Rw3G015980

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000085
Physical Location & Seq
Reverse (-)
16548533 .. 16553782
5250 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G015980.1

Sequence Viewer

Length: 3516 bp
ATGGAGTACTATTTCTTCGAGACTGTGTTGGCGATTATTGTGGTAATTGTGACATGGATCGGTGTCATAGCCAGTCTTAAGTGGATATGGAAGAGTTGGTTCAATTACATGGTTGCAACCCCACAGCCAATATCAGAGGGAAATCAAGAACCAGTTGACGTTGATTCCTCTGCATCTCTTCCTTCATCATCAGCTGAATCATCAGCTTCTAGGTGGAAGCATGATGTGTTTTTGAGTTTCAGGGGTGCAGACACTCGAAAAGGTATCGCATCAGAAATATACTATCGACTGCAAAACAAGAGAGGAATCAAAACATTCATGGATGACCAAGACCTTCAAGTAGGGGATGTTATTTCTCCCACTCTCCTAACGGCAATTAAAGAATCAAGGTTTGCAATTATTGTTCTCTCTCAAAACTATGCCTCTTCCACTTGGTGTTTGGAGGAACTTAGAAACGTTTGTGAATGCAAGGAAGAAGACAACAACAGAATTCTGCCACTTTTTTATTATGTTGATCCTACTGATGTACGATATCAGACGAGGAGTTTCAGAGATGCTTTTACTAGGCATGAAACATCAGGGCAACACAGATCAGAGAAGGTGCAGCAGTGGAGAGATGCTTTAAAAAAAGTGGCAAATTTCTCTGGATGGCACACACAGAATTATAAAACTGAAAAAGAACTTTTGGATGCCATTGAGGAATCTGTGTGCAATAAATTACGACCTACTGGACATGAGTTTAAATTGTCCCTCGGAGGTTTTGAAGCATTTGCAGCAACAAAGCAAGCCATTGATAAGGTCATGAATGCGCTAAAAGATGATGAGGCCACTACCATTGGAGTCTACGGAATGGGGGGCGTTGGAAAGACAACAATGGTGAAATATGTTGGAGCACAAGCCCAAAAAAGTAGGCTTTTTAATCAAGTGATTATGGCTGTCGTATCCCAAAACCCCGACTTGATGAAAATTCAAGAGACATTTGCAGAACTGCTGGGCTTTAAATTGGAGGAGAAGACAGAAATTGGAAGAGCCATTAAATTGAAGGAGAAGATAATGAGAGGAACCGGGATCCTCATAATCTTAGATGACATTTGGAAGAGAATAGACTTTTCAAGCATAGGAATTCCGAGCCACAACGAACTCCAAAGATGCAATTCCAAAGTTCTACTGACCACCAGAAAATTAAGTGTTTGCCGTTCCATGGATTGCCATCCAAACATACATCTCAATATCTTATCAGAAGAAGATTCTTGGAGCTTATTTGTGAAGGAAGCAAGGAAGTCTTTTCACAAATCATCCGATTTCTATTATGTAGCACGTAAGGTGGCTAGAGAATGCGCTGGTCTACCAATTGCTTTGATAACAGTTGCGAGGGCCCTTCGAGATGAAGGTTTGGATGGATGGAAAGAAGCTGCTCGAAGACTAAAAGCGTCCCAACCTGCCAAACATGAAGATGAGTCAGATGTGTTTGCATGCATAAAGTTAAGCTATGATTACTTGAAATCTGATGATTCCAAATCATGCTTCTTGCTATGCTGCTTGTTCCCAGAAGATTATGATATCCCAATTAAATTCTTGTTCAAGTATGGAATTGGGAAAGGAATGTTTCAAGATTCAAACTTGCAAGAAGCCCGAGCCACAACATATTCGGTGGTGAAGGCCCTTATAGATTCTAGCTTGCTTTTGGACGCTAGAGATGACAGATATGTAAGGATGCATGATGTCATTCGGGATATGGCAATCTTAATTTCGTTATCTGAAGGCCAGCGGTTCTTGGTGAAAGCTGGCTGTGAATTGAAGAATTGGCCACATATTGATGCACATAAAGGCTACTATGGAATCTCACTAATGCAGAACAAAATTTGCAAGCTGCCCCAAGAGTTGGTATGTCCAAATCTCCAGATTTTATTACTGCAACACAATGCTTCTATAAATGAGATCCCAAAATCTTTTCTCCAAGGTCAGAATGAATTAAGAGTCTTGGATCTTAGCGACACTGGTATTTCATTACTACCCCAATCAATCAGTTTCTTAACCAACCTTCAAGCTTTGTATTTAGATTACTGCTGGAACATAATTGACATTTCTGTAATCGGAACACTTCACAAGCTTGAGATTCTTAGTATGAGAGGAACTGCTCTGGAAGAATTGTCAAGAGAAATAGGACAGTTGACCAATCTAAAGATGCTGGATGTCAGTGCTAGTAGATTCTCGGGTGGACGTATTTGCACAATTCCATCTAAAGTGGTATCAAAGTTGCATAAATTAGAAGAACTGTACATGCAATACTGTGGATTTTGGGACTGGGGGAGTAAAATTGATGGAGAAGGAGAAGAAACTAATATTGGCTTTGATGAATTAGCTGATTTATCATATTTAAGCATTTTGAAAGTTTGCATACCCGATGCACATTGCATCCCTAGAAGTGTTGAAGTCGAACCGAATTGGGTTTACTTCGATATTTTTATTTGCACCAATAGACGCGGTCTTCGTAGGAGTATTACGAACTTACAATCTGGTCATAAGTGTAGGTCCTTGAGACTTTATAGAACTGACGCAACCATAGGAACCTTTCCGGACTGGTTTGTCAACGCTGGGGTAAAGAATACTGAGAGGCTACTTTATGAAAAATGCGAAGGGTTGAGTAACATCCTTGTGGAGTATGACCGTGGGAGGTTACATGGACTGAAATTCCTCTCTGTGTCTGGTCCCTGTGAGAACTTGAAAGAATTGATGAATGCAATAACATGTGTTCCACGTATGCCTGTGTTTGAGAACTTGGAAGAGCTGTATCTGAAAAACCTGGATGACCTGAAGCAGTTATGTGTTGGTGAGTTACCACCTCGATCTCTCCGCAGTCTTAAATTACTGATGGTCCTTGAGTGTCGTAACTTGGGGAATGTACTGTTGCCATCAAAATTGTTGCAGAAACTACCAAATCTGGAAAAGCTAACGTGTTGGAATAGTAAAGTGAAATATGTGTTTGGATGTGGAGGATTTGAGCCAGATCAAACAAATTTGAGAGAGATGGACTTATGGAATCTACACGTAGTAAGAAGCATATGTAATGGTCCTGCTCCACGTGGAATGTTCCAGGCTCTAAAGGAATTGGTCATTTATAATTGCAACTTCCAGGGAAGCCTCTTCACATTTGATGTAGCTCAGTGTCTTTTTCAATTGGAAAAACTTATCGTATACGTATGCCCTGTCTTGGAAAGAGTAATCGAAGAAAGGAGGGAAACATTGAACAACAAGAAGACCGTTCTTCCAAAATTGAAGAACTTATCGTTGCTTAGTCTTCCAATGTTGTACAAGGGAAGTGCTACTATTGATTTTGAGTGTCCTTCATTGGAAACCTTGAGTCTGTGGAACTGCCCCCACTTGTCATTTCCATCCTCTGCTTCTGACTACTTCCACAGCAGGAACCAAGTCCAAGTCCAAGTCCAATTCATCTCAATTCTAAAGTACGGGAAAATTTTAGGATATATCCCCAATAAAGTGAAGAAATATCTATAA

Protein Analysis

1171

Amino Acids

133.52

Weight (kDa)

7.99

Isoelectric Point (pI)

44.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 73 - 243 2.6e-50 TIR domain
TIR_2 PF13676 76 - 187 1.9e-17 TIR domain
NB-ARC PF00931 261 - 426 1.6e-30 NB-ARC domain
LRR_8 PF13855 631 - 689 4.1e-08 Leucine rich repeat
LRR_14 PF23598 635 - 764 8.5e-13 Leucine-rich repeat region
LRR_RPS2 PF23247 927 - 1068 3.9e-19 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1008 - 1134 1.4e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 666, 3120
AasI GACNNNNNNGTC 1 cut(s) 2585
Acc36I ACCTGC 1 cut(s) 1447
