RchiOBHm_Chr5g0079881

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000085
Physical Location & Seq
Reverse (-)
85691006 .. 85699755
8750 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35423

Sequence Viewer

Length: 3942 bp
ATGGCCGCTTCTTCATCTTCATCTTCTTCTGGTGGTCATCGGAAGTATGATGTCTTCCTCAGTTTTAGAGGACCAGACACCCGCCAGGTCTTCGTTGGCCATCTGTACAGAGCTCTGCAAAAGAAAGCAATCGACACCTTCATCGATATAATAGATCCCGGAAAAGGCTCCATTATTTACAAGTTACTGAACTTAATTGACGAATCGAGGATTTCGGTTGTAGTTTTATCTGAAAACTATGCCTTTTCCACATCGTGCTTAAAAGAGCTCGTCAAAATCATGGAATGCATGGATAAGAAGAAGCAGATTGTGGTGCCCATTTTCTATAAAGTAGATCCCTTCGATATTCGATATTTGAAGAACAGCTTTGCGAAAGCTTTTGCCAGACATGAACATATGGGTCGTTCTAGCAATAAAGAGTTGGAGACCTGGAAAACTGCACTAAAAAGAATCACGGATCTATCTGGCTGGGACTCGCGAAAGTATGAGGATGACGCAGTTCTTGTTGAAGTCATTGTGAAATTTGTGTGTAGTAAACTACAACCTGTTGTGGAAATTGAAGCAACAAGACAAGCCACTATTGAAATTGAGCCTACAATGTCGGAGTCAAATGAAGACTTTGAAGTAGTTAAAGTAATAAGACGAGCTCCAACTGAAATTGAGCCTACAATGCCAAAGTTGACTGAAGATTTTGAAGGAGTTGAATCAACAACACAAGCTACCAATGGAAACTCTGAACCAGCTCCTCAGTGGAACCATGATGTGTTTTTGAGCTTTAGGGGTGATGACACTCGTAAGGGTTTTCTATCCCATTTATACCATGAATTGCAAAACACGAAAGTTATTAGAACATTTAAGGATGATGAACAACTTAAGAAAGGGAAGGATATTTCTCAAAGTCTGCTGAGGGCAATTGAAGAATCAAGGTTTGCGATAGTTGTTCTATCAGAAAACTATGCTTTTTCTGCATGGTGTTTAGATGAACTTACAAAGATTTTTCAATGCATGGAAGGCAAGGATACAATTCTGCCAATTTTTTATCATGCGGATCCCTCTGATGTACGATATCAAAAGGGTCAATTTGAAGTGGCCTTTACAAAGCATGAAAGCAAGGCAAGGTATGACATAGAGAAGGTGAAGCAGTGGAGAGCTGATTTAACAAAAGTGGGAAATCTCTCTGGGTGGGATTCAAAGAACCACAAATCCGAAAGGGAACTCGTTGAAGACATTGTGAAATTTGTGCTGAAAGAAGTACTTCAAGCACCAAGTGGAGATTTTGAAGAATTTGAAGCAACAAGAGAAGCCATGGATGAGGTTAAGAAGATGCTGAAAGATGATAAGATCACCGCCATTGGTGTCTATGGAAGAGGAGGCGTTGGCAAGACAGCCATGGTGAAACATGTCGGTGCACAAGCCAAAAGAATTGGGCTTTTTGATCATGTTGTTATGGCTGTTATATCCCAAAATCCTGATTTGCAAAAAATTCAAGGCGCATTGGTAAATCAGCTTGGATTGGAATTGGTGGAGGATGAAGAAATAGAAATAGCCAATTTGTCCAGCATTGGAATTCCTAGCTATGACGAGCTTCAAAGATGCAATTCCAAGGTCCTACTCACCACAAGGATCATGGATATTTGTCATTCTATGGAGTGCCAAGCACATGTTCTTCTCAATATCCTATCAGAGCAAGATTCTTTGAAGTTATTCATGAAGAAGTCGAGAAAGCCTTTTCATGGATCTACCGATTCCTATGATGAAGAGAGGAACGTAGTTAGAAAATGCTCTGGTCTCCCAAGTGTATTGGAAGAAGCTGCAAGGGAATTTGGACATAAATACGTGGAAGAATGGAACAAAGCAGTTTCAAAGGCATTTGGAGATGATGATGATGAATTGCAAAAAGCAGCTCAAGAACTAAATGCATCTCAACTTGTCCCCACTAAAGATGAGAGAGATACATTTAAAAGGATAGAGTTAAGCTATCATGCCTTGAGGTTTGAAGATGCCAAGTCATTTTTCTTGCTTTGCTGCATGTTTCCAGAAGATTATGATATCCCAATTGAAGATTTGTTTAAGTATGGGATTGGAAAAGGTTTGCTTCAGTATTCAGACACATTGCAAGAAGCCAGAGACAAAGCACATTCCTTGGTTGCAGACCTTAAAGCTTTGGGGTTGCTTCTGGATGGCAAGAAGGGATGCATTAGGATGCATTTTGACATCCAAAATGTGGCCATGTCTATTGCGTTGTCTGAAGGTCATGCTTTTTTTGTAAAAGCTGGTTGTGAATTGAAGGATTGGCCAATGGATGCTCATGAAGTCTACTCAGCAATCTCACTAATGAGGAACAGACTTAACAAGCTTCCTGAAACTTTGGTATGTTCTGAACTCCAAATTTTACTGCTGCAAAGTAATGCTGGTATATGTGACATTCCAAAAACTTTTTTTCAAAGTCCAAATGCACTAAGGGTCTTTGATCTTAGCTATACTGGTATTTCAGTACTGCCCTCTTCATTCAATGTCCTCACCAACCTCCAAGCCTTGTTTTTGGATTGTTGCAATAAAATAACTGACATATCCGTACTTAGGAAGCTGAAGAAAGTTGAGATTCTCAGTTTGAGAGAATTCCCTCTTAAGGAGTTGCCGAAAGAGATAGGAAGTTTGAACAGTCTAAGGATGCTGGATGTCACTGGTGGATGTGTTCACAAAATTCCGTCTAAAGTGATATCGAGGCTGTATAGACTGGAAGAACTGTACATGAAATGTGAATTTGGGAACTGGGGAAGTACAGTCGAGGAAGCAGTAGGACAAACAATTGCCGGCTTTGATGAGATAATTGGCTTGTCCCGGTTAAACATTTTGAAGGTTTACATATCTGATGCAGCTTGCTTACCCAAAGATATTGGGTTCTTTGGGCCGAATTGGGTAAAATTTGACATATGCATCAGCAGAGATCTCTCTCCGGAGATGATGCTTTCTCCGAATGATCAACCTTCAAGAACCTTGACTCTTGACAGAACAATTAATGGCTTATCGGATTGGTTTATCAACGTGGTGACGGAGAAAGCAGAAAAACTAGAGTACAGAGAGTGCAAGGGATTAACCAACATTCTTAAGGAATATGAGCATGGGAGATTACATGGACTCAAGCATCTTTCTATAATTGGGTGCCCTGAGAACTGGGAAGAGTTGATAAATGCAGCAACATGGGATCCAAGTAAACCTGTGTTTGAGAGCTTGGAAAAGTTGCATCCCCTTCAACTGCAACTCCTCAAGGAGTTATGTGTTGGTGAATTACCACTTGGGTCTCTATGCAATCTCAAGTTATTGAAAGTGCAAACTTGTTGTAACTTGATGAATGCACTTTTACCACCAAAATTGTTGCAGAGACTGACAAATCTAGAAACAATATTTTGTATAAATATGGAACACATGAAATATGTGTTTGGATCTGAAGGGCTTGAGCCTGGAATGATTCTCTTGACAAAATTGAGCGAGATGAGATTGTGCGCTCTCTTCAGACTAATAAGCATCTGGAATGGTCCTGCTCCAAATGCAGTGTTTTATAATCTTAAAGTTTTGGCAGTTTGCAAGTGTACAAAACTGAAAAGTCTGTTCCCATTCGATGTGGCTCGATCTCTTGTGCAATTGGAAGACCTCTCTGTAGAGCATTGCCCTAGCTTGGGCAGAGTTATTGAAGCAAGCAACAGCTTGAACACATCCATTGGTTTTCTAGCATTGAAGAAATTAGTTTTAATAAATCTTCCAGAGCTTACAGGGTTCTGCACCGAAGATGGTACTGTTGATACCGAGTGCCCTTCGTTGGAATACCTGTATCTGGATAAGTGCCCCCAGTTTATAAACTCTGCTTCTGCTTCTGTTAAACTCAACGATCAACAACATTTGAATTCTCTAGTGAAAAGATGGGAGGATATGTATAACCTATAA

Protein Analysis

1313

Amino Acids

148.66

Weight (kDa)

5.74

Isoelectric Point (pI)

44.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 15 - 188 1.2e-43 TIR domain
TIR_2 PF13676 18 - 113 2.7e-11 TIR domain
TIR PF01582 253 - 422 6.2e-52 TIR domain
TIR_2 PF13676 255 - 352 3.5e-15 TIR domain
