Rw5G037770

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000085
Physical Location & Seq
Reverse (-)
66547772 .. 66554706
6935 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G037770.1

Sequence Viewer

Length: 3825 bp
ATGAAGGATGGTGCGGAACAATGCCAGAAGGACAGATTGTCCAACAAATCGCGGAATTTGTCTGCAGCAAACCTATTGAAATTAAGTCGACGGAAGATATTGAAACTCATGGAACTTTCTACAGAGTCGACAGAAGATTTTGAAGCAACAGGAGATGTCATGGACCGAACTTCTCCCCAGCCTGACCCAGCTCCTGTGTGGAAGAATGATGTGCTTTCGGGTTTCGGGATGGAAGACACTCGCAGGGGTTTTGTAGCCCATTCAGACCATGAATTGCCAAACACAGGACTAAGTAAAACATTCAAGGATGAAGACCAGCTTGAAAAAGGGGTAGCCCTCCCCTCTGCATCTCTTTCCTCAACTGAACCAAGTCCTCAACCAGCTCTTGGGCAGAAGAATGATGAGTTTTTGGGTTTCAGGGGAAACGACAATCATAGGGGTTTTGTCACCCATTCAAGTAATGAAATACAAAACACCAGGGTTACTAAGACATTCACCGATGAAGAACATCTTGAAAAAGGGATAGCTGCGTCTCCGCCTTCATCAGCTGAGCGAGATCCTCGGCCGAAGTATGATGTGTTTTTGAGTTACAGGGGGGAAGACACTCGTAGGGGTTTTATAGCCTATTTATACCGTGAATTGCAAAACACCAGAGTGATCAAAACTTTCAAGGATGACGTGCAACTAGAAAAAGGGACGGTTATTGCTTCAAATCTCCTAACGGCAATCAAAGAATCAAGGTTTGCGATCATTGTGTTGTCAAAAAATTATGCTTCTTCCTCATGGTGTTTGGATGAACTTGCAATGATTTTCCAGTGCATGAAAGATAAGAACACAATTTTCCCAATTTTTTATGAGGTGGAACCCACTGATGTACGATTTCAAAAGGAAGAGACAGGTTTTGGAGAAGCTTTCATTAAGCATGAAGCTACACAAGACATTGAGAAGATGAACCAGTGGAGAGCTGCTTTAAAAGGAGTGGCTGGAATCTCTGGGTGGCATTCAACCAACTATCCGACGGATATAGAACTAATTGAAGACATTGTGAAACATTTGTGGGAGAAAGTACTTGAAGCACCCACCGGAAATTTTGAAACATTTGAAGCGACAAGGCAAGCCATGGATGAGGTTATGAAGGTAATAAAAGATGATCAAGTCACTGTCCTTGGGGTGCATGGAGTGGCTGGCGTTGGAAAGACAACGATGGTCAAACATGTTAGTACAGAAGCCCAGAAAGGTGGGCTTTTTGATCATGTGATTATGGTTGTCGTATCCAAAGCCCCTGACTTGCAAGAAATTCAAGGCACATTGGCAGATTCGCTGGGCTTGGAGGAGGGGATCATGAGAGGAAACAGCATGCTTATAATCTTGGACGACATTTGGGAGAGTTTTGAACTCTCGAGCATTGGAATTCCCAATCACAATGAGCTTCAAAGGTGCAATTCTAAAGTTATACTCACCACAAGGAAAAGCAATATATGCCATTCTATGGGTTGCCATGCCCAGATCCCTCTCAATGTTCTATCGGAGGGAGATTCGTGGAAGTTATTCATGAAGACGTCGGGGAAGTCTTTTCATGAATCTACCGTTTCCTATGATGAAGCGAGAAAGATAGCTGGAGAATGCGGTGGTCTCCCAAAAGCAATAAAAGCAGCTGCAAGGTCACTTGGAGATAAATACATGGACGAATGGAATGAAGCAGTTGAAACAGCATTTGGAGATGACTGCTTGGACGAATGGAATGAAACAGCTCATCCTGTCAACTTCAAAGCTGAGGAAGACGTATTTCATTCATTGGCAACTGATGACGCCAAGTCATGCTTCTTGCTATGCTGCCTATTCCCAGAAAATTATGATATCCCAATTGAAGTGTTGCTCAGGTATGGGATTGGAATAGGTTTGTTTCAATATGATACAATGCAAGAAGCCAGAGATAGAGCACATTCTGTGGTCAAGTACCTCAAAGCTTCTAGATTGCTTTTGGACGGTACAAAGAAGGGATGCACAAGGATGACTGATGGCATTCGAAATCTGGCCATGTCCATTATGCGGTCCGAAGAAGGTGATGGGTTCTTAGTAAAAGCTGGTTGTGAACTAGAGGAGTGGCCAGAGGCAGGATTTTGGCAAGGAATTACTTTCCGGCTCCGCAAAATCTGGGATTGGCCAATGGATGCTCATGAAGGCTACTCAATAATCTCACTCATGAAAAACAAGCTTCATAAGCTTCCCGAATATTTGGTATGTTCAGAGCTCCAAATTTTATTGTTACAAAGTAATGCTGGTATACGTGACATCCCAGAAACCTTTCTTCAAAGTCCTAATGCGCTAAGGGTCTTTGATCTTAGCTTCACTAGCATTTCATCACTACCTTCATCATTCAGTTTCCTAACCAACCTTCAAGTTTTGAATTTGGATTTTTGCCATAATTTAAGTAACATATCCGTTCTGGGAAAACTTAAGAAACTTGAGATTCTTAGTATGAGAGAATTTCCTCTTAAGAAATTTCCAAAACAAATAGGAGATTTGACCAGTCTAAGGATGTTGGACGTCACTGGTAGAGATCTTGAAACAATCCCATCTAAAGTGATATCGAGGTTGTATAGATTGGAAGAACTGCACATGAAATTCCAGTTTGGGAAGTGGGGAAGTAAAATTGAGGATGCAGTTGAAGAAACCAATGCTGACTTTGATGAGTTGACTGGCTTGTTGGATTTAAACATTTTGGGAGTTTACATATCTGATGCTGCATGCTTGCCTAAAAGTGTTGAGTACAAGCCGAATTGGGTAGAATTTGATATATGCATCAGCAGAGATGCCCCTTGCGTGTCGCTCCCTCAACATGATGGTTTTTCAAGTTCCTTGACTCTTGACACAACAATCAATTGCTTACCGGATTGGTTTATCAGCGTTGTGACAGAGAAAGTGGAGAAGCTAGAGTACACAGATTGTGAAGACTTGAATGACATCGTTGGGCAATATGACCATGGCAGATTACTTGCACTGAAATATCTCTCTGTGAACGGGCACCATAAGAACTTGAAAGAGATAATCAACACAATGACACGGGTTTCAAATAAACCAGTATTTGAGAACTTAGAACAGTTGCATCTGTACCGCTTATATTCCCTCAAGGAGTTATGTGTTGGTGAGCTACCACCTGGGTCTCTACACAAGCTAAAGTTGTTGAAAGTGCAAACTTGTTGTAGCTTGGTGAATGCACTTTTACCATCGACATTGTTGCAGAGACTACAAAATCTGGAAGAAATCATTTGTGTAAATCTAGAAGGAATGAAATATGTATTTAGATCTGATGAAGGGCTTGATCCAGAAAATACTTTCTTGAGGAAATTGAGGGAAATGAGATTGTCTACTCTAGACAAACTAGTAAGCATATGTAATGGTCCTGCTCCGGATGCAATCTTCCATAATCTAAAAGTTTTGGCAATTTGCAAATGCAAGGAATTGAAAAATCTCTTCACAATAGGTGTAGCTGAATGTCTTTCTCAGTTGGAAGACCTTTCCGTACAGCAGTGTCATAGCTTGGACAGAGTCATTGAAGTAAGCAATAGCTTGAACAGCAAGATTAAACTTCCGGCGTTGAAGAAATTAGCTTTGATAGATCTTCCGGTGCTCACAAGGTTCTGCACTGAAAGTGTTACTATCGAAACTGAGTGCCCTTCATTGGAATACTTGTACGTGGAGAAGTGCCTCCAGTTTTCATATTTTGCTTACGACTTCAACAACAAGAACCAAGTCCACTTCAATGATGAACAACATTTGAGTTCTCTAGTGAAAAGGTGGGAGGAAGTGCATACTCGGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1274

Amino Acids

143.98

Weight (kDa)

5.4

Isoelectric Point (pI)

39.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 190 - 361 5.6e-47 TIR domain
TIR_2 PF13676 193 - 289 9.2e-14 TIR domain
NB-ARC PF00931 372 - 521 1.5e-24 NB-ARC domain
LRR_8 PF13855 750 - 808 9.8e-08 Leucine rich repeat
LRR_14 PF23598 779 - 875 1.8e-11 Leucine-rich repeat region
LRR_RPS2 PF23247 1032 - 1178 2.6e-24 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1136 - 1239 2.8e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1364
AatII GACGTC 2 cut(s) 1562, 2550
AccB1I GGYRCC 1 cut(s) 3024
AccB7I CCANNNNNTGG 2 cut(s) 386, 1489
