Rw5G048540

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000085
Physical Location & Seq
Reverse (-)
84390523 .. 84400494
9972 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G048540.1

Sequence Viewer

Length: 6855 bp
ATGGCTTCTCTTCCTTCATCAGCTGAACCATCATCAGCAGCTCGTCGCTGGAAGTATGACGTGTATTTGAGTTTCAGGGGTGCTGACACTCGCAGGGGTTTTACATCGCAACTATACGATGGGCTGCAGAGGAGGGGAGTTAAAACATTCATGGATCATCATGATCTTCAAGTAGGGGATGATATTGATGCCACTGTCCTAAAGGCAATTGAAGAATCAAGGTTCACAATTGTTGTTCTGTCCCAAAATTATGCATATTCTACTTGGTGTTTGGAGGAACTTACAAAGATTTGGCAGTTCATGGAGGACGGCCCCAGAATTCTTCCAGTTTTTTATGATGTCGAGCCTAGTGATGTAAGACATCAAAAGGGGAGTTTTCAGGATGCTTTCACCAAGCATGAAAGGTCTGGGCGATACGAACCGGAGAAGGTCCAGCTGTGGAGAGATGCTTTAAGAAGAACGACCAATTTAGCTGTGTGGGATACAAGGAGGTACAGCACTGAGACAGGAGTTATCGAAGCCATTGTGAAATTTGTGTACAGAAAACTGCGGTCTGTTGCGGGAATCCACACTGGAGATTTTGAAGCTGCAAGGCAAGCCACTATTGAAATTGAACCTACAATCTCTCGAAAAACTGCTGCATCTCTTCCTTCATCAGCTGAACCATCATCAACAGCTGGTCTGTGGAAGTATGACGTGTATGTGAGTTTTAGTGGTGTAGATACTCGAAGGGGTTTTACATCCCATTTATACGATCGGTTGCAAAAGAGAGGAATCACAACATTCATTGATTACCAAGATCTTAAACGAGGGGAGGCTATTTCTTCTCTCCTGGAGGCAATTGAAGGATCAAGGTTTGCAATTGTTGTTTTGTCCCAAAGCTATGCTTCTTCTCGTTGGTGTTTGGAGGAACTTACAAAGATTTGTCAGTTCATGGAGGACGGTCACAGAATTCTTCCAGTTTTTTATGATGTCGACCCTTGTGATCTAAGACATCAAACGGGGACTTTTCAAGATGCTTTCACCAAGCATGAAAAGTATGGGCGATGCGAACCGGAGGAGGTCCAGCAGTGGAGATATGCTTTAAGAAGAATGGGCAAGTTGTTTGGGTGGGTTGCTCAGGATTATAAGTATGAGACAGAATTTGTCAAAGCCGTTGTGGAATCTGTGTACAGTAGAGTACGATCCGTTTTGGAAATTGAAAAAGTACAACCTATTGAAATCAATACCGTTGTGGAATCTGTGTACAGTAGAGTACAATCCGTTTTGGAAATTGAAAAAGTACAACCTATTGAAATCAATACAGGAGGTTTTGAAGTCTTTGAAGCAACAAGAAAAGCCATGGATAAGGTTATGAAGGCGCTAAAAGATGACGACGTCACTGCTGTTGGGGTCTATGGCATGGGAGGCGTCGGCAAGACAACCATGGTAAAACATGTCGCTGCACATGCCCAAAAAAAAGGGATTTTTGATCATGTGATTATGGTTGTTGTTACCCAAAGCCCTGACTTGAGAAAAATTCAAGGCACATTGAAAGATCAGTTGAGCGTCAAATTGGAGTTAAGTACAGAAACTGGAAGAGCTGTTTCATTGAAATTCATGATGATGAGAATAATGAGAAAAGGAAAGATCCTTATAATCCTGGACGACATTTGGGAGAGATTGGACTTGCCAGACATAGGAATACCAAGCTACAAGGAACTTCAAAAGTGCAATTCCAAAGTCCTACTCACCACAAGGATAAGGAATGTCTGTCGTGCCATGGAGTGCCAAGTAATTACTCCTCTCAATATCTTATCAGAAGAAGATTCTTGGACCTTGTTTGTGAAAACGGCAAGAATAGACTCTGAATCGAACTGTTTCTATGATGTAGTGAGGGAGGTGGTTAGTGAATGTGCTGGTCTACCGATTGCATTGATAACAGTTGCTTGGGCACTTGGAGGTAAAGCACTAGAGGAATGGACAAGAGCAGCTCGAGCACTAGAGAAGTCGCGATCTGCCAACCCTGACTGTTACGGAGTAGTATCCAAATGTATAAAATCAAGTTATGATTACTTGAAAGATGAAGATTCCAAGTCATGCTTCTTGCTTTGTTGCCTATTTCCAGAAGGCTATGACATCCCAGTAGAAGACTTGTTCAGGTATGCCATCGGGAAAGGATTGTTTCAAGATGCCAACACAATTGAAGAAGCCAGAGGAATAGCAGATTCAGTGGTCAAGTACCTGAAAGATTCTAGCTTGCTTTTGGATAGCGAGAAAAAAGGATGTGTAAAGATGCATGATCTCATCCGGGATACAGCCCTGAATATTGCAAAATCTGAAGATGGGCATCGGTTTTTGGTAAAAGCTGGCTGTGGTTTAAAGAGCTGGCCACGTCAAGGACACGAAGGCTACTCTGCAATCTCATTAATGAACAATAATATCCACAAGCTACCCGAAGAGCTGGTATGTCCAAAACTCCAGATTTTATTACTACAGCAGAATGCTGATTTAAATGAGATCCCAGAAAATTTTTTCCACAGTTCTAATGAATTAAGGGTCTTAGATCTTAGCCTCACCAGCATTACGTTACTTCCTCAATCAATCAGTCTCCTAATGAACCTCCAGGCTTTGTATCTAGATTTTTGCCAGAAACTGATTGACTTCTGCATACTTGGAAAACTAAAAAAGCTTGAGATTCTTAGCATGAGAGGAAGTGTTCTGGAAGAATTGGCAATAGAATTAAGGTGTCTGATCAATCTGAGGATGCTGGACATTACTGATGGAATTATTGGCAAAATTCCATCGAAAGTGATATCCCAGTTGCATAGATTAGAAGAACTGTACCTGCCATTTCAATTTGGGGATTGGGTCAGTAAGGTTGAAGGAGAAGGAGGAGAAACAGATATTGGCTTTGGTGAGGTAACTGGCTTGTCATGTCTAAATATTTTGAAGGTTTCCATATCTGCAAAATGCATCCCTGAAGAGCTTGAGTTTTATCCCAATTGGTTGTACTTTGATATATGTATTACTGGAGATACAAGCAGCAGAGACATTATGAGTGACATAAGTAATCAAAGCTCTCGATATAGTAATAAAGATTCAAGATCCTTGAGTCTTGACACAAGAATTAATGCCTTACCAGCTTGGTTTGTCGATGAGGTAACAGTCAAAACAGAGAAGCTACTGTATGTAAAGTGCAAAAATTTAAATAACATTCTTGTGGAATATCACCATGGAAGGTTACATATGCTGAAGCATCTCTCTGTTATTGGTCCCAGTGAGAGCTTGGAGCAGTTGATGAATGCTATAACATGTGTTCCAAATAAACCGGTGTTCGAGAACTTGGAAGAGTTGCATTTGGAGAGTCTGGCTTCCTTGAAAGAGTTATGTGTCGGAAATTTACCAGCTGGATCACTCTACAATCTGAAGTTATTGAAGGTCCATTATTGTTCTAACTTGGGGAATATACTTTTACCATCAAAATTGTTACAGAGATTGCCAAATCTGGAGAACGTAATCTGTAAGGATATGTCTAGACTGGAATATGTATTTGGATGTCAAGGGTTTGAGCCAGAGCAGTCAAAACTGAGAGAGATGACATTCTTCAATCTACCTGCAGTAAGAAACATATGTAAGGGTCCTGCTCCACGTGCAATGTTCCAGGCTCTTAAAAGTTTGTTCATTTATCGTTGCAGCTTTCTAGGATGTCTCTTCTCCTTTGATACTGCTCAATTTCTCTTTCAATTGGAGGACCTTTTTGTAGACAGTTGCCCTTTATTGGAAAGACTAATTGAAGTACGCAAGGAAAGAGTGAACAACAAGAAGATTTTTTACAACAAAATCATTCTTCCAAAATTGAAGAACTTGGGTTTAGAGAAACTTCCTATGTTGTACAGTGGAAGTGGTACTATTGATATCGAGTGTCCTTCCTTGAAACACTTGTATGTGAAGGAGTGCCCCCAGTTTACAGCCTCTGCTTCTGACTTCCAGACCAAGAACCAAGTCCTGGTCAACGATAAATTACATTACTTTGGTCTACTAGGCAGAAGGACTGAAAGACAACATATTGAAGCCATTGCGGAATCTGTGTTCAGTACAGTACGACCTATTGAACCTGAGTTTACAATGCCCATGGGAGATTTTCAAGCATTTGAAGCAACAAGACATGCCATGGATAAGGTTATGAAGGCACTAGAAGCTGATGAGGTCACAGCCATTGGGGTCTACGGAATGGGAGGAGTTGGAAAATCAACCATGGTTGAACATGTTTGTGCAGAAGCTCGGGATAATGGGATGTTTCATCAGGTGATTTCGGCTGTTGTATCCCAAAACCCTGACTTGAGAAAAATTCAAGGCACATTGGCAGATATGTTGGGCTTTATATTTGAGGAGGAGACAGAAATTGGAAGAGCTCGTAGATTGATGCAGAGGATAAGGAGAGGAAATAGGATCCTTATAATCTTGGACAACATTTGGGAGAGAACAGATTTGTCAAACATAGGAATTCCCAGCTACTACGAGCTTCAAAGATGCAATTCCAAAGTCCTCCTCATCACGAGGACACAGAATGTCTGTCGTGCCATGGAGTGTCAATCAAACATTTCTCTTGATGTCTTATCAGAAGAAGATTCTTGGACCTTATTTGTGAACAAAGCAAGAACATCTTTTGAATCGACCAATTTGTGTGATGTTGCAAAGAAGGTAGCTAGAGCATGTGCCGGTCTTCCAGTTGCGTTGATAGCAGTTGCGAAGGCACTTGGTGATAAGTGCTTGGATGACTGGAAGGAAGCAGCGCGACGACTAGAGGCTTCTCAACCTGCCAACCTTGAAGATGGGAAGGTTGTGTTCAATTGTATAAAATTAAGCTATGATTACTTGATATCTGGTGATGCTAGGTCATGCTTCTTGCTTTGTTGCCCATTCCCAGAAGACTATGATATCCAAATTGAAGAGTTGTTAAAGTATGGGATGGGGAAAGGATTGTTTCAAGATTCCTGCACAATGCAAGATGCCAGAGTAACTGCACATTTGGTGGTCAAGTACCTTAAAGATTGTAGCTTGCTTTTGGAAACTGAACAGGACGGATGTGTAAAGATGCATGATGTCATTCGGGATGTGGCCATAAGGATTTCGTCGTCTGAGGATGGCCCACTGTTTTTTGTACAAGCTGGTTGTCAATTAAAGGAATGGCCAAGTATTAGTGCACATGAAGGCTACTCTGCTATCTCACTAATTACGAATAAAATTTGCAAGCTTCCTGAAGAGTTGGTATGTCCGAAACTCCTTATTTTGTTTCTGCAAAGTAATGTTTGGTTGAATGAGATCCCAGAATATTTCTTTCAAAGTCTGAATGTGTTGAGGGTATTAGATCTAAGCCAAACTAGAATTTCTTTTCTACCCACATCATTCAATATCTTGATCAACCTCCAGACCTTGTATCTAGATGGTTGCTTGTCAACCATAGATAACATATCCATACTCGGAAAATTGAAAAAACTTGAGATTCTCAGTATGAGAGAACTTCCTCTTGTGGAACTGCCGAAAGAAATTGGAGATTTGAGCCATCTAAGGATGTTAGACATCACTGGTGATTATGTTGATAATGTTCCATCAGAAGTGATATGCAACCTTAATTCATTAGAGGAACTATACATGCAGTGTAATTTTGCCAACTGGGGGAGTAAAGTTGAGGGGGCAAGAGAAGAAAATATTGTTGCGTTTGCTGAGTTAACTGGCTTGTCATATCTGAACGTTTTGAAGGTTTTCATATCTGATGCTAAATGTTTGCCTAAAGATGTGAAGGTTGAGCCAAATTGGGTAAACTTTGATATTTGTATCAGCAGAGACCCGTTAATCAGAAAAGTATCCTTGGTTTCAAGTTCTTCACCCGCTTTTATAAGAGCCTTGACTCTTGACACAACCATCAATGCCTTACCGGGTTGGTTTATCAATGCAGTGACAATGAAAGCAGAGAAACTACAGTACATAATGTGTTGGGGATTAGATAACATTCTTGTGGAATTTGACTGTGGGAGATTACATGCACTGAAGTATTTATCCGTTATTGGGCCCATGGAGAACTTGAAAGTGTTGATGAACACAGTGACACGCTTTCGGAATGAACCAGTGTTTCAGAACTTGGAAGAGTTGCATCTCGTTCAAGTGGATTGCCTGAAGGAGTTATGTGTTGGTGAATTGCCGCCTGGGTCTCTATGCAATCTTAGGTTACTGAAGGTGAAACATTGCTATGAATTGGTGGATGCACTCCTACCGTCACGGTTGCTGCAGAGATTACAAAATCTGAAAGAACTGATTTGTGAAGAGATTGATGAATTGGAATATGTTTTTGGATGTGAAGGGCTTGAGCCAAAACAATTGATATTGACAGAATTGATAGAAATGAGATTGGAGAATCTAGGGAAACTAATAAAGATATGGAATGGTCCTGCTCATGGTGCAATCTTCCAGAATCTTCGGAGTTTGGTAGTTTCCCGGTGCTTTGAACTGGAAAATCTCTTCACAGCTGATGTAGCTCAATATTTTTTCCACTTGGAAGATCTTTCAGTGGAGCTTTGCCCAATGTTGGACAACATTATTGAAGCAAGTCAGGACACAGTGCACAACAAGATTGTTTTTCCAGAATTGAAGAACTTAACTTTGATAAAGCTTCCACAACTCACAAGGTTCTGCGGTAGTACTGGAAGTGCTACCGAGTGCCCTTTACTGGAATACTTGTATGTGGAGAGGTGCCCCCAGTTTTTAACGTCTGACTTCCATAGCAGGAACAAAGTCCAACTCAATCATCCACAACATTTGGACATTGTACAGAGAAGGGCCCAGAGCAGAGAAAAGAAGAAAAATCAATTTCACTGA

Protein Analysis

2284

Amino Acids

259.33

Weight (kDa)

5.84

Isoelectric Point (pI)

48.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 18 - 187 1.8e-44 TIR domain
TIR_2 PF13676 21 - 117 5.3e-18 TIR domain
TIR PF01582 230 - 399 4.2e-49 TIR domain
TIR_2 PF13676 233 - 330 2.4e-16 TIR domain
NB-ARC PF00931 446 - 612 1.5e-32 NB-ARC domain
LRR_8 PF13855 818 - 877 4e-06 Leucine rich repeat
LRR_14 PF23598 828 - 941 3.9e-06 Leucine-rich repeat region
LRR_RPS2 PF23247 1109 - 1251 6.3e-21 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1197 - 1317 7.1e-13 Plant disease resistance protein RPS2-like, leucine-rich repeats
NB-ARC PF00931 1384 - 1546 2.4e-28 NB-ARC domain
LRR_8 PF13855 1753 - 1811 3.4e-06 Leucine rich repeat
LRR_14 PF23598 1804 - 1881 3.3e-10 Leucine-rich repeat region
LRR_RPS2 PF23247 2041 - 2186 1.3e-26 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 1128, 1637, 4439, 5879
AatII GACGTC 1 cut(s) 1380
Acc36I ACCTGC 3 cut(s) 2835, 3604, 4807
AccB1I GGYRCC 1 cut(s) 6729
AccB7I CCANNNNNTGG 1 cut(s) 3988
AccI GTMKAC 5 cut(s) 975, 1903, 3744, 4018, 4206
AccII CGCG 2 cut(s) 1993, 4777
AciI CCGC 6 cut(s) 550, 560, 4061, 5871, 6182, 6672
