Rmu_sc0005194.1_g000002

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005194.1
OG0000085
Physical Location & Seq
Forward (+)
1788 .. 6760
4973 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005194.1_g000002.1.cds

Sequence Viewer

Length: 3432 bp
atgagcttttgcaagagatttgttcatggttatgatgattttaggggccggaatctggaatcggtgatttttggggagggaggcaggtttgggtcgagcaacgcgaagaagagagaaatgaggctgaggttgagagttgagacgtcgatggtcgggagccgagtctttgtggaggaggatgtgggttcgaaccctgcctcttacactattttgtacatatactatgtttttcagtatgtatacattatattcggtcggtacggtataccgacggtatgccatacgccataccgtagccgagccgaaggaatacatacggtacggttgggccgtaccgaaagtcggcaggccgagatttcggcccaaatcggttcgggccggttcgaatttcggggtgtagacactcgcaaggggattacagccgacatacacgatcgacttaataggagtggaatcaaaacattcatggatgagcaagaccttctagtaggggatgttatttcccctactctcttagcagcaatcaaagaatcaaggtttgcaattgttgttctctcgcaaaattatgcttcttctacttggtgtttggaggaacttagagagatctgtgagtgcatgaaagaccagaacagaattttgccacttttctatcacgtggagccctctgatgttcgatatcagaagaagaggagtttcgaagaagctttctctaagcatgaaacctctggtcgacatgaattagagaaggtgaagcagtggagggatgctttgtacaaagtggcaaatttctctgggtggaatacatctgattataagactcacaaggaacttgttgacaacattgtggaagttctccgcagtaaagtattacgtgatgcaattgagtccacaggagatttcaaagcttttgaagcaacaagacaagccatggatgaagttatgaaggcccttaaagatgacgaggtcactgccattggtgtctacggcatgggaggcgttggaaagacaaccatggtaagacatgtcggtgcacaagccctaaaaaaggggatttttcttcatgtgattatggcagtcgtatcccaaagccctaacttgaaaaaaattcaaggaacattagcagaactgttgggattcgaattgaagaagaagacagaaattggaagagcagctaaattgaggatggagttaatgagaagaacaaagatccttataatcctggacgatatttgggagagaatagacatatcaagaataggaatacctagctacaaggaacttcaaatgtgcaattccaaagtactactcaccacaaggataaggaatgtctgtcatgtcatgagatgccaaagaacgatcaccctcagtatcctatcagaagatgattcttggaccttatttgtgagaaatgcaggaaggtctcttgaatccaccaattttgaggatgtagcgaggagggtagctagagaatgctgtggtctaccgattgcattgataacagttgcaagggcactcggagataaagacttggtggaatggaaaagagcagttcaacgactagagaagtcgcaaactgccaaccctgatgattacggagaagcatccaaatgtataaaattaagctatgattacttgaaagatgaggactacaagtcatacttcttgctctgttgcttattcccagaagactatggaatcgaaatagaagacttgttcaggtatgcgataggtaaaggattgtttcgagatgccgacacacttgaagaagccagagagagagctgattccgtggctaggcaccttaattattcttgcttgcttttggtgacagaaagtaggagagaaagaattaaatgtgtaagcatgcatgatgttgtccgggatacagccattcagattgtgcaatctgaacatgggtttttggtgagagctggctgtgatttaaaggactggccacgtcaattacatgaaggctattctgcaatctcactaatgcagaataatatttgcaagctacctgaaaaattggtatgtccagaactccagattttgttactagactctaatttatctatggtagagatcccagaaacattcttccaaggtccaaataaattaacggttttagatctttcgaacactggcatcacattactacccgaatcgttcagtctcctgatcaacctccgtgctttgtgtttagatagatgccgtaaccttgacacttacatactaggaaaattgaaaaagcttgagattcttagcatgagatactgttgtgtaaagaagttgtcgagagagatgggaaatttgatcaatctaaggatgttggatatcactgatgcagttattgacacaattccatcgaaagtgatatcaaagtttcataatttagaagaactgtacatgcaaggattttggaactgggggagtaaagttgagggacaaggagaagaaaccaatattagctttgacgaggtaactagcttgtcaaatttaagagttttgaaggtttatatctccaaagcagaacttatccctaaatatgttgatttcaaaccgaattgggtcgagtttgatatacgtatcggtacagacgacaggcagagaatctttagaccatatggtcataaatcattttccaagaagcttgcgattgccaccatcagtggcttaccgaactggttttgcaacgcgctgatgaacactgcagagaggctatggtgggaaggccgccaaaattttggtgacatatttgtggaatatgaattgatgaacacaatacgggttccaaaagaacctgtgttcgagaacttggaagagttgcatctgtatttcgtgaatggcctagagttacgtgttggtgaattgccacctgggtctctatttaatttgaagttattggaggtaaaattttgttacaattgggggaatgtatttttaccatcaaatttgttactaccaaacctagaagaactaaattgtcgtttcacgggcgggattgaatatgtgtttggttgtgaagcgttatttgagccaaaacaatcaaaattgagaaagatagagttgaaccatctaacgacagcaagaagcatatgtcatggacctgctccagctgcaatgtttcaggctcttcaaagtttgaccattcggcggtgcaacttgcagggaagtctcttcacctctgatgttgctcaatgtctttctcagttgaattctcttgaactacacgattgcccctttttggaaagaatagttgaagcaagcaacaagaagatcattcttccaaaattgaggcgattgactttgttggtacttccaatgttgtcccatgaaagtgctacttttgagtgtccttcactggtatatttggacttgtggggctgccccaacttttccgcctctttttccgacttccacacctactattag

Protein Analysis

1143

Amino Acids

131.09

Weight (kDa)

7.93

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 813, 1214
AasI GACNNNNNNGTC 1 cut(s) 2625
AatII GACGTC 1 cut(s) 146
Acc36I ACCTGC 2 cut(s) 75, 3115
AccB1I GGYRCC 1 cut(s) 1797
AccI GTMKAC 6 cut(s) 240, 265, 399, 730, 981, 1480
AccII CGCG 2 cut(s) 104, 2696
AciI CCGC 5 cut(s) 856, 2734, 2997, 3154, 3399
AclWI GGATC 2 cut(s) 1201, 2077
AcoI YGGCCR 1 cut(s) 1952
AcvI CACGTG 1 cut(s) 655
AcyI GRCGYC 1 cut(s) 143
AfaI GTAC 9 cut(s) 215, 260, 321, 334, 773, 1302, 2405, 2593, 3315
AfiI CCNNNNNNNGG 6 cut(s) 55, 342, 1045, 1794, 3153, 3244
AflIII ACRYGT 2 cut(s) 1021, 2857
AhdI GACNNNNNGTC 1 cut(s) 1651
AjiI CACGTC 1 cut(s) 1958
AjnI CCWGG 2 cut(s) 1218, 2875
AjuI GAANNNNNNNTTGG 2 cut(s) 2997, 3029
Alw21I GWGCWC 1 cut(s) 1033
