 cut(s) 62, 333, 1351, 1863, 1985, 2434, 3414, 3438
Van91I CCANNNNNTGG 2 cut(s) 748, 1042
VneI GTGCAC 1 cut(s) 732
VpaK11BI GGWCC 1 cut(s) 2921
XagI CCTNNNNNAGG 2 cut(s) 1292, 2492
XapI RAATTY 7 cut(s) 512, 963, 1059, 1997, 2017, 2026, 2633
XceI RCATGY 4 cut(s) 728, 1457, 3453, 3494
XhoI CTCGAG 2 cut(s) 7, 2492
XmnI GAANNNNTTC 1 cut(s) 2878
XspI CTAG 7 cut(s) 1137, 1170, 1250, 1503, 1880, 2187, 2589
ZrmI AGTACT 2 cut(s) 2109, 3184
Zsp2I ATGCAT 1 cut(s) 1566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.