Rh7DG275900

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000085
Physical Location & Seq
Forward (+)
29696549 .. 29700746
4198 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG275900.1

Sequence Viewer

Length: 2934 bp
ATGAGGAGCCAAGAAAATATCACCCTCAGTACCCTATCGAAAGAAGATTCATGGACATTGTTTGTGAAATATGCAAGAAAGTCTTTTGAGTCGACCTATTTCGAGGATGTTGCGAGGAAGGTAGCTAGAGAATGTGGTGGTCTACCAATTGCATTGATAGCAGTTGCAAGGGCACTTGGAGATAAAGACCTGACAGAATGGGAATACGCAGCTCAACGGCTAGAGAAGTCGCGTGCTGCTAACCTTGACGATAATGGAGAAGCATCCGAATGCATAAAACTAAGTTATGATTTCTTGAAAGATGAGGACTCCAAGTCATGCTTCTTGCTTTGTTGCCTATTCCCAGAAGACTATGACATCCGAATAGAAGACTTGTTCAAGTATGGGATCGGGATCGGATTGTTTCAAGATACTGACACAATCCAAGAAGCCAGAGGAAAAGTTGGTTCAGTGGCCAAGCACCTTACACATTCTAGCTTGCTTTTGGATAGGGGAATTGATGGATGTGTTAGGATGCATGATGTCATCCGGGATACAGCCATTCAAATTGCACGATCTGAAGACGAGCACGGATTTTTCGTAGAAGCTGGCTGTGGTTTGAAGAATTGGCCACGTCGATTGCGTGAAGGCCATTCTGCAATTTCAGTCATGACGAACGACATTCGCGAGCTACCTGAAGAGCTGGTATGTCCGAAGCTCCAAATTTTATTGCTACAAGAGAATTATTTTTTAGATCAGATCCCTGATACTTGTTTCAAAAATACTAGTTTACTAAGAGTCGTCGATCTTAGTAACACTAGTATATCATCCCTATCCATGTCATTTATTCACCTAAGCAATCTCCAAGGTTTGTATTTAGATTCTTGCCAGAATATATCTGACATTTCCATACTGAAAAATTTTAAGAAGCTTGAGATTCTCAGTATGAAAAAGTACCCTCTAAAGGAATTGTCAACAGAAATAAGAAATTTGACCAATTTAAGGGTGTTGGATGTTAGTAGCCAGGGTTCAGTGCGTGGAGGTATCGTCAGAATTCCATCTGGGGTGATATCAGAATTGCGTAAATTAGAAGAACTGTGCTTGCAATGTGGATTTTGGGACTGGAGGAGTCAAGTTGAGGGAGAACGAGAAACAACTAATGTTAGCTTTGATGAGGTAACTAGCTTATCATATTTGAACAGTTTGAAGGTTTGCATATCTGACGCGAATTGCATCCCTAGAAGGGTTTGGGTCAATCTGAGTTGGAATGACTTTGATATATGTATTGGTAGAGACAGGAACACCAGAAATATTATCAGAAACCAGCATAGTCAACTCTGGCGAGATGGTCATAAATATTCAAGATCTTTGGTTCTTGATACAACCATTTGTATGTTACCAGCTTGGTTTGTCAGCGTGGTGACAGAGAATACAGAGAGGCTAATGTATGTAAATTTCACAGGGTTAAATAACATTCTCGTGGAATATCACCATGGGATGTTACATAGTCTGAAGCATCTCTCAGTTATTGGTCCCAATGAGAGCTTGGTAACATTGATCAATTTGGAGGCAATACCAAGTATGCGAAATAAACCTGTGTTCGAGAAATTGGAAGAATTGCATCTGGAGAGTATGGGTTCCCTGAAGGAGTTATGTTCTAGTGAATTACCATCTAGGTCTCTCTGGAATCTGAAGGTGTTGCAGGTACATGATTGTCCTAAGTTGGGAAATGTCCTGTTACCATCAAAATTGTTACAGAGACTGGCAAATCTGGAGAACCTATTCTGTAAGTGTGTGCCTGAAATGGAATATGTGTTTGGATGTGAAGGATTTGAGCTAGAACAATCAAAACTGAGAAAGATGAACTTGTTTGAGCTAGAAGCAGTAAAAAGCATATGTAATGGTCCTGCTCCACCTGCAATGTTCCAAGCGCTTCACAGTTTGTCAATTTACCGTTGCAAATTTCACGGATGTCTCTTCACATTTGATGTAGCTCAATGTCTTTTTCAAGTGGAAATCATTTCAGTACAGAGTTGCCCTATCTTGGAAAGAGTAATCGAAGCAAGCAAAGAATCAGAGCTCAGTAAGAAGACCGTTCTTCCAAAATTGAAGTACCTAGGCTTGGTGGATCTTCCTATGTTGTACAGTGATAGTGCTGCTATTGATATGGAGTGTCCTTCATTGGAACACTTGTATGTGGAAGACTGCCCTCATGTTCCGGCCTCTGCTTATGACTTCGGGAGCAATAACCAAGTCATACTCAACGATCCACGGCATTACGCATCTCAGCAAGCCACAAAATTTGGCACACCCCTATCAGCATGGGATACTCTCATCAAGTCACGTCTCAAGGTCGGGGAAAAGATCTTAACATCGATGGAGCCTGAAATGATGCAGAGGGCGGTTGAATTCACGGGGAAGATGTCTCAAATCATACAGACGAAGCCCGAATTTGCAAAGAAGTTTTTACCTAGTTCGTTAGCGAAGCCTGAATTGTTGGAGATGATGCCTAGACTGATGGAGTTGCTGAATGGAAGTTTGACAGAGAAGAATCCTGAATCGATGGAGAATCTTATAGCGTTGGCGAATATCACTCCTGATCTGATGAATCATATACCTGCTGAGATTAGTGAAATGATGGATCTGATGACTAAATTGATGGAGGATACGAACTTTATCAAGTCCTTGACTGGAATCGTGGAGAACATCCCTGGAATCATGAACTTGCCTCGAATCATAGAGGACTTTCCTCGATTGTTTGAGATTTTGCCAATGACTGGATCGGTGGAGAATATGCCTGGAATCATGGAGGACTTTGCTCAATTGATTGAGAATTTGCCTCAGATGGCTGAATCGTTGAAGGACTTTGCTCAATCGGTTGAGAATTTTCTGAATTTGCCTCAAATGACTGAATGGATGGGAATCATGGAGAACTTATCTCAGATGACTGAATAG

Protein Analysis

977

Amino Acids

110.95

Weight (kDa)

5.21

Isoelectric Point (pI)

44.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 231 - 290 1.4e-09 Leucine rich repeat
LRR_14 PF23598 245 - 369 1.1e-10 Leucine-rich repeat region
LRR_RPS2 PF23247 530 - 672 1.2e-18 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 620 - 730 2e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1903
Acc36I ACCTGC 3 cut(s) 1672, 1903, 2605
AccB7I CCANNNNNTGG 1 cut(s) 2756
AccI GTMKAC 2 cut(s) 92, 142
AccII CGCG 3 cut(s) 232, 666, 1205
AciI CCGC 1 cut(s) 2381
AclWI GGATC 7 cut(s) 395, 401, 733, 2115, 2240, 2628, 2767
AcoI YGGCCR 2 cut(s) 453, 608
AcuI CTGAAG 5 cut(s) 579, 696, 1511, 1643, 1691
AfaI GTAC 6 cut(s) 31, 935, 1686, 2007, 2093, 2123
AfeI AGCGCT 1 cut(s) 1911
AfiI CCNNNNNNNGG 7 cut(s) 943, 981, 1222, 1703, 2023, 2101, 2756
AhdI GACNNNNNGTC 1 cut(s) 313
AhlI ACTAGT 2 cut(s) 764, 797
AjiI CACGTC 2 cut(s) 614, 2324
AjnI CCWGG 3 cut(s) 1002, 2689, 2776
AjuI GAANNNNNNNTTGG 4 cut(s) 305, 337, 1779, 1811
Alw21I GWGCWC 2 cut(s) 570, 2061
