FvH4_3g44070

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000085
Physical Location & Seq
Reverse (-)
36771065 .. 36775697
4633 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44070.t1

Sequence Viewer

Length: 3987 bp
ATGGCTTCGACCTCCCCAGAAACTGCTGCATCAAATGAAGCATCATCATCAACAGCTCGTCGCTGGAAGGATGATGTGTTTTTGAGTTACAAGAGTGGTGACATTCCAAATGGTTTTATATTGCAATTATACGATAGGCTGCAACAGAGGAAGATCACAACGTTTATGGATCATTATTCAGATTTTCAAGTAGGGGATGCTATGTCTCCCTCTCTCCGAAAGGCAATTGAAGAATCAAAGTTGGCGGTTGTTGTTCTGTCCCAAAATTATGCTTCTTCTGCTTGGTGTTTGGAGGAACTTACAAAGTTGTGTCAATTCAGGGAGAACGGCTTTACAATTTTTCCAGTTTTTTACAAGGTTGAAGCCAGTAATGTAAGAAAGCAAAAGGGGAGTTTTCAGGATGCTTTCGCCAAGCATGAAAGGTCTGGGCGATACCCACCTCAGAAGGTTCAGCAGTGGAGAGATGCTTTAACAAAAGTGGGTTATTTGTCTGGGTTGAAAAAACGTTATTACAAGACTGACAATGATCTTGTCCAAGCCATTATGGAGTCTATGTGCAGTCAAATGAAGACTATTGAAATTATTTCGGAGTCAGATGAAGATCCCATTGTGGAATCAGAGTCAGATGAAGATTCCATTGTGGAACAGTGCACCAAGGATTTTGAAGCAGTTGAAGCAACATCAGAATCCTCTAATGGAAATTCTGAACCAGCTCCTCAGTGGAAGCATGATGTCTTTGTGAGCTTTCGGGGTAATGACACCCGCAAGGGTTTTCTATCCCATTTATACCATGAATTGCAAAACACGAAAGTAATAAGAGTATTTAAGGATGATGAACAACTTAAGAAAGGGAAGGATATTTCTCAAAGTCTGCTGACGGCAATTGAAGAATCAAGGTTTGCGATCATTGTTCTGTCGGAGAACTATGCTTCTTCTGCATGGTGTTTAGATGAAATTACAAAGATTTTTCAATGCATGGAAGGCAAGGATACAATTCTGCCAATTTTTTATCATGTGGATCCCTCTGATGTACGATATCAAAAGAAAAGTTATGAAGCAGCCTTTAAAATGCATTCAAGAATTACAAGGTATGACATAAAGAAGGTGAAGCAGTGGAGAGCTGATTTAACAAAAGTGGGGAATCTCTCTGGGTGGGATACAAAGAACTACAGATCTGAAAGGGAACTTGTTGAAGACATTGTGAAAGTTGTTCTGAAAGAAGTACTTCAAACACCAAGGGTAGATTTTGAAGAATTTGAAGCTACAAGAGAAGCAATGGATGAGGTTAAGAGGATGTTGAAAGATGATAATATCACCGTCATTGGGGTCTATGGAAGAGGAGGTGTTGGCAAGACAGCCATGGTGCAAAATGTCAGTGCACAAGCCAAAAGAATTGGGCTTATCGATCATGTTGTTATGGCTGTTATATCCCGAAACCCTGATTTGCAAAAAATTCAAGGTGAATTGGTGAATCAGCTCGGATTGGAATTGGTGGAGGATGGAGAAGTGGAAAGAGCCAGTAGATTGAGGGAGAGGATTATGGGAGGAAAGAAGATCCTTATTATCTTGGACGACATTTGGGAGATAATAGATTTGTCAAGCATTGGAATTCCTAGCTATGACGAGCTTCAAAGATGCAATTCCAAGGTCCTACTCACCACAAGGATCAGGAGTCTTTGTCATTCTATGGGGTGCCAAGAACCTATTCCTCTCAAGATCCTATCAGAAGATGATTCTTTGAAATTCTTCATGAAGAAGTCGAGAAAGCCTTTTCATGGATCTGCCGATTCCGATGATGAAGAGAAGAAAATAGTTAGAGAATGTGATGGTCTGCCAAGTTTATTGGAAGAAGCTGCAAGGGAATTTGGAGATAAATACGTGAAAGAATGGAATGAAGCAGTTTCAAAGGAATTTGGAGATAATGAGGATGAATTGCAAAAATCAGCTCGAGAAGTAAATGCATCTAAACTTGTCCCCACTAAAGATGAGAGAGATATATTTAGAAGGATAGAGTTATGCTATCATACCTCGAGGTTTGATGATGCCAAGTCCTTTTTCTTGCTTTGCTGCATGTTTCCAGAAGATTATGATATTCCATTTGAAAAATTGTTGACCTACGGCATTGGAAAAGGTTTGCTTCTATATTCTGACACGTTGCAAGAAGCTAGAGCCAGAGCACATTCTTTGGTCGCATACCTTCAAGCTTCAGGGTTGCTTTTGGATGGTAAGAAGGGATGCATAAAAATGCATTGTGACATCCGAAATGCGGCCATGTCTATTTTGTTGTCTGAAGAAGGTCATGCCTTTTTCGTAAAAGCTGGTTGTGATTTGAAGTATTGGCCAATGGATGCCCATGAAGGCTACTTGGCAATATCACTAATGGGGAACAATCTTAACAAGCTTCCCGAAACTTTGGTATGTTCTGAACTCCAAATTTTACTGCTACAGAATAATGCTGGTATATCTGACATTCCAGAAAACTTTTTTCAAAGTCCAAATGCACTAAGGGTCTTTGATATTAGCTATACCGGTATTTCAGTGCTACCCTCTTCATTCAATCTCCTCACCAACCTCCAAGCCTTGCATTTGGATTGTTGTAATAGAATAACTGACATATCCGTACTTGGGAAGCTGAAGAAAGTAGAGATTCTTAGTTTGAGAAAATTTCCTCATAAGGATTTCCCAAAAGAGATAGGAAATTTGACCAGTCTAAGGATGCTGGACATCACTGGTGGATGTGTTCGAAAAATTCCATCCAAAGTGATATCGAGGTTGTATAGACTGGAAGAATTGTACATGAAATGTGAATTTGGGGAATGGGGAAGTGAAGTCGAGGAAGCAGTAGGAGAAACAAATGCTGACTTTGATGAGATAGTTGGCTTGTCCCGTTTAAACATTTTGAAGGTTTACATATCTGATGCAGCTTGCTTACCCAAAGATATTGGGTACTTTGAGCTAAATTGGGTAAACTTTGACATATGCATCAGCAGAGATCTCTCTTCGGAGATGATGCTTGCTCCGAATGATGAAGCTTCAAGAACCTTGACTCTTGACAGAACAATCAATGACTTACCGGATTGGTTTATCAATGTGGTGACAGAGAAAGCAGAAAAACTGGTGTACAGAGATTGTAAGGGAGTAAACAACATTGTTAAGGAATATGAGCATGGGAGATTACATGGGCTGAAGCATGTTGCTGTCATTGGGTGCCTTGAGAACTTGAAAGAGTTGATAAATGCAACAACATGGGATCCAAGTAAACCTTTGTTCGAGAGCTTGGAAGAGTTGCATCTCCTTCACTTGCAACACCTCAAGGAGTTGTGTGTTGGCGATTTACCACTTGGGTCTCTTCGCAATCTCAAGTTGTTGAAAGTGCAAACTTGCTGCAACTTGGTGAATGCACTTTTACCGCAAAAGTTGTTGCAGAGACTAACAAATCTAGAAACAATATTTTGTGTAAACATGGAACACATGAAATATGTGTTTGGATTTGAACGGCTCGAGCTTGGAACAATTCTCTTGCCAAAATTGAGCGAGATGAGATTGTGCGCTCTCTTCGAACTAATAAGCATCTGGGATGGTCCTGCTCCACATGCAGTCTTTCATAATCTCAAAGTTTTGGCAGTTTGCAAGTGTACAAAACTGAAAACTCTATTCCCATTTGATGTAGCTCGATCTTTTGTGCAATTGGAAGACCTCTCTGTGGAGCGCTGCCCTAACTTGGGTAGAGTTATTGAAGCATGCAACAGCTTTGACAGATATATCATTTTTCCACAATTGAAGAAATTAGCTTTGATAAATCTTTCAGAGCTAACATGGTTCTGCACTGAAAATGGTTCTATTGGTATTGAGTGCCCTCTATTGGAATACATGTATGTGGATAAGTGCCCCCACCTCATAAACACTGCTTCTACTTCTGTTAAACTCAATGATCAACAACATTTGAATTCTCTAGCAAAAAGATGGGAGGATATGTATAAGCTATAA

