RLG00000036817

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000085
Physical Location & Seq
Reverse (-)
85109032 .. 85114640
5609 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036817

Sequence Viewer

Length: 2565 bp
ATGGCCTGGTTCTTTCGAAAACCTGTTTCATCTCGAAAAACTCTTGCATCTCGTTCTTCAACAGCTGAACCACCATCTACACCTTCAACAGCTGAATCACCATCTACAGCTCGTCGCTGGAAGTATGACGTGTTTTTGAGTGCCGGGGATGGTGACATTGAAAAGAATATTATATTCGATTTAAGCCATCGGCTGCAAGAGAGGGGAATCACAACATTCATAGATGATTCAGATTTTGTCATAAAACAGATAGATGATTCAAATTTTCGAGTAAGGGATGGTGTTTCTCTCACTCGCGAAATGGGTCATCCACTTCCCACTCTCCTAAATGCAATTGAAGAATCATGGTTGGCAATTGTTGTTCTGTCCCAAAACTATGCTTGCATAAGGTGTCAGGATGAACTTCTAGAGATTTGTCATTTCATGGAGGACGGCCATAGAATTCTTCCAGTTTTTTATAAGGTCGACCCTAGTGATGTAAGAAATCAAAACGGGAGATTTGCAACACCTTTCGTCGACTATAAACGGTTGGAAGCTGCTTTAACAAAAGTGGGCAGTTTGTCTGGGTGGGATGCAAAGAATTACGAGACTGACAAAGATCTTATCGAAGCCATTGCGGAATCTGTTTGCAGTAAACTACAAACTATTCAAATCAGTATCGGAGTTACTGAAATTGAGCCTATAATACCAAATTTGACTGAAGATTTAGAAGCAGTTGAAGCAACAACACAAGCCACCAATGGAAACAGTTCAGATGAACCAGCTCCTCACTGGAAGCATGATGTGTTTTTGAGCTTTAGGGGTGATGACACTCGCAAAGGTTTTCTGTCCCATTTATATCATGAACTGCAAAACACGAAAGTAATTAGAACATTTAAGGATGCTGACCAACTAAAGAAAGGGAAGGATATTTCTCAAAGTCTGCTGATGGCAATTGAAGAATCAAGGTTTGCGGTCATTGTTTTGTCGGAAGACTATGCTTCTTCTGCATGGTGTTTAGGTGAACTTTCAAAGATTTTTCAATGCATGGAAGGCAAGGATACAATTCTGCCAATTTTTTATCATGTGGATCCCTCTGATGTACGATATCAAAAGGGTCAATTTGAAGTGGCCTTTACAAAGCATGAAAGCAAGGGAAGGTATGGTATAGAGAAGGTGAAGCAGTGGAGAGCTGATTTAACAAAAGTGGGAAATCTCTTTGGGTGGGATTCAAAGAACCACAAATCTGAAAGGGAACTCGTTGAAGACATTGTGAAATTTGTGCTCAAAGAAGTACTTCAAGCACCAAGGGGAGATTTTGAAGAATTTGAAGCAACAAGAGAAGCCATCGATGAGGTTAAGAAGATGCTGAAAGATGATAAGATCACTGCCATTGGTGTCTATGGAAGAGGAGGCGTTGGCAAGACAGCCATGGTGAAACATGTCACTGCACAAGCCAAAGGCATTGGGCTTTTTGATCATGTTGTTATGGCTGTTATATCCCGAAACCCTGATTTGCAAAAAATTCAAGGTGCATTGGTAAATCAGCTGGGATTGGAATTGGTGGAGGATGAAGAAATAGAAAGAGCCGGTAGATTGAAGGAGAGGATCATGGCAGGAAAGAGGATCCTTATTATCTTGGATGACATTTGGGAGATAATAGATTTGTCAAGCATTGGAATTCCTGGCTATGATGAGCTTCAAAGATGCAATTCCAAGGTCCTACTCACCACAAGGATGAGGAATATTTGTCATTCTATGGGGTGCCAAGAAGAACACATCATTCCTCTCAATATCCTATCAGAGCAAGATTCTTTGAAGTTATTCATGAAGAAGTCGAGAAAGCCTTTTCATGGACCTACCGATTCTTATGATGAAGAGAGGAACATAGTTAGAGAATGCGCTGGTCTCCCAAGTGTTTTGGAAGAAGCTGCAAGGGCATTTGGACATAAATACATGGAAGAATGGAACGAAGCAGTTTCAAAGGCGTTTAGAGATGATGATGATGATGATGATGAATTGCAAAAAGCAGCTCAAGAACTAAATGCATCCCAACTTGTCCCCACTAAAGATGAGAGAGATACATTTAAAAGGACAGAGTTATGCTATCATACCTTGAGGTTTGATGATGCCAAGTCATTTTTCTTGCTTTGCTGCATGTTTCCAGAAGATTATGATATCCCAATTGAAGACTTGTTTAAGTATGGGATTGGAAAAGGTTTGCTTCGGTATTCAGACACATTGCAAGAAGCCAGAGACCGGGCACATTCTTTGGTCGCATACCTTAAAGCTTCGGGGTTGCTTTTGGATGGCAAGAAGGGATGCATAAGGATGCATTTTGACATCCGAAATGCGGCCATGTCTATTGCGTTGTCTGAAGGTCATGCTTTTTTCGTAAAAGCTGGTTGTGAATTGAAGGATTGGCCAGTGGATGCTCATGAAGGCTACTCAGCAATCTCACTAATGAGGAACAAACTTAACAAGCTTCCTGAAACTCTGCGAAAAGATGGAAGGATATGCATAACCTATAAGAGAGCTGATGCAGGGAAAGGCAAAAGAAAGAGATTCTCAACGGCTGAAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

855

Amino Acids

96.92

Weight (kDa)

5.68

Isoelectric Point (pI)

46.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 106 - 216 5.1e-19 TIR domain
TIR PF01582 259 - 428 2.2e-50 TIR domain
TIR_2 PF13676 262 - 358 5.1e-14 TIR domain
NB-ARC PF00931 442 - 606 1.6e-29 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 459
AccB1I GGYRCC 1 cut(s) 1743
AccI GTMKAC 2 cut(s) 465, 516
AccII CGCG 1 cut(s) 297
AciI CCGC 3 cut(s) 617, 953, 2333
AclWI GGATC 5 cut(s) 1064, 1077, 1595, 1600, 1613
AcoI YGGCCR 3 cut(s) 433, 2334, 2402
AcsI RAATTY 8 cut(s) 262, 441, 691, 1256, 1304, 1503, 1659, 2559
AcuI CTGAAG 2 cut(s) 720, 2376
AfaI GTAC 2 cut(s) 1083, 1275
AfiI CCNNNNNNNGG 3 cut(s) 1832, 2238, 2332
AflIII ACRYGT 2 cut(s) 129, 1420
AjiI CACGTC 1 cut(s) 130
AjnI CCWGG 2 cut(s) 5, 1663
AjuI GAANNNNNNNTTGG 2 cut(s) 2105, 2137
Alw21I GWGCWC 1 cut(s) 1266
Alw26I GTCTC 3 cut(s) 581, 1892, 2229
AlwI GGATC 5 cut(s) 1064, 1077, 1595, 1600, 1613
AoxI GGCC 5 cut(s) 3, 433, 1110, 2334, 2402
ApeKI GCWGC 5 cut(s) 193, 536, 1910, 2009, 2133
ApoI RAATTY 8 cut(s) 262, 441, 691, 1256, 1304, 1503, 1659, 2559
Asp700I GAANNNNTTC 3 cut(s) 748, 1275, 1802
