Rw1G005530

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000085
Physical Location & Seq
Reverse (-)
11070264 .. 11075734
5471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G005530.1

Sequence Viewer

Length: 3471 bp
ATGGCCTCGAGCACCCAAACAGCATCCTCATCTCATCCTTCATCATCTGCTCCTCGGTGGAAGTACGATGTTTTTCTGAGTTTCCGGGGTGAAGATACTCGCAGGACTATTATCCCCCTTTTATACCGGGAATTGCAAAACAGAGAAATTGAAACATTCATGGATGACACACGGCTTAGCAGAGGGACGTCTATTGCTCCGAGTCTGCTAACAGCAATCCAAGAATCCAGGTCAGCGATCGTTGTTCTCTCACCAAACTATGCGTATTCTATATGGTGTTTGGACGAATTGACAATGGTCGTTCAGTGCATGGAAGATAAAAGCACAACAGTCATCCCAATCTTTTATGATGTGGTTCCCTCTGAGGTGCGACATCAGAAAGGGAGCTTTGCAAGAGCCTTCACCAAGCACCAAGAAAGGCTTGCGCAAGACGATGACTACCCAAATAAGTTGAACCAGTGGCGAGTTGCTTTAACAAAAGTTGCCGGTCTCTCTGGCTTGGAATCAAACAAATATAGTTCTGACTTAGAACTTGTCGAAGACATTGTGAAAATCGTGTGCAGTAAAGTACGACCTATTGATGTTGAGGGGGCATTGTCGTCTACCGGAGATTTTGTAGTATTTGAAGCAACGAGACGAGCCATGGACAAGGTAATGAGGGCACTTCAAGATGATGAGATTACTGCCATTGGGGTCTTTGGTATGGGAGGCGTTGGCAAGACAACTATGGTGAAACATGTCGGTGCACAAGCCCGGAAAATTGGGCTTTTTAATCATGTGATCATAGCTGTCATATCCCAAACACCTAATTTGAGAAATATTCAAGGCACACTGGCTGATCTGCTTGGTTTAAAGTTGCAGGAGGAGACAGATATTGGAAGAGCCCGTAGACTAGTGGAGAAGATATTAAGAGGATATAAGATCCTTATAATCTTGGATGACATTTGGGAGAGAATAGCTTTGTCTAGCATTGGAATACCGAGCCATGATGAGCTTCAAAAGTGCAGTTCCAAAATCATATTCACCACAAGGAGATCGAATGTTTGCCATACCATGGAGAGCCAAGAAAACATTCCTCTCCAAGTCTTATCAGAAAACGATGCTTGGAACCTATTTATGAAGAAAGCAAGAAAGCCTTTTCATGAATCGAGTTACTATGTTGTAGGGCGGCGAGTAGCAACCGAATGTGCTGGTCTCCCAGTTGCATTGGTAGCAGTTGCCAGGGCACTTGGAGATAAAGACTTAGATGATTGGAAGGAAGCAGCTCGACGACTAAAGGCTGCTCAACCTGCCAACTATGAAGATGAGGGAGCTGTGTTCAGATGTATAAAGTTAAGCTATGATTACTTGAGACCTGATGACAACAACATTGCCAAGTCATTCTTCTTGCTTTGCTGTCTATTCCCTGAAGATTTTGATATCAAGGTGGAAGACTTGTTGATGTATGCAATTGGGAAAGGAATATTTCAAGATACCCACGTAATAGAAGAAGCCAGAGCCAAAGCCCATTTGGTGGTTAAGTACCTGAAAGCTTCTAGCTTGCTTTTGGACAGTGCATACCACGGATTGGTGAAGATGCATGATGTCATTCGAGATGTGGCCATAGCAATTTCATCCTCGGAAGATGGCCCAGGGTTTTTGGTTAGAGCTGGTTGTGAATTAAGGGATTGGCCAGCGATTAATATTGCACATGATGGCTACTCTGCAATCTCACTAATGAGGAATGAAATTCACGAGCTTCCCAAAAAGTTGGTATGTTCAAAACTCCAGATTTTATTACTACAGAGCAATAGTATAAATGAGATTCCAAAACCTTTCTTTCAAAGTCCCAATGAATTGAGGGTCTTAGATCTCAGCGAGACTAGTATTTCGTGTCTACCCTCATCATCAAGTCTTCTAACCAACCTCCAAGCTTTGTATTTAGATGAATGCAAGAGAATAGTTGATATATCCATGCTCGGAAAACTGAAGAACCTTCAGATTCTTAGTATGAAGAACCTTGACATTATTAATACGGGAGGAACTCGTCAAAAAGCATTGCCGAAAGAAATAGGACGCTTAACCAAGCTTAAGATGTTGGATGTTACGGATGCATGTTTTGAAGCAGTTACATCTAATGTGATATCAAACTTGAATGGATTAGAAGAATTGTACATGAAATGTGACTTTTCTGACTGGGGCAGTAGAATAGTTGAGGGCAGCAGTTTATGTTCTTCTGTTTTTGGCACTGCTAAGAATGCTAGCTTTGATGAGTTAACAAGCTTATCACGCTTAAAGAGTTTGGAAGTTCACATATCGGATGTAGAATGCTTGCCCCGAAAAGTTAGATTCAATCCCAATTGGGCCAACTTTGATATATGTATTAGCAGAAAAGAATTTATGAAACAGTCCTACTACGGACGCTTTTTCGGGAAGAAACCAACTGTCATGGATCCACGTCAACGTCTCTTTCGGTATGTGACTCTAAACACAAGCACAGAGAGTTTACCAGAATGGTTTCTCAAGGTCGTGACAGCGAAAGCAGAGATACTGGAGTACATAGGGTACAGTGGATTAGAGGACATTCTTGTGGAACACGACTTTGGGAAATTACATGTGCTGAAGTGTCTTGCTGTAATTGAGTGCCGTATGTATGGAGAGTTGATGAACTCAGGAAGAAGGGTTCTAAATAAACCTTTGTTTGAGAGCTTGATAGAACTGTATCTGGAAGGGGTCCATGGCCTGTGCCGGTTATGTATTGGTGAGTTACCACCTGGGTCTCTGTGCAATCTTAAGTTGTTAAATGTGAGAAATTGCCTGGAATTGGTGGGTGCACTTTTACAACCAAATTTGTTGCGAAGGCTACAAAATCTGGAAATACTACATTGTGAGTACACGGAGGATGCGAGAGAAACCAAATTGGAATCTGTGTTTGATTTTGCATGGATGGAAGATGAAAAAATTGCCTTACCAAAGTTGAGAGAGATAACATTATGTCATCTTGTGAACCTAAGAAGCATATGCAGTGGTCGTCCTCCCAATGAAGTCTTCCGTAATCTTAAAAGTTTGGCAATTCGTGGCTGCCATGGGATGTTTAGTCTGTTGCCGTCCAATGTAGCTCAATCTCTTTTCCAATTGGAACTCCTTTGGGTGGATGAGTGCTCTTTCTTGGAAACAGTTATCGAATCAAGCTGGGGAACAGAGATTGACCTTCCAAAATTGAAGGAAATATATTTGAATAGTCTTCCACATCTCCGATCGATCTCTAGAAGCACTGTTGTGTGCCCTTTATTGGAACACTTGTATGTGTACAATTGCCCCCTACTTTCAAGCCTTGCTGCTTCTGACTTCCAAAGCAAGAACCACGTCCAAGCCAATAATGAAGGACATTTTGCAATTATATTTGAAAGATTGTTCCACCGCCAATTCAATCCAGAAGAGGTTCCTCAGTTCTTCTCCAAAAAGGAAAGTGATTTCTCAATTGGCTCTTAA

Protein Analysis

1156

Amino Acids

130.55

Weight (kDa)

6.34

Isoelectric Point (pI)

49.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 21 - 194 7.3e-49 TIR domain
TIR_2 PF13676 24 - 120 1.8e-14 TIR domain
NB-ARC PF00931 214 - 376 1.8e-30 NB-ARC domain
LRR_14 PF23598 588 - 667 2.1e-06 Leucine-rich repeat region
LRR_8 PF13855 588 - 644 6e-09 Leucine rich repeat
LRR_14 PF23598 674 - 953 2e-06 Leucine-rich repeat region
LRR_RPS2 PF23247 908 - 1047 3.7e-19 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1001 - 1105 3.9e-13 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 929
AatII GACGTC 1 cut(s) 191
Acc16I TGCGCA 1 cut(s) 426
Acc36I ACCTGC 1 cut(s) 1297
AccB7I CCANNNNNTGG 3 cut(s) 1054, 1513, 1567
AccI GTMKAC 3 cut(s) 602, 889, 1876
AciI CCGC 2 cut(s) 1168, 3400
AclWI GGATC 3 cut(s) 916, 2426, 2439
AcoI YGGCCR 2 cut(s) 1599, 1670
AcsI RAATTY 3 cut(s) 1728, 2375, 2827
AcuI CTGAAG 4 cut(s) 1428, 1961, 1988, 2621
AcyI GRCGYC 1 cut(s) 188
AfaI GTAC 8 cut(s) 65, 570, 1523, 2153, 2537, 2546, 2873, 3290