AccB7I CCANNNNNTGG 1 cut(s) 1882
AccI GTMKAC 3 cut(s) 843, 1345, 3195
AccII CGCG 1 cut(s) 2484
AccIII TCCGGA 1 cut(s) 2575
AciI CCGC 3 cut(s) 1768, 2484, 2854
AclI AACGTT 1 cut(s) 456
AclWI GGATC 6 cut(s) 65, 509, 1063, 1076, 1933, 1992
AcoI YGGCCR 1 cut(s) 1805
AcsI RAATTY 9 cut(s) 489, 637, 966, 1122, 1571, 1860, 2690, 3016, 3474
AcuI CTGAAG 2 cut(s) 1779, 2834
AcvI CACGTG 1 cut(s) 3083
AdeI CACNNNGTG 1 cut(s) 435
AfaI GTAC 6 cut(s) 8, 528, 2279, 2904, 3311, 3467
AfiI CCNNNNNNNGG 3 cut(s) 1201, 1882, 3211
AflII CTTAAG 1 cut(s) 77
AflIII ACRYGT 3 cut(s) 2747, 2954, 3046
AjnI CCWGG 3 cut(s) 2802, 3093, 3132
AjuI GAANNNNNNNTTGG 4 cut(s) 1508, 1540, 2328, 2360
Alw21I GWGCWC 1 cut(s) 895
Alw26I GTCTC 3 cut(s) 14, 968, 2533
AlwI GGATC 6 cut(s) 65, 509, 1063, 1076, 1933, 1992
Ama87I CYCGRG 2 cut(s) 1632, 2212
Aor13HI TCCGGA 1 cut(s) 2575
AoxI GGCC 5 cut(s) 825, 1374, 1659, 1762, 1805
ApaI GGGCCC 1 cut(s) 1378
ApeKI GCWGC 5 cut(s) 604, 773, 1412, 1536, 1870
ApoI RAATTY 9 cut(s) 489, 637, 966, 1122, 1571, 1860, 2690, 3016, 3474
ArsI GACNNNNNNTTYG 2 cut(s) 2472, 2504
AspLEI GCGC 2 cut(s) 811, 1340
AspS9I GGNCC 7 cut(s) 1374, 1375, 1660, 2532, 2708, 2875, 3071
AsuC2I CCSGG 1 cut(s) 1066
AsuHPI GGTGA 4 cut(s) 889, 1666, 1789, 2843
AvaI CYCGRG 2 cut(s) 1632, 2212
AvaII GGWCC 4 cut(s) 2532, 2708, 2875, 3071
BaeGI GKGCMC 1 cut(s) 1378
BalI TGGCCA 1 cut(s) 1807
BamHI GGATCC 1 cut(s) 1068
BanII GRGCYC 1 cut(s) 1378
BarI GAAGNNNNNNTAC 1 cut(s) 31
BbrPI CACGTG 1 cut(s) 3083
BbsI GAAGAC 6 cut(s) 483, 1019, 1426, 2480, 3263, 3290
Bbv12I GWGCWC 1 cut(s) 895
BbvI GCAGC 5 cut(s) 616, 785, 1399, 1523, 1857
BceAI ACGGC 2 cut(s) 387, 1179
BciT130I CCWGG 3 cut(s) 2804, 3095, 3134
BciVI GTATCC 1 cut(s) 952
BcnI CCSGG 1 cut(s) 1066
BcoDI GTCTC 3 cut(s) 14, 968, 2533
BfaI CTAG 7 cut(s) 210, 564, 1329, 1674, 1692, 2202, 2421
BfrI CTTAAG 1 cut(s) 77
BfuAI ACCTGC 1 cut(s) 1447
BfuI GTATCC 1 cut(s) 952
BisI GCNGC 5 cut(s) 605, 774, 1413, 1537, 1871
BlsI GCNGC 5 cut(s) 606, 775, 1414, 1538, 1872
BmcAI AGTACT 1 cut(s) 8
Bme1390I CCNGG 4 cut(s) 1066, 2804, 3095, 3134
Bme18I GGWCC 4 cut(s) 2532, 2708, 2875, 3071
BmeT110I CYCGRG 2 cut(s) 1632, 2212
BmgT120I GGNCC 7 cut(s) 1374, 1375, 1660, 2532, 2708, 2875, 3071
