Rw6G007080
WRKY Family

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000085
Physical Location & Seq
Reverse (-)
11471807 .. 11477523
5717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G007080.1

Sequence Viewer

Length: 3288 bp
ATGGCGGAGATTGTTTTGGGAATTCTTGGCCCAGTGATGCAACTTGGTGAGTGGATGTGGACTCCTGTAGAGCGTGGGATGAGTCACATGGTTCATTACAAGAAAAACTTACAGTCTCTTGACAGTCAATTTAAAAGGCTTGAGGCGAAGTGGAATGATCATAAAGAATCTGTGGATAGTGCTGAAACGAGTGGAGACAAAATCAAGCATGAGGTTCGAGCATGGCTAACAGAAGCTGATAATGCCATGAAAGATGTGAGAGAATTGAATGGTGAAGCTGGAGCAGGTGAAAAGTGCTTTATAGGATGGTGTCCGGATTTGAAATGGCGTTACAAGTTGGGGAAGAGAGCATGTATTCTGACAACAACACTAAAGAACCTGGTAGAAGAGGCAAAATTTGAGACTGTTACACTTGAAGTTCGACGAGCACCTGGAGTTCAGCACTTGATCTCCACTGAAGGATTTGAAGAATTTGAAGCAACCAGACAAGCCATGGATAGAGTCATGAACGCGCTGGAGAAAGATGAGGTCACAGCCATTGGTGTCTATGGAATGGGTGGCATAGGGAAAACAACTCTGGTGAAACACGTTGGTGCACAAGTGTGTAAAGATGGGCTCTTTGATCTTATGATTATGGCTGTTGTCTCTCAGAACCCCGTGGTGATGCAAATTCAAGACCAACTTAAAGATATGCTGGCATTGAAGTTACATGAAACAACAGAAATGGCAAGAGCAGCTAGACTGAGGGAGAGGATTATGAGAGGAAAGAAGATCCTCATAATCTTGGATGATATCTGGGACACAATAGATTTGTCTTTCATAGGAATTCCTAGCCACGACGAGCTTGGAAGATGCGGTTCAAAAGTCATCCTTACCACAAGGATATTCCACATTTGTCATACCATGGGCAGTCAAGTTAAGATCCCTCTCAACCGATTATCAGAAGATGATTCTTGGAATTTGTTTGTGAGGAATGCTAGAAAGTCTTTCGAGTCTACCAATCTCTGTGAGTTAGCAAAGCAAGTAGCTAAAGAGTGTGGTGGCCTCCCAGTTGCATTGATCACAGTTGCAAGGGCACTTGGAGATAAACACCAGTTGGAAGAATGGCAAGAAGCAGCTCGACGACTAGAAATTTCCCAACCTGCAAGTCTTGATGATGACGGACGTGTGCTCAAATGTATAAGGTTAAGTTATGATTACTTGAAAGGCGAGGATGTAAAATCATTCTTCTTGCTTTGTTGCCTCTTTCCAGAAGACTATGATATCCTAATAGAGGACTTGATCAAGTATGGGCTTGGGAAAGGGTTATTTCGGGATGCTGCCACATTTGAAGAAGCCAGAAGTACAGTGCATTCGGTGATCAACTACCTTAAATCTTCCAACTTGCTTTTGGACAGTAAAAGGGATGAATGTGTAAGGTTGCATGATGTCATTCGGGATATGGCCATGTTAATTGCATCATCCGAATATGGTGATAGATTTTTGGTTAAAGCTCGGATTCAAATAAGGGTTTGGCCAAATAATGCATATGAAGGCTGCTCTGCAATCTCTTTAATGGACAACGATATTCAGAAGCTTCCTGAAGAGCTGGCATGTCCAAAACTTCATATTCTACTACTGCAAAATAATACAAATATGACGGAGATCCCAGAAACATTTTTTGAAAGTCTGAATGAGTTGAAGGTCTTAGATCTCAGTCGCTGTGGTATGTCGCACTTACCACACTCTTTGAGTCTGCGGACCAACCTCCAGGCATTATATTTGGATAATTGCCGGTCAATAAGGTATGTTTCTCTGCTCGGACAGCTGAAGAAACTTGAAATTCTTAGCCTGAGACATTCAGCCCTTGAAGAATTACCTGAAGAAATAGGAAATTTGGCTAAGCTAAGAATGCTTGATCTTAGTGGGAGTGGGTCCATTAGGAGAATTCCATCCAATTTGATATCAAGGTTGTCTAGGTTAGAAGAACTATACATGCAAGGTAGTTTTCAAGATTGGGGAAGTAAAGTTGAGGGAGAAGGAGAAGAAACCAATGTTGGATTTGATGAGTTGATTTCCCTGTCATACTTGAATATTTTGGAGGTTGACATAATTGATCCTAAGTGTTTGCCAAACTATGTTAGCTCTAATCCCAATTGGGTAAAGTTTCACATAGGTATCAGAGGAAACGCACGTGGGCCTCTGGTTATCTCTCTTTGGGAGCTTCTTTGGATAAATATTTATAAGTTAGATTCAAGAGCATTGAACCTTAACACAAGCATTACCACCTTGCCCGGTTGGTTTAATAGTGCTGTTACCCAGAGAGCCAAGAAAGTTAGGTACACAAATTGCGAGGGCTTAAATAACATTCTAGTGGAATATGACCATGGAAATTTACTGAGACTTCAGTCTCTAGTTATCCAAGCTTGCTATCAAGTTGTAACTTTGATGGACACAACTACAAGGGTTCCAATCACGAATAAACCTATGTTTGAGAATCTGGAATTCTTGTGGGTGGTGAATTTGAATAATTTGAAAGAGTTGTGTGTTGGTGAATTACCACAAGGGTCTCTGGGCAATCTGAAGTATTTGGAGGCGAACAATTGTAGTAACTTGTTGAATCCACTTCTTCCATCAAATTTGTTGAAAAGATTGCAAAATCTGGAGACACTTGTTATTTCAGATGTATCACGTGCGGAATATGTGTTTCGGTCAGATGATTTTGGAGAAGAACAAATTGAGTTGAGAAAATTTAGAGATCTCAGATTGTGCCGTCTACGGCAACTGGAAAACATATGGAGCGGTCCTGTTGGTTATGTAATCTTCTGTAATCTTCGACGTTTGGAAGTTGACAGCTGCGGCAAATTGAAATATCTCTTCACATCTGATGTATCTCAAAGTCTCTTGCAATTGGAGTATCTTGTTGTAGACTCCTGTGATTGTTTGGAGATGATCATTGCACCAGGCAAGGGAACAGTGAACAAAATAGTTTTGCCACAGTTAAATTACTTATGTTTGGAGGATCTTCCACAGCTCACAAGCTTCTACACTGGAACTAACGGAATTGAGTGTCCGTCATTGGAACTCTTATCTGTGCACGAGTGCCCACAGTTTCCAACCTCTACTTACAACTTCCATAGTAGAAACCAAGTCAATGTCCGTTCGAGACACTTTCATAAGTTTTGTATGACAATTCCATGGTTGTATGTTCATGAAGAGCCATTAGTTCTAAATCATTCCAAACACAGTCGTTCCCAACATCCTTTACTTGGTATATATAGCTGCAGGTCTTCATTCTCGAGCATGTAA

Protein Analysis

1095

Amino Acids

124.63

Weight (kDa)

6.04

Isoelectric Point (pI)

44.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 163 - 326 2.7e-31 NB-ARC domain
LRR_8 PF13855 533 - 592 7.2e-07 Leucine rich repeat
LRR_14 PF23598 597 - 694 4.3e-09 Leucine-rich repeat region
LRR_8 PF13855 605 - 661 7.4e-06 Leucine rich repeat
LRR_RPS2 PF23247 832 - 973 9.5e-30 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2223
AarI CACCTGC 1 cut(s) 275
Acc36I ACCTGC 3 cut(s) 275, 1150, 3255
AccBSI CCGCTC 1 cut(s) 2781
