RchiOBHm_Chr3g0473481

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000085
Physical Location & Seq
Forward (+)
19320101 .. 19325505
5405 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43914

Sequence Viewer

Length: 3387 bp
ATGGAAGAAGACAACAATAGAATTCTGCCACTTTTTTATTATGTGGATCCTACTGATGTACGATATCAGAAGAGGAGTTTCGGAGATGCTTTCACTAAGCATGAAAACTCTGGGCGACACAGATCAGAGAAGGTGCAGCAGTGGAGAGATTCTTTAAAAAAAGTGGCAAATTTCTCTGGATGGCATATACTGAATCATAAATCTGAAAGAGAACTTTTGGATGCCATTGAGGAATCTGTGTGCAATAAATTACGACCTACTGAACTTGAGTTTAAATTGTCCATCGGAGGTTTTGAAGCATTTGCAGCAACAAAACAAGCCATTGATAAGGTCATGAATGCTCTAAAAGATGATGAGGCCACTACCATTGGAGTCTACGGAATGGGGGGCGTTGGAAAGACAGCAGTGGTGAAATATGTTGGAGCACAAGCCCAAAAAAGTAGGCTTTTTAATCAAGTGATTATGGCTGTCGTATCCCAAAACCCCGACTTGATAAAAATTCAAGAGACATTTGCAGAACTGCTGGGCTTTAAATTGGAGGAGAAGACAGAAATTGGAAGATCCATTAAATTGAAGGAGAAGATAATGAGAGGAACCGGGATCCTCATAATCTTGGATGACATTTGGAAGAGAATAGACTTTTCAAGCATAGGAATTCCAAGCCACAACGAACTTCAAAGATGCAATTCCAAAGTCCTACTGACCACCAGAAGATTGACTGTTTGTCAGATCATGGATAGCCATGCAAAAATTCATCTCAATATCTTATCAAAAGAAGATTCTTGGAGCTTATTTTTGAAGGAAGCAAGGAAATCTTTTGACAAATCTTCCAATTTCTATGTTGTAGCGAGTGAGGTGGCAAGAGAATGCGCCGGTCTACCAGTTGCCTTGATAGCAGTTGCGAGGGCCCTTCGAGATGAAGGTTTGGACGGATGGAAAGAAGCTGCTCGACGACTGAAAGCATCTCAACCTCCCTTCCCTGAAGATGAGGGAGATGTTTTCAAATGCATAAAGTTGAGTTATGATTACTTGAAATCTGATGATTCCAAATCATGCTTCTTGCTTTGCTGCCTGTTCCCAGAAGATTATGATATCCCAATTGAATACTTGTTCAAGTATGGAATTGGGAAAGGAATGTTTCAAGATTCAAACTTGCTACAGGCCCGAGCCACAACATATTTAGTGGTGAAGGCCCTTAAAGATTCTAGTTTGCTTTTGGACGCTAGAAATGACAGATGTGTAAAGATGCATGATGTCATTCGGGATATGGCAATCTCAATTTTGTTATCTGAAGGACAGCCGGTCTTGGTGAAAGCTGGCTGTGAATTGAAGAATTGGCCACCGATTGATGCACATAAAGCCTACTCTGCAATCTCACTAATGGAGAACAAAATTTGCAAGCTACCCCAAGAGTTGGTATGTCCAAATCTCCAGATTTTATTACTGCAACACAATGCTTCATTAAATGATATCCCAAACTCTTTCTTCCAATGTCAGAAAGAATTAGGAGTCTTAGATCTTAGCGGCACTGGTATTTCATTACTACCGCACTCAATCAGTTTCCTAACCAACCTTCAAGCTTTGTATTTAGATTATTGCAAGAACATAATTGACATTTCTGTAATCGGAAAACTTAACAAGCTTGAGATTCTTAGTATGAGAGAAAATACTCAGAGGGAATTGCCAAGGGAAATAGGACGATTGACCAATCTAAGGATGTTGGATATCAGTAGTGATTCTATACCCCAATCCCCATATGGATATAATCGCACAATTCCATCTAAAGTGATATCAAAGTTGCATAAATTAGAAGAACTGTACATGCAATGTGTATTTTGGGACTGGGGGAGTAAAATTGTTGGAGATGGAGAAGAAACTAATATTGGCTTTGATGAATTGGCTGGTTTATCATATTTAAGCATTTTGAAAGTTTGCATATCTGATGCAAATTGCATCCCTAAAACTGTTGATGTCGAACCGAATTGGGTTTACTTTGATATTAGAATTTCCAGCAGACATATGAGGGACAGTGTGAACTTACAATCTGGTCATAATTGTAGAACCTTAAGTCTTGATAGCACTGACACAACCATAGGAACCTTTCCGGACTGGTTTGTCAACGCTGCGGTAAAGAATACTGAGAGGCTATGTTATGAAGAATGCCGAGGGTTAAGTAACATTCTTGTTGAATATGACCGTGGTAGGTTACATGGACTGAAAGTTCTCTCTGTAAGAGGTTGCTGTGAGAACTTGAAAGAGTTGATGAACGCAAAAACATGCGTTTCTGATAAGCCTGTGTTTGAGAACTTGGAAGAGTTGTATTTGAAAGACATGGATGACCTGAAACAGTTATGTGTTGGTGAGTTACCACCTGGATCTCTCTGCAGTCTCAAATTATTGAAGGTCTGTAAGTGTCCTAACTTGGAGAATGTGCTGTTGCCATCAGAATTGTTGCAGAAACTACCAAATCTGGAAGAACTATTCTGTGAGGCAAGTGGAGTGGAATATGTGTTTGGATGTGAAGGATTTGAGCCGGATCAAACAAATCTGAGAAAGATGGAGTTGATGGATCTAGACGTAGTAAGAAGCATATGTAATGGTCCTGCTCCAAGTGGAATGTTCCAAGCTCTGATGGAATTGGTCATTATGTATTGCAATTTCCAGGGAAGCCTGTTCACATTTGATGTCTCTCAGTGTCTTTTTCAATTGGAAACCCTTACTGTATTCGAATGCCCTGTCTTGGAAAGAGTAATCGAAGCAAGGAGGGAAACATTGAAGAACAAGAAGACCATTCTTCCAAAACTGAAGAACTTATGTTTGATAGATCTTCCAATGTTGTACAAGGGAGGTGCTACTATTGATTTTGACTGTCCTTCATTGGAAAACTTTTATATGGAGGATTGCCCTCACTTGTCATTTCCGTCCTCTGCTTCTGACTACTTCCACAGCAGGAACCAAGTCAATGGTGATAAGCCTAAAAACTGTATCCCACTCCTCGATATAAAGTATTCTTCGAAAAGTGGAAAGAAGGCAACGAAAGTATGCCGCGGAACTCCTCGACATGGACAGACTGCTAGAGCACTTGCAAAACAGGCAAACGGAACTGAGGATAATGCTAGAAAATCAAGACAGCCCGGGGGTGGAAGAGGTAGAGTGAGGGACTTCTTTGCTTCACACGGAGAGAGAGAGAGAGGGGGGGGAACGAGTTCTGGTTGGAATTCAAACAGGGAACTGAAGCTTCCAGAATCGACGACTCGCGCCAACCACTCCGGATTTTTCCTCAGCCATCTGAATTTCGGGGATTTCATCGTTCTCGAACTTCCGGCGAAGCCCCCGCGCCACAGAAGCCGAAGAAAGAACCTACCGATGATGAAAAGAGAAGGATGA

Protein Analysis

1128

Amino Acids

127.34

Weight (kDa)

7.58

Isoelectric Point (pI)

41.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 2 - 88 3.7e-13 TIR domain
NB-ARC PF00931 105 - 270 7e-29 NB-ARC domain
LRR_8 PF13855 475 - 533 2.2e-07 Leucine rich repeat
LRR_14 PF23598 500 - 692 9.5e-14 Leucine-rich repeat region
LRR_RPS2 PF23247 710 - 827 2.4e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 770 - 911 9.2e-18 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 851 - 975 6.9e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2114
