Rroxscaffold_1G00003430

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000085
Physical Location & Seq
Reverse (-)
4169422 .. 4173317
3896 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00003430.1

Sequence Viewer

Length: 3420 bp
ATGGCCTTGTGCCCCCAATCAGCCTCTGCATCTTTTCCTTCATCATCAGCTCCTAGATCAAAGCATGATGTGTTTCTCAGTTTTAGGGGTGAAGACACTCGCAAGGGTGTTGTATCCTATTTATACCATAAATTGGAAAACAGAGGAATACAAACATTCATGGATGACCAAGGGCTTCAGCAAGGGATGCCTATTTCTCCAAGTCTCGTAGCTGCAATAGAGGAATCGAGGTATGCAATCGTTCTTCTGTCTCCAAACTATGCTTCTTCTACATGGTGTTTGGATGAACTTGTGAAGATTCTTCAATGCTCTGAAGCCAAGAAGACACCAGTTCTGCCAATTTTTTATTATGTGGATCCCTCTGATGTGCGAAATCAAAGGGGAAGTTTTGCAGAAGCCTTCACTAAGCATGAAGAGAGGTATAGGCAAGGCAATGATGATGTAGAGAAGGTGAAGAAGTGGAGAGCTGCTTTAACACAAGTGGCCAATCTCTCTGGGTTGGATTCAAACAAATTTGGGTCTGACAGAGAACTTGTCCAACGCATTGTGGAACTTGTGCGCCGTGATGTAAGGCCTATTTCAATAATGTCGAGCAGCAATTTTGAAGCATTTGAATCAACAAGAAAAGCCATGAATGAGCTGATGGAGGCATTAGAAGATGATGAGGTCGCTGCCATTGGGGTCTATGGAATGGGAGGGGTTGGGAAGACAACCATGGTGAAACATGTCGGTGCACAAGCCCAAAAAATGGGGCTTTTTGATCATGTGATTATGGCTGTTGTATCCCAAACCCCAGATAAAAGAAATATTCAAGGCGCACTTGCGGATCTGCTGGGCTTGAAATTGGTGGAGGAGACAGAAATTGGACGAGCCAGTAGATTGCAGAAGGAGATACTTAGAAGAAACAAGATCCTTATAATCTTAGACGACATTTGGAATAGACTGGAGTTGTCAAGCATTGGAATTCCCAGCTACAACGAGCTTCAAATATGCAATTCCAAAGTGCTACTCACCACAAGGAGATTGAATGTTTGCCATACCATGGAGAGCCAAGCAAAAATTCATCTCAGTATCTTATCAGAAGAAGATGCTTGGAACTTGTTTTTGAAGAAGGCAGGAAAGTCTTTTCAGAAGTCTACCGCTTTCTATGAAGTAGCCAGAAAGGTAGCTCGAGAATGTGCTGGTCTCCCAGTTGCGTTGATAGCAGTTGCGAGGGCACTTGGAGATAAAGATTTTGAAGCCTGGAAAGAAGCAGCTCGACAACTTGAGATGTCTCAACCTGCCAACCCTGAACAGGGCAAAGATGTGTTCAAGTGTATAAAGTTGAGCTATGACTACTTGGACGATAATGCCGATGTGTCCAGAAGTCAAGCCAAGTATTGCTTCTTGCTCTGCTGCCTATTCCCAGAAGATTATGATATCCAAATAGAAGACATTATGAAGTATGTCTTCAGAACAGGATTGTTCCGAGATGACAACAGCACAATAGAGAATGCCAGAGCCAACGCAAAATCAGTGATTGACCACCTTAAAGATTCTTGTTTGCTTTTGGACGGTTCAAAGGTCGGATGTATAAAGATGCACGATGTCATTCGAGATATTGCCGTGTCCATTGCATCAGATGGCCAATATTTTTTTGTGAAAACTCGTTGTGATTTAAAAGATTGGCCGATGGATGCGCATGAGGGCTACTCAGCAATCTCACTAATTGAGAGCAACATTTGCAAGCTTCCCAACAAGTTGGTATGTTCAAAACTTCAGATTTTATTGCTACAACAAAATGCTTGTATAATAAAGATCCCAAAAACCTTTTTTCAATGTCCCAATGATTTAAGGGTCTTAGATCTTAGCAATACTGGCATTTCATCACTGCCCCCATCATTCAGTCTCCTAACCACACTCCAAGCTTTGCAATTAGATAATTGCCAGTACCTTAGGGACATATCCATACTTAGAAAACTGAAAAAGCTCGAGATTCTTAGTATGAGAGACTGTTCTATTAAGAAATTTCCAAAAGAGATAGGAAATTTGACCAAATTAAGAATGTGGGACTTGACCAAAACTAGTTTTGAGACAGTTCCCTCTCAAGTGATAACAAGGTTGTATAGACTAGAAGAACTGTACATGAACTATAGTTTTGGCTACTGGGGTAGTAAAGTTAAGGGAGGCTTGTTGTGTTTTCTTGGTAAGAATGCTGACTTTGATGAGTTAACTGTCTTGTCACGTTTAAACACTTTGGAGGTTTCCGTATATGGTGTAGAATGCTTGCCTCAAAATGTTGAGCCCATTCCAAATTGGGTCAACTTTTATATACGTATCAGAAGATTCGGGTTCAGAAATTCCACTAGGGGAGATCACATTCCACATCCTTCTTCAAGAGCATTGATCATTGACACAACCATGAATAACTTACCCAATTGGTTCATCAGTGTGGTGACAGAAAAAACAGAGGATTTATGGTACAACTGCTGCAATGGCTTAACTAACCTTCTCGAGGAACACGACCGTGGGAGATTACATGGACTCAAGTATCTTTGTATAGAATCTGTATATGCAGAAGTGTTGTTCAACACAATAACATGGCCTAAGGGGTCAAATGAACCTGTGTTTGAGCACTTGGAAGAGTTGAAAATCTATTTCGAGCATTGCCTGGAGGAGCTATGTGTTGGTGATTTACCACCTGGCTCTGTACGTAATCTTAAGATATTGAATGTGCAATCTTGTTCTAGGTTGGTGAAACCACTTTTACCGTCAAAGTTGTTGCATAGATTACAGAGTCTGGAAAAACTAGTATGTTCTTATGTAGTTATGAATTATGTGTTTGGATTTGAAGGGGATGAACCACAACACGTTGTCTTTACAAAGTTGAGAATGATTTCACTGAGACAAATGTACCGGCTAATAAACATATGGAATGGTCCTGCTCCACCTGGAATCTTCCAGAGCCTCAAAATCTTTTACCTGAAGGATTGCCCAATACTGAAATATCTCTTCACATCTGAAGTAGCTCGATGTCTTTTGCAATTGGAAGACGTTTGGGTAGAGTGTTGTAGTAGATTGGACAGACTAATTGAAGCAACTGAGGAAACAGAGAGCAACAAGATTGTTCTTCCACAATTGAAAAACCTAGTTTTGAAACGTCTTCTAAAGCTCACAAGATTCTCCAGTACCAGCAGCAGTACTATTATTATTGAAATCGAGTGCCCTTCATTGCAACACTTGTACGTGGATGAGTGCCCCTGGGTTACAATCTCTGCTTCTGACTTCAGCAGCAGAAACCAAGTCCCCTGGTTTCCAATCTCTGCTTCTGGCATCAGCAGCAGAAACCAAGTCCAACTAAATGATCACCAACATATGGGCACACTACGGGGAAGGATCGACAATGAATCAAAGCTCTCACACTTTCCACTCTGGACGTGA

Protein Analysis

1139

Amino Acids

129.64

Weight (kDa)

7.42

Isoelectric Point (pI)

48.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 21 - 198 5.7e-52 TIR domain
TIR_2 PF13676 24 - 120 1.3e-16 TIR domain
NB-ARC PF00931 209 - 374 2e-31 NB-ARC domain
LRR_8 PF13855 585 - 643 6.9e-07 Leucine rich repeat
LRR_14 PF23598 637 - 930 1.8e-08 Leucine-rich repeat region
LRR_RPS2 PF23247 873 - 1017 3.4e-22 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 917
Acc16I TGCGCA 1 cut(s) 1680
Acc36I ACCTGC 1 cut(s) 1288
AccB7I CCANNNNNTGG 4 cut(s) 133, 748, 1042, 3355