AccI GTMKAC 4 cut(s) 88, 128, 2284, 3368
AccII CGCG 1 cut(s) 52
AccIII TCCGGA 1 cut(s) 3409
AciI CCGC 7 cut(s) 14, 52, 536, 1626, 2050, 2146, 3115
AclWI GGATC 4 cut(s) 551, 1346, 1501, 3317
AcoI YGGCCR 4 cut(s) 563, 2034, 2105, 2162
AcyI GRCGYC 3 cut(s) 1559, 1809, 2547
AfiI CCNNNNNNNGG 7 cut(s) 284, 386, 1489, 2049, 2114, 2535, 3682
AflII CTTAAG 2 cut(s) 2456, 2495
AflIII ACRYGT 1 cut(s) 1213
AhdI GACNNNNNGTC 2 cut(s) 37, 1813
AhlI ACTAGT 1 cut(s) 3382
AjiI CACGTC 1 cut(s) 679
AjnI CCWGG 2 cut(s) 476, 3157
AjuI GAANNNNNNNTTGG 4 cut(s) 1698, 1730, 1805, 1837
AleI CACNNNNGTG 1 cut(s) 653
Alw21I GWGCWC 3 cut(s) 1942, 2253, 3633
Alw26I GTCTC 5 cut(s) 537, 887, 1637, 3168, 3238
AlwI GGATC 4 cut(s) 551, 1346, 1501, 3317
AlwNI CAGNNNCTG 1 cut(s) 194
Ama87I CYCGRG 1 cut(s) 1399
Aor13HI TCCGGA 1 cut(s) 3409
AoxI GGCC 4 cut(s) 563, 2034, 2105, 2162
ApeKI GCWGC 7 cut(s) 65, 527, 965, 1652, 1655, 1833, 2744
Asp700I GAANNNNTTC 2 cut(s) 1547, 2304
AspLEI GCGC 1 cut(s) 2326
AspS9I GGNCC 3 cut(s) 163, 2052, 3401
AsuHPI GGTGA 6 cut(s) 439, 487, 1450, 2075, 3158, 3223
AsuII TTCGAA 1 cut(s) 2026
AvaI CYCGRG 1 cut(s) 1399
AvaII GGWCC 3 cut(s) 163, 2052, 3401
BaeGI GKGCMC 2 cut(s) 3027, 3677
BalI TGGCCA 3 cut(s) 2036, 2107, 2164
BanI GGYRCC 1 cut(s) 3024
BanII GRGCYC 1 cut(s) 2253
BarI GAAGNNNNNNTAC 2 cut(s) 2921, 2953
BbsI GAAGAC 8 cut(s) 240, 318, 606, 1044, 1562, 1785, 2958, 3519
Bbv12I GWGCWC 3 cut(s) 1942, 2253, 3633
BbvCI CCTCAGC 1 cut(s) 1773
BbvI GCAGC 7 cut(s) 77, 514, 952, 1642, 1664, 1820, 2731
BccI CCATC 8 cut(s) 2, 223, 1198, 2012, 2060, 2584, 2837, 3235
BceAI ACGGC 1 cut(s) 738
BcgI CGANNNNNNTGC 2 cut(s) 3220, 3254
BciT130I CCWGG 2 cut(s) 478, 3159
BciVI GTATCC 1 cut(s) 1282
BclI TGATCA 3 cut(s) 657, 1150, 1249
BcoDI GTCTC 5 cut(s) 537, 887, 1637, 3168, 3238
BcuI ACTAGT 1 cut(s) 3382
BfaI CTAG 9 cut(s) 686, 1971, 2096, 2352, 2933, 3281, 3374, 3383, 3788
BfmI CTRYAG 2 cut(s) 63, 120
BfrI CTTAAG 2 cut(s) 2456, 2495
BfuI GTATCC 1 cut(s) 1282
BglII AGATCT 3 cut(s) 2560, 3305, 3619
BisI GCNGC 7 cut(s) 66, 528, 966, 1653, 1656, 1834, 2745
BlpI GCTNAGC 1 cut(s) 549
BlsI GCNGC 7 cut(s) 67, 529, 967, 1654, 1657, 1835, 2746
BmcAI AGTACT 1 cut(s) 1068
Bme1390I CCNGG 2 cut(s) 478, 3159
Bme18I GGWCC 3 cut(s) 163, 2052, 3401
BmeRI GACNNNNNGTC 2 cut(s) 37, 1813
BmeT110I CYCGRG 1 cut(s) 1399
BmgBI CACGTC 1 cut(s) 679
BmgT120I GGNCC 3 cut(s) 163, 2052, 3401