Protein Analysis

1329

Amino Acids

151.39

Weight (kDa)

5.64

Isoelectric Point (pI)

42.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 25 - 192 1.5e-37 TIR domain
TIR_2 PF13676 26 - 121 4.9e-15 TIR domain
TIR PF01582 242 - 412 5e-49 TIR domain
TIR_2 PF13676 245 - 341 4.2e-16 TIR domain
NB-ARC PF00931 425 - 587 6.1e-28 NB-ARC domain
LRR_8 PF13855 809 - 865 8.2e-07 Leucine rich repeat
LRR_14 PF23598 810 - 934 3e-12 Leucine-rich repeat region
LRR_RPS2 PF23247 1093 - 1238 3.6e-23 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 2029
AccB1I GGYRCC 2 cut(s) 1692, 3207
AciI CCGC 4 cut(s) 245, 763, 2267, 3410
AclI AACGTT 2 cut(s) 161, 505
AcoI YGGCCR 2 cut(s) 2268, 2339
AcuI CTGAAG 4 cut(s) 2190, 2310, 2654, 3206
AfaI GTAC 7 cut(s) 1032, 1224, 2622, 2795, 2948, 3122, 3637
AfeI AGCGCT 1 cut(s) 3710
AfiI CCNNNNNNNGG 9 cut(s) 1323, 1775, 2266, 2625, 2712, 3703, 3721, 3722, 3862
AflII CTTAAG 1 cut(s) 842
AflIII ACRYGT 2 cut(s) 2151, 3870
AgeI ACCGGT 1 cut(s) 2528
AjuI GAANNNNNNNTTGG 4 cut(s) 1689, 1721, 2039, 2071
Alw21I GWGCWC 3 cut(s) 653, 1381, 2179
Alw26I GTCTC 3 cut(s) 210, 3351, 3421
Alw44I GTGCAC 2 cut(s) 649, 1377
AlwNI CAGNNNCTG 1 cut(s) 23
Ama87I CYCGRG 3 cut(s) 1947, 2029, 3500
Aor51HI AGCGCT 1 cut(s) 3710
AoxI GGCC 2 cut(s) 2268, 2339
ApaLI GTGCAC 2 cut(s) 649, 1377
ApeKI GCWGC 8 cut(s) 26, 139, 1058, 1853, 2067, 2921, 3384, 3711
AsiGI ACCGGT 1 cut(s) 2528
Asp700I GAANNNNTTC 3 cut(s) 1224, 1704, 1745
AspLEI GCGC 2 cut(s) 3551, 3711
AspS9I GGNCC 2 cut(s) 1648, 3581
AsuHPI GGTGA 9 cut(s) 110, 1117, 1306, 1472, 1480, 1648, 2557, 3106, 3406
AsuII TTCGAA 2 cut(s) 2743, 3558
AvaI CYCGRG 3 cut(s) 1947, 2029, 3500
AvaII GGWCC 2 cut(s) 1648, 3581
BaeGI GKGCMC 4 cut(s) 653, 1381, 3857, 3890
BalI TGGCCA 1 cut(s) 2341
BamHI GGATCC 2 cut(s) 1018, 3250
BanI GGYRCC 2 cut(s) 1692, 3207
BbsI GAAGAC 3 cut(s) 575, 1200, 3699
Bbv12I GWGCWC 3 cut(s) 653, 1381, 2179
BbvI GCAGC 8 cut(s) 13, 126, 1070, 1840, 2054, 2933, 3371, 3698
