Rmu_sc0001208.1_g000087

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001208.1
OG0000085
Physical Location & Seq
Reverse (-)
370418 .. 376385
5968 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001208.1_g000087.1.cds

Sequence Viewer

Length: 4254 bp
atgtggcctgatatgtggatcccaaaaccttatagctttaaaccatattcgcctgttatgcaagggactaaggatttatatgtgtctgacctaattgatttagagaagggacaatgggagctgtggcttttaaacgagttgttcatggatgatgaggtgcagagtattgcacaaatccctctaagtctggatggaggtgaggatagattactatggcattttgacaagaaaggaatctacagtatgaggaatggttaccacttaacaaggtttttggagcacttggatactaatggatccagtagtgggacagggattggcggtgacaaattttggaagaggctttggaaaataaatgtgcccccaaaggctagaatgcaagcttggcatctggttaagggtttcttgtccacgaaaatagccctaggcctttgcagcttaaaccgcccggtaatgcatcctgcacgcactattgatgattgggtcaaagaaatgagtggaactctaaatgagtcagcattacataccgaggtggaagctcttcgggctggtcttttggtggctgttcatcaaggttgggatgaggttgaaattgagttagattgcgcaacacttgttaatgcccttgatatgggtttggaggacctgactgatataggccggattatagaagactgcaaaaggcttgcacatcttgctagttggggtaatttggatgagttttggattgaagagacgcccgttattattctggatgttctttatgaggatcaatgtaatgctttaaggggtttaagcttgatgtctcccccatttcttaagtggatatggaagaattggttcattattgtggtgacatggaacgtaatagccagtcttgggtggatatggaagagtaggttcagttgcatggttgaaaccccagagccaatatcagaggataatcaagaactggttgacgtcgatgcctctgcatctcttccttcatcatcagctcctaggtggaagcatgatgtctttttgagtttcaggggtgaggacactcgtaggggtattacatcctttttatacgatcgactgaaaaacagaggaatcaaaacattcatggatggccacgaccttcaagtaggggatgttatttctcccactctcctgatggcaattaaagaatcaaggtttgcaattattgttctctctccaaactatgcctcttccacttggtgtttggaggaacttagaaacatttgtgaacgcatgaaagaagacaacagtagaattctgccacttttttataatgtggatcccagtgatgtacgaaatcagaagaggagttttggagatgctttcactaagcatgaaaaatctgggaaacacaaatcaggaaaggtgcagcagtggagagaagctttaaaaaaagtggcaaatttctctggatggcatacactgaattataaaactgatagagaacttgtggatgccattgaggaatctgtgtgcaataaattacgactgactgaacctgtgcttaaaatgtgcatcggaggttttaaagaatttgcagcaacaaaacaagccattgataaggtcatgaacgcgctaaaagatgatgaggccactaccattggagtctacggaatggggggcgttggaaagacgacaatggtgaaatatgttggagcacaagcccaaaaaagtaggctttttaatcaagtgataatggctgtcgtatcccaaaaccccgacttgatgaaaattcaacagacatttgcagaaatgctgggcttgaaattggaggagaagacagaaattgtaagagccagtaaattgaaggagaagatattgggagaaactgggatcctcataatcttggatgacatttggaagagcatagacttttcaagcattggaattccaagccacaacgaacttcaaagatgcaattccaaagttctactgactaccagaaaattgactgtttgccattccatggattgccatgcaaaagtacatctcaatatcttatcagaagaagattcttggagcttatttgtgaaggaagcaaggaagtaccctcttgacaaatcatccactttctatgatgtggcacgtaaggtggctagagaatgcgccggtctaccacttgctttgatagcagtcgcgagggcccttcgaggtcaaggtttggatggatggaaagaagctgctcgacaactacaagcgtctcaacctcccaaccctgaagatgagggagttgtgtatgcatccataaagttaagctatgattacttgaaatctgatgattccaaatcatgcttcttgctttgctgcctgttcccagaagattatgatatcccaattgaatacttgctcatgtatggaattgggaaaggaatgtttcaagattccaacatgctagaagcccgagacagaacatatttagtggtgaaggcccttaaagattctagcttgcttttggatgctagagatgacggatgtgtaaggatgcatgatgtcattcgggacgtggcaatatcaatttcattatctgaagacggccagcggttcttggtgaaaagtagctatgaattgaagaactggccgaagattgatgcacataaaggctactctgcaatctcactaatgaagaacgaaatttgcaagctaccggaagagttggtatgtccaaatctccagattttattactacaacacaatgcttctttcaatgagatcccaaaatcatttttccagagtcagaatgaattaagagtcttggatcttagctacactcgtatttcattactaccccaatcaatcagttctctaaccaaccttcaagctttgtatttagattattgctggaacataaatgacatttctgtaatcggaaaacttcacaagcttgagattcttagtatgagaacatttgctctggaagaattgtcaagagaaataggacagttgaccaatctaaggatgctggatgtcagtgctgtatatattcgcacaatcccatctaaagtgatatccaagttgcataagttggaagaactgtacatggaatgtggatttttgaactgggggagtaaaattgatggagaaggagaagaaactaatattggctttgatgaattagctagtttattatatttaagcattttgacagtttgcatatcggatgcaaatggcatccctacaagtgttgaggtcgaaccgaattgggtttacttcgatatcagtattcgcagcggccatagtggagggagagtcattaaaaaacttcgatccggttgtaattgtagatccttgagcctttatagaactgacacaaccataggaacctttccggactggtttgtcaatgctgtggtaaagaatactgagaggctgcgttataaagaatgcaaagggttacgtaacattcttgtggaatatgaccgtgggaggtttcatggactgaaagttctctctgtaattggtcagtgtgcgaacttgaaagaactgatgaatgcaataacatgtgttccacatatgcctgtgtttgagaacttggaagagttgtatctggaagacctgtgtcatctgaggcagttatgtgttggtgagttaccacctggatctctctgcagtctgaaattattgaaggtctgtatgtgtcctatttttgggaatgcactgttgccatcaaaactgttgcagaaactagcaaatctggaaaaactaaactgtgagacaagtaaagtgaaacatgtttttggatgtgaaggatttgagctagatcatacaaatctgagagagatggtgttgttggatctacccgcagtaagaagcatgtgtaatggtcctgctccacgtggaatgttccggactctaaagaaattgttcattatgaaatgcaacttccggggaagtctcttcacatttgatgtagctcagtgtctttttcaattggaaaaccttgatgtaaccatctgccctgtcttggaaagagtaatcgaagcaaggagggaaacattgaacaacaaggagaccattcttccaaaattgaagtacttatgtttggaatatcttccaatgttgtacaagggaaatgctactattgattttgagtgtccttcattggaatacttgcggatgaaggaatgcccccacttgtcatttccatcctctgtttctgactacttcaacagcaggaatcaagttcttttcaatgatggtcgttttatgaatcggatgcgcagatactccagatag

Protein Analysis

1417

Amino Acids