AspLEI GCGC 1 cut(s) 1883
AspS9I GGNCC 2 cut(s) 1699, 1835
AsuC2I CCSGG 2 cut(s) 145, 2240
AsuHPI GGTGA 7 cut(s) 90, 164, 815, 1013, 1168, 1426, 1699
AsuII TTCGAA 1 cut(s) 16
AvaII GGWCC 2 cut(s) 1699, 1835
BaeGI GKGCMC 1 cut(s) 2245
BalI TGGCCA 1 cut(s) 2404
BamHI GGATCC 2 cut(s) 1069, 1605
BanI GGYRCC 1 cut(s) 1743
BbsI GAAGAC 3 cut(s) 978, 1251, 2175
Bbv12I GWGCWC 1 cut(s) 1266
BbvI GCAGC 5 cut(s) 180, 523, 1897, 2021, 2120
BccI CCATC 9 cut(s) 82, 109, 143, 195, 272, 922, 1334, 2282, 2480
BceAI ACGGC 1 cut(s) 448
BcgI CGANNNNNNTGC 2 cut(s) 596, 630
BciT130I CCWGG 2 cut(s) 7, 1665
BciVI GTATCC 1 cut(s) 1033
BclI TGATCA 1 cut(s) 1456
BcnI CCSGG 2 cut(s) 145, 2240
BcoDI GTCTC 3 cut(s) 581, 1892, 2229
BfaI CTAG 2 cut(s) 407, 471
BfmI CTRYAG 1 cut(s) 105
BfuI GTATCC 1 cut(s) 1033
BglII AGATCT 1 cut(s) 598
BisI GCNGC 6 cut(s) 194, 537, 1911, 2010, 2134, 2334
BlsI GCNGC 6 cut(s) 195, 538, 1912, 2011, 2135, 2335
BmcAI AGTACT 1 cut(s) 1275
Bme1390I CCNGG 4 cut(s) 7, 145, 1665, 2240
Bme18I GGWCC 2 cut(s) 1699, 1835
BmgBI CACGTC 1 cut(s) 130
BmgT120I GGNCC 2 cut(s) 1699, 1835
BmiI GGNNCC 3 cut(s) 1071, 1607, 1745
BmrFI CCNGG 4 cut(s) 7, 145, 1665, 2240
BpiI GAAGAC 3 cut(s) 978, 1251, 2175
Bpu14I TTCGAA 1 cut(s) 16
BpuEI CTTGAG 2 cut(s) 1998, 2116
BpuMI CCSGG 2 cut(s) 145, 2240
Bsa29I ATCGAT 1 cut(s) 1329
BsaI GGTCTC 2 cut(s) 1892, 2229
BsaJI CCNNGG 4 cut(s) 144, 1286, 1410, 1695
Bsc4I CCNNNNNNNGG 3 cut(s) 1832, 2238, 2332
Bse118I RCCGGY 1 cut(s) 1568
Bse1I ACTGG 3 cut(s) 449, 776, 2405
Bse3DI GCAATG 2 cut(s) 612, 2219
BseBI CCWGG 2 cut(s) 7, 1665
BseCI ATCGAT 1 cut(s) 1329
BseDI CCNNGG 4 cut(s) 144, 1286, 1410, 1695
BseLI CCNNNNNNNGG 3 cut(s) 1832, 2238, 2332
BseMI GCAATG 2 cut(s) 612, 2219
BseMII CTCAG 1 cut(s) 2442
BseNI ACTGG 3 cut(s) 449, 776, 2405
BseRI GAGGAG 2 cut(s) 756, 1404
BseSI GKGCMC 1 cut(s) 2245
BseXI GCAGC 5 cut(s) 180, 523, 1897, 2021, 2120
BseYI CCCAGC 1 cut(s) 1528
BsgI GTGCAG 1 cut(s) 1413
Bsh1236I CGCG 1 cut(s) 297
BshFI GGCC 5 cut(s) 5, 435, 1112, 2336, 2404