Alw26I GTCTC 5 cut(s) 134, 1425, 2177, 2886, 3179
Alw44I GTGCAC 1 cut(s) 1029
AlwI GGATC 2 cut(s) 1201, 2077
AoxI GGCC 9 cut(s) 46, 328, 348, 360, 376, 945, 1952, 2731, 2845
ApaLI GTGCAC 1 cut(s) 1029
ApeKI GCWGC 4 cut(s) 518, 1169, 3116, 3384
AspLEI GCGC 1 cut(s) 2698
AspS9I GGNCC 8 cut(s) 46, 328, 361, 376, 946, 1392, 2105, 3104
AsuC2I CCSGG 1 cut(s) 1880
AsuHPI GGTGA 9 cut(s) 76, 760, 1300, 1351, 1837, 1936, 2759, 2876, 3172
AsuII TTCGAA 5 cut(s) 188, 384, 696, 1137, 2135
AvaII GGWCC 3 cut(s) 1392, 2105, 3104
BaeGI GKGCMC 2 cut(s) 1033, 1513
BaeI ACNNNNGTAYC 2 cut(s) 2263, 2296
BalI TGGCCA 1 cut(s) 1954
BanI GGYRCC 1 cut(s) 1797
BanII GRGCYC 1 cut(s) 663
BbrPI CACGTG 1 cut(s) 655
BbsI GAAGAC 3 cut(s) 1157, 1692, 1713
Bbv12I GWGCWC 1 cut(s) 1033
BbvCI CCTCAGC 1 cut(s) 125
BbvI GCAGC 4 cut(s) 530, 1181, 3103, 3371
BccI CCATC 7 cut(s) 142, 1177, 2296, 2371, 2672, 2953, 3081
BceAI ACGGC 3 cut(s) 315, 1000, 2196
BcgI CGANNNNNNTGC 2 cut(s) 950, 984
BciT130I CCWGG 2 cut(s) 1220, 2877
BciVI GTATCC 3 cut(s) 1090, 1379, 1876
BclI TGATCA 2 cut(s) 2178, 2313
BcnI CCSGG 1 cut(s) 1880
BcoDI GTCTC 5 cut(s) 134, 1425, 2177, 2886, 3179
BfmI CTRYAG 1 cut(s) 2709
BfuAI ACCTGC 2 cut(s) 75, 3115
BfuI GTATCC 3 cut(s) 1090, 1379, 1876
BglII AGATCT 2 cut(s) 603, 2128
BisI GCNGC 5 cut(s) 519, 1170, 2734, 3117, 3385
BlsI GCNGC 5 cut(s) 520, 1171, 2735, 3118, 3386
BmcAI AGTACT 1 cut(s) 1302
Bme1390I CCNGG 3 cut(s) 1220, 1880, 2877
Bme18I GGWCC 3 cut(s) 1392, 2105, 3104
BmeRI GACNNNNNGTC 1 cut(s) 1651
BmgBI CACGTC 1 cut(s) 1958
BmgT120I GGNCC 8 cut(s) 46, 328, 361, 376, 946, 1392, 2105, 3104
BmiI GGNNCC 5 cut(s) 47, 158, 660, 1799, 2790
BmrFI CCNGG 3 cut(s) 1220, 1880, 2877
BmrI ACTGGG 1 cut(s) 2434
BmsI GCATC 9 cut(s) 754, 865, 1334, 1610, 1738, 2154, 2198, 2332, 2836
BmuI ACTGGG 1 cut(s) 2434
BpiI GAAGAC 3 cut(s) 1157, 1692, 1713
BpmI CTGGAG 2 cut(s) 2027, 3096
Bpu10I CCTNAGC 1 cut(s) 125
Bpu14I TTCGAA 5 cut(s) 188, 384, 696, 1137, 2135
BpuEI CTTGAG 1 cut(s) 2273
BpuMI CCSGG 1 cut(s) 1880
BsaAI YACGTR 4 cut(s) 655, 872, 2585, 2858
BsaHI GRCGYC 1 cut(s) 143
BsaI GGTCTC 2 cut(s) 1425, 2886
BsaJI CCNNGG 5 cut(s) 927, 1011, 1788, 2101, 2876
Bsc4I CCNNNNNNNGG 6 cut(s) 55, 342, 1045, 1794, 3153, 3244
Bse118I RCCGGY 1 cut(s) 378
