Rroxscaffold_4G00301730

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000085
Physical Location & Seq
Reverse (-)
22071686 .. 22075416
3731 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00301730.1

Sequence Viewer

Length: 3264 bp
ATGGCCTCAAGCTCCCACACAGCCTCTGCATCGCTTCCTTCATCGGCTCATTGTTGGAAGTATGATGTATTTTTGAGTTTCAGGGGTGTAGATACTCGGAAGGGTATTACATTTGAATTATATGATCGTCTGCAGAACAAAAGAGGAATTAAAACATTCATTGATGACAAAGCCCTGCCAGCAGGGGCTGTTATTTCTCCCACTCTCCTAACAGCAATTGAAGAATCAAGGTTAGCAGTTGTTGTTCTCTCCCAAAACTATGCTTCTTCTACTTGGTGTTTGGAGGAACTTGCAAAGATTTGTCAATGCATGAAAGACGAAAGCAGAATTCTTCCACTTTTCTATCATGTCGAACCTACTGATGTTCGATATCAGAAAAGGAGTTTTGAAGAAGCTTTCACCAAGCATGAAAACCTTAACCGACACAAATCAGAGAAGGTGCAGGAGTGGAGAGATGCTTTAGCACAAGTGGCAAATTTTTCTGGGTGGCATACACAAAATTATAAGACTGAAAGAGAACTTGCCGATGCCATTGTGGACTTTGTGTGCAGTATAGTACAACCAGTTGGAATCATCATGGGAGATTTTGAAGCATTTGAAGCAACAAGGGAAGCCATGGACAAGGTTACAAAAGCGCTAAAAGATGATGAGGTCTCAGCTGTTGGGGTCTATGGAATGGGAGGCGTTGGCAAGACAACCATGGTAAAACATGTTGGTAATCAAGCCCGCAAAAATGGGATTTTTCATCATGTGATTATGGCTGTCATGTCCCAAAGCCCTGACTTGACGGAAATTCAGCACACATTGGCAGAGCTGCTGGGGATTGATTTACAAGGGAAGTCACAAAGTGGAAGAGCCTCTGGATTGAACAAAGAGATAATGAGAAAAAATAAAATCCTTATAATCTTGGATGACATTTGGGAGAGAATAGAATTGTCAAGCATAGGGATTCCCAGCTACAAGGAACTTCAAAAATGCAATTCCAAAGTCCTACTCACAACAAGGATACAGAATGTTTGTCATGCCATGGAGTGCCAAGAAAAGATCACCCTTAATGTCCTCTCGGAAGAGGATTCTTGGACCTTATTTATGAGAAATGCAAGAAGGTCTTTCGAAACGACCATTTTCAGGGATATAGCAAGGAAGGTAGCTAGAGAATGTTGCGGTCTACCATTTGCATTGATAGCAGTTGCAAAGGCACTTGGAGATAAAGACCTGCGAGAATGGAAAAAGGCAGCTCAAAGACTAAACAACTCGCAGACTGCCAACCTTGACGATAAGGGAGATGCATCCCAATGTATAATGTTAAGCTATGATTACTTGAAAGATGAGGATTGCAAGTCATGCTTCTTGGTTTGTAGCCTATTTCCTGAAGACTATGACATCCCAATAGAAGACTTGTTTAGGTATGCAATCGGGAAAGGTGTGTTTCGAGATGCTGACACAATTGAAGAAGCTAGAGAAACACTACATATGGTGGTCACATACCTTAAGAATTCAAGCTTGCTTTTAGATAGTGGCAAAGAACTATGTGTAAGGATGCATGACGTCATTCGGGATACAGCCATGAAAATTGCACTATCTGTAGATGGTCATGGGTTTTTTGTAAAAGCTGGCTGCAGTTTGGAGGATTGGCCACGTCAATTACAAGAAGGCTACTCTGCGGTCTCACTAATGGGGAATGAAATCCGCACACTACCAGAAGAGTTGGTATGTCCAAAACTCCAGGTTTTATTACTACAAGATAATTGCCATATAGAAAAGTTCCCAGAGGCCTTCTTTCAAAGTTCCAATGAACTGAGGGTCTTAAATCTTAGCAACACTTGCATTTTATTATTACCCCAATCATTCAGTCTTCTAGCCAACCTCCAAGTTTTGCATTTAGATGATTTGAAGATCATAACTGACATTTCCATGCTTGGAAAACTGAAAAAACTTGAGATCCTTAGCATGAGAGAATCACACATTGGGGAATTTCCAAAAGAAATAGGAGATTTAACCAATCTAAGGATGTTGGATTTGACTGGTGGACATATTGTCACAATTCCATCAAAAGTTTTTTCAAAGTTGCATAAACTAGAAGAACTGTACATGCAATGTGGGTTTTGGGACTGGGGGCGTGAAGTTGAGGGAGGAGAAGGAGAAACCAATGCTGGCTTTGATGAGTTGACTAGCTTGCCATATTTAAGAGTTTTGAAGGTTTGGATCTCCCATGCAAATTGTATCCCTAAAAATGTTGAACTCAACCCGAATTGGGTTGAGTTTGATATATGTATTGGAAGAGATGACGAGACGGTGGTCTCTCAATACTGTTATAATTCAGTATGCTTGACACTTGACACAGCCATCAGCACATTACCGGATTGGTTTGTCTTTGTGGTAATAAACAAAGCAGAGAAGCTAGAGTGGGTAGAGTGCAAAGGGTTGATTGATTTTTTTGTGGAATATGAGCATAGGAGGTTAGATAGATTGAAGTATCTCTCTATAATTGGTTGCGATGAGAACTTGAAAGAGTTGATATGGGTTCCGAAAGAACCTGTGTTTGAGAACTTGGAAGACTTGCATCTCAGTCGAGTAGATTTCATGAAAGAGTTATGTCTTGGTGATTTACCACAAGGGTCTCTATTCAGTCTCAAGTTACTTGAGGTTGACTCTTGTTATAACTGGGGGAACATACTTTTACCATCAAAATTGTTGCAGAGAGTACCAAATCTGGAAACTCTAATTTGTAATGATGTTGATGGAATGGAATATGTATTTGGATGCGAAGGATTTGAGCCGGAAAAATCAAAATTGATAGACTTGTCATTGAGGAGTTTAAATGCAGTCAGAAGCATATGTAATGGTCCTGCTCCACCTGCAATGTTCCAGACTCTTAAAGTTGTGTCCATTGAACAATGCAGCTTTGTAGGAAGTCTTTTCTCTTATGAGGCAGCTCAGTGTCTGTATCAATTGGAAGACCTTTCAGTTGGCAGTTGCCCTCTTTTGGAAAGAGTGATTGAAGCAAACAAGGAAACAATGAACAAGAAGAAGACCGTTTTTCCAAAATTGAAGAACTTAGTTTTGATGAGACTTCCTATGTTGTACAGTGGAAGTGCTACTATTGATTTTGAGTGTCCTTTACTGGAATGTTTGTATTTGAAGGACTGCTCCAAGTTTTTATCTTCAACCTCTGCTTCTGACTTCCACAGTAGGAAAAATGTCCAGTTCAATGATGAGCAACACCACCTATCTCTATGGGAGAGAATGTGGGGAGACACATAA

Protein Analysis

1087

Amino Acids

123.4

Weight (kDa)

5.35

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 20 - 182 3.7e-47 TIR domain
TIR_2 PF13676 23 - 119 7.3e-13 TIR domain
NB-ARC PF00931 204 - 367 7.8e-32 NB-ARC domain
LRR_8 PF13855 573 - 629 1.2e-06 Leucine rich repeat
LRR_14 PF23598 637 - 734 2.7e-10 Leucine-rich repeat region
LRR_RPS2 PF23247 851 - 993 3.1e-20 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 933 - 1052 2.9e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 504, 902, 2316, 2661
AarI CACCTGC 1 cut(s) 2866
AatII GACGTC 1 cut(s) 1551
Acc36I ACCTGC 2 cut(s) 1224, 2866
AccI GTMKAC 1 cut(s) 1168
AciI CCGC 4 cut(s) 727, 1164, 1664, 1690
AclWI GGATC 2 cut(s) 1936, 2213
AcoI YGGCCR 1 cut(s) 1634
AcsI RAATTY 5 cut(s) 327, 475, 792, 1495, 1973
AcuI CTGAAG 1 cut(s) 1392
AcyI GRCGYC 1 cut(s) 1548
AdeI CACNNNGTG 1 cut(s) 848
AfaI GTAC 4 cut(s) 558, 2090, 2706, 3086
AfeI AGCGCT 1 cut(s) 636
AfiI CCNNNNNNNGG 4 cut(s) 1128, 2007, 2254, 2986
AflII CTTAAG 1 cut(s) 1490
AflIII ACRYGT 1 cut(s) 709
