FvH4_7g06680

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000085
Physical Location & Seq
Forward (+)
6726996 .. 6731009
4014 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g06680.t1

Sequence Viewer

Length: 3483 bp
ATGCAATTCTATTCCTTCCAGATGGCCATGGCGATTATTGTGGTAATTGTGACATGGATCGGTGTCATAGCTACTAGGAAGTGGATATGGACTTTAGTGAAGCATCGGTTCAGTTACATGGTTGAAAAACGTGAGCCAATATCTCAGGATAATCAAGATTTAGTCGACAGATCTCTTCCTTCATCATCAGCTCGCCAATGGAATTATGATGTGTTCTTGAGTTTCAGGGGTCCAGACACTCGCAAGGGTATTACATTTGAGTTATACGATCGACTGCACAGGAGGGGAATCAAAACATTCATGGATGACCAAGATCTTCAAGTTGGGGATACTATTTCTCCCACTCTACTGAAAGCAATTGAAGCATCAAAGTTGGCAATTGTTGTTCTGTCTGAAAAGTATGCTTCTTCTACTTGGTGCTTGGAGGAGCTTACAAAGATCTGTGAGTGCATGAAAGATGACAACAGGATTCTGCCACTTTTCTATAATGTTGAGCCTACTGATGTAAGATATCAGAAGAGAAGTTTTGCAGAAGCATTCACCAGATATGAATTCTCTGGGCGACAAAGACCAGAGAAGGTCAAGCAGTGGAGAGATGCTTTAAACAAAGTGGCTCATTTCTCTGGTTGGGATTCAAAGAAATACAAGACTGAAAGAGAACTCGTCGAAGACATTGTGGAATATGTGTTTAGTAAAGTGCAACCTGTTGCAATCAACATGGGAGATTTTGAAGAATTTGAAGCAACAAGACAAGCCATGAATATGGTTATGAGAGGGCTAAAAGATGACAAGGTCACTGCCATTGGAGTCTACGGAATGGGAGGTGTCGGCAAGACAACCTTGGTAAAACATGTGAGTGATCAAGCTCGGAAAAATGGGATCTTTCATCATGTGATTATGGCTACTATATCACAAATCCCGGACTTGAGAAAAATTCAAGGCCAATTGGCAGATCTGTTGGGCTTTGAATTCATGGAGGAGACTGAAATTGGAAGAGCAGCTAGATTGACTAAGGAGATAATGAGAAGAAATAAGATTCTTGTAATCCTTGATGACATTTGGGAGAGAATGGATATGTCAATAATAGGAATACCTAGCTACGAGGAACTTCAAAAGTGCAATTCTAAAGTCCTACTCACCACAAGGATATGGAATGTCTGTCATGTCATGAGATGCCAAGAAAAGATCACCCTCAATATCCTATCAAAAGATGATTCTTGGACATTATTTGTGAGAAATGCAGGAAGGTCTTTTTTATCCACCAATTTTGAGAATGTAGCAAGGAAGGTAGCGGGAGAATGTAGGGGTCTACCCATAGCATTGATAGCGGTTGCAAAGGCGCTCGGAGATAAAGACTTGGTGGAATGGGAAAGAGCAGCTCAACGACTAGAGAAGTCACAAACTGCCAACCCTGACGATAAAGGTGATGCATCTGAATGTATAAGATTAAGTTATGATTACTTGAAAAATGAGGATTATAGATCATGCTTCTTGCTATGTTGCTTATTCCCAGAAGACTATGACATCCAAGTAGAAACCTTGTTCAGCTATGTGATAGGAAAAGGATTGTTTAGAGATGCTGAGACCATAGACGAAGCCAGAGGAACAACAAATTTGGTGGTCGCCCACCTAAAAAATTGTAGCTTGCTTTTGGATAGTGAAGAAGATGGATGTGTAAGGATGCATGATGTCATTCGTGATACTGCCATGATGATTGCAAAATCTAAAGATGGGCACGGGTTTTTGGTAAAAGCTGGGTCTGGTTTAAAGCATTGGTCATGTCGATTACATGAAGGCTACTCTGCAATCTCACTAATGAAGAACAAACTTCGCAAGCTACCTGAAGAGTTGGTATGTCCAAAACTCCAGATTTTATTACTACAAGAGAATGCTGATTTAGATGAGATTCCAGAAAAGTTTATCCAAAGTGCAAATGAGTTAAAGGTCTTAGATCTTGGCCTCACTAGTATTTTATCACTACCGCAATCATTGAGTCTGCTTACAAACCTCCAAGCTTTGTATTTAGATTTTTGCAAGAAAAGAGTTGACATTTCCATAATTGGAAAACTCACTAACCTTGAGATTCTTACTATGAGAGGTTATCCTGTTAAGGATTTGTCAAGAGAATTGTGGAGAGAAGTAGGAAAATTGACAAATCTAAGGATGTTGGATTTCGGTGGCATTAGTTTGGTGGGTGGAGGTACTGTCACAATTCCCTCTAAGGTGATATCAAAGTTGCAGAGATTAGAAGAACTGTACATGCTGTACTGTGGATTTGAGGTCTGGGGAAGTCACGTTGAGGGAGAAGTAGAAGAAACATATATCGGCTTTGATGAGTTAACTAGATTATCAAATTTGAAAATTGTGCAGGTTTGCATATCTGATGCAAAGTGCATCCCAAAAGAAGTTGAGGTTGAACCAAATTGGGTTTACTTTGATATACGTATCGATGGCAGCACAAGCAATCCTTACAAGCGAAATGATCTCAACTCAAGATCCTCATCTCTTAACACAACCATCAGTAGCTTACCAGACTGGTTTGTCAATGTGGTGACAGAGAAATCAGAGAAGCTATTGTATGAAAATAACTGCAAAGGGTTAAGTAACATTCTTATGGAATATGACCATGGGAGGTTACATGGATTGAAGCATCTCTCTGTAATTGGTCCCAATAAAAACTTGAAAGAGTTGATGAACACAACAACACGGATTAATCAAGAACCTGTGTTTGAGAGCTTGGAAGAGTTGCATCTGAGTTGTTTGGACTGCATGAAGGAGCTGTGTGTTGGTGACTTACCAGCTGGTTCTCTATTCAACATGAAGTTATTGAGGATCCGTGATTGTCGTGACTTGGGGAATGTACTGTTGCCTTCAAAATTGTTGCAGAGACTACCAAATCTGGAAGTAGTATTTCTTTATAGAATATGCAAATTGGAATATGTGTTTGGATGCAGCGAATTTGAGCCAGAACATTCAAAATTGAGAGAGGTGACATTGTTGGAGCTAGATGCAGTAAGAAGCATATGTAATGGTCAGGTTCCCCATGTTATGTTCTGGACTCTTAAAAGTTTGTTCATATACCATTGCAAGTCTTTGGGAAGTCTATTCACATATGATGCAGCGGAATGTCTTTTTCAATTGGAAGAGCTTTGGATAGAGACATGCCCCTTGTTGGATAGAGTAATTGAAACAAGCAAGGCAACAGTTAACAACAACAACAACAAAAACAAGAAAATTGTTCTTTCAAAATTAAAGAAGTTAGTTTTGAAGGAACTTCCTGTGCTGTACAGTGGAGGTGCTACTACTGAAATTGATATCGAGTGTCCTTCACTGGAGCACTTGTATGTGAAGGACTGCCCCCATTTATCATTTCCAACCTCTGCTTCTGACTATTTCCACAGCAGGAACCAAATCCAACTCAATGATCAACAACAGTACGACGTTCTATATTACAGAATTTGGAGTCCAACATTGGAAAGCTGA

Protein Analysis

1161

Amino Acids

132.97

Weight (kDa)

6.12

Isoelectric Point (pI)

38.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 68 - 238 1.7e-47 TIR domain
TIR_2 PF13676 71 - 167 1.4e-15 TIR domain
NB-ARC PF00931 252 - 415 6.4e-28 NB-ARC domain
LRR_8 PF13855 620 - 678 2.7e-06 Leucine rich repeat
LRR_14 PF23598 625 - 792 2.2e-12 Leucine-rich repeat region
LRR_RPS2 PF23247 913 - 1055 1.1e-17 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2540
Acc36I ACCTGC 1 cut(s) 2359
AccI GTMKAC 3 cut(s) 165, 810, 1309
AciI CCGC 4 cut(s) 1292, 1328, 1982, 3122
AclWI GGATC 5 cut(s) 65, 887, 2490, 2827, 2840