Bse1I ACTGG 5 cut(s) 1955, 2146, 2429, 2687, 3366
Bse3DI GCAATG 1 cut(s) 3126
BseBI CCWGG 2 cut(s) 1220, 2877
BseDI CCNNGG 5 cut(s) 927, 1011, 1788, 2101, 2876
BseGI GGATG 9 cut(s) 184, 475, 499, 769, 937, 1188, 1450, 1601, 2331
BseLI CCNNNNNNNGG 6 cut(s) 55, 342, 1045, 1794, 3153, 3244
BseMI GCAATG 1 cut(s) 3126
BseMII CTCAG 3 cut(s) 116, 1378, 3221
BseNI ACTGG 5 cut(s) 1955, 2146, 2429, 2687, 3366
BseRI GAGGAG 3 cut(s) 188, 703, 1468
BseSI GKGCMC 2 cut(s) 1033, 1513
BseXI GCAGC 4 cut(s) 530, 1181, 3103, 3371
Bsh1236I CGCG 2 cut(s) 104, 2696
Bsh1285I CGRYCG 2 cut(s) 256, 436
BshFI GGCC 9 cut(s) 48, 330, 350, 362, 378, 947, 1954, 2733, 2847
BshNI GGYRCC 1 cut(s) 1797
BsiEI CGRYCG 2 cut(s) 256, 436
BsiHKAI GWGCWC 1 cut(s) 1033
BsiSI CCGG 3 cut(s) 49, 379, 1879
BslFI GGGAC 2 cut(s) 2457, 3313
BslI CCNNNNNNNGG 6 cut(s) 55, 342, 1045, 1794, 3153, 3244
BsmAI GTCTC 5 cut(s) 134, 1425, 2177, 2886, 3179
BsmBI CGTCTC 1 cut(s) 134
BsmFI GGGAC 2 cut(s) 2457, 3313
BsmI GAATGC 1 cut(s) 1475
BsnI GGCC 9 cut(s) 48, 330, 350, 362, 378, 947, 1954, 2733, 2847
Bso31I GGTCTC 2 cut(s) 1425, 2886
Bsp119I TTCGAA 5 cut(s) 188, 384, 696, 1137, 2135
Bsp1286I GDGCHC 3 cut(s) 663, 1033, 1513
Bsp1407I TGTACA 3 cut(s) 213, 771, 2403
Bsp143I GATC 9 cut(s) 433, 603, 1206, 1356, 2082, 2128, 2178, 2313, 3276
Bsp19I CCATGG 2 cut(s) 927, 1011
BspACI CCGC 5 cut(s) 856, 2734, 2997, 3154, 3399
BspANI GGCC 9 cut(s) 48, 330, 350, 362, 378, 947, 1954, 2733, 2847
BspCNI CTCAG 3 cut(s) 117, 1377, 3220
BspFNI CGCG 2 cut(s) 104, 2696
BspHI TCATGA 1 cut(s) 1338
BspLI GGNNCC 5 cut(s) 47, 158, 660, 1799, 2790
BspMAI CTGCAG 1 cut(s) 2713
BspMI ACCTGC 2 cut(s) 75, 3115
BspPI GGATC 2 cut(s) 1201, 2077
BspQI GCTCTTC 2 cut(s) 1159, 3138
BspT104I TTCGAA 5 cut(s) 188, 384, 696, 1137, 2135
BspT107I GGYRCC 1 cut(s) 1797
BspTNI GGTCTC 2 cut(s) 1425, 2886
BsrDI GCAATG 1 cut(s) 3126
BsrFI RCCGGY 1 cut(s) 378
BsrGI TGTACA 3 cut(s) 213, 771, 2403
BsrI ACTGG 5 cut(s) 1955, 2146, 2429, 2687, 3366
BssAI RCCGGY 1 cut(s) 378
BssECI CCNNGG 5 cut(s) 927, 1011, 1788, 2101, 2876
BssMI GATC 9 cut(s) 433, 603, 1206, 1356, 2082, 2128, 2178, 2313, 3276
BssNAI GTATAC 2 cut(s) 241, 266
BssNI GRCGYC 1 cut(s) 143
BssT1I CCWWGG 3 cut(s) 927, 1011, 2101
Bst1107I GTATAC 2 cut(s) 241, 266
Bst2UI CCWGG 2 cut(s) 1220, 2877
