MD11G1020500.v1.1

disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000085
Physical Location & Seq
Reverse (-)
1779043 .. 1783097
4055 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1020500.v1.1.491

Sequence Viewer

Length: 2985 bp
ATGAATGGAGACGAAGTTAGGTCCGAGGTTCAAAAATGGCTCAAGGATGCTGACAAGGCCATAATCGATGCGAAAAGACTGAATAATGAAGCCGGAGAAAACAAAACATGCCTTGGAGGCTGCTGCCCGAATTTGAAATGGCGTTACACTTTAAGCAAGAGAGCAGTTAATGAGACAGAAGAATTGAACAAGATTAAAGAAGAGAAAAGGTTTGAAACAGTTTCGCTTCAAGTTCGCCGACCCGTTGAGTTTGAGTCCACCATGTCAACAGGAGATTTCGAAGCATTTGAAGCAACAAGGCAAGCCATGGATGGGGTCATGAAGGCGCTGAAAGACGATAACATCACTGTCATCGGGGTTCATGGAATGGGAGGCATAGGCAAGACAACCATGGTGAAACATGTTGGTGTGCAAGCGTGTAAAGACAAGCTCTTTGATCATGTTATTATGGCTGTCATTTCCCAAAACCCAAACCTGGTAAAGATTCAACAACAGCTTGCTGAAATGCTCGCCTTGAATTTGAATGAGCAGACAGAAATAGCCAGAGCAGCTAGATTGAAGGAGAGAATAATGAGAGGTAAAAAGATCCTTATAATTTTAGATGACATTTGGAGGACAATAGATTTGTCTCGCATAGGGATCCCCGATCATTGCGAGCTCCAAAACTGCAACTCCAAAGTCCTACTCACCACAAGGATATGGAATGTATGTCATGCCATGAAGAGCCAAGAAAAGATCCATCTCGATATCCTTTCAGAAGAAGATTCTTGGGCCTTGTTTGTGAAGAAGGCAAACAGGTCTTTTGAATCAACCAATTTATGTGACATAGCAAGGAAGGTAGCCAGAGAATGTGGTGGTCTCCCAATTGCTTTGATTGCAGTTGCAAGAGCACTCGGAGATAAGGACTTGGAAGAATGGAGAGAAGCAGCTCGACGACTAGAGATGTCCCAAACTACCAACCTTGATGACGAGGAGGATGTGTTCAGATGTATAAAGCTGAGCTACGATTACTTGAAAGGCAATGACGCGAAGTCATTTTTCTTACTTTGCTGCTTATTCCCGGAGGACTCTGATATCCAAATCGAAGACTTGCTGAAGTATGGTTTTGGGAAAGGATTGTTTCGAGATGCCAATTCAATGCAAGATGCCAGAAGCACAGCACATTTGGTGGCCAAGTACCTTAAAGCTTGTAGCTTGGTTTTGGATGGTGAACAAGATGGATGTGTAAGAATGCATGATGTGATTCGGGACATGGCAATGCGAATTGCATCATCTGGGGATGGCCAAGGATTTTTCGTAAAAGCTGGCTGCCACTTAAAGGATTGGCCAACGAATTCACTTGAAGACTACTCTGCAATCTCCCTAATGGGGAATGAAATTTGCAATCTTCCTGACGAGTTGGTATGTCCAAAACTCCAGATTTTATTACTACAGTCAAATTTTGAAGTACAGGAGATCCCAGAAGATTTTTTCAAAAGCCCAAATGCATTAAGGGTATTCGACATAAGTAGTACCGAAATTTCTTCATTACCATCATCATTTAATCTCTTAACCAAGCTCCAAACGCTGCATCTAGATCGTTGTCAGTCAATAACGGATGTATCAATTCTCGGAGAACTGAAAAAACTGGAGGTTCTTAGTTTGAGAGAATCTGGTATTGAGGAATTGCCGGAAGAAATTGGACATTTGACCAATTTAAGGATGTTGGATCTCACTATGAGTACCTACATTGCAACAATTCCATCCAAAGTGATATCGCAGCTGTCCAAATTAGAAGAACTATACATGCAACTGAGTTTCGCTGATTGGGGCAAGAAAGTTGAAGGAGAAGATGACAAAACTAATGCTAGTTTTGATGAGCTAATTAGCTTGTCTTCCTTAAACATTTTGAAGGTTGACATATCTGATGCAGAATGCCTTCCGAAAAATGTAGGGTTCCTTCGAAATTGGGTAAATTTCGATATATGTATCAGCAGAGACCCAGTTCATCGGTCCATGAATGTCCACTTATCAAAGAATATTGTTACTCCATATTCAAGAGCCTTGTTTCTGGACACATCAATCAATACACTGCCGGATTGGTTTAACACCACAGTTACGGAGAGAGTGGAGAAAATAATCTACTTGGAGCCTAGAAGTTTAACTGACATTCTTGTGGAGTATGATCATGGGAAGTTACATGGACTCAAGTTTTTGCATCTCCAAGAGTGCCGTGAGATAGTAACTTTGATAAACACAGTAACCAGGATTCCAAATAAGCCTGTGCTCGAGAGCTTGGAAGAGTTGCATGTCTTCTGCTGGGACTGCTTGCAACAGTTATGTATTGGTGAATTACTGCCGGGGTCGCTTGGAAATCTCAAGTTCTTGGAGGTGGAGCAGTGTGAAGGTGTGGCGGAGGCACTTGTTCCATCTAATCTGTTGCAGAGACTACAAAATCTTGAAGTACTTATTGTATGTGCCACTGGTATCGATTATATATTCAAATCTCAAGGGCTTGAACAAGGAGAAACGGTCTTGACAAAATTGGGAGAGATGAAACTGGAGAATCTACCGGAACTGACAAACATATGGAACGGTCCTTCTCAGCCTGACATGTTTCATAATCTTAAATCTCTGACGATTTCCAAGTGCAAGAAATTGAAAACTCTCTTCACATTCGATGTAGCTCAATGTCTGCGGCAATTGGAAGAGCTTTGGGTGGAGGAATGTCATAGCTTGGACAAACTTTTCGGCCTAAGCGAGGGATTTATAGTGCAGGATTACGAGATCATTTTTCGACAACTAAAGAACTTAGCATTGCAAAGTCTTTCATCGCTGACAAGCGTCTCCGCTGGAAGTGCTAGACTTTTGTTCCCTTCACTGGAATACTTGCATGTGCTTGACTGCCCCCGGTTTTTAACCTCTACTTCTGACTTCCACAGCAAGATGCAAGTCCAACTAAACAATGAGCAACATTTCCTCTTCCTAAGGAAAAGAGGTAATTAA

Protein Analysis

995

Amino Acids

112.68

Weight (kDa)

5.34

Isoelectric Point (pI)

44.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 101 - 266 1e-32 NB-ARC domain
LRR_8 PF13855 470 - 529 1.2e-08 Leucine rich repeat
LRR_14 PF23598 476 - 549 3e-07 Leucine-rich repeat region
LRR_14 PF23598 537 - 699 4.5e-11 Leucine-rich repeat region
LRR_RPS2 PF23247 770 - 903 8.3e-29 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 864 - 965 1.6e-15 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 593
AccII CGCG 1 cut(s) 1028
AciI CCGC 3 cut(s) 2393, 2677, 2829
AclWI GGATC 6 cut(s) 580, 634, 647, 730, 1450, 1716
AcoI YGGCCR 3 cut(s) 1170, 1282, 1325
AcsI RAATTY 7 cut(s) 130, 517, 1333, 1377, 1438, 1518, 1954
AcuI CTGAAG 1 cut(s) 1115
AfaI GTAC 5 cut(s) 1178, 1449, 1513, 1723, 2445
AfiI CCNNNNNNNGG 7 cut(s) 312, 475, 1318, 1368, 1698, 2740, 2860
AflIII ACRYGT 2 cut(s) 400, 2592
AhdI GACNNNNNGTC 1 cut(s) 1030
AjnI CCWGG 2 cut(s) 474, 2243
AloI GAACNNNNNNTCC 2 cut(s) 965, 997
Alw21I GWGCWC 3 cut(s) 660, 892, 2268