Alw26I GTCTC 6 cut(s) 1266, 1662, 1732, 1958, 2330, 2409
AlwI GGATC 7 cut(s) 395, 401, 733, 2115, 2240, 2628, 2767
AlwNI CAGNNNCTG 1 cut(s) 1741
Aor51HI AGCGCT 1 cut(s) 1911
AoxI GGCC 4 cut(s) 453, 608, 628, 2199
ApeKI GCWGC 3 cut(s) 209, 236, 2135
Asp700I GAANNNNTTC 1 cut(s) 1760
AspA2I CCTAGG 1 cut(s) 2095
AspLEI GCGC 1 cut(s) 1912
AspS9I GGNCC 2 cut(s) 1511, 1883
AsuC2I CCSGG 1 cut(s) 530
AsuHPI GGTGA 5 cut(s) 13, 821, 1057, 1411, 1460
AvaII GGWCC 2 cut(s) 1511, 1883
AvrII CCTAGG 1 cut(s) 2095
BaeGI GKGCMC 1 cut(s) 175
BalI TGGCCA 2 cut(s) 455, 610
BanII GRGCYC 1 cut(s) 2061
BauI CACGAG 1 cut(s) 1457
BbsI GAAGAC 5 cut(s) 354, 375, 567, 2075, 2187
Bbv12I GWGCWC 2 cut(s) 570, 2061
BbvI GCAGC 3 cut(s) 221, 223, 2122
BceAI ACGGC 2 cut(s) 233, 2267
BcgI CGANNNNNNTGC 2 cut(s) 92, 126
BciT130I CCWGG 3 cut(s) 1004, 2691, 2778
BciVI GTATCC 3 cut(s) 526, 2299, 2638
BclI TGATCA 1 cut(s) 1536
BcnI CCSGG 1 cut(s) 530
BcoDI GTCTC 6 cut(s) 1266, 1662, 1732, 1958, 2330, 2409
BcuI ACTAGT 2 cut(s) 764, 797
BfoI RGCGCY 1 cut(s) 1913
BfuAI ACCTGC 3 cut(s) 1672, 1903, 2605
BfuI GTATCC 3 cut(s) 526, 2299, 2638
BglII AGATCT 2 cut(s) 1343, 2343
BisI GCNGC 3 cut(s) 210, 237, 2136
BlnI CCTAGG 1 cut(s) 2095
BlsI GCNGC 3 cut(s) 211, 238, 2137
Bme1390I CCNGG 4 cut(s) 530, 1004, 2691, 2778
Bme18I GGWCC 2 cut(s) 1511, 1883
BmeRI GACNNNNNGTC 1 cut(s) 313
BmgBI CACGTC 2 cut(s) 614, 2324
BmgT120I GGNCC 2 cut(s) 1511, 1883
BmiI GGNNCC 4 cut(s) 8, 1513, 1618, 2361
BmrFI CCNGG 4 cut(s) 530, 1004, 2691, 2778
BmsI GCATC 8 cut(s) 272, 504, 1221, 1504, 1609, 2270, 2361, 2475
BpiI GAAGAC 5 cut(s) 354, 375, 567, 2075, 2187
BpmI CTGGAG 3 cut(s) 1123, 1625, 1772
Bpu10I CCTNAGC 1 cut(s) 833
BpuEI CTTGAG 2 cut(s) 932, 2312
BpuMI CCSGG 1 cut(s) 530
Bsa29I ATCGAT 2 cut(s) 2354, 2540
BsaBI GATNNNNATC 2 cut(s) 392, 2900
BsaI GGTCTC 1 cut(s) 1662
BsaJI CCNNGG 6 cut(s) 844, 1003, 1471, 2095, 2249, 2689
BsaXI ACNNNNNCTCC 2 cut(s) 1011, 1041
Bsc4I CCNNNNNNNGG 7 cut(s) 943, 981, 1222, 1703, 2023, 2101, 2756
Bse1I ACTGG 4 cut(s) 1106, 1746, 2674, 2761
Bse3DI GCAATG 2 cut(s) 1091, 1905
Bse8I GATNNNNATC 2 cut(s) 392, 2900
BseBI CCWGG 3 cut(s) 1004, 2691, 2778