AcoI YGGCCR 1 cut(s) 24
AcsI RAATTY 8 cut(s) 551, 734, 933, 968, 1612, 2353, 2957, 3456
AcuI CTGAAG 1 cut(s) 1863
AfaI GTAC 6 cut(s) 2203, 2258, 2267, 2862, 3287, 3437
AfiI CCNNNNNNNGG 1 cut(s) 3172
AflIII ACRYGT 1 cut(s) 850
AhlI ACTAGT 1 cut(s) 1964
AjuI GAANNNNNNNTTGG 4 cut(s) 2928, 2960, 3367, 3399
Alw21I GWGCWC 1 cut(s) 3339
Alw26I GTCTC 4 cut(s) 974, 1577, 2881, 3152
AlwI GGATC 5 cut(s) 65, 887, 2490, 2827, 2840
AoxI GGCC 3 cut(s) 24, 940, 1957
ApeKI GCWGC 5 cut(s) 998, 1376, 2454, 2952, 3119
ApoI RAATTY 8 cut(s) 551, 734, 933, 968, 1612, 2353, 2957, 3456
AseI ATTAAT 1 cut(s) 2712
AspLEI GCGC 1 cut(s) 1342
AspS9I GGNCC 2 cut(s) 230, 2666
AsuC2I CCSGG 1 cut(s) 920
AsuHPI GGTGA 8 cut(s) 532, 1129, 1180, 1436, 2236, 2563, 2801, 3001
AvaII GGWCC 2 cut(s) 230, 2666
BaeGI GKGCMC 1 cut(s) 1737
BalI TGGCCA 1 cut(s) 26
BamHI GGATCC 1 cut(s) 2832
BbsI GAAGAC 2 cut(s) 675, 1521
Bbv12I GWGCWC 1 cut(s) 3339
BbvI GCAGC 5 cut(s) 1010, 1388, 2466, 2964, 3131
BccI CCATC 5 cut(s) 16, 1661, 1724, 2444, 2525
BciVI GTATCC 1 cut(s) 322
BclI TGATCA 2 cut(s) 859, 3424
BcnI CCSGG 1 cut(s) 920
BcoDI GTCTC 4 cut(s) 974, 1577, 2881, 3152
BcuI ACTAGT 1 cut(s) 1964
BfaI CTAG 7 cut(s) 75, 1002, 1095, 1388, 1965, 2343, 3005
BfoI RGCGCY 1 cut(s) 1343
BfuAI ACCTGC 1 cut(s) 2359
BfuI GTATCC 1 cut(s) 322
BglII AGATCT 5 cut(s) 170, 313, 438, 952, 1951
BisI GCNGC 5 cut(s) 999, 1377, 2455, 2953, 3120
BlsI GCNGC 5 cut(s) 1000, 1378, 2456, 2954, 3121
Bme1390I CCNGG 1 cut(s) 920
Bme18I GGWCC 2 cut(s) 230, 2666
BmgT120I GGNCC 2 cut(s) 230, 2666
BmiI GGNNCC 5 cut(s) 231, 2668, 2834, 3039, 3407
BmrFI CCNGG 1 cut(s) 920
BpiI GAAGAC 2 cut(s) 675, 1521
BpmI CTGGAG 2 cut(s) 1850, 3353
BpuEI CTTGAG 4 cut(s) 238, 946, 2099, 2476
BpuMI CCSGG 1 cut(s) 920
Bsa29I ATCGAT 1 cut(s) 2448
BsaAI YACGTR 1 cut(s) 2444
BsaBI GATNNNNATC 1 cut(s) 2500
BsaI GGTCTC 1 cut(s) 1577
BsaJI CCNNGG 3 cut(s) 27, 840, 2626
BsaXI ACNNNNNCTCC 6 cut(s) 322, 352, 2190, 2220, 3326, 3356
Bsc4I CCNNNNNNNGG 1 cut(s) 3172
Bse1I ACTGG 2 cut(s) 2540, 3336
Bse3DI GCAATG 1 cut(s) 3082
Bse8I GATNNNNATC 1 cut(s) 2500
BseCI ATCGAT 1 cut(s) 2448
BseDI CCNNGG 3 cut(s) 27, 840, 2626
BseGI GGATG 7 cut(s) 310, 1524, 1676, 1686, 2169, 2394, 2954
