Rmu_sc0000826.1_g000016

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000826.1
OG0000085
Physical Location & Seq
Forward (+)
49186 .. 56053
6868 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000826.1_g000016.1.cds

Sequence Viewer

Length: 4647 bp
atggcttctccttcgtctttgtcatcatccggaggcagtttcaaatacgacgttttcatcaatttcagaggcgaagacacacgtaggtgtttcgtctgccatctttaccaagctctgaaacagaaagcactcgagcccttcatggattccgaagagcttagaacaggcgacgacctttcggggcgattcaaagccattcaaggctcggcgctttcgattgtagttttctctaaaaactacgcatcttctaaatggtgcttaaaagaactggtccagatcttggagtgtatggataggcggagccagattgtggtgcccattttctatgaagtagatccctcccgtgttcgcaagcctatggcaagttttgcacaagcttttgcaaaacatgaaagcgattccaatgtcgacaaagaagagttgcagagctggaagtccagtttaaggagagccagcaatttatctggctgggattccagaaattatcgggatgatacccagcttatcagcagcattgtagaagacattttactgaaattgatccgcatctcatcaactaaaaccgattggaatgcatatcgttatgcctcgacccctcgaaaatttgctgcatatcttccttcatcagttgaatcatcattattttcagctcgtccgtggatatatgatgtgtttttcagtgcagatactcgatgggatgtaaaagattttacatcctatttatacagtcggctgctcgagaggggaatcacaacgttcaggaatgattcagggcttgggcctgaagtacgggcaattgaaggatcaaggttggcaattgttgttctgtcccaaaaatatgctttttctactttgcgtttggaggaacttacaaagatttgtcagttcatggaggatggccacagaattcttccagttttccatgaggtcaaccctagtgacgtaagaaatcaaaaggggcgttttcaacatgctttcaccaagcatgaaaaatctgggcaatacgatccgcagaaggtgcagcagtggagagatgctgtaagaaaagtggccagtttgtctgggtggtttgtacaggatttcaagattgacaaagatcttgttgaagccattgtggcctctgtgtgcagaaaactacatccttctattgaaacttctatagaaattgagcctaaaatctcccaagaaattgctgtatctcttccttcatcagctgaaccatcatcaacagctcaacggtggaagtatgatgtgtttttgagttacaggggtggtgacactccaatgaattttataccggatttctacgatgggctgcaaaagagaggaattaaaacattcacgtatgatcgagatacaagagagtatgctcattatcccattttcagagcagcaattgaagagtcaaggcatgcagtcgtggttctcacggaaaagtatgcttgttctactcggtctttggaggaacttacaatgatatgtgagtgcatgaaagaccgtaacagaattcttccagttttttataatgtgaaaccctctgatatacagtaccggaagaggagtttcggagaggctttcgataagcatgaaagctctgggcgacacacatcagacaaggtgaagcgctggaaagatgttttaaaccatgtagccagtctctctgggtggcatacaatactttacaagaccgatagagaacttgtggacaatattctggattttgtgtacagtgaagtacaccgtattgaaattgagtttacaatgtccactggagattttgaagcatttgaatcaacaagaaaagccatgaaggacgttatgaaggcgcttacagatgatgaggtcactatcgttggggtctacggaaagggaggtgttggaaaaacaaccatggttgaacatgtcagtgaagcagttaagaataatgggatgttccatcatgtgatcacggttgttgtatccagaagccctgacttgagaaaaattcaaggcacattggcagacctattgggtgtcatattggaggacgagacagaaattggaagatcccgtagattgatgcagaagataaggagaggaaataggatccttataatcttggatgacatttgggagagaatagatttgaccgacataggaattcccagctgccacgagcttcaaagatgcaattccaaagtcctactcaccacaaggatacagaatgtttgtcatgtcatggagtgtcaatcaaacatttctcttgatgttttatcagaagcagattcttggaccttgtttgtgaacaaaacaaaacagtcttttgaacccaccatttttaatttttatgatgttgcaagggaggtagctagagattgtgctggtcttccagttgcactgatagcagttgcaaatgcacttgttggtaagtgtttggatgactggaaggaagcagctcgacgactaagggcttctcaaactgccaaccctgaagatgaggaatttgtgttcagttgtctaaaattaagctatgattacttaaaatctgatgatgccaagtcatgcttcttgctttgttgcctattcccagaagactatgatatccaaattgaagacttgctaaaatatgggatagggaaaggattgtttcaagatgtcaccactatgcatgatgccagagctagtgcatattcggtggtcaagtaccttaaagattgtggcttgcttttgaaatctgaacaggacggatgtgtaaagatgcatgatgtcattcgggatgtggccataaggatttcgtcatctgaagatggcccactgttttttgtacaagctggttgtcaattaaaggaatggccaagtattagtgcacatgaaggctactctgctatctcgctaattaggaataaaatttgcaagcttcctgaagagttggtatgtccgaaactccttattttgttcctgcaaagtaatgtttgcttgaatgagattccagaacatttctttcgaagtttgaatgtgttgagggtattagatctaagccgaaccagaatttcgttactacccacatcattcaatctcttgatgaacctccacaccttgtatctagatggttgtttatcaatcataaataatatatctgcactcggaagtttagaaaaacttgagattcttagtatgagagaccttcctcttgtggaattgccgaaagaattaggacatttgagtaatctaaggatgttggacatcactggtgaatatgttgatatagttccctctgaagtgatttgcaaccttcatagtttagaagaactatacatgcagtgtacttttgcagactggaggagtaaagttgggaggaatgaggtgaacgagagcaaagttgcggaagcaggggaagaaaatattgctgcctttgatgagttaactagtttgtcaaatttaaacattttgaaggttttcatatctgatgcaaaatgtttacctaaacatgttgagtttcagctaaattggataaactttgatatatgtatcagcagaaacccgtcagttagaaaagtgtccttggtttcaagttcttcacctgcttatacaagggttttgactcttggtacaacgatcaatgccttaccgtattggtttatcgatgtggtgacaaggaaagcagagaaactacagtacataatgtgttggggattagataacattattgtggaatatcaccttgggagtttagatgcactgaaatatttatctgttattgggcccaatgagaacttgaaagtcttgatgaaatgctttcgaaatgaacctgtgtttcagaacttagaagaactgcatctcattcaagtggatagcctgatggagttatgtgttggtgaattaccacctgggtctctatgcaatcttaggttattgaaggtgaaacattgttatgaattggtggatgcacttctatcgtcacaattgctgcagagattacaaaatctggaaaaactgatttgtgaagagatggatgaattggaatatgtttttggatgtgaagggcttaagccagaacatattattttgccagaattgaaagagatgagattggaaaatctagggaaactaataaagatatggaatggtcctgctccttgtgccattttccaaaaccttcagagtttggtagtttcaggatgctttgaattggaaaatctcttcacagctgatgtagctcagtgtcttaggcaattggaagatctttcagtagagctttgcccattattggacagagttattgaggcaagcaaggaaacaatatacagcaagattgtttttcccgaactgaagaacttagctttgataaagctaccacagctcacaaggttctatggtaataccggaagtgctattgagtgccctttattagaatacttatatgtggagaggtgcccccagttctcaacacctgacttccacagcaggaacaaattgcctccaactgatattggaaacactttgtctgagccaaggttcactgttggcatggtagtgattaacaacatcggcgtcatggttgctaactccaaatctgcttgggtgggttatgataaattgacaatatccagcttaatgggaagtgatcatgaagcacagaagaaatgctccaatttggctagtaagtga

Protein Analysis

1548

Amino Acids

176.08

Weight (kDa)

5.9

Isoelectric Point (pI)

48.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1524, 2093
AarI CACCTGC 1 cut(s) 3568
Acc36I ACCTGC 1 cut(s) 3568
AccB1I GGYRCC 2 cut(s) 313, 4410
AccB7I CCANNNNNTGG 2 cut(s) 280, 310
AccI GTMKAC 2 cut(s) 408, 1860
AccIII TCCGGA 1 cut(s) 29
AciI CCGC 4 cut(s) 298, 544, 1010, 3362
AclI AACGTT 1 cut(s) 755
AclWI GGATC 7 cut(s) 329, 535, 811, 1001, 2040, 2080, 2093
AcoI YGGCCR 4 cut(s) 898, 1050, 2760, 2831
AcuI CTGAAG 7 cut(s) 804, 2490, 2802, 2922, 3276, 4121, 4328
AcyI GRCGYC 1 cut(s) 4530
AfeI AGCGCT 1 cut(s) 1625
AfiI CCNNNNNNNGG 4 cut(s) 280, 310, 444, 4246
AflII CTTAAG 1 cut(s) 4025
AflIII ACRYGT 3 cut(s) 80, 1900, 3466
AhlI ACTAGT 1 cut(s) 3404
AjnI CCWGG 1 cut(s) 3865
AjuI GAANNNNNNNTTGG 6 cut(s) 2528, 2560, 3684, 3716, 3993, 4025
AleI CACNNNNGTG 1 cut(s) 85
AloI GAACNNNNNNTCC 2 cut(s) 2471, 2503
Alw21I GWGCWC 1 cut(s) 2848
Alw26I GTCTC 4 cut(s) 1661, 2024, 3153, 3876
Alw44I GTGCAC 1 cut(s) 2844
AlwI GGATC 7 cut(s) 329, 535, 811, 1001, 2040, 2080, 2093
Ama87I CYCGRG 2 cut(s) 131, 737
Aor13HI TCCGGA 1 cut(s) 29
Aor51HI AGCGCT 1 cut(s) 1625
AoxI GGCC 8 cut(s) 779, 898, 1050, 1116, 2760, 2788, 2831, 3740
ApaI GGGCCC 1 cut(s) 3744
ApaLI GTGCAC 1 cut(s) 2844
Asp700I GAANNNNTTC 1 cut(s) 3434
AspLEI GCGC 3 cut(s) 211, 1626, 1828
AspS9I GGNCC 7 cut(s) 271, 779, 2271, 2789, 3740, 3741, 4106
AsuII TTCGAA 2 cut(s) 2983, 3778
AvaI CYCGRG 2 cut(s) 131, 737
AvaII GGWCC 3 cut(s) 271, 2271, 4106
BaeGI GKGCMC 5 cut(s) 318, 2848, 3744, 4382, 4415