AclI AACGTT 1 cut(s) 5733
AcoI YGGCCR 3 cut(s) 2369, 5100, 5171
AcuI CTGAAG 8 cut(s) 2340, 2982, 3254, 3428, 5262, 6050, 6176, 6233
AcvI CACGTG 1 cut(s) 3632
AcyI GRCGYC 2 cut(s) 1377, 1410
AdeI CACNNNGTG 1 cut(s) 4742
AfiI CCNNNNNNNGG 9 cut(s) 1679, 2378, 3760, 3988, 5658, 6048, 6434, 6565, 6706
AflIII ACRYGT 5 cut(s) 60, 696, 1435, 3293, 4246
AgeI ACCGGT 1 cut(s) 3310
AjiI CACGTC 3 cut(s) 61, 697, 2375
AjnI CCWGG 6 cut(s) 831, 1641, 2604, 3642, 3987, 6184
AjuI GAANNNNNNNTTGG 8 cut(s) 2066, 2098, 2871, 2903, 3516, 3548, 6312, 6344
AloI GAACNNNNNNTCC 2 cut(s) 4055, 4087
Alw21I GWGCWC 4 cut(s) 1981, 4396, 5188, 6603
Alw26I GTCTC 8 cut(s) 497, 1130, 2594, 3024, 3695, 4370, 5820, 6195
Alw44I GTGCAC 2 cut(s) 5184, 6599
AlwNI CAGNNNCTG 3 cut(s) 1574, 2635, 3956
Ama87I CYCGRG 2 cut(s) 1974, 4263
AoxI GGCC 7 cut(s) 310, 2369, 5100, 5128, 5171, 6050, 6816
ApaI GGGCCC 2 cut(s) 6054, 6820
ApaLI GTGCAC 2 cut(s) 5184, 6599
AseI ATTAAT 2 cut(s) 2408, 3111
AsiGI ACCGGT 1 cut(s) 3310
Asp700I GAANNNNTTC 2 cut(s) 1859, 5744
AspLEI GCGC 2 cut(s) 1363, 4777
AsuC2I CCSGG 3 cut(s) 2291, 5919, 6475
AvaI CYCGRG 2 cut(s) 1974, 4263
AvaII GGWCC 9 cut(s) 430, 1063, 1815, 3254, 3421, 3620, 3733, 4617, 6425
BaeGI GKGCMC 8 cut(s) 1936, 3941, 5188, 6054, 6603, 6701, 6734, 6820
BalI TGGCCA 3 cut(s) 2371, 5102, 5173
BamHI GGATCC 1 cut(s) 4431
BanI GGYRCC 1 cut(s) 6729
BanII GRGCYC 3 cut(s) 4396, 6054, 6820
BarI GAAGNNNNNNTAC 4 cut(s) 721, 753, 3797, 3829
BauI CACGAG 1 cut(s) 4537
BbrPI CACGTG 1 cut(s) 3632
BbsI GAAGAC 3 cut(s) 2136, 4697, 4917
Bbv12I GWGCWC 4 cut(s) 1981, 4396, 5188, 6603
BceAI ACGGC 3 cut(s) 325, 1139, 1848
BcgI CGANNNNNNTGC 2 cut(s) 1364, 1398
BciT130I CCWGG 6 cut(s) 833, 1643, 2606, 3644, 3989, 6186
BciVI GTATCC 5 cut(s) 475, 2035, 2287, 4315, 5857
BclI TGATCA 3 cut(s) 1471, 2733, 5400
BcnI CCSGG 3 cut(s) 2291, 5919, 6475
BcoDI GTCTC 8 cut(s) 497, 1130, 2594, 3024, 3695, 4370, 5820, 6195
BfmI CTRYAG 5 cut(s) 125, 2474, 3597, 5960, 6266
BfoI RGCGCY 1 cut(s) 1364
BfuAI ACCTGC 3 cut(s) 2835, 3604, 4807
BfuI GTATCC 5 cut(s) 475, 2035, 2287, 4315, 5857
BglII AGATCT 4 cut(s) 799, 2545, 5350, 6538
BmcAI AGTACT 1 cut(s) 6679
Bme1390I CCNGG 9 cut(s) 833, 1643, 2291, 2606, 3644, 3989, 5919, 6186, 6475
Bme18I GGWCC 9 cut(s) 430, 1063, 1815, 3254, 3421, 3620, 3733, 4617, 6425
BmeT110I CYCGRG 2 cut(s) 1974, 4263
BmgBI CACGTC 3 cut(s) 61, 697, 2375
BmiI GGNNCC 7 cut(s) 313, 3256, 3621, 4433, 6052, 6731, 6818
BmrFI CCNGG 9 cut(s) 833, 1643, 2291, 2606, 3644, 3989, 5919, 6186, 6475
BmrI ACTGGG 6 cut(s) 2117, 2794, 3252, 3937, 5665, 6730
BmuI ACTGGG 6 cut(s) 2117, 2794, 3252, 3937, 5665, 6730
BpiI GAAGAC 3 cut(s) 2136, 4697, 4917
BpmI CTGGAG 7 cut(s) 594, 854, 2444, 2588, 3033, 3509, 5393
Bpu10I CCTNAGC 1 cut(s) 1119
