Rroxscaffold_1G00003080

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000085
Physical Location & Seq
Forward (+)
3595699 .. 3616874
21176 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00003080.1

Sequence Viewer

Length: 3285 bp
ATGCAGTGGAGAGCTGCTTTAACAAAAGTGGGCAGTTTGTCTGGGTGGCATACACTGGATTACAAGTCTGAGAGAGATCTTGCCGAAGCCATTATGAAATCTGTGTGGAGTAAAGTAAAACAACGGGTTGTGGAAATCAACATGGGTGGTTTTGAAGTATTTAAAGCAACAAGACAAGCCATGGACGAGGTTATGAATGCGCTAAATGATGACAAGGTCACTGCTGTTGGGGTCTACGGCATGGGAGGCGTTGGAAAGACAATCATGGTGGAACATGTCTGTCAACTAGCTCAGAATAATGGCCTTTTTGATCATGTGATTATGGTTGTTGTTACCCAAAGCCCTGACTTCAGAAAAATTCAAAGCATGTTGGCAGATCAGTTGGGCGTCGGATTGGAGGAAACTACACTGAGAAAAATTCAAGGCACATTGGCAAATCAGTTTGGCACATTTGCAGATCAGTTCGGCATCAAATTGGAGGAAAGTACACAAAGTTCATGGGCAGCAAGACTGAGTAAGGAGATAATGAGGAGAAATAAGAACCTCATAATCTTAGACGACATTTGGGAGAGAATCGATTTGTCAAGAATAGGAATACCAAGCTACAAGGAACTTCAAAATTGCAATTCCAAAGTCCTACTCACCACAAGGATAAGGAATGTCTGTCATGTCATGAGGTGCCAAGAAAATATCACCCTCAGCACCCTATCAGAAGAAGATTCTTGGACCTTATTTGTGAGAAATGCAGGAAGGTTTTCTGAATCTACTAAATTCGAGGATGTAGCGAGGAAGGTGGCTAGAGAATGTGCTGGTCTCCCAATTGCGGTGATAAAAGTTGCAAGGGCACTCGGAAATAAATTCCTGGCGGAATGGAAAGAAGCAGCTCAACGACTAGAGAAGTCACAAACTGCCAACCCTGATCATGGAGAAGATGCATTCAGATGTATAAAGTTGAGCTATGATTACCTGAAAGAAGAAGATTCCAAGTCATGCTTCTTGCTTTGTTGCCTATTTCCAGAAGACTATGACATCCCAATAGAAGACTTGTTCAAGTATGCAATCGGGAAAGGATTGTTTCGACATGCCAAAACAATTGAAGAAGCCAGAGGAACAGCAGATTCAGTGGTCAGGTACCTGAAAGATTCTAGCTTGCTTTTGGATAGTGAAAAAAAGGGATGTGTAAGGATGCATGATGTCATCCGGGATACGGCCCTGAATATTGCAAAATCTGAAGATGGGCATGGGTTTTTGGTAAAAGCTGGCTGTGGTTTACAGGATTGGCCACGACAATTACATGAAGGTTACTCTGCAGTCTCACTAATGGAGAATGATATTAGCAAGCTACCCGAAGAAGAGTTGGTATGTCCAAAACTCAAGATTTTATTACTACAGGGGAATAATCATCTAGAAGAGATTCCAGAAGCTTTTTTCCAAAGTAGTAATGCACTGAGGGTCTTAGACCTTAGCCACACTAGTATTACATCACTTCCCCAATCATTCAGTCTCCTAACAAACCTTCAAGCTTTGTATTTTGAATCTTGCCCCATGATTGACTTTTCTAACCTGAGGACACTGAAGAAGCTTGAGATTCTGAGTATGAGAGGAAATGTGATCAGAGAATTGTCGAGAGAAATTGGGAATTTGACCAATCTAAGGATGTTGGACATTACCGGTGGACTTATTGGCAAAATTCCACCTAAAGTAATCTCAGAGTTGCATAGATTAGAAGAACTGTACATGGATTGTGGATTTTGGGAGTGGGGCAGTAAAGTTGAGGAAGAAGGAGAAGAAACTATTGGCTTTCATGAGGTAACTGGCTTATTATATTTAAACATTTTGAAGATTTGCATGTCTGATGCAAAATGCATCCCTAAAAAAGTTGAGTTCAATCCCAATTGGGTTTACTTTGATATTTGCATCAGTGGAGACAAAAGCACCAAAAACATAGTTAGAGACCTAAGCAATCAAATCCCTGAGTATGGTTGTAATTCAAGATCCTTGATTCTTGACACAACTATCAGTAACCTGCCAGATTGGTTTGTCAATGTGGTGACAAAGAACACAGAGAAGCTATACTACATAAAGAATCAGGATCTAAATAACATTCTTGTGGAATATCACCATGGAAGGTTACATAGTCTGAAGCATCTCTCTGTTATTGGTCCCAATGATGGCTTGAAAGAGTTGATGAACGGAATAACATGGGTTCCACGTACACCTGTGTTTGAGAACTTGGAAGAGTTGCATATAGAGAGAGTGGATTGCCTGGAAGAGTTATGCGTTGGTGAATTACCAGCTGGGTCTCTAGTCAATCTGAAGTTATTGAAGGTCCATTATTGTCCCAACTTGGGAAATGTACTTTTACCAACAACGTTGTTAAAGAGTTTGCCAAATTTGGAGAATCTAATCTGTAAGGATATACCTGGAATGAAATATGTGTTTGGATCTGAAGGGTCTGAGCAGCAATCAAGATTGAGAGAGATGACGTTCTTCAATTTATCTGCCGTAAGAAGCATATGTAATGGTCCTGCTCCACAAGCAATGTTCCAGAATCTTAAAAGTTTGTTCATCTACCGTTGCAACTTTCTAGGAGGTCTCTTCTCGTTTGATGCTGCTCAATGTCTATTTCAATTGAAAGACCTTTTTGTAGACAATTGTCCTTTACTGGAAAGACTAATTGAAGTAAGCAAAGAAACAGTGAGTGACAAGAAGACTGTTCTTCCAAAATTGAAGAACTTAGGTCTGGAGAAACTTCCTATGTTGTACAGTGGAAGTGGTACTATTGATATCGAGTGTCCTTCATTGAAACACTTGAGTGTGAAGGAGTGCCCCCAGTTTTCAGCCTTTGCTTCTGACTTCCAGAGCAGGAACCAAGTGCAACTCAACGATAAGTTATATTACTTTGGTCTAATACGCAAAAGTACTGAAAGAGAACTTGTTGAAGCCATTGCGGAATCTGTGTACTGTAAAGTAGAACCTATTGAAATCAATATGGGAGATTTTGAAGCATTTGAAGCAACAAAGCAAGCCACTATGGAAATTGAGCCTACAATGCCAAAGTCGACCAAAGATTTTGAAACAGTTGAAGCAACAACACAAGCCACCAATGGAAACTCCGAACCAGCTCAGTGGAAGCATGATGTGTTTTTGAGTTTTAGGGGTGAAGACACTCGCAAGGGTTTTCTATCCCATTTATACCATGAATTGCAAAACACGAAAGTAATTAGAACATTTAAGGATGATGAACAACTTGAGAAAGGGAAGGATATTTCTCAAGTCTACTGA

Protein Analysis

1094

Amino Acids

123.99

Weight (kDa)

5.71

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 59 - 250 5.5e-27 NB-ARC domain
LRR_8 PF13855 456 - 514 7.9e-11 Leucine rich repeat
LRR_14 PF23598 458 - 556 1.1e-10 Leucine-rich repeat region
LRR_14 PF23598 518 - 581 4.9e-07 Leucine-rich repeat region
LRR_RPS2 PF23247 746 - 886 3e-20 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 836 - 947 1.3e-13 Plant disease resistance protein RPS2-like, leucine-rich repeats
TIR PF01582 1045 - 1092 1.1e-10 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2034
Acc65I GGTACC 1 cut(s) 1131
AccB1I GGYRCC 2 cut(s) 678, 1131
AccI GTMKAC 4 cut(s) 234, 2649, 3062, 3279
AciI CCGC 3 cut(s) 824, 866, 2951
