Rw5G037820

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000085
Physical Location & Seq
Reverse (-)
66648501 .. 66650296
1796 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G037820.1

Sequence Viewer

Length: 1665 bp
ATGAAAAACAAGCTTCATAAGCTTCCCGAATATTTGGTATGTTCAGAGCTCCAAATTTTATTGTTACAAAGTAATGCTGGTATACGTGACATCCCAGAAACCTTTCTTCAAAGTCCTAATGCGCTAAGGGTCTTTGATCTTAGCTTCACTAGCATTTCATCACTACCTTCATCATTCAGTTTCCTAACCAACCTTCAAGTTTTGAATTTGGATTTTTGCCATAATTTAAGTAACATATCCGTTCTAGGAAAACTTAAGAAACTTAAGATTCTTAGTATGAGAGAATTTCCTCTTAAGAAATTTCCAAAACAAATAGGAGATTTGACCAGTCTAAGGATGCTGGACGTCACTGGTAGAGATCTTGAAACAATCCCATCTAAAGTGATATCGAGGTTGTATAGATTGGAAGAACTGCACATGAAATTTCAATTTGGGAAGTGGGGAAGTAAAATTGAGGATGCAGTTGAAGAAACCAATGCTGACTTTGATGAGTTGACTGGCTTGTTGGATTTAAACATTTTGGAAGTTTACATATCTGATGCTGCATGCTTGCCTGAAAGTATTGAGTACTTCAAGCCGAATTGGGTAAAATTTGATATATGCATCAGCAGACACGTCCCTTACGTGTTGCTCCCTCAACATGATGGTCTTTCAAGAACCTTGACTCTTGACACAACAATCAGAAGCTTACCGTATTGGTTTATCAGCGCTGTGACAGAGAAGGTGGAGAAGCTAGAGTACACAGATTGCGAAGACTTGAATGACATCGTTAGGGAATATGACCATGGCAGATTACTTGCACTGAAATATCTCTCTGTGAACGGGAACCGTAAGAACTTGAAAGAGATAATCAACACAATGACACGGGTTTCAAATAAACCAGTTTTTGAGAACTTGGAAGAGTTGCATCTGCACCGCTTATATTGCCTCAAGGAGTTATGTGTTGGTGAGCTACCACCTGGGTCTCTACGCAAGCTCAAGTTCTTGAAAGTGCAAACTTGTCGTAGCTTGGTGAATGCACTTTTACCATCAACATTGTTGCAGAGACTACAAAATCTGGAAGAAATCATTTGTGTAAATCTGGAAGGAATGAAATATGTATTTGGATCAGATGAAGGGCTTGATCCAGAAAAGATTTTCTTGAGGAAATTGAGGGAGATGAGATTGTCTGCTCTATACGAACTAGTAAGCATATGTAATGGTCCTGCTCGGTGTGCAATCTTCCAGAATCTTAAAGTTTTGGCAATTTGCAAGTGCAAGGAATTGAAAAATCTCTTCACAATAGATGTAGCTGATTGTCTTTTTCAGTTGGAAGACCTTTCCATAGAGTGCTGTCCTAGCTTGGACAGAGTCATTGAAGCAAGCAATAGCTTGAACAGCAAGATTAAACTTCCGGCGTTGAAGAAGTTAGCTTTGATAGATCTTCCGGTGCTCACAAGCTTTTGCACTGAAAGTGGCAATATTGAAACTGAGTGCCCTTCATTGGAATACTTGTATGTGGAGAAGTGCCTCCAGTTTTCGTATTTTGCTTGCCACTTCGACAACAAGAACCAAGTCCACTTCAATGATAAACAACATTTGAGTTCTATAGTGAAAAGGCATGTCATAAATTCCAACTGTATTCTTTTCTATTCTTGGTGGGAGGAAGTGCATAATCGGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

554

Amino Acids

63.78

Weight (kDa)

6.39

Isoelectric Point (pI)

