RchiOBHm_Chr3g0473531

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000085
Physical Location & Seq
Forward (+)
19348693 .. 19354143
5451 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43919

Sequence Viewer

Length: 3480 bp
ATGTCAGAGGATAATCAAGAATCGGTTGATGTTGATGCCTCTGCATCTCTTCCTTCATCATCAGCTCCTCGGTGGAAGCATGATGTGTTTTTGAGTTTCAGGGGTGCAGACACTCGCAAGGGAATTGCATTTGAATTATACGATCGACTGCAAAACAGGAGAGGAATCAAAACATTCATGGATGACCAAGACCTTCAAGTAGGGGATGTTATTTCTCCCACTCTCCTAACGGCAATTAAAGAATCAAGGTTTGCAATTATTGTTCTCTCTAAAAACTATGCCTCTTCCACTTGGTGTTTGGAGGAACTTAGAAGCATCTGTGAGTGCATGGAAGAAGACAACAATAGAATTCTGCCACTTTTTTATTATGTGGATCCTACTGATGTACGATATCAGAAGAGGAGTTTCGGAGATGCTTTCACTAAGCATGAAAACTCTGGGCGACACAGATCAGAGAAGGTGCAGCAGTGGAGAGATTCTTTAAAAAAAGTGGCAAATTTCTCTGGATGGCATATACTGAATCATAAATCTGAAAGAGAACTTTTGGATGCCATTGAGGAATCTGTGTGCAATAAATTACGACCTACTGAACTTGAGTTTAAATTGTCCATCGGAGGTTTTGAAGCATTTGCAGCAACAAAACAAGCCATTGATAAGGTCATGAATGCTCTAAAAGATGATGAGGCCACTACCATTGGAGTCTACGGAATGGGGGGCGTTGGAAAGACAGCAGTGGTGAAATATGTTGGAGCACAAGCCCAAAAAAGTAGGCTTTTTAATCAAGTGATTATGGCTGTCGTATCCCAAAACCCCGACTTGATAAAAATTCAAGAGACATTTGCAGAACTGCTGGGCTTTAAATTGGAGGAGAAGACAGAAATTGGAAGATCCATTAAATTGAAGGAGAAGATAATGAGAGGAACCGGGATCCTCATAATCTTGGATGACATTTGGAAGAGAATAGACTTTTCAAGCATAGGAATTCCAAGCCACAACGAACTTCAAAGATGCAATTCCAAAGTCCTACTGACCACCAGAAGATTGACTGTTTGTCAGATCATGGATAGCCATGCAAAAATTCATCTCAATATCTTATCAAAAGAAGATTCTTGGAGCTTATTTTTGAAGGAAGCAAGGAAATCTTTTGACAAATCTTCCAATTTCTATGTTGTAGCGAGTGAGGTGGCAAGAGAATGCGCCGGTCTACCAGTTGCCTTGATAGCAGTTGCGAGGGCCCTTCGAGATGAAGGTTTGGACGGATGGAAAGAAGCTGCTCGACGACTGAAAGCATCTCAACCTCCCTTCCCTGAAGATGAGGGAGATGTTTTCAAATGCATAAAGTTGAGTTATGATTACTTGAAATCTGATGATTCCAAATCATGCTTCTTGCTTTGCTGCCTGTTCCCAGAAGATTATGATATCCCAATTGAATACTTGTTCAAGTATGGAATTGGGAAAGGAATGTTTCAAGATTCAAACTTGCTACAGGCCCGAGCCACAACATATTTAGTGGTGAAGGCCCTTAAAGATTCTAGTTTGCTTTTGGACGCTAGAAATGACAGATGTGTAAAGATGCATGATGTCATTCGGGATATGGCAATCTCAATTTTGTTATCTGAAGGACAGCCGGTCTTGGTGAAAGCTGGCTGTGAATTGAAGAATTGGCCACCGATTGATGCACATAAAGCCTACTCTGCAATCTCACTAATGGAGAACAAAATTTGCAAGCTACCCCAAGAGTTGGTATGTCCAAATCTCCAGATTTTATTACTGCAACACAATGCTTCATTAAATGATATCCCAAACTCTTTCTTCCAATGTCAGAAAGAATTAGGAGTCTTAGATCTTAGCGGCACTGGTATTTCATTACTACCGCACTCAATCAGTTTCCTAACCAACCTTCAAGCTTTGTATTTAGATTATTGCAAGAACATAATTGACATTTCTGTAATCGGAAAACTTAACAAGCTTGAGATTCTTAGTATGAGAGAAAATACTCAGAGGGAATTGCCAAGGGAAATAGGACGATTGACCAATCTAAGGATGTTGGATATCAGTAGTGATTCTATACCCCAATCCCCATATGGATATAATCGCACAATTCCATCTAAAGTGATATCAAAGTTGCATAAATTAGAAGAACTGTACATGCAATGTGTATTTTGGGACTGGGGGAGTAAAATTGTTGGAGATGGAGAAGAAACTAATATTGGCTTTGATGAATTGGCTGGTTTATCATATTTAAGCATTTTGAAAGTTTGCATATCTGATGCAAATTGCATCCCTAAAACTGTTGATGTCGAACCGAATTGGGTTTACTTTGATATTAGAATTTCCAGCAGACATATGAGGGACAGTGTGAACTTACAATCTGGTCATAATTGTAGAACCTTAAGTCTTGATAGCACTGACACAACCATAGGAACCTTTCCGGACTGGTTTGTCAACGCTGCGGTAAAGAATACTGAGAGGCTATGTTATGAAGAATGCCGAGGGTTAAGTAACATTCTTGTTGAATATGACCGTGGTAGGTTACATGGACTGAAAGTTCTCTCTGTAAGAGGTTGCTGTGAGAACTTGAAAGAGTTGATGAACGCAAAAACATGCGTTTCTGATAAGCCTGTGTTTGAGAACTTGGAAGAGTTGTATTTGAAAGACATGGATGACCTGAAACAGTTATGTGTTGGTGAGTTACCACCTGGATCTCTCTGCAGTCTCAAATTATTGAAGGTCTGTAAGTGTCCTAACTTGGAGAATGTGCTGTTGCCATCAGAATTGTTGCAGAAACTACCAAATCTGGAAGAACTATTCTGTGAGGCAAGTGGAGTGGAATATGTGTTTGGATGTGAAGGATTTGAGCCGGATCAAACAAATCTGAGAAAGATGGAGTTGATGGATCTAGACGTAGTAAGAAGCATATGTAATGGTCCTGCTCCAAGTGGAATGTTCCAAGCTCTGATGGAATTGGTCATTATGTATTGCAATTTCCAGGGAAGCCTGTTCACATTTGATGTCTCTCAGTGTCTTTTTCAATTGGAAACCCTTACTGTATTCGAATGCCCTGTCTTGGAAAGAGTAATCGAAGCAAGGAGGGAAACATTGAAGAACAAGAAGACCATTCTTCCAAAACTGAAGAACTTATGTTTGATAGATCTTCCAATGTTGTACAAGGGAGGTGCTACTATTGATTTTGACTGTCCTTCATTGGAAAACTTTTATATGGAGGATTGCCCTCACTTGTCATTTCCGTCCTCTGCTTCTGACTACTTCCACAGCAGGAACCAAGTCAATGGTGATAAGCCTAAAAACTGTATCCCACTCCTCGATATAAATCTTCGAAAAGTGGAAAGAAGGCAACGAAAGTATGCCGCGGAACTCCTCGACATGGACAGACTGCTAGAGCACTTGCAAAACAGGCAAACGGAACTGAGGATAATGCTAGAAAATCAAGACAGCCCGGGGGTGGAAGAGGTAGAGTGA

Protein Analysis

1159

Amino Acids

131.48

Weight (kDa)

5.57

Isoelectric Point (pI)

45.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 26 - 197 3.1e-50 TIR domain
TIR_2 PF13676 29 - 139 5.1e-17 TIR domain
NB-ARC PF00931 214 - 379 7.3e-29 NB-ARC domain
LRR_8 PF13855 584 - 642 2.3e-07 Leucine rich repeat
LRR_14 PF23598 609 - 800 1.1e-13 Leucine-rich repeat region