AclI AACGTT 1 cut(s) 2372
AclWI GGATC 3 cut(s) 1989, 2100, 2452
AcoI YGGCCR 1 cut(s) 1280
AcsI RAATTY 7 cut(s) 357, 417, 770, 857, 1639, 1689, 2392
AcuI CTGAAG 6 cut(s) 334, 1251, 1595, 2162, 2336, 2469
AcyI GRCGYC 1 cut(s) 387
AfaI GTAC 9 cut(s) 487, 1133, 1736, 2215, 2358, 2765, 2779, 2923, 2963
AfiI CCNNNNNNNGG 7 cut(s) 823, 923, 1207, 1653, 1979, 2171, 2348
AflIII ACRYGT 1 cut(s) 274
AgeI ACCGGT 1 cut(s) 1670
AhlI ACTAGT 1 cut(s) 1472
AjnI CCWGG 3 cut(s) 861, 2265, 2422
AjuI GAANNNNNNNTTGG 4 cut(s) 977, 1009, 1779, 1811
AleI CACNNNNGTG 1 cut(s) 2814
Alw26I GTCTC 7 cut(s) 818, 1318, 1508, 1920, 1947, 2307, 2600
AlwI GGATC 3 cut(s) 1989, 2100, 2452
AoxI GGCC 3 cut(s) 301, 1209, 1280
ApeKI GCWGC 5 cut(s) 14, 503, 881, 2461, 2612
ApoI RAATTY 7 cut(s) 357, 417, 770, 857, 1639, 1689, 2392
AsiGI ACCGGT 1 cut(s) 1670
Asp700I GAANNNNTTC 2 cut(s) 754, 1413
Asp718I GGTACC 1 cut(s) 1131
AspLEI GCGC 1 cut(s) 202
AspS9I GGNCC 5 cut(s) 726, 1210, 2162, 2329, 2525
AsuC2I CCSGG 1 cut(s) 1202
AsuHPI GGTGA 7 cut(s) 634, 685, 838, 2062, 2111, 2297, 3173
AvaII GGWCC 4 cut(s) 726, 2162, 2329, 2525
AxyI CCTNAGG 1 cut(s) 1565
BaeGI GKGCMC 2 cut(s) 847, 2831
BalI TGGCCA 1 cut(s) 1282
BanI GGYRCC 2 cut(s) 678, 1131
BarI GAAGNNNNNNTAC 2 cut(s) 2060, 2092
BbsI GAAGAC 4 cut(s) 1026, 1047, 2717, 3171
BbvCI CCTCAGC 1 cut(s) 698
BbvI GCAGC 4 cut(s) 515, 893, 2473, 2599
BccI CCATC 2 cut(s) 1229, 2165
BceAI ACGGC 3 cut(s) 253, 1224, 2489
BciT130I CCWGG 3 cut(s) 863, 2267, 2424
BciVI GTATCC 1 cut(s) 1198
BclI TGATCA 3 cut(s) 310, 919, 1611
BcnI CCSGG 1 cut(s) 1202
BcoDI GTCTC 7 cut(s) 818, 1318, 1508, 1920, 1947, 2307, 2600
BcuI ACTAGT 1 cut(s) 1472
BfaI CTAG 8 cut(s) 287, 798, 893, 1146, 1406, 1473, 2306, 2588
BfmI CTRYAG 2 cut(s) 1308, 1388
BfuAI ACCTGC 1 cut(s) 2034
BfuI GTATCC 1 cut(s) 1198
BglII AGATCT 1 cut(s) 76
BisI GCNGC 5 cut(s) 15, 504, 882, 2462, 2613
BlsI GCNGC 5 cut(s) 16, 505, 883, 2463, 2614
BmcAI AGTACT 1 cut(s) 2923
Bme1390I CCNGG 4 cut(s) 863, 1202, 2267, 2424
Bme18I GGWCC 4 cut(s) 726, 2162, 2329, 2525
BmgT120I GGNCC 5 cut(s) 726, 1210, 2162, 2329, 2525