46.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 16 - 74 3.4e-08 Leucine rich repeat
LRR_14 PF23598 45 - 141 6.4e-13 Leucine-rich repeat region
LRR_RPS2 PF23247 299 - 445 1.6e-24 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 348
AccI GTMKAC 1 cut(s) 82
AciI CCGC 1 cut(s) 916
AclWI GGATC 2 cut(s) 1114, 1118
AcsI RAATTY 7 cut(s) 54, 205, 284, 299, 422, 590, 1609
AcyI GRCGYC 1 cut(s) 345
AfaI GTAC 2 cut(s) 569, 740
AfeI AGCGCT 1 cut(s) 709
AfiI CCNNNNNNNGG 2 cut(s) 333, 1483
AflII CTTAAG 3 cut(s) 254, 263, 293
AflIII ACRYGT 2 cut(s) 613, 624
AhlI ACTAGT 1 cut(s) 1183
AjiI CACGTC 1 cut(s) 616
AjnI CCWGG 1 cut(s) 958
Alw21I GWGCWC 2 cut(s) 51, 1434
Alw26I GTCTC 2 cut(s) 969, 1039
AlwI GGATC 2 cut(s) 1114, 1118
Aor51HI AGCGCT 1 cut(s) 709
ApeKI GCWGC 1 cut(s) 542
ApoI RAATTY 7 cut(s) 54, 205, 284, 299, 422, 590, 1609
Asp700I GAANNNNTTC 1 cut(s) 102
AspLEI GCGC 2 cut(s) 124, 710
AspS9I GGNCC 1 cut(s) 1202
AsuHPI GGTGA 2 cut(s) 959, 1024
AvaII GGWCC 1 cut(s) 1202
BaeGI GKGCMC 1 cut(s) 1478
BanII GRGCYC 1 cut(s) 51
BarI GAAGNNNNNNTAC 2 cut(s) 722, 754
BbsI GAAGAC 2 cut(s) 759, 1320
Bbv12I GWGCWC 2 cut(s) 51, 1434
BbvI GCAGC 1 cut(s) 529
BccI CCATC 3 cut(s) 382, 638, 1036
BcgI CGANNNNNNTGC 2 cut(s) 983, 1017
BciT130I CCWGG 1 cut(s) 960
BcoDI GTCTC 2 cut(s) 969, 1039
BcuI ACTAGT 1 cut(s) 1183
BfaI CTAG 5 cut(s) 150, 245, 734, 1184, 1338
BfmI CTRYAG 1 cut(s) 1587
BfoI RGCGCY 1 cut(s) 711
BfrI CTTAAG 3 cut(s) 254, 263, 293
BglII AGATCT 2 cut(s) 358, 1420
BisI GCNGC 1 cut(s) 543
BlsI GCNGC 1 cut(s) 544
BmcAI AGTACT 1 cut(s) 569
Bme1390I CCNGG 1 cut(s) 960
Bme18I GGWCC 1 cut(s) 1202
BmgBI CACGTC 1 cut(s) 616
BmgT120I GGNCC 1 cut(s) 1202
BmiI GGNNCC 1 cut(s) 827
BmrFI CCNGG 1 cut(s) 960
BmsI GCATC 5 cut(s) 327, 448, 529, 612, 916
BpiI GAAGAC 2 cut(s) 759, 1320
BpmI CTGGAG 1 cut(s) 1496
Bpu10I CCTNAGC 1 cut(s) 125
BpuEI CTTGAG 3 cut(s) 914, 962, 1162
BsaAI YACGTR 2 cut(s) 86, 625
BsaHI GRCGYC 1 cut(s) 345
BsaI GGTCTC 1 cut(s) 969
BsaJI CCNNGG 2 cut(s) 784, 959
BsaWI WCCGGW 1 cut(s) 1426
Bsc4I CCNNNNNNNGG 2 cut(s) 333, 1483
Bse1I ACTGG 5 cut(s) 327, 355, 502, 881, 1513
BseBI CCWGG 1 cut(s) 960
BseDI CCNNGG 2 cut(s) 784, 959
BseGI GGATG 3 cut(s) 90, 342, 463
BseLI CCNNNNNNNGG 2 cut(s) 333, 1483
BseMII CTCAG 1 cut(s) 1461
BseNI ACTGG 5 cut(s) 327, 355, 502, 881, 1513
BseSI GKGCMC 1 cut(s) 1478
BseXI GCAGC 1 cut(s) 529
BsgI GTGCAG 2 cut(s) 398, 896
BsiHKAI GWGCWC 2 cut(s) 51, 1434
BsiSI CCGG 2 cut(s) 1394, 1427