AccB7I CCANNNNNTGG 1 cut(s) 1414
AccI GTMKAC 2 cut(s) 375, 877
AccII CGCG 3 cut(s) 3048, 3258, 3337
AccIII TCCGGA 2 cut(s) 2104, 3269
AciI CCGC 6 cut(s) 1524, 1547, 2126, 3046, 3048, 3335
AclWI GGATC 8 cut(s) 41, 54, 555, 595, 608, 2383, 2544, 2577
AcoI YGGCCR 1 cut(s) 1337
AcsI RAATTY 9 cut(s) 21, 169, 498, 654, 750, 1392, 2004, 3217, 3292
AcuI CTGAAG 4 cut(s) 1002, 1311, 2825, 3254
AfaI GTAC 3 cut(s) 60, 1820, 2840
AfiI CCNNNNNNNGG 5 cut(s) 1414, 1713, 2740, 3062, 3140
AflII CTTAAG 1 cut(s) 2065
AhdI GACNNNNNGTC 2 cut(s) 723, 1301
AjnI CCWGG 2 cut(s) 2371, 2661
AjuI GAANNNNNNNTTGG 6 cut(s) 1040, 1072, 1866, 1898, 2496, 2528
AloI GAACNNNNNNTCC 2 cut(s) 2205, 2237
AluBI AGCT 8 cut(s) 789, 944, 1316, 1402, 1580, 1642, 2627, 3238
AluI AGCT 8 cut(s) 789, 944, 1316, 1402, 1580, 1642, 2627, 3238
Alw21I GWGCWC 2 cut(s) 427, 3082
Alw26I GTCTC 3 cut(s) 500, 2393, 2692
AlwI GGATC 8 cut(s) 41, 54, 555, 595, 608, 2383, 2544, 2577
Ama87I CYCGRG 2 cut(s) 1164, 3134
Aor13HI TCCGGA 2 cut(s) 2104, 3269
AoxI GGCC 5 cut(s) 357, 906, 1161, 1191, 1337
ApaI GGGCCC 1 cut(s) 910
ApeKI GCWGC 5 cut(s) 136, 305, 944, 1068, 2123
ApoI RAATTY 9 cut(s) 21, 169, 498, 654, 750, 1392, 2004, 3217, 3292
ArsI GACNNNNNNTTYG 2 cut(s) 2721, 2753
Asp700I GAANNNNTTC 1 cut(s) 3205
AspLEI GCGC 3 cut(s) 872, 3260, 3339
AspS9I GGNCC 5 cut(s) 906, 907, 1162, 1192, 2600
AsuC2I CCSGG 3 cut(s) 598, 3135, 3136
AsuHPI GGTGA 5 cut(s) 421, 1198, 1321, 2372, 2978
AsuII TTCGAA 2 cut(s) 2727, 3014
AvaI CYCGRG 2 cut(s) 1164, 3134
AvaII GGWCC 1 cut(s) 2600
BaeGI GKGCMC 1 cut(s) 910
BalI TGGCCA 1 cut(s) 1339
BamHI GGATCC 2 cut(s) 46, 600
BanII GRGCYC 1 cut(s) 910
BbsI GAAGAC 3 cut(s) 15, 551, 2792
Bbv12I GWGCWC 2 cut(s) 427, 3082
BbvCI CCTCAGC 1 cut(s) 3281
BbvI GCAGC 5 cut(s) 148, 317, 931, 1055, 2110
BciT130I CCWGG 2 cut(s) 2373, 2663
BciVI GTATCC 2 cut(s) 484, 2996
BcnI CCSGG 3 cut(s) 598, 3135, 3136
BcoDI GTCTC 3 cut(s) 500, 2393, 2692
BfaI CTAG 5 cut(s) 1206, 1224, 2573, 3075, 3117
BfmI CTRYAG 2 cut(s) 1157, 2383
BfrI CTTAAG 1 cut(s) 2065
BfuI GTATCC 2 cut(s) 484, 2996
