RchiOBHm_Chr5g0079861

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000085
Physical Location & Seq
Reverse (-)
85652142 .. 85657787
5646 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35421

Sequence Viewer

Length: 3378 bp
ATGGCTTTGAGCACCCCAACAGCCTCTGCAACTCTTCATTATGCTGGACCAGCCCCTCGGTGGAGCCATGAAGTGTTTTTAAGTTTCAGGGGTATAGACACTCGCAAAGGTATTACGTCCGATATATACGATAGATTGCAAAACATGAGAGGAATTATAACATTCATGGATGACCAAGAGCTTGAAGTAGGTGATGTTATTTCTCCCAGACTCCTAACCGCAATCAAAGAATCAAGGTTTGCAATAGTTGTTCTGTCGCAACACTATGCTTCTTCTTCCTGGTGTTTGGAGGAACTCACAACGATTTTCCAATGCATGAAAGACAATAACAGAATTCTGCCACTTTTCTATCAAGTGGAACCATCTCACGTACGATATCAGAAAGGGAACTTTGAAGTTGCTTTCACAAAGCATGAGTCGAGGCAAAGCATAGCGAAGGTGAAGCAATGGAGAGATGCTCTACAAAAAGTGGCCAATCTCTCTGGATGGGATTCCAAGAACTATGTTTCTGATAAAGAACTTGTCGAAGACATTGTGAAACTCGTGTGGAGTAGAGTACTTCAAGCACGTATAGAAAATGAGTCAACCACAAGGGATTTTGGATCGTTTAAAGCAACACGAATGGCTATGGGTAAGGTCATGACCGCGCTAAAAAATGATCAAGTCACTGTCATTGGGGTGCACGGTATGGGAGGGGTCGGAAAGACAACCATGGTGAAACATGTTGGTGCACAAGCCCGAAATAACGGGCTTTTTGATAAAGTGATTATGGCTGTTGTATCCCAAAGCCCTGACTTGAAAAAAATTCAAGCCCAACTTGCAGAAATGTTAGCCTTGAAATTGGAGGAGAACACAGAAGAAGGAAGAGCCTATATATTGAAGGAGAGGATAATGAGAGGCAACAGGATTCTTATTATCTTGGATGACATTTGGAGGAAAATAGAATTGTCAAGTATTGGAATCCCCAGCAACAATGAGCTTCAGATGCGCAATTCCAAAATACTACTCACCACAAGGATAATGCATGTATGTGAGGACATGGATACGCAGGCAAAGATCACTCTCAATATTCTGTCAGAAGAAGATTCTTTGAACTTATTCTTGAAGAAAGCAAGGATGTCATTTCATGAATCAACTGAAATCTATAAGGTAGCAAGGAAAGTAGTTCAAAAATGTGGAGGTCTACCAATAGCGTTGATTGCAGTTGCAAGAGCACTTGGAGATAAAGACTTGGAAGAGTGGAAGGAGGCAGCTCAACGACTACAAGTGTCTCAACCTAGCAACCCTGAAGATGAGAGACATGTATTTGAATGTATAAAGCTAAGCTATGATTACTTGAGATCTAATGATGCCAAGTCATGTTTCTTGCTTTGCTGCCTCTTCCCAGAAGACTATGAAATCCCAACTGAAGACTTGTTGAAATATGGGGTCGGGATAGGACTGTTTCAAGATTCCAACACAATGCAAGAAGCCAGAGCTCGAGTAGAATTGGTGGTGAAGTCCCTTAAAGCTTCTAGCTTGCTTTTGGAGCCGAAAGAGAAGGGATGTGTAAGGATGCATGATGTCATCCGCGATGTGGCCATATCAATTTCGTTACTTGAAGATGGTCAACGGTTTTTTGTAAAAGCTGGTTGTGAATTAATGGAGTGGCCAAAGATTAATGCACACGAAGGCTACTCTGTAATCTCACTGATGAAGAACAAAATTAGCAAGCTTCCTGAAGAGTTGGTATGTCCAAAACTCCTTATATTATTACTCCAACATAATGCTACTTTGAATGAGATCCCGAAGACTTTTTTCAAAAGTCTGAATGAATTAAGGGTTTTTGATATAAGTGAATCTAGCATGTGTTCGGTACCCTTATCATTTTGTCCCCTAACCAACCTCCACACTTTGTGTCTGGATGGCTGTAAGTCAATAGGTGACATGTCATTTCTCCAAAGCCTGAATAACCTTGAGATTCTTAGTATGAGAGAATGTCGTCCTCAGGAATTTCCAAAAGAAATTGGACATCTGACCAATTTAAGAATGTTGGACATCACTAACTTGCGGTTGCAGGTCAGTACAATTCCATCTAAAGTGATATCTAGGTTGTCTAAATTAGAAGAACTGTACATGCATTGTGATTTTGGGGACTGGGGGAGTAAAGCAGTTGAGGGAGTTGAAGAAGAAGCTAATGTTGGCTTTGATGAGTTAACTGGCTTATCAGATTTGACCATTTTGAAAGTTCTAATATATGATGCAGAATGCTTGCCCAGAAACGTAAAGTCAAATCCAAATTGGTTTAAATTTGATATATGTATACAGAGAGGCCCAATTCTGAATTTGAAGAAACTGAGAAACCCATCTAAACTTTTTTATAATCCACGTGATCATTATTCAAGAGTCTTGACTCTTTACAGCTCCGTCAATTACTTACCAGGCTGGTTTCTCAATGTGGTGGCAACAAAAACAGAGAAACTTCAGTACATTGATTGCGAGGAGTTGAGTCAGTTGACTAACATTGTTATGGAACATGACCATGGAAAACTGCATGGACTGAAGTATCTCATTATATCCGGGCGCCATGCAAACTTGGAAGAGTTGATGAACTCAACAGGACGGGTTTCTAATAAACCAGTCTTGGAGTACCTGGAAGAGTTACAACTCCGTTCTGTGGATGGCCTGAAACAGTTATGCGTCGGTGAATTACCTTCTCGGTCTCTTTGCAATCTCAAGTTGTTGAAGGTGCTAGGTTGTCAAAATTTGGGGAACACACTTCTGCCATCAAAGTTGTTGCAGAGATTACAAAATCTAGAAAAAATAACTTGTGTAGAAATGAATAGAGTGGAGTACGTGTTTGGATCATCATCATCAGATTGTATTCACCCAGAGCATATGACAAAATTGAGAATGATGAGGTTGTGGTTTCTAGAAAACCTAATATGGATCTGGAAGGGTCCTGCTCCATCTTCCACCTTCCATAATCTTAGAACTTTGGTAGTTGCCGATTGCATGAATCTGAAATGTCTCTTCACATCTGATATAGCTCAAGGTCTTTTGCAATTGGAAGATCTTTGGGTAGAAGACTGCCAGAGCTTGGACAGCCTTATTGAACCAAGTGAGGATACTCTTAAGAACAACAAGATGATCATTGTTTTTCCAAAATTGAAGAACTTGCTTCTGTCTGGTCTTCCAGAGCTTATAGGGTTCTGCCACAGTGGAGATGCTACCATTCATGTTGAGTGCCCTTCATTGGAACACTTGCATCTGAAGGAATGCCCTCAGTTTTCGACCTGTAACTTTCATAGCAGGAACCGATTTCAAGTCAACAATCAACAACATGACGACATTCTATACGATAGATTGTGGGATGTAGATGAGCACATCGTAAAATGA

Protein Analysis

1125

Amino Acids

128.25

Weight (kDa)

6.42

Isoelectric Point (pI)

46.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 23 - 192 3.1e-49 TIR domain
TIR_2 PF13676 25 - 122 3.6e-14 TIR domain
NB-ARC PF00931 210 - 372 5.2e-31 NB-ARC domain
LRR_14 PF23598 589 - 709 1.4e-12 Leucine-rich repeat region
LRR_RPS2 PF23247 878 - 1025 1e-26 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 158, 2361
Acc16I TGCGCA 1 cut(s) 989
Acc36I ACCTGC 1 cut(s) 2047
Acc65I GGTACC 1 cut(s) 1855
AccB1I GGYRCC 2 cut(s) 1855, 2562
AccB7I CCANNNNNTGG 1 cut(s) 3079
AccI GTMKAC 2 cut(s) 1183, 2302
AccII CGCG 2 cut(s) 647, 1572
AciI CCGC 4 cut(s) 219, 645, 1570, 2050
AclWI GGATC 4 cut(s) 610, 1777, 2851, 2936
AcoI YGGCCR 3 cut(s) 471, 1578, 1649
AcsI RAATTY 6 cut(s) 333, 804, 1991, 2288, 2323, 2743
AcuI CTGAAG 7 cut(s) 965, 1308, 1428, 1740, 2447, 2561, 3272
AcvI CACGTG 1 cut(s) 2369
AcyI GRCGYC 1 cut(s) 2563
AdeI CACNNNGTG 1 cut(s) 1896
