Rw5G039580

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000085
Physical Location & Seq
Forward (+)
69827785 .. 69831801
4017 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G039580.1

Sequence Viewer

Length: 3012 bp
ATGTATCGTTCTGATGATTATTTGACACTTCTTCCCTGTAGGTCTGATCAAGAACTTGTTGACAACATTGTGGAAACTGTGTGCAGTAAAGTGCGACCTATTGATATAGAGTGGGAAATGTGCACCGGAGATTTTGTAGAATTTGAAGCAACAAGACAAGCCATGGACAACATAATGAGGGCTCTTCAAGATTATGAGGTCACGACCATTGGAGTCTTTGGAATTGGAGGCGTTGGCAAGACAAGCATGGTGAAACATGTCGCTGCACAAGCCCGGAAAATTGGGCTTTTTCATCACGTGATTATGGCCGTTGTATCCCAGACACCCAACTTGAGAAATATTCAAGGCACACTGGCCGATCTGCTGGGCTTAAAATTGCAGGAGGAGACGGATAGTGGAAGAGCAGCTAGAATAGCGGATAAGATATTGAGAGGATATAAGATCCTCATAATCTTGGACGACATTTGGAAGAGAGAAGATTTGTCTAGAATCGGAATACCGAACCATACAGGACTTCAAAGATGCAATTCAAAAATCATACTCACCACAAGGAGAAAGAATGTCTGTCATTCCATGGGGAGCCAAGCAAGCATTCCTCTCCAAGTTCTGTCAGAAGATGATGCTTGGGACCTGTTTGTGAAGAAAGCAAGAAAGTCTTTTCAAGAATCACCCAACTTTTATGATGTAGCGAGGACGGTAGCTAAAGAATGTGCTGGTCTTCCGGTTGCATTGGTAGCAGTTGCAAGTGCACTTGGAGATAAAGACTTGGATGATTGGACAGAAGCAGCTCGACGACTAAAGACTGCTCAACCTGCCAACTATGAAGATGATGGAGATGTGTTCGGATGTATCAAGTTAAGCTATGATTACTTGAGACCTGATGATGACAACATTGCCAAGTCATTCTTCTTGCTTTGCTGTCTATTCCCTGAAGATTTTGATATCGAGATCGAAGACTTGATGGTGTATGCAATTGGGAAAGGAATACTTCAAGATGCCCACACAATAGAAGAAGCCAGAGCCAAAGCCCATTCGGTGGTTAAGTACCTGAAAGCTTCTAGCTTGCTTTTGGACAGTGCATACCCTGGATTCGTGAAGATGCATGATGTCATTCGAGATGTGGCCATATCAATTTCATCATCGGAAGATGGCCCACGGTTTTTGGTTAAAGCTGGTTGTGAATTAAGGGATTGGCCATCGATTAATATTGCACATGAAGGCTACTCTGCAATCTCGCTAATGAGGAATGAAATTCACGAGCTTCCCAAAACGTTGGTATGTCCAAAACTCCAGATTTTATTACTACAGAGCAATAGTATAAATGAGATTCCAAACCCTTTCTTTCAAAGTCCCAATGAATTGAGGGTCTTAGATCTCAGCCGGACTGGTATTTCGTTTCTACCCTCATCATCAAGTCTTCTAACCAACCTCCAAGCTTTGTATTTAGATGAATGCAAGAGAATAGTTGATATATCCATGCTCCGAAAACTGAAGAACCTTCAGATTCTTAGTATGAAGAACCTTGACATTATTAATACGGGAGGAACTCGTCAAAATGCATTGCCGAAAGAAATAGGACGCTTAACCAAGCTTAAGATGTTGGATGTTACGGATGCATGTTTTGAAACAGTTCCATCTAAAGTGATATCAAACTTGAATGGATTAGAAGAATTGTACATGAAATGTGACTTTTGTGACTGGGGCAGTAGAATAGTTGAGGGTTTATTTTCTTTTGTTTATGGCAATAATGCTAGCTTTGATGAGTTAACAGGCTTATCACGCTTAAACAGTGTGAAAGTTCACATATCTGATGTAGAACGCTTGCCCCGAAAAGTTGGATTCAATCCCAATTGGGTCAACTTTGATATATGTATCAGCAGAAAAGAATTTATGAGACAGTCCTATTACGGACGCTTTTTCGAGAGGAAACTAACAGTCAAGTCGGATCCACGCCAACATCTCTTTCGGTATGTGACTCTGAACACAAGCATGAAGAGTTTACCAGATTGGTTTATCAATGCCGTGACAGAGAAAGCAGAGATACTGGAGTACATAGGGGACGGTGGCTTAGAGAACCCATTTGGACATTTGGACATTCTAGAGGAATACGACCTTGGGAGATTACATGTACTGAAGTGTCTCGCTGTAATTGAGTGCCGTCTGTATGGAGCGTTGACGAACCCATTAACAAGGGTTCTAAATACACCTGTGTTTGAGAGCTTGATAGATTTGTATCTGGAAGGGGTCCATGGCCTGCGGGGATTATTTTTCGATGAATTACCAGCTGGGTCTCTGTGCAATCTTAAGTTGTTAAATGTGAGAAATTGCCTGGAATTGTTGGGGATACTTTTAGAATCACATATGTTGCAGATGCTACCAAAACTGGAAATACTACATTGCGAGAACACGGAGGATGCGAGAGAAACCGCAATGCTTTATGTGTTTGAATTTGGATGGCTGGAGCCAGAACAAATTGTCTTACCAAAACTGAGAGAGATAACATTATGTCATCTTGTGAACCTCAAAAGCATATGCATTGGTCATCCTCCTAGTGGAGCCTTCCGTAATCTTAACAGTCTGGCAATTCGGGGCTGCCGCAAACTGAAATATCTGTTCGAGGACAATGCAGCTCAATCTCTTTTCCAATTGGAACTCCTTTGGGTGGAGTGCTCTGACCTGGAAACGGTTATTGAATCAAGACAGGAAACAGAGATCAACAACCTTGTTTTTCCAAAATTGAAGGAATTATATTTAAATAGTCTTCCACATCTCACAGGGATCTACAGACAAAAGACGGTTGTGTGCCCTTCATTGGAACACTTGTACGTGTACAGTTGTCCCGGACTTGCAAACCTTGCTGCTTCTGACTTCCAAAGCAAGAACCACGTCCAAGCCAATAATGAAGGACATTTTGCAATCATATTTGAACGATTGTTCCACCGTCAATTCAATCCAGAAGAGTTTCCTCAGTTCTTCTCCAAAAAAGAAAGAGATTTCTCAATTGGCTCTTAA

Protein Analysis

1003

Amino Acids

113.72

Weight (kDa)

5.69

Isoelectric Point (pI)

46.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 53 - 217 7.2e-29 NB-ARC domain
LRR_8 PF13855 428 - 485 3.8e-08 Leucine rich repeat
LRR_14 PF23598 429 - 507 1e-05 Leucine-rich repeat region
LRR_RPS2 PF23247 753 - 889 5.6e-18 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 820
AccB7I CCANNNNNTGG 1 cut(s) 1036
AciI CCGC 4 cut(s) 416, 2257, 2427, 2596
AclI AACGTT 1 cut(s) 1271
AclWI GGATC 4 cut(s) 436, 1940, 1953, 2786
AcoI YGGCCR 4 cut(s) 306, 354, 1122, 1193
AcsI RAATTY 4 cut(s) 140, 1251, 1886, 2447
AcuI CTGAAG 4 cut(s) 951, 1484, 1511, 2153
AcvI CACGTG 1 cut(s) 298
AfaI GTAC 6 cut(s) 1046, 1676, 2051, 2130, 2825, 2831
AfiI CCNNNNNNNGG 5 cut(s) 1036, 2552, 2662, 2683, 2812
AflII CTTAAG 2 cut(s) 1592, 2303
AflIII ACRYGT 3 cut(s) 256, 2125, 2826
AjiI CACGTC 1 cut(s) 2887
AjnI CCWGG 3 cut(s) 1084, 2328, 2676
AjuI GAANNNNNNNTTGG 8 cut(s) 576, 608, 890, 922, 1947, 1979, 2097, 2129
AloI GAACNNNNNNTCC 2 cut(s) 2919, 2951
Alw21I GWGCWC 3 cut(s) 125, 751, 2672