NB-ARC PF00931 434 - 514 6.5e-09 NB-ARC domain
LRR_8 PF13855 794 - 850 1.2e-06 Leucine rich repeat
LRR_14 PF23598 856 - 919 2.4e-07 Leucine-rich repeat region
LRR_RPS2 PF23247 1079 - 1223 5e-25 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 3561, 3854
AccB1I GGYRCC 2 cut(s) 313, 3162
AccB7I CCANNNNNTGG 1 cut(s) 2224
AccI GTMKAC 1 cut(s) 2316
AccII CGCG 1 cut(s) 478
AccIII TCCGGA 1 cut(s) 2956
AciI CCGC 4 cut(s) 6, 82, 1046, 1347
AcoI YGGCCR 4 cut(s) 3, 97, 2226, 2294
AcuI CTGAAG 6 cut(s) 705, 2081, 2268, 2609, 3468, 3496
AdeI CACNNNGTG 2 cut(s) 255, 1268
AfiI CCNNNNNNNGG 9 cut(s) 164, 1733, 2224, 2580, 2629, 3676, 3677, 3817, 3832
AflII CTTAAG 3 cut(s) 872, 2627, 3107
AflIII ACRYGT 2 cut(s) 1399, 1660
AjnI CCWGG 3 cut(s) 84, 428, 3460
AjuI GAANNNNNNNTTGG 6 cut(s) 1647, 1679, 1997, 2029, 3279, 3311
AloI GAACNNNNNNTCC 2 cut(s) 1187, 1219
Alw21I GWGCWC 4 cut(s) 115, 270, 649, 1411
Alw26I GTCTC 5 cut(s) 419, 1793, 2121, 3306, 3376
Alw44I GTGCAC 1 cut(s) 1407
AlwNI CAGNNNCTG 1 cut(s) 3385
Aor13HI TCCGGA 1 cut(s) 2956
AoxI GGCC 6 cut(s) 3, 97, 1089, 2226, 2294, 2909
ApaLI GTGCAC 1 cut(s) 1407
ApeKI GCWGC 6 cut(s) 1811, 1901, 2025, 2398, 2876, 3194
AseI ATTAAT 1 cut(s) 3018
Asp700I GAANNNNTTC 2 cut(s) 1254, 1703
AspLEI GCGC 2 cut(s) 1493, 3506
AspS9I GGNCC 4 cut(s) 71, 1606, 2909, 3536
AsuC2I CCSGG 2 cut(s) 159, 2842
AsuHPI GGTGA 8 cut(s) 794, 1147, 1336, 1405, 1606, 2512, 3061, 3296
AvaII GGWCC 3 cut(s) 71, 1606, 3536
BaeGI GKGCMC 5 cut(s) 318, 1411, 3167, 3812, 3845
BalI TGGCCA 3 cut(s) 99, 2228, 2296
BamHI GGATCC 2 cut(s) 1048, 3205
BanI GGYRCC 2 cut(s) 313, 3162
BanII GRGCYC 3 cut(s) 115, 270, 649
BarI GAAGNNNNNNTAC 2 cut(s) 38, 70
BbsI GAAGAC 5 cut(s) 46, 82, 621, 1230, 3654
Bbv12I GWGCWC 4 cut(s) 115, 270, 649, 1411
BbvCI CCTCAGC 1 cut(s) 905
BbvI GCAGC 6 cut(s) 1798, 1913, 2012, 2385, 2888, 3206
BccI CCATC 4 cut(s) 108, 2174, 3782, 3912
BciT130I CCWGG 3 cut(s) 86, 430, 3462
BciVI GTATCC 1 cut(s) 1012
BclI TGATCA 2 cut(s) 1435, 2980
BcnI CCSGG 2 cut(s) 159, 2842
BcoDI GTCTC 5 cut(s) 419, 1793, 2121, 3306, 3376
BfaI CTAG 7 cut(s) 408, 1572, 3071, 3395, 3672, 3728, 3908
BfmI CTRYAG 1 cut(s) 3657
BfrI CTTAAG 3 cut(s) 872, 2627, 3107
BfuI GTATCC 1 cut(s) 1012
BglII AGATCT 1 cut(s) 2947