Alw26I GTCTC 7 cut(s) 3, 167, 633, 863, 1971, 2419, 2830
AlwI GGATC 6 cut(s) 580, 634, 647, 730, 1450, 1716
Ama87I CYCGRG 1 cut(s) 2267
AoxI GGCC 6 cut(s) 57, 771, 1170, 1282, 1325, 2731
ApeKI GCWGC 8 cut(s) 120, 123, 548, 926, 1050, 1308, 1567, 1759
ApoI RAATTY 7 cut(s) 130, 517, 1333, 1377, 1438, 1518, 1954
ArsI GACNNNNNNTTYG 6 cut(s) 416, 448, 607, 639, 2711, 2743
Asp700I GAANNNNTTC 1 cut(s) 1917
AspLEI GCGC 1 cut(s) 328
AspS9I GGNCC 4 cut(s) 21, 771, 1992, 2576
AsuC2I CCSGG 3 cut(s) 1061, 2340, 2890
AsuHPI GGTGA 4 cut(s) 406, 679, 1220, 2339
AsuII TTCGAA 2 cut(s) 279, 1942
AvaI CYCGRG 1 cut(s) 2267
AvaII GGWCC 3 cut(s) 21, 1992, 2576
AxyI CCTNAGG 1 cut(s) 2966
BaeI ACNNNNGTAYC 2 cut(s) 1584, 1617
BalI TGGCCA 3 cut(s) 1172, 1284, 1327
BamHI GGATCC 1 cut(s) 639
BanII GRGCYC 1 cut(s) 660
BarI GAAGNNNNNNTAC 2 cut(s) 1923, 1955
BbsI GAAGAC 4 cut(s) 1092, 1350, 1866, 2284
Bbv12I GWGCWC 3 cut(s) 660, 892, 2268
BbvI GCAGC 8 cut(s) 107, 110, 560, 938, 1037, 1295, 1554, 1771
BccI CCATC 8 cut(s) 305, 747, 1199, 1211, 1274, 1540, 1750, 2416
BceAI ACGGC 1 cut(s) 2196
BcgI CGANNNNNNTGC 2 cut(s) 1559, 1593
BciT130I CCWGG 2 cut(s) 476, 2245
BclI TGATCA 2 cut(s) 436, 2164
BcnI CCSGG 3 cut(s) 1061, 2340, 2890
BcoDI GTCTC 7 cut(s) 3, 167, 633, 863, 1971, 2419, 2830
BfaI CTAG 6 cut(s) 552, 938, 1574, 1848, 2133, 2841
BfmI CTRYAG 1 cut(s) 1430
BfoI RGCGCY 1 cut(s) 329
BglI GCCNNNNNGGC 1 cut(s) 117
BisI GCNGC 9 cut(s) 121, 124, 549, 927, 1051, 1309, 1568, 1760, 2678
BlpI GCTNAGC 1 cut(s) 998
BlsI GCNGC 9 cut(s) 122, 125, 550, 928, 1052, 1310, 1569, 1761, 2679
BmcAI AGTACT 1 cut(s) 2445
Bme1390I CCNGG 5 cut(s) 476, 1061, 2245, 2340, 2890
Bme18I GGWCC 3 cut(s) 21, 1992, 2576
BmeRI GACNNNNNGTC 1 cut(s) 1030
BmeT110I CYCGRG 1 cut(s) 2267
BmgT120I GGNCC 4 cut(s) 21, 771, 1992, 2576
BmiI GGNNCC 3 cut(s) 641, 1937, 2130
BmrFI CCNGG 5 cut(s) 476, 1061, 2245, 2340, 2890
BmrI ACTGGG 1 cut(s) 1976
BmsI GCATC 9 cut(s) 37, 58, 1117, 1135, 1277, 1579, 1897, 2206, 2916
BmuI ACTGGG 1 cut(s) 1976
BoxI GACNNNNGTC 1 cut(s) 2822
BpiI GAAGAC 4 cut(s) 1092, 1350, 1866, 2284
BpmI CTGGAG 3 cut(s) 1400, 1649, 2561
Bpu10I CCTNAGC 1 cut(s) 2735
Bpu1102I GCTNAGC 1 cut(s) 998