Alw26I GTCTC 5 cut(s) 380, 868, 1888, 2144, 2295
Alw44I GTGCAC 2 cut(s) 121, 747
AlwI GGATC 4 cut(s) 436, 1940, 1953, 2786
AoxI GGCC 6 cut(s) 306, 354, 1122, 1150, 1193, 2251
ApaLI GTGCAC 2 cut(s) 121, 747
ApeKI GCWGC 6 cut(s) 263, 404, 785, 2592, 2627, 2858
ApoI RAATTY 4 cut(s) 140, 1251, 1886, 2447
ArsI GACNNNNNNTTYG 2 cut(s) 1375, 1407
AseI ATTAAT 2 cut(s) 1203, 1533
Asp700I GAANNNNTTC 2 cut(s) 1629, 2961
AspS9I GGNCC 3 cut(s) 628, 1151, 2245
AsuC2I CCSGG 2 cut(s) 274, 2841
AsuHPI GGTGA 3 cut(s) 262, 535, 660
AsuNHI GCTAGC 1 cut(s) 1751
AvaII GGWCC 2 cut(s) 628, 2245
BaeGI GKGCMC 3 cut(s) 125, 751, 2807
BaeI ACNNNNGTAYC 2 cut(s) 2033, 2066
BalI TGGCCA 2 cut(s) 1124, 1195
BamHI GGATCC 1 cut(s) 1945
BanII GRGCYC 1 cut(s) 184
BauI CACGAG 1 cut(s) 1256
BbrPI CACGTG 1 cut(s) 298
BbsI GAAGAC 4 cut(s) 710, 960, 1409, 2753
Bbv12I GWGCWC 3 cut(s) 125, 751, 2672
BbvI GCAGC 6 cut(s) 250, 416, 797, 2579, 2639, 2845
BccI CCATC 6 cut(s) 824, 955, 1142, 1204, 1642, 2448
BceAI ACGGC 3 cut(s) 293, 2006, 2142
BcgI CGANNNNNNTGC 2 cut(s) 2606, 2640
BciT130I CCWGG 3 cut(s) 1086, 2330, 2678
BciVI GTATCC 2 cut(s) 325, 2337
BclI TGATCA 1 cut(s) 46
BcnI CCSGG 2 cut(s) 274, 2841
BcoDI GTCTC 5 cut(s) 380, 868, 1888, 2144, 2295
BfaI CTAG 6 cut(s) 408, 486, 1059, 1752, 2099, 2550
BfmI CTRYAG 3 cut(s) 37, 1304, 2782
BfrI CTTAAG 2 cut(s) 1592, 2303
BfuAI ACCTGC 1 cut(s) 820
BfuI GTATCC 2 cut(s) 325, 2337
BglII AGATCT 1 cut(s) 1372
BisI GCNGC 7 cut(s) 264, 405, 786, 2593, 2596, 2628, 2859
BlsI GCNGC 7 cut(s) 265, 406, 787, 2594, 2597, 2629, 2860
Bme1390I CCNGG 5 cut(s) 274, 1086, 2330, 2678, 2841
Bme18I GGWCC 2 cut(s) 628, 2245
BmgBI CACGTC 1 cut(s) 2887
BmgT120I GGNCC 3 cut(s) 628, 1151, 2245
BmiI GGNNCC 6 cut(s) 581, 629, 1947, 2246, 2463, 2557
BmrFI CCNGG 5 cut(s) 274, 1086, 2330, 2678, 2841
BmrI ACTGGG 1 cut(s) 1708
BmsI GCATC 7 cut(s) 512, 610, 985, 1089, 1603, 2361, 2404
BmtI GCTAGC 1 cut(s) 1755
BmuI ACTGGG 1 cut(s) 1708
BpiI GAAGAC 4 cut(s) 710, 960, 1409, 2753
BpmI CTGGAG 3 cut(s) 1274, 2066, 2480
BpuEI CTTGAG 2 cut(s) 352, 892
BpuMI CCSGG 2 cut(s) 274, 2841
Bsa29I ATCGAT 1 cut(s) 1199
BsaAI YACGTR 2 cut(s) 298, 2827
BsaBI GATNNNNATC 1 cut(s) 2232
BsaI GGTCTC 2 cut(s) 868, 2295