BisI GCNGC 7 cut(s) 6, 1812, 1902, 2026, 2399, 2877, 3195
BlsI GCNGC 7 cut(s) 7, 1813, 1903, 2027, 2400, 2878, 3196
BmcAI AGTACT 2 cut(s) 1254, 2496
Bme1390I CCNGG 5 cut(s) 86, 159, 430, 2842, 3462
Bme18I GGWCC 3 cut(s) 71, 1606, 3536
BmgT120I GGNCC 4 cut(s) 71, 1606, 2909, 3536
BmiI GGNNCC 6 cut(s) 169, 315, 755, 1050, 3164, 3207
BmrFI CCNGG 5 cut(s) 86, 159, 430, 2842, 3462
BmrI ACTGGG 3 cut(s) 2782, 3184, 3841
BmuI ACTGGG 3 cut(s) 2782, 3184, 3841
BpiI GAAGAC 5 cut(s) 46, 82, 621, 1230, 3654
Bpu10I CCTNAGC 1 cut(s) 905
BpuEI CTTGAG 6 cut(s) 1890, 2008, 3125, 3251, 3299, 3476
BpuMI CCSGG 2 cut(s) 159, 2842
Bsa29I ATCGAT 1 cut(s) 144
BsaAI YACGTR 1 cut(s) 1837
BsaI GGTCTC 3 cut(s) 419, 1793, 3306
BsaJI CCNNGG 4 cut(s) 1305, 1389, 1602, 2142
BsaWI WCCGGW 1 cut(s) 2956
BsaXI ACNNNNNCTCC 3 cut(s) 3246, 3276, 3914
Bsc4I CCNNNNNNNGG 9 cut(s) 164, 1733, 2224, 2580, 2629, 3676, 3677, 3817, 3832
Bse118I RCCGGY 1 cut(s) 2813
Bse1I ACTGG 6 cut(s) 2488, 2689, 2742, 2777, 3179, 3847
Bse3DI GCAATG 2 cut(s) 2111, 3664
BseAI TCCGGA 1 cut(s) 2956
BseBI CCWGG 3 cut(s) 86, 430, 3462
BseCI ATCGAT 1 cut(s) 144
BseDI CCNNGG 4 cut(s) 1305, 1389, 1602, 2142
BseLI CCNNNNNNNGG 9 cut(s) 164, 1733, 2224, 2580, 2629, 3676, 3677, 3817, 3832
BseMI GCAATG 2 cut(s) 2111, 3664
BseMII CTCAG 6 cut(s) 73, 761, 896, 2334, 2620, 3159
BseNI ACTGG 6 cut(s) 2488, 2689, 2742, 2777, 3179, 3847
BseRI GAGGAG 3 cut(s) 735, 1383, 3254
BseSI GKGCMC 5 cut(s) 318, 1411, 3167, 3812, 3845
BseXI GCAGC 6 cut(s) 1798, 1913, 2012, 2385, 2888, 3206
BseYI CCCAGC 1 cut(s) 468
BsgI GTGCAG 2 cut(s) 423, 3763
Bsh1236I CGCG 1 cut(s) 478
BshFI GGCC 6 cut(s) 5, 99, 1091, 2228, 2296, 2911
BshNI GGYRCC 2 cut(s) 313, 3162
BshVI ATCGAT 1 cut(s) 144
BsiHKAI GWGCWC 4 cut(s) 115, 270, 649, 1411
BsiSI CCGG 4 cut(s) 159, 2814, 2842, 2957
BslFI GGGAC 3 cut(s) 485, 1916, 2824
BslI CCNNNNNNNGG 9 cut(s) 164, 1733, 2224, 2580, 2629, 3676, 3677, 3817, 3832
BsmAI GTCTC 5 cut(s) 419, 1793, 2121, 3306, 3376
BsmFI GGGAC 3 cut(s) 485, 1916, 2824
BsmI GAATGC 2 cut(s) 290, 3358
BsnI GGCC 6 cut(s) 5, 99, 1091, 2228, 2296, 2911
Bso31I GGTCTC 3 cut(s) 419, 1793, 3306
Bsp1286I GDGCHC 8 cut(s) 115, 270, 318, 649, 1411, 3167, 3812, 3845
Bsp13I TCCGGA 1 cut(s) 2956
Bsp1407I TGTACA 3 cut(s) 105, 2748, 3590