BmiI GGNNCC 6 cut(s) 1063, 1070, 1376, 2569, 2710, 3425
BmrFI CCNGG 4 cut(s) 1066, 2804, 3095, 3134
BmrI ACTGGG 1 cut(s) 2314
BmuI ACTGGG 1 cut(s) 2314
BpiI GAAGAC 6 cut(s) 483, 1019, 1426, 2480, 3263, 3290
BpmI CTGGAG 1 cut(s) 1883
BpuEI CTTGAG 4 cut(s) 2132, 2557, 2900, 3379
BpuMI CCSGG 1 cut(s) 1066
BsaAI YACGTR 5 cut(s) 1319, 2759, 3049, 3083, 3199
BsaBI GATNNNNATC 1 cut(s) 1209
BsaJI CCNNGG 5 cut(s) 751, 1200, 1957, 2668, 3133
BsaWI WCCGGW 1 cut(s) 2575
Bsc4I CCNNNNNNNGG 3 cut(s) 1201, 1882, 3211
Bse1I ACTGG 6 cut(s) 72, 152, 733, 2002, 2309, 2585
Bse3DI GCAATG 1 cut(s) 2410
Bse8I GATNNNNATC 1 cut(s) 1209
BseAI TCCGGA 1 cut(s) 2575
BseBI CCWGG 3 cut(s) 2804, 3095, 3134
BseDI CCNNGG 5 cut(s) 751, 1200, 1957, 2668, 3133
BseJI GATNNNNATC 1 cut(s) 1209
BseLI CCNNNNNNNGG 3 cut(s) 1201, 1882, 3211
BseMI GCAATG 1 cut(s) 2410
BseMII CTCAG 2 cut(s) 2601, 3176
BseNI ACTGG 6 cut(s) 72, 152, 733, 2002, 2309, 2585
BseRI GAGGAG 2 cut(s) 556, 1022
BseSI GKGCMC 1 cut(s) 1378
BseXI GCAGC 5 cut(s) 616, 785, 1399, 1523, 1857
BseYI CCCAGC 2 cut(s) 991, 2594
BsgI GTGCAG 2 cut(s) 267, 623
Bsh1236I CGCG 1 cut(s) 2484
BshFI GGCC 5 cut(s) 827, 1376, 1661, 1764, 1807
BsiHKAI GWGCWC 1 cut(s) 895
BsiHKCI CYCGRG 2 cut(s) 1632, 2212
BsiSI CCGG 2 cut(s) 1065, 2576
BslFI GGGAC 4 cut(s) 733, 1417, 2315, 2694
BslI CCNNNNNNNGG 3 cut(s) 1201, 1882, 3211
BsmAI GTCTC 3 cut(s) 14, 968, 2533
BsmFI GGGAC 4 cut(s) 733, 1417, 2315, 2694
BsmI GAATGC 4 cut(s) 470, 811, 1340, 2743
BsnI GGCC 5 cut(s) 827, 1376, 1661, 1764, 1807
BsoBI CYCGRG 2 cut(s) 1632, 2212
Bsp120I GGGCCC 1 cut(s) 1374
Bsp1286I GDGCHC 2 cut(s) 895, 1378
Bsp13I TCCGGA 1 cut(s) 2575
Bsp1407I TGTACA 2 cut(s) 2277, 3309
Bsp143I GATC 8 cut(s) 57, 514, 590, 1068, 1938, 1984, 2846, 3007
Bsp19I CCATGG 1 cut(s) 1200
BspACI CCGC 3 cut(s) 1768, 2484, 2854
BspANI GGCC 5 cut(s) 827, 1376, 1661, 1764, 1807
BspCNI CTCAG 2 cut(s) 2602, 3175
BspEI TCCGGA 1 cut(s) 2575
BspFNI CGCG 1 cut(s) 2484
BspHI TCATGA 1 cut(s) 801
BspLI GGNNCC 6 cut(s) 1063, 1070, 1376, 2569, 2710, 3425
BspMI ACCTGC 1 cut(s) 1447
BspPI GGATC 6 cut(s) 65, 509, 1063, 1076, 1933, 1992
BspQI GCTCTTC 2 cut(s) 1021, 2778
BspTI CTTAAG 1 cut(s) 77