AhdI GACNNNNNGTC 1 cut(s) 2036
AjiI CACGTC 1 cut(s) 1640
AjnI CCWGG 1 cut(s) 1725
AjuI GAANNNNNNNTTGG 4 cut(s) 1950, 1982, 2742, 2774
Alw26I GTCTC 8 cut(s) 658, 1672, 2285, 2305, 2625, 2636, 3064, 3249
AlwI GGATC 2 cut(s) 1936, 2213
AlwNI CAGNNNCTG 3 cut(s) 26, 188, 2944
Aor51HI AGCGCT 1 cut(s) 636
AoxI GGCC 3 cut(s) 3, 1634, 1773
ApeKI GCWGC 5 cut(s) 814, 1235, 1617, 2901, 2933
ApoI RAATTY 5 cut(s) 327, 475, 792, 1495, 1973
AspLEI GCGC 1 cut(s) 637
AspS9I GGNCC 2 cut(s) 1080, 2846
AsuHPI GGTGA 3 cut(s) 391, 1039, 2615
AsuII TTCGAA 1 cut(s) 1113
AvaII GGWCC 2 cut(s) 1080, 2846
BalI TGGCCA 1 cut(s) 1636
BbsI GAAGAC 6 cut(s) 1380, 1401, 1847, 2562, 2964, 3038
BbvI GCAGC 5 cut(s) 801, 1247, 1604, 2913, 2945
BccI CCATC 5 cut(s) 1583, 2056, 2354, 2692, 2735
BcgI CGANNNNNNTGC 2 cut(s) 2552, 2586
BciT130I CCWGG 1 cut(s) 1727
BciVI GTATCC 3 cut(s) 999, 1552, 2234
BcoDI GTCTC 8 cut(s) 658, 1672, 2285, 2305, 2625, 2636, 3064, 3249
BfaI CTAG 6 cut(s) 1152, 1458, 1859, 2078, 2172, 2402
BfmI CTRYAG 3 cut(s) 131, 1584, 1618
BfoI RGCGCY 1 cut(s) 638
BfrI CTTAAG 1 cut(s) 1490
BfuAI ACCTGC 2 cut(s) 1224, 2866
BfuI GTATCC 3 cut(s) 999, 1552, 2234
BisI GCNGC 5 cut(s) 815, 1236, 1618, 2902, 2934
BlsI GCNGC 5 cut(s) 816, 1237, 1619, 2903, 2935
Bme1390I CCNGG 1 cut(s) 1727
Bme18I GGWCC 2 cut(s) 1080, 2846
BmeRI GACNNNNNGTC 1 cut(s) 2036
BmgBI CACGTC 1 cut(s) 1640
BmgT120I GGNCC 2 cut(s) 1080, 2846
BmiI GGNNCC 1 cut(s) 2526
BmrFI CCNGG 1 cut(s) 1727
BmrI ACTGGG 2 cut(s) 2122, 2674
BmsI GCATC 9 cut(s) 38, 445, 517, 1276, 1298, 1426, 1530, 2572, 2753
BmuI ACTGGG 2 cut(s) 2122, 2674
BoxI GACNNNNGTC 1 cut(s) 2297
BpiI GAAGAC 6 cut(s) 1380, 1401, 1847, 2562, 2964, 3038
BplI GAGNNNNNCTC 2 cut(s) 2636, 2668
BpmI CTGGAG 1 cut(s) 1709
Bpu10I CCTNAGC 1 cut(s) 1946
Bpu14I TTCGAA 1 cut(s) 1113
BpuEI CTTGAG 3 cut(s) 1958, 2618, 2663
BsaHI GRCGYC 1 cut(s) 1548
BsaI GGTCTC 4 cut(s) 658, 1672, 2305, 2625
BsaJI CCNNGG 3 cut(s) 615, 699, 1026
BsaWI WCCGGW 1 cut(s) 2359
BsaXI ACNNNNNCTCC 2 cut(s) 3233, 3263
Bsc4I CCNNNNNNNGG 4 cut(s) 1128, 2007, 2254, 2986
Bse1I ACTGG 6 cut(s) 563, 2029, 2117, 2669, 3129, 3205
Bse3DI GCAATG 2 cut(s) 2102, 2868
BseBI CCWGG 1 cut(s) 1727
BseDI CCNNGG 3 cut(s) 615, 699, 1026
BseGI GGATG 6 cut(s) 916, 1289, 1383, 1545, 2016, 2768
