Rw5G039630

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000085
Physical Location & Seq
Forward (+)
69875461 .. 69883850
8390 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G039630.1

Sequence Viewer

Length: 2892 bp
ATGATCATTCAGTGCATGGAAGATCAGAGCACAATAGTCATCCCAATCTTTTATGATGTGGTTCCCTCTGATGTGCGACATCAGAAAGGGAGTTTTGCAAGAGCCTTCACTGAGCACCAAGAAAGGCTCGCGCAAGACGATGAGTACCCAAATAAGTTGAACCAGTGGAGAGTTGCTTTAACAAAAGTTGCAGGTCTCTCTGGCTTGGAATCAAACAAATATAGTTCTGACTTAGAACTTGTCAAAGGCATTGTGGAAACCGTGTGCGGTAAACTACGACCTATTGATGCTGAGTGGGCATTGTCGTCTACTGGCGATTTTGTAGCATTTGAAGCAACAAGACAAGCCATGGAGAAGGTAATGAGGGCACTTCAAGATGATGAGATCACTGCCATTGGGGTCTTTGGTATGGGAGGCGTTGGCAAGACAAGTATGGTGAAACATGTCGGTGCACAAGCCCGGAAAATTGGGCTCTTTAATTATGTGATTATGGCTGTCGTATCCCAAACACCTAGTTTGAGACATATTCAAGGCACACTGGCCGATCTGCTGGGACTAAAATTGTTGGAGGAGACAGAATTCGGAAGAGCGGTTAGATTGATGGAGAAAATAATGAAAGGAAATCGGATCCTTATAATCTTGGACGACATTTGGGAGAGAATAGATTTGTCTACTATTGGAATACCGAGCCATGATGAGCTTCAAAAGTGCAATTCCAAAATCTTATTCACTACAAGGAGATTGAATGTTTGCCATTCGATGGAAAGCCAAGCAAGCATTCCTCTTCCAGTCTTATCAGAAGATGATGCTTGGAACCTGTTTGTGAAAAAAGCAAGAAAGTTTTTTCATGAATCAACCAGCTTCTATGATGTAGCGAGGACTGTAGCTAAAGAATGTGCTGGTCTCCCAGTTGCATTGGTAGCGGTTGCAAGGGCACTTGGAGATAAAGACTTAGATGATTGGACGGAAGCAGCTCGACGACTAAAGGCTGCTCAACCTGTCAACTATGAAGATGAGAGATATGTGTTCAGATGTATAAAGTTGAGCTATGATTACTTGAGACCTGATGATAACAACATTGCCAAGTCATTCTTCTTGCTTTGTTGTCTATTCCCTGAAGATTTTGATATCGAGGTAGAAGACTTGTTGGTGTATGGGATTGGAAAAGGATTATTTCAAGACGCTGACACAATCAAAGAAGCCAGAGCCAAAGCCCATTTTGTGGTAAAGTACTTGAAAGCTTCTAGCTTGCTTTTGGACAGTGAATACAGCGGATTTGTGAGGATGCACGATGTCATTCGAGATGTGGCCATATCAATTTCATCATCGGATGATGGCCCACGGTTTTTGGTTAAAGCTGGTTGTAAGTTAAAGAATTGGCCAACAATTAATGTTGCACATGAAGACTACTCTGCAATATCGCTAATGAGGAATGAAATTGACGAGCTTCCTGAAAAGTTGTCTCATTGTTTTGAGATATATGGGTTTCCGAAAGGGCTGTTCTCAGTTTTCAATTTAGAAACCCTAATCTCCCACCTGATGGCCTCCGCCAACGAGCAGCGAGCCAGCGATCCCAGGCCGAGCTCCTCTTCAGTCTCTGCAAGCAGACCGATCCTGACTCGCTCTCACTCAAACTCGCTCACCTCCTCCAAATCTCGCCGGCGCAGGAGGCCCGGACCATGTCCGCCATTCTCCTCCGCAGGCAGCTCACCCGCGACGACTCCTATATCTGGCCCAGGCTCTCCCCGACGACGCAGTCCAGCCTCAATCGATTCTCCTGTCCTGGATAGGTTTCAGGACCTATTGCCGGCGATGATGAGGACACTCATGGAGTCTTTGAACAACGGGAACGAGGCCACGGCACAGGAGGCACTCGAGTTGTTCATCGAATTGGCTGGGACCGAGCCAAGGTTCCTCAGGAGGCAGATTGTGGAGGTGGTTGGCTCTATGCTGCGTGTTGCTGAAGCTGAGTCCTTGGAAGAAGGGACCAGACACTTGGCCATAGAGTTTGTAATCACTCTAGCCGAGGCCAGCGAGCGAGCCCCGGGGATGATGAGGAAGTTGCCGCAGTTTATTAGTAGGAGTGTTTGGATAGGCTTGCCATTTCCTTGGGTGGGAATACCATTGTTCCCGTCACGTCCGAGCAGTTGCTTGCCTATTTGGATTGCCCTTGCATGGATTGCTGAGGGTTGTTCTAAGGTAATGATAAAAAATTTGGAGCAAGTGGTGGCAATGGTCTTGAACTCGTTTCAAGATCCTCATCCTCGTGTAAGGTGGGCAGCTATTAATGCGATTGAACAGTTGTCTACTGATTTGGGCCCAGACCTGCACGTTCAATATCATCAACGGGTGCTTCCGGCATTAGCTGCTGCCATGGATGATTTTCAGAATCCTCGTGTGCAGATGGCTCCTTATGATCTTCTTTTTGTAGTTTTTGTTTTGCACAGAAGTTCTGGGAGATACACTTGCAACTCATTTAGACAAATGCCTCGTGTTTATGTTGGGTGCATGAAGTCAGGCGAAGTTTTCTCTGAGCCGAGTCAAAAGTGGTATGAACCAGAATGGTGGAAGGTTGGTGATAAAAAATCATATTTTCGCCATGCTTCAGCTCCTATCATCATTAGTTTCATCAAGGAACAAGCACTACAAAAAAGAATTGATGTAATTGCTCCTATCATCATTAGTTTCATCAAGGAACAAGCACTACAAAAAAGAATTGATGTAATTGGTCTGGCCAATTTTGTTCGAATAAATAGAGATATTCTCCGTACCTATGCCCATGATGATGTCAGTGTCGGATCTTGGTTAATTGGGCTTGATTTTAAACATCTTAATGACGCCAAGTTTTGCTGCTCCTCTTGGACAGCAGGAGCCATTTGTGCTGGGGTATGA

Protein Analysis

963

Amino Acids

107.99

Weight (kDa)

6.88

Isoelectric Point (pI)

47.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 2 - 96 6.7e-16 TIR domain
NB-ARC PF00931 115 - 278 1.3e-30 NB-ARC domain
TPR_IPO5 PF25780 592 - 647 4.8e-06 IPO5-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 635
AasI GACNNNNNNGTC 1 cut(s) 1755
Acc36I ACCTGC 2 cut(s) 182, 2334
AccB7I CCANNNNNTGG 3 cut(s) 760, 1222, 1540
AccBSI CCGCTC 1 cut(s) 590
AccI GTMKAC 3 cut(s) 308, 671, 2306
AccII CGCG 2 cut(s) 131, 1715
AciI CCGC 9 cut(s) 267, 590, 923, 1272, 1548, 1685, 1698, 1713, 2066
AclWI GGATC 6 cut(s) 622, 635, 1565, 1606, 2249, 2806
AcoI YGGCCR 5 cut(s) 540, 1308, 1379, 1998, 2733
AcsI RAATTY 2 cut(s) 578, 2212
AcuI CTGAAG 4 cut(s) 1137, 1575, 1983, 2589
AcyI GRCGYC 1 cut(s) 2838
AfaI GTAC 3 cut(s) 146, 1232, 2770
AfiI CCNNNNNNNGG 8 cut(s) 760, 1222, 1540, 1730, 1807, 1908, 2114, 2175
AflIII ACRYGT 1 cut(s) 442
AjiI CACGTC 1 cut(s) 2138
AjnI CCWGG 3 cut(s) 1574, 1735, 1782
AjuI GAANNNNNNNTTGG 6 cut(s) 710, 742, 762, 794, 1076, 1108
Alw21I GWGCWC 4 cut(s) 32, 117, 454, 1586
Alw26I GTCTC 7 cut(s) 200, 514, 566, 908, 1054, 1467, 1600
Alw44I GTGCAC 1 cut(s) 450
AlwI GGATC 6 cut(s) 622, 635, 1565, 1606, 2249, 2806
AlwNI CAGNNNCTG 1 cut(s) 1598