BseJI GATNNNNATC 1 cut(s) 2500
BseLI CCNNNNNNNGG 1 cut(s) 3172
BseMI GCAATG 1 cut(s) 3082
BseMII CTCAG 3 cut(s) 158, 1572, 2744
BseNI ACTGG 2 cut(s) 2540, 3336
BseRI GAGGAG 2 cut(s) 440, 992
BseSI GKGCMC 1 cut(s) 1737
BseXI GCAGC 5 cut(s) 1010, 1388, 2466, 2964, 3131
BseYI CCCAGC 1 cut(s) 1754
BsgI GTGCAG 2 cut(s) 260, 2387
Bsh1285I CGRYCG 1 cut(s) 271
BshFI GGCC 3 cut(s) 26, 942, 1959
BshVI ATCGAT 1 cut(s) 2448
BsiEI CGRYCG 1 cut(s) 271
BsiHKAI GWGCWC 1 cut(s) 3339
BsiSI CCGG 1 cut(s) 920
BslFI GGGAC 1 cut(s) 2652
BslI CCNNNNNNNGG 1 cut(s) 3172
BsmAI GTCTC 4 cut(s) 974, 1577, 2881, 3152
BsmFI GGGAC 1 cut(s) 2652
BsmI GAATGC 2 cut(s) 536, 1894
BsnI GGCC 3 cut(s) 26, 942, 1959
Bso31I GGTCTC 1 cut(s) 1577
Bsp1286I GDGCHC 2 cut(s) 1737, 3339
Bsp1407I TGTACA 2 cut(s) 2256, 3285
Bsp19I CCATGG 2 cut(s) 27, 2626
BspACI CCGC 4 cut(s) 1292, 1328, 1982, 3122
BspANI GGCC 3 cut(s) 26, 942, 1959
BspCNI CTCAG 3 cut(s) 157, 1573, 2745
BspDI ATCGAT 1 cut(s) 2448
BspHI TCATGA 1 cut(s) 1167
BspLI GGNNCC 5 cut(s) 231, 2668, 2834, 3039, 3407
BspMI ACCTGC 1 cut(s) 2359
BspPI GGATC 5 cut(s) 65, 887, 2490, 2827, 2840
BspQI GCTCTTC 2 cut(s) 988, 3138
BspTNI GGTCTC 1 cut(s) 1577
BsrDI GCAATG 1 cut(s) 3082
BsrGI TGTACA 2 cut(s) 2256, 3285
BsrI ACTGG 2 cut(s) 2540, 3336
BssECI CCNNGG 3 cut(s) 27, 840, 2626
BssT1I CCWWGG 3 cut(s) 27, 840, 2626
Bst4CI ACNGT 7 cut(s) 2206, 2256, 2270, 2865, 3205, 3290, 3435
Bst6I CTCTTC 6 cut(s) 180, 512, 988, 1839, 2736, 3138
BstAUI TGTACA 2 cut(s) 2256, 3285
BstBAI YACGTR 1 cut(s) 2444
BstC8I GCNNGC 3 cut(s) 193, 1646, 1835
BstDEI CTNAG 7 cut(s) 144, 1011, 1581, 1948, 2159, 2220, 2753
BstDSI CCRYGG 2 cut(s) 27, 2626
BstF5I GGATG 7 cut(s) 310, 1524, 1676, 1686, 2169, 2394, 2954
BstH2I RGCGCY 1 cut(s) 1343
BstHHI GCGC 1 cut(s) 1342
BstMAI GTCTC 4 cut(s) 974, 1577, 2881, 3152
BstMCI CGRYCG 1 cut(s) 271
BstMWI GCNNNNNNNGC 3 cut(s) 362, 1325, 2460
BstNSI RCATGY 3 cut(s) 854, 2263, 3165
BstSCI CCNGG 1 cut(s) 918
BstSLI GKGCMC 1 cut(s) 1737
BstSNI TACGTA 1 cut(s) 2444
BstV1I GCAGC 5 cut(s) 1010, 1388, 2466, 2964, 3131
BstV2I GAAGAC 2 cut(s) 675, 1521
BstX2I RGATCY 8 cut(s) 170, 313, 438, 879, 952, 1951, 2495, 2832