BseLI CCNNNNNNNGG 4 cut(s) 1128, 2007, 2254, 2986
BseMI GCAATG 2 cut(s) 2102, 2868
BseMII CTCAG 4 cut(s) 669, 1790, 2581, 2951
BseNI ACTGG 6 cut(s) 563, 2029, 2117, 2669, 3129, 3205
BseRI GAGGAG 2 cut(s) 2148, 2827
BseXI GCAGC 5 cut(s) 801, 1247, 1604, 2913, 2945
BseYI CCCAGC 2 cut(s) 817, 953
BsgI GTGCAG 2 cut(s) 461, 568
BshFI GGCC 3 cut(s) 5, 1636, 1775
BsiSI CCGG 2 cut(s) 2360, 2780
BslFI GGGAC 2 cut(s) 754, 2123
BslI CCNNNNNNNGG 4 cut(s) 1128, 2007, 2254, 2986
BsmAI GTCTC 8 cut(s) 658, 1672, 2285, 2305, 2625, 2636, 3064, 3249
BsmBI CGTCTC 1 cut(s) 2285
BsmFI GGGAC 2 cut(s) 754, 2123
BsnI GGCC 3 cut(s) 5, 1636, 1775
Bso31I GGTCTC 4 cut(s) 658, 1672, 2305, 2625
Bsp119I TTCGAA 1 cut(s) 1113
Bsp1407I TGTACA 2 cut(s) 2088, 3084
Bsp143I GATC 5 cut(s) 124, 1044, 1896, 1941, 2205
Bsp19I CCATGG 3 cut(s) 615, 699, 1026
BspACI CCGC 4 cut(s) 727, 1164, 1664, 1690
BspANI GGCC 3 cut(s) 5, 1636, 1775
BspCNI CTCAG 4 cut(s) 668, 1791, 2580, 2950
BspHI TCATGA 1 cut(s) 2583
BspLI GGNNCC 1 cut(s) 2526
BspMAI CTGCAG 2 cut(s) 135, 1622
BspMI ACCTGC 2 cut(s) 1224, 2866
BspPI GGATC 2 cut(s) 1936, 2213
BspQI GCTCTTC 1 cut(s) 847
BspT104I TTCGAA 1 cut(s) 1113
BspTI CTTAAG 1 cut(s) 1490
BspTNI GGTCTC 4 cut(s) 658, 1672, 2305, 2625
BsrDI GCAATG 2 cut(s) 2102, 2868
BsrGI TGTACA 2 cut(s) 2088, 3084
BsrI ACTGG 6 cut(s) 563, 2029, 2117, 2669, 3129, 3205
BssECI CCNNGG 3 cut(s) 615, 699, 1026
BssMI GATC 5 cut(s) 124, 1044, 1896, 1941, 2205
BssNI GRCGYC 1 cut(s) 1548
BssT1I CCWWGG 3 cut(s) 615, 699, 1026
Bst2UI CCWGG 1 cut(s) 1727
Bst4CI ACNGT 6 cut(s) 2088, 2296, 2312, 3037, 3089, 3191
Bst6I CTCTTC 4 cut(s) 847, 1062, 1698, 2275
BstACI GRCGYC 1 cut(s) 1548
BstAFI CTTAAG 1 cut(s) 1490
BstAPI GCANNNNNTGC 2 cut(s) 1344, 1824
BstAUI TGTACA 2 cut(s) 2088, 3084
BstBI TTCGAA 1 cut(s) 1113
BstC8I GCNNGC 6 cut(s) 180, 727, 1505, 1615, 2155, 2177
BstDEI CTNAG 8 cut(s) 655, 1799, 1814, 1946, 2006, 2567, 2937, 3058
BstDSI CCRYGG 3 cut(s) 615, 699, 1026
BstF5I GGATG 6 cut(s) 916, 1289, 1383, 1545, 2016, 2768
BstH2I RGCGCY 1 cut(s) 638
BstHHI GCGC 1 cut(s) 637
BstKTI GATC 5 cut(s) 127, 1047, 1899, 1944, 2208
BstMAI GTCTC 8 cut(s) 658, 1672, 2285, 2305, 2625, 2636, 3064, 3249
BstMBI GATC 5 cut(s) 124, 1044, 1896, 1941, 2205
BstMWI GCNNNNNNNGC 7 cut(s) 179, 470, 599, 1184, 1344, 1824, 2858