BseCI ATCGAT 2 cut(s) 2354, 2540
BseDI CCNNGG 6 cut(s) 844, 1003, 1471, 2095, 2249, 2689
BseJI GATNNNNATC 2 cut(s) 392, 2900
BseLI CCNNNNNNNGG 7 cut(s) 943, 981, 1222, 1703, 2023, 2101, 2756
BseMI GCAATG 2 cut(s) 1091, 1905
BseNI ACTGG 4 cut(s) 1106, 1746, 2674, 2761
BseRI GAGGAG 2 cut(s) 19, 1120
BseSI GKGCMC 1 cut(s) 175
BseXI GCAGC 3 cut(s) 221, 223, 2122
Bsh1236I CGCG 3 cut(s) 232, 666, 1205
BshFI GGCC 4 cut(s) 455, 610, 630, 2201
BshVI ATCGAT 2 cut(s) 2354, 2540
BsiHKAI GWGCWC 2 cut(s) 570, 2061
BsiSI CCGG 2 cut(s) 529, 2198
BslFI GGGAC 2 cut(s) 1112, 1497
BslI CCNNNNNNNGG 7 cut(s) 943, 981, 1222, 1703, 2023, 2101, 2756
BsmAI GTCTC 6 cut(s) 1266, 1662, 1732, 1958, 2330, 2409
BsmBI CGTCTC 1 cut(s) 2330
BsmFI GGGAC 2 cut(s) 1112, 1497
BsmI GAATGC 1 cut(s) 275
BsnI GGCC 4 cut(s) 455, 610, 630, 2201
Bso31I GGTCTC 1 cut(s) 1662
Bsp1286I GDGCHC 3 cut(s) 175, 570, 2061
Bsp1407I TGTACA 1 cut(s) 2121
Bsp19I CCATGG 1 cut(s) 1471
Bsp68I TCGCGA 1 cut(s) 666
BspACI CCGC 1 cut(s) 2381
BspANI GGCC 4 cut(s) 455, 610, 630, 2201
BspDI ATCGAT 2 cut(s) 2354, 2540
BspFNI CGCG 3 cut(s) 232, 666, 1205
BspHI TCATGA 2 cut(s) 648, 2697
BspLI GGNNCC 4 cut(s) 8, 1513, 1618, 2361
BspMI ACCTGC 3 cut(s) 1672, 1903, 2605
BspPI GGATC 7 cut(s) 395, 401, 733, 2115, 2240, 2628, 2767
BspQI GCTCTTC 1 cut(s) 672
BspTNI GGTCTC 1 cut(s) 1662
BsrDI GCAATG 2 cut(s) 1091, 1905
BsrGI TGTACA 1 cut(s) 2121
BsrI ACTGG 4 cut(s) 1106, 1746, 2674, 2761
BssECI CCNNGG 6 cut(s) 844, 1003, 1471, 2095, 2249, 2689
BssSI CACGAG 1 cut(s) 1457
BssT1I CCWWGG 3 cut(s) 844, 1471, 2095
Bst2BI CACGAG 1 cut(s) 1457
Bst2UI CCWGG 3 cut(s) 1004, 2691, 2778
Bst4CI ACNGT 6 cut(s) 1077, 1181, 1919, 1934, 2074, 2126
Bst6I CTCTTC 2 cut(s) 672, 1961
BstAUI TGTACA 1 cut(s) 2121
BstC8I GCNNGC 7 cut(s) 234, 479, 589, 668, 1082, 2044, 2271
BstDSI CCRYGG 2 cut(s) 1471, 2249
BstFNI CGCG 3 cut(s) 232, 666, 1205
BstH2I RGCGCY 1 cut(s) 1913
BstHHI GCGC 1 cut(s) 1912
BstMAI GTCTC 6 cut(s) 1266, 1662, 1732, 1958, 2330, 2409
BstMWI GCNNNNNNNGC 2 cut(s) 158, 1895
BstNI CCWGG 3 cut(s) 1004, 2691, 2778
BstSCI CCNGG 4 cut(s) 528, 1002, 2689, 2776