BshNI GGYRCC 1 cut(s) 1743
BshVI ATCGAT 1 cut(s) 1329
BsiHKAI GWGCWC 1 cut(s) 1266
BsiSI CCGG 3 cut(s) 144, 1569, 2239
BslFI GGGAC 3 cut(s) 352, 814, 2024
BslI CCNNNNNNNGG 3 cut(s) 1832, 2238, 2332
BsmAI GTCTC 3 cut(s) 581, 1892, 2229
BsmFI GGGAC 3 cut(s) 352, 814, 2024
BsmI GAATGC 1 cut(s) 1883
BsnI GGCC 5 cut(s) 5, 435, 1112, 2336, 2404
Bso31I GGTCTC 2 cut(s) 1892, 2229
Bsp119I TTCGAA 1 cut(s) 16
Bsp1286I GDGCHC 2 cut(s) 1266, 2245
Bsp143I GATC 6 cut(s) 598, 1069, 1362, 1456, 1587, 1605
Bsp19I CCATGG 1 cut(s) 1410
Bsp68I TCGCGA 1 cut(s) 297
BspACI CCGC 3 cut(s) 617, 953, 2333
BspANI GGCC 5 cut(s) 5, 435, 1112, 2336, 2404
BspCNI CTCAG 1 cut(s) 2441
BspDI ATCGAT 1 cut(s) 1329
BspFNI CGCG 1 cut(s) 297
BspHI TCATGA 3 cut(s) 841, 1806, 2416
BspLI GGNNCC 3 cut(s) 1071, 1607, 1745
BspPI GGATC 5 cut(s) 1064, 1077, 1595, 1600, 1613
BspT104I TTCGAA 1 cut(s) 16
BspT107I GGYRCC 1 cut(s) 1743
BspTNI GGTCTC 2 cut(s) 1892, 2229
BsrDI GCAATG 2 cut(s) 612, 2219
BsrFI RCCGGY 1 cut(s) 1568
BsrI ACTGG 3 cut(s) 449, 776, 2405
BssAI RCCGGY 1 cut(s) 1568
BssECI CCNNGG 4 cut(s) 144, 1286, 1410, 1695
BssMI GATC 6 cut(s) 598, 1069, 1362, 1456, 1587, 1605
BssT1I CCWWGG 3 cut(s) 1286, 1410, 1695
Bst2UI CCWGG 2 cut(s) 7, 1665
Bst4CI ACNGT 2 cut(s) 528, 749
Bst6I CTCTTC 2 cut(s) 1381, 1851
BstBI TTCGAA 1 cut(s) 16
BstC8I GCNNGC 1 cut(s) 382
BstDEI CTNAG 1 cut(s) 2428
BstDSI CCRYGG 1 cut(s) 1410
BstFNI CGCG 1 cut(s) 297
BstHHI GCGC 1 cut(s) 1883
BstKTI GATC 6 cut(s) 601, 1072, 1365, 1459, 1590, 1608
BstMAI GTCTC 3 cut(s) 581, 1892, 2229
BstMBI GATC 6 cut(s) 598, 1069, 1362, 1456, 1587, 1605
BstMWI GCNNNNNNNGC 4 cut(s) 719, 986, 1032, 1916
BstNI CCWGG 2 cut(s) 7, 1665
BstNSI RCATGY 2 cut(s) 1424, 2140
BstSCI CCNGG 4 cut(s) 5, 143, 1663, 2238
BstSFI CTRYAG 1 cut(s) 105
BstSLI GKGCMC 1 cut(s) 2245
BstUI CGCG 1 cut(s) 297
BstV1I GCAGC 5 cut(s) 180, 523, 1897, 2021, 2120
BstV2I GAAGAC 3 cut(s) 978, 1251, 2175
BstX2I RGATCY 3 cut(s) 598, 1069, 1605
BstYI RGATCY 3 cut(s) 598, 1069, 1605