AfiI CCNNNNNNNGG 6 cut(s) 1054, 1513, 1567, 2803, 3130, 3271
AflII CTTAAG 2 cut(s) 2069, 2771
AflIII ACRYGT 2 cut(s) 736, 2593
AhlI ACTAGT 2 cut(s) 892, 1862
AjiI CACGTC 2 cut(s) 2438, 3346
AjnI CCWGG 5 cut(s) 227, 1220, 1630, 2752, 2796
AleI CACNNNNGTG 1 cut(s) 3257
AloI GAACNNNNNNTCC 2 cut(s) 2646, 2678
Alw21I GWGCWC 4 cut(s) 14, 748, 2815, 3143
Alw26I GTCTC 8 cut(s) 494, 628, 860, 1199, 1345, 1853, 2450, 2763
Alw44I GTGCAC 2 cut(s) 744, 2811
AlwI GGATC 3 cut(s) 916, 2426, 2439
Ama87I CYCGRG 1 cut(s) 7
AoxI GGCC 6 cut(s) 3, 1599, 1627, 1670, 2343, 2719
ApaLI GTGCAC 2 cut(s) 744, 2811
ApeKI GCWGC 5 cut(s) 1262, 1280, 2199, 3060, 3317
ApoI RAATTY 3 cut(s) 1728, 2375, 2827
ArsI GACNNNNNNTTYG 2 cut(s) 1852, 1884
AseI ATTAAT 2 cut(s) 1680, 2010
Asp700I GAANNNNTTC 3 cut(s) 1071, 2496, 3420
AspLEI GCGC 1 cut(s) 427
AspS9I GGNCC 3 cut(s) 1628, 2343, 2713
AsuC2I CCSGG 3 cut(s) 86, 128, 754
AsuHPI GGTGA 7 cut(s) 101, 243, 394, 742, 1015, 1582, 2753
AsuNHI GCTAGC 1 cut(s) 2240
AvaI CYCGRG 1 cut(s) 7
AvaII GGWCC 1 cut(s) 2713
BaeGI GKGCMC 5 cut(s) 664, 748, 1228, 2815, 3266
BaeI ACNNNNGTAYC 4 cut(s) 1464, 1497, 2519, 2552
BalI TGGCCA 2 cut(s) 1601, 1672
BamHI GGATCC 1 cut(s) 2431
BanII GRGCYC 1 cut(s) 886
BauI CACGAG 1 cut(s) 1733
BbsI GAAGAC 5 cut(s) 546, 1437, 1886, 3019, 3215
Bbv12I GWGCWC 4 cut(s) 14, 748, 2815, 3143
BbvI GCAGC 5 cut(s) 1267, 1274, 2211, 3047, 3304
BccI CCATC 3 cut(s) 1619, 1688, 2920
BceAI ACGGC 3 cut(s) 188, 2610, 3070
BciT130I CCWGG 5 cut(s) 229, 1222, 1632, 2754, 2798
BclI TGATCA 1 cut(s) 780
BcnI CCSGG 3 cut(s) 86, 128, 754
BcoDI GTCTC 8 cut(s) 494, 628, 860, 1199, 1345, 1853, 2450, 2763
BcuI ACTAGT 2 cut(s) 892, 1862
BfaI CTAG 6 cut(s) 893, 966, 1536, 1863, 2241, 3246
BfmI CTRYAG 1 cut(s) 1781
BfrI CTTAAG 2 cut(s) 2069, 2771
BfuAI ACCTGC 1 cut(s) 1297
BglII AGATCT 1 cut(s) 1849
BisI GCNGC 6 cut(s) 1169, 1263, 1281, 2200, 3061, 3318
BlpI GCTNAGC 1 cut(s) 176
BlsI GCNGC 6 cut(s) 1170, 1264, 1282, 2201, 3062, 3319
Bme1390I CCNGG 8 cut(s) 86, 128, 229, 754, 1222, 1632, 2754, 2798
Bme18I GGWCC 1 cut(s) 2713
BmeT110I CYCGRG 1 cut(s) 7
BmgBI CACGTC 2 cut(s) 2438, 3346
BmgT120I GGNCC 3 cut(s) 1628, 2343, 2713
BmiI GGNNCC 5 cut(s) 357, 1109, 2433, 2714, 3423
BmrFI CCNGG 8 cut(s) 86, 128, 229, 754, 1222, 1632, 2754, 2798
BmrI ACTGGG 2 cut(s) 1193, 2185
BmsI GCATC 5 cut(s) 32, 1090, 1566, 2080, 2872