BccI CCATC 6 cut(s) 1493, 1820, 2216, 2761, 3572, 3957
BceAI ACGGC 4 cut(s) 343, 894, 2134, 3512
BciVI GTATCC 2 cut(s) 982, 1150
BclI TGATCA 1 cut(s) 3931
BcoDI GTCTC 3 cut(s) 210, 3351, 3421
BfaI CTAG 4 cut(s) 1614, 2166, 3440, 3953
BfmI CTRYAG 2 cut(s) 1168, 2444
BfoI RGCGCY 1 cut(s) 3712
BfrI CTTAAG 1 cut(s) 842
BfuI GTATCC 2 cut(s) 982, 1150
BglII AGATCT 2 cut(s) 1172, 2992
BisI GCNGC 9 cut(s) 27, 140, 1059, 1854, 2068, 2268, 2922, 3385, 3712
BlsI GCNGC 9 cut(s) 28, 141, 1060, 1855, 2069, 2269, 2923, 3386, 3713
BmcAI AGTACT 1 cut(s) 1224
Bme18I GGWCC 2 cut(s) 1648, 3581
BmeT110I CYCGRG 3 cut(s) 1947, 2029, 3500
BmgT120I GGNCC 2 cut(s) 1648, 3581
BmiI GGNNCC 4 cut(s) 1020, 1694, 3209, 3252
BpiI GAAGAC 3 cut(s) 575, 1200, 3699
Bpu14I TTCGAA 2 cut(s) 2743, 3558
BpuEI CTTGAG 4 cut(s) 1697, 3233, 3296, 3344
Bsa29I ATCGAT 1 cut(s) 1404
BsaAI YACGTR 1 cut(s) 1879
BsaBI GATNNNNATC 1 cut(s) 600
BsaI GGTCTC 1 cut(s) 3351
BsaJI CCNNGG 4 cut(s) 654, 1235, 1359, 1644
BsaWI WCCGGW 2 cut(s) 2528, 3073
BsaXI ACNNNNNCTCC 5 cut(s) 539, 569, 1663, 1693, 3959
Bsc4I CCNNNNNNNGG 9 cut(s) 1323, 1775, 2266, 2625, 2712, 3703, 3721, 3722, 3862
Bse118I RCCGGY 1 cut(s) 2528
Bse1I ACTGG 7 cut(s) 344, 366, 1518, 2706, 2734, 2787, 3120
Bse3DI GCAATG 1 cut(s) 1281
Bse8I GATNNNNATC 1 cut(s) 600
BseCI ATCGAT 1 cut(s) 1404
BseDI CCNNGG 4 cut(s) 654, 1235, 1359, 1644
BseJI GATNNNNATC 1 cut(s) 600
BseLI CCNNNNNNNGG 9 cut(s) 1323, 1775, 2266, 2625, 2712, 3703, 3721, 3722, 3862
BseMI GCAATG 1 cut(s) 1281
BseMII CTCAG 2 cut(s) 455, 731
BseNI ACTGG 7 cut(s) 344, 366, 1518, 2706, 2734, 2787, 3120
BseRI GAGGAG 3 cut(s) 705, 1353, 2552
BseSI GKGCMC 4 cut(s) 653, 1381, 3857, 3890
BseXI GCAGC 8 cut(s) 13, 126, 1070, 1840, 2054, 2933, 3371, 3698
BsgI GTGCAG 2 cut(s) 577, 3808
BshFI GGCC 2 cut(s) 2270, 2341
BshNI GGYRCC 2 cut(s) 1692, 3207
BshTI ACCGGT 1 cut(s) 2528
BshVI ATCGAT 1 cut(s) 1404
BsiHKAI GWGCWC 3 cut(s) 653, 1381, 2179
BsiHKCI CYCGRG 3 cut(s) 1947, 2029, 3500
BsiSI CCGG 2 cut(s) 2529, 3074
BslFI GGGAC 3 cut(s) 244, 1958, 2869