BstXI CCANNNNNNTGG 1 cut(s) 763
BstYI RGATCY 8 cut(s) 170, 313, 438, 879, 952, 1951, 2495, 2832
Bsu15I ATCGAT 1 cut(s) 2448
BsuI GTATCC 1 cut(s) 322
BsuRI GGCC 3 cut(s) 26, 942, 1959
BsuTUI ATCGAT 1 cut(s) 2448
BtgI CCRYGG 2 cut(s) 27, 2626
BtsCI GGATG 7 cut(s) 310, 1524, 1676, 1686, 2169, 2394, 2954
BtsI GCAGTG 2 cut(s) 593, 795
BtsIMutI CAGTG 4 cut(s) 593, 795, 3295, 3329
BveI ACCTGC 1 cut(s) 2359
Cac8I GCNNGC 3 cut(s) 193, 1646, 1835
CciI TCATGA 1 cut(s) 1167
CfoI GCGC 1 cut(s) 1342
Cfr13I GGNCC 2 cut(s) 230, 2666
ClaI ATCGAT 1 cut(s) 2448
Csp6I GTAC 6 cut(s) 2202, 2257, 2266, 2861, 3286, 3436
CspCI CAANNNNNGTGG 4 cut(s) 1249, 1284, 1599, 1634
CviQI GTAC 6 cut(s) 2202, 2257, 2266, 2861, 3286, 3436
DdeI CTNAG 7 cut(s) 144, 1011, 1581, 1948, 2159, 2220, 2753
DraI TTTAAA 2 cut(s) 603, 1767
DrdI GACNNNNNNGTC 1 cut(s) 2540
DseDI GACNNNNNNGTC 1 cut(s) 2540
EaeI YGGCCR 1 cut(s) 24
Eam1104I CTCTTC 6 cut(s) 180, 512, 988, 1839, 2736, 3138
EarI CTCTTC 6 cut(s) 180, 512, 988, 1839, 2736, 3138
Eco105I TACGTA 1 cut(s) 2444
Eco130I CCWWGG 3 cut(s) 27, 840, 2626
Eco31I GGTCTC 1 cut(s) 1577
Eco32I GATATC 3 cut(s) 512, 2229, 3316
Eco47I GGWCC 2 cut(s) 230, 2666
Eco57I CTGAAG 1 cut(s) 1863
EcoRI GAATTC 2 cut(s) 551, 968
EcoRV GATATC 3 cut(s) 512, 2229, 3316
EcoT14I CCWWGG 3 cut(s) 27, 840, 2626
EcoT22I ATGCAT 2 cut(s) 1432, 1686
ErhI CCWWGG 3 cut(s) 27, 840, 2626
FalI AAGNNNNNCTT 4 cut(s) 824, 856, 2452, 2484
FaqI GGGAC 1 cut(s) 2652
FauI CCCGC 1 cut(s) 1285
FauNDI CATATG 2 cut(s) 3023, 3112
FbaI TGATCA 2 cut(s) 859, 3424
FblI GTMKAC 3 cut(s) 165, 810, 1309
Fnu4HI GCNGC 5 cut(s) 999, 1377, 2455, 2953, 3120
FokI GGATG 7 cut(s) 317, 1511, 1683, 1693, 2176, 2381, 2961
Fsp4HI GCNGC 5 cut(s) 999, 1377, 2455, 2953, 3120
FspBI CTAG 7 cut(s) 75, 1002, 1095, 1388, 1965, 2343, 3005
GlaI GCGC 1 cut(s) 1341
GluI GCNGC 5 cut(s) 999, 1377, 2455, 2953, 3120
GsaI CCCAGC 1 cut(s) 1758
GsuI CTGGAG 2 cut(s) 1850, 3353
HaeII RGCGCY 1 cut(s) 1343
HaeIII GGCC 3 cut(s) 26, 942, 1959
HapII CCGG 1 cut(s) 920
HhaI GCGC 1 cut(s) 1342
Hin6I GCGC 1 cut(s) 1340
HinP1I GCGC 1 cut(s) 1340
HincII GTYRAC 4 cut(s) 166, 2047, 2340, 3208
HindII GTYRAC 4 cut(s) 166, 2047, 2340, 3208
