FvH4_5g27640

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000085
Physical Location & Seq
Forward (+)
18837157 .. 18842676
5520 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g27640.t1

Sequence Viewer

Length: 3495 bp
ATGGCCTTGAGCACCCAAAGAGCCTCTGCATCTCAGACTTCATCAGCTGAATCATCAGTTCCTCGCAGGAAGAATGATGTTTTTCTGAGTTTCAGGGGTGTAGACACTCGCAGGACGATTGTCTCCTCTTTATACCATGAGTTGCAGAACAGAGAAGTTGAAACGTTCATGGATGACAAAGGGCTTATCAGAGGGACTATTATTTCCTCGAGTCTTCTGAGAGAAATCAATGAATCTAGATTGGCAATCATTGTCCTTTCACCAAACTATGCTTATTCTAAATGGTGTTTGGACGAATTGACAATGATCATTCAGTGCATGGAATATAATAGCACAACAGTCATCCCAATCTTTTATGATGTGGATCCTTCTGATGTGCGACATCAGAAAGGGAGCTTTGCAAGAGCCTTCACTACGCACCAAGAAAGGCTAGGGCAAGACGATGAGTACCCATATAAGTTGAACGAATGGAGAGTTGCTTTAACAAAAGTTGCCGGTCTCTCTGGCTTGGAATCAAAAAAATATAGTTCTGACGTGGAACTTGTCAAAGACGCTGTGGATGTTGTGTGCAGTAAATTACAACATGTTGAGTGGGCATTGTTGTCTACTGGAGATTTTGTAGCATTTGAAGCAACAAGGCGAGCCATGAACAAGGTAATGAAGGCAATGCGAGATGATGAGATCACTGCCATTGGGGTATATGGGATGGGAGGCATTGGCAAGACATGTATGGTGAAACATGTGGGTGCACATGCCCGAAAACTTGGTCTTTTTAATCATGTAATTATGGCTGTCATATCCCAAACACCAAACTTGAGAAATATTCAAGGAACACTGGCCGATCTGCTGGGATTAAAGTTGTTCGAGGAGACAGAATTCGGAAGAGCAGTTAGATTGATGGAGAAGATAATGAAAGGAAATAGGATCCTCATATTCTTGGACGACATTTGGGAGAGAATAGATTTGTCTAGCATTGGAATACCGAGCCATGATGAGCTTCAAAAGTGCAGTTCCAAAATCATATTCACCACAAGGAGATTGAATGTTTGTCATACCATGGAGAGCCAAGAAAACATTCCTCTCCAAGTCTTATCTGAAGAAGATGCTTGGAATCTATTTGTGAAGAAAACAAGAAAGTCTTTTCATGAATCAACAAACTTATATGATGTAGGGCGGAGAGTAGCAAAAGAATGTGCTGGTCTCCCAGTTGCATTAGTAGCAGTTGCGAGGGCACTTGGAGATAAAGATTTAGATGATTGGACAGAAGCAGCTCGACGTCTAAAGGCTGCTCAACCTGCCAACTATGAAGATGAGCGAGAGGTGTCTAGATGTATAGAGTTAAGCTATAATTATTTGAGACCTGATGATGACAACATTGCCAAGTTATTCTTCTTGCTTTGCTGTCTATTCCCTGAAGATTCTGATATTGAGGTCGAAGACTTGTTGGTGTATGCAATTGGGAAAGGACTGTTTCAAGATGCTGATACAATCAAAGAAGCCAGAGCCAAAGCTCATTCAGTGGTCAAGTACCTGAAAGCTTCTAGCTTGCTTTTGGACAGTGCATACTGTGGATTTGTGAAGATGCATGACGTAATTCGAGATGTGGCCATATCAATCTCATTATCGGAAGATGGATCACGGTTTTTGGTTAAAGCTGGTTGTGAATTAAAGGATTGGCCAGCTATTAATATTGCAGATAAAGGCTACTCTGCAGTCTCACTAATGAGAAATGAAATTCAGAAGCTTCCCAACAAGTTGGTATGTTCAAAACTCCAGATTTTATTACTACAGAGCAATAGTAATATAAATGAGATTCCAAATCCATTTTTTCAAAGTCCCAATGAATTGAGGGTCTTAGATCTTAGCCAGACTAGTATTTCATTACTACCCGCCTCATTAAGTCTGCTAACCAACCTCCAAGCTTTGTGTTTAGATGATTGCAAGAAAATGCATGATATTTCCATACTCGGAAAGCTAAAGAACCTTCAGATTCTTAGTATGAAGAACCTTAGAGGAATTTGTGATAGAGAACTGCCGAAAGAAATGGCGCAGTTGGCCAAACTAAAGTTGTTAGACATCACGAATGCATGTTTTGTAACAGTGCCCTCTGATGTGATATTAAACTTGAATGGATTAGAAGAACTGTACATGAAATGCGACTTCTGTGACTGGGGTAGTAGAATAGTCACGGGCAGTTTATTTTCTTTTGCTTTTGGCACTGCCAAGAATGCTAGCTTTGATGAGTTAACAGGCTTGTCACGTTTAAACAGTTTGAAAGTTCACATATCTGATGTGAAATGCTTGCCTCGTAACGTTAGTTGCATTCCGAATTGGGTCAACTTCAATATCTGTATCAGCAGAAAGGAATATATGAAACAGTCCTATTATGGCCGTTTTTTTGAGAGGAAACTAACTGTCAAGTGGGATCAACGTCAGCATCTCTTTCGGTATGTGACTCTAAACGCAAGCACGAAGAGTTTACCAGATTGGTTTATCAATGTGGTGACAGAGAAAACAGAGATTCTGGAGTACATAGGATACAGTGGCTTAGAGGACATTCTTGTGGAATACGACCTTGGGAGATTACATGTGCTGAAGTGCCTTGCTGTAATTGAGTGCCAAGTGGATGGAGTGTTGATGAACTCAACAACAAGAAGGATTCAAAATGAACCAGTATTCGAGAGCTTGATAGATTTGTATGTTGAAAGAGTCTATGGGCTGGAGGATTTATGTATTGGTGAGTTACCACCTGGGTCTCTATGCAATCTTCAGTTGTTAAATGTGAGAAACTGCCCTCATTTGGTGGATGCACTTTTACAATCAAATTTGTTGCTCAGGCTACAAAATCTGGAAATACTACATTGTGAGCAAGTGGAGGATGCGAGAGGAACCAAAATGGAACATGTGTTTAGATTTGAAGAGCTGGAGGGTCAACAAACCGTCTTAACGAAATTGAGAGAGATAACTTTGTGTAATCTTGTGAGCCTTGAAAGAATATGGATCGGTTATCCTCCAAATGGAGCATTCTGTAATCTTAACAGTTTGGCAATTCGTGGCTGCAACAAACTGAAATATCTCTTGGCCTATGGTGCTACTATATCTTTTTCGCAATTGGAGCTCCTTTGGGTGGATGACTGCTCTGACTTGAAAAGCCTTTTTTCACCAACCTTGGGACAAGAGACCAAGGTAGTTATTCCCAAATTGAAGGAAATGTATTTGAGTAGTCTTCCACAGCTCTCAGGGTTCTTCAGTTGCCTCTATCAAGAAGCTACTACTCAGCTTCTCTGCCCTTCATTGGAACACTTGTATGTCTATGGGTGCCCCAAACTTTCGAACCTTGCTGCTTCTAACTTCCACAGCAGGAAAGAAGTCCAAGCCAATGATGAGCGGCATTTTGCGCTGATATTTGAGAGATTGTTCCACCGCCAATTCAACCCAGAAGAGTTTCCTCAGTTCTTCTCCAAAAAAGGAAGTGATTTCTCAATTGGATCTTGA

Protein Analysis

1165

Amino Acids

131.93

Weight (kDa)

6.1

Isoelectric Point (pI)

42.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 26 - 195 1.4e-45 TIR domain
TIR_2 PF13676 27 - 124 1.7e-14 TIR domain
NB-ARC PF00931 215 - 377 4e-29 NB-ARC domain
LRR_8 PF13855 590 - 647 3.7e-06 Leucine rich repeat
LRR_14 PF23598 599 - 670 1e-05 Leucine-rich repeat region
LRR_RPS2 PF23247 905 - 1046 7.5e-19 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1006 - 1113 5.1e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1279