Bsu15I ATCGAT 1 cut(s) 1329
BsuI GTATCC 1 cut(s) 1033
BsuRI GGCC 5 cut(s) 5, 435, 1112, 2336, 2404
BsuTUI ATCGAT 1 cut(s) 1329
BtgI CCRYGG 1 cut(s) 1410
BtrI CACGTC 1 cut(s) 130
BtsI GCAGTG 3 cut(s) 1169, 1365, 1425
BtsIMutI CAGTG 5 cut(s) 769, 1169, 1365, 1425, 2412
BtuMI TCGCGA 1 cut(s) 297
Cac8I GCNNGC 1 cut(s) 382
CciI TCATGA 3 cut(s) 841, 1806, 2416
CfoI GCGC 1 cut(s) 1883
Cfr10I RCCGGY 1 cut(s) 1568
Cfr13I GGNCC 2 cut(s) 1699, 1835
ClaI ATCGAT 1 cut(s) 1329
Csp6I GTAC 2 cut(s) 1082, 1274
CviQI GTAC 2 cut(s) 1082, 1274
DdeI CTNAG 1 cut(s) 2428
DpnI GATC 6 cut(s) 600, 1071, 1364, 1458, 1589, 1607
DpnII GATC 6 cut(s) 598, 1069, 1362, 1456, 1587, 1605
DraI TTTAAA 1 cut(s) 2068
EaeI YGGCCR 3 cut(s) 433, 2334, 2402
Eam1104I CTCTTC 2 cut(s) 1381, 1851
EarI CTCTTC 2 cut(s) 1381, 1851
Eco130I CCWWGG 3 cut(s) 1286, 1410, 1695
Eco31I GGTCTC 2 cut(s) 1892, 2229
Eco32I GATATC 2 cut(s) 1088, 2158
Eco47I GGWCC 2 cut(s) 1699, 1835
Eco57I CTGAAG 2 cut(s) 720, 2376
EcoO109I RGGNCCY 1 cut(s) 1699
EcoRI GAATTC 2 cut(s) 441, 1659
EcoRII CCWGG 2 cut(s) 5, 1663
EcoRV GATATC 2 cut(s) 1088, 2158
EcoT14I CCWWGG 3 cut(s) 1286, 1410, 1695
EcoT22I ATGCAT 5 cut(s) 1028, 2029, 2306, 2316, 2501
ErhI CCWWGG 3 cut(s) 1286, 1410, 1695
FalI AAGNNNNNCTT 4 cut(s) 1260, 1292, 2187, 2219
FaqI GGGAC 3 cut(s) 352, 814, 2024
FbaI TGATCA 1 cut(s) 1456
FblI GTMKAC 2 cut(s) 465, 516
Fnu4HI GCNGC 6 cut(s) 194, 537, 1911, 2010, 2134, 2334
Fsp4HI GCNGC 6 cut(s) 194, 537, 1911, 2010, 2134, 2334
FspBI CTAG 2 cut(s) 407, 471
GlaI GCGC 1 cut(s) 1882
GluI GCNGC 6 cut(s) 194, 537, 1911, 2010, 2134, 2334
GsaI CCCAGC 1 cut(s) 1532
HaeIII GGCC 5 cut(s) 5, 435, 1112, 2336, 2404
HapII CCGG 3 cut(s) 144, 1569, 2239
HhaI GCGC 1 cut(s) 1883
Hin6I GCGC 1 cut(s) 1881
HinP1I GCGC 1 cut(s) 1881
HincII GTYRAC 2 cut(s) 466, 517
HindII GTYRAC 2 cut(s) 466, 517
HindIII AAGCTT 2 cut(s) 2268, 2462
HpaII CCGG 3 cut(s) 144, 1569, 2239
HphI GGTGA 7 cut(s) 90, 164, 815, 1013, 1168, 1426, 1699
Hpy166II GTNNAC 4 cut(s) 466, 517, 635, 1004
