RchiOBHm_Chr3g0473341

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000085
Physical Location & Seq
Forward (+)
19208450 .. 19214085
5636 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43902

Sequence Viewer

Length: 3693 bp
ATGGAGTTTTATCAGTATGTCTTTGAGACGATGAGGATGATTATTGTGATTAGTGCGGTAATGGTGCAATGGTTCGGTGGCATAGCCTGCCTTAAGTGGATATGGAAGAATTGGTTCATTATTGTGGTGATTGTGACATGGAACGTAATAGCCAGTCTTGGGTGGATATGGAAGAGTAGGTTCAGTTACACCTTTGAAACCCCAGAGCAGATATCAGAGGGTAATCAGGAACTGGTTGACGTCGTTGAAACCCCAGAGCCAATATCAGAGGATCATCAAGAACTGCTTGACGTCGTTGAAACCCCAGATCCAATATCAGAGGATCATCAAGAACCGGTTGACGTCGACGCCTCTTCATCATCAGCTGAATCACCAGCTCCTACGTGGAAGCATGATGTGTTTTTGAGCTTCAGGGGTGAAGATACTCGTAAGAGCATCACATTCTCTTTATACAATCGACTGCAAAAAAGGAGAATCAAAACATTCATGGATGACCCAGATCTTCAACGAGGGGATGTTATTTCTTCCGCTCTGCCAACGGCAATTAAAGAATCAAGGTTTGCAATTGTTGTTCTCTCTCAAAACTATGCCTCTTCCACTTGGTGTTTGGAGGAACTGAGACACATTTGTGAATGCATGAAAGGAGACAACAATAGAATTCTACCACTTTTTTATAATGTGGATCCTACTGATGTAAGACGTCAGAAGAGCAGTTTCGGAGATGCTTTAACTAAGCATGAAAATTCTAGGCGACACGGAGAGAAGGTGCAGCAGTGGAGAGATGCTTTAACAAAGGTGGCAAATTTCGCCGGGTGGCATTCAGCGACATTTAAAAATGACGAAGAACTTTTGGTTCAAATTGTGGAAGAGGTGTGCAATAAATTACGACCGACTGAACCTGTGTTAAAAGTGTCCCTCGGAGATTTTGAAGAATTTGCAGCAACAAAAGAAGCCATTGATAAGGTCATGGAGGCGCTAAAAGATGGTGAGGCCACTACCATTGGAGTCTACGGCATGGGGGGCGTTGGAAAGACAGCAATGGTGAAACATGTAGGTGTACAAGCCGAAGAAAAAAAGCTTTTTAATAAAGCGATTATGGCTGCCGTATCCCAACAACCCGACTTGAAGAAAATTCAACAGGAATTTGCAGAAACGCTGGGCTTGGAATTCAAGGGGACGACGGAAATTGTAAGAGCCCGAGAATTGAAGGAGAAAATAATGAGAGAAACCGGGATCCTCATAATCTTGGATGACATTTGGCAGACAATAAAGTTTTCAGACATTGGAATTCCGAGCCACAAGGAACTTCAAACATGCAATTCCAAAGTCCTAATGACCACCAGAAGTTTCAAAGTTTGCCATTCCATGGGGAGCCATGCAAAAATTCCTCTCAATACCTTATCAAAAGGAGATTCTTGGCGCTTATTTGTGAGGGAAGCGGGGATTTCTTCTGACAAATCTTCCAATTTCTGTGATGTAGCGAGTGAGGTGGCAAGAGAATGCGCTGGTCTACCAATGGCTTTGATAGCAGTTGCGAGGGCCCTTCGAGGTGAAGGCTTGGATGGATGGAAGGAAGCTGCTCGACAACTAAAAGCGTCTCAACCTCCCATCCCTGAAGATGAGGGAGATGTGTTCAAATGCATAAAGTTAAGCTATGATTACTTGAAATCTAATGATTCCAAATCATGCTTCTTGCTTTGCTGCCTGTTCCCAGAAGATTATGATATCCCAATTGAATACTTGTTCAAGTATGGAATGTTTCAAGGTTCAAACTTGCTACGAGCCCGAGAGAGAACACATTCAGTGGTGAAGGCCCTTAAAGATTCTAGCTTGCTTTTAGACGGTAGATATGACGGACTTGTAAGGATGCATGATGTCATTCGGGATATGGCAATGCGAATTTCGTTATCTGAAGGCCCGCGGTTCTGGGTGATCAAAGATGGCTATGAAATGAAGAATTGGCCAAACTATGCGATCTCAATAAAGCATAACAGCAAGCTACCCAAAGAGTTGGTATGTCCAAATCTCCAGATTTTATTACTACAAGCCCGTTCTCTAAAGGAGATCCCAAAATATTTTTTCGCAAGTCAAAATGAATTAAGAGTCTTGGATCTTAGCTACAATGATATTTCATTACTACCCCAATCAATCAGTTTCCTAACCAACCTTCGAGCTTTGTATTTAGATCATTGTAAGAACATAACTGACATTTCTGTAATCGGAAAACTTCACAAGCTTGAGATTCTTAAAATGAAATCAAATGGTCTGAGAGAATTGCCAAGAGAAATAGGACAATTGACCAATCTAAGGATGCTGGATGTCAGTGTTAGTGAAAGTTTCTTTCCTAAAATCTTTTCTAGATACAGGGATGGATGTATTAGCACAATTCCATCTAAAGTGATATCAAAGTTGCATAAATTAGAAGAACTGTACATGCAATACTGTGGATTTTGGGGTTGGGGGAGTAAAATTGATGGAGAAGGAGAAGAAACTAATATTGGCTTTGATGAATTAGCTGGTTTATCATATTTAAGCATTTTGAAAGTTTGCATATCAGATGCAAATTGCATCCCTAAACGTGTTAAGGTCCAACCGAATTGGGATTACTTTGATATTAAAATTTCCAGCAGACAGAGGGACAATATGAAATTACAATCTGGTCATAATTGTAGAACCTTGAGTCTTGATAGCACTGACACAACCATAGGAACCTTTCCGGACTGGTTTGTCAACGCTGGGGTAAAGAATACTGAGAGGCTACGTTATGAAGAATGCAAAAATTTGAGTAACATTCTTGTGGAATATGACCGTGGGAAGTTACATGGACTGAAATTTCTCTCTGTAATTGGTCGGTGTGATAAGTTGAAAGAATTGATGGATGCAAATCATGCAATAACATGTGTTCCAGATATGCCTGTGTTTGAGAACTTAGAAGAGTTGTATCTAGAAAACCTGGGTGACCTGAAGAAGTTATGTGTTGGTGAGTTACCACCTGGATCTCTCCGCAGTCTGAAATTACTGGAGGTCAGTCGGTGTCCTAACTTGGGGAATGTACTGTTGCCATCAAAATTGTTGCAGAAACTACCAAATCTGGGAAAACTAATCTGTGAGACAATTGGAGTGGAATATGTGTTTGGATGTGAAGGATTTGAGCCAGATCAAACAAATCTGAGAGAGATGGAGTTGATGGATCTAGACGCAGTAATAAGCATATGTAATGGTCCTGCTCCACGTGGAATGTTCCAAGCTCTGAAGGAATTGGTCATTGACGGTTGCAACTTCCAGGGAAGCCTCTTCACATTTGATGTAGCTCAGTGTCTTTTTCAATTGGAAGACCTTTATGTATCCCACTGCCGTGTCTTGGAAAGAGTAATTGAAGCAAGGCGGGAAACAGTGAATAGCAAGAAGACCGTTCTTCCAAAATTGAAGAACTTATGTTTGGTATATCTTCCAAAGTTGTACGAAGGAAGTGCTACTATTGATTTTGAGTGTCCTTCATTAGAAAACTTGTGTCTAACGGGGTGCCCCCACTTGTCATTCCCATGCTCTGCTTCTGACTTCTTCCACAGCAGGAAGCAAGCCCTTTTCAATGATCCTCTTCTTGGGAATCGGATTCCCAAATTGATAGAGAAACAACTTCAGAATAGTATGAGACTGTGCCGAGGTTTGGAAGTCATATATGCCGAGCCGTGA

Protein Analysis

1230

Amino Acids

139.73

Weight (kDa)

6.02

Isoelectric Point (pI)

38.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 130 - 300 1.9e-52 TIR domain
TIR_2 PF13676 133 - 229 4.6e-16 TIR domain
NB-ARC PF00931 316 - 481 1.8e-27 NB-ARC domain
LRR_8 PF13855 674 - 732 1.7e-10 Leucine rich repeat
LRR_14 PF23598 676 - 882 3.4e-13 Leucine-rich repeat region
LRR_RPS2 PF23247 916 - 1035 2.3e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 977 - 1072 3.5e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1058 - 1178 1.6e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 675
AasI GACNNNNNNGTC 1 cut(s) 2726
AatII GACGTC 4 cut(s) 243, 294, 345, 703
AccB1I GGYRCC 1 cut(s) 3522
AccB7I CCANNNNNTGG 1 cut(s) 1366