BstSLI GKGCMC 1 cut(s) 175
BstUI CGCG 3 cut(s) 232, 666, 1205
BstV1I GCAGC 3 cut(s) 221, 223, 2122
BstV2I GAAGAC 5 cut(s) 354, 375, 567, 2075, 2187
BstX2I RGATCY 5 cut(s) 738, 1343, 2107, 2343, 2620
BstYI RGATCY 5 cut(s) 738, 1343, 2107, 2343, 2620
Bsu15I ATCGAT 2 cut(s) 2354, 2540
BsuI GTATCC 3 cut(s) 526, 2299, 2638
BsuRI GGCC 4 cut(s) 455, 610, 630, 2201
BsuTUI ATCGAT 2 cut(s) 2354, 2540
BtgI CCRYGG 2 cut(s) 1471, 2249
BtrI CACGTC 2 cut(s) 614, 2324
BtsIMutI CAGTG 3 cut(s) 456, 1017, 2131
BtuMI TCGCGA 1 cut(s) 666
BveI ACCTGC 3 cut(s) 1672, 1903, 2605
Cac8I GCNNGC 7 cut(s) 234, 479, 589, 668, 1082, 2044, 2271
CaiI CAGNNNCTG 1 cut(s) 1741
CciI TCATGA 2 cut(s) 648, 2697
CfoI GCGC 1 cut(s) 1912
Cfr13I GGNCC 2 cut(s) 1511, 1883
ClaI ATCGAT 2 cut(s) 2354, 2540
CseI GACGC 1 cut(s) 1211
Csp6I GTAC 6 cut(s) 30, 934, 1685, 2006, 2092, 2122
CspCI CAANNNNNGTGG 4 cut(s) 600, 635, 2263, 2298
CviQI GTAC 6 cut(s) 30, 934, 1685, 2006, 2092, 2122
DriI GACNNNNNGTC 1 cut(s) 313
EaeI YGGCCR 2 cut(s) 453, 608
Eam1104I CTCTTC 2 cut(s) 672, 1961
Eam1105I GACNNNNNGTC 1 cut(s) 313
EarI CTCTTC 2 cut(s) 672, 1961
Ecl136II GAGCTC 1 cut(s) 2059
Eco130I CCWWGG 3 cut(s) 844, 1471, 2095
Eco24I GRGCYC 1 cut(s) 2061
Eco31I GGTCTC 1 cut(s) 1662
Eco32I GATATC 1 cut(s) 1050
Eco47I GGWCC 2 cut(s) 1511, 1883
Eco47III AGCGCT 1 cut(s) 1911
Eco53kI GAGCTC 1 cut(s) 2059
Eco57I CTGAAG 5 cut(s) 579, 696, 1511, 1643, 1691
EcoICRI GAGCTC 1 cut(s) 2059
EcoRI GAATTC 2 cut(s) 1032, 2387
EcoRII CCWGG 3 cut(s) 1002, 2689, 2776
EcoRV GATATC 1 cut(s) 1050
EcoT14I CCWWGG 3 cut(s) 844, 1471, 2095
EcoT22I ATGCAT 2 cut(s) 275, 519
EcoT38I GRGCYC 1 cut(s) 2061
ErhI CCWWGG 3 cut(s) 844, 1471, 2095
Esp3I CGTCTC 1 cut(s) 2330
FalI AAGNNNNNCTT 6 cut(s) 67, 99, 305, 337, 1829, 1861
FaqI GGGAC 2 cut(s) 1112, 1497
FauNDI CATATG 1 cut(s) 1874
FbaI TGATCA 1 cut(s) 1536
FblI GTMKAC 2 cut(s) 92, 142
Fnu4HI GCNGC 3 cut(s) 210, 237, 2136
FriOI GRGCYC 1 cut(s) 2061
Fsp4HI GCNGC 3 cut(s) 210, 237, 2136
GlaI GCGC 1 cut(s) 1911
GluI GCNGC 3 cut(s) 210, 237, 2136
GsuI CTGGAG 3 cut(s) 1123, 1625, 1772
HaeII RGCGCY 1 cut(s) 1913
HaeIII GGCC 4 cut(s) 455, 610, 630, 2201