BslFI GGGAC 1 cut(s) 602
BslI CCNNNNNNNGG 2 cut(s) 333, 1483
BsmAI GTCTC 2 cut(s) 969, 1039
BsmFI GGGAC 1 cut(s) 602
BsmI GAATGC 1 cut(s) 1021
Bso31I GGTCTC 1 cut(s) 969
Bsp1286I GDGCHC 3 cut(s) 51, 1434, 1478
Bsp143I GATC 5 cut(s) 136, 358, 1106, 1123, 1420
Bsp19I CCATGG 1 cut(s) 784
BspACI CCGC 1 cut(s) 916
BspCNI CTCAG 1 cut(s) 1462
BspLI GGNNCC 1 cut(s) 827
BspPI GGATC 2 cut(s) 1114, 1118
BspTI CTTAAG 3 cut(s) 254, 263, 293
BspTNI GGTCTC 1 cut(s) 969
BsrI ACTGG 5 cut(s) 327, 355, 502, 881, 1513
BssECI CCNNGG 2 cut(s) 784, 959
BssMI GATC 5 cut(s) 136, 358, 1106, 1123, 1420
BssNAI GTATAC 1 cut(s) 83
BssNI GRCGYC 1 cut(s) 345
BssT1I CCWWGG 1 cut(s) 784
Bst1107I GTATAC 1 cut(s) 83
Bst2UI CCWGG 1 cut(s) 960
Bst4CI ACNGT 3 cut(s) 693, 830, 1619
Bst6I CTCTTC 2 cut(s) 894, 1280
BstACI GRCGYC 1 cut(s) 345
BstAFI CTTAAG 3 cut(s) 254, 263, 293
BstBAI YACGTR 2 cut(s) 86, 625
BstC8I GCNNGC 5 cut(s) 547, 551, 974, 1363, 1531
BstDEI CTNAG 5 cut(s) 125, 140, 272, 332, 1470
BstDSI CCRYGG 1 cut(s) 784
BstF5I GGATG 3 cut(s) 90, 342, 463
BstH2I RGCGCY 1 cut(s) 711
BstHHI GCGC 2 cut(s) 124, 710
BstKTI GATC 5 cut(s) 139, 361, 1109, 1126, 1423
BstMAI GTCTC 2 cut(s) 969, 1039
BstMBI GATC 5 cut(s) 136, 358, 1106, 1123, 1420
BstMWI GCNNNNNNNGC 7 cut(s) 19, 150, 924, 1214, 1338, 1377, 1657
BstNI CCWGG 1 cut(s) 960
BstNSI RCATGY 2 cut(s) 549, 1604
BstSCI CCNGG 1 cut(s) 958
BstSFI CTRYAG 1 cut(s) 1587
BstSLI GKGCMC 1 cut(s) 1478
BstV1I GCAGC 1 cut(s) 529
BstV2I GAAGAC 2 cut(s) 759, 1320
BstX2I RGATCY 2 cut(s) 358, 1420
BstYI RGATCY 2 cut(s) 358, 1420
BstZ17I GTATAC 1 cut(s) 83
BtgI CCRYGG 1 cut(s) 784
BtrI CACGTC 1 cut(s) 616
BtsCI GGATG 3 cut(s) 90, 342, 463
BtsIMutI CAGTG 3 cut(s) 348, 800, 1446
Cac8I GCNNGC 5 cut(s) 547, 551, 974, 1363, 1531
CfoI GCGC 2 cut(s) 124, 710
Cfr13I GGNCC 1 cut(s) 1202
Csp6I GTAC 2 cut(s) 568, 739
CviAII CATG 5 cut(s) 418, 546, 641, 785, 1601
CviQI GTAC 2 cut(s) 568, 739
DdeI CTNAG 5 cut(s) 125, 140, 272, 332, 1470
DpnI GATC 5 cut(s) 138, 360, 1108, 1125, 1422
DpnII GATC 5 cut(s) 136, 358, 1106, 1123, 1420
DraI TTTAAA 1 cut(s) 513
Eam1104I CTCTTC 2 cut(s) 894, 1280
EarI CTCTTC 2 cut(s) 894, 1280
Ecl136II GAGCTC 1 cut(s) 49
Eco130I CCWWGG 1 cut(s) 784
Eco24I GRGCYC 1 cut(s) 51
Eco31I GGTCTC 1 cut(s) 969
Eco32I GATATC 1 cut(s) 387
Eco47I GGWCC 1 cut(s) 1202
Eco47III AGCGCT 1 cut(s) 709
Eco53kI GAGCTC 1 cut(s) 49
EcoICRI GAGCTC 1 cut(s) 49
EcoRII CCWGG 1 cut(s) 958
EcoRV GATATC 1 cut(s) 387
EcoT14I CCWWGG 1 cut(s) 784