AccI GTMKAC 1 cut(s) 1136
AciI CCGC 2 cut(s) 824, 1140
AclWI GGATC 6 cut(s) 350, 363, 834, 904, 1792, 3383
AcoI YGGCCR 3 cut(s) 483, 1624, 1667
AcsI RAATTY 6 cut(s) 512, 963, 1059, 2006, 2026, 2338
AcuI CTGAAG 7 cut(s) 161, 333, 1435, 1742, 2984, 3021, 3250
AfaI GTAC 8 cut(s) 1931, 2123, 2462, 2691, 2894, 3169, 3181, 3224
AfiI CCNNNNNNNGG 7 cut(s) 133, 748, 1042, 1294, 1295, 2494, 3355
AflII CTTAAG 1 cut(s) 2699
AflIII ACRYGT 2 cut(s) 724, 2848
AhlI ACTAGT 2 cut(s) 2063, 2788
AjiI CACGTC 1 cut(s) 3417
AjnI CCWGG 6 cut(s) 1241, 2649, 2680, 2929, 3239, 3287
AjuI GAANNNNNNNTTGG 8 cut(s) 247, 279, 1044, 1076, 1367, 1399, 3289, 3321
AleI CACNNNNGTG 1 cut(s) 2505
Alw21I GWGCWC 2 cut(s) 736, 2616
Alw26I GTCTC 9 cut(s) 209, 255, 848, 1190, 1278, 1892, 1983, 2066, 2878
Alw44I GTGCAC 1 cut(s) 732
AlwI GGATC 6 cut(s) 350, 363, 834, 904, 1792, 3383
AlwNI CAGNNNCTG 2 cut(s) 26, 2779
Ama87I CYCGRG 3 cut(s) 1170, 1970, 2492
AoxI GGCC 6 cut(s) 3, 483, 572, 1624, 1667, 2582
ApaLI GTGCAC 1 cut(s) 732
ApoI RAATTY 6 cut(s) 512, 963, 1059, 2006, 2026, 2338
Asp700I GAANNNNTTC 1 cut(s) 2875
AspLEI GCGC 3 cut(s) 561, 818, 1681
AspS9I GGNCC 1 cut(s) 2918
AsuHPI GGTGA 8 cut(s) 101, 463, 730, 1003, 2446, 2681, 2746, 3338
AvaI CYCGRG 3 cut(s) 1170, 1970, 2492
AvaII GGWCC 1 cut(s) 2918
AxyI CCTNAGG 2 cut(s) 1934, 2586
BaeGI GKGCMC 6 cut(s) 14, 736, 1219, 3206, 3239, 3362
BaeI ACNNNNGTAYC 4 cut(s) 2513, 2546, 2876, 2909
BalI TGGCCA 2 cut(s) 485, 1626
BamHI GGATCC 1 cut(s) 355
BanII GRGCYC 1 cut(s) 2286
BbsI GAAGAC 7 cut(s) 99, 329, 713, 1437, 1441, 3036, 3134
Bbv12I GWGCWC 2 cut(s) 736, 2616
BccI CCATC 4 cut(s) 637, 1616, 1666, 1885
BceAI ACGGC 2 cut(s) 546, 1589
BciT130I CCWGG 6 cut(s) 1243, 2651, 2682, 2931, 3241, 3289
BciVI GTATCC 2 cut(s) 124, 793
BclI TGATCA 3 cut(s) 760, 2385, 3343
BcoDI GTCTC 9 cut(s) 209, 255, 848, 1190, 1278, 1892, 1983, 2066, 2878
BcuI ACTAGT 2 cut(s) 2063, 2788
BfaI CTAG 7 cut(s) 54, 2064, 2111, 2346, 2727, 2789, 3128
BfmI CTRYAG 1 cut(s) 2131
BfrI CTTAAG 1 cut(s) 2699
BfuAI ACCTGC 1 cut(s) 1288
BfuI GTATCC 2 cut(s) 124, 793
BglII AGATCT 1 cut(s) 1843
BmcAI AGTACT 1 cut(s) 3181