161.45

Weight (kDa)

6.35

Isoelectric Point (pI)

41.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1293, 1452, 3396
AasI GACNNNNNNGTC 1 cut(s) 3356
AatII GACGTC 1 cut(s) 963
Acc16I TGCGCA 2 cut(s) 607, 4238
AccB7I CCANNNNNTGG 1 cut(s) 1987
AccI GTMKAC 2 cut(s) 1629, 2134
AccII CGCG 2 cut(s) 1595, 2159
AccIII TCCGGA 2 cut(s) 3346, 3864
AciI CCGC 6 cut(s) 321, 445, 2560, 3249, 3819, 4132
AcoI YGGCCR 4 cut(s) 1111, 2554, 2597, 3250
AcsI RAATTY 7 cut(s) 329, 1275, 1423, 1553, 1752, 1908, 2652
AcuI CTGAAG 2 cut(s) 2259, 2568
AcvI CACGTG 1 cut(s) 3854
AcyI GRCGYC 2 cut(s) 737, 960
AdeI CACNNNGTG 1 cut(s) 1221
AfaI GTAC 6 cut(s) 1314, 2007, 2069, 3056, 4052, 4082
AfiI CCNNNNNNNGG 7 cut(s) 306, 631, 879, 1987, 2973, 3665, 3982
AflII CTTAAG 1 cut(s) 818
AflIII ACRYGT 2 cut(s) 3518, 3748
AjiI CACGTC 1 cut(s) 2524
AjnI CCWGG 1 cut(s) 3613
AjuI GAANNNNNNNTTGG 6 cut(s) 367, 399, 2297, 2329, 3102, 3134
AloI GAACNNNNNNTCC 2 cut(s) 3447, 3479
Alw21I GWGCWC 2 cut(s) 282, 1681
Alw26I GTCTC 7 cut(s) 728, 810, 2226, 2418, 3725, 3917, 4022
Ama87I CYCGRG 1 cut(s) 2421
Aor13HI TCCGGA 2 cut(s) 3346, 3864
ApaI GGGCCC 1 cut(s) 2167
ApeKI GCWGC 7 cut(s) 435, 1390, 1559, 2201, 2325, 3246, 3388
ApoI RAATTY 7 cut(s) 329, 1275, 1423, 1553, 1752, 1908, 2652
Asp700I GAANNNNTTC 4 cut(s) 540, 839, 3881, 4068
AspA2I CCTAGG 2 cut(s) 424, 998
AspLEI GCGC 4 cut(s) 608, 1597, 2129, 4239
AspS9I GGNCC 5 cut(s) 643, 2163, 2164, 2449, 3842
AsuC2I CCSGG 2 cut(s) 449, 3905
AsuHPI GGTGA 8 cut(s) 209, 335, 865, 1046, 1675, 2455, 2581, 3614
AvaI CYCGRG 1 cut(s) 2421
AvaII GGWCC 2 cut(s) 643, 3842
AvrII CCTAGG 2 cut(s) 424, 998
BaeGI GKGCMC 2 cut(s) 363, 2167
BalI TGGCCA 1 cut(s) 1113
BamHI GGATCC 4 cut(s) 18, 296, 1300, 1854
BanII GRGCYC 1 cut(s) 2167
BbrPI CACGTG 1 cut(s) 3854
BbsI GAAGAC 5 cut(s) 678, 1269, 1805, 2556, 3576
Bbv12I GWGCWC 2 cut(s) 282, 1681
BbvI GCAGC 7 cut(s) 447, 1402, 1571, 2188, 2312, 3258, 3375
BceAI ACGGC 1 cut(s) 2569
BcgI CGANNNNNNTGC 2 cut(s) 953, 987
BciT130I CCWGG 1 cut(s) 3615
BciVI GTATCC 2 cut(s) 280, 1738
BcnI CCSGG 2 cut(s) 449, 3905
BcoDI GTCTC 7 cut(s) 728, 810, 2226, 2418, 3725, 3917, 4022
BfmI CTRYAG 2 cut(s) 238, 3625
BfrI CTTAAG 1 cut(s) 818
BfuI GTATCC 2 cut(s) 280, 1738
BisI GCNGC 8 cut(s) 436, 1391, 1560, 2202, 2326, 3247, 3250, 3389