Ama87I CYCGRG 2 cut(s) 1874, 2044
ApaI GGGCCC 1 cut(s) 2321
ApaLI GTGCAC 1 cut(s) 450
ApeKI GCWGC 9 cut(s) 971, 989, 1558, 1704, 1951, 2279, 2366, 2369, 2850
ApoI RAATTY 2 cut(s) 578, 2212
AseI ATTAAT 2 cut(s) 1389, 2286
AspLEI GCGC 2 cut(s) 133, 1665
AspS9I GGNCC 9 cut(s) 1337, 1671, 1676, 1733, 1798, 1899, 1986, 2317, 2318
AsuC2I CCSGG 4 cut(s) 460, 1674, 2045, 2046
AsuHPI GGTGA 4 cut(s) 448, 1633, 1701, 2588
AsuII TTCGAA 1 cut(s) 2746
AvaI CYCGRG 2 cut(s) 1874, 2044
AvaII GGWCC 4 cut(s) 1676, 1798, 1899, 1986
AxyI CCTNAGG 1 cut(s) 1916
BaeGI GKGCMC 4 cut(s) 370, 454, 937, 2321
BalI TGGCCA 4 cut(s) 1310, 1381, 2000, 2735
BamHI GGATCC 1 cut(s) 627
BanII GRGCYC 4 cut(s) 474, 1586, 2044, 2321
BauI CACGAG 3 cut(s) 2265, 2394, 2490
BbsI GAAGAC 2 cut(s) 1146, 1410
Bbv12I GWGCWC 4 cut(s) 32, 117, 454, 1586
BbvCI CCTCAGC 1 cut(s) 2184
BbvI GCAGC 9 cut(s) 976, 983, 1570, 1716, 1938, 2291, 2353, 2356, 2837
BccI CCATC 5 cut(s) 595, 754, 1328, 1534, 2398
BceAI ACGGC 1 cut(s) 1875
BciT130I CCWGG 3 cut(s) 1576, 1737, 1784
BciVI GTATCC 1 cut(s) 511
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 4 cut(s) 460, 1674, 2045, 2046
BcoDI GTCTC 7 cut(s) 200, 514, 566, 908, 1054, 1467, 1600
BfaI CTAG 3 cut(s) 513, 1245, 2021
BfmI CTRYAG 1 cut(s) 882
BfuAI ACCTGC 2 cut(s) 182, 2334
BfuI GTATCC 1 cut(s) 511
BmcAI AGTACT 1 cut(s) 1232
Bme1390I CCNGG 7 cut(s) 460, 1576, 1674, 1737, 1784, 2045, 2046
Bme18I GGWCC 4 cut(s) 1676, 1798, 1899, 1986
BmeT110I CYCGRG 2 cut(s) 1874, 2044
BmgBI CACGTC 1 cut(s) 2138
BmgT120I GGNCC 9 cut(s) 1337, 1671, 1676, 1733, 1798, 1899, 1986, 2317, 2318
BmiI GGNNCC 9 cut(s) 63, 629, 815, 1900, 1913, 1987, 2319, 2409, 2872
BmrFI CCNGG 7 cut(s) 460, 1576, 1674, 1737, 1784, 2045, 2046
BmrI ACTGGG 1 cut(s) 902
BmsI GCATC 3 cut(s) 277, 796, 1275
BmuI ACTGGG 1 cut(s) 902
BpiI GAAGAC 2 cut(s) 1146, 1410
BplI GAGNNNNNCTC 2 cut(s) 2748, 2780
Bpu10I CCTNAGC 1 cut(s) 2184
Bpu14I TTCGAA 1 cut(s) 2746
BpuEI CTTGAG 1 cut(s) 1078
BpuMI CCSGG 4 cut(s) 460, 1674, 2045, 2046
Bsa29I ATCGAT 1 cut(s) 1770
BsaBI GATNNNNATC 1 cut(s) 2259
BsaHI GRCGYC 1 cut(s) 2838
BsaI GGTCTC 3 cut(s) 200, 908, 1054
Bsc4I CCNNNNNNNGG 8 cut(s) 760, 1222, 1540, 1730, 1807, 1908, 2114, 2175