Bme1390I CCNGG 6 cut(s) 1243, 2651, 2682, 2931, 3241, 3289
Bme18I GGWCC 1 cut(s) 2918
BmeT110I CYCGRG 3 cut(s) 1170, 1970, 2492
BmgBI CACGTC 1 cut(s) 3417
BmgT120I GGNCC 1 cut(s) 2918
BmiI GGNNCC 1 cut(s) 357
BmrFI CCNGG 6 cut(s) 1243, 2651, 2682, 2931, 3241, 3289
BmrI ACTGGG 2 cut(s) 1184, 2155
BmsI GCATC 7 cut(s) 38, 177, 1078, 1569, 1625, 1666, 3321
BmuI ACTGGG 2 cut(s) 1184, 2155
BpiI GAAGAC 7 cut(s) 99, 329, 713, 1437, 1441, 3036, 3134
BplI GAGNNNNNCTC 2 cut(s) 1676, 1708
BpmI CTGGAG 3 cut(s) 965, 2672, 3148
BpuEI CTTGAG 3 cut(s) 1286, 2070, 2510
BsaAI YACGTR 3 cut(s) 2315, 2693, 3226
BsaI GGTCTC 1 cut(s) 1190
BsaJI CCNNGG 7 cut(s) 169, 714, 1041, 2506, 3239, 3240, 3287
Bsc4I CCNNNNNNNGG 7 cut(s) 133, 748, 1042, 1294, 1295, 2494, 3355
Bse118I RCCGGY 1 cut(s) 2895
Bse1I ACTGG 8 cut(s) 329, 873, 948, 1190, 1861, 1927, 2150, 3165
Bse21I CCTNAGG 2 cut(s) 1934, 2586
Bse3DI GCAATG 5 cut(s) 439, 1611, 2479, 2644, 3209
BseBI CCWGG 6 cut(s) 1243, 2651, 2682, 2931, 3241, 3289
BseDI CCNNGG 7 cut(s) 169, 714, 1041, 2506, 3239, 3240, 3287
BseGI GGATG 8 cut(s) 169, 192, 289, 1574, 1681, 2365, 2842, 3235
BseLI CCNNNNNNNGG 7 cut(s) 133, 748, 1042, 1294, 1295, 2494, 3355
BseMI GCAATG 5 cut(s) 439, 1611, 2479, 2644, 3209
BseMII CTCAG 5 cut(s) 91, 1081, 1707, 2873, 3072
BseNI ACTGG 8 cut(s) 329, 873, 948, 1190, 1861, 1927, 2150, 3165
BseRI GAGGAG 2 cut(s) 866, 2669
BseSI GKGCMC 6 cut(s) 14, 736, 1219, 3206, 3239, 3362
BseYI CCCAGC 2 cut(s) 832, 968
Bsh1285I CGRYCG 1 cut(s) 2506
BshFI GGCC 6 cut(s) 5, 485, 574, 1626, 1669, 2584
BsiEI CGRYCG 1 cut(s) 2506
BsiHKAI GWGCWC 2 cut(s) 736, 2616
BsiHKCI CYCGRG 3 cut(s) 1170, 1970, 2492
BsiSI CCGG 1 cut(s) 2896
BslFI GGGAC 4 cut(s) 1806, 1952, 2063, 3269
BslI CCNNNNNNNGG 7 cut(s) 133, 748, 1042, 1294, 1295, 2494, 3355
BsmAI GTCTC 9 cut(s) 209, 255, 848, 1190, 1278, 1892, 1983, 2066, 2878
BsmFI GGGAC 4 cut(s) 1806, 1952, 2063, 3269
BsmI GAATGC 3 cut(s) 1498, 2197, 2267
BsnI GGCC 6 cut(s) 5, 485, 574, 1626, 1669, 2584
Bso31I GGTCTC 1 cut(s) 1190
BsoBI CYCGRG 3 cut(s) 1170, 1970, 2492
Bsp1286I GDGCHC 8 cut(s) 14, 736, 1219, 2286, 2616, 3206, 3239, 3362