BmiI GGNNCC 5 cut(s) 680, 1133, 2164, 2208, 2870
BmrFI CCNGG 4 cut(s) 863, 1202, 2267, 2424
BmrI ACTGGG 1 cut(s) 2827
BmsI GCATC 8 cut(s) 477, 922, 1176, 1846, 1875, 1926, 2155, 2599
BmuI ACTGGG 1 cut(s) 2827
BoxI GACNNNNGTC 1 cut(s) 2655
BpiI GAAGAC 4 cut(s) 1026, 1047, 2717, 3171
BpmI CTGGAG 1 cut(s) 2765
Bpu10I CCTNAGC 3 cut(s) 698, 1463, 1958
BpuEI CTTGAG 5 cut(s) 1358, 1604, 2833, 3258, 3272
BpuMI CCSGG 1 cut(s) 1202
Bsa29I ATCGAT 1 cut(s) 576
BsaAI YACGTR 1 cut(s) 2213
BsaHI GRCGYC 1 cut(s) 387
BsaI GGTCTC 4 cut(s) 818, 1947, 2307, 2600
BsaJI CCNNGG 2 cut(s) 180, 2122
BsaWI WCCGGW 1 cut(s) 1670
Bsc4I CCNNNNNNNGG 7 cut(s) 823, 923, 1207, 1653, 1979, 2171, 2348
Bse118I RCCGGY 1 cut(s) 1670
Bse1I ACTGG 4 cut(s) 60, 1819, 2670, 2833
Bse21I CCTNAGG 1 cut(s) 1565
Bse3DI GCAATG 2 cut(s) 2547, 2946
BseBI CCWGG 3 cut(s) 863, 2267, 2424
BseCI ATCGAT 1 cut(s) 576
BseDI CCNNGG 2 cut(s) 180, 2122
BseGI GGATG 8 cut(s) 784, 1029, 1181, 1191, 1197, 1662, 1866, 3244
BseLI CCNNNNNNNGG 7 cut(s) 823, 923, 1207, 1653, 1979, 2171, 2348
BseMI GCAATG 2 cut(s) 2547, 2946
BseNI ACTGG 4 cut(s) 60, 1819, 2670, 2833
BseRI GAGGAG 1 cut(s) 544
BseSI GKGCMC 2 cut(s) 847, 2831
BseXI GCAGC 4 cut(s) 515, 893, 2473, 2599
BseYI CCCAGC 1 cut(s) 2297
BshFI GGCC 3 cut(s) 303, 1211, 1282
BshNI GGYRCC 2 cut(s) 678, 1131
BshTI ACCGGT 1 cut(s) 1670
BshVI ATCGAT 1 cut(s) 576
BsiSI CCGG 2 cut(s) 1201, 1671
BslFI GGGAC 2 cut(s) 2148, 2325
BslI CCNNNNNNNGG 7 cut(s) 823, 923, 1207, 1653, 1979, 2171, 2348
BsmAI GTCTC 7 cut(s) 818, 1318, 1508, 1920, 1947, 2307, 2600
BsmFI GGGAC 2 cut(s) 2148, 2325
BsmI GAATGC 2 cut(s) 202, 935
BsnI GGCC 3 cut(s) 303, 1211, 1282
Bso31I GGTCTC 4 cut(s) 818, 1947, 2307, 2600
Bsp1286I GDGCHC 2 cut(s) 847, 2831
Bsp1407I TGTACA 2 cut(s) 1734, 2763
Bsp143I GATC 9 cut(s) 76, 310, 376, 457, 919, 1611, 1994, 2092, 2444
Bsp19I CCATGG 2 cut(s) 180, 2122
BspACI CCGC 3 cut(s) 824, 866, 2951
BspANI GGCC 3 cut(s) 303, 1211, 1282
BspDI ATCGAT 1 cut(s) 576
BspHI TCATGA 2 cut(s) 672, 1804
BspLI GGNNCC 5 cut(s) 680, 1133, 2164, 2208, 2870
BspMAI CTGCAG 1 cut(s) 1312
BspMI ACCTGC 1 cut(s) 2034
BspPI GGATC 3 cut(s) 1989, 2100, 2452
