Rmu_sc0003514.1_g000003

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003514.1
OG0000085
Physical Location & Seq
Forward (+)
19495 .. 26127
6633 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003514.1_g000003.1.cds

Sequence Viewer

Length: 4566 bp
atgaggtgccaagaaaatatcaccctcagcaccctatcagaagaagattcttggaccttatttgtgagaaatgcaggaaggttttctgaatctactaaattcgaggatgtagcgaggaaggtggctagagaatgtgctggtctcccaattgcggtgataaaagttgcaagggcactcggaaataaattcctggcggaatggaaagaagcagctcaacgactagagaagtcacaaactgccaaccctgatcatggagaagatgcattcagatgtataaagttgagctatgattacctgaaagaagaagattccaagtcatgcttcttgctttgttgcctatttccagaagactatgacatcccaatagaacacttgttcaagtatgcaatcgggaaaggattctttcgacatgccaaaacaattgaagaagccagaggaacagcagattcagtggtcaggtacctgaaagattctagcttgcttttggatagtgaaaaaaagggatgtgtaaggatgcatgatgtcatccgggatacggccctgaatattgcaaaatctgaagatgggcatgggtttttggtaaaagctggctgtggtttacaggattggccacgtcaattacatgaagggtctgagcagcaatcaagattgagagagatgacgttcttcaatttatctgccgtaagaagcatatgtaatggtcctgctcgacaagcaatgttccagaatcttaaaagtttgttcatctaccgttgcaactttctaggaggcctcttctcgtttgatgctgctcaatttctatttcaattggaagacctttttgtagacaattgtcctttactggaaagactaattgaagtaagcaaagaaacagtgagtgacaagaagactgttcttccaaaattgaagaacttaggtctggagaaacttcctaggttgtacggtggaagtggtactattgatatcaagtgtccttcattgaaacacttgagtgtgaaggactgcccccagttttcagcctttgcttccgacttccagagcaggaaccaagtgcaactcaatgataagttatattactttggtctaatacgcaaaagtactgaaagagaacttgttgaagccattgcggaatctgcgtactgtaaagtagaacctattgaaatcaatatgggagattttgaagcatttgaagcaacagagcaagccactatggaaattgagcctacaatgccaaagtcgagcaaagattttgaaacagttgaagcaacaacacaagccgccattggaaactctgaaccagctcagtggaagcatgatgtgtttttgagttttaggggtgaagacactcgcaagggttttctatcccatttataccatgaattgcaaaacacgaaagtaattagaacatttaaggatgatgaacaacttgagaaagggaaagatatttctcaaagtctactgacagcaattgaagaatcaaggtttgcgatcattgtcctgtcagaaaactatgcttcttctgcatggtgtttagatgaactcacgaagatttttcaatgcatggaaggcaaggatacaattctaccaattttttataatgtggatccttctgatatacgatatcagaagggtcaatttgaagtggcctttacaaagcatgaaagcaaggcaaggtatgacacagagaaggtgaagcagtggagagctgatttaacaaaagtgggaaatctctctggctggcattcaaagaaccataaatctgaaagggaactggttgaagacattgtgaaatttgtgctcaaaaaagtacctcaagcaccaaggggagattttgaagaatttgaatcaacaagagaagccatggatgaggttaagaagatgctgaaaggtgataagatcaccgtcattggggtctatggaagaggaggcgttggcaagacagccatggtgaaacatgtcagtgcacaagcccaaagaattgggctttttgatcatgttgttatggctgttatatcccgaaaccctgatttgcaaaaaattcaaggcgcattggtaaatcagctgggaatggaattggtggaggatgaagaaatagaaagagccagtagattgaaggagaggatcatgggaggaaaggggatccttattatcttggatgacatttgggaggtaataaatttgtcaagcattggaattcctagctatgacgagctccaaggatgcaattccaaggtcctattcaccacaaggatcaggaatctttgtcattctatggggtgccaagcacatattcctctcaatgtcctatcagaggaagattctttgaagttattcatgaagaagttgagaaagccttttcatgaatctaccatttcctatgatgaagagaggaacatagttagagaatgtgctggtctcccaaatgtactggaagaagctgcaagggcaattggacataaatacgtggaagaatggaatgaagcagtttcaaaggcatttggagataatgacaatgaattgcaaaaagcagctcaaaaactaaatgcatctcaacttgtccacactaaagatgagacagatacatttaaaagaatagagttaagctatcataccttgaggtttgatgatgccaagtcattgttcttgctttgctgcatgtttccagaaggttatgatatcccagttgaagaattgttgaagtatgggattggaaagggtttgcttcaatattctgacacattgcaagaagctagagacagagcaaattctatggttgcataccttaaagcttcggggttgcttttggatggcaagaagggatgcataaggatgcattgtggcatccgaaatgtggccatgtctattgctttgtctgaagaaggtcatgctttttttgtaaaagctggttgtgaattgaaggattggccaatggatgctcatgaaggctactcagcaatctcactaatgagaaacaaacttagcaaggttcccgaatctttggtatgttctgaactccaaattttactgctgcaaagtaatgctggtatatgtgacattccaaaaactttttttcaatgtccaaatgcactaagggtctttgatcttagctataccggtatttcagtactaccctcttcattcaatctcctcaccaacctccaagccttgtatttggattgttgcaataaaataaccgacatctccatacttgggaagctgaagaaagttgagattcttagcttgaaaaaatttcctcataaggagttgccgaaagagataggaaatttgaccagtctaaggatgctggacgtcactggtggttgtgttcacaaaattccatctaaagtgctatcgaggttgtatagactggaagaattgtacatgaaatgtgaatttggggactggggaagtaaagtcgaggaagcagtaggacaaacaaatgccgactttgatgagataattagcttgttccgtttaaacattttgaaggtttacatatctgatgcagcttgcttacccaaagaaattgggtactttgagccgacttgggtaaaatttgacatatgcatcagcagagatctctcttcggaaatgatgctcgctccggatgatcaaccttcaagaaccttgactcttgacagaacaatcaatggcttatcggattggtttatcaatgtggtgacacagaaagcagaaaaactagagtacagagagtgtaagggattaaacaacattcttaaggaatatgagcatgggagattacatggactcaagcatctctctgtaattgggtgccatgaaaacttggaagagttgataaatgcaacaacatgggatccaagtaaacctgtgttcgagagcttggaagagctgcatctccttcacttgcaacgcctcaaggagttatgtgttggcgaattacctctgggatctctgagcaatctcaagttattgaaagtgcaaacttgttgtaacttggtgaatgcacttttaccaccaaaattgttgcagagactaacaaatctagaaacagtattttgtgtaaatatggaacacatgaaatatttgtttggctctgaagggctggagcttggaacgactctcttgacaaaattgagcgaggtgagactctgcgctctcttcgaactaataagcatctggaatggtccggctccacatgcaatctttgggaatcttaaagttttggcagtttgcaagtgtacaaaactgaaaaatctattcccatccgatgtagctcgatctcttgtgcagttggaagacctctctgtagagcgctgccctaacttgggcatagttattgaagcatgcaccagcttgaaaaagatatatctgtttccagaattgaagaaattagctttgataaatcttccagagctcacatggttctgcaccgaaaatggtactgttcataccgagtgccctttattggaatacttgtatgtggatgagtgcccccagtttgtaaactctgcttctgcttctgttaaactcaacgatcaacaacatttgaattctctagcgaaaaggtatgtctctgtgttataa

Protein Analysis

1521

Amino Acids

172.08

Weight (kDa)

5.94

Isoelectric Point (pI)

39.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1596, 4564
AatII GACGTC 1 cut(s) 3312
Acc65I GGTACC 1 cut(s) 459
AccB1I GGYRCC 4 cut(s) 6, 459, 2277, 3792
AccI GTMKAC 2 cut(s) 825, 1456
AccIII TCCGGA 1 cut(s) 3604
AciI CCGC 4 cut(s) 152, 194, 1127, 1278
AclWI GGATC 9 cut(s) 1598, 1611, 2129, 2134, 2147, 2258, 3830, 3843, 3937
AcoI YGGCCR 3 cut(s) 608, 2853, 2924
AcuI CTGAAG 4 cut(s) 579, 2895, 3239, 4098
AcyI GRCGYC 1 cut(s) 3309
AfeI AGCGCT 1 cut(s) 4295
AflII CTTAAG 1 cut(s) 3737
AflIII ACRYGT 1 cut(s) 1954
AgeI ACCGGT 1 cut(s) 3113
AjiI CACGTC 1 cut(s) 614
AjnI CCWGG 1 cut(s) 189
AjuI GAANNNNNNNTTGG 8 cut(s) 305, 337, 2222, 2254, 2274, 2306, 2624, 2656
AleI CACNNNNGTG 1 cut(s) 990
Alw21I GWGCWC 4 cut(s) 1800, 1966, 2214, 4398
Alw26I GTCTC 7 cut(s) 146, 2420, 2567, 2748, 4006, 4120, 4558
Alw44I GTGCAC 1 cut(s) 1962
AlwI GGATC 9 cut(s) 1598, 1611, 2129, 2134, 2147, 2258, 3830, 3843, 3937
Aor13HI TCCGGA 1 cut(s) 3604
Aor51HI AGCGCT 1 cut(s) 4295
AoxI GGCC 6 cut(s) 537, 608, 769, 1644, 2853, 2924
ApaLI GTGCAC 1 cut(s) 1962
AsiGI ACCGGT 1 cut(s) 3113
Asp700I GAANNNNTTC 3 cut(s) 82, 398, 2330
Asp718I GGTACC 1 cut(s) 459
AspA2I CCTAGG 1 cut(s) 932
AspLEI GCGC 3 cut(s) 2048, 4136, 4296
AspS9I GGNCC 5 cut(s) 54, 538, 701, 2233, 4166
AsuC2I CCSGG 1 cut(s) 530
AsuII TTCGAA 1 cut(s) 4143
AvaII GGWCC 4 cut(s) 54, 701, 2233, 4166
AvrII CCTAGG 1 cut(s) 932
BaeGI GKGCMC 4 cut(s) 175, 1966, 4442, 4475
BalI TGGCCA 3 cut(s) 610, 2855, 2926
BamHI GGATCC 3 cut(s) 1603, 2139, 3835
BanI GGYRCC 4 cut(s) 6, 459, 2277, 3792