BsrDI GCAATG 1 cut(s) 2410
BsrGI TGTACA 2 cut(s) 2277, 3309
BsrI ACTGG 6 cut(s) 72, 152, 733, 2002, 2309, 2585
BssECI CCNNGG 5 cut(s) 751, 1200, 1957, 2668, 3133
BssMI GATC 8 cut(s) 57, 514, 590, 1068, 1938, 1984, 2846, 3007
BssNAI GTATAC 1 cut(s) 3196
BssT1I CCWWGG 2 cut(s) 1200, 1957
Bst1107I GTATAC 1 cut(s) 3196
Bst2UI CCWGG 3 cut(s) 2804, 3095, 3134
Bst4CI ACNGT 8 cut(s) 25, 1366, 2169, 2277, 2291, 2669, 2907, 3262
Bst6I CTCTTC 7 cut(s) 86, 183, 430, 1021, 1091, 2778, 3149
BstAFI CTTAAG 1 cut(s) 77
BstAUI TGTACA 2 cut(s) 2277, 3309
BstBAI YACGTR 5 cut(s) 1319, 2759, 3049, 3083, 3199
BstC8I GCNNGC 6 cut(s) 786, 1474, 1679, 1766, 1786, 1868
BstDEI CTNAG 7 cut(s) 449, 1081, 1988, 2120, 2610, 3162, 3293
BstDSI CCRYGG 2 cut(s) 1200, 2668
BstFNI CGCG 1 cut(s) 2484
BstHHI GCGC 2 cut(s) 811, 1340
BstKTI GATC 8 cut(s) 60, 517, 593, 1071, 1941, 1987, 2849, 3010
BstMAI GTCTC 3 cut(s) 14, 968, 2533
BstMBI GATC 8 cut(s) 57, 514, 590, 1068, 1938, 1984, 2846, 3007
BstMWI GCNNNNNNNGC 1 cut(s) 773
BstNI CCWGG 3 cut(s) 2804, 3095, 3134
BstNSI RCATGY 3 cut(s) 1476, 2284, 2751
BstSCI CCNGG 4 cut(s) 1064, 2802, 3093, 3132
BstSLI GKGCMC 1 cut(s) 1378
BstSNI TACGTA 1 cut(s) 3199
BstUI CGCG 1 cut(s) 2484
BstV1I GCAGC 5 cut(s) 616, 785, 1399, 1523, 1857
BstV2I GAAGAC 6 cut(s) 483, 1019, 1426, 2480, 3263, 3290
BstX2I RGATCY 3 cut(s) 1068, 1938, 1984
BstYI RGATCY 3 cut(s) 1068, 1938, 1984
BstZ17I GTATAC 1 cut(s) 3196
BsuI GTATCC 1 cut(s) 952
BsuRI GGCC 5 cut(s) 827, 1376, 1661, 1764, 1807
BtgI CCRYGG 2 cut(s) 1200, 2668
BtsI GCAGTG 1 cut(s) 614
BtsIMutI CAGTG 4 cut(s) 614, 1995, 2203, 3170
BveI ACCTGC 1 cut(s) 1447
Cac8I GCNNGC 6 cut(s) 786, 1474, 1679, 1766, 1786, 1868
CciI TCATGA 1 cut(s) 801
CfoI GCGC 2 cut(s) 811, 1340
Cfr13I GGNCC 7 cut(s) 1374, 1375, 1660, 2532, 2708, 2875, 3071
CseI GACGC 4 cut(s) 1419, 1697, 2490, 2564
Csp6I GTAC 6 cut(s) 7, 527, 2278, 2903, 3310, 3466
CspCI CAANNNNNGTGG 2 cut(s) 817, 852
CviQI GTAC 6 cut(s) 7, 527, 2278, 2903, 3310, 3466
DdeI CTNAG 7 cut(s) 449, 1081, 1988, 2120, 2610, 3162, 3293
DpnI GATC 8 cut(s) 59, 516, 592, 1070, 1940, 1986, 2848, 3009
DpnII GATC 8 cut(s) 57, 514, 590, 1068, 1938, 1984, 2846, 3007
DraI TTTAAA 3 cut(s) 624, 742, 1000