Bsp1407I TGTACA 1 cut(s) 2121
Bsp19I CCATGG 2 cut(s) 714, 1041
BspACI CCGC 2 cut(s) 824, 1140
BspANI GGCC 6 cut(s) 5, 485, 574, 1626, 1669, 2584
BspCNI CTCAG 5 cut(s) 90, 1080, 1706, 2874, 3073
BspLI GGNNCC 1 cut(s) 357
BspMI ACCTGC 1 cut(s) 1288
BspPI GGATC 6 cut(s) 350, 363, 834, 904, 1792, 3383
BspTI CTTAAG 1 cut(s) 2699
BspTNI GGTCTC 1 cut(s) 1190
BsrDI GCAATG 5 cut(s) 439, 1611, 2479, 2644, 3209
BsrFI RCCGGY 1 cut(s) 2895
BsrGI TGTACA 1 cut(s) 2121
BsrI ACTGG 8 cut(s) 329, 873, 948, 1190, 1861, 1927, 2150, 3165
BssAI RCCGGY 1 cut(s) 2895
BssECI CCNNGG 7 cut(s) 169, 714, 1041, 2506, 3239, 3240, 3287
BssT1I CCWWGG 3 cut(s) 169, 714, 1041
Bst2UI CCWGG 6 cut(s) 1243, 2651, 2682, 2931, 3241, 3289
Bst4CI ACNGT 7 cut(s) 1556, 1994, 2077, 2121, 2215, 2507, 2751
Bst6I CTCTTC 3 cut(s) 408, 2616, 2996
BstAFI CTTAAG 1 cut(s) 2699
BstAPI GCANNNNNTGC 2 cut(s) 187, 1722
BstAUI TGTACA 1 cut(s) 2121
BstBAI YACGTR 3 cut(s) 2315, 2693, 3226
BstC8I GCNNGC 2 cut(s) 1727, 2267
BstDSI CCRYGG 3 cut(s) 714, 1041, 2506
BstENI CCTNNNNNAGG 1 cut(s) 2492
BstF5I GGATG 8 cut(s) 169, 192, 289, 1574, 1681, 2365, 2842, 3235
BstHHI GCGC 3 cut(s) 561, 818, 1681
BstMAI GTCTC 9 cut(s) 209, 255, 848, 1190, 1278, 1892, 1983, 2066, 2878
BstMCI CGRYCG 1 cut(s) 2506
BstMWI GCNNNNNNNGC 6 cut(s) 187, 1202, 1722, 1857, 2475, 3318
BstNI CCWGG 6 cut(s) 1243, 2651, 2682, 2931, 3241, 3289
BstNSI RCATGY 1 cut(s) 728
BstSCI CCNGG 6 cut(s) 1241, 2649, 2680, 2929, 3239, 3287
BstSFI CTRYAG 1 cut(s) 2131
BstSLI GKGCMC 6 cut(s) 14, 736, 1219, 3206, 3239, 3362
BstSNI TACGTA 2 cut(s) 2315, 2693
BstV2I GAAGAC 7 cut(s) 99, 329, 713, 1437, 1441, 3036, 3134
BstX2I RGATCY 5 cut(s) 355, 826, 909, 1797, 1843
BstXI CCANNNNNNTGG 1 cut(s) 1741
BstYI RGATCY 5 cut(s) 355, 826, 909, 1797, 1843
Bsu36I CCTNAGG 2 cut(s) 1934, 2586
BsuI GTATCC 2 cut(s) 124, 793
BsuRI GGCC 6 cut(s) 5, 485, 574, 1626, 1669, 2584
BtgI CCRYGG 3 cut(s) 714, 1041, 2506
BtrI CACGTC 1 cut(s) 3417
BtsCI GGATG 8 cut(s) 169, 192, 289, 1574, 1681, 2365, 2842, 3235
BtsI GCAGTG 1 cut(s) 1868
BtsIMutI CAGTG 4 cut(s) 1521, 1868, 2434, 2879
BveI ACCTGC 1 cut(s) 1288