BstNI CCWGG 1 cut(s) 1727
BstNSI RCATGY 2 cut(s) 713, 2095
BstPAI GACNNNNGTC 1 cut(s) 2297
BstSCI CCNGG 1 cut(s) 1725
BstSFI CTRYAG 3 cut(s) 131, 1584, 1618
BstV1I GCAGC 5 cut(s) 801, 1247, 1604, 2913, 2945
BstV2I GAAGAC 6 cut(s) 1380, 1401, 1847, 2562, 2964, 3038
BstX2I RGATCY 2 cut(s) 1941, 2205
BstYI RGATCY 2 cut(s) 1941, 2205
BsuI GTATCC 3 cut(s) 999, 1552, 2234
BsuRI GGCC 3 cut(s) 5, 1636, 1775
BtgI CCRYGG 3 cut(s) 615, 699, 1026
BtgZI GCGATG 2 cut(s) 15, 2511
BtrI CACGTC 1 cut(s) 1640
BtsCI GGATG 6 cut(s) 916, 1289, 1383, 1545, 2016, 2768
BtsIMutI CAGTG 2 cut(s) 2945, 3094
BveI ACCTGC 2 cut(s) 1224, 2866
Cac8I GCNNGC 6 cut(s) 180, 727, 1505, 1615, 2155, 2177
CaiI CAGNNNCTG 3 cut(s) 26, 188, 2944
CciI TCATGA 1 cut(s) 2583
CfoI GCGC 1 cut(s) 637
Cfr13I GGNCC 2 cut(s) 1080, 2846
Csp6I GTAC 4 cut(s) 557, 2089, 2705, 3085
CviQI GTAC 4 cut(s) 557, 2089, 2705, 3085
DdeI CTNAG 8 cut(s) 655, 1799, 1814, 1946, 2006, 2567, 2937, 3058
DpnI GATC 5 cut(s) 126, 1046, 1898, 1943, 2207
DpnII GATC 5 cut(s) 124, 1044, 1896, 1941, 2205
DraI TTTAAA 1 cut(s) 2820
DraIII CACNNNGTG 1 cut(s) 848
DriI GACNNNNNGTC 1 cut(s) 2036
EaeI YGGCCR 1 cut(s) 1634
Eam1104I CTCTTC 4 cut(s) 847, 1062, 1698, 2275
Eam1105I GACNNNNNGTC 1 cut(s) 2036
EarI CTCTTC 4 cut(s) 847, 1062, 1698, 2275
Eco130I CCWWGG 3 cut(s) 615, 699, 1026
Eco147I AGGCCT 1 cut(s) 1775
Eco31I GGTCTC 4 cut(s) 658, 1672, 2305, 2625
Eco32I GATATC 1 cut(s) 371
Eco47I GGWCC 2 cut(s) 1080, 2846
Eco47III AGCGCT 1 cut(s) 636
Eco57I CTGAAG 1 cut(s) 1392
EcoRI GAATTC 2 cut(s) 327, 1495
EcoRII CCWGG 1 cut(s) 1725
EcoRV GATATC 1 cut(s) 371
EcoT14I CCWWGG 3 cut(s) 615, 699, 1026
EcoT22I ATGCAT 3 cut(s) 311, 1291, 1545
ErhI CCWWGG 3 cut(s) 615, 699, 1026
Esp3I CGTCTC 1 cut(s) 2285
FalI AAGNNNNNCTT 4 cut(s) 1093, 1125, 1331, 1363
FaqI GGGAC 2 cut(s) 754, 2123
FauI CCCGC 1 cut(s) 734
FauNDI CATATG 2 cut(s) 1473, 2837
FblI GTMKAC 1 cut(s) 1168
Fnu4HI GCNGC 5 cut(s) 815, 1236, 1618, 2902, 2934
FokI GGATG 6 cut(s) 923, 1276, 1370, 1552, 2023, 2775
Fsp4HI GCNGC 5 cut(s) 815, 1236, 1618, 2902, 2934
FspBI CTAG 6 cut(s) 1152, 1458, 1859, 2078, 2172, 2402
GlaI GCGC 1 cut(s) 636
GluI GCNGC 5 cut(s) 815, 1236, 1618, 2902, 2934
GsaI CCCAGC 2 cut(s) 821, 957
GsuI CTGGAG 1 cut(s) 1709
HaeII RGCGCY 1 cut(s) 638