BmtI GCTAGC 1 cut(s) 2244
BmuI ACTGGG 2 cut(s) 1193, 2185
BoxI GACNNNNGTC 1 cut(s) 296
BpiI GAAGAC 5 cut(s) 546, 1437, 1886, 3019, 3215
BpmI CTGGAG 2 cut(s) 1751, 2552
Bpu1102I GCTNAGC 1 cut(s) 176
BpuEI CTTGAG 2 cut(s) 1369, 2486
BpuMI CCSGG 3 cut(s) 86, 128, 754
Bsa29I ATCGAT 1 cut(s) 3239
BsaAI YACGTR 1 cut(s) 1480
BsaHI GRCGYC 1 cut(s) 188
BsaI GGTCTC 4 cut(s) 494, 1199, 1345, 2763
BsaWI WCCGGW 1 cut(s) 605
BsaXI ACNNNNNCTCC 2 cut(s) 600, 630
Bsc4I CCNNNNNNNGG 6 cut(s) 1054, 1513, 1567, 2803, 3130, 3271
Bse118I RCCGGY 2 cut(s) 485, 2727
Bse1I ACTGG 5 cut(s) 457, 837, 1199, 2180, 2535
Bse3DI GCAATG 2 cut(s) 1368, 2036
BseBI CCWGG 5 cut(s) 229, 1222, 1632, 2754, 2798
BseCI ATCGAT 1 cut(s) 3239
BseLI CCNNNNNNNGG 6 cut(s) 1054, 1513, 1567, 2803, 3130, 3271
BseMI GCAATG 2 cut(s) 1368, 2036
BseMII CTCAG 5 cut(s) 68, 354, 1867, 2664, 3440
BseNI ACTGG 5 cut(s) 457, 837, 1199, 2180, 2535
BseRI GAGGAG 2 cut(s) 42, 878
BseSI GKGCMC 5 cut(s) 664, 748, 1228, 2815, 3266
BseXI GCAGC 5 cut(s) 1267, 1274, 2211, 3047, 3304
BseYI CCCAGC 1 cut(s) 3171
BsgI GTGCAG 2 cut(s) 580, 1024
Bsh1285I CGRYCG 2 cut(s) 240, 3239
BshFI GGCC 6 cut(s) 5, 1601, 1629, 1672, 2345, 2721
BshVI ATCGAT 1 cut(s) 3239
BsiEI CGRYCG 2 cut(s) 240, 3239
BsiHKAI GWGCWC 4 cut(s) 14, 748, 2815, 3143
BsiHKCI CYCGRG 1 cut(s) 7
BsiSI CCGG 6 cut(s) 85, 127, 486, 606, 754, 2728
BslFI GGGAC 2 cut(s) 199, 1812
BslI CCNNNNNNNGG 6 cut(s) 1054, 1513, 1567, 2803, 3130, 3271
BsmAI GTCTC 8 cut(s) 494, 628, 860, 1199, 1345, 1853, 2450, 2763
BsmBI CGTCTC 2 cut(s) 628, 2450
BsmFI GGGAC 2 cut(s) 199, 1812
BsmI GAATGC 3 cut(s) 1934, 2242, 2312
BsnI GGCC 6 cut(s) 5, 1601, 1629, 1672, 2345, 2721
Bso31I GGTCTC 4 cut(s) 494, 1199, 1345, 2763
BsoBI CYCGRG 1 cut(s) 7
Bsp1286I GDGCHC 8 cut(s) 14, 664, 748, 886, 1228, 2815, 3143, 3266
Bsp1407I TGTACA 2 cut(s) 2151, 3288
Bsp143I GATC 9 cut(s) 237, 780, 838, 921, 1034, 1849, 2431, 3236, 3240
Bsp1720I GCTNAGC 1 cut(s) 176
Bsp19I CCATGG 4 cut(s) 642, 1053, 2716, 3064
BspACI CCGC 2 cut(s) 1168, 3400
BspANI GGCC 6 cut(s) 5, 1601, 1629, 1672, 2345, 2721
BspCNI CTCAG 5 cut(s) 69, 355, 1866, 2663, 3439
BspDI ATCGAT 1 cut(s) 3239
BspHI TCATGA 1 cut(s) 1141
BspLI GGNNCC 5 cut(s) 357, 1109, 2433, 2714, 3423
BspMI ACCTGC 1 cut(s) 1297
BspOI GCTAGC 1 cut(s) 2244
BspPI GGATC 3 cut(s) 916, 2426, 2439
BspQI GCTCTTC 1 cut(s) 874