EcoT22I ATGCAT 1 cut(s) 605
EcoT38I GRGCYC 1 cut(s) 51
ErhI CCWWGG 1 cut(s) 784
FaeI CATG 5 cut(s) 421, 549, 644, 788, 1604
FalI AAGNNNNNCTT 2 cut(s) 1124, 1156
FaqI GGGAC 1 cut(s) 602
FatI CATG 5 cut(s) 417, 545, 640, 784, 1600
FauNDI CATATG 1 cut(s) 1193
FblI GTMKAC 1 cut(s) 82
Fnu4HI GCNGC 1 cut(s) 543
FokI GGATG 3 cut(s) 77, 349, 470
FriOI GRGCYC 1 cut(s) 51
Fsp4HI GCNGC 1 cut(s) 543
FspBI CTAG 5 cut(s) 150, 245, 734, 1184, 1338
GlaI GCGC 2 cut(s) 123, 709
GluI GCNGC 1 cut(s) 543
GsuI CTGGAG 1 cut(s) 1496
HaeII RGCGCY 1 cut(s) 711
HapII CCGG 2 cut(s) 1394, 1427
HhaI GCGC 2 cut(s) 124, 710
Hin1I GRCGYC 1 cut(s) 345
Hin1II CATG 5 cut(s) 421, 549, 644, 788, 1604
Hin6I GCGC 2 cut(s) 122, 708
HinP1I GCGC 2 cut(s) 122, 708
HincII GTYRAC 1 cut(s) 495
HindII GTYRAC 1 cut(s) 495
HindIII AAGCTT 4 cut(s) 11, 20, 685, 1438
HinfI GANTC 4 cut(s) 268, 664, 1228, 1350
HpaII CCGG 2 cut(s) 1394, 1427
HphI GGTGA 2 cut(s) 959, 1024
Hpy166II GTNNAC 6 cut(s) 83, 495, 529, 741, 820, 1558
Hpy188I TCNGA 4 cut(s) 46, 538, 683, 1111
Hpy8I GTNNAC 6 cut(s) 83, 495, 529, 741, 820, 1558
HpyAV CCTTC 6 cut(s) 177, 203, 715, 1079, 1109, 1488
HpyCH4III ACNGT 3 cut(s) 693, 830, 1619
HpyCH4IV ACGT 4 cut(s) 85, 345, 615, 624
HpyF10VI GCNNNNNNNGC 7 cut(s) 19, 150, 924, 1214, 1338, 1377, 1657
HpyF3I CTNAG 5 cut(s) 125, 140, 272, 332, 1470
HpySE526I ACGT 4 cut(s) 85, 345, 615, 624
Hsp92I GRCGYC 1 cut(s) 345
Hsp92II CATG 5 cut(s) 421, 549, 644, 788, 1604
HspAI GCGC 2 cut(s) 122, 708
Kzo9I GATC 5 cut(s) 136, 358, 1106, 1123, 1420
LmnI GCTCC 2 cut(s) 54, 636
Lsp1109I GCAGC 1 cut(s) 529
LweI GCATC 5 cut(s) 327, 448, 529, 612, 916
MaeI CTAG 5 cut(s) 150, 245, 734, 1184, 1338
MaeII ACGT 4 cut(s) 85, 345, 615, 624
MaeIII GTNAC 5 cut(s) 63, 86, 230, 346, 712
MalI GATC 5 cut(s) 138, 360, 1108, 1125, 1422
MboI GATC 5 cut(s) 136, 358, 1106, 1123, 1420
MflI RGATCY 2 cut(s) 358, 1420
MhlI GDGCHC 3 cut(s) 51, 1434, 1478
MlyI GAGTC 2 cut(s) 658, 1359
MmeI TCCRAC 3 cut(s) 486, 1290, 1638
MnlI CCTC 9 cut(s) 300, 384, 448, 645, 938, 1137, 1146, 1520, 1636
Mph1103I ATGCAT 1 cut(s) 605
MroXI GAANNNNTTC 1 cut(s) 102
MseI TTAA 7 cut(s) 227, 255, 264, 294, 512, 1233, 1386
MslI CAYNNNNRTG 1 cut(s) 1563
MspCI CTTAAG 3 cut(s) 254, 263, 293
MspI CCGG 2 cut(s) 1394, 1427
MspR9I CCNGG 1 cut(s) 960
Mva1269I GAATGC 1 cut(s) 1021
MvaI CCWGG 1 cut(s) 960
MwoI GCNNNNNNNGC 7 cut(s) 19, 150, 924, 1214, 1338, 1377, 1657
NcoI CCATGG 1 cut(s) 784
NdeI CATATG 1 cut(s) 1193