BslI CCNNNNNNNGG 9 cut(s) 1323, 1775, 2266, 2625, 2712, 3703, 3721, 3722, 3862
BsmAI GTCTC 3 cut(s) 210, 3351, 3421
BsmFI GGGAC 3 cut(s) 244, 1958, 2869
BsmI GAATGC 2 cut(s) 1072, 3403
BsnI GGCC 2 cut(s) 2270, 2341
Bso31I GGTCTC 1 cut(s) 3351
BsoBI CYCGRG 3 cut(s) 1947, 2029, 3500
Bsp119I TTCGAA 2 cut(s) 2743, 3558
Bsp1286I GDGCHC 5 cut(s) 653, 1381, 2179, 3857, 3890
Bsp1407I TGTACA 3 cut(s) 2793, 3120, 3635
Bsp19I CCATGG 1 cut(s) 1359
BspACI CCGC 4 cut(s) 245, 763, 2267, 3410
BspANI GGCC 2 cut(s) 2270, 2341
BspCNI CTCAG 2 cut(s) 454, 730
BspDI ATCGAT 1 cut(s) 1404
BspHI TCATGA 1 cut(s) 1749
BspLI GGNNCC 4 cut(s) 1020, 1694, 3209, 3252
BspT104I TTCGAA 2 cut(s) 2743, 3558
BspT107I GGYRCC 2 cut(s) 1692, 3207
BspTI CTTAAG 1 cut(s) 842
BspTNI GGTCTC 1 cut(s) 3351
BsrDI GCAATG 1 cut(s) 1281
BsrFI RCCGGY 1 cut(s) 2528
BsrGI TGTACA 3 cut(s) 2793, 3120, 3635
BsrI ACTGG 7 cut(s) 344, 366, 1518, 2706, 2734, 2787, 3120
BssAI RCCGGY 1 cut(s) 2528
BssECI CCNNGG 4 cut(s) 654, 1235, 1359, 1644
BssT1I CCWWGG 4 cut(s) 654, 1235, 1359, 1644
Bst4CI ACNGT 2 cut(s) 648, 1318
Bst6I CTCTTC 7 cut(s) 1330, 1794, 2554, 3004, 3276, 3354, 3560
BstAFI CTTAAG 1 cut(s) 842
BstAUI TGTACA 3 cut(s) 2793, 3120, 3635
BstBAI YACGTR 1 cut(s) 1879
BstBI TTCGAA 2 cut(s) 2743, 3558
BstC8I GCNNGC 3 cut(s) 2926, 3015, 3742
BstDEI CTNAG 5 cut(s) 441, 717, 2504, 2651, 2711
BstDSI CCRYGG 1 cut(s) 1359
BstH2I RGCGCY 1 cut(s) 3712
BstHHI GCGC 2 cut(s) 3551, 3711
BstMAI GTCTC 3 cut(s) 210, 3351, 3421
BstMWI GCNNNNNNNGC 5 cut(s) 278, 674, 935, 981, 3593
BstNSI RCATGY 5 cut(s) 2074, 3194, 3596, 3744, 3874
BstSFI CTRYAG 2 cut(s) 1168, 2444
BstSLI GKGCMC 4 cut(s) 653, 1381, 3857, 3890
BstV1I GCAGC 8 cut(s) 13, 126, 1070, 1840, 2054, 2933, 3371, 3698
BstV2I GAAGAC 3 cut(s) 575, 1200, 3699
BstX2I RGATCY 8 cut(s) 601, 1018, 1172, 1554, 1716, 1778, 2992, 3250
BstYI RGATCY 8 cut(s) 601, 1018, 1172, 1554, 1716, 1778, 2992, 3250
Bsu15I ATCGAT 1 cut(s) 1404
BsuI GTATCC 2 cut(s) 982, 1150
BsuRI GGCC 2 cut(s) 2270, 2341
BsuTUI ATCGAT 1 cut(s) 1404
BtgI CCRYGG 1 cut(s) 1359
BtsI GCAGTG 3 cut(s) 461, 1118, 3903