DraIII CACNNNGTG 1 cut(s) 435
DrdI GACNNNNNNGTC 1 cut(s) 2585
DseDI GACNNNNNNGTC 1 cut(s) 2585
EaeI YGGCCR 1 cut(s) 1805
Eam1104I CTCTTC 7 cut(s) 86, 183, 430, 1021, 1091, 2778, 3149
EarI CTCTTC 7 cut(s) 86, 183, 430, 1021, 1091, 2778, 3149
Eco105I TACGTA 1 cut(s) 3199
Eco130I CCWWGG 2 cut(s) 1200, 1957
Eco24I GRGCYC 1 cut(s) 1378
Eco32I GATATC 2 cut(s) 533, 1561
Eco47I GGWCC 4 cut(s) 2532, 2708, 2875, 3071
Eco57I CTGAAG 2 cut(s) 1779, 2834
Eco72I CACGTG 1 cut(s) 3083
Eco88I CYCGRG 2 cut(s) 1632, 2212
EcoO109I RGGNCCY 4 cut(s) 1374, 1375, 1660, 2532
EcoRI GAATTC 2 cut(s) 489, 1122
EcoRII CCWGG 3 cut(s) 2802, 3093, 3132
EcoRV GATATC 2 cut(s) 533, 1561
EcoT14I CCWWGG 2 cut(s) 1200, 1957
EcoT22I ATGCAT 2 cut(s) 1478, 1719
EcoT38I GRGCYC 1 cut(s) 1378
ErhI CCWWGG 2 cut(s) 1200, 1957
FalI AAGNNNNNCTT 2 cut(s) 1267, 1299
FaqI GGGAC 4 cut(s) 733, 1417, 2315, 2694
FauNDI CATATG 1 cut(s) 3062
FblI GTMKAC 3 cut(s) 843, 1345, 3195
Fnu4HI GCNGC 5 cut(s) 605, 774, 1413, 1537, 1871
FriOI GRGCYC 1 cut(s) 1378
Fsp4HI GCNGC 5 cut(s) 605, 774, 1413, 1537, 1871
FspBI CTAG 7 cut(s) 210, 564, 1329, 1674, 1692, 2202, 2421
GlaI GCGC 2 cut(s) 810, 1339
GluI GCNGC 5 cut(s) 605, 774, 1413, 1537, 1871
GsaI CCCAGC 2 cut(s) 995, 2598
GsuI CTGGAG 1 cut(s) 1883
HaeIII GGCC 5 cut(s) 827, 1376, 1661, 1764, 1807
HapII CCGG 2 cut(s) 1065, 2576
HgaI GACGC 4 cut(s) 1419, 1697, 2490, 2564
HhaI GCGC 2 cut(s) 811, 1340
Hin6I GCGC 2 cut(s) 809, 1338
HinP1I GCGC 2 cut(s) 809, 1338
HincII GTYRAC 3 cut(s) 157, 2172, 2590
HindII GTYRAC 3 cut(s) 157, 2172, 2590
HindIII AAGCTT 2 cut(s) 2046, 2108
HpaII CCGG 2 cut(s) 1065, 2576
HphI GGTGA 4 cut(s) 889, 1666, 1789, 2843
Hpy166II GTNNAC 8 cut(s) 157, 844, 1346, 2172, 2219, 2452, 2590, 3196
Hpy8I GTNNAC 8 cut(s) 157, 844, 1346, 2172, 2219, 2452, 2590, 3196
HpyCH4III ACNGT 8 cut(s) 25, 1366, 2169, 2277, 2291, 2669, 2907, 3262
HpyCH4IV ACGT 9 cut(s) 159, 456, 1318, 2221, 2758, 2954, 3048, 3082, 3198
HpyF10VI GCNNNNNNNGC 1 cut(s) 773
HpyF3I CTNAG 7 cut(s) 449, 1081, 1988, 2120, 2610, 3162, 3293
HpySE526I ACGT 9 cut(s) 159, 456, 1318, 2221, 2758, 2954, 3048, 3082, 3198
HspAI GCGC 2 cut(s) 809, 1338
Kpn2I TCCGGA 1 cut(s) 2575