BanII GRGCYC 2 cut(s) 2214, 4398
BbsI GAAGAC 6 cut(s) 354, 819, 893, 1347, 1785, 4284
Bbv12I GWGCWC 4 cut(s) 1800, 1966, 2214, 4398
BbvCI CCTCAGC 1 cut(s) 26
BccI CCATC 4 cut(s) 557, 2801, 3346, 4252
BceAI ACGGC 2 cut(s) 552, 665
BciT130I CCWGG 1 cut(s) 191
BciVI GTATCC 2 cut(s) 526, 1567
BclI TGATCA 3 cut(s) 247, 1990, 3610
BcnI CCSGG 1 cut(s) 530
BcoDI GTCTC 7 cut(s) 146, 2420, 2567, 2748, 4006, 4120, 4558
BfmI CTRYAG 1 cut(s) 4287
BfoI RGCGCY 1 cut(s) 4297
BfrI CTTAAG 1 cut(s) 3737
BfuI GTATCC 2 cut(s) 526, 1567
BglII AGATCT 1 cut(s) 3577
BlnI CCTAGG 1 cut(s) 932
BmcAI AGTACT 2 cut(s) 1099, 3126
Bme1390I CCNGG 2 cut(s) 191, 530
Bme18I GGWCC 4 cut(s) 54, 701, 2233, 4166
BmgBI CACGTC 1 cut(s) 614
BmgT120I GGNCC 5 cut(s) 54, 538, 701, 2233, 4166
BmrFI CCNGG 2 cut(s) 191, 530
BmrI ACTGGG 4 cut(s) 1003, 2675, 3412, 4471
BmuI ACTGGG 4 cut(s) 1003, 2675, 3412, 4471
BoxI GACNNNNGTC 1 cut(s) 831
BpiI GAAGAC 6 cut(s) 354, 819, 893, 1347, 1785, 4284
BpmI CTGGAG 2 cut(s) 941, 4106
Bpu10I CCTNAGC 1 cut(s) 26
Bpu14I TTCGAA 1 cut(s) 4143
BpuEI CTTGAG 7 cut(s) 1009, 1448, 1797, 2635, 3755, 3881, 3929
BpuMI CCSGG 1 cut(s) 530
BsaAI YACGTR 1 cut(s) 2464
BsaHI GRCGYC 1 cut(s) 3309
BsaI GGTCTC 2 cut(s) 146, 2420
BsaJI CCNNGG 6 cut(s) 932, 1820, 1860, 1944, 2215, 2229
BsaWI WCCGGW 2 cut(s) 3113, 3604
Bse118I RCCGGY 1 cut(s) 3113
Bse3DI GCAATG 3 cut(s) 723, 1122, 2738
BseAI TCCGGA 1 cut(s) 3604
BseBI CCWGG 1 cut(s) 191
BseDI CCNNGG 6 cut(s) 932, 1820, 1860, 1944, 2215, 2229
BseMI GCAATG 3 cut(s) 723, 1122, 2738
BseMII CTCAG 5 cut(s) 40, 624, 1316, 2964, 3926
BseRI GAGGAG 2 cut(s) 1938, 3137
BseSI GKGCMC 4 cut(s) 175, 1966, 4442, 4475
BseYI CCCAGC 1 cut(s) 2062
BsgI GTGCAG 2 cut(s) 4289, 4393
BshFI GGCC 6 cut(s) 539, 610, 771, 1646, 2855, 2926
BshNI GGYRCC 4 cut(s) 6, 459, 2277, 3792
BshTI ACCGGT 1 cut(s) 3113
BsiHKAI GWGCWC 4 cut(s) 1800, 1966, 2214, 4398
BsiSI CCGG 4 cut(s) 529, 3114, 3605, 4169
BslFI GGGAC 1 cut(s) 3413
BsmAI GTCTC 7 cut(s) 146, 2420, 2567, 2748, 4006, 4120, 4558
BsmFI GGGAC 1 cut(s) 3413
BsmI GAATGC 3 cut(s) 263, 1741, 3988
BsnI GGCC 6 cut(s) 539, 610, 771, 1646, 2855, 2926
Bso31I GGTCTC 2 cut(s) 146, 2420