BspT107I GGYRCC 2 cut(s) 678, 1131
BspTNI GGTCTC 4 cut(s) 818, 1947, 2307, 2600
BsrDI GCAATG 2 cut(s) 2547, 2946
BsrFI RCCGGY 1 cut(s) 1670
BsrGI TGTACA 2 cut(s) 1734, 2763
BsrI ACTGG 4 cut(s) 60, 1819, 2670, 2833
BssAI RCCGGY 1 cut(s) 1670
BssECI CCNNGG 2 cut(s) 180, 2122
BssMI GATC 9 cut(s) 76, 310, 376, 457, 919, 1611, 1994, 2092, 2444
BssNI GRCGYC 1 cut(s) 387
BssT1I CCWWGG 2 cut(s) 180, 2122
Bst2UI CCWGG 3 cut(s) 863, 2267, 2424
Bst4CI ACNGT 7 cut(s) 1734, 2576, 2698, 2716, 2768, 2966, 3082
Bst6I CTCTTC 5 cut(s) 1347, 1404, 2232, 2265, 2603
BstACI GRCGYC 1 cut(s) 387
BstAUI TGTACA 2 cut(s) 1734, 2763
BstBAI YACGTR 1 cut(s) 2213
BstC8I GCNNGC 4 cut(s) 1151, 1261, 1340, 3027
BstDSI CCRYGG 2 cut(s) 180, 2122
BstF5I GGATG 8 cut(s) 784, 1029, 1181, 1191, 1197, 1662, 1866, 3244
BstHHI GCGC 1 cut(s) 202
BstKTI GATC 9 cut(s) 79, 313, 379, 460, 922, 1614, 1997, 2095, 2447
BstMAI GTCTC 7 cut(s) 818, 1318, 1508, 1920, 1947, 2307, 2600
BstMBI GATC 9 cut(s) 76, 310, 376, 457, 919, 1611, 1994, 2092, 2444
BstMWI GCNNNNNNNGC 4 cut(s) 246, 2537, 3014, 3052
BstNI CCWGG 3 cut(s) 863, 2267, 2424
BstNSI RCATGY 4 cut(s) 278, 370, 1085, 1852
BstPAI GACNNNNGTC 1 cut(s) 2655
BstSCI CCNGG 4 cut(s) 861, 1200, 2265, 2422
BstSFI CTRYAG 2 cut(s) 1308, 1388
BstSLI GKGCMC 2 cut(s) 847, 2831
BstV1I GCAGC 4 cut(s) 515, 893, 2473, 2599
BstV2I GAAGAC 4 cut(s) 1026, 1047, 2717, 3171
BstX2I RGATCY 4 cut(s) 76, 1994, 2092, 2444
BstXI CCANNNNNNTGG 1 cut(s) 3129
BstYI RGATCY 4 cut(s) 76, 1994, 2092, 2444
Bsu15I ATCGAT 1 cut(s) 576
Bsu36I CCTNAGG 1 cut(s) 1565
BsuI GTATCC 1 cut(s) 1198
BsuRI GGCC 3 cut(s) 303, 1211, 1282
BsuTUI ATCGAT 1 cut(s) 576
BtgI CCRYGG 2 cut(s) 180, 2122
BtsCI GGATG 8 cut(s) 784, 1029, 1181, 1191, 1197, 1662, 1866, 3244
BtsI GCAGTG 2 cut(s) 11, 219
BveI ACCTGC 1 cut(s) 2034
Cac8I GCNNGC 4 cut(s) 1151, 1261, 1340, 3027
CciI TCATGA 2 cut(s) 672, 1804
CfoI GCGC 1 cut(s) 202
Cfr10I RCCGGY 1 cut(s) 1670
Cfr13I GGNCC 5 cut(s) 726, 1210, 2162, 2329, 2525
ClaI ATCGAT 1 cut(s) 576
CseI GACGC 1 cut(s) 376
Csp6I GTAC 9 cut(s) 486, 1132, 1735, 2214, 2357, 2764, 2778, 2922, 2962
CspAI ACCGGT 1 cut(s) 1670