BmiI GGNNCC 3 cut(s) 864, 2144, 3026
BmrFI CCNGG 2 cut(s) 478, 3159
BmsI GCATC 9 cut(s) 356, 1991, 2161, 2650, 2731, 2803, 2811, 3115, 3403
BpiI GAAGAC 8 cut(s) 240, 318, 606, 1044, 1562, 1785, 2958, 3519
BpmI CTGGAG 2 cut(s) 1638, 3695
Bpu10I CCTNAGC 3 cut(s) 1773, 1877, 2327
Bpu1102I GCTNAGC 1 cut(s) 549
Bpu14I TTCGAA 1 cut(s) 2026
BpuEI CTTGAG 3 cut(s) 2486, 3113, 3361
BsaAI YACGTR 2 cut(s) 2288, 3697
BsaHI GRCGYC 3 cut(s) 1559, 1809, 2547
BsaI GGTCTC 2 cut(s) 1637, 3168
BsaJI CCNNGG 6 cut(s) 477, 560, 1119, 1165, 2983, 3158
BsaWI WCCGGW 4 cut(s) 1082, 2890, 3409, 3625
Bsc4I CCNNNNNNNGG 7 cut(s) 284, 386, 1489, 2049, 2114, 2535, 3682
Bse1I ACTGG 8 cut(s) 814, 955, 2529, 2557, 2629, 2704, 3080, 3712
Bse3DI GCAATG 1 cut(s) 810
BseAI TCCGGA 1 cut(s) 3409
BseBI CCWGG 2 cut(s) 478, 3159
BseDI CCNNGG 6 cut(s) 477, 560, 1119, 1165, 2983, 3158
BseLI CCNNNNNNNGG 7 cut(s) 284, 386, 1489, 2049, 2114, 2535, 3682
BseMI GCAATG 1 cut(s) 810
BseMII CTCAG 5 cut(s) 540, 1764, 1891, 3518, 3660
BseNI ACTGG 8 cut(s) 814, 955, 2529, 2557, 2629, 2704, 3080, 3712
BseRI GAGGAG 2 cut(s) 1346, 2114
BseSI GKGCMC 2 cut(s) 3027, 3677
BseX3I CGGCCG 1 cut(s) 563
BseXI GCAGC 7 cut(s) 77, 514, 952, 1642, 1664, 1820, 2731
BseYI CCCAGC 3 cut(s) 177, 187, 1321
BsgI GTGCAG 2 cut(s) 2600, 3628
Bsh1236I CGCG 1 cut(s) 52
Bsh1285I CGRYCG 1 cut(s) 566
BshFI GGCC 4 cut(s) 565, 2036, 2107, 2164
BshNI GGYRCC 1 cut(s) 3024
BsiEI CGRYCG 1 cut(s) 566
BsiHKAI GWGCWC 3 cut(s) 1942, 2253, 3633
BsiHKCI CYCGRG 1 cut(s) 1399
BsiSI CCGG 6 cut(s) 1083, 2140, 2891, 3410, 3593, 3626
BslFI GGGAC 1 cut(s) 709
BslI CCNNNNNNNGG 7 cut(s) 284, 386, 1489, 2049, 2114, 2535, 3682
BsmAI GTCTC 5 cut(s) 537, 887, 1637, 3168, 3238
BsmBI CGTCTC 1 cut(s) 537
BsmFI GGGAC 1 cut(s) 709
BsmI GAATGC 4 cut(s) 1000, 1628, 2022, 3220
BsnI GGCC 4 cut(s) 565, 2036, 2107, 2164
Bso31I GGTCTC 2 cut(s) 1637, 3168
BsoBI CYCGRG 1 cut(s) 1399
Bsp119I TTCGAA 1 cut(s) 2026
Bsp1286I GDGCHC 5 cut(s) 1942, 2253, 3027, 3633, 3677
Bsp13I TCCGGA 1 cut(s) 3409
Bsp1720I GCTNAGC 1 cut(s) 549
Bsp19I CCATGG 2 cut(s) 1119, 2983
BspACI CCGC 7 cut(s) 14, 52, 536, 1626, 2050, 2146, 3115
BspANI GGCC 4 cut(s) 565, 2036, 2107, 2164
BspCNI CTCAG 5 cut(s) 541, 1765, 1890, 3517, 3661
BspEI TCCGGA 1 cut(s) 3409
BspFNI CGCG 1 cut(s) 52
BspHI TCATGA 5 cut(s) 1341, 1551, 1576, 2176, 2202
BspLI GGNNCC 3 cut(s) 864, 2144, 3026