HaeIII GGCC 3 cut(s) 5, 1636, 1775
HapII CCGG 2 cut(s) 2360, 2780
HhaI GCGC 1 cut(s) 637
Hin1I GRCGYC 1 cut(s) 1548
Hin6I GCGC 1 cut(s) 635
HinP1I GCGC 1 cut(s) 635
HincII GTYRAC 2 cut(s) 2169, 2650
HindII GTYRAC 2 cut(s) 2169, 2650
HindIII AAGCTT 2 cut(s) 393, 1501
HinfI GANTC 7 cut(s) 224, 570, 949, 1073, 1958, 2651, 2872
HpaII CCGG 2 cut(s) 2360, 2780
HphI GGTGA 3 cut(s) 391, 1039, 2615
Hpy166II GTNNAC 5 cut(s) 538, 1169, 2030, 2169, 2650
Hpy188I TCNGA 7 cut(s) 99, 375, 433, 1066, 2529, 2831, 3181
Hpy188III TCNNGA 8 cut(s) 861, 1370, 1417, 1433, 1556, 2584, 2714, 2869
Hpy8I GTNNAC 5 cut(s) 538, 1169, 2030, 2169, 2650
HpyCH4III ACNGT 6 cut(s) 2088, 2296, 2312, 3037, 3089, 3191
HpyCH4IV ACGT 2 cut(s) 1548, 1639
HpyF10VI GCNNNNNNNGC 7 cut(s) 179, 470, 599, 1184, 1344, 1824, 2858
HpyF3I CTNAG 8 cut(s) 655, 1799, 1814, 1946, 2006, 2567, 2937, 3058
HpySE526I ACGT 2 cut(s) 1548, 1639
Hsp92I GRCGYC 1 cut(s) 1548
HspAI GCGC 1 cut(s) 635
Kzo9I GATC 5 cut(s) 124, 1044, 1896, 1941, 2205
LguI GCTCTTC 1 cut(s) 847
LmnI GCTCC 3 cut(s) 17, 2857, 3155
Lsp1109I GCAGC 5 cut(s) 801, 1247, 1604, 2913, 2945
LweI GCATC 9 cut(s) 38, 445, 517, 1276, 1298, 1426, 1530, 2572, 2753
MaeI CTAG 6 cut(s) 1152, 1458, 1859, 2078, 2172, 2402
MaeII ACGT 2 cut(s) 1548, 1639
MaeIII GTNAC 5 cut(s) 625, 840, 1480, 2038, 2637
MalI GATC 5 cut(s) 126, 1046, 1898, 1943, 2207
MboI GATC 5 cut(s) 124, 1044, 1896, 1941, 2205
MfeI CAATTG 3 cut(s) 216, 1446, 2951
MflI RGATCY 2 cut(s) 1941, 2205
MlsI TGGCCA 1 cut(s) 1636
MluNI TGGCCA 1 cut(s) 1636
MlyI GAGTC 2 cut(s) 2645, 2866
MmeI TCCRAC 3 cut(s) 35, 547, 1995
Mox20I TGGCCA 1 cut(s) 1636
Mph1103I ATGCAT 3 cut(s) 311, 1291, 1545
MscI TGGCCA 1 cut(s) 1636
MslI CAYNNNNRTG 3 cut(s) 1294, 1884, 1913
Msp20I TGGCCA 1 cut(s) 1636
MspA1I CMGCKG 1 cut(s) 659
MspCI CTTAAG 1 cut(s) 1490
MspI CCGG 2 cut(s) 2360, 2780
MspR9I CCNGG 1 cut(s) 1727
MunI CAATTG 3 cut(s) 216, 1446, 2951
MvaI CCWGG 1 cut(s) 1727
MwoI GCNNNNNNNGC 7 cut(s) 179, 470, 599, 1184, 1344, 1824, 2858
NcoI CCATGG 3 cut(s) 615, 699, 1026
NdeI CATATG 2 cut(s) 1473, 2837
NdeII GATC 5 cut(s) 124, 1044, 1896, 1941, 2205
NlaIV GGNNCC 1 cut(s) 2526
NmuCI GTSAC 3 cut(s) 840, 1480, 2038
NsiI ATGCAT 3 cut(s) 311, 1291, 1545
NspI RCATGY 2 cut(s) 713, 2095
NspV TTCGAA 1 cut(s) 1113