Bpu14I TTCGAA 2 cut(s) 279, 1942
BpuEI CTTGAG 4 cut(s) 26, 2171, 2342, 2472
BpuMI CCSGG 3 cut(s) 1061, 2340, 2890
Bsa29I ATCGAT 2 cut(s) 66, 2469
BsaI GGTCTC 2 cut(s) 863, 1971
BsaJI CCNNGG 7 cut(s) 24, 112, 306, 390, 1285, 2339, 2888
BsaWI WCCGGW 1 cut(s) 2551
BsaXI ACNNNNNCTCC 7 cut(s) 30, 656, 686, 965, 995, 2496, 2526
Bsc4I CCNNNNNNNGG 7 cut(s) 312, 475, 1318, 1368, 1698, 2740, 2860
Bse1I ACTGG 5 cut(s) 1632, 1982, 2467, 2544, 2865
Bse21I CCTNAGG 1 cut(s) 2966
Bse3DI GCAATG 5 cut(s) 649, 1027, 1263, 1728, 2795
BseBI CCWGG 2 cut(s) 476, 2245
BseCI ATCGAT 2 cut(s) 66, 2469
BseDI CCNNGG 7 cut(s) 24, 112, 306, 390, 1285, 2339, 2888
BseGI GGATG 9 cut(s) 52, 316, 982, 1210, 1226, 1285, 1603, 1707, 1742
BseLI CCNNNNNNNGG 7 cut(s) 312, 475, 1318, 1368, 1698, 2740, 2860
BseMI GCAATG 5 cut(s) 649, 1027, 1263, 1728, 2795
BseMII CTCAG 3 cut(s) 989, 1784, 2597
BseNI ACTGG 5 cut(s) 1632, 1982, 2467, 2544, 2865
BseRI GAGGAG 1 cut(s) 986
BseXI GCAGC 8 cut(s) 107, 110, 560, 938, 1037, 1295, 1554, 1771
BseYI CCCAGC 1 cut(s) 2298
BsgI GTGCAG 1 cut(s) 2774
Bsh1236I CGCG 1 cut(s) 1028
BshFI GGCC 6 cut(s) 59, 773, 1172, 1284, 1327, 2733
BshVI ATCGAT 2 cut(s) 66, 2469
BsiHKAI GWGCWC 3 cut(s) 660, 892, 2268
BsiHKCI CYCGRG 1 cut(s) 2267
BsiSI CCGG 7 cut(s) 93, 1061, 1670, 2075, 2339, 2552, 2890
BslFI GGGAC 3 cut(s) 931, 1262, 2315
BslI CCNNNNNNNGG 7 cut(s) 312, 475, 1318, 1368, 1698, 2740, 2860
BsmAI GTCTC 7 cut(s) 3, 167, 633, 863, 1971, 2419, 2830
BsmBI CGTCTC 2 cut(s) 3, 2830
BsmFI GGGAC 3 cut(s) 931, 1262, 2315
BsmI GAATGC 2 cut(s) 1236, 1919
BsnI GGCC 6 cut(s) 59, 773, 1172, 1284, 1327, 2733
Bso31I GGTCTC 2 cut(s) 863, 1971
BsoBI CYCGRG 1 cut(s) 2267
Bsp119I TTCGAA 2 cut(s) 279, 1942
Bsp1286I GDGCHC 3 cut(s) 660, 892, 2268
Bsp1720I GCTNAGC 1 cut(s) 998
Bsp19I CCATGG 2 cut(s) 306, 390
BspACI CCGC 3 cut(s) 2393, 2677, 2829
BspANI GGCC 6 cut(s) 59, 773, 1172, 1284, 1327, 2733
BspCNI CTCAG 3 cut(s) 990, 1785, 2596
BspDI ATCGAT 2 cut(s) 66, 2469
BspFNI CGCG 1 cut(s) 1028
BspHI TCATGA 1 cut(s) 318
BspLI GGNNCC 3 cut(s) 641, 1937, 2130
BspPI GGATC 6 cut(s) 580, 634, 647, 730, 1450, 1716
BspQI GCTCTTC 2 cut(s) 716, 2682