BspTI CTTAAG 2 cut(s) 2069, 2771
BspTNI GGTCTC 4 cut(s) 494, 1199, 1345, 2763
BsrDI GCAATG 2 cut(s) 1368, 2036
BsrFI RCCGGY 2 cut(s) 485, 2727
BsrGI TGTACA 2 cut(s) 2151, 3288
BsrI ACTGG 5 cut(s) 457, 837, 1199, 2180, 2535
BssAI RCCGGY 2 cut(s) 485, 2727
BssMI GATC 9 cut(s) 237, 780, 838, 921, 1034, 1849, 2431, 3236, 3240
BssNI GRCGYC 1 cut(s) 188
BssSI CACGAG 1 cut(s) 1733
BssT1I CCWWGG 4 cut(s) 642, 1053, 2716, 3064
Bst2BI CACGAG 1 cut(s) 1733
Bst2UI CCWGG 5 cut(s) 229, 1222, 1632, 2754, 2798
Bst4CI ACNGT 8 cut(s) 331, 1553, 2388, 2425, 2549, 2700, 3157, 3256
Bst6I CTCTTC 2 cut(s) 874, 3411
BstACI GRCGYC 1 cut(s) 188
BstAFI CTTAAG 2 cut(s) 2069, 2771
BstAUI TGTACA 2 cut(s) 2151, 3288
BstBAI YACGTR 1 cut(s) 1480
BstC8I GCNNGC 5 cut(s) 423, 1541, 1674, 2242, 2312
BstDSI CCRYGG 5 cut(s) 642, 1053, 1561, 2716, 3064
BstHHI GCGC 1 cut(s) 427
BstKTI GATC 9 cut(s) 240, 783, 841, 924, 1037, 1852, 2434, 3239, 3243
BstMAI GTCTC 8 cut(s) 494, 628, 860, 1199, 1345, 1853, 2450, 2763
BstMBI GATC 9 cut(s) 237, 780, 838, 921, 1034, 1849, 2431, 3236, 3240
BstMCI CGRYCG 2 cut(s) 240, 3239
BstMWI GCNNNNNNNGC 4 cut(s) 1211, 1289, 2237, 2268
BstNI CCWGG 5 cut(s) 229, 1222, 1632, 2754, 2798
BstNSI RCATGY 3 cut(s) 740, 2097, 2597
BstPAI GACNNNNGTC 1 cut(s) 296
BstSCI CCNGG 8 cut(s) 84, 126, 227, 752, 1220, 1630, 2752, 2796
BstSFI CTRYAG 1 cut(s) 1781
BstSLI GKGCMC 5 cut(s) 664, 748, 1228, 2815, 3266
BstV1I GCAGC 5 cut(s) 1267, 1274, 2211, 3047, 3304
BstV2I GAAGAC 5 cut(s) 546, 1437, 1886, 3019, 3215
BstX2I RGATCY 3 cut(s) 921, 1849, 2431
BstXI CCANNNNNNTGG 1 cut(s) 1750
BstYI RGATCY 3 cut(s) 921, 1849, 2431
Bsu15I ATCGAT 1 cut(s) 3239
BsuRI GGCC 6 cut(s) 5, 1601, 1629, 1672, 2345, 2721
BsuTUI ATCGAT 1 cut(s) 3239
BtgI CCRYGG 5 cut(s) 642, 1053, 1561, 2716, 3064
BtrI CACGTC 2 cut(s) 2438, 3346
BtsI GCAGTG 2 cut(s) 2226, 3010
BtsIMutI CAGTG 8 cut(s) 311, 464, 830, 1558, 2226, 2554, 3010, 3252
BveI ACCTGC 1 cut(s) 1297
Cac8I GCNNGC 5 cut(s) 423, 1541, 1674, 2242, 2312
CciI TCATGA 1 cut(s) 1141
CfoI GCGC 1 cut(s) 427
Cfr10I RCCGGY 2 cut(s) 485, 2727
Cfr13I GGNCC 3 cut(s) 1628, 2343, 2713
ClaI ATCGAT 1 cut(s) 3239
CseI GACGC 2 cut(s) 2064, 2409
Csp6I GTAC 8 cut(s) 64, 569, 1522, 2152, 2536, 2545, 2872, 3289
CviQI GTAC 8 cut(s) 64, 569, 1522, 2152, 2536, 2545, 2872, 3289
DpnI GATC 9 cut(s) 239, 782, 840, 923, 1036, 1851, 2433, 3238, 3242