Hpy8I GTNNAC 4 cut(s) 466, 517, 635, 1004
Hpy99I CGWCG 2 cut(s) 117, 518
HpyCH4III ACNGT 2 cut(s) 528, 749
HpyCH4IV ACGT 1 cut(s) 129
HpyF10VI GCNNNNNNNGC 4 cut(s) 719, 986, 1032, 1916
HpyF3I CTNAG 1 cut(s) 2428
HpySE526I ACGT 1 cut(s) 129
HspAI GCGC 1 cut(s) 1881
Ksp22I TGATCA 1 cut(s) 1456
Kzo9I GATC 6 cut(s) 598, 1069, 1362, 1456, 1587, 1605
LmnI GCTCC 1 cut(s) 769
Lsp1109I GCAGC 5 cut(s) 180, 523, 1897, 2021, 2120
MaeI CTAG 2 cut(s) 407, 471
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 3 cut(s) 152, 664, 1423
MalI GATC 6 cut(s) 600, 1071, 1364, 1458, 1589, 1607
MboI GATC 6 cut(s) 598, 1069, 1362, 1456, 1587, 1605
MfeI CAATTG 4 cut(s) 333, 354, 933, 2163
MflI RGATCY 3 cut(s) 598, 1069, 1605
MhlI GDGCHC 2 cut(s) 1266, 2245
MlsI TGGCCA 1 cut(s) 2404
MluNI TGGCCA 1 cut(s) 2404
MmeI TCCRAC 2 cut(s) 510, 948
Mox20I TGGCCA 1 cut(s) 2404
Mph1103I ATGCAT 5 cut(s) 1028, 2029, 2306, 2316, 2501
MroXI GAANNNNTTC 3 cut(s) 748, 1275, 1802
MscI TGGCCA 1 cut(s) 2404
MseI TTAA 9 cut(s) 182, 542, 876, 1178, 1338, 2067, 2178, 2265, 2457
MslI CAYNNNNRTG 2 cut(s) 1715, 2309
Msp20I TGGCCA 1 cut(s) 2404
MspA1I CMGCKG 3 cut(s) 65, 92, 1528
MspI CCGG 3 cut(s) 144, 1569, 2239
MspR9I CCNGG 4 cut(s) 7, 145, 1665, 2240
MunI CAATTG 4 cut(s) 333, 354, 933, 2163
Mva1269I GAATGC 1 cut(s) 1883
MvaI CCWGG 2 cut(s) 7, 1665
MvnI CGCG 1 cut(s) 297
MwoI GCNNNNNNNGC 4 cut(s) 719, 986, 1032, 1916
NciI CCSGG 2 cut(s) 145, 2240
NcoI CCATGG 1 cut(s) 1410
NdeII GATC 6 cut(s) 598, 1069, 1362, 1456, 1587, 1605
NlaIV GGNNCC 3 cut(s) 1071, 1607, 1745
NmuCI GTSAC 2 cut(s) 152, 1423
NruI TCGCGA 1 cut(s) 297
NsiI ATGCAT 5 cut(s) 1028, 2029, 2306, 2316, 2501
NspI RCATGY 2 cut(s) 1424, 2140
NspV TTCGAA 1 cut(s) 16
PagI TCATGA 3 cut(s) 841, 1806, 2416
PciI ACATGT 1 cut(s) 1420
PctI GAATGC 1 cut(s) 1883
PdmI GAANNNNTTC 3 cut(s) 748, 1275, 1802
PkrI GCNGC 6 cut(s) 195, 538, 1912, 2011, 2135, 2335
PpuMI RGGWCCY 1 cut(s) 1699
PscI ACATGT 1 cut(s) 1420
PsiI TTATAA 1 cut(s) 459
Psp5II RGGWCCY 1 cut(s) 1699