AccI GTMKAC 3 cut(s) 995, 2755, 2907
AccII CGCG 1 cut(s) 512
AccIII TCCGGA 1 cut(s) 313
AciI CCGC 6 cut(s) 5, 855, 1738, 2675, 2781, 2838
AclWI GGATC 5 cut(s) 766, 916, 1639, 2090, 3009
AcoI YGGCCR 2 cut(s) 1443, 1514
AcuI CTGAAG 6 cut(s) 477, 1602, 1829, 1881, 2369, 2582
AcvI CACGTG 2 cut(s) 2174, 2672
AfaI GTAC 2 cut(s) 1345, 2321
AfiI CCNNNNNNNGG 3 cut(s) 1273, 2948, 3248
AflIII ACRYGT 2 cut(s) 586, 1165
AjiI CACGTC 1 cut(s) 1166
AjnI CCWGG 4 cut(s) 378, 430, 1749, 2941
AjuI GAANNNNNNNTTGG 2 cut(s) 2019, 2051
AleI CACNNNNGTG 2 cut(s) 591, 601
Alw21I GWGCWC 4 cut(s) 430, 598, 1173, 3078
Alw44I GTGCAC 2 cut(s) 594, 3074
AlwI GGATC 5 cut(s) 766, 916, 1639, 2090, 3009
AlwNI CAGNNNCTG 2 cut(s) 236, 1701
Ama87I CYCGRG 1 cut(s) 3277
Aor13HI TCCGGA 1 cut(s) 313
AoxI GGCC 5 cut(s) 28, 1042, 1443, 1514, 2177
ApaLI GTGCAC 2 cut(s) 594, 3074
ApeKI GCWGC 6 cut(s) 734, 1115, 1319, 1536, 2835, 3261
AspLEI GCGC 1 cut(s) 514
AspS9I GGNCC 5 cut(s) 29, 1740, 1914, 2177, 2783
AsuC2I CCSGG 1 cut(s) 2274
AsuHPI GGTGA 9 cut(s) 59, 284, 299, 592, 673, 1369, 1484, 2509, 2543
AvaI CYCGRG 1 cut(s) 3277
AvaII GGWCC 3 cut(s) 1740, 1914, 2783
BaeGI GKGCMC 4 cut(s) 598, 1078, 3078, 3086
BaeI ACNNNNGTAYC 2 cut(s) 2140, 2173
BalI TGGCCA 2 cut(s) 1445, 1516
BanII GRGCYC 1 cut(s) 618
BauI CACGAG 1 cut(s) 3077
BbrPI CACGTG 2 cut(s) 2174, 2672
BbsI GAAGAC 2 cut(s) 1260, 3261
Bbv12I GWGCWC 4 cut(s) 430, 598, 1173, 3078
BbvI GCAGC 6 cut(s) 746, 1127, 1306, 1523, 2822, 3248
BccI CCATC 5 cut(s) 300, 605, 1939, 2422, 2620
BceAI ACGGC 2 cut(s) 2736, 2774
BcgI CGANNNNNNTGC 2 cut(s) 197, 231
BciT130I CCWGG 4 cut(s) 380, 432, 1751, 2943
BclI TGATCA 5 cut(s) 157, 1059, 1281, 1359, 2931
BcnI CCSGG 1 cut(s) 2274
BfaI CTAG 7 cut(s) 738, 831, 978, 1127, 1956, 2351, 2393
BfmI CTRYAG 2 cut(s) 66, 3262
BfuAI ACCTGC 3 cut(s) 275, 1150, 3255
BglII AGATCT 2 cut(s) 1690, 2737
BisI GCNGC 7 cut(s) 735, 1116, 1320, 1537, 2836, 2839, 3262
BlpI GCTNAGC 1 cut(s) 1881
BlsI GCNGC 7 cut(s) 736, 1117, 1321, 1538, 2837, 2840, 3263