DpnII GATC 9 cut(s) 237, 780, 838, 921, 1034, 1849, 2431, 3236, 3240
DraI TTTAAA 1 cut(s) 852
EaeI YGGCCR 2 cut(s) 1599, 1670
Eam1104I CTCTTC 2 cut(s) 874, 3411
EarI CTCTTC 2 cut(s) 874, 3411
Eco130I CCWWGG 4 cut(s) 642, 1053, 2716, 3064
Eco24I GRGCYC 1 cut(s) 886
Eco31I GGTCTC 4 cut(s) 494, 1199, 1345, 2763
Eco32I GATATC 2 cut(s) 1420, 2124
Eco47I GGWCC 1 cut(s) 2713
Eco57I CTGAAG 4 cut(s) 1428, 1961, 1988, 2621
Eco88I CYCGRG 1 cut(s) 7
EcoRII CCWGG 5 cut(s) 227, 1220, 1630, 2752, 2796
EcoRV GATATC 2 cut(s) 1420, 2124
EcoT14I CCWWGG 4 cut(s) 642, 1053, 2716, 3064
EcoT22I ATGCAT 2 cut(s) 1581, 2095
EcoT38I GRGCYC 1 cut(s) 886
ErhI CCWWGG 4 cut(s) 642, 1053, 2716, 3064
Esp3I CGTCTC 2 cut(s) 628, 2450
FalI AAGNNNNNCTT 6 cut(s) 405, 437, 1120, 1152, 1367, 1399
FaqI GGGAC 2 cut(s) 199, 1812
FauNDI CATATG 1 cut(s) 2999
FbaI TGATCA 1 cut(s) 780
FblI GTMKAC 3 cut(s) 602, 889, 1876
Fnu4HI GCNGC 6 cut(s) 1169, 1263, 1281, 2200, 3061, 3318
FriOI GRGCYC 1 cut(s) 886
Fsp4HI GCNGC 6 cut(s) 1169, 1263, 1281, 2200, 3061, 3318
FspBI CTAG 6 cut(s) 893, 966, 1536, 1863, 2241, 3246
FspI TGCGCA 1 cut(s) 426
GlaI GCGC 1 cut(s) 426
GluI GCNGC 6 cut(s) 1169, 1263, 1281, 2200, 3061, 3318
GsaI CCCAGC 1 cut(s) 3175
GsuI CTGGAG 2 cut(s) 1751, 2552
HaeIII GGCC 6 cut(s) 5, 1601, 1629, 1672, 2345, 2721
HapII CCGG 6 cut(s) 85, 127, 486, 606, 754, 2728
HgaI GACGC 2 cut(s) 2064, 2409
HhaI GCGC 1 cut(s) 427
Hin1I GRCGYC 1 cut(s) 188
Hin6I GCGC 1 cut(s) 425
HinP1I GCGC 1 cut(s) 425
HincII GTYRAC 2 cut(s) 2256, 2441
HindII GTYRAC 2 cut(s) 2256, 2441
HindIII AAGCTT 4 cut(s) 1530, 1911, 2066, 2260
HpaI GTTAAC 1 cut(s) 2256
HpaII CCGG 6 cut(s) 85, 127, 486, 606, 754, 2728
HphI GGTGA 7 cut(s) 101, 243, 394, 742, 1015, 1582, 2753
Hpy99I CGWCG 1 cut(s) 1272
HpyCH4III ACNGT 8 cut(s) 331, 1553, 2388, 2425, 2549, 2700, 3157, 3256
HpyCH4IV ACGT 5 cut(s) 188, 1479, 2437, 2443, 3345
HpyF10VI GCNNNNNNNGC 4 cut(s) 1211, 1289, 2237, 2268
HpySE526I ACGT 5 cut(s) 188, 1479, 2437, 2443, 3345
Hsp92I GRCGYC 1 cut(s) 188
HspAI GCGC 1 cut(s) 425
Ksp22I TGATCA 1 cut(s) 780
KspAI GTTAAC 1 cut(s) 2256
Kzo9I GATC 9 cut(s) 237, 780, 838, 921, 1034, 1849, 2431, 3236, 3240
LguI GCTCTTC 1 cut(s) 874
LmnI GCTCC 4 cut(s) 55, 202, 384, 1310
Lsp1109I GCAGC 5 cut(s) 1267, 1274, 2211, 3047, 3304
LweI GCATC 5 cut(s) 32, 1090, 1566, 2080, 2872
MaeI CTAG 6 cut(s) 893, 966, 1536, 1863, 2241, 3246