Cac8I GCNNGC 2 cut(s) 1727, 2267
CaiI CAGNNNCTG 2 cut(s) 26, 2779
CfoI GCGC 3 cut(s) 561, 818, 1681
Cfr10I RCCGGY 1 cut(s) 2895
Cfr13I GGNCC 1 cut(s) 2918
Csp6I GTAC 8 cut(s) 1930, 2122, 2461, 2690, 2893, 3168, 3180, 3223
CviQI GTAC 8 cut(s) 1930, 2122, 2461, 2690, 2893, 3168, 3180, 3223
DraI TTTAAA 2 cut(s) 1659, 2229
EaeI YGGCCR 3 cut(s) 483, 1624, 1667
Eam1104I CTCTTC 3 cut(s) 408, 2616, 2996
EarI CTCTTC 3 cut(s) 408, 2616, 2996
Eco105I TACGTA 2 cut(s) 2315, 2693
Eco130I CCWWGG 3 cut(s) 169, 714, 1041
Eco147I AGGCCT 1 cut(s) 574
Eco24I GRGCYC 1 cut(s) 2286
Eco31I GGTCTC 1 cut(s) 1190
Eco32I GATATC 1 cut(s) 1420
Eco47I GGWCC 1 cut(s) 2918
Eco57I CTGAAG 7 cut(s) 161, 333, 1435, 1742, 2984, 3021, 3250
Eco81I CCTNAGG 2 cut(s) 1934, 2586
Eco88I CYCGRG 3 cut(s) 1170, 1970, 2492
EcoNI CCTNNNNNAGG 1 cut(s) 2492
EcoRI GAATTC 1 cut(s) 963
EcoRII CCWGG 6 cut(s) 1241, 2649, 2680, 2929, 3239, 3287
EcoRV GATATC 1 cut(s) 1420
EcoT14I CCWWGG 3 cut(s) 169, 714, 1041
EcoT38I GRGCYC 1 cut(s) 2286
ErhI CCWWGG 3 cut(s) 169, 714, 1041
FalI AAGNNNNNCTT 8 cut(s) 804, 836, 1367, 1399, 1433, 1465, 2153, 2185
FaqI GGGAC 4 cut(s) 1806, 1952, 2063, 3269
FauNDI CATATG 2 cut(s) 2909, 3354
FbaI TGATCA 3 cut(s) 760, 2385, 3343
FblI GTMKAC 1 cut(s) 1136
FokI GGATG 8 cut(s) 176, 199, 296, 1581, 1688, 2352, 2849, 3242
FriOI GRGCYC 1 cut(s) 2286
FspAI RTGCGCAY 1 cut(s) 1680
FspBI CTAG 7 cut(s) 54, 2064, 2111, 2346, 2727, 2789, 3128
FspI TGCGCA 1 cut(s) 1680
GlaI GCGC 3 cut(s) 560, 817, 1680
GsaI CCCAGC 2 cut(s) 836, 972
GsuI CTGGAG 3 cut(s) 965, 2672, 3148
HaeIII GGCC 6 cut(s) 5, 485, 574, 1626, 1669, 2584
HapII CCGG 1 cut(s) 2896
HhaI GCGC 3 cut(s) 561, 818, 1681
Hin6I GCGC 3 cut(s) 559, 816, 1679
HinP1I GCGC 3 cut(s) 559, 816, 1679
HincII GTYRAC 2 cut(s) 2211, 2302
HindII GTYRAC 2 cut(s) 2211, 2302
HindIII AAGCTT 2 cut(s) 1727, 1905
HpaI GTTAAC 1 cut(s) 2211
HpaII CCGG 1 cut(s) 2896
HphI GGTGA 8 cut(s) 101, 463, 730, 1003, 2446, 2681, 2746, 3338
Hpy166II GTNNAC 4 cut(s) 734, 1137, 2211, 2302
Hpy188III TCNNGA 9 cut(s) 1172, 1362, 1595, 1972, 2376, 2492, 2780, 2941, 3412
Hpy8I GTNNAC 4 cut(s) 734, 1137, 2211, 2302