Bse118I RCCGGY 2 cut(s) 1659, 1807
Bse1I ACTGG 5 cut(s) 163, 316, 543, 788, 908
Bse21I CCTNAGG 1 cut(s) 1916
Bse3DI GCAATG 2 cut(s) 1077, 2238
Bse8I GATNNNNATC 1 cut(s) 2259
BseBI CCWGG 3 cut(s) 1576, 1737, 1784
BseCI ATCGAT 1 cut(s) 1770
BseGI GGATG 6 cut(s) 39, 1290, 1336, 2055, 2260, 2383
BseJI GATNNNNATC 1 cut(s) 2259
BseLI CCNNNNNNNGG 8 cut(s) 760, 1222, 1540, 1730, 1807, 1908, 2114, 2175
BseMI GCAATG 2 cut(s) 1077, 2238
BseMII CTCAG 7 cut(s) 102, 282, 1518, 1930, 1959, 2175, 2523
BseNI ACTGG 5 cut(s) 163, 316, 543, 788, 908
BseRI GAGGAG 5 cut(s) 584, 1576, 1636, 1684, 2845
BseSI GKGCMC 4 cut(s) 370, 454, 937, 2321
BseXI GCAGC 9 cut(s) 976, 983, 1570, 1716, 1938, 2291, 2353, 2356, 2837
BseYI CCCAGC 3 cut(s) 550, 1895, 2882
BsgI GTGCAG 2 cut(s) 2312, 2420
Bsh1236I CGCG 2 cut(s) 131, 1715
BshVI ATCGAT 1 cut(s) 1770
BsiHKAI GWGCWC 4 cut(s) 32, 117, 454, 1586
BsiHKCI CYCGRG 2 cut(s) 1874, 2044
BsiSI CCGG 6 cut(s) 460, 1660, 1674, 1808, 2045, 2357
BslFI GGGAC 3 cut(s) 567, 1912, 1999
BslI CCNNNNNNNGG 8 cut(s) 760, 1222, 1540, 1730, 1807, 1908, 2114, 2175
BsmAI GTCTC 7 cut(s) 200, 514, 566, 908, 1054, 1467, 1600
BsmFI GGGAC 3 cut(s) 567, 1912, 1999
BsmI GAATGC 1 cut(s) 777
Bso31I GGTCTC 3 cut(s) 200, 908, 1054
BsoBI CYCGRG 2 cut(s) 1874, 2044
Bsp119I TTCGAA 1 cut(s) 2746
Bsp120I GGGCCC 1 cut(s) 2317
Bsp1286I GDGCHC 9 cut(s) 32, 117, 370, 454, 474, 937, 1586, 2044, 2321
Bsp19I CCATGG 2 cut(s) 348, 2373
BspACI CCGC 9 cut(s) 267, 590, 923, 1272, 1548, 1685, 1698, 1713, 2066
BspCNI CTCAG 7 cut(s) 103, 283, 1517, 1929, 1960, 2176, 2524
BspDI ATCGAT 1 cut(s) 1770
BspFNI CGCG 2 cut(s) 131, 1715
BspHI TCATGA 1 cut(s) 847
BspLI GGNNCC 9 cut(s) 63, 629, 815, 1900, 1913, 1987, 2319, 2409, 2872
BspMI ACCTGC 2 cut(s) 182, 2334
BspPI GGATC 6 cut(s) 622, 635, 1565, 1606, 2249, 2806
BspQI GCTCTTC 1 cut(s) 580
BspT104I TTCGAA 1 cut(s) 2746
BspTNI GGTCTC 3 cut(s) 200, 908, 1054
BsrBI CCGCTC 1 cut(s) 590
BsrDI GCAATG 2 cut(s) 1077, 2238
BsrFI RCCGGY 2 cut(s) 1659, 1807
BsrI ACTGG 5 cut(s) 163, 316, 543, 788, 908
BssAI RCCGGY 2 cut(s) 1659, 1807
BssNI GRCGYC 1 cut(s) 2838
BssSI CACGAG 3 cut(s) 2265, 2394, 2490
BssT1I CCWWGG 5 cut(s) 348, 1907, 1974, 2108, 2373