AfaI GTAC 8 cut(s) 372, 558, 1857, 2065, 2114, 2468, 2630, 2834
AfiI CCNNNNNNNGG 5 cut(s) 60, 1576, 2656, 3079, 3235
AflII CTTAAG 1 cut(s) 3113
AflIII ACRYGT 4 cut(s) 721, 1300, 1926, 2835
AjnI CCWGG 3 cut(s) 278, 2419, 2631
AjuI GAANNNNNNNTTGG 4 cut(s) 1346, 1378, 2163, 2195
Alw21I GWGCWC 6 cut(s) 14, 684, 733, 1216, 1480, 3366
Alw26I GTCTC 4 cut(s) 1275, 1291, 2706, 3014
Alw44I GTGCAC 2 cut(s) 680, 729
AlwI GGATC 4 cut(s) 610, 1777, 2851, 2936
AlwNI CAGNNNCTG 1 cut(s) 26
Ama87I CYCGRG 1 cut(s) 1479
AoxI GGCC 5 cut(s) 471, 1578, 1649, 2311, 2662
ApaLI GTGCAC 2 cut(s) 680, 729
ApeKI GCWGC 2 cut(s) 1250, 1374
ApoI RAATTY 6 cut(s) 333, 804, 1991, 2288, 2323, 2743
ArsI GACNNNNNNTTYG 2 cut(s) 401, 433
AseI ATTAAT 2 cut(s) 1640, 1659
Asp700I GAANNNNTTC 1 cut(s) 1097
Asp718I GGTACC 1 cut(s) 1855
AspLEI GCGC 3 cut(s) 649, 990, 2565
AspS9I GGNCC 3 cut(s) 47, 2312, 2939
AsuC2I CCSGG 1 cut(s) 2560
AsuHPI GGTGA 8 cut(s) 203, 451, 727, 1000, 1507, 1934, 2696, 2858
AvaI CYCGRG 1 cut(s) 1479
AvaII GGWCC 2 cut(s) 47, 2939
AxyI CCTNAGG 1 cut(s) 1986
BaeGI GKGCMC 3 cut(s) 684, 733, 3230
BaeI ACNNNNGTAYC 2 cut(s) 2528, 2561
BalI TGGCCA 3 cut(s) 473, 1580, 1651
BanI GGYRCC 2 cut(s) 1855, 2562
BanII GRGCYC 1 cut(s) 1480
BauI CACGAG 1 cut(s) 542
BbrPI CACGTG 1 cut(s) 2369
BbsI GAAGAC 6 cut(s) 534, 1395, 1416, 1796, 3072, 3164
Bbv12I GWGCWC 6 cut(s) 14, 684, 733, 1216, 1480, 3366
BbvI GCAGC 2 cut(s) 1262, 1361
BccI CCATC 9 cut(s) 370, 480, 1598, 1898, 2080, 2353, 2654, 2773, 2956
BciT130I CCWGG 3 cut(s) 280, 2421, 2633
BciVI GTATCC 3 cut(s) 790, 1036, 3100
BclI TGATCA 3 cut(s) 658, 2371, 3129
BcnI CCSGG 1 cut(s) 2560
BcoDI GTCTC 4 cut(s) 1275, 1291, 2706, 3014
BfaI CTAG 7 cut(s) 1278, 1515, 1842, 2088, 2732, 2795, 2912
BfoI RGCGCY 1 cut(s) 2566
BfrI CTTAAG 1 cut(s) 3113
BfuAI ACCTGC 1 cut(s) 2047
BfuI GTATCC 3 cut(s) 790, 1036, 3100
BglII AGATCT 2 cut(s) 1340, 3052
BisI GCNGC 2 cut(s) 1251, 1375
BlpI GCTNAGC 1 cut(s) 1322
BlsI GCNGC 2 cut(s) 1252, 1376
BmcAI AGTACT 1 cut(s) 558
Bme1390I CCNGG 4 cut(s) 280, 2421, 2560, 2633
Bme18I GGWCC 2 cut(s) 47, 2939
BmeT110I CYCGRG 1 cut(s) 1479
BmgT120I GGNCC 3 cut(s) 47, 2312, 2939
BmiI GGNNCC 7 cut(s) 65, 360, 1530, 1857, 2564, 2940, 3296
BmrFI CCNGG 4 cut(s) 280, 2421, 2560, 2633
BmrI ACTGGG 1 cut(s) 2146