Bme1390I CCNGG 5 cut(s) 380, 432, 1751, 2274, 2943
Bme18I GGWCC 3 cut(s) 1740, 1914, 2783
BmeT110I CYCGRG 1 cut(s) 3277
BmgBI CACGTC 1 cut(s) 1166
BmgT120I GGNCC 5 cut(s) 29, 1740, 1914, 2177, 2783
BmiI GGNNCC 2 cut(s) 1915, 2448
BmrFI CCNGG 5 cut(s) 380, 432, 1751, 2274, 2943
BmrI ACTGGG 2 cut(s) 26, 1043
BmsI GCATC 5 cut(s) 27, 654, 842, 1306, 1466
BmuI ACTGGG 2 cut(s) 26, 1043
BoxI GACNNNNGTC 1 cut(s) 2386
BpiI GAAGAC 2 cut(s) 1260, 3261
BpmI CTGGAG 5 cut(s) 300, 453, 536, 1733, 2663
Bpu1102I GCTNAGC 1 cut(s) 1881
BpuEI CTTGAG 1 cut(s) 161
BpuMI CCSGG 1 cut(s) 2274
BsaAI YACGTR 2 cut(s) 2174, 2672
BsaI GGTCTC 1 cut(s) 2553
BsaJI CCNNGG 5 cut(s) 492, 657, 903, 2365, 3176
BsaWI WCCGGW 1 cut(s) 313
BsaXI ACNNNNNCTCC 2 cut(s) 434, 464
Bsc4I CCNNNNNNNGG 3 cut(s) 1273, 2948, 3248
Bse118I RCCGGY 1 cut(s) 1773
Bse1I ACTGG 5 cut(s) 32, 1049, 1093, 2769, 3034
Bse3DI GCAATG 1 cut(s) 2934
BseAI TCCGGA 1 cut(s) 313
BseBI CCWGG 4 cut(s) 380, 432, 1751, 2943
BseDI CCNNGG 5 cut(s) 492, 657, 903, 2365, 3176
BseLI CCNNNNNNNGG 3 cut(s) 1273, 2948, 3248
BseMI GCAATG 1 cut(s) 2934
BseMII CTCAG 6 cut(s) 662, 734, 1708, 1823, 2369, 2755
BseNI ACTGG 5 cut(s) 32, 1049, 1093, 2769, 3034
BseSI GKGCMC 4 cut(s) 598, 1078, 3078, 3086
BseXI GCAGC 6 cut(s) 746, 1127, 1306, 1523, 2822, 3248
Bsh1236I CGCG 1 cut(s) 512
BshFI GGCC 5 cut(s) 30, 1044, 1445, 1516, 2179
BsiHKAI GWGCWC 4 cut(s) 430, 598, 1173, 3078
BsiHKCI CYCGRG 1 cut(s) 3277
BsiSI CCGG 3 cut(s) 314, 1774, 2274
BslFI GGGAC 1 cut(s) 812
BslI CCNNNNNNNGG 3 cut(s) 1273, 2948, 3248
BsmFI GGGAC 1 cut(s) 812
BsmI GAATGC 3 cut(s) 979, 1351, 1896
BsnI GGCC 5 cut(s) 30, 1044, 1445, 1516, 2179
Bso31I GGTCTC 1 cut(s) 2553
BsoBI CYCGRG 1 cut(s) 3277
Bsp1286I GDGCHC 7 cut(s) 430, 598, 618, 1078, 1173, 3078, 3086
Bsp13I TCCGGA 1 cut(s) 313
Bsp1720I GCTNAGC 1 cut(s) 1881
Bsp19I CCATGG 4 cut(s) 492, 903, 2365, 3176
BspACI CCGC 6 cut(s) 5, 855, 1738, 2675, 2781, 2838
BspANI GGCC 5 cut(s) 30, 1044, 1445, 1516, 2179
BspCNI CTCAG 6 cut(s) 661, 735, 1707, 1824, 2370, 2754
BspEI TCCGGA 1 cut(s) 313