Bst2BI CACGAG 3 cut(s) 2265, 2394, 2490
Bst2UI CCWGG 3 cut(s) 1576, 1737, 1784
Bst4CI ACNGT 5 cut(s) 262, 883, 1262, 1344, 2301
Bst6I CTCTTC 3 cut(s) 580, 789, 1594
BstACI GRCGYC 1 cut(s) 2838
BstAPI GCANNNNNTGC 2 cut(s) 2180, 2366
BstBI TTCGAA 1 cut(s) 2746
BstDSI CCRYGG 4 cut(s) 348, 1340, 1857, 2373
BstF5I GGATG 6 cut(s) 39, 1290, 1336, 2055, 2260, 2383
BstFNI CGCG 2 cut(s) 131, 1715
BstHHI GCGC 2 cut(s) 133, 1665
BstMAI GTCTC 7 cut(s) 200, 514, 566, 908, 1054, 1467, 1600
BstNI CCWGG 3 cut(s) 1576, 1737, 1784
BstNSI RCATGY 1 cut(s) 446
BstSCI CCNGG 7 cut(s) 458, 1574, 1672, 1735, 1782, 2043, 2044
BstSFI CTRYAG 1 cut(s) 882
BstSLI GKGCMC 4 cut(s) 370, 454, 937, 2321
BstUI CGCG 2 cut(s) 131, 1715
BstV1I GCAGC 9 cut(s) 976, 983, 1570, 1716, 1938, 2291, 2353, 2356, 2837
BstV2I GAAGAC 2 cut(s) 1146, 1410
BstX2I RGATCY 3 cut(s) 627, 2254, 2798
BstXI CCANNNNNNTGG 3 cut(s) 1996, 2109, 2565
BstYI RGATCY 3 cut(s) 627, 2254, 2798
Bsu15I ATCGAT 1 cut(s) 1770
Bsu36I CCTNAGG 1 cut(s) 1916
BsuI GTATCC 1 cut(s) 511
BsuTUI ATCGAT 1 cut(s) 1770
BtgI CCRYGG 4 cut(s) 348, 1340, 1857, 2373
BtgZI GCGATG 1 cut(s) 1826
BtrI CACGTC 1 cut(s) 2138
BtsCI GGATG 6 cut(s) 39, 1290, 1336, 2055, 2260, 2383
BtsI GCAGTG 1 cut(s) 387
BtsIMutI CAGTG 7 cut(s) 17, 108, 170, 387, 536, 1267, 2797
BveI ACCTGC 2 cut(s) 182, 2334
CaiI CAGNNNCTG 1 cut(s) 1598
CciI TCATGA 1 cut(s) 847
CfoI GCGC 2 cut(s) 133, 1665
Cfr10I RCCGGY 2 cut(s) 1659, 1807
Cfr13I GGNCC 9 cut(s) 1337, 1671, 1676, 1733, 1798, 1899, 1986, 2317, 2318
Cfr9I CCCGGG 1 cut(s) 2044
ClaI ATCGAT 1 cut(s) 1770
CseI GACGC 3 cut(s) 1190, 1761, 2846
Csp6I GTAC 3 cut(s) 145, 1231, 2769
CspCI CAANNNNNGTGG 2 cut(s) 1329, 1364
CviQI GTAC 3 cut(s) 145, 1231, 2769
DraI TTTAAA 1 cut(s) 2824
DrdI GACNNNNNNGTC 1 cut(s) 1755
DseDI GACNNNNNNGTC 1 cut(s) 1755
EaeI YGGCCR 5 cut(s) 540, 1308, 1379, 1998, 2733
Eam1104I CTCTTC 3 cut(s) 580, 789, 1594
EarI CTCTTC 3 cut(s) 580, 789, 1594
EciI GGCGGA 2 cut(s) 1537, 1674
Ecl136II GAGCTC 1 cut(s) 1584
Eco130I CCWWGG 5 cut(s) 348, 1907, 1974, 2108, 2373
Eco24I GRGCYC 4 cut(s) 474, 1586, 2044, 2321
Eco31I GGTCTC 3 cut(s) 200, 908, 1054
Eco32I GATATC 1 cut(s) 1129
Eco47I GGWCC 4 cut(s) 1676, 1798, 1899, 1986
Eco53kI GAGCTC 1 cut(s) 1584