Bsp19I CCATGG 2 cut(s) 1305, 1389
Bsp68I TCGCGA 1 cut(s) 478
BspACI CCGC 4 cut(s) 6, 82, 1046, 1347
BspANI GGCC 6 cut(s) 5, 99, 1091, 2228, 2296, 2911
BspCNI CTCAG 6 cut(s) 72, 760, 897, 2333, 2619, 3160
BspDI ATCGAT 1 cut(s) 144
BspEI TCCGGA 1 cut(s) 2956
BspFNI CGCG 1 cut(s) 478
BspHI TCATGA 2 cut(s) 1707, 2308
BspLI GGNNCC 6 cut(s) 169, 315, 755, 1050, 3164, 3207
BspT107I GGYRCC 2 cut(s) 313, 3162
BspTI CTTAAG 3 cut(s) 872, 2627, 3107
BspTNI GGTCTC 3 cut(s) 419, 1793, 3306
BsrDI GCAATG 2 cut(s) 2111, 3664
BsrFI RCCGGY 1 cut(s) 2813
BsrGI TGTACA 3 cut(s) 105, 2748, 3590
BsrI ACTGG 6 cut(s) 2488, 2689, 2742, 2777, 3179, 3847
BssAI RCCGGY 1 cut(s) 2813
BssECI CCNNGG 4 cut(s) 1305, 1389, 1602, 2142
BssT1I CCWWGG 4 cut(s) 1305, 1389, 1602, 2142
Bst2UI CCWGG 3 cut(s) 86, 430, 3462
Bst4CI ACNGT 4 cut(s) 2663, 2748, 2785, 3796
Bst6I CTCTTC 5 cut(s) 1360, 1752, 2509, 3174, 3515
BstAFI CTTAAG 3 cut(s) 872, 2627, 3107
BstAUI TGTACA 3 cut(s) 105, 2748, 3590
BstBAI YACGTR 1 cut(s) 1837
BstC8I GCNNGC 3 cut(s) 2815, 2881, 3697
BstDSI CCRYGG 2 cut(s) 1305, 1389
BstFNI CGCG 1 cut(s) 478
BstHHI GCGC 2 cut(s) 1493, 3506
BstMAI GTCTC 5 cut(s) 419, 1793, 2121, 3306, 3376
BstMWI GCNNNNNNNGC 4 cut(s) 670, 965, 1011, 3548
BstNI CCWGG 3 cut(s) 86, 430, 3462
BstNSI RCATGY 3 cut(s) 1403, 1664, 2032
BstSCI CCNGG 5 cut(s) 84, 157, 428, 2840, 3460
BstSFI CTRYAG 1 cut(s) 3657
BstSLI GKGCMC 5 cut(s) 318, 1411, 3167, 3812, 3845
BstUI CGCG 1 cut(s) 478
BstV1I GCAGC 6 cut(s) 1798, 1913, 2012, 2385, 2888, 3206
BstV2I GAAGAC 5 cut(s) 46, 82, 621, 1230, 3654
BstX2I RGATCY 8 cut(s) 154, 334, 457, 1048, 1736, 2947, 3205, 3443
BstYI RGATCY 8 cut(s) 154, 334, 457, 1048, 1736, 2947, 3205, 3443
Bsu15I ATCGAT 1 cut(s) 144
BsuI GTATCC 1 cut(s) 1012
BsuRI GGCC 6 cut(s) 5, 99, 1091, 2228, 2296, 2911
BsuTUI ATCGAT 1 cut(s) 144
BtgI CCRYGG 2 cut(s) 1305, 1389
BtsI GCAGTG 2 cut(s) 1148, 3558
BtsIMutI CAGTG 4 cut(s) 755, 1148, 2682, 3558
BtuMI TCGCGA 1 cut(s) 478
Cac8I GCNNGC 3 cut(s) 2815, 2881, 3697
CaiI CAGNNNCTG 1 cut(s) 3385
CciI TCATGA 2 cut(s) 1707, 2308
CfoI GCGC 2 cut(s) 1493, 3506
Cfr10I RCCGGY 1 cut(s) 2813
Cfr13I GGNCC 4 cut(s) 71, 1606, 2909, 3536
ClaI ATCGAT 1 cut(s) 144
CseI GACGC 1 cut(s) 503
CspCI CAANNNNNGTGG 2 cut(s) 3354, 3389