Kzo9I GATC 8 cut(s) 57, 514, 590, 1068, 1938, 1984, 2846, 3007
LguI GCTCTTC 2 cut(s) 1021, 2778
LmnI GCTCC 3 cut(s) 890, 1254, 3082
Lsp1109I GCAGC 5 cut(s) 616, 785, 1399, 1523, 1857
MaeI CTAG 7 cut(s) 210, 564, 1329, 1674, 1692, 2202, 2421
MaeII ACGT 9 cut(s) 159, 456, 1318, 2221, 2758, 2954, 3048, 3082, 3198
MaeIII GTNAC 5 cut(s) 49, 2645, 2676, 2835, 2888
MalI GATC 8 cut(s) 59, 516, 592, 1070, 1940, 1986, 2848, 3009
MboI GATC 8 cut(s) 57, 514, 590, 1068, 1938, 1984, 2846, 3007
MfeI CAATTG 2 cut(s) 1350, 3176
MflI RGATCY 3 cut(s) 1068, 1938, 1984
MhlI GDGCHC 2 cut(s) 895, 1378
MlsI TGGCCA 1 cut(s) 1807
MluNI TGGCCA 1 cut(s) 1807
MlyI GAGTC 4 cut(s) 849, 1466, 1986, 3370
MmeI TCCRAC 3 cut(s) 841, 868, 2939
Mox20I TGGCCA 1 cut(s) 1807
Mph1103I ATGCAT 2 cut(s) 1478, 1719
MroI TCCGGA 1 cut(s) 2575
MscI TGGCCA 1 cut(s) 1807
MslI CAYNNNNRTG 3 cut(s) 1452, 1815, 2654
Msp20I TGGCCA 1 cut(s) 1807
MspA1I CMGCKG 2 cut(s) 194, 1768
MspCI CTTAAG 1 cut(s) 77
MspI CCGG 2 cut(s) 1065, 2576
MspR9I CCNGG 4 cut(s) 1066, 2804, 3095, 3134
MunI CAATTG 2 cut(s) 1350, 3176
Mva1269I GAATGC 4 cut(s) 470, 811, 1340, 2743
MvaI CCWGG 3 cut(s) 2804, 3095, 3134
MvnI CGCG 1 cut(s) 2484
MwoI GCNNNNNNNGC 1 cut(s) 773
NciI CCSGG 1 cut(s) 1066
NcoI CCATGG 1 cut(s) 1200
NdeI CATATG 1 cut(s) 3062
NdeII GATC 8 cut(s) 57, 514, 590, 1068, 1938, 1984, 2846, 3007
NlaIV GGNNCC 6 cut(s) 1063, 1070, 1376, 2569, 2710, 3425
NmuCI GTSAC 1 cut(s) 49
NsiI ATGCAT 2 cut(s) 1478, 1719
NspI RCATGY 3 cut(s) 1476, 2284, 2751
PaeI GCATGC 1 cut(s) 1476
PagI TCATGA 1 cut(s) 801
PciI ACATGT 1 cut(s) 2747
PciSI GCTCTTC 2 cut(s) 1021, 2778
PcsI WCGNNNNNNNCGW 1 cut(s) 2488
PctI GAATGC 4 cut(s) 470, 811, 1340, 2743
PflFI GACNNNGTC 1 cut(s) 2484
PflMI CCANNNNNTGG 1 cut(s) 1882
PkrI GCNGC 5 cut(s) 606, 775, 1414, 1538, 1872
PleI GAGTC 4 cut(s) 848, 1465, 1985, 3369
PmaCI CACGTG 1 cut(s) 3083
PmlI CACGTG 1 cut(s) 3083
PpsI GAGTC 4 cut(s) 848, 1465, 1985, 3369
Ppu21I YACGTR 5 cut(s) 1319, 2759, 3049, 3083, 3199
PpuMI RGGWCCY 1 cut(s) 2532
PscI ACATGT 1 cut(s) 2747
PsiI TTATAA 2 cut(s) 666, 3120
Psp1406I AACGTT 1 cut(s) 456
Psp5II RGGWCCY 1 cut(s) 2532
Psp6I CCWGG 3 cut(s) 2802, 3093, 3132
PspCI CACGTG 1 cut(s) 3083
PspFI CCCAGC 2 cut(s) 991, 2594