Acc36I ACCTGC 1 cut(s) 1303
AccB1I GGYRCC 1 cut(s) 3318
AccBSI CCGCTC 1 cut(s) 3388
AccI GTMKAC 2 cut(s) 102, 605
AciI CCGC 4 cut(s) 1174, 1890, 3388, 3424
AclI AACGTT 2 cut(s) 164, 2313
AclWI GGATC 7 cut(s) 359, 372, 919, 932, 1642, 2433, 3008
AcoI YGGCCR 5 cut(s) 837, 1605, 1676, 2055, 2389
AcsI RAATTY 4 cut(s) 875, 1734, 2016, 2824
AcuI CTGAAG 6 cut(s) 1116, 1434, 1970, 2615, 2753, 3232
AcyI GRCGYC 1 cut(s) 1276
AfaI GTAC 4 cut(s) 449, 1529, 2147, 2531
AfiI CCNNNNNNNGG 5 cut(s) 2800, 3017, 3127, 3170, 3295
AflIII ACRYGT 5 cut(s) 583, 725, 739, 2587, 2902
AhlI ACTAGT 1 cut(s) 1871
AjiI CACGTC 1 cut(s) 535
AjnI CCWGG 1 cut(s) 2749
Alw21I GWGCWC 3 cut(s) 14, 751, 3120
Alw26I GTCTC 8 cut(s) 127, 503, 863, 1205, 1351, 1720, 2760, 3173
Alw44I GTGCAC 1 cut(s) 747
AlwI GGATC 7 cut(s) 359, 372, 919, 932, 1642, 2433, 3008
Ama87I CYCGRG 1 cut(s) 208
AoxI GGCC 7 cut(s) 3, 837, 1605, 1676, 2055, 2389, 3081
ApaLI GTGCAC 1 cut(s) 747
ApeKI GCWGC 4 cut(s) 1268, 1286, 3057, 3341
ApoI RAATTY 4 cut(s) 875, 1734, 2016, 2824
ArsI GACNNNNNNTTYG 2 cut(s) 751, 783
AseI ATTAAT 1 cut(s) 1686
Asp700I GAANNNNTTC 2 cut(s) 1074, 3444
AspLEI GCGC 2 cut(s) 2050, 3400
AsuHPI GGTGA 6 cut(s) 252, 745, 1018, 2515, 2750, 3153
AsuII TTCGAA 1 cut(s) 3332
AsuNHI GCTAGC 1 cut(s) 2231
AvaI CYCGRG 1 cut(s) 208
BaeGI GKGCMC 4 cut(s) 751, 1234, 2106, 3323
BalI TGGCCA 3 cut(s) 1607, 1678, 2057
BamHI GGATCC 2 cut(s) 364, 924
BanI GGYRCC 1 cut(s) 3318
BanII GRGCYC 1 cut(s) 3120
BbsI GAAGAC 3 cut(s) 206, 1443, 3218
Bbv12I GWGCWC 3 cut(s) 14, 751, 3120
BbvI GCAGC 4 cut(s) 1273, 1280, 3044, 3328
BccI CCATC 4 cut(s) 700, 892, 1625, 2621
BceAI ACGGC 1 cut(s) 2376
BcgI CGANNNNNNTGC 2 cut(s) 2426, 2460
BciT130I CCWGG 1 cut(s) 2751
BciVI GTATCC 1 cut(s) 2531
BclI TGATCA 1 cut(s) 306
BcoDI GTCTC 8 cut(s) 127, 503, 863, 1205, 1351, 1720, 2760, 3173
BcuI ACTAGT 1 cut(s) 1871
BfaI CTAG 7 cut(s) 237, 431, 969, 1326, 1542, 1872, 2232
BfmI CTRYAG 2 cut(s) 1710, 1787
BfuAI ACCTGC 1 cut(s) 1303
BfuI GTATCC 1 cut(s) 2531
BglII AGATCT 1 cut(s) 1858
BisI GCNGC 5 cut(s) 1269, 1287, 3058, 3342, 3389
BlsI GCNGC 5 cut(s) 1270, 1288, 3059, 3343, 3390