BglII AGATCT 2 cut(s) 1516, 2824
BisI GCNGC 7 cut(s) 137, 306, 945, 1069, 1525, 2124, 3046
BlsI GCNGC 7 cut(s) 138, 307, 946, 1070, 1526, 2125, 3047
Bme1390I CCNGG 5 cut(s) 598, 2373, 2663, 3135, 3136
Bme18I GGWCC 1 cut(s) 2600
BmeRI GACNNNNNGTC 2 cut(s) 723, 1301
BmeT110I CYCGRG 2 cut(s) 1164, 3134
BmgT120I GGNCC 5 cut(s) 906, 907, 1162, 1192, 2600
BmiI GGNNCC 6 cut(s) 48, 595, 602, 908, 2098, 2954
BmrFI CCNGG 5 cut(s) 598, 2373, 2663, 3135, 3136
BmrI ACTGGG 1 cut(s) 1852
BmsI GCATC 8 cut(s) 76, 211, 671, 971, 1236, 1339, 1933, 1962
BmuI ACTGGG 1 cut(s) 1852
BpiI GAAGAC 3 cut(s) 15, 551, 2792
BpmI CTGGAG 1 cut(s) 1415
Bpu10I CCTNAGC 1 cut(s) 3281
Bpu14I TTCGAA 2 cut(s) 2727, 3014
BpuEI CTTGAG 2 cut(s) 287, 1664
BpuMI CCSGG 3 cut(s) 598, 3135, 3136
BsaJI CCNNGG 7 cut(s) 1685, 2164, 2197, 2662, 3046, 3134, 3135
BsaWI WCCGGW 2 cut(s) 2104, 3269
Bsc4I CCNNNNNNNGG 5 cut(s) 1414, 1713, 2740, 3062, 3140
Bse118I RCCGGY 2 cut(s) 872, 1300
Bse1I ACTGG 4 cut(s) 881, 1534, 1847, 2114
Bse3DI GCAATG 1 cut(s) 1832
BseAI TCCGGA 2 cut(s) 2104, 3269
BseBI CCWGG 2 cut(s) 2373, 2663
BseDI CCNNGG 7 cut(s) 1685, 2164, 2197, 2662, 3046, 3134, 3135
BseGI GGATG 8 cut(s) 185, 226, 622, 938, 1722, 1953, 2341, 2522
BseLI CCNNNNNNNGG 5 cut(s) 1414, 1713, 2740, 3062, 3140
BseMI GCAATG 1 cut(s) 1832
BseMII CTCAG 6 cut(s) 1685, 2130, 2540, 2705, 3096, 3295
BseNI ACTGG 4 cut(s) 881, 1534, 1847, 2114
BseRI GAGGAG 4 cut(s) 88, 554, 2984, 3045
BseSI GKGCMC 1 cut(s) 910
BseXI GCAGC 5 cut(s) 148, 317, 931, 1055, 2110
BseYI CCCAGC 1 cut(s) 523
BsgI GTGCAG 1 cut(s) 155
Bsh1236I CGCG 3 cut(s) 3048, 3258, 3337
BshFI GGCC 5 cut(s) 359, 908, 1163, 1193, 1339
BsiHKAI GWGCWC 2 cut(s) 427, 3082
BsiHKCI CYCGRG 2 cut(s) 1164, 3134
BsiSI CCGG 8 cut(s) 597, 873, 1301, 2105, 2534, 3135, 3270, 3323
BslFI GGGAC 3 cut(s) 1853, 2039, 3173
BslI CCNNNNNNNGG 5 cut(s) 1414, 1713, 2740, 3062, 3140
BsmAI GTCTC 3 cut(s) 500, 2393, 2692
BsmFI GGGAC 3 cut(s) 1853, 2039, 3173
BsmI GAATGC 4 cut(s) 343, 872, 2165, 2735
BsnI GGCC 5 cut(s) 359, 908, 1163, 1193, 1339
BsoBI CYCGRG 2 cut(s) 1164, 3134
Bsp119I TTCGAA 2 cut(s) 2727, 3014