BspT104I TTCGAA 2 cut(s) 279, 1942
BspTNI GGTCTC 2 cut(s) 863, 1971
BsrDI GCAATG 5 cut(s) 649, 1027, 1263, 1728, 2795
BsrI ACTGG 5 cut(s) 1632, 1982, 2467, 2544, 2865
BssECI CCNNGG 7 cut(s) 24, 112, 306, 390, 1285, 2339, 2888
BssT1I CCWWGG 4 cut(s) 112, 306, 390, 1285
Bst2UI CCWGG 2 cut(s) 476, 2245
Bst4CI ACNGT 8 cut(s) 220, 349, 1434, 2095, 2239, 2316, 2512, 2576
Bst6I CTCTTC 6 cut(s) 195, 716, 2274, 2654, 2682, 2966
BstBI TTCGAA 2 cut(s) 279, 1942
BstC8I GCNNGC 7 cut(s) 303, 414, 498, 510, 656, 1306, 2309
BstDEI CTNAG 7 cut(s) 998, 1637, 1793, 2583, 2735, 2791, 2966
BstDSI CCRYGG 2 cut(s) 306, 390
BstENI CCTNNNNNAGG 1 cut(s) 2738
BstF5I GGATG 9 cut(s) 52, 316, 982, 1210, 1226, 1285, 1603, 1707, 1742
BstFNI CGCG 1 cut(s) 1028
BstH2I RGCGCY 1 cut(s) 329
BstHHI GCGC 1 cut(s) 328
BstMAI GTCTC 7 cut(s) 3, 167, 633, 863, 1971, 2419, 2830
BstMWI GCNNNNNNNGC 9 cut(s) 56, 117, 290, 548, 875, 1564, 2304, 2344, 2837
BstNI CCWGG 2 cut(s) 476, 2245
BstNSI RCATGY 6 cut(s) 111, 404, 1789, 2291, 2596, 2876
BstPAI GACNNNNGTC 1 cut(s) 2822
BstSCI CCNGG 5 cut(s) 474, 1059, 2243, 2338, 2888
BstSFI CTRYAG 1 cut(s) 1430
BstUI CGCG 1 cut(s) 1028
BstV1I GCAGC 8 cut(s) 107, 110, 560, 938, 1037, 1295, 1554, 1771
BstV2I GAAGAC 4 cut(s) 1092, 1350, 1866, 2284
BstX2I RGATCY 5 cut(s) 585, 639, 735, 1455, 1708
BstYI RGATCY 5 cut(s) 585, 639, 735, 1455, 1708
Bsu15I ATCGAT 2 cut(s) 66, 2469
Bsu36I CCTNAGG 1 cut(s) 2966
BsuRI GGCC 6 cut(s) 59, 773, 1172, 1284, 1327, 2733
BsuTUI ATCGAT 2 cut(s) 66, 2469
BtgI CCRYGG 2 cut(s) 306, 390
BtgZI GCGATG 1 cut(s) 2796
BtsCI GGATG 9 cut(s) 52, 316, 982, 1210, 1226, 1285, 1603, 1707, 1742
BtsI GCAGTG 2 cut(s) 2069, 2384
BtsIMutI CAGTG 5 cut(s) 345, 2069, 2384, 2460, 2858
Cac8I GCNNGC 7 cut(s) 303, 414, 498, 510, 656, 1306, 2309
CciI TCATGA 1 cut(s) 318
CfoI GCGC 1 cut(s) 328
Cfr13I GGNCC 4 cut(s) 21, 771, 1992, 2576
ClaI ATCGAT 2 cut(s) 66, 2469
CseI GACGC 2 cut(s) 1034, 2812
CsiI ACCWGGT 1 cut(s) 474
Csp6I GTAC 5 cut(s) 1177, 1448, 1512, 1722, 2444
CviQI GTAC 5 cut(s) 1177, 1448, 1512, 1722, 2444
DdeI CTNAG 7 cut(s) 998, 1637, 1793, 2583, 2735, 2791, 2966
DriI GACNNNNNGTC 1 cut(s) 1030