BsaJI CCNNGG 6 cut(s) 162, 573, 1084, 1154, 2113, 2248
BsaWI WCCGGW 2 cut(s) 125, 721
BsaXI ACNNNNNCTCC 2 cut(s) 120, 150
Bsc4I CCNNNNNNNGG 5 cut(s) 1036, 2552, 2662, 2683, 2812
Bse1I ACTGG 5 cut(s) 357, 1390, 1703, 2049, 2388
Bse3DI GCAATG 4 cut(s) 891, 1559, 2395, 2436
Bse8I GATNNNNATC 1 cut(s) 2232
BseBI CCWGG 3 cut(s) 1086, 2330, 2678
BseCI ATCGAT 1 cut(s) 1199
BseDI CCNNGG 6 cut(s) 162, 573, 1084, 1154, 2113, 2248
BseGI GGATG 7 cut(s) 775, 851, 1609, 1618, 2419, 2459, 2542
BseJI GATNNNNATC 1 cut(s) 2232
BseLI CCNNNNNNNGG 5 cut(s) 1036, 2552, 2662, 2683, 2812
BseMI GCAATG 4 cut(s) 891, 1559, 2395, 2436
BseMII CTCAG 3 cut(s) 1390, 2480, 2981
BseNI ACTGG 5 cut(s) 357, 1390, 1703, 2049, 2388
BseRI GAGGAG 1 cut(s) 398
BseSI GKGCMC 3 cut(s) 125, 751, 2807
BseXI GCAGC 6 cut(s) 250, 416, 797, 2579, 2639, 2845
BseYI CCCAGC 2 cut(s) 364, 2285
BsgI GTGCAG 2 cut(s) 103, 249
BshFI GGCC 6 cut(s) 308, 356, 1124, 1152, 1195, 2253
BshVI ATCGAT 1 cut(s) 1199
BsiHKAI GWGCWC 3 cut(s) 125, 751, 2672
BsiSI CCGG 5 cut(s) 126, 274, 722, 1381, 2841
BslFI GGGAC 4 cut(s) 641, 1335, 2072, 2823
BslI CCNNNNNNNGG 5 cut(s) 1036, 2552, 2662, 2683, 2812
BsmAI GTCTC 5 cut(s) 380, 868, 1888, 2144, 2295
BsmBI CGTCTC 1 cut(s) 380
BsmFI GGGAC 4 cut(s) 641, 1335, 2072, 2823
BsmI GAATGC 2 cut(s) 591, 1457
BsnI GGCC 6 cut(s) 308, 356, 1124, 1152, 1195, 2253
Bso31I GGTCTC 2 cut(s) 868, 2295
Bsp1286I GDGCHC 5 cut(s) 125, 184, 751, 2672, 2807
Bsp1407I TGTACA 2 cut(s) 1674, 2829
Bsp143I GATC 8 cut(s) 46, 358, 441, 948, 1372, 1945, 2712, 2778
Bsp19I CCATGG 3 cut(s) 162, 573, 2248
BspACI CCGC 4 cut(s) 416, 2257, 2427, 2596
BspANI GGCC 6 cut(s) 308, 356, 1124, 1152, 1195, 2253
BspCNI CTCAG 3 cut(s) 1389, 2481, 2980
BspDI ATCGAT 1 cut(s) 1199
BspLI GGNNCC 6 cut(s) 581, 629, 1947, 2246, 2463, 2557
BspMI ACCTGC 1 cut(s) 820
BspOI GCTAGC 1 cut(s) 1755
BspPI GGATC 4 cut(s) 436, 1940, 1953, 2786
BspQI GCTCTTC 2 cut(s) 189, 394
BspTI CTTAAG 2 cut(s) 1592, 2303
BspTNI GGTCTC 2 cut(s) 868, 2295
BsrDI GCAATG 4 cut(s) 891, 1559, 2395, 2436
BsrGI TGTACA 2 cut(s) 1674, 2829
BsrI ACTGG 5 cut(s) 357, 1390, 1703, 2049, 2388
BssECI CCNNGG 6 cut(s) 162, 573, 1084, 1154, 2113, 2248