Bsp120I GGGCCC 1 cut(s) 906
Bsp1286I GDGCHC 3 cut(s) 427, 910, 3082
Bsp13I TCCGGA 2 cut(s) 2104, 3269
Bsp1407I TGTACA 2 cut(s) 1818, 2838
BspACI CCGC 6 cut(s) 1524, 1547, 2126, 3046, 3048, 3335
BspANI GGCC 5 cut(s) 359, 908, 1163, 1193, 1339
BspCNI CTCAG 6 cut(s) 1684, 2131, 2541, 2704, 3097, 3294
BspEI TCCGGA 2 cut(s) 2104, 3269
BspFNI CGCG 3 cut(s) 3048, 3258, 3337
BspHI TCATGA 1 cut(s) 333
BspLI GGNNCC 6 cut(s) 48, 595, 602, 908, 2098, 2954
BspMAI CTGCAG 1 cut(s) 2387
BspPI GGATC 8 cut(s) 41, 54, 555, 595, 608, 2383, 2544, 2577
BspT104I TTCGAA 2 cut(s) 2727, 3014
BspTI CTTAAG 1 cut(s) 2065
BsrDI GCAATG 1 cut(s) 1832
BsrFI RCCGGY 2 cut(s) 872, 1300
BsrGI TGTACA 2 cut(s) 1818, 2838
BsrI ACTGG 4 cut(s) 881, 1534, 1847, 2114
BssAI RCCGGY 2 cut(s) 872, 1300
BssECI CCNNGG 7 cut(s) 1685, 2164, 2197, 2662, 3046, 3134, 3135
BssT1I CCWWGG 1 cut(s) 1685
Bst2UI CCWGG 2 cut(s) 2373, 2663
Bst4CI ACNGT 9 cut(s) 721, 1818, 1966, 2030, 2198, 2349, 2722, 2870, 2984
Bst6I CTCTTC 4 cut(s) 65, 623, 2307, 3139
BstAFI CTTAAG 1 cut(s) 2065
BstAUI TGTACA 2 cut(s) 1818, 2838
BstBI TTCGAA 2 cut(s) 2727, 3014
BstC8I GCNNGC 2 cut(s) 1318, 1400
BstDSI CCRYGG 2 cut(s) 2197, 3046
BstF5I GGATG 8 cut(s) 185, 226, 622, 938, 1722, 1953, 2341, 2522
BstFNI CGCG 3 cut(s) 3048, 3258, 3337
BstHHI GCGC 3 cut(s) 872, 3260, 3339
BstMAI GTCTC 3 cut(s) 500, 2393, 2692
BstMWI GCNNNNNNNGC 6 cut(s) 305, 893, 1358, 1367, 3092, 3345
BstNI CCWGG 2 cut(s) 2373, 2663
BstNSI RCATGY 2 cut(s) 1825, 2280
BstSCI CCNGG 5 cut(s) 596, 2371, 2661, 3133, 3134
BstSFI CTRYAG 2 cut(s) 1157, 2383
BstSLI GKGCMC 1 cut(s) 910
BstUI CGCG 3 cut(s) 3048, 3258, 3337
BstV1I GCAGC 5 cut(s) 148, 317, 931, 1055, 2110
BstV2I GAAGAC 3 cut(s) 15, 551, 2792
BstX2I RGATCY 7 cut(s) 46, 560, 600, 1516, 2375, 2569, 2824
BstXI CCANNNNNNTGG 1 cut(s) 2963
BstYI RGATCY 7 cut(s) 46, 560, 600, 1516, 2375, 2569, 2824
BsuI GTATCC 2 cut(s) 484, 2996
BsuRI GGCC 5 cut(s) 359, 908, 1163, 1193, 1339
BtgI CCRYGG 2 cut(s) 2197, 3046
BtsCI GGATG 8 cut(s) 185, 226, 622, 938, 1722, 1953, 2341, 2522
BtsI GCAGTG 2 cut(s) 146, 411