BmsI GCATC 7 cut(s) 445, 975, 1339, 1545, 2230, 3196, 3256
BmuI ACTGGG 1 cut(s) 2146
BpiI GAAGAC 6 cut(s) 534, 1395, 1416, 1796, 3072, 3164
BplI GAGNNNNNCTC 2 cut(s) 442, 474
Bpu1102I GCTNAGC 1 cut(s) 1322
BpuEI CTTGAG 4 cut(s) 1357, 1976, 2699, 3015
BpuMI CCSGG 1 cut(s) 2560
BsaAI YACGTR 4 cut(s) 370, 569, 2369, 2836
BsaBI GATNNNNATC 1 cut(s) 2848
BsaHI GRCGYC 1 cut(s) 2563
BsaI GGTCTC 1 cut(s) 2706
BsaJI CCNNGG 3 cut(s) 56, 711, 2521
BsaXI ACNNNNNCTCC 2 cut(s) 2822, 2852
Bsc4I CCNNNNNNNGG 5 cut(s) 60, 1576, 2656, 3079, 3235
Bse1I ACTGG 3 cut(s) 2141, 2203, 2618
Bse21I CCTNAGG 1 cut(s) 1986
Bse3DI GCAATG 1 cut(s) 452
Bse8I GATNNNNATC 1 cut(s) 2848
BseBI CCWGG 3 cut(s) 280, 2421, 2633
BseDI CCNNGG 3 cut(s) 56, 711, 2521
BseJI GATNNNNATC 1 cut(s) 2848
BseLI CCNNNNNNNGG 5 cut(s) 60, 1576, 2656, 3079, 3235
BseMI GCAATG 1 cut(s) 452
BseMII CTCAG 3 cut(s) 2000, 2327, 3278
BseNI ACTGG 3 cut(s) 2141, 2203, 2618
BseRI GAGGAG 2 cut(s) 860, 2495
BseSI GKGCMC 3 cut(s) 684, 733, 3230
BseXI GCAGC 2 cut(s) 1262, 1361
BseYI CCCAGC 1 cut(s) 965
Bsh1236I CGCG 2 cut(s) 647, 1572
BshFI GGCC 5 cut(s) 473, 1580, 1651, 2313, 2664
BshNI GGYRCC 2 cut(s) 1855, 2562
BsiHKAI GWGCWC 6 cut(s) 14, 684, 733, 1216, 1480, 3366
BsiHKCI CYCGRG 1 cut(s) 1479
BsiSI CCGG 1 cut(s) 2559
BsiWI CGTACG 1 cut(s) 370
BslFI GGGAC 3 cut(s) 1486, 1857, 2147
BslI CCNNNNNNNGG 5 cut(s) 60, 1576, 2656, 3079, 3235
BsmAI GTCTC 4 cut(s) 1275, 1291, 2706, 3014
BsmFI GGGAC 3 cut(s) 1486, 1857, 2147
BsmI GAATGC 2 cut(s) 2252, 3263
BsnI GGCC 5 cut(s) 473, 1580, 1651, 2313, 2664
Bso31I GGTCTC 1 cut(s) 2706
BsoBI CYCGRG 1 cut(s) 1479
Bsp1286I GDGCHC 7 cut(s) 14, 684, 733, 1216, 1480, 3230, 3366
Bsp1407I TGTACA 1 cut(s) 2112
Bsp1720I GCTNAGC 1 cut(s) 1322
Bsp19I CCATGG 2 cut(s) 711, 2521
BspACI CCGC 4 cut(s) 219, 645, 1570, 2050
BspANI GGCC 5 cut(s) 473, 1580, 1651, 2313, 2664
BspCNI CTCAG 3 cut(s) 1999, 2328, 3277
BspFNI CGCG 2 cut(s) 647, 1572
BspHI TCATGA 2 cut(s) 639, 1126
BspLI GGNNCC 7 cut(s) 65, 360, 1530, 1857, 2564, 2940, 3296
BspMI ACCTGC 1 cut(s) 2047
BspPI GGATC 4 cut(s) 610, 1777, 2851, 2936
BspQI GCTCTTC 1 cut(s) 859
BspT107I GGYRCC 2 cut(s) 1855, 2562
BspTI CTTAAG 1 cut(s) 3113
BspTNI GGTCTC 1 cut(s) 2706
BsrDI GCAATG 1 cut(s) 452
BsrGI TGTACA 1 cut(s) 2112
BsrI ACTGG 3 cut(s) 2141, 2203, 2618
