RchiOBHm_Chr5g0080281

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000085
Physical Location & Seq
Reverse (-)
86192490 .. 86202828
10339 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35461

Sequence Viewer

Length: 6291 bp
ATGGATTCTCAGGAAGTCACTACTAAAGTCCGATTAGTCAAATGTCCTAAATGTCGGCTGCTTCTACCAGAGTTGCCGGATTCTCAGGTTTACGAGTGTGGTGGATGTGGTGCCATTCTCCAAGCAAAAAATCGATTAAGAAATGAGAAAAGAAGTGAGGGCTTAACCATTGAATCAGCTCCAGTGAATCAATTATTAGATCATTTTTCTCGAGATTCAGAAATGGTCAGCTCAGATGTGGCCTGGTTCTCTCGAAAAGCTGATGCTTCTCTTCCTTCATCAGCTGAACTATCATCAGCAGCTGGTCGGTGGAAGTATGATGTGTATTTGAGTTTCAGGGGTGTAGATACTCGAAGGGGTTTTACATCCCATTTATACGATCGGCTGCAGAGGAGTGGAATTAAGACATTCATGGATGATCAAGATCTTCAAGTAGGGGATGATGTTTCTCCCACTCTCCTAAAGGCAATTGAAGGATCAAGGTTCGCAATTGTTGTTCTGTCTCAAAATTATGCTTCTTCTGCTTGGTGTTTGGATGAACTTACAAAGATTTGTCAGTTCATGGAGGACGGCCCTAGAATTCTTCCAGTTTTTTATGAGGTTGGCCCTAGTCATGTAAGAAAACAAAAGGGGAGTTTTGAGGATGCTTTAGCCAAGCATGAAAGGTCTGGGCGATACGAATCGAAAAAGGTCCAGCAGTGGAGAGATGCTTTAAGAAAAGTGGCCGACTTTGCTGGGTTTGATACACAGAATTACAGGACTGATATAGATCTTCTCGAACACATTGTGGAAATTGTGTGCAGTAAAGTACAATCTGTTTTGGAAATTGAAATAGTACAACCTATTGAAATCAACACAGGAGGTTTTGAATTATTTGAACCAACAAGAAAAGCCATGGATGACGTTATGGAGGCGCTAAAAGATGACGAGGTCACTGCCGTTGGTGTCTACGGCATGGCAGGCGTTGGAAAGACAGCCATGGTAAAACATGTCGGTGCACATGCCCGAAAAAGTGGAATTTTTCATCATGTGATTATCGGTGATGTATCCCAAAGCCCTGACTTGGAAACAATTCAAGGCACATTGGCAGGGCAGCTGGGCTACAAATTACATGAGAAGACAGAAATTGGCAGAGCAGCTAGATTGAGTGAGGAGATAAAGAGAAGGGGAAAGATCCTTATAATCCTGGACGACATTTGGGAGAGAATGGATTTGTCAGACATAGGAATACCTAGCTATAAGGAGCTTCAAAAGTGCAATTCCAAAGTCCTACTCACCACACGGAGAGAGAATGTGTGTTATGAGATGAGATGCCAAGCAATTATTCATCTCAATATCTTATCAGAACAAGATTCTTGGGCACTTGGAGGTAAAGAACTAGTGGAATGGAAAAGAGCAGCTCGAGCACTAGAGAAGTCGCAATCTGCCAATCCTGAACATGAAGGAGAGGCATTCAGATGTATAAAATTAAGCTATGATTACTTGAAAGATGAAGATACCAAGTCATGCTTCTTGCTTTGTTGCCTATTTCCTGAAGACTATGACATCCCAATAGGAGACTTGTTCAAGTATGCAATCGGGAAAGGATTGTTTCGGGATGCCGAGACAATCCAAGAAGCTAGAGCATCAGCTGATTCAGCCGTAAGGTACCTGAAACATTTTAGCTTGCTTTTGGATAGTGAGAAAAAGGGGTGTGTAAGGATGCAGGATGTCATTCGGGATGCTGCCCTGAATATTGCAAAATCTGAAGACGGGCATGGGTTTTTGGTAAAAGCTGGGTGTGGTTTAAAAAGCTGGCCACATCAATTACATGAAGGCTACTCTGCAATCTCATTAATGAACAATGATATCCACAAGCTACCCGGAGAGCTGGTATGTCCAAAACTCCAGATTTTATTACTACAGCAGAATGCTGATTTAAATGAGATGCCAGGAACTTTTTTCCAAAGTCCTAATGAATTAAGGGTCTTAGACCTTAGCCTCACCAGCATTACAGAACTTCCTCAATCATTCAGTCTCCTAATGAACCTCCAAGCTTTATATCTAGATTTTTGCCAGAGACTGATTGACTTTTCCTTAATTGGAAAACTAAAGAAGCTTGAGATTCTTAGTATGAGAGGAAATGTTTTGAAAGAATTGTCGAGAGAAATAGGGAATCTGATCAATCTAAGGATGCTGGACATTACTGATGGAATTATTAGCAAAATTCCATCAAAAGTGATATCGCAGTTGCATAGATTAGAAGAACTCTACATGCCATTTCGATTTGGGAATTGGGTCAGGAAGGTTGAAGGAGAAGGAGAAGAAACCGATATTGGCTTTGGTGAGGTAACTGGCTTAACATGTCTAAATATTTTGAAGGTTTCCCTACCTGCAAAATGCATCCCTGAAGAGCTTGAGTTTTATCCCAATTGGTTGTTCTTTGATATTTGTATTAGTGGGGACACAAGCAGCAGAGACATTACGAGAGACATAAGTAATCAAGGCTTTCAACACAGTCATAAAGATTCAAGATCCTTGAGTCTTGACACAAGAATTAGTGCCTTACCAGCTTGGTTTGTCCATGAGGTAACAGTCAAGACAGAGAAGCTACTGTACGTAAACTGCAAATGGTTAAATAACATTCTTGTTGAATATCACCATGGGAGGTTACATATGCTGAAACATCTCTCTATTATTGGTCCCAGTGAGAGCTTGGAAGAGCTGATGAATGCTATAACATGGGTTCCAGTTAAACCCGTGTTCGAGAACTTGGAAGAGTTGCATTTGGAGTGTGTGACTTCTTTGAAAGAGTTATGTGTTGGAAAATTACCACCTGGGTCTCTATACAATCTGAAGTTATTGAAGGTCCATTATTGTCCTAACTTGGGGAATATTCTTTTACCATCAAAATTGTTACATAGATTGCCAAATCTGGAGAACATAATCTGTAGGGATATGTCTAGACTGGAACATGTGTTTGGATGTCAAGGGTTTGAGCCAGAGCAATCAAAACTGAGAGAGATGACCTTTATCAATCTACCTGCTATAAGAAACATATGTAACGGTCCAGGTCCAAGTGCAATGTTCCAAGCTCTTAAAAGTTTGTTCATTTGTCATTGCAACTTTCTAGGATGTCTCTTCTCGTTTGATACTGCTCAAGTTCTTTTTCAATTGGAAGACTTTTTTGTAGACAGTTGCCCTTTATTGGAAAGACTAATTGAAGTACGCACGGAAAGAGTGAACAACAAGAAGATCATTCTTCCAAAATTGAAGAACTTGGGTTTAGAGAAACTTCCTATATTGTACAGTGGAGGTGCTACTATTGATATCGAGTGTCCTTCCTTGGAACACTTGTATGTGAAGGAGTGCCCCCAGTTTACAGCCTCTGCTTCTGACTTCCAGAGCAAAAACCAAGTCCAAGTCAACGATAAATTACATTACTTTGGTCTACTTGGCAGAAGGACTGAAGGACAACATATTGAAGCCATTGCGGAATCTGTGTTCAGTACATTACGACCTATTGAAACTGAGTTTACAATGCCCATGGGAGATTTTCAAGCATTTGAAGCAACAAGACAGGCCATGAATAAGGTTATGAAGGCACTAGAAGCTGATGAGGTCACAGCCATTGGGGTCTACGGAATGGGAGGAGTTGGAAAATCAACCATGGTTGAACATGTTTGTGCAGAAGTTCGGGATAATGGGATGTTTCATCAGGTGATTTCAGCTGTTGTATCCCAAAACCCTGACTTGAGAAAAATTCAAGGCACATTGGCAGATATGTTGGGCTTTATATTGGAGGAGGAGACAGAAATTGGAAGAGCTCGTAGATTGATGCAGAGGATAAGGAGAGGAAATAGGATCCTTGTAATCTTGGACAACATTTGGGAGAGAACAGATTTGTCAAATATAGGAATTCCCAGCTACTACGAGCTTCAAAGATGCAATTCCAAAGTCCTACTCATCACAAGGACACAGAATGTCTGTCATGCCATGGAATGCCAATCAAACATTTCTCTTGATGTCTTATCAGAAGAAGATTCTTGGACCTTATTTGTGAACAAAGCAAGAACGTCTTTTGAATCGACCAGTTTGTGTGATGTTGCAAAGAAGGTAGCTAGAGTATGTGCTGGTCTTCCAGTTGCGTTGATAGCAGTTGCGAAGGCACTTGGTGATAAGTGTTTGGATGACTGGAAGGAAGCGGCTCGACGACTAGAGGCTTCTCAACCTGCCAGCCTTGAAGATGGGAAAGTTGTGTTCAATTGTATAAAATTAAGCTATGATTACTTGATATCTGATGATGCCAGGTCATGCTTCTTGCTTTGTTGCCTCTTCCCAGAAGACTATGATATCCAAATTGAAGAGTTGTTAAAGTATGGGATGGGGAAAGGATTGTTTCAAGATTCCTGCACAATGCAAGATGCCAGAGTAACTGCACATTTGGTGGTCAAGTACCTTAAAGACTGTAGCTTGCTTTTGGAAACTGAACAGGACGGATGTGTAAAGATGCATGATGTCATTCGGGATGTGGCCATAAGGATTTCGTTGTCTGAGGATGGCCCACTGTTTTTTGTACAAGCTGGTTGTCAATTAAAGGAATGGCCAAGTATTAGTGCACATGAAGGCTACTCTGCTATTTCACTAATTATGAATAAAATTTGCAAGCTTCCTGAAGAGTTGGTATGTCCGAAACTCCTTATTTTGTTTCTGCAAAGTAATGTTTGGTTGAATGAGATCCCAGAATATTTCTTTCAAAGTCCGAATGTGTTGAGGGTATTAGATCTAAGCCGAACCAGAATTTCTTTTCTACCCACATCATTCAATATCTTGATCAACCTCCAGACCTTGTATCTAGATGGTTGCTTGTCAACCATAGATAACATATCCATACTCGGAAAATTGAAAAAACTTGAGATTCTTAGTATGAGAGAACTTCCTCTTGTGGAACTGCCAAAAGAAATAGGAGATTTGAGCCATCTAAGGATGTTAGACATCACTGGTGATTATGTTGATAATGTTCCGTCTGAAGTGATATGCAACCTTAATTTGTTAGAGGAACTATACATGCAGTGTAATTTTGCAGACTGGGGGAGTAAAGTTGAGGGAGCAGGAGAAGAAAATATTGTTGCGTTTGATGAGTTAACTGGCTTGTCATGTCTAAATGTTTTGAAGGTTTTCATATCTGATGCTAAATGTTTGCCTAAACATGTGGAGGTTGAACCAAATTGGGTAAACTTTGATATTTGTATCAGCAGAGACCCATCCATCAGAAAAGTGTCGTTGGTTTCAAGTTCTTCACCCGCTTTTACAAGAGCCTTGACTCTTGATACAACCATCAATGCCTTACCAGGTTGGTTTATCAATGCGGTGACAATGAAAGCAGAGAAACTGCAGTATATAATGTGTTGGGGATTAGATAACATTCTTGTGGAATATGACTGTGGGAGATTACATGCACTGAAGTATTTATCTGTTATTGGCCCCAATGAGAACTTGAAAATGTTGATGAACACAGTAACACGCTTTTGGAATGAACCAGTGTTTCAGAACTTGGAAGAGTTGCATCTCGTTCAAGTGGATTGCCTGAAGGAGTTATGTGTTGGTGAATTGCCACCTGGGTCTCTATGCAATCTTAGGTTACTGAAGGTGAAACATTGCTATGAATTGGTGGATGCACTCCTACCGTCACGGTTGCTGCAGAGATTACAAAATCTGGAAGAACTGATTTGTGAAGAGATAGATGAATTGGAATATGTTTTTGGATGTGAAGGGCTTGAGCCAGAACAAATGATATTGACACAATTGATAGAAATGAGATTGGAGAATCTAGGGAAACTAATAAAGATATGGAATGGTCCTGCTCATTGTGCAATCTTCCAGAATCTTCAGAGTTTGGTAGTTTCCAGGTGCTTTGAACTGGAAAATCTCTTCACAGCTGATGTAGCTCAATGTCTTTTGCACTTGGAAGATCTTTCAGTGGAGCTTTGCCCAATGTTGGACAATATTATTGAAGCAAGTAAGGACACAGTGGACAACAAGATTGTTTTTCCAGAATTGAAGAACTTAACTTTAATAAAGCTTCCACAACTCACAAGGTTCTGCAGCAGTACAGGCAGTGCTATTGAGTGCCCTTTACTGGAATACTTGTATGTGGAGAGGTGCCCCCAGTTTTCAACGTCTGACTTCCATAGCAGGAACAAAGTCGAACCCAAACATCCACAACATTTGGACATTGTATTGAGAAGGGCCAGAGCAGAGAAAAGAAGAAAAATCAAATTCACAGAGGTCGTCCAAGAAGACAGTAAGTAG

Protein Analysis

2096

Amino Acids

237.2

Weight (kDa)

5.49

Isoelectric Point (pI)

47.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR4-like_1st PF22910 9 - 42 1.5e-17 Enhanced disease resistance 4-like, N-terminal domain
TIR PF01582 105 - 273 3.2e-50 TIR domain
TIR_2 PF13676 108 - 202 8.6e-17 TIR domain
NB-ARC PF00931 297 - 456 7.1e-27 NB-ARC domain
LRR_8 PF13855 627 - 686 3.5e-08 Leucine rich repeat
LRR_14 PF23598 689 - 753 3.2e-07 Leucine-rich repeat region
LRR_RPS2 PF23247 918 - 1060 2.2e-20 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1014 - 1121 4e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
NB-ARC PF00931 1187 - 1350 2.2e-28 NB-ARC domain