BlnI CCTAGG 2 cut(s) 424, 998
BlsI GCNGC 8 cut(s) 437, 1392, 1561, 2203, 2327, 3248, 3251, 3390
BmcAI AGTACT 1 cut(s) 4052
Bme1390I CCNGG 3 cut(s) 449, 3615, 3905
Bme18I GGWCC 2 cut(s) 643, 3842
BmeT110I CYCGRG 1 cut(s) 2421
BmgBI CACGTC 1 cut(s) 2524
BmgT120I GGNCC 5 cut(s) 643, 2163, 2164, 2449, 3842
BmiI GGNNCC 6 cut(s) 20, 298, 1302, 1856, 2165, 3340
BmrFI CCNGG 3 cut(s) 449, 3615, 3905
BmrI ACTGGG 3 cut(s) 1299, 1860, 3088
BmuI ACTGGG 3 cut(s) 1299, 1860, 3088
BoxI GACNNNNGTC 1 cut(s) 3576
BpiI GAAGAC 5 cut(s) 678, 1269, 1805, 2556, 3576
BplI GAGNNNNNCTC 2 cut(s) 487, 519
BpmI CTGGAG 2 cut(s) 2675, 4231
BpuEI CTTGAG 2 cut(s) 2924, 3328
BpuMI CCSGG 2 cut(s) 449, 3905
BsaAI YACGTR 3 cut(s) 2108, 3416, 3854
BsaHI GRCGYC 2 cut(s) 737, 960
BsaI GGTCTC 1 cut(s) 4022
BsaJI CCNNGG 6 cut(s) 424, 528, 998, 1986, 3439, 3904
BsaWI WCCGGW 4 cut(s) 2665, 3287, 3346, 3864
BsaXI ACNNNNNCTCC 2 cut(s) 3252, 3282
Bsc4I CCNNNNNNNGG 7 cut(s) 306, 631, 879, 1987, 2973, 3665, 3982
Bse118I RCCGGY 1 cut(s) 2129
Bse1I ACTGG 9 cut(s) 300, 873, 957, 1305, 1818, 1855, 2600, 3083, 3356
BseAI TCCGGA 2 cut(s) 3346, 3864
BseBI CCWGG 1 cut(s) 3615
BseDI CCNNGG 6 cut(s) 424, 528, 998, 1986, 3439, 3904
BseLI CCNNNNNNNGG 7 cut(s) 306, 631, 879, 1987, 2973, 3665, 3982
BseMII CTCAG 4 cut(s) 3372, 3575, 3782, 3947
BseNI ACTGG 9 cut(s) 300, 873, 957, 1305, 1818, 1855, 2600, 3083, 3356
BseRI GAGGAG 2 cut(s) 1342, 1808
BseSI GKGCMC 2 cut(s) 363, 2167
BseXI GCAGC 7 cut(s) 447, 1402, 1571, 2188, 2312, 3258, 3375
BseYI CCCAGC 1 cut(s) 1777
BsgI GTGCAG 3 cut(s) 179, 447, 1409
Bsh1236I CGCG 2 cut(s) 1595, 2159
Bsh1285I CGRYCG 1 cut(s) 1075
BsiEI CGRYCG 1 cut(s) 1075
BsiHKAI GWGCWC 2 cut(s) 282, 1681
BsiHKCI CYCGRG 1 cut(s) 2421
BsiSI CCGG 8 cut(s) 449, 661, 2130, 2666, 3288, 3347, 3865, 3904
BslFI GGGAC 4 cut(s) 79, 123, 322, 2534
BslI CCNNNNNNNGG 7 cut(s) 306, 631, 879, 1987, 2973, 3665, 3982
BsmAI GTCTC 7 cut(s) 728, 810, 2226, 2418, 3725, 3917, 4022
BsmBI CGTCTC 2 cut(s) 728, 2226
BsmFI GGGAC 4 cut(s) 79, 123, 322, 2534
BsmI GAATGC 6 cut(s) 381, 2129, 3407, 3514, 3676, 4148
Bso31I GGTCTC 1 cut(s) 4022
BsoBI CYCGRG 1 cut(s) 2421
Bsp120I GGGCCC 1 cut(s) 2163
Bsp1286I GDGCHC 4 cut(s) 282, 363, 1681, 2167
Bsp13I TCCGGA 2 cut(s) 3346, 3864
Bsp1407I TGTACA 2 cut(s) 3054, 4080
Bsp19I CCATGG 1 cut(s) 1986