Bsp119I TTCGAA 1 cut(s) 4143
Bsp1286I GDGCHC 7 cut(s) 175, 1800, 1966, 2214, 4398, 4442, 4475
Bsp13I TCCGGA 1 cut(s) 3604
Bsp1407I TGTACA 2 cut(s) 3378, 4220
Bsp19I CCATGG 2 cut(s) 1860, 1944
BspACI CCGC 4 cut(s) 152, 194, 1127, 1278
BspANI GGCC 6 cut(s) 539, 610, 771, 1646, 2855, 2926
BspCNI CTCAG 5 cut(s) 39, 625, 1315, 2963, 3927
BspEI TCCGGA 1 cut(s) 3604
BspHI TCATGA 3 cut(s) 2334, 2359, 2938
BspPI GGATC 9 cut(s) 1598, 1611, 2129, 2134, 2147, 2258, 3830, 3843, 3937
BspQI GCTCTTC 1 cut(s) 3861
BspT104I TTCGAA 1 cut(s) 4143
BspT107I GGYRCC 4 cut(s) 6, 459, 2277, 3792
BspTI CTTAAG 1 cut(s) 3737
BspTNI GGTCTC 2 cut(s) 146, 2420
BsrDI GCAATG 3 cut(s) 723, 1122, 2738
BsrFI RCCGGY 1 cut(s) 3113
BsrGI TGTACA 2 cut(s) 3378, 4220
BssAI RCCGGY 1 cut(s) 3113
BssECI CCNNGG 6 cut(s) 932, 1820, 1860, 1944, 2215, 2229
BssNI GRCGYC 1 cut(s) 3309
BssT1I CCWWGG 6 cut(s) 932, 1820, 1860, 1944, 2215, 2229
Bst2UI CCWGG 1 cut(s) 191
Bst4CI ACNGT 9 cut(s) 752, 874, 892, 944, 1142, 1258, 1903, 4033, 4426
Bst6I CTCTTC 8 cut(s) 779, 1915, 2379, 3139, 3589, 3804, 3861, 4145
BstACI GRCGYC 1 cut(s) 3309
BstAFI CTTAAG 1 cut(s) 3737
BstAUI TGTACA 2 cut(s) 3378, 4220
BstBAI YACGTR 1 cut(s) 2464
BstBI TTCGAA 1 cut(s) 4143
BstC8I GCNNGC 7 cut(s) 479, 589, 1203, 1739, 3511, 3600, 4327
BstDSI CCRYGG 2 cut(s) 1860, 1944
BstENI CCTNNNNNAGG 1 cut(s) 2309
BstH2I RGCGCY 1 cut(s) 4297
BstHHI GCGC 3 cut(s) 2048, 4136, 4296
BstMAI GTCTC 7 cut(s) 146, 2420, 2567, 2748, 4006, 4120, 4558
BstMWI GCNNNNNNNGC 8 cut(s) 713, 1133, 1190, 1228, 1520, 1566, 2444, 4178
BstNI CCWGG 1 cut(s) 191
BstNSI RCATGY 5 cut(s) 413, 1958, 2659, 4181, 4329
BstPAI GACNNNNGTC 1 cut(s) 831
BstSCI CCNGG 2 cut(s) 189, 528
BstSFI CTRYAG 1 cut(s) 4287
BstSLI GKGCMC 4 cut(s) 175, 1966, 4442, 4475
BstV2I GAAGAC 6 cut(s) 354, 819, 893, 1347, 1785, 4284
BstX2I RGATCY 5 cut(s) 1603, 2139, 3577, 3835, 3929
BstXI CCANNNNNNTGG 2 cut(s) 1305, 1979
BstYI RGATCY 5 cut(s) 1603, 2139, 3577, 3835, 3929
BsuI GTATCC 2 cut(s) 526, 1567
BsuRI GGCC 6 cut(s) 539, 610, 771, 1646, 2855, 2926
BtgI CCRYGG 2 cut(s) 1860, 1944
BtrI CACGTC 1 cut(s) 614
BtsI GCAGTG 1 cut(s) 1703