BspMAI CTGCAG 1 cut(s) 67
BspPI GGATC 4 cut(s) 551, 1346, 1501, 3317
BspT104I TTCGAA 1 cut(s) 2026
BspT107I GGYRCC 1 cut(s) 3024
BspTI CTTAAG 2 cut(s) 2456, 2495
BspTNI GGTCTC 2 cut(s) 1637, 3168
BsrDI GCAATG 1 cut(s) 810
BsrI ACTGG 8 cut(s) 814, 955, 2529, 2557, 2629, 2704, 3080, 3712
BssECI CCNNGG 6 cut(s) 477, 560, 1119, 1165, 2983, 3158
BssNAI GTATAC 1 cut(s) 2285
BssNI GRCGYC 3 cut(s) 1559, 1809, 2547
BssT1I CCWWGG 3 cut(s) 1119, 1165, 2983
Bst1107I GTATAC 1 cut(s) 2285
Bst2UI CCWGG 2 cut(s) 478, 3159
Bst4CI ACNGT 6 cut(s) 635, 700, 1162, 1588, 1988, 3102
Bst6I CTCTTC 2 cut(s) 885, 3479
BstACI GRCGYC 3 cut(s) 1559, 1809, 2547
BstAFI CTTAAG 2 cut(s) 2456, 2495
BstAPI GCANNNNNTGC 1 cut(s) 1479
BstBAI YACGTR 2 cut(s) 2288, 3697
BstBI TTCGAA 1 cut(s) 2026
BstC8I GCNNGC 5 cut(s) 1116, 1186, 1358, 2749, 2753
BstDSI CCRYGG 2 cut(s) 1119, 2983
BstFNI CGCG 1 cut(s) 52
BstHHI GCGC 1 cut(s) 2326
BstMAI GTCTC 5 cut(s) 537, 887, 1637, 3168, 3238
BstMCI CGRYCG 1 cut(s) 566
BstMWI GCNNNNNNNGC 7 cut(s) 1479, 1649, 2221, 2352, 3413, 3576, 3817
BstNI CCWGG 2 cut(s) 478, 3159
BstNSI RCATGY 3 cut(s) 1217, 1360, 2751
BstSCI CCNGG 2 cut(s) 476, 3157
BstSFI CTRYAG 2 cut(s) 63, 120
BstSLI GKGCMC 2 cut(s) 3027, 3677
BstUI CGCG 1 cut(s) 52
BstV1I GCAGC 7 cut(s) 77, 514, 952, 1642, 1664, 1820, 2731
BstV2I GAAGAC 8 cut(s) 240, 318, 606, 1044, 1562, 1785, 2958, 3519
BstX2I RGATCY 5 cut(s) 556, 1506, 2560, 3305, 3619
BstXI CCANNNNNNTGG 1 cut(s) 1238
BstYI RGATCY 5 cut(s) 556, 1506, 2560, 3305, 3619
BstZ17I GTATAC 1 cut(s) 2285
BstZI CGGCCG 1 cut(s) 563
BsuI GTATCC 1 cut(s) 1282
BsuRI GGCC 4 cut(s) 565, 2036, 2107, 2164
BtgI CCRYGG 2 cut(s) 1119, 2983
BtrI CACGTC 1 cut(s) 679
BtsI GCAGTG 1 cut(s) 3536
BtsIMutI CAGTG 8 cut(s) 821, 867, 962, 1158, 2550, 2999, 3536, 3645
Cac8I GCNNGC 5 cut(s) 1116, 1186, 1358, 2749, 2753
CaiI CAGNNNCTG 1 cut(s) 194
CciI TCATGA 5 cut(s) 1341, 1551, 1576, 2176, 2202
CfoI GCGC 1 cut(s) 2326
Cfr13I GGNCC 3 cut(s) 163, 2052, 3401
CpoI CGGWCCG 1 cut(s) 2052
CseI GACGC 2 cut(s) 519, 1817
CspI CGGWCCG 1 cut(s) 2052
DraI TTTAAA 2 cut(s) 972, 2715
DriI GACNNNNNGTC 2 cut(s) 37, 1813
EaeI YGGCCR 4 cut(s) 563, 2034, 2105, 2162
EagI CGGCCG 1 cut(s) 563
Eam1104I CTCTTC 2 cut(s) 885, 3479
Eam1105I GACNNNNNGTC 2 cut(s) 37, 1813
EarI CTCTTC 2 cut(s) 885, 3479
EciI GGCGGA 1 cut(s) 525
Ecl136II GAGCTC 1 cut(s) 2251
EclXI CGGCCG 1 cut(s) 563