BssMI GATC 8 cut(s) 46, 358, 441, 948, 1372, 1945, 2712, 2778
BssSI CACGAG 1 cut(s) 1256
BssT1I CCWWGG 4 cut(s) 162, 573, 2113, 2248
Bst2BI CACGAG 1 cut(s) 1256
Bst2UI CCWGG 3 cut(s) 1086, 2330, 2678
Bst6I CTCTTC 5 cut(s) 189, 394, 464, 1988, 2952
BstAFI CTTAAG 2 cut(s) 1592, 2303
BstAPI GCANNNNNTGC 1 cut(s) 2855
BstAUI TGTACA 2 cut(s) 1674, 2829
BstBAI YACGTR 2 cut(s) 298, 2827
BstC8I GCNNGC 5 cut(s) 589, 1064, 1753, 1823, 2255
BstDEI CTNAG 6 cut(s) 1369, 1376, 1508, 2068, 2489, 2967
BstDSI CCRYGG 4 cut(s) 162, 573, 1154, 2248
BstF5I GGATG 7 cut(s) 775, 851, 1609, 1618, 2419, 2459, 2542
BstKTI GATC 8 cut(s) 49, 361, 444, 951, 1375, 1948, 2715, 2781
BstMAI GTCTC 5 cut(s) 380, 868, 1888, 2144, 2295
BstMBI GATC 8 cut(s) 46, 358, 441, 948, 1372, 1945, 2712, 2778
BstMWI GCNNNNNNNGC 8 cut(s) 243, 269, 413, 588, 734, 812, 1779, 2855
BstNI CCWGG 3 cut(s) 1086, 2330, 2678
BstNSI RCATGY 3 cut(s) 260, 1620, 2129
BstSCI CCNGG 5 cut(s) 272, 1084, 2328, 2676, 2839
BstSFI CTRYAG 3 cut(s) 37, 1304, 2782
BstSLI GKGCMC 3 cut(s) 125, 751, 2807
BstV1I GCAGC 6 cut(s) 250, 416, 797, 2579, 2639, 2845
BstV2I GAAGAC 4 cut(s) 710, 960, 1409, 2753
BstX2I RGATCY 4 cut(s) 441, 1372, 1945, 2778
BstXI CCANNNNNNTGG 1 cut(s) 1273
BstYI RGATCY 4 cut(s) 441, 1372, 1945, 2778
Bsu15I ATCGAT 1 cut(s) 1199
BsuI GTATCC 2 cut(s) 325, 2337
BsuRI GGCC 6 cut(s) 308, 356, 1124, 1152, 1195, 2253
BsuTUI ATCGAT 1 cut(s) 1199
BtgI CCRYGG 4 cut(s) 162, 573, 1154, 2248
BtrI CACGTC 1 cut(s) 2887
BtsCI GGATG 7 cut(s) 775, 851, 1609, 1618, 2419, 2459, 2542
BtsIMutI CAGTG 3 cut(s) 350, 1081, 1795
BveI ACCTGC 1 cut(s) 820
Cac8I GCNNGC 5 cut(s) 589, 1064, 1753, 1823, 2255
Cfr13I GGNCC 3 cut(s) 628, 1151, 2245
ClaI ATCGAT 1 cut(s) 1199
CseI GACGC 2 cut(s) 1587, 1920
Csp6I GTAC 6 cut(s) 1045, 1675, 2050, 2129, 2824, 2830
CspCI CAANNNNNGTGG 2 cut(s) 1143, 1178
CviQI GTAC 6 cut(s) 1045, 1675, 2050, 2129, 2824, 2830
DdeI CTNAG 6 cut(s) 1369, 1376, 1508, 2068, 2489, 2967
DpnI GATC 8 cut(s) 48, 360, 443, 950, 1374, 1947, 2714, 2780
DpnII GATC 8 cut(s) 46, 358, 441, 948, 1372, 1945, 2712, 2778
DraI TTTAAA 1 cut(s) 2754
EaeI YGGCCR 4 cut(s) 306, 354, 1122, 1193
Eam1104I CTCTTC 5 cut(s) 189, 394, 464, 1988, 2952