BtsIMutI CAGTG 6 cut(s) 146, 411, 1527, 2035, 2079, 2699
Cac8I GCNNGC 2 cut(s) 1318, 1400
CciI TCATGA 1 cut(s) 333
CfoI GCGC 3 cut(s) 872, 3260, 3339
Cfr10I RCCGGY 2 cut(s) 872, 1300
Cfr13I GGNCC 5 cut(s) 906, 907, 1162, 1192, 2600
Cfr42I CCGCGG 1 cut(s) 3049
Cfr9I CCCGGG 1 cut(s) 3134
CseI GACGC 1 cut(s) 1229
Csp6I GTAC 3 cut(s) 59, 1819, 2839
CspCI CAANNNNNGTGG 4 cut(s) 349, 384, 2481, 2516
CviQI GTAC 3 cut(s) 59, 1819, 2839
DraI TTTAAA 3 cut(s) 156, 274, 532
DrdI GACNNNNNNGTC 1 cut(s) 2114
DriI GACNNNNNGTC 2 cut(s) 723, 1301
DseDI GACNNNNNNGTC 1 cut(s) 2114
EaeI YGGCCR 1 cut(s) 1337
Eam1104I CTCTTC 4 cut(s) 65, 623, 2307, 3139
Eam1105I GACNNNNNGTC 2 cut(s) 723, 1301
EarI CTCTTC 4 cut(s) 65, 623, 2307, 3139
Eco130I CCWWGG 1 cut(s) 1685
Eco24I GRGCYC 1 cut(s) 910
Eco32I GATATC 5 cut(s) 65, 1093, 1471, 1726, 1791
Eco47I GGWCC 1 cut(s) 2600
Eco57I CTGAAG 4 cut(s) 1002, 1311, 2825, 3254
Eco88I CYCGRG 2 cut(s) 1164, 3134
EcoO109I RGGNCCY 3 cut(s) 906, 907, 1192
EcoRI GAATTC 3 cut(s) 21, 654, 3217
EcoRII CCWGG 2 cut(s) 2371, 2661
EcoRV GATATC 5 cut(s) 65, 1093, 1471, 1726, 1791
EcoT14I CCWWGG 1 cut(s) 1685
EcoT22I ATGCAT 2 cut(s) 1010, 1251
EcoT38I GRGCYC 1 cut(s) 910
ErhI CCWWGG 1 cut(s) 1685
FalI AAGNNNNNCTT 2 cut(s) 799, 831
FaqI GGGAC 3 cut(s) 1853, 2039, 3173
FauI CCCGC 1 cut(s) 3342
FauNDI CATATG 3 cut(s) 1756, 2019, 2591
FblI GTMKAC 2 cut(s) 375, 877
Fnu4HI GCNGC 7 cut(s) 137, 306, 945, 1069, 1525, 2124, 3046
FokI GGATG 8 cut(s) 192, 233, 629, 945, 1729, 1940, 2348, 2529
FriOI GRGCYC 1 cut(s) 910
Fsp4HI GCNGC 7 cut(s) 137, 306, 945, 1069, 1525, 2124, 3046
FspBI CTAG 5 cut(s) 1206, 1224, 2573, 3075, 3117
GlaI GCGC 3 cut(s) 871, 3259, 3338
GluI GCNGC 7 cut(s) 137, 306, 945, 1069, 1525, 2124, 3046
GsaI CCCAGC 1 cut(s) 527
GsuI CTGGAG 1 cut(s) 1415
HaeIII GGCC 5 cut(s) 359, 908, 1163, 1193, 1339
HapII CCGG 8 cut(s) 597, 873, 1301, 2105, 2534, 3135, 3270, 3323
HgaI GACGC 1 cut(s) 1229
HhaI GCGC 3 cut(s) 872, 3260, 3339
Hin6I GCGC 3 cut(s) 870, 3258, 3337
HinP1I GCGC 3 cut(s) 870, 3258, 3337
HincII GTYRAC 1 cut(s) 2119
HindII GTYRAC 1 cut(s) 2119