AccBSI CCGCTC 1 cut(s) 530
AccI GTMKAC 3 cut(s) 345, 1008, 1510
AccII CGCG 1 cut(s) 1921
AccIII TCCGGA 1 cut(s) 2716
AciI CCGC 7 cut(s) 56, 528, 1439, 1919, 1921, 3004, 3385
AcoI YGGCCR 1 cut(s) 1961
AcuI CTGAAG 6 cut(s) 394, 1635, 1932, 2984, 3272, 3623
AcvI CACGTG 1 cut(s) 3233
AcyI GRCGYC 5 cut(s) 240, 291, 342, 348, 700
AdeI CACNNNGTG 1 cut(s) 603
AfaI GTAC 4 cut(s) 1059, 2432, 3054, 3461
AfiI CCNNNNNNNGG 8 cut(s) 159, 1366, 2307, 3044, 3092, 3361, 3602, 3667
AflII CTTAAG 1 cut(s) 92
AflIII ACRYGT 3 cut(s) 1048, 2578, 2897
AgeI ACCGGT 1 cut(s) 334
AjnI CCWGG 3 cut(s) 2952, 2992, 3282
AjuI GAANNNNNNNTTGG 8 cut(s) 1673, 1705, 2481, 2513, 3117, 3149, 3422, 3454
AleI CACNNNNGTG 2 cut(s) 627, 3354
Alw26I GTCTC 6 cut(s) 20, 613, 639, 1602, 3104, 3646
AlwNI CAGNNNCTG 1 cut(s) 232
Ama87I CYCGRG 2 cut(s) 1197, 1785
Aor13HI TCCGGA 1 cut(s) 2716
AoxI GGCC 5 cut(s) 990, 1539, 1812, 1915, 1961
ApaI GGGCCC 1 cut(s) 1543
ApeKI GCWGC 5 cut(s) 769, 938, 1100, 1577, 1701
AsiGI ACCGGT 1 cut(s) 334
Asp700I GAANNNNTTC 2 cut(s) 113, 1798
AspLEI GCGC 3 cut(s) 976, 1422, 1505
AspS9I GGNCC 6 cut(s) 1539, 1540, 1813, 1916, 2587, 3221
AsuC2I CCSGG 2 cut(s) 811, 1231
AvaI CYCGRG 2 cut(s) 1197, 1785
AvaII GGWCC 2 cut(s) 2587, 3221
BaeGI GKGCMC 2 cut(s) 1543, 3527
BalI TGGCCA 1 cut(s) 1963
BamHI GGATCC 2 cut(s) 682, 1233
BanI GGYRCC 1 cut(s) 3522
BanII GRGCYC 3 cut(s) 1198, 1543, 1786
BbrPI CACGTG 1 cut(s) 3233
BbsI GAAGAC 2 cut(s) 3339, 3413
BbvI GCAGC 5 cut(s) 781, 950, 1087, 1564, 1688
BceAI ACGGC 5 cut(s) 555, 1027, 1088, 3339, 3673
BcgI CGANNNNNNTGC 2 cut(s) 3452, 3486
BciT130I CCWGG 3 cut(s) 2954, 2994, 3284
BciVI GTATCC 2 cut(s) 1117, 3355
BclI TGATCA 1 cut(s) 1932
BcnI CCSGG 2 cut(s) 811, 1231
BcoDI GTCTC 6 cut(s) 20, 613, 639, 1602, 3104, 3646
BfaI CTAG 5 cut(s) 747, 1827, 2358, 2945, 3194
BfoI RGCGCY 2 cut(s) 977, 1423
BfrI CTTAAG 1 cut(s) 92
BfuI GTATCC 2 cut(s) 1117, 3355
BglII AGATCT 1 cut(s) 499
BisI GCNGC 5 cut(s) 770, 939, 1101, 1578, 1702
BlsI GCNGC 5 cut(s) 771, 940, 1102, 1579, 1703
Bme1390I CCNGG 5 cut(s) 811, 1231, 2954, 2994, 3284
Bme18I GGWCC 2 cut(s) 2587, 3221
BmeT110I CYCGRG 2 cut(s) 1197, 1785
BmgT120I GGNCC 6 cut(s) 1539, 1540, 1813, 1916, 2587, 3221
BmiI GGNNCC 6 cut(s) 684, 1235, 1373, 1541, 2710, 3524
BmrFI CCNGG 5 cut(s) 811, 1231, 2954, 2994, 3284
BmsI GCATC 8 cut(s) 444, 712, 772, 1857, 2301, 2548, 2577, 2869