BtsIMutI CAGTG 9 cut(s) 461, 653, 725, 1118, 1381, 2544, 2727, 3825, 3903
Cac8I GCNNGC 3 cut(s) 2926, 3015, 3742
CaiI CAGNNNCTG 1 cut(s) 23
CciI TCATGA 1 cut(s) 1749
CfoI GCGC 2 cut(s) 3551, 3711
Cfr10I RCCGGY 1 cut(s) 2528
Cfr13I GGNCC 2 cut(s) 1648, 3581
ClaI ATCGAT 1 cut(s) 1404
Csp6I GTAC 7 cut(s) 1031, 1223, 2621, 2794, 2947, 3121, 3636
CspAI ACCGGT 1 cut(s) 2528
CviQI GTAC 7 cut(s) 1031, 1223, 2621, 2794, 2947, 3121, 3636
DdeI CTNAG 5 cut(s) 441, 717, 2504, 2651, 2711
DraI TTTAAA 2 cut(s) 1066, 2892
EaeI YGGCCR 2 cut(s) 2268, 2339
Eam1104I CTCTTC 7 cut(s) 1330, 1794, 2554, 3004, 3276, 3354, 3560
EarI CTCTTC 7 cut(s) 1330, 1794, 2554, 3004, 3276, 3354, 3560
Eco130I CCWWGG 4 cut(s) 654, 1235, 1359, 1644
Eco31I GGTCTC 1 cut(s) 3351
Eco32I GATATC 2 cut(s) 1037, 2766
Eco47I GGWCC 2 cut(s) 1648, 3581
Eco47III AGCGCT 1 cut(s) 3710
Eco57I CTGAAG 4 cut(s) 2190, 2310, 2654, 3206
Eco88I CYCGRG 3 cut(s) 1947, 2029, 3500
EcoO109I RGGNCCY 1 cut(s) 1648
EcoRI GAATTC 2 cut(s) 1608, 3946
EcoRV GATATC 2 cut(s) 1037, 2766
EcoT14I CCWWGG 4 cut(s) 654, 1235, 1359, 1644
EcoT22I ATGCAT 6 cut(s) 977, 1074, 1963, 2240, 2250, 2984
ErhI CCWWGG 4 cut(s) 654, 1235, 1359, 1644
FalI AAGNNNNNCTT 6 cut(s) 1209, 1241, 2121, 2153, 3247, 3279
FaqI GGGAC 3 cut(s) 244, 1958, 2869
FauI CCCGC 1 cut(s) 770
FauNDI CATATG 1 cut(s) 2978
FbaI TGATCA 1 cut(s) 3931
Fnu4HI GCNGC 9 cut(s) 27, 140, 1059, 1854, 2068, 2268, 2922, 3385, 3712
Fsp4HI GCNGC 9 cut(s) 27, 140, 1059, 1854, 2068, 2268, 2922, 3385, 3712
FspBI CTAG 4 cut(s) 1614, 2166, 3440, 3953
GlaI GCGC 2 cut(s) 3550, 3710
GluI GCNGC 9 cut(s) 27, 140, 1059, 1854, 2068, 2268, 2922, 3385, 3712
HaeII RGCGCY 1 cut(s) 3712
HaeIII GGCC 2 cut(s) 2270, 2341
HapII CCGG 2 cut(s) 2529, 3074
HhaI GCGC 2 cut(s) 3551, 3711
Hin6I GCGC 2 cut(s) 3549, 3709
HinP1I GCGC 2 cut(s) 3549, 3709
HincII GTYRAC 1 cut(s) 2112
HindII GTYRAC 1 cut(s) 2112
HindIII AAGCTT 3 cut(s) 2202, 2399, 3030
HpaII CCGG 2 cut(s) 2529, 3074
HphI GGTGA 9 cut(s) 110, 1117, 1306, 1472, 1480, 1648, 2557, 3106, 3406
Hpy99I CGWCG 1 cut(s) 63