BspFNI CGCG 1 cut(s) 512
BspHI TCATGA 2 cut(s) 504, 3190
BspLI GGNNCC 2 cut(s) 1915, 2448
BspMAI CTGCAG 1 cut(s) 3266
BspMI ACCTGC 3 cut(s) 275, 1150, 3255
BspPI GGATC 5 cut(s) 766, 916, 1639, 2090, 3009
BspQI GCTCTTC 2 cut(s) 1578, 3189
BspTNI GGTCTC 1 cut(s) 2553
BsrBI CCGCTC 1 cut(s) 2781
BsrDI GCAATG 1 cut(s) 2934
BsrFI RCCGGY 1 cut(s) 1773
BsrI ACTGG 5 cut(s) 32, 1049, 1093, 2769, 3034
BssAI RCCGGY 1 cut(s) 1773
BssECI CCNNGG 5 cut(s) 492, 657, 903, 2365, 3176
BssSI CACGAG 1 cut(s) 3077
BssT1I CCWWGG 4 cut(s) 492, 903, 2365, 3176
Bst2BI CACGAG 1 cut(s) 3077
Bst2UI CCWGG 4 cut(s) 380, 432, 1751, 2943
Bst6I CTCTTC 5 cut(s) 338, 381, 1578, 2861, 3189
BstBAI YACGTR 2 cut(s) 2174, 2672
BstC8I GCNNGC 3 cut(s) 696, 1590, 2407
BstDSI CCRYGG 5 cut(s) 492, 657, 903, 2365, 3176
BstENI CCTNNNNNAGG 1 cut(s) 1271
BstFNI CGCG 1 cut(s) 512
BstHHI GCGC 1 cut(s) 514
BstMWI GCNNNNNNNGC 4 cut(s) 242, 734, 1804, 1891
BstNI CCWGG 4 cut(s) 380, 432, 1751, 2943
BstNSI RCATGY 4 cut(s) 354, 1596, 1978, 3286
BstPAI GACNNNNGTC 1 cut(s) 2386
BstSCI CCNGG 5 cut(s) 378, 430, 1749, 2272, 2941
BstSFI CTRYAG 2 cut(s) 66, 3262
BstSLI GKGCMC 4 cut(s) 598, 1078, 3078, 3086
BstUI CGCG 1 cut(s) 512
BstV1I GCAGC 6 cut(s) 746, 1127, 1306, 1523, 2822, 3248
BstV2I GAAGAC 2 cut(s) 1260, 3261
BstX2I RGATCY 6 cut(s) 771, 921, 1644, 1690, 2737, 3001
BstYI RGATCY 6 cut(s) 771, 921, 1644, 1690, 2737, 3001
BsuRI GGCC 5 cut(s) 30, 1044, 1445, 1516, 2179
BtgI CCRYGG 5 cut(s) 492, 657, 903, 2365, 3176
BtrI CACGTC 1 cut(s) 1166
BtsIMutI CAGTG 5 cut(s) 39, 453, 1353, 2961, 3027
BveI ACCTGC 3 cut(s) 275, 1150, 3255
Cac8I GCNNGC 3 cut(s) 696, 1590, 2407
CaiI CAGNNNCTG 2 cut(s) 236, 1701
CciI TCATGA 2 cut(s) 504, 3190
CfoI GCGC 1 cut(s) 514
Cfr10I RCCGGY 1 cut(s) 1773
Cfr13I GGNCC 5 cut(s) 29, 1740, 1914, 2177, 2783
CsiI ACCWGGT 1 cut(s) 378
Csp6I GTAC 2 cut(s) 1344, 2320
CspCI CAANNNNNGTGG 2 cut(s) 1710, 1745
CviQI GTAC 2 cut(s) 1344, 2320
DraI TTTAAA 1 cut(s) 133
EaeI YGGCCR 2 cut(s) 1443, 1514
Eam1104I CTCTTC 5 cut(s) 338, 381, 1578, 2861, 3189