BpiI GAAGAC 2 cut(s) 3339, 3413
BpmI CTGGAG 2 cut(s) 2012, 3041
BpuEI CTTGAG 2 cut(s) 2258, 2698
BpuMI CCSGG 2 cut(s) 811, 1231
BsaAI YACGTR 2 cut(s) 384, 3233
BsaBI GATNNNNATC 1 cut(s) 2883
BsaHI GRCGYC 5 cut(s) 240, 291, 342, 348, 700
BsaJI CCNNGG 7 cut(s) 916, 1365, 1919, 2809, 2953, 3283, 3661
BsaWI WCCGGW 2 cut(s) 334, 2716
BsaXI ACNNNNNCTCC 2 cut(s) 750, 780
Bsc4I CCNNNNNNNGG 8 cut(s) 159, 1366, 2307, 3044, 3092, 3361, 3602, 3667
Bse118I RCCGGY 1 cut(s) 334
Bse1I ACTGG 4 cut(s) 153, 237, 2726, 3024
Bse3DI GCAATG 3 cut(s) 74, 1044, 1899
Bse8I GATNNNNATC 1 cut(s) 2883
BseAI TCCGGA 1 cut(s) 2716
BseBI CCWGG 3 cut(s) 2954, 2994, 3284
BseDI CCNNGG 7 cut(s) 916, 1365, 1919, 2809, 2953, 3283, 3661
BseJI GATNNNNATC 1 cut(s) 2883
BseLI CCNNNNNNNGG 8 cut(s) 159, 1366, 2307, 3044, 3092, 3361, 3602, 3667
BseMI GCAATG 3 cut(s) 74, 1044, 1899
BseMII CTCAG 5 cut(s) 608, 2258, 2742, 3161, 3326
BseNI ACTGG 4 cut(s) 153, 237, 2726, 3024
BseSI GKGCMC 2 cut(s) 1543, 3527
BseXI GCAGC 5 cut(s) 781, 950, 1087, 1564, 1688
BseYI CCCAGC 2 cut(s) 1156, 2735
BsgI GTGCAG 1 cut(s) 788
Bsh1236I CGCG 1 cut(s) 1921
Bsh1285I CGRYCG 1 cut(s) 890
BshFI GGCC 5 cut(s) 992, 1541, 1814, 1917, 1963
BshNI GGYRCC 1 cut(s) 3522
BshTI ACCGGT 1 cut(s) 334
BsiEI CGRYCG 1 cut(s) 890
BsiHKCI CYCGRG 2 cut(s) 1197, 1785
BsiSI CCGG 4 cut(s) 335, 810, 1230, 2717
BslFI GGGAC 3 cut(s) 898, 1189, 2651
BslI CCNNNNNNNGG 8 cut(s) 159, 1366, 2307, 3044, 3092, 3361, 3602, 3667
BsmAI GTCTC 6 cut(s) 20, 613, 639, 1602, 3104, 3646
BsmBI CGTCTC 2 cut(s) 20, 1602
BsmFI GGGAC 3 cut(s) 898, 1189, 2651
BsmI GAATGC 4 cut(s) 638, 817, 1505, 2777
BsnI GGCC 5 cut(s) 992, 1541, 1814, 1917, 1963
BsoBI CYCGRG 2 cut(s) 1197, 1785
Bsp120I GGGCCC 1 cut(s) 1539
Bsp1286I GDGCHC 4 cut(s) 1198, 1543, 1786, 3527
Bsp13I TCCGGA 1 cut(s) 2716
Bsp1407I TGTACA 2 cut(s) 1057, 2430
Bsp19I CCATGG 1 cut(s) 1365
BspACI CCGC 7 cut(s) 56, 528, 1439, 1919, 1921, 3004, 3385
BspANI GGCC 5 cut(s) 992, 1541, 1814, 1917, 1963
BspCNI CTCAG 5 cut(s) 609, 2259, 2743, 3162, 3325
BspEI TCCGGA 1 cut(s) 2716
BspFNI CGCG 1 cut(s) 1921
BspLI GGNNCC 6 cut(s) 684, 1235, 1373, 1541, 2710, 3524
BspQI GCTCTTC 1 cut(s) 701
BspT107I GGYRCC 1 cut(s) 3522
BspTI CTTAAG 1 cut(s) 92
BsrBI CCGCTC 1 cut(s) 530
BsrDI GCAATG 3 cut(s) 74, 1044, 1899
BsrFI RCCGGY 1 cut(s) 334
BsrGI TGTACA 2 cut(s) 1057, 2430