BpuEI CTTGAG 7 cut(s) 1531, 2693, 2990, 3112, 4342, 5501, 6365
BpuMI CCSGG 3 cut(s) 2291, 5919, 6475
BsaAI YACGTR 1 cut(s) 3632
BsaBI GATNNNNATC 1 cut(s) 2328
BsaHI GRCGYC 2 cut(s) 1377, 1410
BsaI GGTCTC 2 cut(s) 5820, 6195
BsaWI WCCGGW 3 cut(s) 421, 1054, 3310
Bsc4I CCNNNNNNNGG 9 cut(s) 1679, 2378, 3760, 3988, 5658, 6048, 6434, 6565, 6706
Bse118I RCCGGY 2 cut(s) 3310, 4700
Bse3DI GCAATG 3 cut(s) 3642, 4056, 6223
Bse8I GATNNNNATC 1 cut(s) 2328
BseBI CCWGG 6 cut(s) 833, 1643, 2606, 3644, 3989, 6186
BseJI GATNNNNATC 1 cut(s) 2328
BseLI CCNNNNNNNGG 9 cut(s) 1679, 2378, 3760, 3988, 5658, 6048, 6434, 6565, 6706
BseMI GCAATG 3 cut(s) 3642, 4056, 6223
BseMII CTCAG 8 cut(s) 492, 1133, 2732, 3560, 4089, 5112, 5503, 5697
BseRI GAGGAG 8 cut(s) 145, 1073, 1773, 2889, 4233, 4385, 4388, 4520
BseSI GKGCMC 8 cut(s) 1936, 3941, 5188, 6054, 6603, 6701, 6734, 6820
BseYI CCCAGC 1 cut(s) 4490
BsgI GTGCAG 4 cut(s) 1428, 4275, 4963, 4989
Bsh1236I CGCG 2 cut(s) 1993, 4777
Bsh1285I CGRYCG 1 cut(s) 757
BshFI GGCC 7 cut(s) 312, 2371, 5102, 5130, 5173, 6052, 6818
BshNI GGYRCC 1 cut(s) 6729
BshTI ACCGGT 1 cut(s) 3310
BsiEI CGRYCG 1 cut(s) 757
BsiHKAI GWGCWC 4 cut(s) 1981, 4396, 5188, 6603
BsiHKCI CYCGRG 2 cut(s) 1974, 4263
BsiSI CCGG 7 cut(s) 422, 1055, 2290, 3311, 4701, 5918, 6475
BslFI GGGAC 4 cut(s) 226, 859, 1018, 3240
BslI CCNNNNNNNGG 9 cut(s) 1679, 2378, 3760, 3988, 5658, 6048, 6434, 6565, 6706
BsmAI GTCTC 8 cut(s) 497, 1130, 2594, 3024, 3695, 4370, 5820, 6195
BsmFI GGGAC 4 cut(s) 226, 859, 1018, 3240
BsmI GAATGC 2 cut(s) 2488, 3289
BsnI GGCC 7 cut(s) 312, 2371, 5102, 5130, 5173, 6052, 6818
Bso31I GGTCTC 2 cut(s) 5820, 6195
BsoBI CYCGRG 2 cut(s) 1974, 4263
Bsp120I GGGCCC 2 cut(s) 6050, 6816
Bsp1407I TGTACA 6 cut(s) 537, 1170, 1245, 3873, 5143, 6805
Bsp19I CCATGG 9 cut(s) 1341, 1425, 1761, 3214, 4113, 4152, 4236, 4563, 6054
Bsp68I TCGCGA 1 cut(s) 1993
BspACI CCGC 6 cut(s) 550, 560, 4061, 5871, 6182, 6672
BspANI GGCC 7 cut(s) 312, 2371, 5102, 5130, 5173, 6052, 6818
BspCNI CTCAG 8 cut(s) 493, 1132, 2733, 3561, 4090, 5113, 5502, 5698
BspFNI CGCG 2 cut(s) 1993, 4777
BspHI TCATGA 2 cut(s) 160, 1599
BspLI GGNNCC 7 cut(s) 313, 3256, 3621, 4433, 6052, 6731, 6818
BspMAI CTGCAG 3 cut(s) 129, 3601, 6270
BspMI ACCTGC 3 cut(s) 2835, 3604, 4807
BspQI GCTCTTC 4 cut(s) 1573, 2433, 2958, 4384
BspT107I GGYRCC 1 cut(s) 6729
BspTNI GGTCTC 2 cut(s) 5820, 6195
BsrDI GCAATG 3 cut(s) 3642, 4056, 6223
BsrFI RCCGGY 2 cut(s) 3310, 4700
BsrGI TGTACA 6 cut(s) 537, 1170, 1245, 3873, 5143, 6805
BssAI RCCGGY 2 cut(s) 3310, 4700
BssNI GRCGYC 2 cut(s) 1377, 1410
BssSI CACGAG 1 cut(s) 4537
Bst2BI CACGAG 1 cut(s) 4537
Bst2UI CCWGG 6 cut(s) 833, 1643, 2606, 3644, 3989, 6186
BstACI GRCGYC 2 cut(s) 1377, 1410
BstAUI TGTACA 6 cut(s) 537, 1170, 1245, 3873, 5143, 6805
BstBAI YACGTR 1 cut(s) 3632