DraI TTTAAA 1 cut(s) 1960
DraIII CACNNNGTG 2 cut(s) 255, 1268
EaeI YGGCCR 4 cut(s) 3, 97, 2226, 2294
Eam1104I CTCTTC 5 cut(s) 1360, 1752, 2509, 3174, 3515
EarI CTCTTC 5 cut(s) 1360, 1752, 2509, 3174, 3515
Ecl136II GAGCTC 3 cut(s) 113, 268, 647
Eco130I CCWWGG 4 cut(s) 1305, 1389, 1602, 2142
Eco24I GRGCYC 3 cut(s) 115, 270, 649
Eco31I GGTCTC 3 cut(s) 419, 1793, 3306
Eco32I GATATC 3 cut(s) 1067, 2050, 2721
Eco47I GGWCC 3 cut(s) 71, 1606, 3536
Eco53kI GAGCTC 3 cut(s) 113, 268, 647
Eco57I CTGAAG 6 cut(s) 705, 2081, 2268, 2609, 3468, 3496
EcoICRI GAGCTC 3 cut(s) 113, 268, 647
EcoO109I RGGNCCY 1 cut(s) 1606
EcoRI GAATTC 3 cut(s) 1566, 2618, 3901
EcoRII CCWGG 3 cut(s) 84, 428, 3460
EcoRV GATATC 3 cut(s) 1067, 2050, 2721
EcoT14I CCWWGG 4 cut(s) 1305, 1389, 1602, 2142
EcoT22I ATGCAT 6 cut(s) 290, 1007, 1921, 2198, 2208, 2939
EcoT38I GRGCYC 3 cut(s) 115, 270, 649
ErhI CCWWGG 4 cut(s) 1305, 1389, 1602, 2142
FalI AAGNNNNNCTT 6 cut(s) 350, 382, 1239, 1271, 2079, 2111
FaqI GGGAC 3 cut(s) 485, 1916, 2824
FauI CCCGC 1 cut(s) 89
FauNDI CATATG 2 cut(s) 396, 2933
FbaI TGATCA 2 cut(s) 1435, 2980
FblI GTMKAC 1 cut(s) 2316
Fnu4HI GCNGC 7 cut(s) 6, 1812, 1902, 2026, 2399, 2877, 3195
FriOI GRGCYC 3 cut(s) 115, 270, 649
Fsp4HI GCNGC 7 cut(s) 6, 1812, 1902, 2026, 2399, 2877, 3195
FspBI CTAG 7 cut(s) 408, 1572, 3071, 3395, 3672, 3728, 3908
GlaI GCGC 2 cut(s) 1492, 3505
GluI GCNGC 7 cut(s) 6, 1812, 1902, 2026, 2399, 2877, 3195
GsaI CCCAGC 1 cut(s) 472
HaeIII GGCC 6 cut(s) 5, 99, 1091, 2228, 2296, 2911
HapII CCGG 4 cut(s) 159, 2814, 2842, 2957
HgaI GACGC 1 cut(s) 503
HhaI GCGC 2 cut(s) 1493, 3506
Hin6I GCGC 2 cut(s) 1491, 3504
HinP1I GCGC 2 cut(s) 1491, 3504
HincII GTYRAC 1 cut(s) 681
HindII GTYRAC 1 cut(s) 681
HindIII AAGCTT 3 cut(s) 375, 2160, 2354
HpaII CCGG 4 cut(s) 159, 2814, 2842, 2957
HphI GGTGA 8 cut(s) 794, 1147, 1336, 1405, 1606, 2512, 3061, 3296
Hpy166II GTNNAC 8 cut(s) 536, 681, 1409, 2317, 2698, 2863, 3215, 3591
Hpy8I GTNNAC 8 cut(s) 536, 681, 1409, 2317, 2698, 2863, 3215, 3591
HpyCH4III ACNGT 4 cut(s) 2663, 2748, 2785, 3796
HpyCH4IV ACGT 3 cut(s) 1767, 1836, 3045
HpyF10VI GCNNNNNNNGC 4 cut(s) 670, 965, 1011, 3548
HpySE526I ACGT 3 cut(s) 1767, 1836, 3045
HspAI GCGC 2 cut(s) 1491, 3504
Kpn2I TCCGGA 1 cut(s) 2956
KroI GCCGGC 1 cut(s) 2813