Bme1390I CCNGG 1 cut(s) 2751
BmeT110I CYCGRG 1 cut(s) 208
BmgBI CACGTC 1 cut(s) 535
BmiI GGNNCC 4 cut(s) 366, 926, 2890, 3320
BmrFI CCNGG 1 cut(s) 2751
BmrI ACTGGG 2 cut(s) 1199, 2179
BmsI GCATC 7 cut(s) 38, 1093, 1468, 1572, 2446, 2797, 2869
BmtI GCTAGC 1 cut(s) 2235
BmuI ACTGGG 2 cut(s) 1199, 2179
BoxI GACNNNNGTC 1 cut(s) 119
BpiI GAAGAC 3 cut(s) 206, 1443, 3218
BpmI CTGGAG 5 cut(s) 630, 1757, 2546, 2741, 2945
Bpu10I CCTNAGC 1 cut(s) 2834
Bpu14I TTCGAA 1 cut(s) 3332
BpuEI CTTGAG 2 cut(s) 28, 835
BsaBI GATNNNNATC 1 cut(s) 363
BsaHI GRCGYC 1 cut(s) 1276
BsaI GGTCTC 5 cut(s) 503, 1205, 1351, 2760, 3173
BsaJI CCNNGG 5 cut(s) 1056, 2575, 2750, 3168, 3183
BsaXI ACNNNNNCTCC 2 cut(s) 603, 633
Bsc4I CCNNNNNNNGG 5 cut(s) 2800, 3017, 3127, 3170, 3295
Bse118I RCCGGY 1 cut(s) 494
Bse1I ACTGG 5 cut(s) 613, 840, 1205, 2174, 2672
Bse3DI GCAATG 2 cut(s) 672, 1374
Bse8I GATNNNNATC 1 cut(s) 363
BseBI CCWGG 1 cut(s) 2751
BseDI CCNNGG 5 cut(s) 1056, 2575, 2750, 3168, 3183
BseGI GGATG 8 cut(s) 178, 342, 565, 711, 2632, 2812, 2884, 3136
BseJI GATNNNNATC 1 cut(s) 363
BseLI CCNNNNNNNGG 5 cut(s) 2800, 3017, 3127, 3170, 3295
BseMI GCAATG 2 cut(s) 672, 1374
BseMII CTCAG 7 cut(s) 47, 77, 209, 2848, 3252, 3290, 3464
BseNI ACTGG 5 cut(s) 613, 840, 1205, 2174, 2672
BseRI GAGGAG 2 cut(s) 115, 881
BseSI GKGCMC 4 cut(s) 751, 1234, 2106, 3323
BseXI GCAGC 4 cut(s) 1273, 1280, 3044, 3328
BseYI CCCAGC 1 cut(s) 847
BsgI GTGCAG 2 cut(s) 589, 1027
BshFI GGCC 7 cut(s) 5, 839, 1607, 1678, 2057, 2391, 3083
BshNI GGYRCC 1 cut(s) 3318
BsiHKAI GWGCWC 3 cut(s) 14, 751, 3120
BsiHKCI CYCGRG 1 cut(s) 208
BsiSI CCGG 1 cut(s) 495
BslFI GGGAC 3 cut(s) 208, 1821, 3186
BslI CCNNNNNNNGG 5 cut(s) 2800, 3017, 3127, 3170, 3295
BsmAI GTCTC 8 cut(s) 127, 503, 863, 1205, 1351, 1720, 2760, 3173
BsmFI GGGAC 3 cut(s) 208, 1821, 3186
BsmI GAATGC 4 cut(s) 2089, 2233, 2322, 3023
BsnI GGCC 7 cut(s) 5, 839, 1607, 1678, 2057, 2391, 3083
Bso31I GGTCTC 5 cut(s) 503, 1205, 1351, 2760, 3173
BsoBI CYCGRG 1 cut(s) 208
Bsp119I TTCGAA 1 cut(s) 3332
Bsp1286I GDGCHC 6 cut(s) 14, 751, 1234, 2106, 3120, 3323
Bsp1407I TGTACA 1 cut(s) 2145
Bsp19I CCATGG 1 cut(s) 1056