LRR_14 PF23598 1610 - 1685 1.5e-09 Leucine-rich repeat region
LRR_RPS2 PF23247 1845 - 1990 6.3e-28 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1181
Acc36I ACCTGC 3 cut(s) 2380, 3031, 4219
Acc65I GGTACC 1 cut(s) 1647
AccB1I GGYRCC 3 cut(s) 110, 1647, 6141
AccI GTMKAC 4 cut(s) 948, 3171, 3430, 3618
AciI CCGC 4 cut(s) 3473, 4184, 5283, 5348
AclWI GGATC 6 cut(s) 484, 1168, 2508, 3838, 3851, 4711
AcoI YGGCCR 4 cut(s) 723, 1796, 4512, 4583
AcsI RAATTY 8 cut(s) 579, 1017, 2205, 3741, 3897, 4638, 4779, 6257
AdeI CACNNNGTG 2 cut(s) 787, 4154
AfiI CCNNNNNNNGG 5 cut(s) 1063, 2867, 3187, 5977, 6118
AflIII ACRYGT 5 cut(s) 988, 2342, 2953, 3658, 5188
AhlI ACTAGT 1 cut(s) 1378
AjnI CCWGG 9 cut(s) 242, 1185, 1930, 2815, 3049, 4286, 5329, 5596, 5885
AloI GAACNNNNNNTCC 2 cut(s) 3467, 3499
Alw21I GWGCWC 4 cut(s) 1000, 1408, 3808, 4600
Alw44I GTGCAC 2 cut(s) 996, 4596
AlwI GGATC 6 cut(s) 484, 1168, 2508, 3838, 3851, 4711
AlwNI CAGNNNCTG 3 cut(s) 302, 2062, 3368
Ama87I CYCGRG 2 cut(s) 210, 1401
ApaLI GTGCAC 2 cut(s) 996, 4596
ApoI RAATTY 8 cut(s) 579, 1017, 2205, 3741, 3897, 4638, 4779, 6257
ArsI GACNNNNNNTTYG 2 cut(s) 3381, 3413
AseI ATTAAT 1 cut(s) 1835
Asp700I GAANNNNTTC 2 cut(s) 1071, 5156
Asp718I GGTACC 1 cut(s) 1647
AspLEI GCGC 1 cut(s) 916
AsuC2I CCSGG 1 cut(s) 1863
AvaI CYCGRG 2 cut(s) 210, 1401
AvaII GGWCC 7 cut(s) 691, 2681, 2848, 3047, 3053, 4029, 5837
BaeGI GKGCMC 6 cut(s) 1000, 1363, 3353, 4600, 6113, 6146
BalI TGGCCA 3 cut(s) 1798, 4514, 4585
BamHI GGATCC 1 cut(s) 3843
BanI GGYRCC 3 cut(s) 110, 1647, 6141
BanII GRGCYC 1 cut(s) 3808
BarI GAAGNNNNNNTAC 4 cut(s) 346, 378, 2579, 2611
BbsI GAAGAC 7 cut(s) 1124, 1542, 1755, 3165, 4109, 4329, 6285
Bbv12I GWGCWC 4 cut(s) 1000, 1408, 3808, 4600
BceAI ACGGC 4 cut(s) 586, 923, 967, 1625
BcgI CGANNNNNNTGC 2 cut(s) 917, 951
BciT130I CCWGG 9 cut(s) 244, 1187, 1932, 2817, 3051, 4288, 5331, 5598, 5887
BciVI GTATCC 2 cut(s) 1057, 3727
BclI TGATCA 3 cut(s) 418, 2160, 4812
BcnI CCSGG 1 cut(s) 1863
BcuI ACTAGT 1 cut(s) 1378
BfmI CTRYAG 7 cut(s) 386, 1901, 2929, 4447, 5372, 5678, 6082
BfoI RGCGCY 1 cut(s) 917
BfuAI ACCTGC 3 cut(s) 2380, 3031, 4219
BfuI GTATCC 2 cut(s) 1057, 3727
BglII AGATCT 4 cut(s) 424, 769, 4762, 5950
Bme18I GGWCC 7 cut(s) 691, 2681, 2848, 3047, 3053, 4029, 5837
BmeT110I CYCGRG 2 cut(s) 210, 1401
BmiI GGNNCC 7 cut(s) 112, 1649, 2683, 2727, 3845, 5464, 6143
BmrI ACTGGG 4 cut(s) 2679, 3349, 5077, 6142
BmuI ACTGGG 4 cut(s) 2679, 3349, 5077, 6142