BssECI CCNNGG 3 cut(s) 56, 711, 2521
BssNAI GTATAC 1 cut(s) 2303
BssNI GRCGYC 1 cut(s) 2563
BssSI CACGAG 1 cut(s) 542
BssT1I CCWWGG 2 cut(s) 711, 2521
Bst1107I GTATAC 1 cut(s) 2303
Bst2BI CACGAG 1 cut(s) 542
Bst2UI CCWGG 3 cut(s) 280, 2421, 2633
Bst4CI ACNGT 7 cut(s) 670, 686, 1443, 1614, 2112, 2673, 3200
Bst6I CTCTTC 8 cut(s) 39, 859, 1230, 1385, 1716, 2574, 2631, 3017
BstACI GRCGYC 1 cut(s) 2563
BstAFI CTTAAG 1 cut(s) 3113
BstAUI TGTACA 1 cut(s) 2112
BstBAI YACGTR 4 cut(s) 370, 569, 2369, 2836
BstC8I GCNNGC 4 cut(s) 1050, 1520, 1712, 2252
BstDEI CTNAG 6 cut(s) 1322, 1964, 1986, 2336, 2969, 3264
BstDSI CCRYGG 2 cut(s) 711, 2521
BstFNI CGCG 2 cut(s) 647, 1572
BstH2I RGCGCY 1 cut(s) 2566
BstHHI GCGC 3 cut(s) 649, 990, 2565
BstMAI GTCTC 4 cut(s) 1275, 1291, 2706, 3014
BstMWI GCNNNNNNNGC 6 cut(s) 50, 818, 985, 1199, 1528, 3084
BstNI CCWGG 3 cut(s) 280, 2421, 2633
BstNSI RCATGY 6 cut(s) 725, 1028, 1304, 1849, 1930, 2119
BstSCI CCNGG 4 cut(s) 278, 2419, 2558, 2631
BstSLI GKGCMC 3 cut(s) 684, 733, 3230
BstUI CGCG 2 cut(s) 647, 1572
BstV1I GCAGC 2 cut(s) 1262, 1361
BstV2I GAAGAC 6 cut(s) 534, 1395, 1416, 1796, 3072, 3164
BstX2I RGATCY 4 cut(s) 1340, 1782, 2928, 3052
BstYI RGATCY 4 cut(s) 1340, 1782, 2928, 3052
BstZ17I GTATAC 1 cut(s) 2303
Bsu36I CCTNAGG 1 cut(s) 1986
BsuI GTATCC 3 cut(s) 790, 1036, 3100
BsuRI GGCC 5 cut(s) 473, 1580, 1651, 2313, 2664
BtgI CCRYGG 2 cut(s) 711, 2521
BtgZI GCGATG 1 cut(s) 1587
BtsIMutI CAGTG 3 cut(s) 666, 1688, 3205
BveI ACCTGC 1 cut(s) 2047
Cac8I GCNNGC 4 cut(s) 1050, 1520, 1712, 2252
CaiI CAGNNNCTG 1 cut(s) 26
CciI TCATGA 2 cut(s) 639, 1126
CfoI GCGC 3 cut(s) 649, 990, 2565
Cfr13I GGNCC 3 cut(s) 47, 2312, 2939
CseI GACGC 1 cut(s) 2668
Csp6I GTAC 8 cut(s) 371, 557, 1856, 2064, 2113, 2467, 2629, 2833
CviQI GTAC 8 cut(s) 371, 557, 1856, 2064, 2113, 2467, 2629, 2833
DdeI CTNAG 6 cut(s) 1322, 1964, 1986, 2336, 2969, 3264
DinI GGCGCC 1 cut(s) 2564
DraI TTTAAA 2 cut(s) 610, 2287
DraIII CACNNNGTG 1 cut(s) 1896
EaeI YGGCCR 3 cut(s) 471, 1578, 1649
Eam1104I CTCTTC 8 cut(s) 39, 859, 1230, 1385, 1716, 2574, 2631, 3017
EarI CTCTTC 8 cut(s) 39, 859, 1230, 1385, 1716, 2574, 2631, 3017
Ecl136II GAGCTC 1 cut(s) 1478
Eco130I CCWWGG 2 cut(s) 711, 2521
Eco24I GRGCYC 1 cut(s) 1480
Eco31I GGTCTC 1 cut(s) 2706