Bsp68I TCGCGA 1 cut(s) 2159
BspACI CCGC 6 cut(s) 321, 445, 2560, 3249, 3819, 4132
BspCNI CTCAG 4 cut(s) 3373, 3576, 3783, 3946
BspEI TCCGGA 2 cut(s) 3346, 3864
BspFNI CGCG 2 cut(s) 1595, 2159
BspHI TCATGA 1 cut(s) 1587
BspLI GGNNCC 6 cut(s) 20, 298, 1302, 1856, 2165, 3340
BspMAI CTGCAG 1 cut(s) 3629
BspQI GCTCTTC 2 cut(s) 546, 1877
BspTI CTTAAG 1 cut(s) 818
BspTNI GGTCTC 1 cut(s) 4022
BsrFI RCCGGY 1 cut(s) 2129
BsrGI TGTACA 2 cut(s) 3054, 4080
BsrI ACTGG 9 cut(s) 300, 873, 957, 1305, 1818, 1855, 2600, 3083, 3356
BssAI RCCGGY 1 cut(s) 2129
BssECI CCNNGG 6 cut(s) 424, 528, 998, 1986, 3439, 3904
BssNI GRCGYC 2 cut(s) 737, 960
BssT1I CCWWGG 3 cut(s) 424, 998, 1986
Bst2UI CCWGG 1 cut(s) 3615
BstACI GRCGYC 2 cut(s) 737, 960
BstAFI CTTAAG 1 cut(s) 818
BstAPI GCANNNNNTGC 1 cut(s) 695
BstAUI TGTACA 2 cut(s) 3054, 4080
BstBAI YACGTR 3 cut(s) 2108, 3416, 3854
BstC8I GCNNGC 6 cut(s) 381, 466, 687, 2468, 2558, 2660
BstDSI CCRYGG 2 cut(s) 1986, 3439
BstEII GGTNACC 1 cut(s) 254
BstFNI CGCG 2 cut(s) 1595, 2159
BstHHI GCGC 4 cut(s) 608, 1597, 2129, 4239
BstMAI GTCTC 7 cut(s) 728, 810, 2226, 2418, 3725, 3917, 4022
BstMCI CGRYCG 1 cut(s) 1075
BstMWI GCNNNNNNNGC 7 cut(s) 58, 385, 435, 444, 545, 695, 2150
BstNI CCWGG 1 cut(s) 3615
BstNSI RCATGY 4 cut(s) 2413, 3522, 3752, 3835
BstPAI GACNNNNGTC 1 cut(s) 3576
BstPI GGTNACC 1 cut(s) 254
BstSCI CCNGG 3 cut(s) 447, 3613, 3903
BstSFI CTRYAG 2 cut(s) 238, 3625
BstSLI GKGCMC 2 cut(s) 363, 2167
BstSNI TACGTA 1 cut(s) 3416
BstUI CGCG 2 cut(s) 1595, 2159
BstV1I GCAGC 7 cut(s) 447, 1402, 1571, 2188, 2312, 3258, 3375
BstV2I GAAGAC 5 cut(s) 678, 1269, 1805, 2556, 3576
BstX2I RGATCY 9 cut(s) 18, 296, 1300, 1854, 2730, 2776, 3302, 3617, 3811
BstYI RGATCY 9 cut(s) 18, 296, 1300, 1854, 2730, 2776, 3302, 3617, 3811
BsuI GTATCC 2 cut(s) 280, 1738
BtgI CCRYGG 2 cut(s) 1986, 3439
BtrI CACGTC 1 cut(s) 2524
BtsI GCAGTG 1 cut(s) 1400
BtsIMutI CAGTG 7 cut(s) 1312, 1400, 1442, 2995, 3488, 3674, 3941
BtuMI TCGCGA 1 cut(s) 2159
Cac8I GCNNGC 6 cut(s) 381, 466, 687, 2468, 2558, 2660
CciI TCATGA 1 cut(s) 1587
CfoI GCGC 4 cut(s) 608, 1597, 2129, 4239
Cfr10I RCCGGY 1 cut(s) 2129
Cfr13I GGNCC 5 cut(s) 643, 2163, 2164, 2449, 3842
CseI GACGC 2 cut(s) 745, 2208
Csp6I GTAC 6 cut(s) 1313, 2006, 2068, 3055, 4051, 4081