HindIII AAGCTT 1 cut(s) 2013
HpaI GTTAAC 2 cut(s) 2340, 3208
HpaII CCGG 1 cut(s) 920
HphI GGTGA 8 cut(s) 532, 1129, 1180, 1436, 2236, 2563, 2801, 3001
Hpy166II GTNNAC 7 cut(s) 166, 811, 1310, 2047, 2340, 2431, 3208
Hpy188I TCNGA 9 cut(s) 394, 516, 870, 1346, 1435, 2383, 2566, 2754, 3388
Hpy8I GTNNAC 7 cut(s) 166, 811, 1310, 2047, 2340, 2431, 3208
Hpy99I CGWCG 2 cut(s) 668, 3443
HpyCH4III ACNGT 7 cut(s) 2206, 2256, 2270, 2865, 3205, 3290, 3435
HpyCH4IV ACGT 4 cut(s) 130, 2295, 2443, 3441
HpyF10VI GCNNNNNNNGC 3 cut(s) 362, 1325, 2460
HpyF3I CTNAG 7 cut(s) 144, 1011, 1581, 1948, 2159, 2220, 2753
HpySE526I ACGT 4 cut(s) 130, 2295, 2443, 3441
HspAI GCGC 1 cut(s) 1340
Ksp22I TGATCA 2 cut(s) 859, 3424
KspAI GTTAAC 2 cut(s) 2340, 3208
LguI GCTCTTC 2 cut(s) 988, 3138
LmnI GCTCC 4 cut(s) 427, 2776, 3001, 3334
Lsp1109I GCAGC 5 cut(s) 1010, 1388, 2466, 2964, 3131
MaeI CTAG 7 cut(s) 75, 1002, 1095, 1388, 1965, 2343, 3005
MaeII ACGT 4 cut(s) 130, 2295, 2443, 3441
MfeI CAATTG 4 cut(s) 357, 378, 944, 3137
MflI RGATCY 8 cut(s) 170, 313, 438, 879, 952, 1951, 2495, 2832
MhlI GDGCHC 2 cut(s) 1737, 3339
MlsI TGGCCA 1 cut(s) 26
MluNI TGGCCA 1 cut(s) 26
MlyI GAGTC 4 cut(s) 816, 2002, 3052, 3472
MmeI TCCRAC 5 cut(s) 2148, 2979, 3153, 3398, 3439
Mox20I TGGCCA 1 cut(s) 26
Mph1103I ATGCAT 2 cut(s) 1432, 1686
MscI TGGCCA 1 cut(s) 26
MslI CAYNNNNRTG 5 cut(s) 761, 855, 1435, 2612, 3342
Msp20I TGGCCA 1 cut(s) 26
MspA1I CMGCKG 2 cut(s) 2801, 3122
MspI CCGG 1 cut(s) 920
MspR9I CCNGG 1 cut(s) 920
MunI CAATTG 4 cut(s) 357, 378, 944, 3137
Mva1269I GAATGC 2 cut(s) 536, 1894
MwoI GCNNNNNNNGC 3 cut(s) 362, 1325, 2460
NciI CCSGG 1 cut(s) 920
NcoI CCATGG 2 cut(s) 27, 2626
NdeI CATATG 2 cut(s) 3023, 3112
NlaIV GGNNCC 5 cut(s) 231, 2668, 2834, 3039, 3407
NmuCI GTSAC 9 cut(s) 49, 793, 1395, 2206, 2291, 2551, 2789, 2846, 2989
NsiI ATGCAT 2 cut(s) 1432, 1686
NspI RCATGY 3 cut(s) 854, 2263, 3165
PagI TCATGA 1 cut(s) 1167
PciI ACATGT 1 cut(s) 850
PciSI GCTCTTC 2 cut(s) 988, 3138
PctI GAATGC 2 cut(s) 536, 1894
PfeI GAWTC 7 cut(s) 288, 469, 632, 1036, 1214, 1906, 2083
PflFI GACNNNGTC 1 cut(s) 791
PfoI TCCNGGA 1 cut(s) 918
PkrI GCNGC 5 cut(s) 1000, 1378, 2456, 2954, 3121