Eco130I CCWWGG 3 cut(s) 1119, 1165, 2983
Eco24I GRGCYC 1 cut(s) 2253
Eco31I GGTCTC 2 cut(s) 1637, 3168
Eco32I GATATC 2 cut(s) 1858, 2589
Eco47I GGWCC 3 cut(s) 163, 2052, 3401
Eco52I CGGCCG 1 cut(s) 563
Eco53kI GAGCTC 1 cut(s) 2251
Eco88I CYCGRG 1 cut(s) 1399
EcoICRI GAGCTC 1 cut(s) 2251
EcoRI GAATTC 1 cut(s) 1410
EcoRII CCWGG 2 cut(s) 476, 3157
EcoRV GATATC 2 cut(s) 1858, 2589
EcoT14I CCWWGG 3 cut(s) 1119, 1165, 2983
EcoT22I ATGCAT 1 cut(s) 2804
EcoT38I GRGCYC 1 cut(s) 2253
ErhI CCWWGG 3 cut(s) 1119, 1165, 2983
Esp3I CGTCTC 1 cut(s) 537
FalI AAGNNNNNCTT 8 cut(s) 303, 335, 495, 527, 1227, 1259, 1805, 1837
FaqI GGGAC 1 cut(s) 709
FauNDI CATATG 1 cut(s) 3392
FbaI TGATCA 3 cut(s) 657, 1150, 1249
FblI GTMKAC 4 cut(s) 88, 128, 2284, 3368
Fnu4HI GCNGC 7 cut(s) 66, 528, 966, 1653, 1656, 1834, 2745
FriOI GRGCYC 1 cut(s) 2253
Fsp4HI GCNGC 7 cut(s) 66, 528, 966, 1653, 1656, 1834, 2745
FspBI CTAG 9 cut(s) 686, 1971, 2096, 2352, 2933, 3281, 3374, 3383, 3788
GlaI GCGC 1 cut(s) 2325
GluI GCNGC 7 cut(s) 66, 528, 966, 1653, 1656, 1834, 2745
GsaI CCCAGC 3 cut(s) 181, 191, 1325
GsuI CTGGAG 2 cut(s) 1638, 3695
HaeIII GGCC 4 cut(s) 565, 2036, 2107, 2164
HapII CCGG 6 cut(s) 1083, 2140, 2891, 3410, 3593, 3626
HgaI GACGC 2 cut(s) 519, 1817
HhaI GCGC 1 cut(s) 2326
Hin1I GRCGYC 3 cut(s) 1559, 1809, 2547
Hin6I GCGC 1 cut(s) 2324
HinP1I GCGC 1 cut(s) 2324
HincII GTYRAC 4 cut(s) 89, 129, 1762, 2697
HindII GTYRAC 4 cut(s) 89, 129, 1762, 2697
HindIII AAGCTT 4 cut(s) 909, 1965, 2213, 2222
HinfI GANTC 9 cut(s) 125, 734, 987, 1316, 1535, 1580, 2470, 2863, 3549
HpaII CCGG 6 cut(s) 1083, 2140, 2891, 3410, 3593, 3626
HphI GGTGA 6 cut(s) 439, 487, 1450, 2075, 3158, 3223
Hpy188I TCNGA 7 cut(s) 265, 1017, 1528, 2056, 2248, 2740, 3310
Hpy99I CGWCG 3 cut(s) 93, 1021, 1564
HpyCH4III ACNGT 6 cut(s) 635, 700, 1162, 1588, 1988, 3102
HpyCH4IV ACGT 6 cut(s) 678, 1559, 1782, 2287, 2547, 3696
HpyF10VI GCNNNNNNNGC 7 cut(s) 1479, 1649, 2221, 2352, 3413, 3576, 3817
HpySE526I ACGT 6 cut(s) 678, 1559, 1782, 2287, 2547, 3696
Hsp92I GRCGYC 3 cut(s) 1559, 1809, 2547
HspAI GCGC 1 cut(s) 2324
Kpn2I TCCGGA 1 cut(s) 3409
Ksp22I TGATCA 3 cut(s) 657, 1150, 1249
LmnI GCTCC 5 cut(s) 196, 2148, 2256, 2835, 3412
Lsp1109I GCAGC 7 cut(s) 77, 514, 952, 1642, 1664, 1820, 2731
LweI GCATC 9 cut(s) 356, 1991, 2161, 2650, 2731, 2803, 2811, 3115, 3403
MaeI CTAG 9 cut(s) 686, 1971, 2096, 2352, 2933, 3281, 3374, 3383, 3788