CspCI CAANNNNNGTGG 4 cut(s) 1603, 1638, 2127, 2162
CviQI GTAC 6 cut(s) 1313, 2006, 2068, 3055, 4051, 4081
DraI TTTAAA 4 cut(s) 40, 132, 1410, 1549
DraIII CACNNNGTG 1 cut(s) 1221
DrdI GACNNNNNNGTC 1 cut(s) 3356
DseDI GACNNNNNNGTC 1 cut(s) 3356
EaeI YGGCCR 4 cut(s) 1111, 2554, 2597, 3250
Eco105I TACGTA 1 cut(s) 3416
Eco130I CCWWGG 3 cut(s) 424, 998, 1986
Eco147I AGGCCT 1 cut(s) 429
Eco24I GRGCYC 1 cut(s) 2167
Eco31I GGTCTC 1 cut(s) 4022
Eco32I GATATC 3 cut(s) 2350, 3027, 3235
Eco47I GGWCC 2 cut(s) 643, 3842
Eco57I CTGAAG 2 cut(s) 2259, 2568
Eco72I CACGTG 1 cut(s) 3854
Eco88I CYCGRG 1 cut(s) 2421
Eco91I GGTNACC 1 cut(s) 254
EcoO109I RGGNCCY 4 cut(s) 643, 2163, 2164, 2449
EcoO65I GGTNACC 1 cut(s) 254
EcoRI GAATTC 2 cut(s) 1275, 1908
EcoRII CCWGG 1 cut(s) 3613
EcoRV GATATC 3 cut(s) 2350, 3027, 3235
EcoT14I CCWWGG 3 cut(s) 424, 998, 1986
EcoT22I ATGCAT 3 cut(s) 459, 2263, 2508
EcoT38I GRGCYC 1 cut(s) 2167
ErhI CCWWGG 3 cut(s) 424, 998, 1986
Esp3I CGTCTC 2 cut(s) 728, 2226
FalI AAGNNNNNCTT 2 cut(s) 389, 421
FaqI GGGAC 4 cut(s) 79, 123, 322, 2534
FauI CCCGC 1 cut(s) 3826
FauNDI CATATG 1 cut(s) 3531
FblI GTMKAC 2 cut(s) 1629, 2134
Fnu4HI GCNGC 8 cut(s) 436, 1391, 1560, 2202, 2326, 3247, 3250, 3389
FriOI GRGCYC 1 cut(s) 2167
Fsp4HI GCNGC 8 cut(s) 436, 1391, 1560, 2202, 2326, 3247, 3250, 3389
FspI TGCGCA 2 cut(s) 607, 4238
GlaI GCGC 4 cut(s) 607, 1596, 2128, 4238
GluI GCNGC 8 cut(s) 436, 1391, 1560, 2202, 2326, 3247, 3250, 3389
GsaI CCCAGC 1 cut(s) 1781
GsuI CTGGAG 2 cut(s) 2675, 4231
HapII CCGG 8 cut(s) 449, 661, 2130, 2666, 3288, 3347, 3865, 3904
HgaI GACGC 2 cut(s) 745, 2208
HhaI GCGC 4 cut(s) 608, 1597, 2129, 4239
Hin1I GRCGYC 2 cut(s) 737, 960
Hin6I GCGC 4 cut(s) 606, 1595, 2127, 4237
HinP1I GCGC 4 cut(s) 606, 1595, 2127, 4237
HincII GTYRAC 2 cut(s) 958, 2964
HindII GTYRAC 2 cut(s) 958, 2964
HindIII AAGCTT 5 cut(s) 381, 796, 1404, 2838, 2900
HpaII CCGG 8 cut(s) 449, 661, 2130, 2666, 3288, 3347, 3865, 3904
HphI GGTGA 8 cut(s) 209, 335, 865, 1046, 1675, 2455, 2581, 3614
Hpy166II GTNNAC 7 cut(s) 411, 958, 1250, 1630, 2135, 2964, 3226
Hpy8I GTNNAC 7 cut(s) 411, 958, 1250, 1630, 2135, 2964, 3226
Hpy99I CGWCG 1 cut(s) 965
HpyCH4IV ACGT 6 cut(s) 864, 960, 2107, 2523, 3415, 3853
HpyF10VI GCNNNNNNNGC 7 cut(s) 58, 385, 435, 444, 545, 695, 2150
HpySE526I ACGT 6 cut(s) 864, 960, 2107, 2523, 3415, 3853