Eco57I CTGAAG 4 cut(s) 1137, 1575, 1983, 2589
Eco81I CCTNAGG 1 cut(s) 1916
Eco88I CYCGRG 2 cut(s) 1874, 2044
EcoICRI GAGCTC 1 cut(s) 1584
EcoO109I RGGNCCY 1 cut(s) 1798
EcoRI GAATTC 1 cut(s) 578
EcoRII CCWGG 3 cut(s) 1574, 1735, 1782
EcoRV GATATC 1 cut(s) 1129
EcoT14I CCWWGG 5 cut(s) 348, 1907, 1974, 2108, 2373
EcoT38I GRGCYC 4 cut(s) 474, 1586, 2044, 2321
ErhI CCWWGG 5 cut(s) 348, 1907, 1974, 2108, 2373
FalI AAGNNNNNCTT 2 cut(s) 1076, 1108
FaqI GGGAC 3 cut(s) 567, 1912, 1999
FauI CCCGC 1 cut(s) 1720
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 3 cut(s) 308, 671, 2306
FokI GGATG 6 cut(s) 26, 1297, 1343, 2062, 2247, 2390
FriOI GRGCYC 4 cut(s) 474, 1586, 2044, 2321
FspBI CTAG 3 cut(s) 513, 1245, 2021
GlaI GCGC 2 cut(s) 132, 1664
GsaI CCCAGC 3 cut(s) 554, 1899, 2886
HapII CCGG 6 cut(s) 460, 1660, 1674, 1808, 2045, 2357
HgaI GACGC 3 cut(s) 1190, 1761, 2846
HhaI GCGC 2 cut(s) 133, 1665
Hin1I GRCGYC 1 cut(s) 2838
Hin6I GCGC 2 cut(s) 131, 1663
HinP1I GCGC 2 cut(s) 131, 1663
HincII GTYRAC 1 cut(s) 1003
HindII GTYRAC 1 cut(s) 1003
HindIII AAGCTT 1 cut(s) 1239
HinfI GANTC 9 cut(s) 209, 851, 1618, 1720, 1772, 1832, 1970, 2389, 2539
HpaII CCGG 6 cut(s) 460, 1660, 1674, 1808, 2045, 2357
HphI GGTGA 4 cut(s) 448, 1633, 1701, 2588
Hpy166II GTNNAC 6 cut(s) 272, 309, 452, 672, 1003, 2307
Hpy8I GTNNAC 6 cut(s) 272, 309, 452, 672, 1003, 2307
Hpy99I CGWCG 4 cut(s) 981, 1720, 1752, 1755
HpyAV CCTTC 4 cut(s) 115, 349, 1976, 2563
HpyCH4III ACNGT 5 cut(s) 262, 883, 1262, 1344, 2301
HpyCH4IV ACGT 2 cut(s) 2137, 2331
HpySE526I ACGT 2 cut(s) 2137, 2331
Hsp92I GRCGYC 1 cut(s) 2838
HspAI GCGC 2 cut(s) 131, 1663
KroI GCCGGC 2 cut(s) 1659, 1807
KroNI GCCGGC 2 cut(s) 1661, 1809
Ksp22I TGATCA 1 cut(s) 3
LguI GCTCTTC 1 cut(s) 580
LmnI GCTCC 7 cut(s) 1589, 2218, 2413, 2614, 2674, 2858, 2870
Lsp1109I GCAGC 9 cut(s) 976, 983, 1570, 1716, 1938, 2291, 2353, 2356, 2837
LweI GCATC 3 cut(s) 277, 796, 1275
MaeI CTAG 3 cut(s) 513, 1245, 2021
MaeII ACGT 2 cut(s) 2137, 2331
MaeIII GTNAC 1 cut(s) 2133
MbiI CCGCTC 1 cut(s) 590
MflI RGATCY 3 cut(s) 627, 2254, 2798
MhlI GDGCHC 9 cut(s) 32, 117, 370, 454, 474, 937, 1586, 2044, 2321
MlsI TGGCCA 4 cut(s) 1310, 1381, 2000, 2735