CspCI CAANNNNNGTGG 4 cut(s) 127, 162, 249, 284
CviQI GTAC 9 cut(s) 486, 1132, 1735, 2214, 2357, 2764, 2778, 2922, 2962
DpnI GATC 9 cut(s) 78, 312, 378, 459, 921, 1613, 1996, 2094, 2446
DpnII GATC 9 cut(s) 76, 310, 376, 457, 919, 1611, 1994, 2092, 2444
DraI TTTAAA 2 cut(s) 163, 1830
EaeI YGGCCR 1 cut(s) 1280
Eam1104I CTCTTC 5 cut(s) 1347, 1404, 2232, 2265, 2603
EarI CTCTTC 5 cut(s) 1347, 1404, 2232, 2265, 2603
EciI GGCGGA 1 cut(s) 881
Eco130I CCWWGG 2 cut(s) 180, 2122
Eco31I GGTCTC 4 cut(s) 818, 1947, 2307, 2600
Eco32I GATATC 1 cut(s) 2788
Eco47I GGWCC 4 cut(s) 726, 2162, 2329, 2525
Eco57I CTGAAG 6 cut(s) 334, 1251, 1595, 2162, 2336, 2469
Eco81I CCTNAGG 1 cut(s) 1565
EcoRII CCWGG 3 cut(s) 861, 2265, 2422
EcoRV GATATC 1 cut(s) 2788
EcoT14I CCWWGG 2 cut(s) 180, 2122
EcoT22I ATGCAT 3 cut(s) 937, 1191, 1868
ErhI CCWWGG 2 cut(s) 180, 2122
FalI AAGNNNNNCTT 2 cut(s) 977, 1009
FaqI GGGAC 2 cut(s) 2148, 2325
FauNDI CATATG 1 cut(s) 2516
FbaI TGATCA 3 cut(s) 310, 919, 1611
FblI GTMKAC 4 cut(s) 234, 2649, 3062, 3279
Fnu4HI GCNGC 5 cut(s) 15, 504, 882, 2462, 2613
FokI GGATG 8 cut(s) 791, 1016, 1184, 1188, 1198, 1669, 1853, 3251
Fsp4HI GCNGC 5 cut(s) 15, 504, 882, 2462, 2613
FspBI CTAG 8 cut(s) 287, 798, 893, 1146, 1406, 1473, 2306, 2588
GlaI GCGC 1 cut(s) 201
GluI GCNGC 5 cut(s) 15, 504, 882, 2462, 2613
GsaI CCCAGC 1 cut(s) 2301
GsuI CTGGAG 1 cut(s) 2765
HaeIII GGCC 3 cut(s) 303, 1211, 1282
HapII CCGG 2 cut(s) 1201, 1671
HgaI GACGC 1 cut(s) 376
HhaI GCGC 1 cut(s) 202
Hin1I GRCGYC 1 cut(s) 387
Hin6I GCGC 1 cut(s) 200
HinP1I GCGC 1 cut(s) 200
HincII GTYRAC 2 cut(s) 284, 3063
HindII GTYRAC 2 cut(s) 284, 3063
HindIII AAGCTT 3 cut(s) 1422, 1521, 1580
HpaII CCGG 2 cut(s) 1201, 1671
HphI GGTGA 7 cut(s) 634, 685, 838, 2062, 2111, 2297, 3173
Hpy99I CGWCG 1 cut(s) 392
HpyCH4III ACNGT 7 cut(s) 1734, 2576, 2698, 2716, 2768, 2966, 3082
HpyCH4IV ACGT 3 cut(s) 2212, 2372, 2486
HpyF10VI GCNNNNNNNGC 4 cut(s) 246, 2537, 3014, 3052
HpySE526I ACGT 3 cut(s) 2212, 2372, 2486
Hsp92I GRCGYC 1 cut(s) 387
HspAI GCGC 1 cut(s) 200
KpnI GGTACC 1 cut(s) 1135
Ksp22I TGATCA 3 cut(s) 310, 919, 1611
Kzo9I GATC 9 cut(s) 76, 310, 376, 457, 919, 1611, 1994, 2092, 2444