Hsp92I GRCGYC 2 cut(s) 737, 960
HspAI GCGC 4 cut(s) 606, 1595, 2127, 4237
Kpn2I TCCGGA 2 cut(s) 3346, 3864
LguI GCTCTTC 2 cut(s) 546, 1877
LmnI GCTCC 6 cut(s) 118, 277, 1000, 1676, 2040, 3853
Lsp1109I GCAGC 7 cut(s) 447, 1402, 1571, 2188, 2312, 3258, 3375
MaeII ACGT 6 cut(s) 864, 960, 2107, 2523, 3415, 3853
MaeIII GTNAC 7 cut(s) 254, 323, 853, 3411, 3416, 3606, 3964
MfeI CAATTG 2 cut(s) 2355, 3947
MflI RGATCY 9 cut(s) 18, 296, 1300, 1854, 2730, 2776, 3302, 3617, 3811
MhlI GDGCHC 4 cut(s) 282, 363, 1681, 2167
MlsI TGGCCA 1 cut(s) 1113
MluNI TGGCCA 1 cut(s) 1113
MlyI GAGTC 6 cut(s) 521, 1635, 2761, 2778, 3276, 3862
MmeI TCCRAC 5 cut(s) 1627, 1654, 2430, 3024, 3789
Mox20I TGGCCA 1 cut(s) 1113
Mph1103I ATGCAT 3 cut(s) 459, 2263, 2508
MroI TCCGGA 2 cut(s) 3346, 3864
MroXI GAANNNNTTC 4 cut(s) 540, 839, 3881, 4068
MscI TGGCCA 1 cut(s) 1113
MslI CAYNNNNRTG 2 cut(s) 848, 3425
Msp20I TGGCCA 1 cut(s) 1113
MspA1I CMGCKG 2 cut(s) 2560, 3249
MspCI CTTAAG 1 cut(s) 818
MspI CCGG 8 cut(s) 449, 661, 2130, 2666, 3288, 3347, 3865, 3904
MspR9I CCNGG 3 cut(s) 449, 3615, 3905
MunI CAATTG 2 cut(s) 2355, 3947
Mva1269I GAATGC 6 cut(s) 381, 2129, 3407, 3514, 3676, 4148
MvaI CCWGG 1 cut(s) 3615
MvnI CGCG 2 cut(s) 1595, 2159
MwoI GCNNNNNNNGC 7 cut(s) 58, 385, 435, 444, 545, 695, 2150
NciI CCSGG 2 cut(s) 449, 3905
NcoI CCATGG 1 cut(s) 1986
NdeI CATATG 1 cut(s) 3531
NlaIV GGNNCC 6 cut(s) 20, 298, 1302, 1856, 2165, 3340
NmuCI GTSAC 2 cut(s) 323, 853
NruI TCGCGA 1 cut(s) 2159
NsbI TGCGCA 2 cut(s) 607, 4238
NsiI ATGCAT 3 cut(s) 459, 2263, 2508
NspI RCATGY 4 cut(s) 2413, 3522, 3752, 3835
PagI TCATGA 1 cut(s) 1587
PceI AGGCCT 1 cut(s) 429
PciI ACATGT 2 cut(s) 3518, 3748
PciSI GCTCTTC 2 cut(s) 546, 1877
PctI GAATGC 6 cut(s) 381, 2129, 3407, 3514, 3676, 4148
PdmI GAANNNNTTC 4 cut(s) 540, 839, 3881, 4068
PflMI CCANNNNNTGG 1 cut(s) 1987
PkrI GCNGC 8 cut(s) 437, 1392, 1561, 2203, 2327, 3248, 3251, 3390
Ple19I CGATCG 1 cut(s) 1075
PleI GAGTC 6 cut(s) 520, 1634, 2760, 2777, 3275, 3862
PmaCI CACGTG 1 cut(s) 3854
PmlI CACGTG 1 cut(s) 3854
PpsI GAGTC 6 cut(s) 520, 1634, 2760, 2777, 3275, 3862
Ppu21I YACGTR 3 cut(s) 2108, 3416, 3854
PpuMI RGGWCCY 1 cut(s) 643
PscI ACATGT 2 cut(s) 3518, 3748
PshAI GACNNNNGTC 1 cut(s) 3576
PsiI TTATAA 3 cut(s) 1293, 1452, 3396