BstC8I GCNNGC 6 cut(s) 597, 2240, 2350, 2369, 5042, 5234
BstDSI CCRYGG 9 cut(s) 1341, 1425, 1761, 3214, 4113, 4152, 4236, 4563, 6054
BstFNI CGCG 2 cut(s) 1993, 4777
BstH2I RGCGCY 1 cut(s) 1364
BstHHI GCGC 2 cut(s) 1363, 4777
BstMAI GTCTC 8 cut(s) 497, 1130, 2594, 3024, 3695, 4370, 5820, 6195
BstMCI CGRYCG 1 cut(s) 757
BstNI CCWGG 6 cut(s) 833, 1643, 2606, 3644, 3989, 6186
BstNSI RCATGY 8 cut(s) 1439, 1451, 3297, 4151, 4250, 4698, 5638, 6026
BstSCI CCNGG 9 cut(s) 831, 1641, 2289, 2604, 3642, 3987, 5917, 6184, 6473
BstSFI CTRYAG 5 cut(s) 125, 2474, 3597, 5960, 6266
BstSLI GKGCMC 8 cut(s) 1936, 3941, 5188, 6054, 6603, 6701, 6734, 6820
BstUI CGCG 2 cut(s) 1993, 4777
BstV2I GAAGAC 3 cut(s) 2136, 4697, 4917
BstX2I RGATCY 9 cut(s) 799, 1629, 2499, 2545, 3086, 4431, 5304, 5350, 6538
BstYI RGATCY 9 cut(s) 799, 1629, 2499, 2545, 3086, 4431, 5304, 5350, 6538
BsuI GTATCC 5 cut(s) 475, 2035, 2287, 4315, 5857
BsuRI GGCC 7 cut(s) 312, 2371, 5102, 5130, 5173, 6052, 6818
BtgI CCRYGG 9 cut(s) 1341, 1425, 1761, 3214, 4113, 4152, 4236, 4563, 6054
BtgZI GCGATG 2 cut(s) 90, 1060
BtrI CACGTC 3 cut(s) 61, 697, 2375
BtsI GCAGTG 4 cut(s) 1076, 1380, 5645, 5943
BtuMI TCGCGA 1 cut(s) 1993
BveI ACCTGC 3 cut(s) 2835, 3604, 4807
Cac8I GCNNGC 6 cut(s) 597, 2240, 2350, 2369, 5042, 5234
CaiI CAGNNNCTG 3 cut(s) 1574, 2635, 3956
CciI TCATGA 2 cut(s) 160, 1599
CfoI GCGC 2 cut(s) 1363, 4777
Cfr10I RCCGGY 2 cut(s) 3310, 4700
CseI GACGC 2 cut(s) 1399, 1537
CspAI ACCGGT 1 cut(s) 3310
CspCI CAANNNNNGTGG 2 cut(s) 6777, 6812
DraI TTTAAA 3 cut(s) 2361, 2493, 3189
DraIII CACNNNGTG 1 cut(s) 4742
EaeI YGGCCR 3 cut(s) 2369, 5100, 5171
Ecl136II GAGCTC 1 cut(s) 4394
Eco24I GRGCYC 3 cut(s) 4396, 6054, 6820
Eco31I GGTCTC 2 cut(s) 5820, 6195
Eco32I GATATC 4 cut(s) 2796, 3898, 4863, 4921
Eco47I GGWCC 9 cut(s) 430, 1063, 1815, 3254, 3421, 3620, 3733, 4617, 6425
Eco53kI GAGCTC 1 cut(s) 4394
Eco57I CTGAAG 8 cut(s) 2340, 2982, 3254, 3428, 5262, 6050, 6176, 6233
Eco72I CACGTG 1 cut(s) 3632
Eco88I CYCGRG 2 cut(s) 1974, 4263
EcoICRI GAGCTC 1 cut(s) 4394
EcoO109I RGGNCCY 3 cut(s) 3620, 3733, 6816
EcoRI GAATTC 3 cut(s) 318, 951, 4485
EcoRII CCWGG 6 cut(s) 831, 1641, 2604, 3642, 3987, 6184
EcoRV GATATC 4 cut(s) 2796, 3898, 4863, 4921
EcoT22I ATGCAT 4 cut(s) 256, 2280, 2957, 5082
EcoT38I GRGCYC 3 cut(s) 4396, 6054, 6820
FalI AAGNNNNNCTT 6 cut(s) 871, 903, 2066, 2098, 4630, 4662
FaqI GGGAC 4 cut(s) 226, 859, 1018, 3240
FauI CCCGC 2 cut(s) 553, 5878
FauNDI CATATG 2 cut(s) 3228, 3611
FbaI TGATCA 3 cut(s) 1471, 2733, 5400
FblI GTMKAC 5 cut(s) 975, 1903, 3744, 4018, 4206
FriOI GRGCYC 3 cut(s) 4396, 6054, 6820
GlaI GCGC 2 cut(s) 1362, 4776
GsaI CCCAGC 1 cut(s) 4494
GsuI CTGGAG 7 cut(s) 594, 854, 2444, 2588, 3033, 3509, 5393
HaeII RGCGCY 1 cut(s) 1364