BspACI CCGC 4 cut(s) 1174, 1890, 3388, 3424
BspANI GGCC 7 cut(s) 5, 839, 1607, 1678, 2057, 2391, 3083
BspCNI CTCAG 7 cut(s) 46, 78, 210, 2847, 3251, 3289, 3463
BspHI TCATGA 1 cut(s) 1144
BspLI GGNNCC 4 cut(s) 366, 926, 2890, 3320
BspMAI CTGCAG 1 cut(s) 1714
BspMI ACCTGC 1 cut(s) 1303
BspOI GCTAGC 1 cut(s) 2235
BspPI GGATC 7 cut(s) 359, 372, 919, 932, 1642, 2433, 3008
BspQI GCTCTTC 2 cut(s) 877, 2913
BspT104I TTCGAA 1 cut(s) 3332
BspT107I GGYRCC 1 cut(s) 3318
BspTNI GGTCTC 5 cut(s) 503, 1205, 1351, 2760, 3173
BsrBI CCGCTC 1 cut(s) 3388
BsrDI GCAATG 2 cut(s) 672, 1374
BsrFI RCCGGY 1 cut(s) 494
BsrGI TGTACA 1 cut(s) 2145
BsrI ACTGG 5 cut(s) 613, 840, 1205, 2174, 2672
BssAI RCCGGY 1 cut(s) 494
BssECI CCNNGG 5 cut(s) 1056, 2575, 2750, 3168, 3183
BssNI GRCGYC 1 cut(s) 1276
BssT1I CCWWGG 4 cut(s) 1056, 2575, 3168, 3183
Bst2UI CCWGG 1 cut(s) 2751
Bst6I CTCTTC 4 cut(s) 877, 2468, 2913, 3435
BstACI GRCGYC 1 cut(s) 1276
BstAUI TGTACA 1 cut(s) 2145
BstBI TTCGAA 1 cut(s) 3332
BstC8I GCNNGC 6 cut(s) 642, 1547, 1680, 2233, 2303, 2467
BstDSI CCRYGG 1 cut(s) 1056
BstF5I GGATG 8 cut(s) 178, 342, 565, 711, 2632, 2812, 2884, 3136
BstHHI GCGC 2 cut(s) 2050, 3400
BstMAI GTCTC 8 cut(s) 127, 503, 863, 1205, 1351, 1720, 2760, 3173
BstMWI GCNNNNNNNGC 8 cut(s) 629, 1217, 1295, 2054, 2228, 3089, 3115, 3397
BstNI CCWGG 1 cut(s) 2751
BstNSI RCATGY 7 cut(s) 587, 729, 743, 755, 2091, 2591, 2906
BstPAI GACNNNNGTC 1 cut(s) 119
BstSCI CCNGG 1 cut(s) 2749
BstSFI CTRYAG 2 cut(s) 1710, 1787
BstSLI GKGCMC 4 cut(s) 751, 1234, 2106, 3323
BstV1I GCAGC 4 cut(s) 1273, 1280, 3044, 3328
BstV2I GAAGAC 3 cut(s) 206, 1443, 3218
BstX2I RGATCY 4 cut(s) 364, 924, 1858, 3488
BstXI CCANNNNNNTGG 2 cut(s) 1756, 2627
BstYI RGATCY 4 cut(s) 364, 924, 1858, 3488
BsuI GTATCC 1 cut(s) 2531
BsuRI GGCC 7 cut(s) 5, 839, 1607, 1678, 2057, 2391, 3083
BtgI CCRYGG 1 cut(s) 1056
BtrI CACGTC 1 cut(s) 535
BtsCI GGATG 8 cut(s) 178, 342, 565, 711, 2632, 2812, 2884, 3136
BtsI GCAGTG 2 cut(s) 684, 2217
BtsIMutI CAGTG 8 cut(s) 320, 684, 833, 1524, 1564, 2106, 2217, 2548
BveI ACCTGC 1 cut(s) 1303
Cac8I GCNNGC 6 cut(s) 642, 1547, 1680, 2233, 2303, 2467
CciI TCATGA 1 cut(s) 1144