NdeII GATC 5 cut(s) 136, 358, 1106, 1123, 1420
NlaIII CATG 5 cut(s) 421, 549, 644, 788, 1604
NlaIV GGNNCC 1 cut(s) 827
NmuCI GTSAC 3 cut(s) 86, 346, 712
NsiI ATGCAT 1 cut(s) 605
NspI RCATGY 2 cut(s) 549, 1604
PaeI GCATGC 1 cut(s) 549
PcsI WCGNNNNNNNCGW 1 cut(s) 621
PctI GAATGC 1 cut(s) 1021
PdmI GAANNNNTTC 1 cut(s) 102
PfeI GAWTC 2 cut(s) 268, 1228
PflFI GACNNNGTC 1 cut(s) 1349
PkrI GCNGC 1 cut(s) 544
PleI GAGTC 2 cut(s) 658, 1358
PpsI GAGTC 2 cut(s) 658, 1358
Ppu21I YACGTR 2 cut(s) 86, 625
Psp124BI GAGCTC 1 cut(s) 51
Psp6I CCWGG 1 cut(s) 958
PspGI CCWGG 1 cut(s) 958
PspN4I GGNNCC 1 cut(s) 827
PspPI GGNCC 1 cut(s) 1202
PsuI RGATCY 2 cut(s) 358, 1420
PsyI GACNNNGTC 1 cut(s) 1349
RsaI GTAC 2 cut(s) 569, 740
RsaNI GTAC 2 cut(s) 568, 739
RseI CAYNNNNRTG 1 cut(s) 1563
SacI GAGCTC 1 cut(s) 51
SaqAI TTAA 7 cut(s) 227, 255, 264, 294, 512, 1233, 1386
SatI GCNGC 1 cut(s) 543
Sau3AI GATC 5 cut(s) 136, 358, 1106, 1123, 1420
Sau96I GGNCC 1 cut(s) 1202
ScaI AGTACT 1 cut(s) 569
SchI GAGTC 2 cut(s) 658, 1359
ScrFI CCNGG 1 cut(s) 960
SduI GDGCHC 3 cut(s) 51, 1434, 1478
SfaNI GCATC 5 cut(s) 327, 448, 529, 612, 916
SfcI CTRYAG 1 cut(s) 1587
SinI GGWCC 1 cut(s) 1202
SmiMI CAYNNNNRTG 1 cut(s) 1563
SmlI CTYRAG 6 cut(s) 254, 263, 293, 929, 977, 1141
SmoI CTYRAG 6 cut(s) 254, 263, 293, 929, 977, 1141
SpeI ACTAGT 1 cut(s) 1183
SphI GCATGC 1 cut(s) 549
SsiI CCGC 1 cut(s) 916
SspI AATATT 2 cut(s) 32, 1462
SspMI CTAG 5 cut(s) 150, 245, 734, 1184, 1338
SstI GAGCTC 1 cut(s) 51
StyD4I CCNGG 1 cut(s) 958
StyI CCWWGG 1 cut(s) 784
TaaI ACNGT 3 cut(s) 693, 830, 1619
TaiI ACGT 4 cut(s) 88, 348, 618, 627
TaqI TCGA 2 cut(s) 389, 1539
TatI WGTACW 2 cut(s) 567, 738
TfiI GAWTC 2 cut(s) 268, 1228
Tru1I TTAA 7 cut(s) 227, 255, 264, 294, 512, 1233, 1386
Tru9I TTAA 7 cut(s) 227, 255, 264, 294, 512, 1233, 1386
TscAI CASTG 3 cut(s) 355, 807, 1453
TseFI GTSAC 3 cut(s) 86, 346, 712
TseI GCWGC 1 cut(s) 542
Tsp45I GTSAC 3 cut(s) 86, 346, 712
TspDTI ATGAA 8 cut(s) 5, 17, 147, 159, 434, 1106, 1128, 1470
TspGWI ACGGA 1 cut(s) 229
TspRI CASTG 3 cut(s) 355, 807, 1453
Tth111I GACNNNGTC 1 cut(s) 1349
Vha464I CTTAAG 3 cut(s) 254, 263, 293
VpaK11BI GGWCC 1 cut(s) 1202
XapI RAATTY 7 cut(s) 54, 205, 284, 299, 422, 590, 1609
XceI RCATGY 2 cut(s) 549, 1604
XmiI GTMKAC 1 cut(s) 82
XmnI GAANNNNTTC 1 cut(s) 102
XspI CTAG 5 cut(s) 150, 245, 734, 1184, 1338
ZraI GACGTC 1 cut(s) 346
ZrmI AGTACT 1 cut(s) 569
Zsp2I ATGCAT 1 cut(s) 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.