BtsIMutI CAGTG 6 cut(s) 456, 879, 1310, 1703, 1966, 3312
Cac8I GCNNGC 7 cut(s) 479, 589, 1203, 1739, 3511, 3600, 4327
CciI TCATGA 3 cut(s) 2334, 2359, 2938
CfoI GCGC 3 cut(s) 2048, 4136, 4296
Cfr10I RCCGGY 1 cut(s) 3113
Cfr13I GGNCC 5 cut(s) 54, 538, 701, 2233, 4166
CspAI ACCGGT 1 cut(s) 3113
CspCI CAANNNNNGTGG 2 cut(s) 3984, 4019
DraI TTTAAA 2 cut(s) 2587, 3477
EaeI YGGCCR 3 cut(s) 608, 2853, 2924
Eam1104I CTCTTC 8 cut(s) 779, 1915, 2379, 3139, 3589, 3804, 3861, 4145
EarI CTCTTC 8 cut(s) 779, 1915, 2379, 3139, 3589, 3804, 3861, 4145
EciI GGCGGA 1 cut(s) 209
Ecl136II GAGCTC 2 cut(s) 2212, 4396
Eco130I CCWWGG 6 cut(s) 932, 1820, 1860, 1944, 2215, 2229
Eco147I AGGCCT 1 cut(s) 771
Eco24I GRGCYC 2 cut(s) 2214, 4398
Eco31I GGTCTC 2 cut(s) 146, 2420
Eco32I GATATC 3 cut(s) 964, 1622, 2677
Eco47I GGWCC 4 cut(s) 54, 701, 2233, 4166
Eco47III AGCGCT 1 cut(s) 4295
Eco53kI GAGCTC 2 cut(s) 2212, 4396
Eco57I CTGAAG 4 cut(s) 579, 2895, 3239, 4098
EcoICRI GAGCTC 2 cut(s) 2212, 4396
EcoNI CCTNNNNNAGG 1 cut(s) 2309
EcoO109I RGGNCCY 1 cut(s) 2233
EcoRI GAATTC 2 cut(s) 2193, 4531
EcoRII CCWGG 1 cut(s) 189
EcoRV GATATC 3 cut(s) 964, 1622, 2677
EcoT14I CCWWGG 6 cut(s) 932, 1820, 1860, 1944, 2215, 2229
EcoT22I ATGCAT 7 cut(s) 265, 519, 1562, 2548, 2825, 2835, 3569
EcoT38I GRGCYC 2 cut(s) 2214, 4398
ErhI CCWWGG 6 cut(s) 932, 1820, 1860, 1944, 2215, 2229
FalI AAGNNNNNCTT 2 cut(s) 305, 337
FaqI GGGAC 1 cut(s) 3413
FauNDI CATATG 2 cut(s) 692, 3563
FbaI TGATCA 3 cut(s) 247, 1990, 3610
FblI GTMKAC 2 cut(s) 825, 1456
FriOI GRGCYC 2 cut(s) 2214, 4398
GlaI GCGC 3 cut(s) 2047, 4135, 4295
GsaI CCCAGC 1 cut(s) 2066
GsuI CTGGAG 2 cut(s) 941, 4106
HaeII RGCGCY 1 cut(s) 4297
HaeIII GGCC 6 cut(s) 539, 610, 771, 1646, 2855, 2926
HapII CCGG 4 cut(s) 529, 3114, 3605, 4169
HhaI GCGC 3 cut(s) 2048, 4136, 4296
Hin1I GRCGYC 1 cut(s) 3309
Hin6I GCGC 3 cut(s) 2046, 4134, 4294
HinP1I GCGC 3 cut(s) 2046, 4134, 4294
HindIII AAGCTT 1 cut(s) 2787
HpaII CCGG 4 cut(s) 529, 3114, 3605, 4169
HpyCH4III ACNGT 9 cut(s) 752, 874, 892, 944, 1142, 1258, 1903, 4033, 4426
HpyCH4IV ACGT 4 cut(s) 613, 662, 2463, 3309
HpyF10VI GCNNNNNNNGC 8 cut(s) 713, 1133, 1190, 1228, 1520, 1566, 2444, 4178