BpiI GAAGAC 7 cut(s) 1124, 1542, 1755, 3165, 4109, 4329, 6285
BpmI CTGGAG 4 cut(s) 165, 1871, 2936, 4805
Bpu10I CCTNAGC 1 cut(s) 1976
BpuEI CTTGAG 7 cut(s) 2120, 2417, 2539, 3123, 3754, 4913, 5777
BpuMI CCSGG 1 cut(s) 1863
Bsa29I ATCGAT 1 cut(s) 133
BsaAI YACGTR 1 cut(s) 2599
BsaBI GATNNNNATC 3 cut(s) 423, 679, 768
BsaI GGTCTC 3 cut(s) 2826, 5232, 5607
BsaJI CCNNGG 9 cut(s) 894, 978, 2641, 2816, 3324, 3525, 3648, 3975, 5597
BsaXI ACNNNNNCTCC 2 cut(s) 1857, 1887
Bsc4I CCNNNNNNNGG 5 cut(s) 1063, 2867, 3187, 5977, 6118
Bse3DI GCAATG 4 cut(s) 3069, 3097, 3468, 5635
Bse8I GATNNNNATC 3 cut(s) 423, 679, 768
BseBI CCWGG 9 cut(s) 244, 1187, 1932, 2817, 3051, 4288, 5331, 5598, 5887
BseCI ATCGAT 1 cut(s) 133
BseDI CCNNGG 9 cut(s) 894, 978, 2641, 2816, 3324, 3525, 3648, 3975, 5597
BseJI GATNNNNATC 3 cut(s) 423, 679, 768
BseLI CCNNNNNNNGG 5 cut(s) 1063, 2867, 3187, 5977, 6118
BseMI GCAATG 4 cut(s) 3069, 3097, 3468, 5635
BseMII CTCAG 6 cut(s) 23, 98, 246, 2987, 3501, 4524
BseRI GAGGAG 5 cut(s) 406, 1166, 3645, 3797, 3800
BseSI GKGCMC 6 cut(s) 1000, 1363, 3353, 4600, 6113, 6146
BseYI CCCAGC 4 cut(s) 734, 1096, 1775, 3902
BsgI GTGCAG 4 cut(s) 820, 3687, 4375, 4401
Bsh1285I CGRYCG 1 cut(s) 382
BshNI GGYRCC 3 cut(s) 110, 1647, 6141
BshVI ATCGAT 1 cut(s) 133
BsiEI CGRYCG 1 cut(s) 382
BsiHKAI GWGCWC 4 cut(s) 1000, 1408, 3808, 4600
BsiHKCI CYCGRG 2 cut(s) 210, 1401
BsiSI CCGG 2 cut(s) 77, 1863
BslFI GGGAC 2 cut(s) 2456, 2667
BslI CCNNNNNNNGG 5 cut(s) 1063, 2867, 3187, 5977, 6118
BsmFI GGGAC 2 cut(s) 2456, 2667
BsmI GAATGC 4 cut(s) 1451, 1915, 2716, 3986
Bso31I GGTCTC 3 cut(s) 2826, 5232, 5607
BsoBI CYCGRG 2 cut(s) 210, 1401
Bsp1286I GDGCHC 8 cut(s) 1000, 1363, 1408, 3353, 3808, 4600, 6113, 6146
Bsp1407I TGTACA 2 cut(s) 3285, 4555
Bsp19I CCATGG 6 cut(s) 894, 978, 2641, 3525, 3648, 3975
BspACI CCGC 4 cut(s) 3473, 4184, 5283, 5348
BspCNI CTCAG 6 cut(s) 22, 97, 245, 2988, 3502, 4525
BspDI ATCGAT 1 cut(s) 133
BspLI GGNNCC 7 cut(s) 112, 1649, 2683, 2727, 3845, 5464, 6143
BspMAI CTGCAG 4 cut(s) 390, 5376, 5682, 6086
BspMI ACCTGC 3 cut(s) 2380, 3031, 4219
BspPI GGATC 6 cut(s) 484, 1168, 2508, 3838, 3851, 4711
BspQI GCTCTTC 3 cut(s) 2385, 2694, 3796
BspT107I GGYRCC 3 cut(s) 110, 1647, 6141
BspTNI GGTCTC 3 cut(s) 2826, 5232, 5607
BsrDI GCAATG 4 cut(s) 3069, 3097, 3468, 5635
BsrGI TGTACA 2 cut(s) 3285, 4555
BssECI CCNNGG 9 cut(s) 894, 978, 2641, 2816, 3324, 3525, 3648, 3975, 5597