EarI CTCTTC 5 cut(s) 338, 381, 1578, 2861, 3189
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 4 cut(s) 492, 903, 2365, 3176
Eco24I GRGCYC 1 cut(s) 618
Eco31I GGTCTC 1 cut(s) 2553
Eco32I GATATC 3 cut(s) 793, 1264, 1944
Eco47I GGWCC 3 cut(s) 1740, 1914, 2783
Eco57I CTGAAG 6 cut(s) 477, 1602, 1829, 1881, 2369, 2582
Eco72I CACGTG 2 cut(s) 2174, 2672
Eco88I CYCGRG 1 cut(s) 3277
EcoNI CCTNNNNNAGG 1 cut(s) 1271
EcoRI GAATTC 4 cut(s) 21, 825, 1926, 2483
EcoRII CCWGG 4 cut(s) 378, 430, 1749, 2941
EcoRV GATATC 3 cut(s) 793, 1264, 1944
EcoT14I CCWWGG 4 cut(s) 492, 903, 2365, 3176
EcoT22I ATGCAT 1 cut(s) 1528
EcoT38I GRGCYC 1 cut(s) 618
ErhI CCWWGG 4 cut(s) 492, 903, 2365, 3176
FalI AAGNNNNNCTT 6 cut(s) 92, 124, 666, 698, 855, 887
FaqI GGGAC 1 cut(s) 812
FauNDI CATATG 2 cut(s) 1528, 2774
FbaI TGATCA 5 cut(s) 157, 1059, 1281, 1359, 2931
FblI GTMKAC 3 cut(s) 995, 2755, 2907
Fnu4HI GCNGC 7 cut(s) 735, 1116, 1320, 1537, 2836, 2839, 3262
FriOI GRGCYC 1 cut(s) 618
Fsp4HI GCNGC 7 cut(s) 735, 1116, 1320, 1537, 2836, 2839, 3262
FspBI CTAG 7 cut(s) 738, 831, 978, 1127, 1956, 2351, 2393
GlaI GCGC 1 cut(s) 513
GluI GCNGC 7 cut(s) 735, 1116, 1320, 1537, 2836, 2839, 3262
GsuI CTGGAG 5 cut(s) 300, 453, 536, 1733, 2663
HaeIII GGCC 5 cut(s) 30, 1044, 1445, 1516, 2179
HapII CCGG 3 cut(s) 314, 1774, 2274
HhaI GCGC 1 cut(s) 514
Hin6I GCGC 1 cut(s) 512
HinP1I GCGC 1 cut(s) 512
HincII GTYRAC 2 cut(s) 2086, 2830
HindII GTYRAC 2 cut(s) 2086, 2830
HindIII AAGCTT 3 cut(s) 1574, 2403, 3019
HpaII CCGG 3 cut(s) 314, 1774, 2274
HphI GGTGA 9 cut(s) 59, 284, 299, 592, 673, 1369, 1484, 2509, 2543
Hpy99I CGWCG 4 cut(s) 426, 842, 1125, 2820
HpyAV CCTTC 4 cut(s) 452, 1526, 1675, 2012
HpyCH4IV ACGT 5 cut(s) 588, 1165, 2173, 2671, 2818
HpyF10VI GCNNNNNNNGC 4 cut(s) 242, 734, 1804, 1891
HpySE526I ACGT 5 cut(s) 588, 1165, 2173, 2671, 2818
HspAI GCGC 1 cut(s) 512
Kpn2I TCCGGA 1 cut(s) 313
Ksp22I TGATCA 5 cut(s) 157, 1059, 1281, 1359, 2931
LguI GCTCTTC 2 cut(s) 1578, 3189
LmnI GCTCC 3 cut(s) 281, 2200, 2778
Lsp1109I GCAGC 6 cut(s) 746, 1127, 1306, 1523, 2822, 3248