LRR_RPS2 PF23247 819 - 937 7.2e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 879 - 1020 9.5e-18 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 960 - 1084 7.1e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2441
AccB7I CCANNNNNTGG 1 cut(s) 1741
AccI GTMKAC 2 cut(s) 702, 1204
AccII CGCG 1 cut(s) 3371
AccIII TCCGGA 1 cut(s) 2431
AciI CCGC 5 cut(s) 1851, 1874, 2453, 3369, 3371
AclWI GGATC 8 cut(s) 368, 381, 882, 922, 935, 2710, 2871, 2904
AcoI YGGCCR 1 cut(s) 1664
AcsI RAATTY 7 cut(s) 348, 496, 825, 981, 1077, 1719, 2331
AcuI CTGAAG 3 cut(s) 1329, 1638, 3152
AdeI CACNNNGTG 1 cut(s) 294
AfaI GTAC 3 cut(s) 387, 2147, 3167
AfiI CCNNNNNNNGG 5 cut(s) 1741, 2040, 3067, 3385, 3463
AflII CTTAAG 1 cut(s) 2392
AhdI GACNNNNNGTC 2 cut(s) 1050, 1628
AjnI CCWGG 2 cut(s) 2698, 2988
AjuI GAANNNNNNNTTGG 6 cut(s) 1367, 1399, 2193, 2225, 2823, 2855
AloI GAACNNNNNNTCC 2 cut(s) 2532, 2564
AluBI AGCT 8 cut(s) 65, 1116, 1271, 1643, 1729, 1907, 1969, 2954
AluI AGCT 8 cut(s) 65, 1116, 1271, 1643, 1729, 1907, 1969, 2954
Alw21I GWGCWC 2 cut(s) 754, 3405
Alw26I GTCTC 3 cut(s) 827, 2720, 3019
AlwI GGATC 8 cut(s) 368, 381, 882, 922, 935, 2710, 2871, 2904
Ama87I CYCGRG 2 cut(s) 1491, 3457
Aor13HI TCCGGA 1 cut(s) 2431
AoxI GGCC 5 cut(s) 684, 1233, 1488, 1518, 1664
ApaI GGGCCC 1 cut(s) 1237
ApeKI GCWGC 5 cut(s) 463, 632, 1271, 1395, 2450
ApoI RAATTY 7 cut(s) 348, 496, 825, 981, 1077, 1719, 2331
ArsI GACNNNNNNTTYG 2 cut(s) 3048, 3080
AspLEI GCGC 1 cut(s) 1199
AspS9I GGNCC 5 cut(s) 1233, 1234, 1489, 1519, 2927
AsuC2I CCSGG 3 cut(s) 925, 3458, 3459
AsuHPI GGTGA 5 cut(s) 748, 1525, 1648, 2699, 3305
AsuII TTCGAA 2 cut(s) 3054, 3337
AvaI CYCGRG 2 cut(s) 1491, 3457
AvaII GGWCC 1 cut(s) 2927
BaeGI GKGCMC 1 cut(s) 1237
BalI TGGCCA 1 cut(s) 1666
BamHI GGATCC 2 cut(s) 373, 927
BanII GRGCYC 1 cut(s) 1237
BbsI GAAGAC 3 cut(s) 342, 878, 3119
Bbv12I GWGCWC 2 cut(s) 754, 3405
BbvI GCAGC 5 cut(s) 475, 644, 1258, 1382, 2437
BccI CCATC 9 cut(s) 501, 617, 1254, 2113, 2186, 2776, 2878, 2887, 2953
BceAI ACGGC 1 cut(s) 246
BciT130I CCWGG 2 cut(s) 2700, 2990