EarI CTCTTC 5 cut(s) 189, 394, 464, 1988, 2952
Eco130I CCWWGG 4 cut(s) 162, 573, 2113, 2248
Eco24I GRGCYC 1 cut(s) 184
Eco31I GGTCTC 2 cut(s) 868, 2295
Eco32I GATATC 2 cut(s) 943, 1647
Eco47I GGWCC 2 cut(s) 628, 2245
Eco57I CTGAAG 4 cut(s) 951, 1484, 1511, 2153
Eco72I CACGTG 1 cut(s) 298
EcoO109I RGGNCCY 1 cut(s) 628
EcoRII CCWGG 3 cut(s) 1084, 2328, 2676
EcoRV GATATC 2 cut(s) 943, 1647
EcoT14I CCWWGG 4 cut(s) 162, 573, 2113, 2248
EcoT22I ATGCAT 4 cut(s) 1104, 1561, 1618, 2537
EcoT38I GRGCYC 1 cut(s) 184
ErhI CCWWGG 4 cut(s) 162, 573, 2113, 2248
Esp3I CGTCTC 1 cut(s) 380
FalI AAGNNNNNCTT 6 cut(s) 640, 672, 890, 922, 972, 1004
FaqI GGGAC 4 cut(s) 641, 1335, 2072, 2823
FauI CCCGC 1 cut(s) 2250
FauNDI CATATG 2 cut(s) 2361, 2531
FbaI TGATCA 1 cut(s) 46
Fnu4HI GCNGC 7 cut(s) 264, 405, 786, 2593, 2596, 2628, 2859
FokI GGATG 7 cut(s) 782, 858, 1616, 1625, 2426, 2466, 2529
FriOI GRGCYC 1 cut(s) 184
Fsp4HI GCNGC 7 cut(s) 264, 405, 786, 2593, 2596, 2628, 2859
FspBI CTAG 6 cut(s) 408, 486, 1059, 1752, 2099, 2550
GluI GCNGC 7 cut(s) 264, 405, 786, 2593, 2596, 2628, 2859
GsaI CCCAGC 2 cut(s) 368, 2289
GsuI CTGGAG 3 cut(s) 1274, 2066, 2480
HaeIII GGCC 6 cut(s) 308, 356, 1124, 1152, 1195, 2253
HapII CCGG 5 cut(s) 126, 274, 722, 1381, 2841
HgaI GACGC 2 cut(s) 1587, 1920
HincII GTYRAC 4 cut(s) 61, 1767, 1858, 2175
HindII GTYRAC 4 cut(s) 61, 1767, 1858, 2175
HindIII AAGCTT 3 cut(s) 1053, 1434, 1589
HpaI GTTAAC 1 cut(s) 1767
HpaII CCGG 5 cut(s) 126, 274, 722, 1381, 2841
HphI GGTGA 3 cut(s) 262, 535, 660
Hpy99I CGWCG 1 cut(s) 795
HpyAV CCTTC 7 cut(s) 1211, 1508, 2234, 2569, 2734, 2817, 2897
HpyCH4IV ACGT 4 cut(s) 297, 1271, 2826, 2886
HpyF10VI GCNNNNNNNGC 8 cut(s) 243, 269, 413, 588, 734, 812, 1779, 2855
HpyF3I CTNAG 6 cut(s) 1369, 1376, 1508, 2068, 2489, 2967
HpySE526I ACGT 4 cut(s) 297, 1271, 2826, 2886
Ksp22I TGATCA 1 cut(s) 46
KspAI GTTAAC 1 cut(s) 1767
Kzo9I GATC 8 cut(s) 46, 358, 441, 948, 1372, 1945, 2712, 2778
LguI GCTCTTC 2 cut(s) 189, 394
LmnI GCTCC 5 cut(s) 579, 1485, 2168, 2461, 2555
Lsp1109I GCAGC 6 cut(s) 250, 416, 797, 2579, 2639, 2845
LweI GCATC 7 cut(s) 512, 610, 985, 1089, 1603, 2361, 2404
MaeI CTAG 6 cut(s) 408, 486, 1059, 1752, 2099, 2550