MaeII ACGT 5 cut(s) 188, 1479, 2437, 2443, 3345
MaeIII GTNAC 7 cut(s) 1151, 2083, 2107, 2162, 2458, 2509, 2745
MalI GATC 9 cut(s) 239, 782, 840, 923, 1036, 1851, 2433, 3238, 3242
MboI GATC 9 cut(s) 237, 780, 838, 921, 1034, 1849, 2431, 3236, 3240
MfeI CAATTG 5 cut(s) 1449, 2338, 3113, 3292, 3459
MflI RGATCY 3 cut(s) 921, 1849, 2431
MhlI GDGCHC 8 cut(s) 14, 664, 748, 886, 1228, 2815, 3143, 3266
MlsI TGGCCA 2 cut(s) 1601, 1672
MluNI TGGCCA 2 cut(s) 1601, 1672
MlyI GAGTC 2 cut(s) 211, 2455
MmeI TCCRAC 1 cut(s) 2058
Mox20I TGGCCA 2 cut(s) 1601, 1672
Mph1103I ATGCAT 2 cut(s) 1581, 2095
MroXI GAANNNNTTC 3 cut(s) 1071, 2496, 3420
MscI TGGCCA 2 cut(s) 1601, 1672
MslI CAYNNNNRTG 4 cut(s) 741, 2567, 3257, 3282
Msp20I TGGCCA 2 cut(s) 1601, 1672
MspCI CTTAAG 2 cut(s) 2069, 2771
MspI CCGG 6 cut(s) 85, 127, 486, 606, 754, 2728
MspR9I CCNGG 8 cut(s) 86, 128, 229, 754, 1222, 1632, 2754, 2798
MunI CAATTG 5 cut(s) 1449, 2338, 3113, 3292, 3459
Mva1269I GAATGC 3 cut(s) 1934, 2242, 2312
MvaI CCWGG 5 cut(s) 229, 1222, 1632, 2754, 2798
MwoI GCNNNNNNNGC 4 cut(s) 1211, 1289, 2237, 2268
NciI CCSGG 3 cut(s) 86, 128, 754
NcoI CCATGG 4 cut(s) 642, 1053, 2716, 3064
NdeI CATATG 1 cut(s) 2999
NdeII GATC 9 cut(s) 237, 780, 838, 921, 1034, 1849, 2431, 3236, 3240
NheI GCTAGC 1 cut(s) 2240
NlaIV GGNNCC 5 cut(s) 357, 1109, 2433, 2714, 3423
NmuCI GTSAC 3 cut(s) 2162, 2458, 2509
NsbI TGCGCA 1 cut(s) 426
NsiI ATGCAT 2 cut(s) 1581, 2095
NspI RCATGY 3 cut(s) 740, 2097, 2597
OliI CACNNNNGTG 1 cut(s) 3257
PaeR7I CTCGAG 1 cut(s) 7
PagI TCATGA 1 cut(s) 1141
PasI CCCWGGG 1 cut(s) 1631
PciI ACATGT 2 cut(s) 736, 2593
PciSI GCTCTTC 1 cut(s) 874
PcsI WCGNNNNNNNCGW 1 cut(s) 2882
PctI GAATGC 3 cut(s) 1934, 2242, 2312
PdmI GAANNNNTTC 3 cut(s) 1071, 2496, 3420
PfeI GAWTC 8 cut(s) 224, 503, 1145, 1804, 1981, 2328, 2903, 3164
PflMI CCANNNNNTGG 3 cut(s) 1054, 1513, 1567
PkrI GCNGC 6 cut(s) 1170, 1264, 1282, 2201, 3062, 3319
Ple19I CGATCG 2 cut(s) 240, 3239
PleI GAGTC 2 cut(s) 210, 2455
PpsI GAGTC 2 cut(s) 210, 2455
Ppu21I YACGTR 1 cut(s) 1480
PscI ACATGT 2 cut(s) 736, 2593
PshAI GACNNNNGTC 1 cut(s) 296
PshBI ATTAAT 2 cut(s) 1680, 2010
PsiI TTATAA 1 cut(s) 929
Psp6I CCWGG 5 cut(s) 227, 1220, 1630, 2752, 2796
PspFI CCCAGC 1 cut(s) 3171
PspGI CCWGG 5 cut(s) 227, 1220, 1630, 2752, 2796
PspN4I GGNNCC 5 cut(s) 357, 1109, 2433, 2714, 3423
PspPI GGNCC 3 cut(s) 1628, 2343, 2713