BciVI GTATCC 2 cut(s) 811, 3323
BcnI CCSGG 3 cut(s) 925, 3458, 3459
BcoDI GTCTC 3 cut(s) 827, 2720, 3019
BfaI CTAG 5 cut(s) 1533, 1551, 2900, 3398, 3440
BfmI CTRYAG 2 cut(s) 1484, 2710
BfrI CTTAAG 1 cut(s) 2392
BfuI GTATCC 2 cut(s) 811, 3323
BglII AGATCT 2 cut(s) 1843, 3151
BisI GCNGC 7 cut(s) 464, 633, 1272, 1396, 1852, 2451, 3369
BlsI GCNGC 7 cut(s) 465, 634, 1273, 1397, 1853, 2452, 3370
Bme1390I CCNGG 5 cut(s) 925, 2700, 2990, 3458, 3459
Bme18I GGWCC 1 cut(s) 2927
BmeRI GACNNNNNGTC 2 cut(s) 1050, 1628
BmeT110I CYCGRG 2 cut(s) 1491, 3457
BmgT120I GGNCC 5 cut(s) 1233, 1234, 1489, 1519, 2927
BmiI GGNNCC 6 cut(s) 375, 922, 929, 1235, 2425, 3281
BmrFI CCNGG 5 cut(s) 925, 2700, 2990, 3458, 3459
BmrI ACTGGG 1 cut(s) 2179
BmuI ACTGGG 1 cut(s) 2179
BpiI GAAGAC 3 cut(s) 342, 878, 3119
BpmI CTGGAG 1 cut(s) 1742
Bpu14I TTCGAA 2 cut(s) 3054, 3337
BpuEI CTTGAG 2 cut(s) 614, 1991
BpuMI CCSGG 3 cut(s) 925, 3458, 3459
BsaBI GATNNNNATC 1 cut(s) 3330
BsaJI CCNNGG 8 cut(s) 68, 2012, 2491, 2524, 2989, 3369, 3457, 3458
BsaWI WCCGGW 1 cut(s) 2431
Bsc4I CCNNNNNNNGG 5 cut(s) 1741, 2040, 3067, 3385, 3463
Bse118I RCCGGY 2 cut(s) 1199, 1627
Bse1I ACTGG 4 cut(s) 1208, 1861, 2174, 2441
Bse3DI GCAATG 1 cut(s) 2159
Bse8I GATNNNNATC 1 cut(s) 3330
BseAI TCCGGA 1 cut(s) 2431
BseBI CCWGG 2 cut(s) 2700, 2990
BseDI CCNNGG 8 cut(s) 68, 2012, 2491, 2524, 2989, 3369, 3457, 3458
BseJI GATNNNNATC 1 cut(s) 3330
BseLI CCNNNNNNNGG 5 cut(s) 1741, 2040, 3067, 3385, 3463
BseMI GCAATG 1 cut(s) 2159
BseMII CTCAG 5 cut(s) 2012, 2457, 2867, 3032, 3419
BseNI ACTGG 4 cut(s) 1208, 1861, 2174, 2441
BseRI GAGGAG 5 cut(s) 57, 415, 881, 3311, 3368
BseSI GKGCMC 1 cut(s) 1237
BseXI GCAGC 5 cut(s) 475, 644, 1258, 1382, 2437
BseYI CCCAGC 1 cut(s) 850
BsgI GTGCAG 2 cut(s) 126, 482
Bsh1236I CGCG 1 cut(s) 3371
Bsh1285I CGRYCG 1 cut(s) 145
BshFI GGCC 5 cut(s) 686, 1235, 1490, 1520, 1666
BsiEI CGRYCG 1 cut(s) 145
BsiHKAI GWGCWC 2 cut(s) 754, 3405
BsiHKCI CYCGRG 2 cut(s) 1491, 3457
BsiSI CCGG 6 cut(s) 924, 1200, 1628, 2432, 2861, 3458
BslFI GGGAC 2 cut(s) 2180, 2366