BalI TGGCCA 4 cut(s) 900, 1052, 2762, 2833
BamHI GGATCC 1 cut(s) 2085
BanI GGYRCC 2 cut(s) 313, 4410
BanII GRGCYC 2 cut(s) 138, 3744
BarI GAAGNNNNNNTAC 2 cut(s) 513, 545
BauI CACGAG 1 cut(s) 2153
BbsI GAAGAC 5 cut(s) 81, 528, 2357, 2577, 2598
Bbv12I GWGCWC 1 cut(s) 2848
BcgI CGANNNNNNTGC 4 cut(s) 554, 588, 3915, 3949
BciT130I CCWGG 1 cut(s) 3867
BciVI GTATCC 2 cut(s) 1969, 2190
BclI TGATCA 2 cut(s) 1944, 4603
BcoDI GTCTC 4 cut(s) 1661, 2024, 3153, 3876
BcuI ACTAGT 1 cut(s) 3404
BfaI CTAG 7 cut(s) 936, 2349, 2661, 3083, 3405, 4079, 4638
BfmI CTRYAG 3 cut(s) 1158, 3650, 3947
BfoI RGCGCY 3 cut(s) 212, 1627, 1829
BfrI CTTAAG 1 cut(s) 4025
BfuAI ACCTGC 1 cut(s) 3568
BfuI GTATCC 2 cut(s) 1969, 2190
BglI GCCNNNNNGGC 1 cut(s) 1115
BglII AGATCT 4 cut(s) 276, 1096, 3010, 4219
Bme1390I CCNGG 1 cut(s) 3867
Bme18I GGWCC 3 cut(s) 271, 2271, 4106
BmeT110I CYCGRG 2 cut(s) 131, 737
BmgT120I GGNCC 7 cut(s) 271, 779, 2271, 2789, 3740, 3741, 4106
BmiI GGNNCC 5 cut(s) 302, 315, 2087, 3742, 4412
BmrFI CCNGG 1 cut(s) 3867
BmrI ACTGGG 1 cut(s) 4411
BmuI ACTGGG 1 cut(s) 4411
BpiI GAAGAC 5 cut(s) 81, 528, 2357, 2577, 2598
BpmI CTGGAG 2 cut(s) 1791, 3337
Bpu14I TTCGAA 2 cut(s) 2983, 3778
BpuEI CTTGAG 2 cut(s) 1996, 3161
Bsa29I ATCGAT 1 cut(s) 3621
BsaAI YACGTR 2 cut(s) 83, 1344
BsaBI GATNNNNATC 1 cut(s) 545
BsaHI GRCGYC 1 cut(s) 4530
BsaI GGTCTC 2 cut(s) 3153, 3876
BsaJI CCNNGG 6 cut(s) 656, 1890, 3540, 3700, 3866, 4490
BsaWI WCCGGW 4 cut(s) 29, 1297, 1551, 4361
Bsc4I CCNNNNNNNGG 4 cut(s) 280, 310, 444, 4246
Bse8I GATNNNNATC 1 cut(s) 545
BseAI TCCGGA 1 cut(s) 29
BseBI CCWGG 1 cut(s) 3867
BseCI ATCGAT 1 cut(s) 3621
BseDI CCNNGG 6 cut(s) 656, 1890, 3540, 3700, 3866, 4490
BseJI GATNNNNATC 1 cut(s) 545
BseLI CCNNNNNNNGG 4 cut(s) 280, 310, 444, 4246
BseMII CTCAG 2 cut(s) 4211, 4476
BseRI GAGGAG 2 cut(s) 1573, 3334
BseSI GKGCMC 5 cut(s) 318, 2848, 3744, 4382, 4415
BseYI CCCAGC 3 cut(s) 468, 498, 2144
BsgI GTGCAG 4 cut(s) 702, 1040, 1147, 3102
BshFI GGCC 8 cut(s) 781, 900, 1052, 1118, 2762, 2790, 2833, 3742
BshNI GGYRCC 2 cut(s) 313, 4410
BshVI ATCGAT 1 cut(s) 3621
BsiHKAI GWGCWC 1 cut(s) 2848
BsiHKCI CYCGRG 2 cut(s) 131, 737
BsiSI CCGG 4 cut(s) 30, 1298, 1552, 4362