HpyCH4III ACNGT 2 cut(s) 648, 1318
HpyCH4IV ACGT 4 cut(s) 161, 505, 1878, 2153
HpyF10VI GCNNNNNNNGC 5 cut(s) 278, 674, 935, 981, 3593
HpyF3I CTNAG 5 cut(s) 441, 717, 2504, 2651, 2711
HpySE526I ACGT 4 cut(s) 161, 505, 1878, 2153
HspAI GCGC 2 cut(s) 3549, 3709
Ksp22I TGATCA 1 cut(s) 3931
LmnI GCTCC 4 cut(s) 718, 3022, 3592, 3706
Lsp1109I GCAGC 8 cut(s) 13, 126, 1070, 1840, 2054, 2933, 3371, 3698
MaeI CTAG 4 cut(s) 1614, 2166, 3440, 3953
MaeII ACGT 4 cut(s) 161, 505, 1878, 2153
MaeIII GTNAC 4 cut(s) 86, 98, 2252, 3094
MfeI CAATTG 4 cut(s) 225, 882, 3686, 3776
MflI RGATCY 8 cut(s) 601, 1018, 1172, 1554, 1716, 1778, 2992, 3250
MhlI GDGCHC 5 cut(s) 653, 1381, 2179, 3857, 3890
MlsI TGGCCA 1 cut(s) 2341
MluNI TGGCCA 1 cut(s) 2341
MlyI GAGTC 5 cut(s) 557, 599, 629, 1681, 3040
MmeI TCCRAC 1 cut(s) 897
Mox20I TGGCCA 1 cut(s) 2341
Mph1103I ATGCAT 6 cut(s) 977, 1074, 1963, 2240, 2250, 2984
MroXI GAANNNNTTC 3 cut(s) 1224, 1704, 1745
MscI TGGCCA 1 cut(s) 2341
MslI CAYNNNNRTG 2 cut(s) 2243, 3875
Msp20I TGGCCA 1 cut(s) 2341
MspCI CTTAAG 1 cut(s) 842
MspI CCGG 2 cut(s) 2529, 3074
MssI GTTTAAAC 1 cut(s) 2892
MunI CAATTG 4 cut(s) 225, 882, 3686, 3776
Mva1269I GAATGC 2 cut(s) 1072, 3403
MwoI GCNNNNNNNGC 5 cut(s) 278, 674, 935, 981, 3593
NcoI CCATGG 1 cut(s) 1359
NdeI CATATG 1 cut(s) 2978
NlaIV GGNNCC 4 cut(s) 1020, 1694, 3209, 3252
NmuCI GTSAC 3 cut(s) 98, 2252, 3094
NsiI ATGCAT 6 cut(s) 977, 1074, 1963, 2240, 2250, 2984
NspI RCATGY 5 cut(s) 2074, 3194, 3596, 3744, 3874
NspV TTCGAA 2 cut(s) 2743, 3558
PaeI GCATGC 1 cut(s) 3744
PaeR7I CTCGAG 3 cut(s) 1947, 2029, 3500
PagI TCATGA 1 cut(s) 1749
PciI ACATGT 1 cut(s) 3870
PctI GAATGC 2 cut(s) 1072, 3403
PdmI GAANNNNTTC 3 cut(s) 1224, 1704, 1745
PinAI ACCGGT 1 cut(s) 2528
PkrI GCNGC 9 cut(s) 28, 141, 1060, 1855, 2069, 2269, 2923, 3386, 3713
PleI GAGTC 5 cut(s) 556, 598, 628, 1680, 3040
PmeI GTTTAAAC 1 cut(s) 2892
PpsI GAGTC 5 cut(s) 556, 598, 628, 1680, 3040
Ppu21I YACGTR 1 cut(s) 1879
PpuMI RGGWCCY 1 cut(s) 1648
PscI ACATGT 1 cut(s) 3870
Psp1406I AACGTT 2 cut(s) 161, 505
Psp5II RGGWCCY 1 cut(s) 1648