Eco32I GATATC 2 cut(s) 377, 2085
Eco47I GGWCC 2 cut(s) 47, 2939
Eco53kI GAGCTC 1 cut(s) 1478
Eco57I CTGAAG 7 cut(s) 965, 1308, 1428, 1740, 2447, 2561, 3272
Eco72I CACGTG 1 cut(s) 2369
Eco81I CCTNAGG 1 cut(s) 1986
Eco88I CYCGRG 1 cut(s) 1479
EcoICRI GAGCTC 1 cut(s) 1478
EcoO109I RGGNCCY 1 cut(s) 2939
EcoRI GAATTC 1 cut(s) 333
EcoRII CCWGG 3 cut(s) 278, 2419, 2631
EcoRV GATATC 2 cut(s) 377, 2085
EcoT14I CCWWGG 2 cut(s) 711, 2521
EcoT22I ATGCAT 4 cut(s) 317, 1026, 1560, 2121
EcoT38I GRGCYC 1 cut(s) 1480
EgeI GGCGCC 1 cut(s) 2564
EheI GGCGCC 1 cut(s) 2564
ErhI CCWWGG 2 cut(s) 711, 2521
FalI AAGNNNNNCTT 2 cut(s) 801, 833
FaqI GGGAC 3 cut(s) 1486, 1857, 2147
FauNDI CATATG 1 cut(s) 2877
FbaI TGATCA 3 cut(s) 658, 2371, 3129
FblI GTMKAC 2 cut(s) 1183, 2302
Fnu4HI GCNGC 2 cut(s) 1251, 1375
FriOI GRGCYC 1 cut(s) 1480
Fsp4HI GCNGC 2 cut(s) 1251, 1375
FspBI CTAG 7 cut(s) 1278, 1515, 1842, 2088, 2732, 2795, 2912
FspI TGCGCA 1 cut(s) 989
GlaI GCGC 3 cut(s) 648, 989, 2564
GluI GCNGC 2 cut(s) 1251, 1375
GsaI CCCAGC 1 cut(s) 969
HaeII RGCGCY 1 cut(s) 2566
HaeIII GGCC 5 cut(s) 473, 1580, 1651, 2313, 2664
HapII CCGG 1 cut(s) 2559
HgaI GACGC 1 cut(s) 2668
HhaI GCGC 3 cut(s) 649, 990, 2565
Hin1I GRCGYC 1 cut(s) 2563
Hin6I GCGC 3 cut(s) 647, 988, 2563
HinP1I GCGC 3 cut(s) 647, 988, 2563
HincII GTYRAC 5 cut(s) 585, 1610, 2196, 2496, 3310
HindII GTYRAC 5 cut(s) 585, 1610, 2196, 2496, 3310
HindIII AAGCTT 2 cut(s) 1509, 1712
HpaI GTTAAC 1 cut(s) 2196
HpaII CCGG 1 cut(s) 2559
HphI GGTGA 8 cut(s) 203, 451, 727, 1000, 1507, 1934, 2696, 2858
Hpy166II GTNNAC 9 cut(s) 585, 682, 731, 1184, 1610, 2196, 2303, 2496, 3310
Hpy8I GTNNAC 9 cut(s) 585, 682, 731, 1184, 1610, 2196, 2303, 2496, 3310
Hpy99I CGWCG 1 cut(s) 2684
HpyCH4III ACNGT 7 cut(s) 670, 686, 1443, 1614, 2112, 2673, 3200
HpyCH4IV ACGT 6 cut(s) 116, 369, 568, 2262, 2368, 2835
HpyF10VI GCNNNNNNNGC 6 cut(s) 50, 818, 985, 1199, 1528, 3084
HpyF3I CTNAG 6 cut(s) 1322, 1964, 1986, 2336, 2969, 3264
HpySE526I ACGT 6 cut(s) 116, 369, 568, 2262, 2368, 2835
Hsp92I GRCGYC 1 cut(s) 2563
HspAI GCGC 3 cut(s) 647, 988, 2563
KasI GGCGCC 1 cut(s) 2562
KpnI GGTACC 1 cut(s) 1859
Ksp22I TGATCA 3 cut(s) 658, 2371, 3129
KspAI GTTAAC 1 cut(s) 2196
LguI GCTCTTC 1 cut(s) 859
LmnI GCTCC 4 cut(s) 63, 1528, 2408, 2950
Lsp1109I GCAGC 2 cut(s) 1262, 1361