MluNI TGGCCA 4 cut(s) 1310, 1381, 2000, 2735
MlyI GAGTC 5 cut(s) 1612, 1714, 1841, 1979, 2548
MmeI TCCRAC 2 cut(s) 546, 2776
Mox20I TGGCCA 4 cut(s) 1310, 1381, 2000, 2735
MreI CGCCGGCG 1 cut(s) 1659
MroNI GCCGGC 2 cut(s) 1659, 1807
MscI TGGCCA 4 cut(s) 1310, 1381, 2000, 2735
MseI TTAA 9 cut(s) 179, 477, 1353, 1370, 1389, 2286, 2807, 2823, 2832
MslI CAYNNNNRTG 4 cut(s) 447, 2265, 2784, 2832
Msp20I TGGCCA 4 cut(s) 1310, 1381, 2000, 2735
MspA1I CMGCKG 1 cut(s) 1272
MspI CCGG 6 cut(s) 460, 1660, 1674, 1808, 2045, 2357
MspR9I CCNGG 7 cut(s) 460, 1576, 1674, 1737, 1784, 2045, 2046
Mva1269I GAATGC 1 cut(s) 777
MvaI CCWGG 3 cut(s) 1576, 1737, 1784
MvnI CGCG 2 cut(s) 131, 1715
NaeI GCCGGC 2 cut(s) 1661, 1809
NciI CCSGG 4 cut(s) 460, 1674, 2045, 2046
NcoI CCATGG 2 cut(s) 348, 2373
NgoMIV GCCGGC 2 cut(s) 1659, 1807
NlaIV GGNNCC 9 cut(s) 63, 629, 815, 1900, 1913, 1987, 2319, 2409, 2872
NmeAIII GCCGAG 3 cut(s) 1605, 2050, 2562
NmuCI GTSAC 1 cut(s) 2133
NspI RCATGY 1 cut(s) 446
NspV TTCGAA 1 cut(s) 2746
PaeR7I CTCGAG 1 cut(s) 1874
PagI TCATGA 1 cut(s) 847
PciI ACATGT 1 cut(s) 442
PciSI GCTCTTC 1 cut(s) 580
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PctI GAATGC 1 cut(s) 777
PdiI GCCGGC 2 cut(s) 1661, 1809
PfeI GAWTC 4 cut(s) 209, 851, 1772, 2389
PflFI GACNNNGTC 2 cut(s) 1680, 1755
PflMI CCANNNNNTGG 3 cut(s) 760, 1222, 1540
PfoI TCCNGGA 1 cut(s) 1782
PleI GAGTC 5 cut(s) 1612, 1714, 1840, 1978, 2547
PpsI GAGTC 5 cut(s) 1612, 1714, 1840, 1978, 2547
PpuMI RGGWCCY 1 cut(s) 1798
PscI ACATGT 1 cut(s) 442
PshBI ATTAAT 2 cut(s) 1389, 2286
PsiI TTATAA 1 cut(s) 635
Psp124BI GAGCTC 1 cut(s) 1586
Psp5II RGGWCCY 1 cut(s) 1798
Psp6I CCWGG 3 cut(s) 1574, 1735, 1782
PspFI CCCAGC 3 cut(s) 550, 1895, 2882
PspGI CCWGG 3 cut(s) 1574, 1735, 1782
PspN4I GGNNCC 9 cut(s) 63, 629, 815, 1900, 1913, 1987, 2319, 2409, 2872
PspOMI GGGCCC 1 cut(s) 2317
PspPI GGNCC 9 cut(s) 1337, 1671, 1676, 1733, 1798, 1899, 1986, 2317, 2318
PspPPI RGGWCCY 1 cut(s) 1798
PspXI VCTCGAGB 1 cut(s) 1874
PsrI GAACNNNNNNTAC 4 cut(s) 2628, 2660, 2688, 2720
PstNI CAGNNNCTG 1 cut(s) 1598
PsuI RGATCY 3 cut(s) 627, 2254, 2798
PsyI GACNNNGTC 2 cut(s) 1680, 1755
RsaI GTAC 3 cut(s) 146, 1232, 2770
RsaNI GTAC 3 cut(s) 145, 1231, 2769