BssT1I CCWWGG 7 cut(s) 894, 978, 2641, 3324, 3525, 3648, 3975
Bst2UI CCWGG 9 cut(s) 244, 1187, 1932, 2817, 3051, 4288, 5331, 5598, 5887
BstAUI TGTACA 2 cut(s) 3285, 4555
BstBAI YACGTR 1 cut(s) 2599
BstC8I GCNNGC 6 cut(s) 961, 1667, 1796, 4216, 4454, 4646
BstDSI CCRYGG 6 cut(s) 894, 978, 2641, 3525, 3648, 3975
BstH2I RGCGCY 1 cut(s) 917
BstHHI GCGC 1 cut(s) 916
BstMCI CGRYCG 1 cut(s) 382
BstNI CCWGG 9 cut(s) 244, 1187, 1932, 2817, 3051, 4288, 5331, 5598, 5887
BstNSI RCATGY 9 cut(s) 992, 1004, 2257, 2346, 2957, 3662, 5050, 5192, 5438
BstSFI CTRYAG 7 cut(s) 386, 1901, 2929, 4447, 5372, 5678, 6082
BstSLI GKGCMC 6 cut(s) 1000, 1363, 3353, 4600, 6113, 6146
BstSNI TACGTA 1 cut(s) 2599
BstV2I GAAGAC 7 cut(s) 1124, 1542, 1755, 3165, 4109, 4329, 6285
BstX2I RGATCY 8 cut(s) 424, 769, 1173, 2513, 3843, 4716, 4762, 5950
BstYI RGATCY 8 cut(s) 424, 769, 1173, 2513, 3843, 4716, 4762, 5950
Bsu15I ATCGAT 1 cut(s) 133
BsuI GTATCC 2 cut(s) 1057, 3727
BsuTUI ATCGAT 1 cut(s) 133
BtgI CCRYGG 6 cut(s) 894, 978, 2641, 3525, 3648, 3975
BtsI GCAGTG 4 cut(s) 704, 933, 5057, 6103
BveI ACCTGC 3 cut(s) 2380, 3031, 4219
Cac8I GCNNGC 6 cut(s) 961, 1667, 1796, 4216, 4454, 4646
CaiI CAGNNNCTG 3 cut(s) 302, 2062, 3368
CfoI GCGC 1 cut(s) 916
ClaI ATCGAT 1 cut(s) 133
CsiI ACCWGGT 1 cut(s) 5329
CspCI CAANNNNNGTGG 2 cut(s) 6189, 6224
DraI TTTAAA 2 cut(s) 1788, 1920
DraIII CACNNNGTG 2 cut(s) 787, 4154
EaeI YGGCCR 4 cut(s) 723, 1796, 4512, 4583
Ecl136II GAGCTC 1 cut(s) 3806
Eco105I TACGTA 1 cut(s) 2599
Eco130I CCWWGG 7 cut(s) 894, 978, 2641, 3324, 3525, 3648, 3975
Eco24I GRGCYC 1 cut(s) 3808
Eco31I GGTCTC 3 cut(s) 2826, 5232, 5607
Eco32I GATATC 5 cut(s) 1849, 2223, 3310, 4275, 4333
Eco47I GGWCC 7 cut(s) 691, 2681, 2848, 3047, 3053, 4029, 5837
Eco53kI GAGCTC 1 cut(s) 3806
Eco88I CYCGRG 2 cut(s) 210, 1401
EcoICRI GAGCTC 1 cut(s) 3806
EcoRI GAATTC 2 cut(s) 579, 3897
EcoRII CCWGG 9 cut(s) 242, 1185, 1930, 2815, 3049, 4286, 5329, 5596, 5885
EcoRV GATATC 5 cut(s) 1849, 2223, 3310, 4275, 4333
EcoT14I CCWWGG 7 cut(s) 894, 978, 2641, 3324, 3525, 3648, 3975
EcoT22I ATGCAT 2 cut(s) 2384, 4494
EcoT38I GRGCYC 1 cut(s) 3808
ErhI CCWWGG 7 cut(s) 894, 978, 2641, 3324, 3525, 3648, 3975
FalI AAGNNNNNCTT 4 cut(s) 1493, 1525, 4042, 4074
FaqI GGGAC 2 cut(s) 2456, 2667
FauI CCCGC 1 cut(s) 5290
FauNDI CATATG 2 cut(s) 2655, 3038
FbaI TGATCA 3 cut(s) 418, 2160, 4812
FblI GTMKAC 4 cut(s) 948, 3171, 3430, 3618
FriOI GRGCYC 1 cut(s) 3808