HpyCH4III ACNGT 7 cut(s) 1556, 1994, 2077, 2121, 2215, 2507, 2751
HpyCH4IV ACGT 8 cut(s) 2224, 2314, 2692, 2850, 3033, 3139, 3225, 3416
HpyF10VI GCNNNNNNNGC 6 cut(s) 187, 1202, 1722, 1857, 2475, 3318
HpySE526I ACGT 8 cut(s) 2224, 2314, 2692, 2850, 3033, 3139, 3225, 3416
HspAI GCGC 3 cut(s) 559, 816, 1679
Ksp22I TGATCA 3 cut(s) 760, 2385, 3343
KspAI GTTAAC 1 cut(s) 2211
LmnI GCTCC 3 cut(s) 55, 2656, 2929
LweI GCATC 7 cut(s) 38, 177, 1078, 1569, 1625, 1666, 3321
MaeI CTAG 7 cut(s) 54, 2064, 2111, 2346, 2727, 2789, 3128
MaeII ACGT 8 cut(s) 2224, 2314, 2692, 2850, 3033, 3139, 3225, 3416
MaeIII GTNAC 3 cut(s) 2220, 2434, 3244
MfeI CAATTG 3 cut(s) 2416, 3023, 3116
MflI RGATCY 5 cut(s) 355, 826, 909, 1797, 1843
MhlI GDGCHC 8 cut(s) 14, 736, 1219, 2286, 2616, 3206, 3239, 3362
MlsI TGGCCA 2 cut(s) 485, 1626
MluNI TGGCCA 2 cut(s) 485, 1626
MlyI GAGTC 2 cut(s) 2517, 2785
MmeI TCCRAC 4 cut(s) 480, 562, 1546, 3358
Mox20I TGGCCA 2 cut(s) 485, 1626
MroXI GAANNNNTTC 1 cut(s) 2875
MscI TGGCCA 2 cut(s) 485, 1626
MslI CAYNNNNRTG 4 cut(s) 729, 2399, 2429, 2505
Msp20I TGGCCA 2 cut(s) 485, 1626
MspCI CTTAAG 1 cut(s) 2699
MspI CCGG 1 cut(s) 2896
MspR9I CCNGG 6 cut(s) 1243, 2651, 2682, 2931, 3241, 3289
MssI GTTTAAAC 1 cut(s) 2229
MunI CAATTG 3 cut(s) 2416, 3023, 3116
Mva1269I GAATGC 3 cut(s) 1498, 2197, 2267
MvaI CCWGG 6 cut(s) 1243, 2651, 2682, 2931, 3241, 3289
MwoI GCNNNNNNNGC 6 cut(s) 187, 1202, 1722, 1857, 2475, 3318
NcoI CCATGG 2 cut(s) 714, 1041
NdeI CATATG 2 cut(s) 2909, 3354
NlaIV GGNNCC 1 cut(s) 357
NmuCI GTSAC 2 cut(s) 2220, 2434
NsbI TGCGCA 1 cut(s) 1680
NspI RCATGY 1 cut(s) 728
OliI CACNNNNGTG 1 cut(s) 2505
PaeR7I CTCGAG 3 cut(s) 1170, 1970, 2492
PasI CCCWGGG 1 cut(s) 3240
PceI AGGCCT 1 cut(s) 574
PciI ACATGT 1 cut(s) 724
PcsI WCGNNNNNNNCGW 2 cut(s) 1350, 2499
PctI GAATGC 3 cut(s) 1498, 2197, 2267
PdmI GAANNNNTTC 1 cut(s) 2875
PflMI CCANNNNNTGG 4 cut(s) 133, 748, 1042, 3355
PleI GAGTC 2 cut(s) 2517, 2784
PmeI GTTTAAAC 1 cut(s) 2229
PpsI GAGTC 2 cut(s) 2517, 2784
Ppu21I YACGTR 3 cut(s) 2315, 2693, 3226
PscI ACATGT 1 cut(s) 724
PsiI TTATAA 1 cut(s) 917
Psp6I CCWGG 6 cut(s) 1241, 2649, 2680, 2929, 3239, 3287