PagI TCATGA 1 cut(s) 2583
PaqCI CACCTGC 1 cut(s) 2866
PceI AGGCCT 1 cut(s) 1775
PciI ACATGT 1 cut(s) 709
PciSI GCTCTTC 1 cut(s) 847
PfeI GAWTC 5 cut(s) 224, 570, 949, 1073, 1958
PkrI GCNGC 5 cut(s) 816, 1237, 1619, 2903, 2935
PleI GAGTC 2 cut(s) 2645, 2866
PpsI GAGTC 2 cut(s) 2645, 2866
PscI ACATGT 1 cut(s) 709
PshAI GACNNNNGTC 1 cut(s) 2297
PsiI TTATAA 4 cut(s) 504, 902, 2316, 2661
Psp6I CCWGG 1 cut(s) 1725
PspFI CCCAGC 2 cut(s) 817, 953
PspGI CCWGG 1 cut(s) 1725
PspN4I GGNNCC 1 cut(s) 2526
PspPI GGNCC 2 cut(s) 1080, 2846
PstI CTGCAG 2 cut(s) 135, 1622
PstNI CAGNNNCTG 3 cut(s) 26, 188, 2944
PsuI RGATCY 2 cut(s) 1941, 2205
PvuII CAGCTG 1 cut(s) 659
RsaI GTAC 4 cut(s) 558, 2090, 2706, 3086
RsaNI GTAC 4 cut(s) 557, 2089, 2705, 3085
RseI CAYNNNNRTG 3 cut(s) 1294, 1884, 1913
SapI GCTCTTC 1 cut(s) 847
SatI GCNGC 5 cut(s) 815, 1236, 1618, 2902, 2934
Sau3AI GATC 5 cut(s) 124, 1044, 1896, 1941, 2205
Sau96I GGNCC 2 cut(s) 1080, 2846
SchI GAGTC 2 cut(s) 2645, 2866
ScrFI CCNGG 1 cut(s) 1727
SfaNI GCATC 9 cut(s) 38, 445, 517, 1276, 1298, 1426, 1530, 2572, 2753
SfcI CTRYAG 3 cut(s) 131, 1584, 1618
SfuI TTCGAA 1 cut(s) 1113
SinI GGWCC 2 cut(s) 1080, 2846
SmiMI CAYNNNNRTG 3 cut(s) 1294, 1884, 1913
SmlI CTYRAG 5 cut(s) 7, 1490, 1937, 2633, 2642
SmoI CTYRAG 5 cut(s) 7, 1490, 1937, 2633, 2642
SseBI AGGCCT 1 cut(s) 1775
SsiI CCGC 4 cut(s) 727, 1164, 1664, 1690
SspMI CTAG 6 cut(s) 1152, 1458, 1859, 2078, 2172, 2402
StuI AGGCCT 1 cut(s) 1775
StyD4I CCNGG 1 cut(s) 1725
StyI CCWWGG 3 cut(s) 615, 699, 1026
TaaI ACNGT 6 cut(s) 2088, 2296, 2312, 3037, 3089, 3191
TaiI ACGT 2 cut(s) 1551, 1642
TaqI TCGA 5 cut(s) 351, 367, 1113, 1432, 2572
TatI WGTACW 3 cut(s) 556, 2088, 3084
TfiI GAWTC 5 cut(s) 224, 570, 949, 1073, 1958
TscAI CASTG 2 cut(s) 2945, 3094
TseFI GTSAC 3 cut(s) 840, 1480, 2038
TseI GCWGC 5 cut(s) 814, 1235, 1617, 2901, 2933
Tsp45I GTSAC 3 cut(s) 840, 1480, 2038
TspGWI ACGGA 1 cut(s) 803
TspRI CASTG 2 cut(s) 2945, 3094
Vha464I CTTAAG 1 cut(s) 1490
VpaK11BI GGWCC 2 cut(s) 1080, 2846
XapI RAATTY 5 cut(s) 327, 475, 792, 1495, 1973
XceI RCATGY 2 cut(s) 713, 2095
XmiI GTMKAC 1 cut(s) 1168
XspI CTAG 6 cut(s) 1152, 1458, 1859, 2078, 2172, 2402
ZraI GACGTC 1 cut(s) 1549
Zsp2I ATGCAT 3 cut(s) 311, 1291, 1545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.