BsrI ACTGG 4 cut(s) 153, 237, 2726, 3024
BssAI RCCGGY 1 cut(s) 334
BssECI CCNNGG 7 cut(s) 916, 1365, 1919, 2809, 2953, 3283, 3661
BssNI GRCGYC 5 cut(s) 240, 291, 342, 348, 700
BssT1I CCWWGG 1 cut(s) 1365
Bst2UI CCWGG 3 cut(s) 2954, 2994, 3284
Bst4CI ACNGT 9 cut(s) 1844, 2430, 2444, 2810, 3057, 3272, 3394, 3412, 3657
Bst6I CTCTTC 8 cut(s) 167, 358, 598, 701, 861, 2928, 3299, 3603
BstACI GRCGYC 5 cut(s) 240, 291, 342, 348, 700
BstAFI CTTAAG 1 cut(s) 92
BstAPI GCANNNNNTGC 2 cut(s) 87, 2888
BstAUI TGTACA 2 cut(s) 1057, 2430
BstBAI YACGTR 2 cut(s) 384, 3233
BstC8I GCNNGC 5 cut(s) 88, 1832, 1919, 1997, 3579
BstDEI CTNAG 9 cut(s) 617, 732, 2114, 2267, 2306, 2751, 2929, 3170, 3312
BstDSI CCRYGG 3 cut(s) 1365, 1919, 2809
BstEII GGTNACC 1 cut(s) 2957
BstFNI CGCG 1 cut(s) 1921
BstH2I RGCGCY 2 cut(s) 977, 1423
BstHHI GCGC 3 cut(s) 976, 1422, 1505
BstMAI GTCTC 6 cut(s) 20, 613, 639, 1602, 3104, 3646
BstMCI CGRYCG 1 cut(s) 890
BstMWI GCNNNNNNNGC 6 cut(s) 87, 806, 1020, 1097, 1526, 2888
BstNI CCWGG 3 cut(s) 2954, 2994, 3284
BstNSI RCATGY 4 cut(s) 1052, 1317, 2437, 2901
BstPI GGTNACC 1 cut(s) 2957
BstSCI CCNGG 5 cut(s) 809, 1229, 2952, 2992, 3282
BstSLI GKGCMC 2 cut(s) 1543, 3527
BstUI CGCG 1 cut(s) 1921
BstV1I GCAGC 5 cut(s) 781, 950, 1087, 1564, 1688
BstV2I GAAGAC 2 cut(s) 3339, 3413
BstX2I RGATCY 8 cut(s) 307, 499, 682, 1233, 2064, 2110, 2996, 3190
BstXI CCANNNNNNTGG 1 cut(s) 2011
BstYI RGATCY 8 cut(s) 307, 499, 682, 1233, 2064, 2110, 2996, 3190
BsuI GTATCC 2 cut(s) 1117, 3355
BsuRI GGCC 5 cut(s) 992, 1541, 1814, 1917, 1963
BtgI CCRYGG 3 cut(s) 1365, 1919, 2809
BtsI GCAGTG 2 cut(s) 779, 3349
BtsIMutI CAGTG 7 cut(s) 779, 1809, 2329, 2691, 3320, 3349, 3399
Cac8I GCNNGC 5 cut(s) 88, 1832, 1919, 1997, 3579
CaiI CAGNNNCTG 1 cut(s) 232
CfoI GCGC 3 cut(s) 976, 1422, 1505
Cfr10I RCCGGY 1 cut(s) 334
Cfr13I GGNCC 6 cut(s) 1539, 1540, 1813, 1916, 2587, 3221
Cfr42I CCGCGG 1 cut(s) 1922
CseI GACGC 3 cut(s) 356, 1584, 3206
Csp6I GTAC 4 cut(s) 1058, 2431, 3053, 3460
CspAI ACCGGT 1 cut(s) 334
CspCI CAANNNNNGTGG 4 cut(s) 982, 1017, 3102, 3137
CviQI GTAC 4 cut(s) 1058, 2431, 3053, 3460
DdeI CTNAG 9 cut(s) 617, 732, 2114, 2267, 2306, 2751, 2929, 3170, 3312
DraI TTTAAA 1 cut(s) 832
DraIII CACNNNGTG 1 cut(s) 603
DrdI GACNNNNNNGTC 1 cut(s) 2726
DseDI GACNNNNNNGTC 1 cut(s) 2726
EaeI YGGCCR 1 cut(s) 1961