LweI GCATC 5 cut(s) 27, 654, 842, 1306, 1466
MabI ACCWGGT 1 cut(s) 378
MaeI CTAG 7 cut(s) 738, 831, 978, 1127, 1956, 2351, 2393
MaeII ACGT 5 cut(s) 588, 1165, 2173, 2671, 2818
MaeIII GTNAC 8 cut(s) 83, 329, 406, 529, 705, 2293, 2419, 2588
MbiI CCGCTC 1 cut(s) 2781
MfeI CAATTG 3 cut(s) 2134, 2581, 2888
MflI RGATCY 6 cut(s) 771, 921, 1644, 1690, 2737, 3001
MhlI GDGCHC 7 cut(s) 430, 598, 618, 1078, 1173, 3078, 3086
MlsI TGGCCA 2 cut(s) 1445, 1516
MluNI TGGCCA 2 cut(s) 1445, 1516
MlyI GAGTC 6 cut(s) 55, 91, 510, 1001, 1741, 2903
MmeI TCCRAC 4 cut(s) 1077, 1404, 2017, 3119
Mox20I TGGCCA 2 cut(s) 1445, 1516
Mph1103I ATGCAT 1 cut(s) 1528
MroI TCCGGA 1 cut(s) 313
MscI TGGCCA 2 cut(s) 1445, 1516
MslI CAYNNNNRTG 3 cut(s) 591, 601, 2351
Msp20I TGGCCA 2 cut(s) 1445, 1516
MspA1I CMGCKG 2 cut(s) 1807, 2835
MspI CCGG 3 cut(s) 314, 1774, 2274
MspR9I CCNGG 5 cut(s) 380, 432, 1751, 2274, 2943
MunI CAATTG 3 cut(s) 2134, 2581, 2888
Mva1269I GAATGC 3 cut(s) 979, 1351, 1896
MvaI CCWGG 4 cut(s) 380, 432, 1751, 2943
MvnI CGCG 1 cut(s) 512
MwoI GCNNNNNNNGC 4 cut(s) 242, 734, 1804, 1891
NciI CCSGG 1 cut(s) 2274
NcoI CCATGG 4 cut(s) 492, 903, 2365, 3176
NdeI CATATG 2 cut(s) 1528, 2774
NlaIV GGNNCC 2 cut(s) 1915, 2448
NmuCI GTSAC 2 cut(s) 83, 529
NsiI ATGCAT 1 cut(s) 1528
NspI RCATGY 4 cut(s) 354, 1596, 1978, 3286
OliI CACNNNNGTG 2 cut(s) 591, 601
PaeR7I CTCGAG 1 cut(s) 3277
PagI TCATGA 2 cut(s) 504, 3190
PaqCI CACCTGC 1 cut(s) 275
PciSI GCTCTTC 2 cut(s) 1578, 3189
PctI GAATGC 3 cut(s) 979, 1351, 1896
PfeI GAWTC 6 cut(s) 167, 950, 1498, 2231, 2476, 2599
PkrI GCNGC 7 cut(s) 736, 1117, 1321, 1538, 2837, 2840, 3263
PleI GAGTC 6 cut(s) 55, 90, 509, 1000, 1740, 2903
PmaCI CACGTG 2 cut(s) 2174, 2672
PmlI CACGTG 2 cut(s) 2174, 2672
PpsI GAGTC 6 cut(s) 55, 90, 509, 1000, 1740, 2903
Ppu21I YACGTR 2 cut(s) 2174, 2672
PshAI GACNNNNGTC 1 cut(s) 2386
PsiI TTATAA 1 cut(s) 2223
Psp6I CCWGG 4 cut(s) 378, 430, 1749, 2941
PspCI CACGTG 2 cut(s) 2174, 2672
PspGI CCWGG 4 cut(s) 378, 430, 1749, 2941