PspFI CCCAGC 2 cut(s) 832, 968
PspGI CCWGG 6 cut(s) 1241, 2649, 2680, 2929, 3239, 3287
PspN4I GGNNCC 1 cut(s) 357
PspPI GGNCC 1 cut(s) 2918
PstNI CAGNNNCTG 2 cut(s) 26, 2779
PsuI RGATCY 5 cut(s) 355, 826, 909, 1797, 1843
RsaI GTAC 8 cut(s) 1931, 2123, 2462, 2691, 2894, 3169, 3181, 3224
RsaNI GTAC 8 cut(s) 1930, 2122, 2461, 2690, 2893, 3168, 3180, 3223
RseI CAYNNNNRTG 4 cut(s) 729, 2399, 2429, 2505
Sau96I GGNCC 1 cut(s) 2918
ScaI AGTACT 1 cut(s) 3181
SchI GAGTC 2 cut(s) 2517, 2785
ScrFI CCNGG 6 cut(s) 1243, 2651, 2682, 2931, 3241, 3289
SduI GDGCHC 8 cut(s) 14, 736, 1219, 2286, 2616, 3206, 3239, 3362
SfaNI GCATC 7 cut(s) 38, 177, 1078, 1569, 1625, 1666, 3321
SfcI CTRYAG 1 cut(s) 2131
Sfr274I CTCGAG 3 cut(s) 1170, 1970, 2492
SinI GGWCC 1 cut(s) 2918
SlaI CTCGAG 3 cut(s) 1170, 1970, 2492
SmiMI CAYNNNNRTG 4 cut(s) 729, 2399, 2429, 2505
SmlI CTYRAG 7 cut(s) 1170, 1265, 1970, 2085, 2492, 2525, 2699
SmoI CTYRAG 7 cut(s) 1170, 1265, 1970, 2085, 2492, 2525, 2699
SnaBI TACGTA 2 cut(s) 2315, 2693
SpeI ACTAGT 2 cut(s) 2063, 2788
SseBI AGGCCT 1 cut(s) 574
SsiI CCGC 2 cut(s) 824, 1140
SspI AATATT 2 cut(s) 808, 1631
SspMI CTAG 7 cut(s) 54, 2064, 2111, 2346, 2727, 2789, 3128
StuI AGGCCT 1 cut(s) 574
StyD4I CCNGG 6 cut(s) 1241, 2649, 2680, 2929, 3239, 3287
StyI CCWWGG 3 cut(s) 169, 714, 1041
TaaI ACNGT 7 cut(s) 1556, 1994, 2077, 2121, 2215, 2507, 2751
TaiI ACGT 8 cut(s) 2227, 2317, 2695, 2853, 3036, 3142, 3228, 3419
TatI WGTACW 2 cut(s) 2121, 3179
TscAI CASTG 4 cut(s) 1521, 1875, 2434, 2886
TseFI GTSAC 2 cut(s) 2220, 2434
Tsp45I GTSAC 2 cut(s) 2220, 2434
TspGWI ACGGA 1 cut(s) 2236
TspRI CASTG 4 cut(s) 1521, 1875, 2434, 2886
Van91I CCANNNNNTGG 4 cut(s) 133, 748, 1042, 3355
Vha464I CTTAAG 1 cut(s) 2699
VneI GTGCAC 1 cut(s) 732
VpaK11BI GGWCC 1 cut(s) 2918
XagI CCTNNNNNAGG 1 cut(s) 2492
XapI RAATTY 6 cut(s) 512, 963, 1059, 2006, 2026, 2338
XceI RCATGY 1 cut(s) 728
XhoI CTCGAG 3 cut(s) 1170, 1970, 2492
XmiI GTMKAC 1 cut(s) 1136
XmnI GAANNNNTTC 1 cut(s) 2875
XspI CTAG 7 cut(s) 54, 2064, 2111, 2346, 2727, 2789, 3128
ZrmI AGTACT 1 cut(s) 3181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.