CfoI GCGC 2 cut(s) 2050, 3400
Cfr10I RCCGGY 1 cut(s) 494
CseI GACGC 1 cut(s) 560
Csp6I GTAC 4 cut(s) 448, 1528, 2146, 2530
CviQI GTAC 4 cut(s) 448, 1528, 2146, 2530
DraI TTTAAA 1 cut(s) 2265
EaeI YGGCCR 5 cut(s) 837, 1605, 1676, 2055, 2389
Eam1104I CTCTTC 4 cut(s) 877, 2468, 2913, 3435
EarI CTCTTC 4 cut(s) 877, 2468, 2913, 3435
EciI GGCGGA 1 cut(s) 1189
Ecl136II GAGCTC 1 cut(s) 3118
Eco130I CCWWGG 4 cut(s) 1056, 2575, 3168, 3183
Eco24I GRGCYC 1 cut(s) 3120
Eco31I GGTCTC 5 cut(s) 503, 1205, 1351, 2760, 3173
Eco53kI GAGCTC 1 cut(s) 3118
Eco57I CTGAAG 6 cut(s) 1116, 1434, 1970, 2615, 2753, 3232
Eco88I CYCGRG 1 cut(s) 208
EcoICRI GAGCTC 1 cut(s) 3118
EcoRI GAATTC 1 cut(s) 875
EcoRII CCWGG 1 cut(s) 2749
EcoT14I CCWWGG 4 cut(s) 1056, 2575, 3168, 3183
EcoT22I ATGCAT 3 cut(s) 1587, 1953, 2089
EcoT38I GRGCYC 1 cut(s) 3120
ErhI CCWWGG 4 cut(s) 1056, 2575, 3168, 3183
FalI AAGNNNNNCTT 4 cut(s) 1123, 1155, 1373, 1405
FaqI GGGAC 3 cut(s) 208, 1821, 3186
FauI CCCGC 1 cut(s) 1897
FbaI TGATCA 1 cut(s) 306
FblI GTMKAC 2 cut(s) 102, 605
Fnu4HI GCNGC 5 cut(s) 1269, 1287, 3058, 3342, 3389
FokI GGATG 8 cut(s) 185, 329, 572, 718, 2639, 2819, 2891, 3143
FriOI GRGCYC 1 cut(s) 3120
Fsp4HI GCNGC 5 cut(s) 1269, 1287, 3058, 3342, 3389
FspBI CTAG 7 cut(s) 237, 431, 969, 1326, 1542, 1872, 2232
GlaI GCGC 2 cut(s) 2049, 3399
GluI GCNGC 5 cut(s) 1269, 1287, 3058, 3342, 3389
GsaI CCCAGC 1 cut(s) 851
GsuI CTGGAG 5 cut(s) 630, 1757, 2546, 2741, 2945
HaeIII GGCC 7 cut(s) 5, 839, 1607, 1678, 2057, 2391, 3083
HapII CCGG 1 cut(s) 495
HgaI GACGC 1 cut(s) 560
HhaI GCGC 2 cut(s) 2050, 3400
Hin1I GRCGYC 1 cut(s) 1276
Hin6I GCGC 2 cut(s) 2048, 3398
HinP1I GCGC 2 cut(s) 2048, 3398
HincII GTYRAC 3 cut(s) 2247, 2338, 2933
HindII GTYRAC 3 cut(s) 2247, 2338, 2933
HindIII AAGCTT 3 cut(s) 1536, 1742, 1920
HpaI GTTAAC 1 cut(s) 2247
HpaII CCGG 1 cut(s) 495
HphI GGTGA 6 cut(s) 252, 745, 1018, 2515, 2750, 3153
Hpy166II GTNNAC 8 cut(s) 103, 606, 749, 2247, 2281, 2338, 2480, 2933
Hpy8I GTNNAC 8 cut(s) 103, 606, 749, 2247, 2281, 2338, 2480, 2933
Hpy99I CGWCG 1 cut(s) 1278
HpyAV CCTTC 7 cut(s) 378, 418, 655, 1994, 2649, 3199, 3300
HpyCH4IV ACGT 7 cut(s) 164, 534, 1276, 1590, 2260, 2313, 2431