BslI CCNNNNNNNGG 5 cut(s) 1741, 2040, 3067, 3385, 3463
BsmAI GTCTC 3 cut(s) 827, 2720, 3019
BsmFI GGGAC 2 cut(s) 2180, 2366
BsmI GAATGC 4 cut(s) 670, 1199, 2492, 3062
BsnI GGCC 5 cut(s) 686, 1235, 1490, 1520, 1666
BsoBI CYCGRG 2 cut(s) 1491, 3457
Bsp119I TTCGAA 2 cut(s) 3054, 3337
Bsp120I GGGCCC 1 cut(s) 1233
Bsp1286I GDGCHC 3 cut(s) 754, 1237, 3405
Bsp13I TCCGGA 1 cut(s) 2431
Bsp1407I TGTACA 2 cut(s) 2145, 3165
BspACI CCGC 5 cut(s) 1851, 1874, 2453, 3369, 3371
BspANI GGCC 5 cut(s) 686, 1235, 1490, 1520, 1666
BspCNI CTCAG 5 cut(s) 2011, 2458, 2868, 3031, 3420
BspEI TCCGGA 1 cut(s) 2431
BspFNI CGCG 1 cut(s) 3371
BspHI TCATGA 1 cut(s) 660
BspLI GGNNCC 6 cut(s) 375, 922, 929, 1235, 2425, 3281
BspMAI CTGCAG 1 cut(s) 2714
BspPI GGATC 8 cut(s) 368, 381, 882, 922, 935, 2710, 2871, 2904
BspT104I TTCGAA 2 cut(s) 3054, 3337
BspTI CTTAAG 1 cut(s) 2392
BsrDI GCAATG 1 cut(s) 2159
BsrFI RCCGGY 2 cut(s) 1199, 1627
BsrGI TGTACA 2 cut(s) 2145, 3165
BsrI ACTGG 4 cut(s) 1208, 1861, 2174, 2441
BssAI RCCGGY 2 cut(s) 1199, 1627
BssECI CCNNGG 8 cut(s) 68, 2012, 2491, 2524, 2989, 3369, 3457, 3458
BssT1I CCWWGG 1 cut(s) 2012
Bst2UI CCWGG 2 cut(s) 2700, 2990
Bst4CI ACNGT 9 cut(s) 1048, 2145, 2293, 2357, 2525, 2676, 3049, 3197, 3311
Bst6I CTCTTC 6 cut(s) 54, 289, 392, 950, 2634, 3462
BstAFI CTTAAG 1 cut(s) 2392
BstAUI TGTACA 2 cut(s) 2145, 3165
BstBI TTCGAA 2 cut(s) 3054, 3337
BstC8I GCNNGC 2 cut(s) 1645, 1727
BstDSI CCRYGG 2 cut(s) 2524, 3369
BstFNI CGCG 1 cut(s) 3371
BstHHI GCGC 1 cut(s) 1199
BstMAI GTCTC 3 cut(s) 827, 2720, 3019
BstMCI CGRYCG 1 cut(s) 145
BstMWI GCNNNNNNNGC 5 cut(s) 632, 1220, 1685, 1694, 3415
BstNI CCWGG 2 cut(s) 2700, 2990
BstNSI RCATGY 2 cut(s) 2152, 2607
BstSCI CCNGG 5 cut(s) 923, 2698, 2988, 3456, 3457
BstSFI CTRYAG 2 cut(s) 1484, 2710
BstSLI GKGCMC 1 cut(s) 1237
BstUI CGCG 1 cut(s) 3371
BstV1I GCAGC 5 cut(s) 475, 644, 1258, 1382, 2437
BstV2I GAAGAC 3 cut(s) 342, 878, 3119
BstX2I RGATCY 7 cut(s) 373, 887, 927, 1843, 2702, 2896, 3151
BstXI CCANNNNNNTGG 1 cut(s) 3290
BstYI RGATCY 7 cut(s) 373, 887, 927, 1843, 2702, 2896, 3151