EaeI YGGCCR 3 cut(s) 1170, 1282, 1325
Eam1104I CTCTTC 6 cut(s) 195, 716, 2274, 2654, 2682, 2966
Eam1105I GACNNNNNGTC 1 cut(s) 1030
EarI CTCTTC 6 cut(s) 195, 716, 2274, 2654, 2682, 2966
EciI GGCGGA 1 cut(s) 2408
Ecl136II GAGCTC 1 cut(s) 658
Eco130I CCWWGG 4 cut(s) 112, 306, 390, 1285
Eco24I GRGCYC 1 cut(s) 660
Eco31I GGTCTC 2 cut(s) 863, 1971
Eco32I GATATC 3 cut(s) 748, 1075, 1755
Eco47I GGWCC 3 cut(s) 21, 1992, 2576
Eco53kI GAGCTC 1 cut(s) 658
Eco57I CTGAAG 1 cut(s) 1115
Eco81I CCTNAGG 1 cut(s) 2966
Eco88I CYCGRG 1 cut(s) 2267
EcoICRI GAGCTC 1 cut(s) 658
EcoNI CCTNNNNNAGG 1 cut(s) 2738
EcoRI GAATTC 1 cut(s) 1333
EcoRII CCWGG 2 cut(s) 474, 2243
EcoRV GATATC 3 cut(s) 748, 1075, 1755
EcoT14I CCWWGG 4 cut(s) 112, 306, 390, 1285
EcoT22I ATGCAT 2 cut(s) 1236, 1489
EcoT38I GRGCYC 1 cut(s) 660
ErhI CCWWGG 4 cut(s) 112, 306, 390, 1285
Esp3I CGTCTC 2 cut(s) 3, 2830
FaqI GGGAC 3 cut(s) 931, 1262, 2315
FauNDI CATATG 1 cut(s) 2567
FbaI TGATCA 2 cut(s) 436, 2164
Fnu4HI GCNGC 9 cut(s) 121, 124, 549, 927, 1051, 1309, 1568, 1760, 2678
FokI GGATG 9 cut(s) 59, 323, 989, 1217, 1233, 1292, 1610, 1714, 1729
FriOI GRGCYC 1 cut(s) 660
Fsp4HI GCNGC 9 cut(s) 121, 124, 549, 927, 1051, 1309, 1568, 1760, 2678
FspBI CTAG 6 cut(s) 552, 938, 1574, 1848, 2133, 2841
GlaI GCGC 1 cut(s) 327
GluI GCNGC 9 cut(s) 121, 124, 549, 927, 1051, 1309, 1568, 1760, 2678
GsaI CCCAGC 1 cut(s) 2302
GsuI CTGGAG 3 cut(s) 1400, 1649, 2561
HaeII RGCGCY 1 cut(s) 329
HaeIII GGCC 6 cut(s) 59, 773, 1172, 1284, 1327, 2733
HapII CCGG 7 cut(s) 93, 1061, 1670, 2075, 2339, 2552, 2890
HgaI GACGC 2 cut(s) 1034, 2812
HhaI GCGC 1 cut(s) 328
Hin6I GCGC 1 cut(s) 326
HinP1I GCGC 1 cut(s) 326
HincII GTYRAC 2 cut(s) 267, 1897
HindII GTYRAC 2 cut(s) 267, 1897
HindIII AAGCTT 1 cut(s) 1185
HpaII CCGG 7 cut(s) 93, 1061, 1670, 2075, 2339, 2552, 2890
HphI GGTGA 4 cut(s) 406, 679, 1220, 2339
Hpy166II GTNNAC 5 cut(s) 258, 267, 1211, 1897, 2005
Hpy8I GTNNAC 5 cut(s) 258, 267, 1211, 1897, 2005
Hpy99I CGWCG 1 cut(s) 936
HpyCH4III ACNGT 8 cut(s) 220, 349, 1434, 2095, 2239, 2316, 2512, 2576
HpyF10VI GCNNNNNNNGC 9 cut(s) 56, 117, 290, 548, 875, 1564, 2304, 2344, 2837