HindIII AAGCTT 3 cut(s) 1578, 1640, 3236
HpaII CCGG 8 cut(s) 597, 873, 1301, 2105, 2534, 3135, 3270, 3323
HphI GGTGA 5 cut(s) 421, 1198, 1321, 2372, 2978
Hpy166II GTNNAC 6 cut(s) 376, 878, 1990, 2035, 2119, 2676
Hpy8I GTNNAC 6 cut(s) 376, 878, 1990, 2035, 2119, 2676
Hpy99I CGWCG 2 cut(s) 954, 3253
HpyCH4III ACNGT 9 cut(s) 721, 1818, 1966, 2030, 2198, 2349, 2722, 2870, 2984
HpyCH4IV ACGT 1 cut(s) 2577
HpyF10VI GCNNNNNNNGC 6 cut(s) 305, 893, 1358, 1367, 3092, 3345
HpySE526I ACGT 1 cut(s) 2577
HspAI GCGC 3 cut(s) 870, 3258, 3337
Kpn2I TCCGGA 2 cut(s) 2104, 3269
KspI CCGCGG 1 cut(s) 3049
LmnI GCTCC 3 cut(s) 422, 786, 2611
Lsp1109I GCAGC 5 cut(s) 148, 317, 931, 1055, 2110
LweI GCATC 8 cut(s) 76, 211, 671, 971, 1236, 1339, 1933, 1962
MaeI CTAG 5 cut(s) 1206, 1224, 2573, 3075, 3117
MaeII ACGT 1 cut(s) 2577
MaeIII GTNAC 3 cut(s) 2174, 2205, 2364
MfeI CAATTG 2 cut(s) 1098, 2705
MflI RGATCY 7 cut(s) 46, 560, 600, 1516, 2375, 2569, 2824
MhlI GDGCHC 3 cut(s) 427, 910, 3082
MlsI TGGCCA 1 cut(s) 1339
MluNI TGGCCA 1 cut(s) 1339
MlyI GAGTC 3 cut(s) 381, 1518, 3247
MmeI TCCRAC 5 cut(s) 373, 400, 1701, 1840, 3194
Mox20I TGGCCA 1 cut(s) 1339
Mph1103I ATGCAT 2 cut(s) 1010, 1251
MroI TCCGGA 2 cut(s) 2104, 3269
MroXI GAANNNNTTC 1 cut(s) 3205
MscI TGGCCA 1 cut(s) 1339
Msp20I TGGCCA 1 cut(s) 1339
MspA1I CMGCKG 1 cut(s) 3048
MspCI CTTAAG 1 cut(s) 2065
MspI CCGG 8 cut(s) 597, 873, 1301, 2105, 2534, 3135, 3270, 3323
MspR9I CCNGG 5 cut(s) 598, 2373, 2663, 3135, 3136
MunI CAATTG 2 cut(s) 1098, 2705
Mva1269I GAATGC 4 cut(s) 343, 872, 2165, 2735
MvaI CCWGG 2 cut(s) 2373, 2663
MvnI CGCG 3 cut(s) 3048, 3258, 3337
MwoI GCNNNNNNNGC 6 cut(s) 305, 893, 1358, 1367, 3092, 3345
NciI CCSGG 3 cut(s) 598, 3135, 3136
NdeI CATATG 3 cut(s) 1756, 2019, 2591
NlaIV GGNNCC 6 cut(s) 48, 595, 602, 908, 2098, 2954
NmeAIII GCCGAG 1 cut(s) 2189
NsiI ATGCAT 2 cut(s) 1010, 1251
NspI RCATGY 2 cut(s) 1825, 2280
NspV TTCGAA 2 cut(s) 2727, 3014
PagI TCATGA 1 cut(s) 333
PctI GAATGC 4 cut(s) 343, 872, 2165, 2735
PdmI GAANNNNTTC 1 cut(s) 3205
PflMI CCANNNNNTGG 1 cut(s) 1414
PkrI GCNGC 7 cut(s) 138, 307, 946, 1070, 1526, 2125, 3047