BsuI GTATCC 2 cut(s) 811, 3323
BsuRI GGCC 5 cut(s) 686, 1235, 1490, 1520, 1666
BtgI CCRYGG 2 cut(s) 2524, 3369
BtsI GCAGTG 2 cut(s) 473, 738
BtsIMutI CAGTG 6 cut(s) 473, 738, 1854, 2362, 2406, 3026
Cac8I GCNNGC 2 cut(s) 1645, 1727
CciI TCATGA 1 cut(s) 660
CfoI GCGC 1 cut(s) 1199
Cfr10I RCCGGY 2 cut(s) 1199, 1627
Cfr13I GGNCC 5 cut(s) 1233, 1234, 1489, 1519, 2927
Cfr42I CCGCGG 1 cut(s) 3372
Cfr9I CCCGGG 1 cut(s) 3457
CseI GACGC 1 cut(s) 1556
Csp6I GTAC 3 cut(s) 386, 2146, 3166
CspCI CAANNNNNGTGG 4 cut(s) 676, 711, 2808, 2843
CviQI GTAC 3 cut(s) 386, 2146, 3166
DraI TTTAAA 3 cut(s) 483, 601, 859
DraIII CACNNNGTG 1 cut(s) 294
DrdI GACNNNNNNGTC 1 cut(s) 2441
DriI GACNNNNNGTC 2 cut(s) 1050, 1628
DseDI GACNNNNNNGTC 1 cut(s) 2441
EaeI YGGCCR 1 cut(s) 1664
Eam1104I CTCTTC 6 cut(s) 54, 289, 392, 950, 2634, 3462
Eam1105I GACNNNNNGTC 2 cut(s) 1050, 1628
EarI CTCTTC 6 cut(s) 54, 289, 392, 950, 2634, 3462
Eco130I CCWWGG 1 cut(s) 2012
Eco24I GRGCYC 1 cut(s) 1237
Eco32I GATATC 5 cut(s) 392, 1420, 1798, 2053, 2118
Eco47I GGWCC 1 cut(s) 2927
Eco57I CTGAAG 3 cut(s) 1329, 1638, 3152
Eco88I CYCGRG 2 cut(s) 1491, 3457
EcoO109I RGGNCCY 3 cut(s) 1233, 1234, 1519
EcoRI GAATTC 2 cut(s) 348, 981
EcoRII CCWGG 2 cut(s) 2698, 2988
EcoRV GATATC 5 cut(s) 392, 1420, 1798, 2053, 2118
EcoT14I CCWWGG 1 cut(s) 2012
EcoT22I ATGCAT 2 cut(s) 1337, 1578
EcoT38I GRGCYC 1 cut(s) 1237
ErhI CCWWGG 1 cut(s) 2012
FalI AAGNNNNNCTT 2 cut(s) 1126, 1158
FaqI GGGAC 2 cut(s) 2180, 2366
FauNDI CATATG 3 cut(s) 2083, 2346, 2918
FblI GTMKAC 2 cut(s) 702, 1204
Fnu4HI GCNGC 7 cut(s) 464, 633, 1272, 1396, 1852, 2451, 3369
FriOI GRGCYC 1 cut(s) 1237
Fsp4HI GCNGC 7 cut(s) 464, 633, 1272, 1396, 1852, 2451, 3369
FspBI CTAG 5 cut(s) 1533, 1551, 2900, 3398, 3440
GlaI GCGC 1 cut(s) 1198
GluI GCNGC 7 cut(s) 464, 633, 1272, 1396, 1852, 2451, 3369
GsaI CCCAGC 1 cut(s) 854
GsuI CTGGAG 1 cut(s) 1742
HaeIII GGCC 5 cut(s) 686, 1235, 1490, 1520, 1666
HapII CCGG 6 cut(s) 924, 1200, 1628, 2432, 2861, 3458
HgaI GACGC 1 cut(s) 1556
HhaI GCGC 1 cut(s) 1199