PspXI VCTCGAGB 1 cut(s) 7
PsuI RGATCY 3 cut(s) 921, 1849, 2431
PvuI CGATCG 2 cut(s) 240, 3239
RsaI GTAC 8 cut(s) 65, 570, 1523, 2153, 2537, 2546, 2873, 3290
RsaNI GTAC 8 cut(s) 64, 569, 1522, 2152, 2536, 2545, 2872, 3289
RseI CAYNNNNRTG 4 cut(s) 741, 2567, 3257, 3282
SapI GCTCTTC 1 cut(s) 874
SatI GCNGC 6 cut(s) 1169, 1263, 1281, 2200, 3061, 3318
Sau3AI GATC 9 cut(s) 237, 780, 838, 921, 1034, 1849, 2431, 3236, 3240
Sau96I GGNCC 3 cut(s) 1628, 2343, 2713
SchI GAGTC 2 cut(s) 211, 2455
ScrFI CCNGG 8 cut(s) 86, 128, 229, 754, 1222, 1632, 2754, 2798
SduI GDGCHC 8 cut(s) 14, 664, 748, 886, 1228, 2815, 3143, 3266
SfaNI GCATC 5 cut(s) 32, 1090, 1566, 2080, 2872
SfcI CTRYAG 1 cut(s) 1781
Sfr274I CTCGAG 1 cut(s) 7
SinI GGWCC 1 cut(s) 2713
SlaI CTCGAG 1 cut(s) 7
SmiMI CAYNNNNRTG 4 cut(s) 741, 2567, 3257, 3282
SmlI CTYRAG 5 cut(s) 7, 1348, 2069, 2501, 2771
SmoI CTYRAG 5 cut(s) 7, 1348, 2069, 2501, 2771
SpeI ACTAGT 2 cut(s) 892, 1862
SsiI CCGC 2 cut(s) 1168, 3400
SspI AATATT 3 cut(s) 820, 1464, 1684
SspMI CTAG 6 cut(s) 893, 966, 1536, 1863, 2241, 3246
StyD4I CCNGG 8 cut(s) 84, 126, 227, 752, 1220, 1630, 2752, 2796
StyI CCWWGG 4 cut(s) 642, 1053, 2716, 3064
TaaI ACNGT 8 cut(s) 331, 1553, 2388, 2425, 2549, 2700, 3157, 3256
TaiI ACGT 5 cut(s) 191, 1482, 2440, 2446, 3348
TaqI TCGA 8 cut(s) 8, 537, 1037, 1148, 1267, 1591, 3162, 3239
TatI WGTACW 4 cut(s) 2151, 2535, 2871, 3288
TauI GCSGC 1 cut(s) 1171
TfiI GAWTC 8 cut(s) 224, 503, 1145, 1804, 1981, 2328, 2903, 3164
TscAI CASTG 8 cut(s) 311, 464, 837, 1558, 2233, 2554, 3010, 3259
TseFI GTSAC 3 cut(s) 2162, 2458, 2509
TseI GCWGC 5 cut(s) 1262, 1280, 2199, 3060, 3317
Tsp45I GTSAC 3 cut(s) 2162, 2458, 2509
TspGWI ACGGA 5 cut(s) 1578, 2102, 2412, 2891, 3020
TspRI CASTG 8 cut(s) 311, 464, 837, 1558, 2233, 2554, 3010, 3259
Van91I CCANNNNNTGG 3 cut(s) 1054, 1513, 1567
Vha464I CTTAAG 2 cut(s) 2069, 2771
VneI GTGCAC 2 cut(s) 744, 2811
VpaK11BI GGWCC 1 cut(s) 2713
VspI ATTAAT 2 cut(s) 1680, 2010
XapI RAATTY 3 cut(s) 1728, 2375, 2827
XbaI TCTAGA 1 cut(s) 3245
XceI RCATGY 3 cut(s) 740, 2097, 2597
XcmI CCANNNNNNNNNTGG 1 cut(s) 1507
XhoI CTCGAG 1 cut(s) 7
XmiI GTMKAC 3 cut(s) 602, 889, 1876
XmnI GAANNNNTTC 3 cut(s) 1071, 2496, 3420
XspI CTAG 6 cut(s) 893, 966, 1536, 1863, 2241, 3246
ZraI GACGTC 1 cut(s) 189
Zsp2I ATGCAT 2 cut(s) 1581, 2095
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.