Psp6I CCWGG 2 cut(s) 5, 1663
PspFI CCCAGC 1 cut(s) 1528
PspGI CCWGG 2 cut(s) 5, 1663
PspN4I GGNNCC 3 cut(s) 1071, 1607, 1745
PspPI GGNCC 2 cut(s) 1699, 1835
PspPPI RGGWCCY 1 cut(s) 1699
PsuI RGATCY 3 cut(s) 598, 1069, 1605
PvuII CAGCTG 3 cut(s) 65, 92, 1528
RruI TCGCGA 1 cut(s) 297
RsaI GTAC 2 cut(s) 1083, 1275
RsaNI GTAC 2 cut(s) 1082, 1274
RseI CAYNNNNRTG 2 cut(s) 1715, 2309
SalI GTCGAC 2 cut(s) 464, 515
SaqAI TTAA 9 cut(s) 182, 542, 876, 1178, 1338, 2067, 2178, 2265, 2457
SatI GCNGC 6 cut(s) 194, 537, 1911, 2010, 2134, 2334
Sau3AI GATC 6 cut(s) 598, 1069, 1362, 1456, 1587, 1605
Sau96I GGNCC 2 cut(s) 1699, 1835
ScaI AGTACT 1 cut(s) 1275
ScrFI CCNGG 4 cut(s) 7, 145, 1665, 2240
SduI GDGCHC 2 cut(s) 1266, 2245
SfcI CTRYAG 1 cut(s) 105
SfuI TTCGAA 1 cut(s) 16
SinI GGWCC 2 cut(s) 1699, 1835
SmiMI CAYNNNNRTG 2 cut(s) 1715, 2309
SmlI CTYRAG 2 cut(s) 2013, 2095
SmoI CTYRAG 2 cut(s) 2013, 2095
SsiI CCGC 3 cut(s) 617, 953, 2333
SspI AATATT 2 cut(s) 169, 1726
SspMI CTAG 2 cut(s) 407, 471
StyD4I CCNGG 4 cut(s) 5, 143, 1663, 2238
StyI CCWWGG 3 cut(s) 1286, 1410, 1695
TaaI ACNGT 2 cut(s) 528, 749
TaiI ACGT 1 cut(s) 132
TaqI TCGA 9 cut(s) 16, 34, 177, 268, 465, 516, 606, 1329, 1817
TatI WGTACW 1 cut(s) 1273
TauI GCSGC 1 cut(s) 2336
Tru1I TTAA 9 cut(s) 182, 542, 876, 1178, 1338, 2067, 2178, 2265, 2457
Tru9I TTAA 9 cut(s) 182, 542, 876, 1178, 1338, 2067, 2178, 2265, 2457
TscAI CASTG 5 cut(s) 776, 1169, 1372, 1432, 2412
TseFI GTSAC 2 cut(s) 152, 1423
TseI GCWGC 5 cut(s) 193, 536, 1910, 2009, 2133
Tsp45I GTSAC 2 cut(s) 152, 1423
TspRI CASTG 5 cut(s) 776, 1169, 1372, 1432, 2412
VpaK11BI GGWCC 2 cut(s) 1699, 1835
XapI RAATTY 8 cut(s) 262, 441, 691, 1256, 1304, 1503, 1659, 2559
XbaI TCTAGA 1 cut(s) 406
XceI RCATGY 2 cut(s) 1424, 2140
XcmI CCANNNNNNNNNTGG 1 cut(s) 768
XmiI GTMKAC 2 cut(s) 465, 516
XmnI GAANNNNTTC 3 cut(s) 748, 1275, 1802
XspI CTAG 2 cut(s) 407, 471
ZrmI AGTACT 1 cut(s) 1275
Zsp2I ATGCAT 5 cut(s) 1028, 2029, 2306, 2316, 2501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.