Bst6I CTCTTC 6 cut(s) 104, 680, 1159, 2814, 3138, 3182
BstACI GRCGYC 1 cut(s) 143
BstAUI TGTACA 3 cut(s) 213, 771, 2403
BstBAI YACGTR 4 cut(s) 655, 872, 2585, 2858
BstBI TTCGAA 5 cut(s) 188, 384, 696, 1137, 2135
BstC8I GCNNGC 7 cut(s) 348, 1817, 1865, 1933, 2012, 2652, 3265
BstDEI CTNAG 8 cut(s) 125, 514, 596, 711, 1364, 2261, 2321, 3207
BstDSI CCRYGG 3 cut(s) 927, 1011, 1788
BstENI CCTNNNNNAGG 1 cut(s) 1043
BstF5I GGATG 9 cut(s) 184, 475, 499, 769, 937, 1188, 1450, 1601, 2331
BstFNI CGCG 2 cut(s) 104, 2696
BstHHI GCGC 1 cut(s) 2698
BstKTI GATC 9 cut(s) 436, 606, 1209, 1359, 2085, 2131, 2181, 2316, 3279
BstMAI GTCTC 5 cut(s) 134, 1425, 2177, 2886, 3179
BstMBI GATC 9 cut(s) 433, 603, 1206, 1356, 2082, 2128, 2178, 2313, 3276
BstMCI CGRYCG 2 cut(s) 256, 436
BstMWI GCNNNNNNNGC 3 cut(s) 911, 993, 3116
BstNI CCWGG 2 cut(s) 1220, 2877
BstNSI RCATGY 3 cut(s) 1025, 1867, 2410
BstSCI CCNGG 3 cut(s) 1218, 1878, 2875
BstSFI CTRYAG 1 cut(s) 2709
BstSLI GKGCMC 2 cut(s) 1033, 1513
BstSNI TACGTA 1 cut(s) 2585
BstUI CGCG 2 cut(s) 104, 2696
BstV1I GCAGC 4 cut(s) 530, 1181, 3103, 3371
BstV2I GAAGAC 3 cut(s) 1157, 1692, 1713
BstX2I RGATCY 4 cut(s) 603, 1206, 2082, 2128
BstXI CCANNNNNNTGG 1 cut(s) 2744
BstYI RGATCY 4 cut(s) 603, 1206, 2082, 2128
BstZ17I GTATAC 2 cut(s) 241, 266
BsuI GTATCC 3 cut(s) 1090, 1379, 1876
BsuRI GGCC 9 cut(s) 48, 330, 350, 362, 378, 947, 1954, 2733, 2847
BtgI CCRYGG 3 cut(s) 927, 1011, 1788
BtrI CACGTC 1 cut(s) 1958
BtsCI GGATG 9 cut(s) 184, 475, 499, 769, 937, 1188, 1450, 1601, 2331
BtsI GCAGTG 3 cut(s) 761, 966, 2706
BtsIMutI CAGTG 7 cut(s) 761, 966, 2139, 2337, 2674, 2706, 3359
BveI ACCTGC 2 cut(s) 75, 3115
Cac8I GCNNGC 7 cut(s) 348, 1817, 1865, 1933, 2012, 2652, 3265
CciI TCATGA 1 cut(s) 1338
CfoI GCGC 1 cut(s) 2698
Cfr10I RCCGGY 1 cut(s) 378
Cfr13I GGNCC 8 cut(s) 46, 328, 361, 376, 946, 1392, 2105, 3104
Csp6I GTAC 9 cut(s) 214, 259, 320, 333, 772, 1301, 2404, 2592, 3314
CspCI CAANNNNNGTGG 2 cut(s) 1420, 1455
CviQI GTAC 9 cut(s) 214, 259, 320, 333, 772, 1301, 2404, 2592, 3314
DdeI CTNAG 8 cut(s) 125, 514, 596, 711, 1364, 2261, 2321, 3207
DpnI GATC 9 cut(s) 435, 605, 1208, 1358, 2084, 2130, 2180, 2315, 3278
DpnII GATC 9 cut(s) 433, 603, 1206, 1356, 2082, 2128, 2178, 2313, 3276