MaeII ACGT 6 cut(s) 678, 1559, 1782, 2287, 2547, 3696
MfeI CAATTG 2 cut(s) 1863, 2881
MflI RGATCY 5 cut(s) 556, 1506, 2560, 3305, 3619
MhlI GDGCHC 5 cut(s) 1942, 2253, 3027, 3633, 3677
MlsI TGGCCA 3 cut(s) 2036, 2107, 2164
MluNI TGGCCA 3 cut(s) 2036, 2107, 2164
MlyI GAGTC 3 cut(s) 134, 2857, 3558
MmeI TCCRAC 6 cut(s) 66, 1040, 1171, 2523, 2688, 3489
Mox20I TGGCCA 3 cut(s) 2036, 2107, 2164
Mph1103I ATGCAT 1 cut(s) 2804
MroI TCCGGA 1 cut(s) 3409
MroXI GAANNNNTTC 2 cut(s) 1547, 2304
MscI TGGCCA 3 cut(s) 2036, 2107, 2164
MseI TTAA 8 cut(s) 83, 918, 971, 2429, 2457, 2496, 2714, 3585
MslI CAYNNNNRTG 3 cut(s) 653, 2009, 3762
Msp20I TGGCCA 3 cut(s) 2036, 2107, 2164
MspA1I CMGCKG 2 cut(s) 548, 1655
MspCI CTTAAG 2 cut(s) 2456, 2495
MspI CCGG 6 cut(s) 1083, 2140, 2891, 3410, 3593, 3626
MspR9I CCNGG 2 cut(s) 478, 3159
MunI CAATTG 2 cut(s) 1863, 2881
Mva1269I GAATGC 4 cut(s) 1000, 1628, 2022, 3220
MvaI CCWGG 2 cut(s) 478, 3159
MvnI CGCG 1 cut(s) 52
MwoI GCNNNNNNNGC 7 cut(s) 1479, 1649, 2221, 2352, 3413, 3576, 3817
NcoI CCATGG 2 cut(s) 1119, 2983
NdeI CATATG 1 cut(s) 3392
NlaIV GGNNCC 3 cut(s) 864, 2144, 3026
NmeAIII GCCGAG 2 cut(s) 541, 3796
NmuCI GTSAC 6 cut(s) 445, 1156, 1662, 2288, 2548, 2911
NsiI ATGCAT 1 cut(s) 2804
NspI RCATGY 3 cut(s) 1217, 1360, 2751
NspV TTCGAA 1 cut(s) 2026
OliI CACNNNNGTG 1 cut(s) 653
PaeI GCATGC 2 cut(s) 1360, 2751
PaeR7I CTCGAG 1 cut(s) 1399
PagI TCATGA 5 cut(s) 1341, 1551, 1576, 2176, 2202
PciI ACATGT 1 cut(s) 1213
PctI GAATGC 4 cut(s) 1000, 1628, 2022, 3220
PdmI GAANNNNTTC 2 cut(s) 1547, 2304
PfeI GAWTC 6 cut(s) 734, 987, 1316, 1535, 1580, 2470
PflFI GACNNNGTC 1 cut(s) 3548
PflMI CCANNNNNTGG 2 cut(s) 386, 1489
PkrI GCNGC 7 cut(s) 67, 529, 967, 1654, 1657, 1835, 2746
PleI GAGTC 3 cut(s) 133, 2857, 3557
PpsI GAGTC 3 cut(s) 133, 2857, 3557
Ppu21I YACGTR 2 cut(s) 2288, 3697
PscI ACATGT 1 cut(s) 1213
PsiI TTATAA 1 cut(s) 1364
Psp124BI GAGCTC 1 cut(s) 2253
Psp6I CCWGG 2 cut(s) 476, 3157
PspFI CCCAGC 3 cut(s) 177, 187, 1321
PspGI CCWGG 2 cut(s) 476, 3157
PspN4I GGNNCC 3 cut(s) 864, 2144, 3026
PspPI GGNCC 3 cut(s) 163, 2052, 3401
PstI CTGCAG 1 cut(s) 67
PstNI CAGNNNCTG 1 cut(s) 194
PsuI RGATCY 5 cut(s) 556, 1506, 2560, 3305, 3619
PsyI GACNNNGTC 1 cut(s) 3548
PvuII CAGCTG 2 cut(s) 548, 1655
RseI CAYNNNNRTG 3 cut(s) 653, 2009, 3762
Rsr2I CGGWCCG 1 cut(s) 2052
RsrII CGGWCCG 1 cut(s) 2052