HpySE526I ACGT 4 cut(s) 613, 662, 2463, 3309
Hsp92I GRCGYC 1 cut(s) 3309
HspAI GCGC 3 cut(s) 2046, 4134, 4294
Kpn2I TCCGGA 1 cut(s) 3604
KpnI GGTACC 1 cut(s) 463
Ksp22I TGATCA 3 cut(s) 247, 1990, 3610
LguI GCTCTTC 1 cut(s) 3861
LmnI GCTCC 4 cut(s) 2217, 3607, 4087, 4177
MaeII ACGT 4 cut(s) 613, 662, 2463, 3309
MaeIII GTNAC 6 cut(s) 228, 878, 3050, 3310, 3679, 3971
MfeI CAATTG 6 cut(s) 147, 420, 806, 829, 1467, 2448
MflI RGATCY 5 cut(s) 1603, 2139, 3577, 3835, 3929
MhlI GDGCHC 7 cut(s) 175, 1800, 1966, 2214, 4398, 4442, 4475
MlsI TGGCCA 3 cut(s) 610, 2855, 2926
MluNI TGGCCA 3 cut(s) 610, 2855, 2926
MlyI GAGTC 4 cut(s) 3625, 3762, 4093, 4122
MmeI TCCRAC 2 cut(s) 1053, 4254
Mox20I TGGCCA 3 cut(s) 610, 2855, 2926
Mph1103I ATGCAT 7 cut(s) 265, 519, 1562, 2548, 2825, 2835, 3569
MroI TCCGGA 1 cut(s) 3604
MroXI GAANNNNTTC 3 cut(s) 82, 398, 2330
MscI TGGCCA 3 cut(s) 610, 2855, 2926
MslI CAYNNNNRTG 4 cut(s) 268, 990, 1959, 2828
Msp20I TGGCCA 3 cut(s) 610, 2855, 2926
MspA1I CMGCKG 1 cut(s) 2062
MspCI CTTAAG 1 cut(s) 3737
MspI CCGG 4 cut(s) 529, 3114, 3605, 4169
MspR9I CCNGG 2 cut(s) 191, 530
MssI GTTTAAAC 1 cut(s) 3477
MunI CAATTG 6 cut(s) 147, 420, 806, 829, 1467, 2448
Mva1269I GAATGC 3 cut(s) 263, 1741, 3988
MvaI CCWGG 1 cut(s) 191
MwoI GCNNNNNNNGC 8 cut(s) 713, 1133, 1190, 1228, 1520, 1566, 2444, 4178
NciI CCSGG 1 cut(s) 530
NcoI CCATGG 2 cut(s) 1860, 1944
NdeI CATATG 2 cut(s) 692, 3563
NmuCI GTSAC 5 cut(s) 228, 878, 3050, 3310, 3679
NsiI ATGCAT 7 cut(s) 265, 519, 1562, 2548, 2825, 2835, 3569
NspI RCATGY 5 cut(s) 413, 1958, 2659, 4181, 4329
NspV TTCGAA 1 cut(s) 4143
OliI CACNNNNGTG 1 cut(s) 990
PaeI GCATGC 1 cut(s) 4329
PagI TCATGA 3 cut(s) 2334, 2359, 2938
PceI AGGCCT 1 cut(s) 771
PciI ACATGT 1 cut(s) 1954
PciSI GCTCTTC 1 cut(s) 3861
PctI GAATGC 3 cut(s) 263, 1741, 3988
PdmI GAANNNNTTC 3 cut(s) 82, 398, 2330
PfoI TCCNGGA 1 cut(s) 528
PinAI ACCGGT 1 cut(s) 3113
PleI GAGTC 4 cut(s) 3625, 3762, 4093, 4122
PmeI GTTTAAAC 1 cut(s) 3477
PpsI GAGTC 4 cut(s) 3625, 3762, 4093, 4122
Ppu21I YACGTR 1 cut(s) 2464
PpuMI RGGWCCY 1 cut(s) 2233
PscI ACATGT 1 cut(s) 1954
PshAI GACNNNNGTC 1 cut(s) 831
PsiI TTATAA 2 cut(s) 1596, 4564