DraI TTTAAA 1 cut(s) 1944
DrdI GACNNNNNNGTC 1 cut(s) 2625
DriI GACNNNNNGTC 1 cut(s) 1651
DseDI GACNNNNNNGTC 1 cut(s) 2625
EaeI YGGCCR 1 cut(s) 1952
Eam1104I CTCTTC 6 cut(s) 104, 680, 1159, 2814, 3138, 3182
Eam1105I GACNNNNNGTC 1 cut(s) 1651
EarI CTCTTC 6 cut(s) 104, 680, 1159, 2814, 3138, 3182
EciI GGCGGA 1 cut(s) 3388
Eco105I TACGTA 1 cut(s) 2585
Eco130I CCWWGG 3 cut(s) 927, 1011, 2101
Eco24I GRGCYC 1 cut(s) 663
Eco31I GGTCTC 2 cut(s) 1425, 2886
Eco32I GATATC 3 cut(s) 677, 2335, 2376
Eco47I GGWCC 3 cut(s) 1392, 2105, 3104
Eco72I CACGTG 1 cut(s) 655
EcoNI CCTNNNNNAGG 1 cut(s) 1043
EcoO109I RGGNCCY 1 cut(s) 946
EcoRI GAATTC 1 cut(s) 3214
EcoRII CCWGG 2 cut(s) 1218, 2875
EcoRV GATATC 3 cut(s) 677, 2335, 2376
EcoT14I CCWWGG 3 cut(s) 927, 1011, 2101
EcoT22I ATGCAT 1 cut(s) 1869
EcoT38I GRGCYC 1 cut(s) 663
ErhI CCWWGG 3 cut(s) 927, 1011, 2101
Esp3I CGTCTC 1 cut(s) 134
FalI AAGNNNNNCTT 6 cut(s) 1643, 1675, 2517, 2549, 3328, 3360
FaqI GGGAC 2 cut(s) 2457, 3313
FauI CCCGC 1 cut(s) 2990
FauNDI CATATG 2 cut(s) 2623, 3095
FbaI TGATCA 2 cut(s) 2178, 2313
FblI GTMKAC 6 cut(s) 240, 265, 399, 730, 981, 1480
Fnu4HI GCNGC 5 cut(s) 519, 1170, 2734, 3117, 3385
FokI GGATG 9 cut(s) 191, 482, 506, 776, 944, 1195, 1457, 1588, 2338
FriOI GRGCYC 1 cut(s) 663
Fsp4HI GCNGC 5 cut(s) 519, 1170, 2734, 3117, 3385
GlaI GCGC 1 cut(s) 2697
GluI GCNGC 5 cut(s) 519, 1170, 2734, 3117, 3385
GsuI CTGGAG 2 cut(s) 2027, 3096
HaeIII GGCC 9 cut(s) 48, 330, 350, 362, 378, 947, 1954, 2733, 2847
HapII CCGG 3 cut(s) 49, 379, 1879
HhaI GCGC 1 cut(s) 2698
Hin1I GRCGYC 1 cut(s) 143
Hin6I GCGC 1 cut(s) 2696
HinP1I GCGC 1 cut(s) 2696
HincII GTYRAC 2 cut(s) 731, 835
HindII GTYRAC 2 cut(s) 731, 835
HindIII AAGCTT 4 cut(s) 702, 903, 2249, 2648
HpaII CCGG 3 cut(s) 49, 379, 1879
HphI GGTGA 9 cut(s) 76, 760, 1300, 1351, 1837, 1936, 2759, 2876, 3172
Hpy166II GTNNAC 9 cut(s) 241, 266, 400, 731, 835, 888, 982, 1031, 1481
Hpy188I TCNGA 9 cut(s) 667, 681, 808, 1378, 1517, 1896, 1909, 3187, 3412
Hpy8I GTNNAC 9 cut(s) 241, 266, 400, 731, 835, 888, 982, 1031, 1481
Hpy99I CGWCG 2 cut(s) 148, 274
HpyAV CCTTC 9 cut(s) 299, 491, 739, 937, 1410, 1964, 2503, 2723, 3366
HpyCH4IV ACGT 6 cut(s) 143, 654, 871, 1957, 2584, 2857
HpyF10VI GCNNNNNNNGC 3 cut(s) 911, 993, 3116