Ple19I CGATCG 1 cut(s) 271
PleI GAGTC 4 cut(s) 815, 2001, 3052, 3471
PpsI GAGTC 4 cut(s) 815, 2001, 3052, 3471
Ppu21I YACGTR 1 cut(s) 2444
PscI ACATGT 1 cut(s) 850
PshBI ATTAAT 1 cut(s) 2712
PspFI CCCAGC 1 cut(s) 1754
PspN4I GGNNCC 5 cut(s) 231, 2668, 2834, 3039, 3407
PspPI GGNCC 2 cut(s) 230, 2666
PsuI RGATCY 8 cut(s) 170, 313, 438, 879, 952, 1951, 2495, 2832
PsyI GACNNNGTC 1 cut(s) 791
PvuI CGATCG 1 cut(s) 271
PvuII CAGCTG 1 cut(s) 2801
RsaI GTAC 6 cut(s) 2203, 2258, 2267, 2862, 3287, 3437
RsaNI GTAC 6 cut(s) 2202, 2257, 2266, 2861, 3286, 3436
RseI CAYNNNNRTG 5 cut(s) 761, 855, 1435, 2612, 3342
SalI GTCGAC 1 cut(s) 164
SapI GCTCTTC 2 cut(s) 988, 3138
SatI GCNGC 5 cut(s) 999, 1377, 2455, 2953, 3120
Sau96I GGNCC 2 cut(s) 230, 2666
SchI GAGTC 4 cut(s) 816, 2002, 3052, 3472
ScrFI CCNGG 1 cut(s) 920
SduI GDGCHC 2 cut(s) 1737, 3339
SinI GGWCC 2 cut(s) 230, 2666
SmiMI CAYNNNNRTG 5 cut(s) 761, 855, 1435, 2612, 3342
SmlI CTYRAG 4 cut(s) 217, 925, 2078, 2491
SmoI CTYRAG 4 cut(s) 217, 925, 2078, 2491
SnaBI TACGTA 1 cut(s) 2444
SpeI ACTAGT 1 cut(s) 1964
SsiI CCGC 4 cut(s) 1292, 1328, 1982, 3122
SspMI CTAG 7 cut(s) 75, 1002, 1095, 1388, 1965, 2343, 3005
StyD4I CCNGG 1 cut(s) 918
StyI CCWWGG 3 cut(s) 27, 840, 2626
TaaI ACNGT 7 cut(s) 2206, 2256, 2270, 2865, 3205, 3290, 3435
TaiI ACGT 4 cut(s) 133, 2298, 2446, 3444
TaqI TCGA 6 cut(s) 165, 271, 666, 1783, 2448, 3318
TatI WGTACW 4 cut(s) 2256, 2265, 2860, 3285
TfiI GAWTC 7 cut(s) 288, 469, 632, 1036, 1214, 1906, 2083
TscAI CASTG 4 cut(s) 593, 802, 3295, 3336
TseFI GTSAC 9 cut(s) 49, 793, 1395, 2206, 2291, 2551, 2789, 2846, 2989
TseI GCWGC 5 cut(s) 998, 1376, 2454, 2952, 3119
Tsp45I GTSAC 9 cut(s) 49, 793, 1395, 2206, 2291, 2551, 2789, 2846, 2989
TspGWI ACGGA 3 cut(s) 828, 2722, 2825
TspRI CASTG 4 cut(s) 593, 802, 3295, 3336
Tth111I GACNNNGTC 1 cut(s) 791
VpaK11BI GGWCC 2 cut(s) 230, 2666
VspI ATTAAT 1 cut(s) 2712
XapI RAATTY 8 cut(s) 551, 734, 933, 968, 1612, 2353, 2957, 3456
XceI RCATGY 3 cut(s) 854, 2263, 3165
XmiI GTMKAC 3 cut(s) 165, 810, 1309
XspI CTAG 7 cut(s) 75, 1002, 1095, 1388, 1965, 2343, 3005
Zsp2I ATGCAT 2 cut(s) 1432, 1686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.