BslFI GGGAC 1 cut(s) 814
BslI CCNNNNNNNGG 4 cut(s) 280, 310, 444, 4246
BsmAI GTCTC 4 cut(s) 1661, 2024, 3153, 3876
BsmFI GGGAC 1 cut(s) 814
BsmI GAATGC 1 cut(s) 577
BsnI GGCC 8 cut(s) 781, 900, 1052, 1118, 2762, 2790, 2833, 3742
Bso31I GGTCTC 2 cut(s) 3153, 3876
BsoBI CYCGRG 2 cut(s) 131, 737
Bsp119I TTCGAA 2 cut(s) 2983, 3778
Bsp120I GGGCCC 1 cut(s) 3740
Bsp1286I GDGCHC 6 cut(s) 138, 318, 2848, 3744, 4382, 4415
Bsp13I TCCGGA 1 cut(s) 29
Bsp1407I TGTACA 3 cut(s) 1072, 1725, 2803
Bsp19I CCATGG 1 cut(s) 1890
BspACI CCGC 4 cut(s) 298, 544, 1010, 3362
BspANI GGCC 8 cut(s) 781, 900, 1052, 1118, 2762, 2790, 2833, 3742
BspCNI CTCAG 2 cut(s) 4210, 4477
BspDI ATCGAT 1 cut(s) 3621
BspEI TCCGGA 1 cut(s) 29
BspHI TCATGA 1 cut(s) 4606
BspLI GGNNCC 5 cut(s) 302, 315, 2087, 3742, 4412
BspMAI CTGCAG 1 cut(s) 3951
BspMI ACCTGC 1 cut(s) 3568
BspPI GGATC 7 cut(s) 329, 535, 811, 1001, 2040, 2080, 2093
BspQI GCTCTTC 1 cut(s) 147
BspT104I TTCGAA 2 cut(s) 2983, 3778
BspT107I GGYRCC 2 cut(s) 313, 4410
BspTI CTTAAG 1 cut(s) 4025
BspTNI GGTCTC 2 cut(s) 3153, 3876
BsrGI TGTACA 3 cut(s) 1072, 1725, 2803
BssECI CCNNGG 6 cut(s) 656, 1890, 3540, 3700, 3866, 4490
BssNI GRCGYC 1 cut(s) 4530
BssSI CACGAG 1 cut(s) 2153
BssT1I CCWWGG 4 cut(s) 1890, 3540, 3700, 4490
Bst2BI CACGAG 1 cut(s) 2153
Bst2UI CCWGG 1 cut(s) 3867
Bst6I CTCTTC 8 cut(s) 147, 411, 1206, 1395, 1550, 2898, 3978, 4184
BstACI GRCGYC 1 cut(s) 4530
BstAFI CTTAAG 1 cut(s) 4025
BstAPI GCANNNNNTGC 2 cut(s) 368, 1018
BstAUI TGTACA 3 cut(s) 1072, 1725, 2803
BstBAI YACGTR 2 cut(s) 83, 1344
BstBI TTCGAA 2 cut(s) 2983, 3778
BstC8I GCNNGC 6 cut(s) 353, 454, 1413, 2702, 2894, 4267
BstDSI CCRYGG 2 cut(s) 656, 1890
BstH2I RGCGCY 3 cut(s) 212, 1627, 1829
BstHHI GCGC 3 cut(s) 211, 1626, 1828
BstMAI GTCTC 4 cut(s) 1661, 2024, 3153, 3876
BstMWI GCNNNNNNNGC 7 cut(s) 368, 1018, 1115, 2381, 4118, 4193, 4336
BstNI CCWGG 1 cut(s) 3867
BstNSI RCATGY 5 cut(s) 974, 1415, 1904, 3298, 3470
BstSCI CCNGG 1 cut(s) 3865
BstSFI CTRYAG 3 cut(s) 1158, 3650, 3947
BstSLI GKGCMC 5 cut(s) 318, 2848, 3744, 4382, 4415
BstV2I GAAGAC 5 cut(s) 81, 528, 2357, 2577, 2598
BstX2I RGATCY 7 cut(s) 276, 334, 1096, 2045, 2085, 3010, 4219