Eam1104I CTCTTC 8 cut(s) 167, 358, 598, 701, 861, 2928, 3299, 3603
EarI CTCTTC 8 cut(s) 167, 358, 598, 701, 861, 2928, 3299, 3603
Eco130I CCWWGG 1 cut(s) 1365
Eco24I GRGCYC 3 cut(s) 1198, 1543, 1786
Eco32I GATATC 3 cut(s) 213, 1726, 2403
Eco47I GGWCC 2 cut(s) 2587, 3221
Eco57I CTGAAG 6 cut(s) 394, 1635, 1932, 2984, 3272, 3623
Eco72I CACGTG 1 cut(s) 3233
Eco88I CYCGRG 2 cut(s) 1197, 1785
Eco91I GGTNACC 1 cut(s) 2957
EcoO109I RGGNCCY 3 cut(s) 1539, 1540, 1813
EcoO65I GGTNACC 1 cut(s) 2957
EcoRI GAATTC 3 cut(s) 657, 1166, 1287
EcoRII CCWGG 3 cut(s) 2952, 2992, 3282
EcoRV GATATC 3 cut(s) 213, 1726, 2403
EcoT14I CCWWGG 1 cut(s) 1365
EcoT22I ATGCAT 3 cut(s) 638, 1643, 1872
EcoT38I GRGCYC 3 cut(s) 1198, 1543, 1786
ErhI CCWWGG 1 cut(s) 1365
Esp3I CGTCTC 2 cut(s) 20, 1602
FalI AAGNNNNNCTT 2 cut(s) 270, 302
FaqI GGGAC 3 cut(s) 898, 1189, 2651
FauI CCCGC 3 cut(s) 1432, 1926, 3378
FauNDI CATATG 1 cut(s) 3212
FbaI TGATCA 1 cut(s) 1932
FblI GTMKAC 3 cut(s) 345, 1008, 1510
Fnu4HI GCNGC 5 cut(s) 770, 939, 1101, 1578, 1702
FriOI GRGCYC 3 cut(s) 1198, 1543, 1786
Fsp4HI GCNGC 5 cut(s) 770, 939, 1101, 1578, 1702
FspBI CTAG 5 cut(s) 747, 1827, 2358, 2945, 3194
GlaI GCGC 3 cut(s) 975, 1421, 1504
GluI GCNGC 5 cut(s) 770, 939, 1101, 1578, 1702
GsaI CCCAGC 2 cut(s) 1160, 2739
GsuI CTGGAG 2 cut(s) 2012, 3041
HaeII RGCGCY 2 cut(s) 977, 1423
HaeIII GGCC 5 cut(s) 992, 1541, 1814, 1917, 1963
HapII CCGG 4 cut(s) 335, 810, 1230, 2717
HgaI GACGC 3 cut(s) 356, 1584, 3206
HhaI GCGC 3 cut(s) 976, 1422, 1505
Hin1I GRCGYC 5 cut(s) 240, 291, 342, 348, 700
Hin6I GCGC 3 cut(s) 974, 1420, 1503
HinP1I GCGC 3 cut(s) 974, 1420, 1503
HincII GTYRAC 4 cut(s) 238, 340, 346, 2731
HindII GTYRAC 4 cut(s) 238, 340, 346, 2731
HindIII AAGCTT 2 cut(s) 1076, 2234
HpaII CCGG 4 cut(s) 335, 810, 1230, 2717
Hpy166II GTNNAC 7 cut(s) 238, 340, 346, 1009, 1058, 1511, 2731
Hpy8I GTNNAC 7 cut(s) 238, 340, 346, 1009, 1058, 1511, 2731
Hpy99I CGWCG 5 cut(s) 245, 296, 347, 350, 1183
HpyCH4III ACNGT 9 cut(s) 1844, 2430, 2444, 2810, 3057, 3272, 3394, 3412, 3657
HpyCH4IV ACGT 9 cut(s) 144, 240, 291, 342, 383, 700, 2578, 2761, 3232
HpyF10VI GCNNNNNNNGC 6 cut(s) 87, 806, 1020, 1097, 1526, 2888
HpyF3I CTNAG 9 cut(s) 617, 732, 2114, 2267, 2306, 2751, 2929, 3170, 3312
HpySE526I ACGT 9 cut(s) 144, 240, 291, 342, 383, 700, 2578, 2761, 3232
Hsp92I GRCGYC 5 cut(s) 240, 291, 342, 348, 700