MaeII ACGT 4 cut(s) 297, 1271, 2826, 2886
MaeIII GTNAC 6 cut(s) 199, 1606, 1685, 1694, 1972, 2023
MalI GATC 8 cut(s) 48, 360, 443, 950, 1374, 1947, 2714, 2780
MboI GATC 8 cut(s) 46, 358, 441, 948, 1372, 1945, 2712, 2778
MfeI CAATTG 4 cut(s) 972, 1849, 2645, 3000
MflI RGATCY 4 cut(s) 441, 1372, 1945, 2778
MhlI GDGCHC 5 cut(s) 125, 184, 751, 2672, 2807
MlsI TGGCCA 2 cut(s) 1124, 1195
MluNI TGGCCA 2 cut(s) 1124, 1195
MlyI GAGTC 2 cut(s) 222, 1969
MmeI TCCRAC 3 cut(s) 1581, 1816, 1923
Mox20I TGGCCA 2 cut(s) 1124, 1195
Mph1103I ATGCAT 4 cut(s) 1104, 1561, 1618, 2537
MroXI GAANNNNTTC 2 cut(s) 1629, 2961
MscI TGGCCA 2 cut(s) 1124, 1195
MslI CAYNNNNRTG 1 cut(s) 1988
Msp20I TGGCCA 2 cut(s) 1124, 1195
MspA1I CMGCKG 1 cut(s) 2285
MspCI CTTAAG 2 cut(s) 1592, 2303
MspI CCGG 5 cut(s) 126, 274, 722, 1381, 2841
MspR9I CCNGG 5 cut(s) 274, 1086, 2330, 2678, 2841
MunI CAATTG 4 cut(s) 972, 1849, 2645, 3000
Mva1269I GAATGC 2 cut(s) 591, 1457
MvaI CCWGG 3 cut(s) 1086, 2330, 2678
MwoI GCNNNNNNNGC 8 cut(s) 243, 269, 413, 588, 734, 812, 1779, 2855
NciI CCSGG 2 cut(s) 274, 2841
NcoI CCATGG 3 cut(s) 162, 573, 2248
NdeI CATATG 2 cut(s) 2361, 2531
NdeII GATC 8 cut(s) 46, 358, 441, 948, 1372, 1945, 2712, 2778
NheI GCTAGC 1 cut(s) 1751
NlaIV GGNNCC 6 cut(s) 581, 629, 1947, 2246, 2463, 2557
NmuCI GTSAC 5 cut(s) 199, 1685, 1694, 1972, 2023
NsiI ATGCAT 4 cut(s) 1104, 1561, 1618, 2537
NspI RCATGY 3 cut(s) 260, 1620, 2129
PciI ACATGT 2 cut(s) 256, 2125
PciSI GCTCTTC 2 cut(s) 189, 394
PcsI WCGNNNNNNNCGW 2 cut(s) 1825, 2414
PctI GAATGC 2 cut(s) 591, 1457
PdmI GAANNNNTTC 2 cut(s) 1629, 2961
PfeI GAWTC 8 cut(s) 489, 665, 1089, 1327, 1504, 1839, 2354, 2693
PflMI CCANNNNNTGG 1 cut(s) 1036
PfoI TCCNGGA 1 cut(s) 2839
PkrI GCNGC 7 cut(s) 265, 406, 787, 2594, 2597, 2629, 2860
PleI GAGTC 2 cut(s) 221, 1969
PmaCI CACGTG 1 cut(s) 298
PmlI CACGTG 1 cut(s) 298
PpsI GAGTC 2 cut(s) 221, 1969
Ppu21I YACGTR 2 cut(s) 298, 2827
PpuMI RGGWCCY 1 cut(s) 628
PscI ACATGT 2 cut(s) 256, 2125
PshBI ATTAAT 2 cut(s) 1203, 1533
Psp1406I AACGTT 1 cut(s) 1271
Psp5II RGGWCCY 1 cut(s) 628
Psp6I CCWGG 3 cut(s) 1084, 2328, 2676
PspCI CACGTG 1 cut(s) 298
PspFI CCCAGC 2 cut(s) 364, 2285