BstXI CCANNNNNNTGG 1 cut(s) 4594
BstYI RGATCY 7 cut(s) 276, 334, 1096, 2045, 2085, 3010, 4219
Bsu15I ATCGAT 1 cut(s) 3621
BsuI GTATCC 2 cut(s) 1969, 2190
BsuRI GGCC 8 cut(s) 781, 900, 1052, 1118, 2762, 2790, 2833, 3742
BsuTUI ATCGAT 1 cut(s) 3621
BtgI CCRYGG 2 cut(s) 656, 1890
BtsI GCAGTG 2 cut(s) 1031, 3305
BveI ACCTGC 1 cut(s) 3568
Cac8I GCNNGC 6 cut(s) 353, 454, 1413, 2702, 2894, 4267
CciI TCATGA 1 cut(s) 4606
CfoI GCGC 3 cut(s) 211, 1626, 1828
Cfr13I GGNCC 7 cut(s) 271, 779, 2271, 2789, 3740, 3741, 4106
ClaI ATCGAT 1 cut(s) 3621
CseI GACGC 1 cut(s) 4519
DraI TTTAAA 2 cut(s) 1641, 3420
EaeI YGGCCR 4 cut(s) 898, 1050, 2760, 2831
Eam1104I CTCTTC 8 cut(s) 147, 411, 1206, 1395, 1550, 2898, 3978, 4184
EarI CTCTTC 8 cut(s) 147, 411, 1206, 1395, 1550, 2898, 3978, 4184
EciI GGCGGA 1 cut(s) 313
Eco130I CCWWGG 4 cut(s) 1890, 3540, 3700, 4490
Eco24I GRGCYC 2 cut(s) 138, 3744
Eco31I GGTCTC 2 cut(s) 3153, 3876
Eco32I GATATC 1 cut(s) 2581
Eco47I GGWCC 3 cut(s) 271, 2271, 4106
Eco47III AGCGCT 1 cut(s) 1625
Eco57I CTGAAG 7 cut(s) 804, 2490, 2802, 2922, 3276, 4121, 4328
Eco88I CYCGRG 2 cut(s) 131, 737
EcoRI GAATTC 3 cut(s) 906, 1506, 2139
EcoRII CCWGG 1 cut(s) 3865
EcoRV GATATC 1 cut(s) 2581
EcoT14I CCWWGG 4 cut(s) 1890, 3540, 3700, 4490
EcoT22I ATGCAT 3 cut(s) 577, 2649, 2742
EcoT38I GRGCYC 2 cut(s) 138, 3744
ErhI CCWWGG 4 cut(s) 1890, 3540, 3700, 4490
FalI AAGNNNNNCTT 2 cut(s) 2528, 2560
FaqI GGGAC 1 cut(s) 814
FbaI TGATCA 2 cut(s) 1944, 4603
FblI GTMKAC 2 cut(s) 408, 1860
FriOI GRGCYC 2 cut(s) 138, 3744
FspBI CTAG 7 cut(s) 936, 2349, 2661, 3083, 3405, 4079, 4638
GlaI GCGC 3 cut(s) 210, 1625, 1827
GsaI CCCAGC 3 cut(s) 472, 502, 2148
GsuI CTGGAG 2 cut(s) 1791, 3337
HaeII RGCGCY 3 cut(s) 212, 1627, 1829
HaeIII GGCC 8 cut(s) 781, 900, 1052, 1118, 2762, 2790, 2833, 3742
HapII CCGG 4 cut(s) 30, 1298, 1552, 4362
HgaI GACGC 1 cut(s) 4519
HhaI GCGC 3 cut(s) 211, 1626, 1828
Hin1I GRCGYC 1 cut(s) 4530
Hin6I GCGC 3 cut(s) 209, 1624, 1826
HinP1I GCGC 3 cut(s) 209, 1624, 1826
HincII GTYRAC 3 cut(s) 409, 931, 3402
HindII GTYRAC 3 cut(s) 409, 931, 3402
HindIII AAGCTT 2 cut(s) 375, 2894
HpaI GTTAAC 1 cut(s) 3402
HpaII CCGG 4 cut(s) 30, 1298, 1552, 4362