HpyF3I CTNAG 8 cut(s) 125, 514, 596, 711, 1364, 2261, 2321, 3207
HpySE526I ACGT 6 cut(s) 143, 654, 871, 1957, 2584, 2857
Hsp92I GRCGYC 1 cut(s) 143
HspAI GCGC 1 cut(s) 2696
Ksp22I TGATCA 2 cut(s) 2178, 2313
Kzo9I GATC 9 cut(s) 433, 603, 1206, 1356, 2082, 2128, 2178, 2313, 3276
LguI GCTCTTC 2 cut(s) 1159, 3138
LmnI GCTCC 3 cut(s) 156, 658, 3115
Lsp1109I GCAGC 4 cut(s) 530, 1181, 3103, 3371
LweI GCATC 9 cut(s) 754, 865, 1334, 1610, 1738, 2154, 2198, 2332, 2836
MaeII ACGT 6 cut(s) 143, 654, 871, 1957, 2584, 2857
MaeIII GTNAC 9 cut(s) 964, 1825, 2052, 2213, 2479, 2747, 2853, 2918, 2955
MalI GATC 9 cut(s) 435, 605, 1208, 1358, 2084, 2130, 2180, 2315, 3278
MboI GATC 9 cut(s) 433, 603, 1206, 1356, 2082, 2128, 2178, 2313, 3276
MfeI CAATTG 3 cut(s) 543, 879, 2923
MflI RGATCY 4 cut(s) 603, 1206, 2082, 2128
MhlI GDGCHC 3 cut(s) 663, 1033, 1513
MlsI TGGCCA 1 cut(s) 1954
MluNI TGGCCA 1 cut(s) 1954
MlyI GAGTC 4 cut(s) 171, 811, 893, 2054
MmeI TCCRAC 2 cut(s) 979, 2310
Mox20I TGGCCA 1 cut(s) 1954
Mph1103I ATGCAT 1 cut(s) 1869
MscI TGGCCA 1 cut(s) 1954
MslI CAYNNNNRTG 5 cut(s) 30, 1026, 1606, 2756, 3336
Msp20I TGGCCA 1 cut(s) 1954
MspA1I CMGCKG 1 cut(s) 3116
MspI CCGG 3 cut(s) 49, 379, 1879
MspR9I CCNGG 3 cut(s) 1220, 1880, 2877
MunI CAATTG 3 cut(s) 543, 879, 2923
Mva1269I GAATGC 1 cut(s) 1475
MvaI CCWGG 2 cut(s) 1220, 2877
MvnI CGCG 2 cut(s) 104, 2696
MwoI GCNNNNNNNGC 3 cut(s) 911, 993, 3116
NciI CCSGG 1 cut(s) 1880
NcoI CCATGG 2 cut(s) 927, 1011
NdeI CATATG 2 cut(s) 2623, 3095
NdeII GATC 9 cut(s) 433, 603, 1206, 1356, 2082, 2128, 2178, 2313, 3276
NlaIV GGNNCC 5 cut(s) 47, 158, 660, 1799, 2790
NmeAIII GCCGAG 3 cut(s) 185, 323, 376
NmuCI GTSAC 3 cut(s) 964, 1825, 2747
NsiI ATGCAT 1 cut(s) 1869
NspI RCATGY 3 cut(s) 1025, 1867, 2410
NspV TTCGAA 5 cut(s) 188, 384, 696, 1137, 2135
PaeI GCATGC 1 cut(s) 1867
PagI TCATGA 1 cut(s) 1338
PciI ACATGT 1 cut(s) 1021
PciSI GCTCTTC 2 cut(s) 1159, 3138
PcsI WCGNNNNNNNCGW 3 cut(s) 101, 328, 2595
PctI GAATGC 1 cut(s) 1475
PflFI GACNNNGTC 1 cut(s) 962
PfoI TCCNGGA 2 cut(s) 1218, 1878
PkrI GCNGC 5 cut(s) 520, 1171, 2735, 3118, 3386
Ple19I CGATCG 1 cut(s) 436
PleI GAGTC 4 cut(s) 170, 811, 892, 2054
PmaCI CACGTG 1 cut(s) 655
PmlI CACGTG 1 cut(s) 655
PpsI GAGTC 4 cut(s) 170, 811, 892, 2054
Ppu21I YACGTR 4 cut(s) 655, 872, 2585, 2858