Psp124BI GAGCTC 2 cut(s) 2214, 4398
Psp5II RGGWCCY 1 cut(s) 2233
Psp6I CCWGG 1 cut(s) 189
PspFI CCCAGC 1 cut(s) 2062
PspGI CCWGG 1 cut(s) 189
PspPI GGNCC 5 cut(s) 54, 538, 701, 2233, 4166
PspPPI RGGWCCY 1 cut(s) 2233
PsuI RGATCY 5 cut(s) 1603, 2139, 3577, 3835, 3929
PvuII CAGCTG 1 cut(s) 2062
RseI CAYNNNNRTG 4 cut(s) 268, 990, 1959, 2828
SacI GAGCTC 2 cut(s) 2214, 4398
SapI GCTCTTC 1 cut(s) 3861
Sau96I GGNCC 5 cut(s) 54, 538, 701, 2233, 4166
ScaI AGTACT 2 cut(s) 1099, 3126
SchI GAGTC 4 cut(s) 3625, 3762, 4093, 4122
ScrFI CCNGG 2 cut(s) 191, 530
SduI GDGCHC 7 cut(s) 175, 1800, 1966, 2214, 4398, 4442, 4475
SfcI CTRYAG 1 cut(s) 4287
SfuI TTCGAA 1 cut(s) 4143
SinI GGWCC 4 cut(s) 54, 701, 2233, 4166
SmiMI CAYNNNNRTG 4 cut(s) 268, 990, 1959, 2828
SmlI CTYRAG 8 cut(s) 988, 1427, 1812, 2614, 3737, 3770, 3896, 3944
SmoI CTYRAG 8 cut(s) 988, 1427, 1812, 2614, 3737, 3770, 3896, 3944
SphI GCATGC 1 cut(s) 4329
SseBI AGGCCT 1 cut(s) 771
SsiI CCGC 4 cut(s) 152, 194, 1127, 1278
SspI AATATT 3 cut(s) 547, 2729, 4064
SstI GAGCTC 2 cut(s) 2214, 4398
StuI AGGCCT 1 cut(s) 771
StyD4I CCNGG 2 cut(s) 189, 528
StyI CCWWGG 6 cut(s) 932, 1820, 1860, 1944, 2215, 2229
TaaI ACNGT 9 cut(s) 752, 874, 892, 944, 1142, 1258, 1903, 4033, 4426
TaiI ACGT 4 cut(s) 616, 665, 2466, 3312
TaqI TCGA 9 cut(s) 102, 406, 709, 1238, 3353, 3417, 3855, 4143, 4258
TatI WGTACW 6 cut(s) 1097, 2425, 3124, 3378, 3705, 4220
TauI GCSGC 1 cut(s) 1280
TscAI CASTG 6 cut(s) 456, 879, 1310, 1703, 1966, 3319
TseFI GTSAC 5 cut(s) 228, 878, 3050, 3310, 3679
Tsp45I GTSAC 5 cut(s) 228, 878, 3050, 3310, 3679
TspGWI ACGGA 1 cut(s) 3461
TspRI CASTG 6 cut(s) 456, 879, 1310, 1703, 1966, 3319
Vha464I CTTAAG 1 cut(s) 3737
VneI GTGCAC 1 cut(s) 1962
VpaK11BI GGWCC 4 cut(s) 54, 701, 2233, 4166
XagI CCTNNNNNAGG 1 cut(s) 2309
XbaI TCTAGA 1 cut(s) 4024
XceI RCATGY 5 cut(s) 413, 1958, 2659, 4181, 4329
XcmI CCANNNNNNNNNTGG 1 cut(s) 4398
XmaJI CCTAGG 1 cut(s) 932
XmiI GTMKAC 2 cut(s) 825, 1456
XmnI GAANNNNTTC 3 cut(s) 82, 398, 2330
ZraI GACGTC 1 cut(s) 3310
ZrmI AGTACT 2 cut(s) 1099, 3126
Zsp2I ATGCAT 7 cut(s) 265, 519, 1562, 2548, 2825, 2835, 3569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.