SacI GAGCTC 1 cut(s) 2253
SalI GTCGAC 2 cut(s) 87, 127
SaqAI TTAA 8 cut(s) 83, 918, 971, 2429, 2457, 2496, 2714, 3585
SatI GCNGC 7 cut(s) 66, 528, 966, 1653, 1656, 1834, 2745
Sau96I GGNCC 3 cut(s) 163, 2052, 3401
ScaI AGTACT 1 cut(s) 1068
SchI GAGTC 3 cut(s) 134, 2857, 3558
ScrFI CCNGG 2 cut(s) 478, 3159
SduI GDGCHC 5 cut(s) 1942, 2253, 3027, 3633, 3677
SfaNI GCATC 9 cut(s) 356, 1991, 2161, 2650, 2731, 2803, 2811, 3115, 3403
SfcI CTRYAG 2 cut(s) 63, 120
Sfr274I CTCGAG 1 cut(s) 1399
SfuI TTCGAA 1 cut(s) 2026
SinI GGWCC 3 cut(s) 163, 2052, 3401
SlaI CTCGAG 1 cut(s) 1399
SmiMI CAYNNNNRTG 3 cut(s) 653, 2009, 3762
SmlI CTYRAG 6 cut(s) 1399, 2456, 2465, 2495, 3128, 3340
SmoI CTYRAG 6 cut(s) 1399, 2456, 2465, 2495, 3128, 3340
SpeI ACTAGT 1 cut(s) 3382
SphI GCATGC 2 cut(s) 1360, 2751
SsiI CCGC 7 cut(s) 14, 52, 536, 1626, 2050, 2146, 3115
SspI AATATT 1 cut(s) 2234
SspMI CTAG 9 cut(s) 686, 1971, 2096, 2352, 2933, 3281, 3374, 3383, 3788
SstI GAGCTC 1 cut(s) 2253
StyD4I CCNGG 2 cut(s) 476, 3157
StyI CCWWGG 3 cut(s) 1119, 1165, 2983
TaaI ACNGT 6 cut(s) 635, 700, 1162, 1588, 1988, 3102
TaiI ACGT 6 cut(s) 681, 1562, 1785, 2290, 2550, 3699
TaqI TCGA 7 cut(s) 88, 128, 1400, 2026, 2591, 3230, 3663
TaqII GACCGA 1 cut(s) 180
TatI WGTACW 4 cut(s) 1066, 1220, 2769, 2937
TfiI GAWTC 6 cut(s) 734, 987, 1316, 1535, 1580, 2470
Tru1I TTAA 8 cut(s) 83, 918, 971, 2429, 2457, 2496, 2714, 3585
Tru9I TTAA 8 cut(s) 83, 918, 971, 2429, 2457, 2496, 2714, 3585
TscAI CASTG 8 cut(s) 821, 874, 962, 1165, 2557, 3006, 3536, 3652
TseFI GTSAC 6 cut(s) 445, 1156, 1662, 2288, 2548, 2911
TseI GCWGC 7 cut(s) 65, 527, 965, 1652, 1655, 1833, 2744
Tsp45I GTSAC 6 cut(s) 445, 1156, 1662, 2288, 2548, 2911
TspGWI ACGGA 4 cut(s) 106, 1034, 2431, 3511
TspRI CASTG 8 cut(s) 821, 874, 962, 1165, 2557, 3006, 3536, 3652
Tth111I GACNNNGTC 1 cut(s) 3548
Van91I CCANNNNNTGG 2 cut(s) 386, 1489
Vha464I CTTAAG 2 cut(s) 2456, 2495
VpaK11BI GGWCC 3 cut(s) 163, 2052, 3401
XbaI TCTAGA 3 cut(s) 1970, 3280, 3373
XceI RCATGY 3 cut(s) 1217, 1360, 2751
XcmI CCANNNNNNNNNTGG 1 cut(s) 195
XhoI CTCGAG 1 cut(s) 1399
XmiI GTMKAC 4 cut(s) 88, 128, 2284, 3368
XmnI GAANNNNTTC 2 cut(s) 1547, 2304
XspI CTAG 9 cut(s) 686, 1971, 2096, 2352, 2933, 3281, 3374, 3383, 3788
ZraI GACGTC 2 cut(s) 1560, 2548
ZrmI AGTACT 1 cut(s) 1068
Zsp2I ATGCAT 1 cut(s) 2804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.