PspN4I GGNNCC 2 cut(s) 1915, 2448
PspPI GGNCC 5 cut(s) 29, 1740, 1914, 2177, 2783
PstI CTGCAG 1 cut(s) 3266
PstNI CAGNNNCTG 2 cut(s) 236, 1701
PsuI RGATCY 6 cut(s) 771, 921, 1644, 1690, 2737, 3001
PvuII CAGCTG 2 cut(s) 1807, 2835
RsaI GTAC 2 cut(s) 1345, 2321
RsaNI GTAC 2 cut(s) 1344, 2320
RseI CAYNNNNRTG 3 cut(s) 591, 601, 2351
SapI GCTCTTC 2 cut(s) 1578, 3189
SatI GCNGC 7 cut(s) 735, 1116, 1320, 1537, 2836, 2839, 3262
Sau96I GGNCC 5 cut(s) 29, 1740, 1914, 2177, 2783
SchI GAGTC 6 cut(s) 55, 91, 510, 1001, 1741, 2903
ScrFI CCNGG 5 cut(s) 380, 432, 1751, 2274, 2943
SduI GDGCHC 7 cut(s) 430, 598, 618, 1078, 1173, 3078, 3086
SexAI ACCWGGT 1 cut(s) 378
SfaNI GCATC 5 cut(s) 27, 654, 842, 1306, 1466
SfcI CTRYAG 2 cut(s) 66, 3262
Sfr274I CTCGAG 1 cut(s) 3277
SinI GGWCC 3 cut(s) 1740, 1914, 2783
SlaI CTCGAG 1 cut(s) 3277
SmiMI CAYNNNNRTG 3 cut(s) 591, 601, 2351
SmlI CTYRAG 2 cut(s) 140, 3277
SmoI CTYRAG 2 cut(s) 140, 3277
SsiI CCGC 6 cut(s) 5, 855, 1738, 2675, 2781, 2838
SspI AATATT 2 cut(s) 2074, 2218
SspMI CTAG 7 cut(s) 738, 831, 978, 1127, 1956, 2351, 2393
StyD4I CCNGG 5 cut(s) 378, 430, 1749, 2272, 2941
StyI CCWWGG 4 cut(s) 492, 903, 2365, 3176
TaiI ACGT 5 cut(s) 591, 1168, 2176, 2674, 2821
TaqI TCGA 7 cut(s) 217, 421, 990, 1120, 2815, 3143, 3278
TaqII GACCGA 1 cut(s) 2679
TatI WGTACW 1 cut(s) 1343
TauI GCSGC 1 cut(s) 2841
TfiI GAWTC 6 cut(s) 167, 950, 1498, 2231, 2476, 2599
TscAI CASTG 5 cut(s) 39, 460, 1353, 2961, 3034
TseFI GTSAC 2 cut(s) 83, 529
TseI GCWGC 6 cut(s) 734, 1115, 1319, 1536, 2835, 3261
Tsp45I GTSAC 2 cut(s) 83, 529
TspGWI ACGGA 5 cut(s) 1176, 1655, 3042, 3054, 3128
TspRI CASTG 5 cut(s) 39, 460, 1353, 2961, 3034
VneI GTGCAC 2 cut(s) 594, 3074
VpaK11BI GGWCC 3 cut(s) 1740, 1914, 2783
XagI CCTNNNNNAGG 1 cut(s) 1271
XceI RCATGY 4 cut(s) 354, 1596, 1978, 3286
XcmI CCANNNNNNNNNTGG 3 cut(s) 490, 842, 1387
XhoI CTCGAG 1 cut(s) 3277
XmiI GTMKAC 3 cut(s) 995, 2755, 2907
XspI CTAG 7 cut(s) 738, 831, 978, 1127, 1956, 2351, 2393
Zsp2I ATGCAT 1 cut(s) 1528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.