GlaI GCGC 1 cut(s) 915
GsaI CCCAGC 4 cut(s) 738, 1100, 1779, 3906
GsuI CTGGAG 4 cut(s) 165, 1871, 2936, 4805
HaeII RGCGCY 1 cut(s) 917
HapII CCGG 2 cut(s) 77, 1863
HhaI GCGC 1 cut(s) 916
Hin6I GCGC 1 cut(s) 914
HinP1I GCGC 1 cut(s) 914
HincII GTYRAC 3 cut(s) 3406, 4851, 5124
HindII GTYRAC 3 cut(s) 3406, 4851, 5124
HindIII AAGCTT 4 cut(s) 2034, 2096, 4646, 6059
HpaI GTTAAC 1 cut(s) 5124
HpaII CCGG 2 cut(s) 77, 1863
Hpy99I CGWCG 1 cut(s) 4194
HpyCH4IV ACGT 4 cut(s) 903, 2598, 4055, 6158
HpySE526I ACGT 4 cut(s) 903, 2598, 4055, 6158
HspAI GCGC 1 cut(s) 914
KpnI GGTACC 1 cut(s) 1651
Ksp22I TGATCA 3 cut(s) 418, 2160, 4812
KspAI GTTAAC 1 cut(s) 5124
LguI GCTCTTC 3 cut(s) 2385, 2694, 3796
LmnI GCTCC 4 cut(s) 184, 1243, 5087, 5962
MabI ACCWGGT 1 cut(s) 5329
MaeII ACGT 4 cut(s) 903, 2598, 4055, 6158
MfeI CAATTG 6 cut(s) 468, 489, 2410, 3152, 4243, 5783
MflI RGATCY 8 cut(s) 424, 769, 1173, 2513, 3843, 4716, 4762, 5950
MhlI GDGCHC 8 cut(s) 1000, 1363, 1408, 3353, 3808, 4600, 6113, 6146
MlsI TGGCCA 3 cut(s) 1798, 4514, 4585
MluNI TGGCCA 3 cut(s) 1798, 4514, 4585
MlyI GAGTC 2 cut(s) 2530, 5296
MmeI TCCRAC 4 cut(s) 946, 2782, 3616, 5958
Mox20I TGGCCA 3 cut(s) 1798, 4514, 4585
Mph1103I ATGCAT 2 cut(s) 2384, 4494
MroXI GAANNNNTTC 2 cut(s) 1071, 5156
MscI TGGCCA 3 cut(s) 1798, 4514, 4585
MslI CAYNNNNRTG 6 cut(s) 993, 1456, 3336, 3663, 5408, 5640
Msp20I TGGCCA 3 cut(s) 1798, 4514, 4585
MspA1I CMGCKG 6 cut(s) 284, 302, 1096, 1631, 3710, 5918
MspI CCGG 2 cut(s) 77, 1863
MunI CAATTG 6 cut(s) 468, 489, 2410, 3152, 4243, 5783
Mva1269I GAATGC 4 cut(s) 1451, 1915, 2716, 3986
MvaI CCWGG 9 cut(s) 244, 1187, 1932, 2817, 3051, 4288, 5331, 5598, 5887
NciI CCSGG 1 cut(s) 1863
NcoI CCATGG 6 cut(s) 894, 978, 2641, 3525, 3648, 3975
NdeI CATATG 2 cut(s) 2655, 3038
NlaIV GGNNCC 7 cut(s) 112, 1649, 2683, 2727, 3845, 5464, 6143
NmeAIII GCCGAG 1 cut(s) 1627
NmuCI GTSAC 6 cut(s) 16, 931, 2776, 3601, 5350, 5667
NsiI ATGCAT 2 cut(s) 2384, 4494
NspI RCATGY 9 cut(s) 992, 1004, 2257, 2346, 2957, 3662, 5050, 5192, 5438
PaeR7I CTCGAG 2 cut(s) 210, 1401
PciI ACATGT 5 cut(s) 988, 2342, 2953, 3658, 5188
PciSI GCTCTTC 3 cut(s) 2385, 2694, 3796
PctI GAATGC 4 cut(s) 1451, 1915, 2716, 3986
PdmI GAANNNNTTC 2 cut(s) 1071, 5156
PflFI GACNNNGTC 1 cut(s) 929
PfoI TCCNGGA 1 cut(s) 1185
Ple19I CGATCG 1 cut(s) 382
PleI GAGTC 2 cut(s) 2529, 5296
PpsI GAGTC 2 cut(s) 2529, 5296
Ppu21I YACGTR 1 cut(s) 2599
PscI ACATGT 5 cut(s) 988, 2342, 2953, 3658, 5188