PspN4I GGNNCC 4 cut(s) 1020, 1694, 3209, 3252
PspPI GGNCC 2 cut(s) 1648, 3581
PspPPI RGGWCCY 1 cut(s) 1648
PspXI VCTCGAGB 2 cut(s) 2029, 3500
PstNI CAGNNNCTG 1 cut(s) 23
PsuI RGATCY 8 cut(s) 601, 1018, 1172, 1554, 1716, 1778, 2992, 3250
RsaI GTAC 7 cut(s) 1032, 1224, 2622, 2795, 2948, 3122, 3637
RsaNI GTAC 7 cut(s) 1031, 1223, 2621, 2794, 2947, 3121, 3636
RseI CAYNNNNRTG 2 cut(s) 2243, 3875
SatI GCNGC 9 cut(s) 27, 140, 1059, 1854, 2068, 2268, 2922, 3385, 3712
Sau96I GGNCC 2 cut(s) 1648, 3581
ScaI AGTACT 1 cut(s) 1224
SchI GAGTC 5 cut(s) 557, 599, 629, 1681, 3040
SduI GDGCHC 5 cut(s) 653, 1381, 2179, 3857, 3890
SfcI CTRYAG 2 cut(s) 1168, 2444
Sfr274I CTCGAG 3 cut(s) 1947, 2029, 3500
SfuI TTCGAA 2 cut(s) 2743, 3558
SinI GGWCC 2 cut(s) 1648, 3581
SlaI CTCGAG 3 cut(s) 1947, 2029, 3500
SmiMI CAYNNNNRTG 2 cut(s) 2243, 3875
SmlI CTYRAG 8 cut(s) 842, 1712, 1947, 2029, 3212, 3311, 3359, 3500
SmoI CTYRAG 8 cut(s) 842, 1712, 1947, 2029, 3212, 3311, 3359, 3500
SphI GCATGC 1 cut(s) 3744
SsiI CCGC 4 cut(s) 245, 763, 2267, 3410
SspI AATATT 1 cut(s) 3450
SspMI CTAG 4 cut(s) 1614, 2166, 3440, 3953
StyI CCWWGG 4 cut(s) 654, 1235, 1359, 1644
TaaI ACNGT 2 cut(s) 648, 1318
TaiI ACGT 4 cut(s) 164, 508, 1881, 2156
TatI WGTACW 4 cut(s) 1222, 2793, 3120, 3635
TauI GCSGC 1 cut(s) 2270
TscAI CASTG 9 cut(s) 461, 653, 725, 1118, 1381, 2544, 2734, 3832, 3910
TseFI GTSAC 3 cut(s) 98, 2252, 3094
TseI GCWGC 8 cut(s) 26, 139, 1058, 1853, 2067, 2921, 3384, 3711
Tsp45I GTSAC 3 cut(s) 98, 2252, 3094
TspGWI ACGGA 1 cut(s) 2608
TspRI CASTG 9 cut(s) 461, 653, 725, 1118, 1381, 2544, 2734, 3832, 3910
Vha464I CTTAAG 1 cut(s) 842
VneI GTGCAC 2 cut(s) 649, 1377
VpaK11BI GGWCC 2 cut(s) 1648, 3581
XbaI TCTAGA 1 cut(s) 3439
XceI RCATGY 5 cut(s) 2074, 3194, 3596, 3744, 3874
XcmI CCANNNNNNNNNTGG 1 cut(s) 717
XhoI CTCGAG 3 cut(s) 1947, 2029, 3500
XmnI GAANNNNTTC 3 cut(s) 1224, 1704, 1745
XspI CTAG 4 cut(s) 1614, 2166, 3440, 3953
ZrmI AGTACT 1 cut(s) 1224
Zsp2I ATGCAT 6 cut(s) 977, 1074, 1963, 2240, 2250, 2984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.