PleI GAGTC 3 cut(s) 380, 1517, 3247
PpsI GAGTC 3 cut(s) 380, 1517, 3247
Psp6I CCWGG 2 cut(s) 2371, 2661
PspFI CCCAGC 1 cut(s) 523
PspGI CCWGG 2 cut(s) 2371, 2661
PspN4I GGNNCC 6 cut(s) 48, 595, 602, 908, 2098, 2954
PspOMI GGGCCC 1 cut(s) 906
PspPI GGNCC 5 cut(s) 906, 907, 1162, 1192, 2600
PstI CTGCAG 1 cut(s) 2387
PsuI RGATCY 7 cut(s) 46, 560, 600, 1516, 2375, 2569, 2824
RsaI GTAC 3 cut(s) 60, 1820, 2840
RsaNI GTAC 3 cut(s) 59, 1819, 2839
SacII CCGCGG 1 cut(s) 3049
SatI GCNGC 7 cut(s) 137, 306, 945, 1069, 1525, 2124, 3046
Sau96I GGNCC 5 cut(s) 906, 907, 1162, 1192, 2600
SchI GAGTC 3 cut(s) 381, 1518, 3247
ScrFI CCNGG 5 cut(s) 598, 2373, 2663, 3135, 3136
SduI GDGCHC 3 cut(s) 427, 910, 3082
SfaNI GCATC 8 cut(s) 76, 211, 671, 971, 1236, 1339, 1933, 1962
SfcI CTRYAG 2 cut(s) 1157, 2383
Sfr303I CCGCGG 1 cut(s) 3049
SfuI TTCGAA 2 cut(s) 2727, 3014
SgrBI CCGCGG 1 cut(s) 3049
SinI GGWCC 1 cut(s) 2600
SmaI CCCGGG 1 cut(s) 3136
SmlI CTYRAG 3 cut(s) 266, 1643, 2065
SmoI CTYRAG 3 cut(s) 266, 1643, 2065
SsiI CCGC 6 cut(s) 1524, 1547, 2126, 3046, 3048, 3335
SspI AATATT 1 cut(s) 1882
SspMI CTAG 5 cut(s) 1206, 1224, 2573, 3075, 3117
StyD4I CCNGG 5 cut(s) 596, 2371, 2661, 3133, 3134
StyI CCWWGG 1 cut(s) 1685
TaaI ACNGT 9 cut(s) 721, 1818, 1966, 2030, 2198, 2349, 2722, 2870, 2984
TaiI ACGT 1 cut(s) 2580
TatI WGTACW 2 cut(s) 1818, 2838
TauI GCSGC 2 cut(s) 1527, 3048
TscAI CASTG 6 cut(s) 146, 411, 1534, 2035, 2086, 2699
TseI GCWGC 5 cut(s) 136, 305, 944, 1068, 2123
TspGWI ACGGA 5 cut(s) 393, 945, 2910, 3114, 3192
TspMI CCCGGG 1 cut(s) 3134
TspRI CASTG 6 cut(s) 146, 411, 1534, 2035, 2086, 2699
Van91I CCANNNNNTGG 1 cut(s) 1414
Vha464I CTTAAG 1 cut(s) 2065
VpaK11BI GGWCC 1 cut(s) 2600
XapI RAATTY 9 cut(s) 21, 169, 498, 654, 750, 1392, 2004, 3217, 3292
XbaI TCTAGA 1 cut(s) 2572
XceI RCATGY 2 cut(s) 1825, 2280
XcmI CCANNNNNNNNNTGG 1 cut(s) 1754
XmaI CCCGGG 1 cut(s) 3134
XmiI GTMKAC 2 cut(s) 375, 877
XmnI GAANNNNTTC 1 cut(s) 3205
XspI CTAG 5 cut(s) 1206, 1224, 2573, 3075, 3117
Zsp2I ATGCAT 2 cut(s) 1010, 1251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.