LweI GCATC 7 cut(s) 445, 975, 1339, 1545, 2230, 3196, 3256
MaeI CTAG 7 cut(s) 1278, 1515, 1842, 2088, 2732, 2795, 2912
MaeII ACGT 6 cut(s) 116, 369, 568, 2262, 2368, 2835
MaeIII GTNAC 5 cut(s) 664, 1593, 1922, 2640, 3278
MfeI CAATTG 1 cut(s) 3044
MflI RGATCY 4 cut(s) 1340, 1782, 2928, 3052
MhlI GDGCHC 7 cut(s) 14, 684, 733, 1216, 1480, 3230, 3366
MlsI TGGCCA 3 cut(s) 473, 1580, 1651
MluNI TGGCCA 3 cut(s) 473, 1580, 1651
Mly113I GGCGCC 1 cut(s) 2563
MlyI GAGTC 6 cut(s) 204, 425, 590, 2386, 2394, 2497
MmeI TCCRAC 4 cut(s) 679, 1479, 1783, 2013
Mox20I TGGCCA 3 cut(s) 473, 1580, 1651
Mph1103I ATGCAT 4 cut(s) 317, 1026, 1560, 2121
MroXI GAANNNNTTC 1 cut(s) 1097
MscI TGGCCA 3 cut(s) 473, 1580, 1651
MslI CAYNNNNRTG 5 cut(s) 677, 726, 1029, 2507, 2520
Msp20I TGGCCA 3 cut(s) 473, 1580, 1651
MspCI CTTAAG 1 cut(s) 3113
MspI CCGG 1 cut(s) 2559
MspR9I CCNGG 4 cut(s) 280, 2421, 2560, 2633
MunI CAATTG 1 cut(s) 3044
Mva1269I GAATGC 2 cut(s) 2252, 3263
MvaI CCWGG 3 cut(s) 280, 2421, 2633
MvnI CGCG 2 cut(s) 647, 1572
MwoI GCNNNNNNNGC 6 cut(s) 50, 818, 985, 1199, 1528, 3084
NarI GGCGCC 1 cut(s) 2563
NciI CCSGG 1 cut(s) 2560
NcoI CCATGG 2 cut(s) 711, 2521
NdeI CATATG 1 cut(s) 2877
NlaIV GGNNCC 7 cut(s) 65, 360, 1530, 1857, 2564, 2940, 3296
NmuCI GTSAC 2 cut(s) 664, 1922
NsbI TGCGCA 1 cut(s) 989
NsiI ATGCAT 4 cut(s) 317, 1026, 1560, 2121
NspI RCATGY 6 cut(s) 725, 1028, 1304, 1849, 1930, 2119
PaeR7I CTCGAG 1 cut(s) 1479
PagI TCATGA 2 cut(s) 639, 1126
PciI ACATGT 3 cut(s) 721, 1300, 1926
PciSI GCTCTTC 1 cut(s) 859
PctI GAATGC 2 cut(s) 2252, 3263
PdmI GAANNNNTTC 1 cut(s) 1097
Pfl23II CGTACG 1 cut(s) 370
PflMI CCANNNNNTGG 1 cut(s) 3079
PkrI GCNGC 2 cut(s) 1252, 1376
PleI GAGTC 6 cut(s) 204, 424, 589, 2386, 2393, 2496
PluTI GGCGCC 1 cut(s) 2566
PmaCI CACGTG 1 cut(s) 2369
PmlI CACGTG 1 cut(s) 2369
PpsI GAGTC 6 cut(s) 204, 424, 589, 2386, 2393, 2496
Ppu21I YACGTR 4 cut(s) 370, 569, 2369, 2836
PpuMI RGGWCCY 1 cut(s) 2939
PscI ACATGT 3 cut(s) 721, 1300, 1926
PshBI ATTAAT 2 cut(s) 1640, 1659
PsiI TTATAA 2 cut(s) 158, 2361
Psp124BI GAGCTC 1 cut(s) 1480
Psp5II RGGWCCY 1 cut(s) 2939
Psp6I CCWGG 3 cut(s) 278, 2419, 2631
PspCI CACGTG 1 cut(s) 2369
PspFI CCCAGC 1 cut(s) 965
PspGI CCWGG 3 cut(s) 278, 2419, 2631
PspLI CGTACG 1 cut(s) 370
PspN4I GGNNCC 7 cut(s) 65, 360, 1530, 1857, 2564, 2940, 3296