HspAI GCGC 3 cut(s) 974, 1420, 1503
Kpn2I TCCGGA 1 cut(s) 2716
Ksp22I TGATCA 1 cut(s) 1932
KspI CCGCGG 1 cut(s) 1922
LguI GCTCTTC 1 cut(s) 701
LmnI GCTCC 3 cut(s) 382, 1371, 3232
Lsp1109I GCAGC 5 cut(s) 781, 950, 1087, 1564, 1688
LweI GCATC 8 cut(s) 444, 712, 772, 1857, 2301, 2548, 2577, 2869
MaeI CTAG 5 cut(s) 747, 1827, 2358, 2945, 3194
MaeII ACGT 9 cut(s) 144, 240, 291, 342, 383, 700, 2578, 2761, 3232
MaeIII GTNAC 6 cut(s) 133, 185, 2786, 2817, 2957, 2985
MbiI CCGCTC 1 cut(s) 530
MfeI CAATTG 5 cut(s) 564, 1731, 2294, 3114, 3326
MflI RGATCY 8 cut(s) 307, 499, 682, 1233, 2064, 2110, 2996, 3190
MhlI GDGCHC 4 cut(s) 1198, 1543, 1786, 3527
MlsI TGGCCA 1 cut(s) 1963
MluNI TGGCCA 1 cut(s) 1963
MlyI GAGTC 3 cut(s) 1014, 2112, 2689
MmeI TCCRAC 2 cut(s) 1006, 2614
Mox20I TGGCCA 1 cut(s) 1963
Mph1103I ATGCAT 3 cut(s) 638, 1643, 1872
MroI TCCGGA 1 cut(s) 2716
MroXI GAANNNNTTC 2 cut(s) 113, 1798
MscI TGGCCA 1 cut(s) 1963
MslI CAYNNNNRTG 6 cut(s) 122, 627, 1053, 2795, 3354, 3541
Msp20I TGGCCA 1 cut(s) 1963
MspA1I CMGCKG 2 cut(s) 365, 1921
MspCI CTTAAG 1 cut(s) 92
MspI CCGG 4 cut(s) 335, 810, 1230, 2717
MspR9I CCNGG 5 cut(s) 811, 1231, 2954, 2994, 3284
MunI CAATTG 5 cut(s) 564, 1731, 2294, 3114, 3326
Mva1269I GAATGC 4 cut(s) 638, 817, 1505, 2777
MvaI CCWGG 3 cut(s) 2954, 2994, 3284
MvnI CGCG 1 cut(s) 1921
MwoI GCNNNNNNNGC 6 cut(s) 87, 806, 1020, 1097, 1526, 2888
NciI CCSGG 2 cut(s) 811, 1231
NcoI CCATGG 1 cut(s) 1365
NdeI CATATG 1 cut(s) 3212
NlaIV GGNNCC 6 cut(s) 684, 1235, 1373, 1541, 2710, 3524
NmeAIII GCCGAG 1 cut(s) 3686
NmuCI GTSAC 2 cut(s) 133, 2957
NsiI ATGCAT 3 cut(s) 638, 1643, 1872
NspI RCATGY 4 cut(s) 1052, 1317, 2437, 2901
OliI CACNNNNGTG 2 cut(s) 627, 3354
PciI ACATGT 2 cut(s) 1048, 2897
PciSI GCTCTTC 1 cut(s) 701
PctI GAATGC 4 cut(s) 638, 817, 1505, 2777
PdmI GAANNNNTTC 2 cut(s) 113, 1798
PfeI GAWTC 9 cut(s) 368, 474, 551, 1412, 1676, 1823, 2242, 3607, 3613
PflMI CCANNNNNTGG 1 cut(s) 1366
PinAI ACCGGT 1 cut(s) 334
PkrI GCNGC 5 cut(s) 771, 940, 1102, 1579, 1703
PleI GAGTC 3 cut(s) 1013, 2111, 2688
PmaCI CACGTG 1 cut(s) 3233
PmlI CACGTG 1 cut(s) 3233
PpsI GAGTC 3 cut(s) 1013, 2111, 2688
Ppu21I YACGTR 2 cut(s) 384, 3233
PscI ACATGT 2 cut(s) 1048, 2897
PsiI TTATAA 1 cut(s) 675
Psp6I CCWGG 3 cut(s) 2952, 2992, 3282
PspCI CACGTG 1 cut(s) 3233
PspEI GGTNACC 1 cut(s) 2957
PspFI CCCAGC 2 cut(s) 1156, 2735