HaeIII GGCC 7 cut(s) 312, 2371, 5102, 5130, 5173, 6052, 6818
HapII CCGG 7 cut(s) 422, 1055, 2290, 3311, 4701, 5918, 6475
HgaI GACGC 2 cut(s) 1399, 1537
HhaI GCGC 2 cut(s) 1363, 4777
Hin1I GRCGYC 2 cut(s) 1377, 1410
Hin6I GCGC 2 cut(s) 1361, 4775
HinP1I GCGC 2 cut(s) 1361, 4775
HincII GTYRAC 4 cut(s) 976, 3994, 5439, 5712
HindII GTYRAC 4 cut(s) 976, 3994, 5439, 5712
HindIII AAGCTT 3 cut(s) 2669, 5234, 6647
HpaI GTTAAC 1 cut(s) 5712
HpaII CCGG 7 cut(s) 422, 1055, 2290, 3311, 4701, 5918, 6475
Hpy99I CGWCG 5 cut(s) 48, 1379, 1415, 4782, 5119
HpyCH4IV ACGT 9 cut(s) 60, 696, 1377, 2374, 2567, 3495, 3631, 5733, 6746
HpySE526I ACGT 9 cut(s) 60, 696, 1377, 2374, 2567, 3495, 3631, 5733, 6746
Hsp92I GRCGYC 2 cut(s) 1377, 1410
HspAI GCGC 2 cut(s) 1361, 4775
Ksp22I TGATCA 3 cut(s) 1471, 2733, 5400
KspAI GTTAAC 1 cut(s) 5712
LguI GCTCTTC 4 cut(s) 1573, 2433, 2958, 4384
LmnI GCTCC 3 cut(s) 3271, 3631, 6550
MaeII ACGT 9 cut(s) 60, 696, 1377, 2374, 2567, 3495, 3631, 5733, 6746
MfeI CAATTG 9 cut(s) 207, 228, 840, 861, 2181, 2983, 3725, 4831, 6356
MflI RGATCY 9 cut(s) 799, 1629, 2499, 2545, 3086, 4431, 5304, 5350, 6538
MlsI TGGCCA 3 cut(s) 2371, 5102, 5173
MluNI TGGCCA 3 cut(s) 2371, 5102, 5173
MlyI GAGTC 4 cut(s) 1838, 3103, 3355, 5884
MmeI TCCRAC 4 cut(s) 3355, 4204, 6546, 6799
Mox20I TGGCCA 3 cut(s) 2371, 5102, 5173
Mph1103I ATGCAT 4 cut(s) 256, 2280, 2957, 5082
MroXI GAANNNNTTC 2 cut(s) 1859, 5744
MscI TGGCCA 3 cut(s) 2371, 5102, 5173
MslI CAYNNNNRTG 6 cut(s) 1604, 3200, 3924, 4251, 5996, 6228
Msp20I TGGCCA 3 cut(s) 2371, 5102, 5173
MspA1I CMGCKG 6 cut(s) 23, 436, 659, 677, 3389, 6506
MspI CCGG 7 cut(s) 422, 1055, 2290, 3311, 4701, 5918, 6475
MspR9I CCNGG 9 cut(s) 833, 1643, 2291, 2606, 3644, 3989, 5919, 6186, 6475
MunI CAATTG 9 cut(s) 207, 228, 840, 861, 2181, 2983, 3725, 4831, 6356
Mva1269I GAATGC 2 cut(s) 2488, 3289
MvaI CCWGG 6 cut(s) 833, 1643, 2606, 3644, 3989, 6186
MvnI CGCG 2 cut(s) 1993, 4777
NciI CCSGG 3 cut(s) 2291, 5919, 6475
NcoI CCATGG 9 cut(s) 1341, 1425, 1761, 3214, 4113, 4152, 4236, 4563, 6054
NdeI CATATG 2 cut(s) 3228, 3611
NlaIV GGNNCC 7 cut(s) 313, 3256, 3621, 4433, 6052, 6731, 6818
NmuCI GTSAC 7 cut(s) 944, 1378, 3041, 4189, 5938, 6085, 6255
NruI TCGCGA 1 cut(s) 1993
NsiI ATGCAT 4 cut(s) 256, 2280, 2957, 5082
NspI RCATGY 8 cut(s) 1439, 1451, 3297, 4151, 4250, 4698, 5638, 6026
PaeR7I CTCGAG 1 cut(s) 1974
PagI TCATGA 2 cut(s) 160, 1599
PciI ACATGT 3 cut(s) 1435, 3293, 4246
PciSI GCTCTTC 4 cut(s) 1573, 2433, 2958, 4384
PctI GAATGC 2 cut(s) 2488, 3289
PdmI GAANNNNTTC 2 cut(s) 1859, 5744
PflFI GACNNNGTC 1 cut(s) 1376
PflMI CCANNNNNTGG 1 cut(s) 3988
PfoI TCCNGGA 3 cut(s) 831, 1641, 2289
PinAI ACCGGT 1 cut(s) 3310
Ple19I CGATCG 1 cut(s) 757
PleI GAGTC 4 cut(s) 1838, 3102, 3354, 5884
PmaCI CACGTG 1 cut(s) 3632
PmlI CACGTG 1 cut(s) 3632