PspPI GGNCC 3 cut(s) 47, 2312, 2939
PspPPI RGGWCCY 1 cut(s) 2939
PspXI VCTCGAGB 1 cut(s) 1479
PstNI CAGNNNCTG 1 cut(s) 26
PsuI RGATCY 4 cut(s) 1340, 1782, 2928, 3052
RsaI GTAC 8 cut(s) 372, 558, 1857, 2065, 2114, 2468, 2630, 2834
RsaNI GTAC 8 cut(s) 371, 557, 1856, 2064, 2113, 2467, 2629, 2833
RseI CAYNNNNRTG 5 cut(s) 677, 726, 1029, 2507, 2520
SacI GAGCTC 1 cut(s) 1480
SapI GCTCTTC 1 cut(s) 859
SatI GCNGC 2 cut(s) 1251, 1375
Sau96I GGNCC 3 cut(s) 47, 2312, 2939
ScaI AGTACT 1 cut(s) 558
SchI GAGTC 6 cut(s) 204, 425, 590, 2386, 2394, 2497
ScrFI CCNGG 4 cut(s) 280, 2421, 2560, 2633
SduI GDGCHC 7 cut(s) 14, 684, 733, 1216, 1480, 3230, 3366
SfaNI GCATC 7 cut(s) 445, 975, 1339, 1545, 2230, 3196, 3256
SfoI GGCGCC 1 cut(s) 2564
Sfr274I CTCGAG 1 cut(s) 1479
SinI GGWCC 2 cut(s) 47, 2939
SlaI CTCGAG 1 cut(s) 1479
SmiMI CAYNNNNRTG 5 cut(s) 677, 726, 1029, 2507, 2520
SmlI CTYRAG 6 cut(s) 1336, 1479, 1955, 2714, 3030, 3113
SmoI CTYRAG 6 cut(s) 1336, 1479, 1955, 2714, 3030, 3113
SsiI CCGC 4 cut(s) 219, 645, 1570, 2050
SspDI GGCGCC 1 cut(s) 2562
SspI AATATT 1 cut(s) 1069
SspMI CTAG 7 cut(s) 1278, 1515, 1842, 2088, 2732, 2795, 2912
SstI GAGCTC 1 cut(s) 1480
StyD4I CCNGG 4 cut(s) 278, 2419, 2558, 2631
StyI CCWWGG 2 cut(s) 711, 2521
TaaI ACNGT 7 cut(s) 670, 686, 1443, 1614, 2112, 2673, 3200
TaiI ACGT 6 cut(s) 119, 372, 571, 2265, 2371, 2838
TaqI TCGA 4 cut(s) 419, 525, 1480, 3272
TaqII GACCGA 1 cut(s) 2688
TatI WGTACW 4 cut(s) 556, 2063, 2112, 2466
TscAI CASTG 3 cut(s) 673, 1695, 3205
TseFI GTSAC 2 cut(s) 664, 1922
TseI GCWGC 2 cut(s) 1250, 1374
Tsp45I GTSAC 2 cut(s) 664, 1922
TspGWI ACGGA 2 cut(s) 2395, 2639
TspRI CASTG 3 cut(s) 673, 1695, 3205
Van91I CCANNNNNTGG 1 cut(s) 3079
Vha464I CTTAAG 1 cut(s) 3113
VneI GTGCAC 2 cut(s) 680, 729
VpaK11BI GGWCC 2 cut(s) 47, 2939
VspI ATTAAT 2 cut(s) 1640, 1659
XapI RAATTY 6 cut(s) 333, 804, 1991, 2288, 2323, 2743
XbaI TCTAGA 2 cut(s) 2794, 2911
XceI RCATGY 6 cut(s) 725, 1028, 1304, 1849, 1930, 2119
XcmI CCANNNNNNNNNTGG 1 cut(s) 57
XhoI CTCGAG 1 cut(s) 1479
XmiI GTMKAC 2 cut(s) 1183, 2302
XmnI GAANNNNTTC 1 cut(s) 1097
XspI CTAG 7 cut(s) 1278, 1515, 1842, 2088, 2732, 2795, 2912
ZrmI AGTACT 1 cut(s) 558
Zsp2I ATGCAT 4 cut(s) 317, 1026, 1560, 2121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.