PspGI CCWGG 3 cut(s) 2952, 2992, 3282
PspN4I GGNNCC 6 cut(s) 684, 1235, 1373, 1541, 2710, 3524
PspOMI GGGCCC 1 cut(s) 1539
PspPI GGNCC 6 cut(s) 1539, 1540, 1813, 1916, 2587, 3221
PstNI CAGNNNCTG 1 cut(s) 232
PsuI RGATCY 8 cut(s) 307, 499, 682, 1233, 2064, 2110, 2996, 3190
PvuII CAGCTG 1 cut(s) 365
RsaI GTAC 4 cut(s) 1059, 2432, 3054, 3461
RsaNI GTAC 4 cut(s) 1058, 2431, 3053, 3460
RseI CAYNNNNRTG 6 cut(s) 122, 627, 1053, 2795, 3354, 3541
SacII CCGCGG 1 cut(s) 1922
SalI GTCGAC 1 cut(s) 344
SapI GCTCTTC 1 cut(s) 701
SatI GCNGC 5 cut(s) 770, 939, 1101, 1578, 1702
Sau96I GGNCC 6 cut(s) 1539, 1540, 1813, 1916, 2587, 3221
SchI GAGTC 3 cut(s) 1014, 2112, 2689
ScrFI CCNGG 5 cut(s) 811, 1231, 2954, 2994, 3284
SduI GDGCHC 4 cut(s) 1198, 1543, 1786, 3527
SfaNI GCATC 8 cut(s) 444, 712, 772, 1857, 2301, 2548, 2577, 2869
Sfr303I CCGCGG 1 cut(s) 1922
SgrBI CCGCGG 1 cut(s) 1922
SgrDI CGTCGACG 1 cut(s) 344
SinI GGWCC 2 cut(s) 2587, 3221
SmiMI CAYNNNNRTG 6 cut(s) 122, 627, 1053, 2795, 3354, 3541
SmlI CTYRAG 3 cut(s) 92, 2237, 2677
SmoI CTYRAG 3 cut(s) 92, 2237, 2677
SsiI CCGC 7 cut(s) 56, 528, 1439, 1919, 1921, 3004, 3385
SspI AATATT 2 cut(s) 2075, 2497
SspMI CTAG 5 cut(s) 747, 1827, 2358, 2945, 3194
StyD4I CCNGG 5 cut(s) 809, 1229, 2952, 2992, 3282
StyI CCWWGG 1 cut(s) 1365
TaaI ACNGT 9 cut(s) 1844, 2430, 2444, 2810, 3057, 3272, 3394, 3412, 3657
TaiI ACGT 9 cut(s) 147, 243, 294, 345, 386, 703, 2581, 2764, 3235
TaqI TCGA 5 cut(s) 345, 457, 1546, 1582, 2170
TaqII GACCGA 1 cut(s) 904
TatI WGTACW 3 cut(s) 1057, 2430, 3052
TfiI GAWTC 9 cut(s) 368, 474, 551, 1412, 1676, 1823, 2242, 3607, 3613
TscAI CASTG 7 cut(s) 779, 1809, 2329, 2698, 3320, 3356, 3399
TseFI GTSAC 2 cut(s) 133, 2957
TseI GCWGC 5 cut(s) 769, 938, 1100, 1577, 1701
Tsp45I GTSAC 2 cut(s) 133, 2957
TspGWI ACGGA 3 cut(s) 771, 1196, 1869
TspRI CASTG 7 cut(s) 779, 1809, 2329, 2698, 3320, 3356, 3399
Van91I CCANNNNNTGG 1 cut(s) 1366
Vha464I CTTAAG 1 cut(s) 92
VpaK11BI GGWCC 2 cut(s) 2587, 3221
XbaI TCTAGA 3 cut(s) 2357, 2944, 3193
XceI RCATGY 4 cut(s) 1052, 1317, 2437, 2901
XcmI CCANNNNNNNNNTGG 2 cut(s) 381, 604
XmiI GTMKAC 3 cut(s) 345, 1008, 1510
XmnI GAANNNNTTC 2 cut(s) 113, 1798
XspI CTAG 5 cut(s) 747, 1827, 2358, 2945, 3194
ZraI GACGTC 4 cut(s) 241, 292, 343, 701
Zsp2I ATGCAT 3 cut(s) 638, 1643, 1872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.