PshBI ATTAAT 1 cut(s) 1835
PsiI TTATAA 1 cut(s) 1181
Psp124BI GAGCTC 1 cut(s) 3808
Psp6I CCWGG 9 cut(s) 242, 1185, 1930, 2815, 3049, 4286, 5329, 5596, 5885
PspFI CCCAGC 4 cut(s) 734, 1096, 1775, 3902
PspGI CCWGG 9 cut(s) 242, 1185, 1930, 2815, 3049, 4286, 5329, 5596, 5885
PspN4I GGNNCC 7 cut(s) 112, 1649, 2683, 2727, 3845, 5464, 6143
PspXI VCTCGAGB 1 cut(s) 1401
PstI CTGCAG 4 cut(s) 390, 5376, 5682, 6086
PstNI CAGNNNCTG 3 cut(s) 302, 2062, 3368
PsuI RGATCY 8 cut(s) 424, 769, 1173, 2513, 3843, 4716, 4762, 5950
PsyI GACNNNGTC 1 cut(s) 929
PvuI CGATCG 1 cut(s) 382
PvuII CAGCTG 6 cut(s) 284, 302, 1096, 1631, 3710, 5918
RseI CAYNNNNRTG 6 cut(s) 993, 1456, 3336, 3663, 5408, 5640
SacI GAGCTC 1 cut(s) 3808
SapI GCTCTTC 3 cut(s) 2385, 2694, 3796
SchI GAGTC 2 cut(s) 2530, 5296
SduI GDGCHC 8 cut(s) 1000, 1363, 1408, 3353, 3808, 4600, 6113, 6146
SexAI ACCWGGT 1 cut(s) 5329
SfcI CTRYAG 7 cut(s) 386, 1901, 2929, 4447, 5372, 5678, 6082
Sfr274I CTCGAG 2 cut(s) 210, 1401
SinI GGWCC 7 cut(s) 691, 2681, 2848, 3047, 3053, 4029, 5837
SlaI CTCGAG 2 cut(s) 210, 1401
SmiI ATTTAAAT 1 cut(s) 1920
SmiMI CAYNNNNRTG 6 cut(s) 993, 1456, 3336, 3663, 5408, 5640
SmlI CTYRAG 9 cut(s) 210, 1401, 2099, 2396, 2518, 3138, 3733, 4892, 5756
SmoI CTYRAG 9 cut(s) 210, 1401, 2099, 2396, 2518, 3138, 3733, 4892, 5756
SnaBI TACGTA 1 cut(s) 2599
SpeI ACTAGT 1 cut(s) 1378
SsiI CCGC 4 cut(s) 3473, 4184, 5283, 5348
SspI AATATT 6 cut(s) 1735, 2353, 2875, 4727, 5104, 5986
SstI GAGCTC 1 cut(s) 3808
StyI CCWWGG 7 cut(s) 894, 978, 2641, 3324, 3525, 3648, 3975
SwaI ATTTAAAT 1 cut(s) 1920
TaiI ACGT 4 cut(s) 906, 2601, 4058, 6161
TatI WGTACW 6 cut(s) 808, 835, 3285, 3488, 4555, 6089
TauI GCSGC 1 cut(s) 4187
TseFI GTSAC 6 cut(s) 16, 931, 2776, 3601, 5350, 5667
Tsp45I GTSAC 6 cut(s) 16, 931, 2776, 3601, 5350, 5667
TspGWI ACGGA 5 cut(s) 1297, 3227, 3636, 4491, 4992
Tth111I GACNNNGTC 1 cut(s) 929
VneI GTGCAC 2 cut(s) 996, 4596
VpaK11BI GGWCC 7 cut(s) 691, 2681, 2848, 3047, 3053, 4029, 5837
VspI ATTAAT 1 cut(s) 1835
XapI RAATTY 8 cut(s) 579, 1017, 2205, 3741, 3897, 4638, 4779, 6257
XbaI TCTAGA 3 cut(s) 2044, 2942, 4834
XceI RCATGY 9 cut(s) 992, 1004, 2257, 2346, 2957, 3662, 5050, 5192, 5438
XcmI CCANNNNNNNNNTGG 1 cut(s) 2692
XhoI CTCGAG 2 cut(s) 210, 1401
XmiI GTMKAC 4 cut(s) 948, 3171, 3430, 3618
XmnI GAANNNNTTC 2 cut(s) 1071, 5156
Zsp2I ATGCAT 2 cut(s) 2384, 4494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.