LmnI GCTCC 1 cut(s) 2536
Lsp1109I GCAGC 4 cut(s) 515, 893, 2473, 2599
LweI GCATC 8 cut(s) 477, 922, 1176, 1846, 1875, 1926, 2155, 2599
MaeI CTAG 8 cut(s) 287, 798, 893, 1146, 1406, 1473, 2306, 2588
MaeII ACGT 3 cut(s) 2212, 2372, 2486
MaeIII GTNAC 9 cut(s) 217, 331, 900, 1301, 1810, 2021, 2050, 2130, 2702
MalI GATC 9 cut(s) 78, 312, 378, 459, 921, 1613, 1996, 2094, 2446
MboI GATC 9 cut(s) 76, 310, 376, 457, 919, 1611, 1994, 2092, 2444
MfeI CAATTG 5 cut(s) 819, 1092, 1894, 2630, 2653
MflI RGATCY 4 cut(s) 76, 1994, 2092, 2444
MhlI GDGCHC 2 cut(s) 847, 2831
MlsI TGGCCA 1 cut(s) 1282
MluNI TGGCCA 1 cut(s) 1282
MmeI TCCRAC 3 cut(s) 232, 370, 1641
Mox20I TGGCCA 1 cut(s) 1282
Mph1103I ATGCAT 3 cut(s) 937, 1191, 1868
MroXI GAANNNNTTC 2 cut(s) 754, 1413
MscI TGGCCA 1 cut(s) 1282
MseI TTAA 6 cut(s) 20, 162, 1829, 2378, 2556, 3234
MslI CAYNNNNRTG 3 cut(s) 940, 2108, 2814
Msp20I TGGCCA 1 cut(s) 1282
MspA1I CMGCKG 1 cut(s) 2297
MspI CCGG 2 cut(s) 1201, 1671
MspR9I CCNGG 4 cut(s) 863, 1202, 2267, 2424
MunI CAATTG 5 cut(s) 819, 1092, 1894, 2630, 2653
Mva1269I GAATGC 2 cut(s) 202, 935
MvaI CCWGG 3 cut(s) 863, 2267, 2424
MwoI GCNNNNNNNGC 4 cut(s) 246, 2537, 3014, 3052
NciI CCSGG 1 cut(s) 1202
NcoI CCATGG 2 cut(s) 180, 2122
NdeI CATATG 1 cut(s) 2516
NdeII GATC 9 cut(s) 76, 310, 376, 457, 919, 1611, 1994, 2092, 2444
NlaIV GGNNCC 5 cut(s) 680, 1133, 2164, 2208, 2870
NmuCI GTSAC 4 cut(s) 217, 900, 2050, 2702
NsiI ATGCAT 3 cut(s) 937, 1191, 1868
NspI RCATGY 4 cut(s) 278, 370, 1085, 1852
OliI CACNNNNGTG 1 cut(s) 2814
PagI TCATGA 2 cut(s) 672, 1804
PciI ACATGT 1 cut(s) 274
PctI GAATGC 2 cut(s) 202, 935
PdmI GAANNNNTTC 2 cut(s) 754, 1413
PflFI GACNNNGTC 1 cut(s) 215
PfoI TCCNGGA 1 cut(s) 1200
PinAI ACCGGT 1 cut(s) 1670
PkrI GCNGC 5 cut(s) 16, 505, 883, 2463, 2614
Ppu21I YACGTR 1 cut(s) 2213
PscI ACATGT 1 cut(s) 274
PshAI GACNNNNGTC 1 cut(s) 2655
Psp1406I AACGTT 1 cut(s) 2372
Psp6I CCWGG 3 cut(s) 861, 2265, 2422
PspFI CCCAGC 1 cut(s) 2297
PspGI CCWGG 3 cut(s) 861, 2265, 2422
PspN4I GGNNCC 5 cut(s) 680, 1133, 2164, 2208, 2870
PspPI GGNCC 5 cut(s) 726, 1210, 2162, 2329, 2525