PspGI CCWGG 3 cut(s) 1084, 2328, 2676
PspN4I GGNNCC 6 cut(s) 581, 629, 1947, 2246, 2463, 2557
PspPI GGNCC 3 cut(s) 628, 1151, 2245
PspPPI RGGWCCY 1 cut(s) 628
PsuI RGATCY 4 cut(s) 441, 1372, 1945, 2778
PvuII CAGCTG 1 cut(s) 2285
RsaI GTAC 6 cut(s) 1046, 1676, 2051, 2130, 2825, 2831
RsaNI GTAC 6 cut(s) 1045, 1675, 2050, 2129, 2824, 2830
RseI CAYNNNNRTG 1 cut(s) 1988
SapI GCTCTTC 2 cut(s) 189, 394
SatI GCNGC 7 cut(s) 264, 405, 786, 2593, 2596, 2628, 2859
Sau3AI GATC 8 cut(s) 46, 358, 441, 948, 1372, 1945, 2712, 2778
Sau96I GGNCC 3 cut(s) 628, 1151, 2245
SchI GAGTC 2 cut(s) 222, 1969
ScrFI CCNGG 5 cut(s) 274, 1086, 2330, 2678, 2841
SduI GDGCHC 5 cut(s) 125, 184, 751, 2672, 2807
SfaNI GCATC 7 cut(s) 512, 610, 985, 1089, 1603, 2361, 2404
SfcI CTRYAG 3 cut(s) 37, 1304, 2782
SinI GGWCC 2 cut(s) 628, 2245
SmiI ATTTAAAT 1 cut(s) 2754
SmiMI CAYNNNNRTG 1 cut(s) 1988
SmlI CTYRAG 4 cut(s) 331, 871, 1592, 2303
SmoI CTYRAG 4 cut(s) 331, 871, 1592, 2303
SsiI CCGC 4 cut(s) 416, 2257, 2427, 2596
SspI AATATT 2 cut(s) 340, 1207
SspMI CTAG 6 cut(s) 408, 486, 1059, 1752, 2099, 2550
StyD4I CCNGG 5 cut(s) 272, 1084, 2328, 2676, 2839
StyI CCWWGG 4 cut(s) 162, 573, 2113, 2248
SwaI ATTTAAAT 1 cut(s) 2754
TaiI ACGT 4 cut(s) 300, 1274, 2829, 2889
TaqI TCGA 8 cut(s) 790, 945, 951, 1114, 1199, 1920, 2271, 2616
TatI WGTACW 4 cut(s) 1674, 2049, 2128, 2829
TauI GCSGC 1 cut(s) 2598
TfiI GAWTC 8 cut(s) 489, 665, 1089, 1327, 1504, 1839, 2354, 2693
TscAI CASTG 3 cut(s) 357, 1081, 1795
TseFI GTSAC 5 cut(s) 199, 1685, 1694, 1972, 2023
TseI GCWGC 6 cut(s) 263, 404, 785, 2592, 2627, 2858
Tsp45I GTSAC 5 cut(s) 199, 1685, 1694, 1972, 2023
TspGWI ACGGA 5 cut(s) 404, 1625, 1923, 2423, 2552
TspRI CASTG 3 cut(s) 357, 1081, 1795
Van91I CCANNNNNTGG 1 cut(s) 1036
Vha464I CTTAAG 2 cut(s) 1592, 2303
VneI GTGCAC 2 cut(s) 121, 747
VpaK11BI GGWCC 2 cut(s) 628, 2245
VspI ATTAAT 2 cut(s) 1203, 1533
XapI RAATTY 4 cut(s) 140, 1251, 1886, 2447
XbaI TCTAGA 2 cut(s) 485, 2098
XceI RCATGY 3 cut(s) 260, 1620, 2129
XmnI GAANNNNTTC 2 cut(s) 1629, 2961
XspI CTAG 6 cut(s) 408, 486, 1059, 1752, 2099, 2550
Zsp2I ATGCAT 4 cut(s) 1104, 1561, 1618, 2537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.