HapII CCGG 2 cut(s) 529, 2198
HgaI GACGC 1 cut(s) 1211
HhaI GCGC 1 cut(s) 1912
Hin6I GCGC 1 cut(s) 1910
HinP1I GCGC 1 cut(s) 1910
HincII GTYRAC 3 cut(s) 93, 954, 1313
HindII GTYRAC 3 cut(s) 93, 954, 1313
HindIII AAGCTT 1 cut(s) 908
HpaII CCGG 2 cut(s) 529, 2198
HphI GGTGA 5 cut(s) 13, 821, 1057, 1411, 1460
Hpy166II GTNNAC 5 cut(s) 93, 143, 770, 954, 1313
Hpy8I GTNNAC 5 cut(s) 93, 143, 770, 954, 1313
Hpy99I CGWCG 2 cut(s) 618, 785
HpyAV CCTTC 9 cut(s) 112, 620, 1180, 1215, 1618, 1666, 1799, 2166, 2833
HpyCH4III ACNGT 6 cut(s) 1077, 1181, 1919, 1934, 2074, 2126
HpyCH4IV ACGT 2 cut(s) 613, 2323
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 1895
HpySE526I ACGT 2 cut(s) 613, 2323
HspAI GCGC 1 cut(s) 1910
Ksp22I TGATCA 1 cut(s) 1536
LguI GCTCTTC 1 cut(s) 672
LmnI GCTCC 5 cut(s) 6, 702, 1894, 2220, 2359
Lsp1109I GCAGC 3 cut(s) 221, 223, 2122
LweI GCATC 8 cut(s) 272, 504, 1221, 1504, 1609, 2270, 2361, 2475
MaeII ACGT 2 cut(s) 613, 2323
MaeIII GTNAC 9 cut(s) 791, 1156, 1374, 1399, 1479, 1528, 1716, 1731, 2319
MfeI CAATTG 2 cut(s) 147, 2801
MflI RGATCY 5 cut(s) 738, 1343, 2107, 2343, 2620
MhlI GDGCHC 3 cut(s) 175, 570, 2061
MlsI TGGCCA 2 cut(s) 455, 610
MluNI TGGCCA 2 cut(s) 455, 610
MlyI GAGTC 4 cut(s) 98, 302, 786, 1117
MmeI TCCRAC 3 cut(s) 969, 1223, 2457
Mox20I TGGCCA 2 cut(s) 455, 610
Mph1103I ATGCAT 2 cut(s) 275, 519
MroXI GAANNNNTTC 1 cut(s) 1760
MscI TGGCCA 2 cut(s) 455, 610
MseI TTAA 4 cut(s) 903, 980, 1445, 2348
MslI CAYNNNNRTG 4 cut(s) 268, 1370, 1457, 2172
Msp20I TGGCCA 2 cut(s) 455, 610
MspI CCGG 2 cut(s) 529, 2198
MspR9I CCNGG 4 cut(s) 530, 1004, 2691, 2778
MunI CAATTG 2 cut(s) 147, 2801
Mva1269I GAATGC 1 cut(s) 275
MvaI CCWGG 3 cut(s) 1004, 2691, 2778
MvnI CGCG 3 cut(s) 232, 666, 1205
MwoI GCNNNNNNNGC 2 cut(s) 158, 1895
NciI CCSGG 1 cut(s) 530
NcoI CCATGG 1 cut(s) 1471
NdeI CATATG 1 cut(s) 1874
NlaIV GGNNCC 4 cut(s) 8, 1513, 1618, 2361
NmuCI GTSAC 2 cut(s) 1399, 2319
NruI TCGCGA 1 cut(s) 666
NsiI ATGCAT 2 cut(s) 275, 519
PagI TCATGA 2 cut(s) 648, 2697
PaqCI CACCTGC 1 cut(s) 1903
PciSI GCTCTTC 1 cut(s) 672
PcsI WCGNNNNNNNCGW 3 cut(s) 576, 619, 663
PctI GAATGC 1 cut(s) 275