Hpy99I CGWCG 3 cut(s) 53, 173, 2442
HpyCH4IV ACGT 6 cut(s) 51, 82, 755, 942, 1343, 1815
HpyF10VI GCNNNNNNNGC 7 cut(s) 368, 1018, 1115, 2381, 4118, 4193, 4336
HpySE526I ACGT 6 cut(s) 51, 82, 755, 942, 1343, 1815
Hsp92I GRCGYC 1 cut(s) 4530
HspAI GCGC 3 cut(s) 209, 1624, 1826
Kpn2I TCCGGA 1 cut(s) 29
Ksp22I TGATCA 2 cut(s) 1944, 4603
KspAI GTTAAC 1 cut(s) 3402
LguI GCTCTTC 1 cut(s) 147
LmnI GCTCC 3 cut(s) 300, 4117, 4631
MaeI CTAG 7 cut(s) 936, 2349, 2661, 3083, 3405, 4079, 4638
MaeII ACGT 6 cut(s) 51, 82, 755, 942, 1343, 1815
MaeIII GTNAC 9 cut(s) 938, 1262, 1274, 1499, 1843, 2635, 3033, 3628, 3936
MfeI CAATTG 5 cut(s) 795, 816, 1395, 3941, 4211
MflI RGATCY 7 cut(s) 276, 334, 1096, 2045, 2085, 3010, 4219
MhlI GDGCHC 6 cut(s) 138, 318, 2848, 3744, 4382, 4415
MlsI TGGCCA 4 cut(s) 900, 1052, 2762, 2833
MluNI TGGCCA 4 cut(s) 900, 1052, 2762, 2833
MlyI GAGTC 2 cut(s) 1412, 3574
MmeI TCCRAC 3 cut(s) 1858, 3198, 4484
Mox20I TGGCCA 4 cut(s) 900, 1052, 2762, 2833
Mph1103I ATGCAT 3 cut(s) 577, 2649, 2742
MroI TCCGGA 1 cut(s) 29
MroXI GAANNNNTTC 1 cut(s) 3434
MscI TGGCCA 4 cut(s) 900, 1052, 2762, 2833
MslI CAYNNNNRTG 8 cut(s) 85, 1283, 1905, 2642, 3686, 3824, 3909, 4511
Msp20I TGGCCA 4 cut(s) 900, 1052, 2762, 2833
MspA1I CMGCKG 3 cut(s) 1214, 2148, 4187
MspCI CTTAAG 1 cut(s) 4025
MspI CCGG 4 cut(s) 30, 1298, 1552, 4362
MspR9I CCNGG 1 cut(s) 3867
MunI CAATTG 5 cut(s) 795, 816, 1395, 3941, 4211
Mva1269I GAATGC 1 cut(s) 577
MvaI CCWGG 1 cut(s) 3867
MwoI GCNNNNNNNGC 7 cut(s) 368, 1018, 1115, 2381, 4118, 4193, 4336
NcoI CCATGG 1 cut(s) 1890
NlaIV GGNNCC 5 cut(s) 302, 315, 2087, 3742, 4412
NmeAIII GCCGAG 1 cut(s) 185
NmuCI GTSAC 6 cut(s) 938, 1274, 1843, 2635, 3628, 3936
NsiI ATGCAT 3 cut(s) 577, 2649, 2742
NspI RCATGY 5 cut(s) 974, 1415, 1904, 3298, 3470
NspV TTCGAA 2 cut(s) 2983, 3778
OliI CACNNNNGTG 1 cut(s) 85
PaeI GCATGC 1 cut(s) 1415
PaeR7I CTCGAG 2 cut(s) 131, 737
PagI TCATGA 1 cut(s) 4606
PaqCI CACCTGC 1 cut(s) 3568
PciI ACATGT 2 cut(s) 1900, 3466
PciSI GCTCTTC 1 cut(s) 147
PcsI WCGNNNNNNNCGW 1 cut(s) 212
PctI GAATGC 1 cut(s) 577
PdmI GAANNNNTTC 1 cut(s) 3434
PflMI CCANNNNNTGG 2 cut(s) 280, 310
PleI GAGTC 2 cut(s) 1411, 3574