KroNI GCCGGC 1 cut(s) 2815
Ksp22I TGATCA 2 cut(s) 1435, 2980
LmnI GCTCC 4 cut(s) 173, 652, 748, 3547
Lsp1109I GCAGC 6 cut(s) 1798, 1913, 2012, 2385, 2888, 3206
MaeI CTAG 7 cut(s) 408, 1572, 3071, 3395, 3672, 3728, 3908
MaeII ACGT 3 cut(s) 1767, 1836, 3045
MaeIII GTNAC 5 cut(s) 183, 2420, 2680, 3049, 3341
MfeI CAATTG 4 cut(s) 912, 2055, 2808, 3641
MflI RGATCY 8 cut(s) 154, 334, 457, 1048, 1736, 2947, 3205, 3443
MhlI GDGCHC 8 cut(s) 115, 270, 318, 649, 1411, 3167, 3812, 3845
MlsI TGGCCA 3 cut(s) 99, 2228, 2296
MluNI TGGCCA 3 cut(s) 99, 2228, 2296
MlyI GAGTC 4 cut(s) 467, 614, 2995, 3132
MmeI TCCRAC 4 cut(s) 402, 582, 674, 3798
Mox20I TGGCCA 3 cut(s) 99, 2228, 2296
Mph1103I ATGCAT 6 cut(s) 290, 1007, 1921, 2198, 2208, 2939
MroI TCCGGA 1 cut(s) 2956
MroNI GCCGGC 1 cut(s) 2813
MroXI GAANNNNTTC 2 cut(s) 1254, 1703
MscI TGGCCA 3 cut(s) 99, 2228, 2296
MslI CAYNNNNRTG 2 cut(s) 1404, 2201
Msp20I TGGCCA 3 cut(s) 99, 2228, 2296
MspCI CTTAAG 3 cut(s) 872, 2627, 3107
MspI CCGG 4 cut(s) 159, 2814, 2842, 2957
MspR9I CCNGG 5 cut(s) 86, 159, 430, 2842, 3462
MunI CAATTG 4 cut(s) 912, 2055, 2808, 3641
Mva1269I GAATGC 2 cut(s) 290, 3358
MvaI CCWGG 3 cut(s) 86, 430, 3462
MvnI CGCG 1 cut(s) 478
MwoI GCNNNNNNNGC 4 cut(s) 670, 965, 1011, 3548
NaeI GCCGGC 1 cut(s) 2815
NciI CCSGG 2 cut(s) 159, 2842
NcoI CCATGG 2 cut(s) 1305, 1389
NdeI CATATG 2 cut(s) 396, 2933
NgoMIV GCCGGC 1 cut(s) 2813
NlaIV GGNNCC 6 cut(s) 169, 315, 755, 1050, 3164, 3207
NmuCI GTSAC 3 cut(s) 2420, 2680, 3049
NruI TCGCGA 1 cut(s) 478
NsiI ATGCAT 6 cut(s) 290, 1007, 1921, 2198, 2208, 2939
NspI RCATGY 3 cut(s) 1403, 1664, 2032
PagI TCATGA 2 cut(s) 1707, 2308
PciI ACATGT 2 cut(s) 1399, 1660
PctI GAATGC 2 cut(s) 290, 3358
PdiI GCCGGC 1 cut(s) 2815
PdmI GAANNNNTTC 2 cut(s) 1254, 1703
PfeI GAWTC 9 cut(s) 203, 450, 704, 920, 1187, 1691, 1745, 2602, 3469
PflMI CCANNNNNTGG 1 cut(s) 2224
PfoI TCCNGGA 1 cut(s) 157
PkrI GCNGC 7 cut(s) 7, 1813, 1903, 2027, 2400, 2878, 3196
PleI GAGTC 4 cut(s) 467, 613, 2995, 3132
PpsI GAGTC 4 cut(s) 467, 613, 2995, 3132
Ppu21I YACGTR 1 cut(s) 1837
PpuMI RGGWCCY 1 cut(s) 1606
PscI ACATGT 2 cut(s) 1399, 1660
PshBI ATTAAT 1 cut(s) 3018
PsiI TTATAA 2 cut(s) 3561, 3854
Psp124BI GAGCTC 3 cut(s) 115, 270, 649
Psp5II RGGWCCY 1 cut(s) 1606