HpyF3I CTNAG 7 cut(s) 998, 1637, 1793, 2583, 2735, 2791, 2966
HspAI GCGC 1 cut(s) 326
Ksp22I TGATCA 2 cut(s) 436, 2164
LguI GCTCTTC 2 cut(s) 716, 2682
LmnI GCTCC 4 cut(s) 663, 1563, 2128, 2374
Lsp1109I GCAGC 8 cut(s) 107, 110, 560, 938, 1037, 1295, 1554, 1771
LweI GCATC 9 cut(s) 37, 58, 1117, 1135, 1277, 1579, 1897, 2206, 2916
MabI ACCWGGT 1 cut(s) 474
MaeI CTAG 6 cut(s) 552, 938, 1574, 1848, 2133, 2841
MaeIII GTNAC 7 cut(s) 143, 821, 2023, 2095, 2175, 2221, 2239
MfeI CAATTG 2 cut(s) 864, 2681
MflI RGATCY 5 cut(s) 585, 639, 735, 1455, 1708
MhlI GDGCHC 3 cut(s) 660, 892, 2268
MlsI TGGCCA 3 cut(s) 1172, 1284, 1327
MluNI TGGCCA 3 cut(s) 1172, 1284, 1327
MlyI GAGTC 3 cut(s) 263, 1061, 2178
MmeI TCCRAC 2 cut(s) 1686, 2959
Mox20I TGGCCA 3 cut(s) 1172, 1284, 1327
Mph1103I ATGCAT 2 cut(s) 1236, 1489
MroXI GAANNNNTTC 1 cut(s) 1917
MscI TGGCCA 3 cut(s) 1172, 1284, 1327
MslI CAYNNNNRTG 3 cut(s) 405, 1256, 2153
Msp20I TGGCCA 3 cut(s) 1172, 1284, 1327
MspA1I CMGCKG 2 cut(s) 1762, 2831
MspI CCGG 7 cut(s) 93, 1061, 1670, 2075, 2339, 2552, 2890
MspR9I CCNGG 5 cut(s) 476, 1061, 2245, 2340, 2890
MunI CAATTG 2 cut(s) 864, 2681
Mva1269I GAATGC 2 cut(s) 1236, 1919
MvaI CCWGG 2 cut(s) 476, 2245
MvnI CGCG 1 cut(s) 1028
MwoI GCNNNNNNNGC 9 cut(s) 56, 117, 290, 548, 875, 1564, 2304, 2344, 2837
NciI CCSGG 3 cut(s) 1061, 2340, 2890
NcoI CCATGG 2 cut(s) 306, 390
NdeI CATATG 1 cut(s) 2567
NlaIV GGNNCC 3 cut(s) 641, 1937, 2130
NmuCI GTSAC 1 cut(s) 821
NsiI ATGCAT 2 cut(s) 1236, 1489
NspI RCATGY 6 cut(s) 111, 404, 1789, 2291, 2596, 2876
NspV TTCGAA 2 cut(s) 279, 1942
PaeR7I CTCGAG 1 cut(s) 2267
PagI TCATGA 1 cut(s) 318
PciI ACATGT 2 cut(s) 400, 2592
PciSI GCTCTTC 2 cut(s) 716, 2682
PcsI WCGNNNNNNNCGW 1 cut(s) 2736
PctI GAATGC 2 cut(s) 1236, 1919
PdmI GAANNNNTTC 1 cut(s) 1917
PfeI GAWTC 7 cut(s) 484, 764, 806, 1243, 1649, 2248, 2545
PfoI TCCNGGA 1 cut(s) 1059
PkrI GCNGC 9 cut(s) 122, 125, 550, 928, 1052, 1310, 1569, 1761, 2679
PleI GAGTC 3 cut(s) 262, 1061, 2178
PpsI GAGTC 3 cut(s) 262, 1061, 2178
PscI ACATGT 2 cut(s) 400, 2592
PshAI GACNNNNGTC 1 cut(s) 2822
PsiI TTATAA 1 cut(s) 593
Psp124BI GAGCTC 1 cut(s) 660
Psp6I CCWGG 2 cut(s) 474, 2243
PspFI CCCAGC 1 cut(s) 2298