PscI ACATGT 1 cut(s) 1021
PsiI TTATAA 2 cut(s) 813, 1214
Psp6I CCWGG 2 cut(s) 1218, 2875
PspCI CACGTG 1 cut(s) 655
PspGI CCWGG 2 cut(s) 1218, 2875
PspN4I GGNNCC 5 cut(s) 47, 158, 660, 1799, 2790
PspPI GGNCC 8 cut(s) 46, 328, 361, 376, 946, 1392, 2105, 3104
PstI CTGCAG 1 cut(s) 2713
PsuI RGATCY 4 cut(s) 603, 1206, 2082, 2128
PsyI GACNNNGTC 1 cut(s) 962
PvuI CGATCG 1 cut(s) 436
PvuII CAGCTG 1 cut(s) 3116
RsaI GTAC 9 cut(s) 215, 260, 321, 334, 773, 1302, 2405, 2593, 3315
RsaNI GTAC 9 cut(s) 214, 259, 320, 333, 772, 1301, 2404, 2592, 3314
RseI CAYNNNNRTG 5 cut(s) 30, 1026, 1606, 2756, 3336
SalI GTCGAC 1 cut(s) 729
SapI GCTCTTC 2 cut(s) 1159, 3138
SatI GCNGC 5 cut(s) 519, 1170, 2734, 3117, 3385
Sau3AI GATC 9 cut(s) 433, 603, 1206, 1356, 2082, 2128, 2178, 2313, 3276
Sau96I GGNCC 8 cut(s) 46, 328, 361, 376, 946, 1392, 2105, 3104
ScaI AGTACT 1 cut(s) 1302
SchI GAGTC 4 cut(s) 171, 811, 893, 2054
ScrFI CCNGG 3 cut(s) 1220, 1880, 2877
SduI GDGCHC 3 cut(s) 663, 1033, 1513
SfaNI GCATC 9 cut(s) 754, 865, 1334, 1610, 1738, 2154, 2198, 2332, 2836
SfcI CTRYAG 1 cut(s) 2709
SfuI TTCGAA 5 cut(s) 188, 384, 696, 1137, 2135
SinI GGWCC 3 cut(s) 1392, 2105, 3104
SmiMI CAYNNNNRTG 5 cut(s) 30, 1026, 1606, 2756, 3336
SmlI CTYRAG 1 cut(s) 2252
SmoI CTYRAG 1 cut(s) 2252
SnaBI TACGTA 1 cut(s) 2585
SphI GCATGC 1 cut(s) 1867
SsiI CCGC 5 cut(s) 856, 2734, 2997, 3154, 3399
SspI AATATT 2 cut(s) 2005, 2464
StyD4I CCNGG 3 cut(s) 1218, 1878, 2875
StyI CCWWGG 3 cut(s) 927, 1011, 2101
TaiI ACGT 6 cut(s) 146, 657, 874, 1960, 2587, 2860
TaqII GACCGA 1 cut(s) 242
TatI WGTACW 4 cut(s) 213, 771, 1300, 2403
TauI GCSGC 1 cut(s) 2736
TscAI CASTG 7 cut(s) 761, 973, 2146, 2344, 2674, 2713, 3366
TseFI GTSAC 3 cut(s) 964, 1825, 2747
TseI GCWGC 4 cut(s) 518, 1169, 3116, 3384
Tsp45I GTSAC 3 cut(s) 964, 1825, 2747
TspGWI ACGGA 3 cut(s) 1608, 1777, 2177
TspRI CASTG 7 cut(s) 761, 973, 2146, 2344, 2674, 2713, 3366
Tth111I GACNNNGTC 1 cut(s) 962
VneI GTGCAC 1 cut(s) 1029
VpaK11BI GGWCC 3 cut(s) 1392, 2105, 3104
XagI CCTNNNNNAGG 1 cut(s) 1043
XceI RCATGY 3 cut(s) 1025, 1867, 2410
XmiI GTMKAC 6 cut(s) 240, 265, 399, 730, 981, 1480
ZraI GACGTC 1 cut(s) 144
ZrmI AGTACT 1 cut(s) 1302
Zsp2I ATGCAT 1 cut(s) 1869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.