Psp5II RGGWCCY 1 cut(s) 643
Psp6I CCWGG 1 cut(s) 3613
PspCI CACGTG 1 cut(s) 3854
PspEI GGTNACC 1 cut(s) 254
PspFI CCCAGC 1 cut(s) 1777
PspGI CCWGG 1 cut(s) 3613
PspN4I GGNNCC 6 cut(s) 20, 298, 1302, 1856, 2165, 3340
PspOMI GGGCCC 1 cut(s) 2163
PspPI GGNCC 5 cut(s) 643, 2163, 2164, 2449, 3842
PspPPI RGGWCCY 1 cut(s) 643
PstI CTGCAG 1 cut(s) 3629
PsuI RGATCY 9 cut(s) 18, 296, 1300, 1854, 2730, 2776, 3302, 3617, 3811
PvuI CGATCG 1 cut(s) 1075
RruI TCGCGA 1 cut(s) 2159
RsaI GTAC 6 cut(s) 1314, 2007, 2069, 3056, 4052, 4082
RsaNI GTAC 6 cut(s) 1313, 2006, 2068, 3055, 4051, 4081
RseI CAYNNNNRTG 2 cut(s) 848, 3425
SapI GCTCTTC 2 cut(s) 546, 1877
SatI GCNGC 8 cut(s) 436, 1391, 1560, 2202, 2326, 3247, 3250, 3389
Sau96I GGNCC 5 cut(s) 643, 2163, 2164, 2449, 3842
ScaI AGTACT 1 cut(s) 4052
SchI GAGTC 6 cut(s) 521, 1635, 2761, 2778, 3276, 3862
ScrFI CCNGG 3 cut(s) 449, 3615, 3905
SduI GDGCHC 4 cut(s) 282, 363, 1681, 2167
SfcI CTRYAG 2 cut(s) 238, 3625
SinI GGWCC 2 cut(s) 643, 3842
SmiMI CAYNNNNRTG 2 cut(s) 848, 3425
SmlI CTYRAG 3 cut(s) 818, 2903, 3307
SmoI CTYRAG 3 cut(s) 818, 2903, 3307
SnaBI TACGTA 1 cut(s) 3416
SseBI AGGCCT 1 cut(s) 429
SsiI CCGC 6 cut(s) 321, 445, 2560, 3249, 3819, 4132
SspI AATATT 1 cut(s) 3118
StuI AGGCCT 1 cut(s) 429
StyD4I CCNGG 3 cut(s) 447, 3613, 3903
StyI CCWWGG 3 cut(s) 424, 998, 1986
TaiI ACGT 6 cut(s) 867, 963, 2110, 2526, 3418, 3856
TaqI TCGA 8 cut(s) 963, 1075, 2170, 2206, 3210, 3231, 3283, 3996
TatI WGTACW 4 cut(s) 2005, 3054, 4050, 4080
TauI GCSGC 1 cut(s) 3252
TscAI CASTG 7 cut(s) 1312, 1400, 1449, 2995, 3488, 3681, 3941
TseFI GTSAC 2 cut(s) 323, 853
TseI GCWGC 7 cut(s) 435, 1390, 1559, 2201, 2325, 3246, 3388
Tsp45I GTSAC 2 cut(s) 323, 853
TspGWI ACGGA 2 cut(s) 1647, 2505
TspRI CASTG 7 cut(s) 1312, 1400, 1449, 2995, 3488, 3681, 3941
Van91I CCANNNNNTGG 1 cut(s) 1987
Vha464I CTTAAG 1 cut(s) 818
VpaK11BI GGWCC 2 cut(s) 643, 3842
XapI RAATTY 7 cut(s) 329, 1275, 1423, 1553, 1752, 1908, 2652
XceI RCATGY 4 cut(s) 2413, 3522, 3752, 3835
XcmI CCANNNNNNNNNTGG 3 cut(s) 819, 1153, 1222
XmaJI CCTAGG 2 cut(s) 424, 998
XmiI GTMKAC 2 cut(s) 1629, 2134
XmnI GAANNNNTTC 4 cut(s) 540, 839, 3881, 4068
ZraI GACGTC 1 cut(s) 961
ZrmI AGTACT 1 cut(s) 4052
Zsp2I ATGCAT 3 cut(s) 459, 2263, 2508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.