RseI CAYNNNNRTG 4 cut(s) 447, 2265, 2784, 2832
SacI GAGCTC 1 cut(s) 1586
SapI GCTCTTC 1 cut(s) 580
SaqAI TTAA 9 cut(s) 179, 477, 1353, 1370, 1389, 2286, 2807, 2823, 2832
Sau96I GGNCC 9 cut(s) 1337, 1671, 1676, 1733, 1798, 1899, 1986, 2317, 2318
ScaI AGTACT 1 cut(s) 1232
SchI GAGTC 5 cut(s) 1612, 1714, 1841, 1979, 2548
ScrFI CCNGG 7 cut(s) 460, 1576, 1674, 1737, 1784, 2045, 2046
SduI GDGCHC 9 cut(s) 32, 117, 370, 454, 474, 937, 1586, 2044, 2321
SfaNI GCATC 3 cut(s) 277, 796, 1275
SfcI CTRYAG 1 cut(s) 882
Sfr274I CTCGAG 1 cut(s) 1874
SfuI TTCGAA 1 cut(s) 2746
SgrAI CRCCGGYG 1 cut(s) 1659
SinI GGWCC 4 cut(s) 1676, 1798, 1899, 1986
SlaI CTCGAG 1 cut(s) 1874
SmaI CCCGGG 1 cut(s) 2046
SmiMI CAYNNNNRTG 4 cut(s) 447, 2265, 2784, 2832
SmlI CTYRAG 2 cut(s) 1057, 1874
SmoI CTYRAG 2 cut(s) 1057, 1874
SsiI CCGC 9 cut(s) 267, 590, 923, 1272, 1548, 1685, 1698, 1713, 2066
SspMI CTAG 3 cut(s) 513, 1245, 2021
SstI GAGCTC 1 cut(s) 1586
StyD4I CCNGG 7 cut(s) 458, 1574, 1672, 1735, 1782, 2043, 2044
StyI CCWWGG 5 cut(s) 348, 1907, 1974, 2108, 2373
TaaI ACNGT 5 cut(s) 262, 883, 1262, 1344, 2301
TaiI ACGT 2 cut(s) 2140, 2334
TaqI TCGA 8 cut(s) 758, 976, 1131, 1300, 1770, 1875, 1887, 2746
TaqII GACCGA 2 cut(s) 1624, 1916
TatI WGTACW 1 cut(s) 1230
TauI GCSGC 1 cut(s) 2068
TfiI GAWTC 4 cut(s) 209, 851, 1772, 2389
Tru1I TTAA 9 cut(s) 179, 477, 1353, 1370, 1389, 2286, 2807, 2823, 2832
Tru9I TTAA 9 cut(s) 179, 477, 1353, 1370, 1389, 2286, 2807, 2823, 2832
TscAI CASTG 7 cut(s) 17, 115, 170, 394, 543, 1267, 2797
TseFI GTSAC 1 cut(s) 2133
TseI GCWGC 9 cut(s) 971, 989, 1558, 1704, 1951, 2279, 2366, 2369, 2850
Tsp45I GTSAC 1 cut(s) 2133
TspGWI ACGGA 2 cut(s) 980, 2756
TspMI CCCGGG 1 cut(s) 2044
TspRI CASTG 7 cut(s) 17, 115, 170, 394, 543, 1267, 2797
Tth111I GACNNNGTC 2 cut(s) 1680, 1755
Van91I CCANNNNNTGG 3 cut(s) 760, 1222, 1540
VneI GTGCAC 1 cut(s) 450
VpaK11BI GGWCC 4 cut(s) 1676, 1798, 1899, 1986
VspI ATTAAT 2 cut(s) 1389, 2286
XapI RAATTY 2 cut(s) 578, 2212
XceI RCATGY 1 cut(s) 446
XhoI CTCGAG 1 cut(s) 1874
XmaI CCCGGG 1 cut(s) 2044
XmiI GTMKAC 3 cut(s) 308, 671, 2306
XspI CTAG 3 cut(s) 513, 1245, 2021
ZrmI AGTACT 1 cut(s) 1232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.