PpsI GAGTC 2 cut(s) 1411, 3574
Ppu21I YACGTR 2 cut(s) 83, 1344
PscI ACATGT 2 cut(s) 1900, 3466
PsiI TTATAA 2 cut(s) 1524, 2093
Psp1406I AACGTT 1 cut(s) 755
Psp6I CCWGG 1 cut(s) 3865
PspFI CCCAGC 3 cut(s) 468, 498, 2144
PspGI CCWGG 1 cut(s) 3865
PspN4I GGNNCC 5 cut(s) 302, 315, 2087, 3742, 4412
PspOMI GGGCCC 1 cut(s) 3740
PspPI GGNCC 7 cut(s) 271, 779, 2271, 2789, 3740, 3741, 4106
PspXI VCTCGAGB 1 cut(s) 131
PstI CTGCAG 1 cut(s) 3951
PsuI RGATCY 7 cut(s) 276, 334, 1096, 2045, 2085, 3010, 4219
PvuII CAGCTG 3 cut(s) 1214, 2148, 4187
RseI CAYNNNNRTG 8 cut(s) 85, 1283, 1905, 2642, 3686, 3824, 3909, 4511
SalI GTCGAC 1 cut(s) 407
SapI GCTCTTC 1 cut(s) 147
Sau96I GGNCC 7 cut(s) 271, 779, 2271, 2789, 3740, 3741, 4106
SchI GAGTC 2 cut(s) 1412, 3574
ScrFI CCNGG 1 cut(s) 3867
SduI GDGCHC 6 cut(s) 138, 318, 2848, 3744, 4382, 4415
SfcI CTRYAG 3 cut(s) 1158, 3650, 3947
Sfr274I CTCGAG 2 cut(s) 131, 737
SfuI TTCGAA 2 cut(s) 2983, 3778
SinI GGWCC 3 cut(s) 271, 2271, 4106
SlaI CTCGAG 2 cut(s) 131, 737
SmiMI CAYNNNNRTG 8 cut(s) 85, 1283, 1905, 2642, 3686, 3824, 3909, 4511
SmlI CTYRAG 5 cut(s) 131, 737, 1975, 3140, 4025
SmoI CTYRAG 5 cut(s) 131, 737, 1975, 3140, 4025
SpeI ACTAGT 1 cut(s) 3404
SphI GCATGC 1 cut(s) 1415
SsiI CCGC 4 cut(s) 298, 544, 1010, 3362
SspI AATATT 3 cut(s) 1711, 3382, 3725
SspMI CTAG 7 cut(s) 936, 2349, 2661, 3083, 3405, 4079, 4638
StyD4I CCNGG 1 cut(s) 3865
StyI CCWWGG 4 cut(s) 1890, 3540, 3700, 4490
TaiI ACGT 6 cut(s) 54, 85, 758, 945, 1346, 1818
TaqII GACCGA 3 cut(s) 1443, 1703, 2144
TatI WGTACW 6 cut(s) 1072, 1725, 1735, 2803, 3302, 3654
TseFI GTSAC 6 cut(s) 938, 1274, 1843, 2635, 3628, 3936
Tsp45I GTSAC 6 cut(s) 938, 1274, 1843, 2635, 3628, 3936
TspGWI ACGGA 4 cut(s) 645, 1445, 1878, 2739
Van91I CCANNNNNTGG 2 cut(s) 280, 310
Vha464I CTTAAG 1 cut(s) 4025
VneI GTGCAC 1 cut(s) 2844
VpaK11BI GGWCC 3 cut(s) 271, 2271, 4106
XbaI TCTAGA 1 cut(s) 3082
XceI RCATGY 5 cut(s) 974, 1415, 1904, 3298, 3470
XhoI CTCGAG 2 cut(s) 131, 737
XmiI GTMKAC 2 cut(s) 408, 1860
XmnI GAANNNNTTC 1 cut(s) 3434
XspI CTAG 7 cut(s) 936, 2349, 2661, 3083, 3405, 4079, 4638
Zsp2I ATGCAT 3 cut(s) 577, 2649, 2742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.