PsrI GAACNNNNNNTAC 2 cut(s) 478, 510
PstI CTGCAG 1 cut(s) 1312
PsuI RGATCY 4 cut(s) 76, 1994, 2092, 2444
PsyI GACNNNGTC 1 cut(s) 215
PvuII CAGCTG 1 cut(s) 2297
RsaI GTAC 9 cut(s) 487, 1133, 1736, 2215, 2358, 2765, 2779, 2923, 2963
RsaNI GTAC 9 cut(s) 486, 1132, 1735, 2214, 2357, 2764, 2778, 2922, 2962
RseI CAYNNNNRTG 3 cut(s) 940, 2108, 2814
SalI GTCGAC 1 cut(s) 3061
SaqAI TTAA 6 cut(s) 20, 162, 1829, 2378, 2556, 3234
SatI GCNGC 5 cut(s) 15, 504, 882, 2462, 2613
Sau3AI GATC 9 cut(s) 76, 310, 376, 457, 919, 1611, 1994, 2092, 2444
Sau96I GGNCC 5 cut(s) 726, 1210, 2162, 2329, 2525
ScaI AGTACT 1 cut(s) 2923
ScrFI CCNGG 4 cut(s) 863, 1202, 2267, 2424
SduI GDGCHC 2 cut(s) 847, 2831
SfaNI GCATC 8 cut(s) 477, 922, 1176, 1846, 1875, 1926, 2155, 2599
SfcI CTRYAG 2 cut(s) 1308, 1388
SinI GGWCC 4 cut(s) 726, 2162, 2329, 2525
SmiMI CAYNNNNRTG 3 cut(s) 940, 2108, 2814
SmlI CTYRAG 5 cut(s) 1373, 1583, 2812, 3251, 3273
SmoI CTYRAG 5 cut(s) 1373, 1583, 2812, 3251, 3273
SpeI ACTAGT 1 cut(s) 1472
SsiI CCGC 3 cut(s) 824, 866, 2951
SspI AATATT 1 cut(s) 1219
SspMI CTAG 8 cut(s) 287, 798, 893, 1146, 1406, 1473, 2306, 2588
StyD4I CCNGG 4 cut(s) 861, 1200, 2265, 2422
StyI CCWWGG 2 cut(s) 180, 2122
TaaI ACNGT 7 cut(s) 1734, 2576, 2698, 2716, 2768, 2966, 3082
TaiI ACGT 3 cut(s) 2215, 2375, 2489
TaqI TCGA 6 cut(s) 576, 774, 1078, 1625, 2790, 3062
TatI WGTACW 6 cut(s) 485, 1734, 2356, 2763, 2921, 2961
Tru1I TTAA 6 cut(s) 20, 162, 1829, 2378, 2556, 3234
Tru9I TTAA 6 cut(s) 20, 162, 1829, 2378, 2556, 3234
TseFI GTSAC 4 cut(s) 217, 900, 2050, 2702
TseI GCWGC 5 cut(s) 14, 503, 881, 2461, 2612
Tsp45I GTSAC 4 cut(s) 217, 900, 2050, 2702
TspGWI ACGGA 1 cut(s) 2208
Tth111I GACNNNGTC 1 cut(s) 215
VpaK11BI GGWCC 4 cut(s) 726, 2162, 2329, 2525
XapI RAATTY 7 cut(s) 357, 417, 770, 857, 1639, 1689, 2392
XbaI TCTAGA 1 cut(s) 1405
XceI RCATGY 4 cut(s) 278, 370, 1085, 1852
XmiI GTMKAC 4 cut(s) 234, 2649, 3062, 3279
XmnI GAANNNNTTC 2 cut(s) 754, 1413
XspI CTAG 8 cut(s) 287, 798, 893, 1146, 1406, 1473, 2306, 2588
ZrmI AGTACT 1 cut(s) 2923
Zsp2I ATGCAT 3 cut(s) 937, 1191, 1868
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.