Psp6I CCWGG 3 cut(s) 84, 428, 3460
PspFI CCCAGC 1 cut(s) 468
PspGI CCWGG 3 cut(s) 84, 428, 3460
PspN4I GGNNCC 6 cut(s) 169, 315, 755, 1050, 3164, 3207
PspPI GGNCC 4 cut(s) 71, 1606, 2909, 3536
PspPPI RGGWCCY 1 cut(s) 1606
PstNI CAGNNNCTG 1 cut(s) 3385
PsuI RGATCY 8 cut(s) 154, 334, 457, 1048, 1736, 2947, 3205, 3443
RruI TCGCGA 1 cut(s) 478
RseI CAYNNNNRTG 2 cut(s) 1404, 2201
SacI GAGCTC 3 cut(s) 115, 270, 649
SatI GCNGC 7 cut(s) 6, 1812, 1902, 2026, 2399, 2877, 3195
Sau96I GGNCC 4 cut(s) 71, 1606, 2909, 3536
ScaI AGTACT 2 cut(s) 1254, 2496
SchI GAGTC 4 cut(s) 467, 614, 2995, 3132
ScrFI CCNGG 5 cut(s) 86, 159, 430, 2842, 3462
SduI GDGCHC 8 cut(s) 115, 270, 318, 649, 1411, 3167, 3812, 3845
SfcI CTRYAG 1 cut(s) 3657
SinI GGWCC 3 cut(s) 71, 1606, 3536
SmiMI CAYNNNNRTG 2 cut(s) 1404, 2201
SmlI CTYRAG 9 cut(s) 872, 1905, 1987, 2627, 3107, 3140, 3266, 3314, 3455
SmoI CTYRAG 9 cut(s) 872, 1905, 1987, 2627, 3107, 3140, 3266, 3314, 3455
SsiI CCGC 4 cut(s) 6, 82, 1046, 1347
SspI AATATT 1 cut(s) 3405
SspMI CTAG 7 cut(s) 408, 1572, 3071, 3395, 3672, 3728, 3908
SstI GAGCTC 3 cut(s) 115, 270, 649
StyD4I CCNGG 5 cut(s) 84, 157, 428, 2840, 3460
StyI CCWWGG 4 cut(s) 1305, 1389, 1602, 2142
TaaI ACNGT 4 cut(s) 2663, 2748, 2785, 3796
TaiI ACGT 3 cut(s) 1770, 1839, 3048
TatI WGTACW 7 cut(s) 105, 1252, 2494, 2748, 2780, 3075, 3590
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 9 cut(s) 203, 450, 704, 920, 1187, 1691, 1745, 2602, 3469
TscAI CASTG 4 cut(s) 755, 1148, 2689, 3558
TseFI GTSAC 3 cut(s) 2420, 2680, 3049
TseI GCWGC 6 cut(s) 1811, 1901, 2025, 2398, 2876, 3194
Tsp45I GTSAC 3 cut(s) 2420, 2680, 3049
TspGWI ACGGA 4 cut(s) 470, 2563, 2697, 3068
TspRI CASTG 4 cut(s) 755, 1148, 2689, 3558
Van91I CCANNNNNTGG 1 cut(s) 2224
Vha464I CTTAAG 3 cut(s) 872, 2627, 3107
VneI GTGCAC 1 cut(s) 1407
VpaK11BI GGWCC 3 cut(s) 71, 1606, 3536
VspI ATTAAT 1 cut(s) 3018
XbaI TCTAGA 1 cut(s) 3394
XceI RCATGY 3 cut(s) 1403, 1664, 2032
XcmI CCANNNNNNNNNTGG 3 cut(s) 92, 747, 1624
XmiI GTMKAC 1 cut(s) 2316
XmnI GAANNNNTTC 2 cut(s) 1254, 1703
XspI CTAG 7 cut(s) 408, 1572, 3071, 3395, 3672, 3728, 3908
ZrmI AGTACT 2 cut(s) 1254, 2496
Zsp2I ATGCAT 6 cut(s) 290, 1007, 1921, 2198, 2208, 2939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.