Hin6I GCGC 1 cut(s) 1197
HinP1I GCGC 1 cut(s) 1197
HincII GTYRAC 1 cut(s) 2446
HindII GTYRAC 1 cut(s) 2446
HindIII AAGCTT 2 cut(s) 1905, 1967
HpaII CCGG 6 cut(s) 924, 1200, 1628, 2432, 2861, 3458
HphI GGTGA 5 cut(s) 748, 1525, 1648, 2699, 3305
Hpy166II GTNNAC 6 cut(s) 703, 1205, 2317, 2362, 2446, 3003
Hpy8I GTNNAC 6 cut(s) 703, 1205, 2317, 2362, 2446, 3003
Hpy99I CGWCG 1 cut(s) 1281
HpyCH4III ACNGT 9 cut(s) 1048, 2145, 2293, 2357, 2525, 2676, 3049, 3197, 3311
HpyCH4IV ACGT 1 cut(s) 2904
HpyF10VI GCNNNNNNNGC 5 cut(s) 632, 1220, 1685, 1694, 3415
HpySE526I ACGT 1 cut(s) 2904
HspAI GCGC 1 cut(s) 1197
Kpn2I TCCGGA 1 cut(s) 2431
KspI CCGCGG 1 cut(s) 3372
LmnI GCTCC 4 cut(s) 70, 749, 1113, 2938
Lsp1109I GCAGC 5 cut(s) 475, 644, 1258, 1382, 2437
MaeI CTAG 5 cut(s) 1533, 1551, 2900, 3398, 3440
MaeII ACGT 1 cut(s) 2904
MaeIII GTNAC 3 cut(s) 2501, 2532, 2691
MfeI CAATTG 2 cut(s) 1425, 3032
MflI RGATCY 7 cut(s) 373, 887, 927, 1843, 2702, 2896, 3151
MhlI GDGCHC 3 cut(s) 754, 1237, 3405
MlsI TGGCCA 1 cut(s) 1666
MluNI TGGCCA 1 cut(s) 1666
MlyI GAGTC 2 cut(s) 708, 1845
MmeI TCCRAC 4 cut(s) 700, 727, 2028, 2167
Mox20I TGGCCA 1 cut(s) 1666
Mph1103I ATGCAT 2 cut(s) 1337, 1578
MroI TCCGGA 1 cut(s) 2431
MscI TGGCCA 1 cut(s) 1666
Msp20I TGGCCA 1 cut(s) 1666
MspA1I CMGCKG 1 cut(s) 3371
MspCI CTTAAG 1 cut(s) 2392
MspI CCGG 6 cut(s) 924, 1200, 1628, 2432, 2861, 3458
MspR9I CCNGG 5 cut(s) 925, 2700, 2990, 3458, 3459
MunI CAATTG 2 cut(s) 1425, 3032
Mva1269I GAATGC 4 cut(s) 670, 1199, 2492, 3062
MvaI CCWGG 2 cut(s) 2700, 2990
MvnI CGCG 1 cut(s) 3371
MwoI GCNNNNNNNGC 5 cut(s) 632, 1220, 1685, 1694, 3415
NciI CCSGG 3 cut(s) 925, 3458, 3459
NdeI CATATG 3 cut(s) 2083, 2346, 2918
NlaIV GGNNCC 6 cut(s) 375, 922, 929, 1235, 2425, 3281
NmeAIII GCCGAG 1 cut(s) 2516
NsiI ATGCAT 2 cut(s) 1337, 1578
NspI RCATGY 2 cut(s) 2152, 2607
NspV TTCGAA 2 cut(s) 3054, 3337
PagI TCATGA 1 cut(s) 660
PctI GAATGC 4 cut(s) 670, 1199, 2492, 3062
PflMI CCANNNNNTGG 1 cut(s) 1741
PkrI GCNGC 7 cut(s) 465, 634, 1273, 1397, 1853, 2452, 3370
Ple19I CGATCG 1 cut(s) 145