PpsI GAGTC 4 cut(s) 1838, 3102, 3354, 5884
Ppu21I YACGTR 1 cut(s) 3632
PpuMI RGGWCCY 2 cut(s) 3620, 3733
PscI ACATGT 3 cut(s) 1435, 3293, 4246
PshBI ATTAAT 2 cut(s) 2408, 3111
PsiI TTATAA 4 cut(s) 1128, 1637, 4439, 5879
Psp124BI GAGCTC 1 cut(s) 4396
Psp1406I AACGTT 1 cut(s) 5733
Psp5II RGGWCCY 2 cut(s) 3620, 3733
Psp6I CCWGG 6 cut(s) 831, 1641, 2604, 3642, 3987, 6184
PspCI CACGTG 1 cut(s) 3632
PspFI CCCAGC 1 cut(s) 4490
PspGI CCWGG 6 cut(s) 831, 1641, 2604, 3642, 3987, 6184
PspN4I GGNNCC 7 cut(s) 313, 3256, 3621, 4433, 6052, 6731, 6818
PspOMI GGGCCC 2 cut(s) 6050, 6816
PspPPI RGGWCCY 2 cut(s) 3620, 3733
PspXI VCTCGAGB 1 cut(s) 1974
PstI CTGCAG 3 cut(s) 129, 3601, 6270
PstNI CAGNNNCTG 3 cut(s) 1574, 2635, 3956
PsuI RGATCY 9 cut(s) 799, 1629, 2499, 2545, 3086, 4431, 5304, 5350, 6538
PsyI GACNNNGTC 1 cut(s) 1376
PvuI CGATCG 1 cut(s) 757
PvuII CAGCTG 6 cut(s) 23, 436, 659, 677, 3389, 6506
RruI TCGCGA 1 cut(s) 1993
RseI CAYNNNNRTG 6 cut(s) 1604, 3200, 3924, 4251, 5996, 6228
SacI GAGCTC 1 cut(s) 4396
SalI GTCGAC 1 cut(s) 974
SapI GCTCTTC 4 cut(s) 1573, 2433, 2958, 4384
ScaI AGTACT 1 cut(s) 6679
SchI GAGTC 4 cut(s) 1838, 3103, 3355, 5884
ScrFI CCNGG 9 cut(s) 833, 1643, 2291, 2606, 3644, 3989, 5919, 6186, 6475
SfcI CTRYAG 5 cut(s) 125, 2474, 3597, 5960, 6266
Sfr274I CTCGAG 1 cut(s) 1974
SinI GGWCC 9 cut(s) 430, 1063, 1815, 3254, 3421, 3620, 3733, 4617, 6425
SlaI CTCGAG 1 cut(s) 1974
SmiI ATTTAAAT 2 cut(s) 2493, 3189
SmiMI CAYNNNNRTG 6 cut(s) 1604, 3200, 3924, 4251, 5996, 6228
SmlI CTYRAG 8 cut(s) 1510, 1974, 2672, 2969, 3091, 4321, 5480, 6344
SmoI CTYRAG 8 cut(s) 1510, 1974, 2672, 2969, 3091, 4321, 5480, 6344
SsiI CCGC 6 cut(s) 550, 560, 4061, 5871, 6182, 6672
SspI AATATT 5 cut(s) 2308, 2926, 5315, 5692, 6521
SstI GAGCTC 1 cut(s) 4396
StyD4I CCNGG 9 cut(s) 831, 1641, 2289, 2604, 3642, 3987, 5917, 6184, 6473
SwaI ATTTAAAT 2 cut(s) 2493, 3189
TaiI ACGT 9 cut(s) 63, 699, 1380, 2377, 2570, 3498, 3634, 5736, 6749
TauI GCSGC 1 cut(s) 6184
TseFI GTSAC 7 cut(s) 944, 1378, 3041, 4189, 5938, 6085, 6255
Tsp45I GTSAC 7 cut(s) 944, 1378, 3041, 4189, 5938, 6085, 6255
TspGWI ACGGA 6 cut(s) 1177, 1252, 2031, 4224, 5079, 6031
Tth111I GACNNNGTC 1 cut(s) 1376
Van91I CCANNNNNTGG 1 cut(s) 3988
VneI GTGCAC 2 cut(s) 5184, 6599
VpaK11BI GGWCC 9 cut(s) 430, 1063, 1815, 3254, 3421, 3620, 3733, 4617, 6425
VspI ATTAAT 2 cut(s) 2408, 3111
XbaI TCTAGA 3 cut(s) 2617, 3515, 5422
XceI RCATGY 8 cut(s) 1439, 1451, 3297, 4151, 4250, 4698, 5638, 6026
XcmI CCANNNNNNNNNTGG 1 cut(s) 3265
XhoI CTCGAG 1 cut(s) 1974
XmiI GTMKAC 5 cut(s) 975, 1903, 3744, 4018, 4206
XmnI GAANNNNTTC 2 cut(s) 1859, 5744
ZraI GACGTC 1 cut(s) 1378
ZrmI AGTACT 1 cut(s) 6679
Zsp2I ATGCAT 4 cut(s) 256, 2280, 2957, 5082
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.