HpyF10VI GCNNNNNNNGC 8 cut(s) 629, 1217, 1295, 2054, 2228, 3089, 3115, 3397
HpySE526I ACGT 7 cut(s) 164, 534, 1276, 1590, 2260, 2313, 2431
Hsp92I GRCGYC 1 cut(s) 1276
HspAI GCGC 2 cut(s) 2048, 3398
Ksp22I TGATCA 1 cut(s) 306
KspAI GTTAAC 1 cut(s) 2247
LguI GCTCTTC 2 cut(s) 877, 2913
LmnI GCTCC 4 cut(s) 393, 3020, 3115, 3123
Lsp1109I GCAGC 4 cut(s) 1273, 1280, 3044, 3328
LweI GCATC 7 cut(s) 38, 1093, 1468, 1572, 2446, 2797, 2869
MaeI CTAG 7 cut(s) 237, 431, 969, 1326, 1542, 1872, 2232
MaeII ACGT 7 cut(s) 164, 534, 1276, 1590, 2260, 2313, 2431
MaeIII GTNAC 8 cut(s) 2095, 2165, 2185, 2256, 2309, 2452, 2503, 2742
MbiI CCGCTC 1 cut(s) 3388
MfeI CAATTG 3 cut(s) 1455, 3110, 3483
MflI RGATCY 4 cut(s) 364, 924, 1858, 3488
MhlI GDGCHC 6 cut(s) 14, 751, 1234, 2106, 3120, 3323
MlsI TGGCCA 3 cut(s) 1607, 1678, 2057
MluNI TGGCCA 3 cut(s) 1607, 1678, 2057
MlyI GAGTC 3 cut(s) 220, 2449, 2718
Mox20I TGGCCA 3 cut(s) 1607, 1678, 2057
Mph1103I ATGCAT 3 cut(s) 1587, 1953, 2089
MroXI GAANNNNTTC 2 cut(s) 1074, 3444
MscI TGGCCA 3 cut(s) 1607, 1678, 2057
MslI CAYNNNNRTG 3 cut(s) 744, 2561, 3306
Msp20I TGGCCA 3 cut(s) 1607, 1678, 2057
MspA1I CMGCKG 1 cut(s) 47
MspI CCGG 1 cut(s) 495
MspR9I CCNGG 1 cut(s) 2751
MssI GTTTAAAC 1 cut(s) 2265
MunI CAATTG 3 cut(s) 1455, 3110, 3483
Mva1269I GAATGC 4 cut(s) 2089, 2233, 2322, 3023
MvaI CCWGG 1 cut(s) 2751
MwoI GCNNNNNNNGC 8 cut(s) 629, 1217, 1295, 2054, 2228, 3089, 3115, 3397
NcoI CCATGG 1 cut(s) 1056
NheI GCTAGC 1 cut(s) 2231
NlaIV GGNNCC 4 cut(s) 366, 926, 2890, 3320
NmuCI GTSAC 5 cut(s) 2165, 2185, 2256, 2452, 2503
NsiI ATGCAT 3 cut(s) 1587, 1953, 2089
NspI RCATGY 7 cut(s) 587, 729, 743, 755, 2091, 2591, 2906
NspV TTCGAA 1 cut(s) 3332
PaeR7I CTCGAG 1 cut(s) 208
PagI TCATGA 1 cut(s) 1144
PciI ACATGT 5 cut(s) 583, 725, 739, 2587, 2902
PciSI GCTCTTC 2 cut(s) 877, 2913
PctI GAATGC 4 cut(s) 2089, 2233, 2322, 3023
PdmI GAANNNNTTC 2 cut(s) 1074, 3444
PkrI GCNGC 5 cut(s) 1270, 1288, 3059, 3343, 3390
PleI GAGTC 3 cut(s) 219, 2449, 2717
PmeI GTTTAAAC 1 cut(s) 2265
PpsI GAGTC 3 cut(s) 219, 2449, 2717
PscI ACATGT 5 cut(s) 583, 725, 739, 2587, 2902