PspGI CCWGG 2 cut(s) 474, 2243
PspN4I GGNNCC 3 cut(s) 641, 1937, 2130
PspPI GGNCC 4 cut(s) 21, 771, 1992, 2576
PsuI RGATCY 5 cut(s) 585, 639, 735, 1455, 1708
PvuII CAGCTG 1 cut(s) 1762
RsaI GTAC 5 cut(s) 1178, 1449, 1513, 1723, 2445
RsaNI GTAC 5 cut(s) 1177, 1448, 1512, 1722, 2444
RseI CAYNNNNRTG 3 cut(s) 405, 1256, 2153
SacI GAGCTC 1 cut(s) 660
SapI GCTCTTC 2 cut(s) 716, 2682
SatI GCNGC 9 cut(s) 121, 124, 549, 927, 1051, 1309, 1568, 1760, 2678
Sau96I GGNCC 4 cut(s) 21, 771, 1992, 2576
ScaI AGTACT 1 cut(s) 2445
SchI GAGTC 3 cut(s) 263, 1061, 2178
ScrFI CCNGG 5 cut(s) 476, 1061, 2245, 2340, 2890
SduI GDGCHC 3 cut(s) 660, 892, 2268
SexAI ACCWGGT 1 cut(s) 474
SfaNI GCATC 9 cut(s) 37, 58, 1117, 1135, 1277, 1579, 1897, 2206, 2916
SfcI CTRYAG 1 cut(s) 1430
Sfr274I CTCGAG 1 cut(s) 2267
SfuI TTCGAA 2 cut(s) 279, 1942
SinI GGWCC 3 cut(s) 21, 1992, 2576
SlaI CTCGAG 1 cut(s) 2267
SmiMI CAYNNNNRTG 3 cut(s) 405, 1256, 2153
SmlI CTYRAG 5 cut(s) 41, 2186, 2267, 2357, 2487
SmoI CTYRAG 5 cut(s) 41, 2186, 2267, 2357, 2487
SsiI CCGC 3 cut(s) 2393, 2677, 2829
SspI AATATT 1 cut(s) 2020
SspMI CTAG 6 cut(s) 552, 938, 1574, 1848, 2133, 2841
SstI GAGCTC 1 cut(s) 660
StyD4I CCNGG 5 cut(s) 474, 1059, 2243, 2338, 2888
StyI CCWWGG 4 cut(s) 112, 306, 390, 1285
TaaI ACNGT 8 cut(s) 220, 349, 1434, 2095, 2239, 2316, 2512, 2576
TaqII GACCGA 1 cut(s) 1980
TatI WGTACW 2 cut(s) 1447, 2443
TauI GCSGC 1 cut(s) 2680
TfiI GAWTC 7 cut(s) 484, 764, 806, 1243, 1649, 2248, 2545
TscAI CASTG 5 cut(s) 352, 2076, 2384, 2467, 2865
TseFI GTSAC 1 cut(s) 821
TseI GCWGC 8 cut(s) 120, 123, 548, 926, 1050, 1308, 1567, 1759
Tsp45I GTSAC 1 cut(s) 821
TspGWI ACGGA 2 cut(s) 1610, 2114
TspRI CASTG 5 cut(s) 352, 2076, 2384, 2467, 2865
VpaK11BI GGWCC 3 cut(s) 21, 1992, 2576
XagI CCTNNNNNAGG 1 cut(s) 2738
XapI RAATTY 7 cut(s) 130, 517, 1333, 1377, 1438, 1518, 1954
XbaI TCTAGA 1 cut(s) 1573
XceI RCATGY 6 cut(s) 111, 404, 1789, 2291, 2596, 2876
XhoI CTCGAG 1 cut(s) 2267
XmnI GAANNNNTTC 1 cut(s) 1917
XspI CTAG 6 cut(s) 552, 938, 1574, 1848, 2133, 2841
ZrmI AGTACT 1 cut(s) 2445
Zsp2I ATGCAT 2 cut(s) 1236, 1489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.