PleI GAGTC 2 cut(s) 707, 1844
PpsI GAGTC 2 cut(s) 707, 1844
Psp6I CCWGG 2 cut(s) 2698, 2988
PspFI CCCAGC 1 cut(s) 850
PspGI CCWGG 2 cut(s) 2698, 2988
PspN4I GGNNCC 6 cut(s) 375, 922, 929, 1235, 2425, 3281
PspOMI GGGCCC 1 cut(s) 1233
PspPI GGNCC 5 cut(s) 1233, 1234, 1489, 1519, 2927
PstI CTGCAG 1 cut(s) 2714
PsuI RGATCY 7 cut(s) 373, 887, 927, 1843, 2702, 2896, 3151
PvuI CGATCG 1 cut(s) 145
RsaI GTAC 3 cut(s) 387, 2147, 3167
RsaNI GTAC 3 cut(s) 386, 2146, 3166
SacII CCGCGG 1 cut(s) 3372
SatI GCNGC 7 cut(s) 464, 633, 1272, 1396, 1852, 2451, 3369
Sau96I GGNCC 5 cut(s) 1233, 1234, 1489, 1519, 2927
SchI GAGTC 2 cut(s) 708, 1845
ScrFI CCNGG 5 cut(s) 925, 2700, 2990, 3458, 3459
SduI GDGCHC 3 cut(s) 754, 1237, 3405
SfcI CTRYAG 2 cut(s) 1484, 2710
Sfr303I CCGCGG 1 cut(s) 3372
SfuI TTCGAA 2 cut(s) 3054, 3337
SgrBI CCGCGG 1 cut(s) 3372
SinI GGWCC 1 cut(s) 2927
SmaI CCCGGG 1 cut(s) 3459
SmlI CTYRAG 3 cut(s) 593, 1970, 2392
SmoI CTYRAG 3 cut(s) 593, 1970, 2392
SsiI CCGC 5 cut(s) 1851, 1874, 2453, 3369, 3371
SspI AATATT 1 cut(s) 2209
SspMI CTAG 5 cut(s) 1533, 1551, 2900, 3398, 3440
StyD4I CCNGG 5 cut(s) 923, 2698, 2988, 3456, 3457
StyI CCWWGG 1 cut(s) 2012
TaaI ACNGT 9 cut(s) 1048, 2145, 2293, 2357, 2525, 2676, 3049, 3197, 3311
TaiI ACGT 1 cut(s) 2907
TaqI TCGA 9 cut(s) 145, 1240, 1276, 2301, 3054, 3081, 3324, 3337, 3381
TatI WGTACW 2 cut(s) 2145, 3165
TauI GCSGC 2 cut(s) 1854, 3371
TscAI CASTG 6 cut(s) 473, 738, 1861, 2362, 2413, 3026
TseI GCWGC 5 cut(s) 463, 632, 1271, 1395, 2450
TspGWI ACGGA 4 cut(s) 720, 1272, 3237, 3437
TspMI CCCGGG 1 cut(s) 3457
TspRI CASTG 6 cut(s) 473, 738, 1861, 2362, 2413, 3026
Van91I CCANNNNNTGG 1 cut(s) 1741
Vha464I CTTAAG 1 cut(s) 2392
VpaK11BI GGWCC 1 cut(s) 2927
XapI RAATTY 7 cut(s) 348, 496, 825, 981, 1077, 1719, 2331
XbaI TCTAGA 1 cut(s) 2899
XceI RCATGY 2 cut(s) 2152, 2607
XcmI CCANNNNNNNNNTGG 2 cut(s) 295, 2081
XmaI CCCGGG 1 cut(s) 3457
XmiI GTMKAC 2 cut(s) 702, 1204
XspI CTAG 5 cut(s) 1533, 1551, 2900, 3398, 3440
Zsp2I ATGCAT 2 cut(s) 1337, 1578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.