PshAI GACNNNNGTC 1 cut(s) 119
PshBI ATTAAT 1 cut(s) 1686
Psp124BI GAGCTC 1 cut(s) 3120
Psp1406I AACGTT 2 cut(s) 164, 2313
Psp6I CCWGG 1 cut(s) 2749
PspFI CCCAGC 1 cut(s) 847
PspGI CCWGG 1 cut(s) 2749
PspN4I GGNNCC 4 cut(s) 366, 926, 2890, 3320
PspXI VCTCGAGB 1 cut(s) 208
PstI CTGCAG 1 cut(s) 1714
PsuI RGATCY 4 cut(s) 364, 924, 1858, 3488
PvuII CAGCTG 1 cut(s) 47
RsaI GTAC 4 cut(s) 449, 1529, 2147, 2531
RsaNI GTAC 4 cut(s) 448, 1528, 2146, 2530
RseI CAYNNNNRTG 3 cut(s) 744, 2561, 3306
SacI GAGCTC 1 cut(s) 3120
SapI GCTCTTC 2 cut(s) 877, 2913
SatI GCNGC 5 cut(s) 1269, 1287, 3058, 3342, 3389
SchI GAGTC 3 cut(s) 220, 2449, 2718
ScrFI CCNGG 1 cut(s) 2751
SduI GDGCHC 6 cut(s) 14, 751, 1234, 2106, 3120, 3323
SfaNI GCATC 7 cut(s) 38, 1093, 1468, 1572, 2446, 2797, 2869
SfcI CTRYAG 2 cut(s) 1710, 1787
Sfr274I CTCGAG 1 cut(s) 208
SfuI TTCGAA 1 cut(s) 3332
SlaI CTCGAG 1 cut(s) 208
SmiMI CAYNNNNRTG 3 cut(s) 744, 2561, 3306
SmlI CTYRAG 3 cut(s) 7, 208, 814
SmoI CTYRAG 3 cut(s) 7, 208, 814
SpeI ACTAGT 1 cut(s) 1871
SsiI CCGC 4 cut(s) 1174, 1890, 3388, 3424
SspI AATATT 2 cut(s) 823, 1690
SspMI CTAG 7 cut(s) 237, 431, 969, 1326, 1542, 1872, 2232
SstI GAGCTC 1 cut(s) 3120
StyD4I CCNGG 1 cut(s) 2749
StyI CCWWGG 4 cut(s) 1056, 2575, 3168, 3183
TaiI ACGT 7 cut(s) 167, 537, 1279, 1593, 2263, 2316, 2434
TaqI TCGA 7 cut(s) 209, 864, 1273, 1434, 1597, 2679, 3332
TatI WGTACW 2 cut(s) 2145, 2529
TauI GCSGC 1 cut(s) 3391
TscAI CASTG 8 cut(s) 320, 691, 840, 1524, 1564, 2106, 2224, 2548
TseFI GTSAC 5 cut(s) 2165, 2185, 2256, 2452, 2503
TseI GCWGC 4 cut(s) 1268, 1286, 3057, 3341
Tsp45I GTSAC 5 cut(s) 2165, 2185, 2256, 2452, 2503
TspRI CASTG 8 cut(s) 320, 691, 840, 1524, 1564, 2106, 2224, 2548
VneI GTGCAC 1 cut(s) 747
VspI ATTAAT 1 cut(s) 1686
XapI RAATTY 4 cut(s) 875, 1734, 2016, 2824
XbaI TCTAGA 2 cut(s) 236, 1325
XceI RCATGY 7 cut(s) 587, 729, 743, 755, 2091, 2591, 2906
XhoI CTCGAG 1 cut(s) 208
XmiI GTMKAC 2 cut(s) 102, 605
XmnI GAANNNNTTC 2 cut(s) 1074, 3444
XspI CTAG 7 cut(s) 237, 431, 969, 1326, 1542, 1872, 2232
ZraI GACGTC 1 cut(s) 1277
Zsp2I ATGCAT 3 cut(s) 1587, 1953, 2089
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.