PdmI GAANNNNTTC 1 cut(s) 1760
PflMI CCANNNNNTGG 1 cut(s) 2756
PfoI TCCNGGA 1 cut(s) 528
PkrI GCNGC 3 cut(s) 211, 238, 2137
PleI GAGTC 4 cut(s) 97, 302, 785, 1116
PpsI GAGTC 4 cut(s) 97, 302, 785, 1116
Psp124BI GAGCTC 1 cut(s) 2061
Psp6I CCWGG 3 cut(s) 1002, 2689, 2776
PspGI CCWGG 3 cut(s) 1002, 2689, 2776
PspN4I GGNNCC 4 cut(s) 8, 1513, 1618, 2361
PspPI GGNCC 2 cut(s) 1511, 1883
PsrI GAACNNNNNNTAC 2 cut(s) 991, 1023
PstNI CAGNNNCTG 1 cut(s) 1741
PsuI RGATCY 5 cut(s) 738, 1343, 2107, 2343, 2620
RruI TCGCGA 1 cut(s) 666
RsaI GTAC 6 cut(s) 31, 935, 1686, 2007, 2093, 2123
RsaNI GTAC 6 cut(s) 30, 934, 1685, 2006, 2092, 2122
RseI CAYNNNNRTG 4 cut(s) 268, 1370, 1457, 2172
SacI GAGCTC 1 cut(s) 2061
SalI GTCGAC 1 cut(s) 91
SapI GCTCTTC 1 cut(s) 672
SaqAI TTAA 4 cut(s) 903, 980, 1445, 2348
SatI GCNGC 3 cut(s) 210, 237, 2136
Sau96I GGNCC 2 cut(s) 1511, 1883
SchI GAGTC 4 cut(s) 98, 302, 786, 1117
ScrFI CCNGG 4 cut(s) 530, 1004, 2691, 2778
SduI GDGCHC 3 cut(s) 175, 570, 2061
SfaNI GCATC 8 cut(s) 272, 504, 1221, 1504, 1609, 2270, 2361, 2475
SinI GGWCC 2 cut(s) 1511, 1883
SmiMI CAYNNNNRTG 4 cut(s) 268, 1370, 1457, 2172
SmlI CTYRAG 2 cut(s) 911, 2327
SmoI CTYRAG 2 cut(s) 911, 2327
SpeI ACTAGT 2 cut(s) 764, 797
SsiI CCGC 1 cut(s) 2381
SspI AATATT 2 cut(s) 1291, 1337
SstI GAGCTC 1 cut(s) 2061
StyD4I CCNGG 4 cut(s) 528, 1002, 2689, 2776
StyI CCWWGG 3 cut(s) 844, 1471, 2095
TaaI ACNGT 6 cut(s) 1077, 1181, 1919, 1934, 2074, 2126
TaiI ACGT 2 cut(s) 616, 2326
TatI WGTACW 2 cut(s) 2005, 2121
Tru1I TTAA 4 cut(s) 903, 980, 1445, 2348
Tru9I TTAA 4 cut(s) 903, 980, 1445, 2348
TscAI CASTG 3 cut(s) 456, 1017, 2131
TseFI GTSAC 2 cut(s) 1399, 2319
TseI GCWGC 3 cut(s) 209, 236, 2135
Tsp45I GTSAC 2 cut(s) 1399, 2319
TspDTI ATGAA 6 cut(s) 39, 941, 1856, 2148, 2600, 2714
TspGWI ACGGA 2 cut(s) 585, 1962
TspRI CASTG 3 cut(s) 456, 1017, 2131
Van91I CCANNNNNTGG 1 cut(s) 2756
VpaK11BI GGWCC 2 cut(s) 1511, 1883
XcmI CCANNNNNNNNNTGG 1 cut(s) 1522
XmaJI CCTAGG 1 cut(s) 2095
XmiI GTMKAC 2 cut(s) 92, 142
XmnI GAANNNNTTC 1 cut(s) 1760
Zsp2I ATGCAT 2 cut(s) 275, 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.