PspGI CCWGG 3 cut(s) 2802, 3093, 3132
PspN4I GGNNCC 6 cut(s) 1063, 1070, 1376, 2569, 2710, 3425
PspOMI GGGCCC 1 cut(s) 1374
PspPI GGNCC 7 cut(s) 1374, 1375, 1660, 2532, 2708, 2875, 3071
PspPPI RGGWCCY 1 cut(s) 2532
PsuI RGATCY 3 cut(s) 1068, 1938, 1984
PsyI GACNNNGTC 1 cut(s) 2484
PvuII CAGCTG 1 cut(s) 194
RsaI GTAC 6 cut(s) 8, 528, 2279, 2904, 3311, 3467
RsaNI GTAC 6 cut(s) 7, 527, 2278, 2903, 3310, 3466
RseI CAYNNNNRTG 3 cut(s) 1452, 1815, 2654
SapI GCTCTTC 2 cut(s) 1021, 2778
SatI GCNGC 5 cut(s) 605, 774, 1413, 1537, 1871
Sau3AI GATC 8 cut(s) 57, 514, 590, 1068, 1938, 1984, 2846, 3007
Sau96I GGNCC 7 cut(s) 1374, 1375, 1660, 2532, 2708, 2875, 3071
ScaI AGTACT 1 cut(s) 8
SchI GAGTC 4 cut(s) 849, 1466, 1986, 3370
ScrFI CCNGG 4 cut(s) 1066, 2804, 3095, 3134
SduI GDGCHC 2 cut(s) 895, 1378
SinI GGWCC 4 cut(s) 2532, 2708, 2875, 3071
SmiMI CAYNNNNRTG 3 cut(s) 1452, 1815, 2654
SmlI CTYRAG 5 cut(s) 77, 2111, 2536, 2879, 3358
SmoI CTYRAG 5 cut(s) 77, 2111, 2536, 2879, 3358
SnaBI TACGTA 1 cut(s) 3199
SphI GCATGC 1 cut(s) 1476
SsiI CCGC 3 cut(s) 1768, 2484, 2854
SspI AATATT 1 cut(s) 2344
SspMI CTAG 7 cut(s) 210, 564, 1329, 1674, 1692, 2202, 2421
StyD4I CCNGG 4 cut(s) 1064, 2802, 3093, 3132
StyI CCWWGG 2 cut(s) 1200, 1957
TaaI ACNGT 8 cut(s) 25, 1366, 2169, 2277, 2291, 2669, 2907, 3262
TaiI ACGT 9 cut(s) 162, 459, 1321, 2224, 2761, 2957, 3051, 3085, 3201
TaqI TCGA 9 cut(s) 18, 256, 286, 1381, 1417, 2436, 2457, 2845, 3225
TatI WGTACW 4 cut(s) 6, 2277, 2902, 3309
TscAI CASTG 4 cut(s) 614, 2002, 2203, 3170
TseFI GTSAC 1 cut(s) 49
TseI GCWGC 5 cut(s) 604, 773, 1412, 1536, 1870
Tsp45I GTSAC 1 cut(s) 49
TspGWI ACGGA 1 cut(s) 861
TspRI CASTG 4 cut(s) 614, 2002, 2203, 3170
Tth111I GACNNNGTC 1 cut(s) 2484
Van91I CCANNNNNTGG 1 cut(s) 1882
Vha464I CTTAAG 1 cut(s) 77
VpaK11BI GGWCC 4 cut(s) 2532, 2708, 2875, 3071
XapI RAATTY 9 cut(s) 489, 637, 966, 1122, 1571, 1860, 2690, 3016, 3474
XceI RCATGY 3 cut(s) 1476, 2284, 2751
XcmI CCANNNNNNNNNTGG 1 cut(s) 436
XmiI GTMKAC 3 cut(s) 843, 1345, 3195
XspI CTAG 7 cut(s) 210, 564, 1329, 1674, 1692, 2202, 2421
ZrmI AGTACT 1 cut(s) 8
Zsp2I ATGCAT 2 cut(s) 1478, 1719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.