RchiOBHm_Chr5g0061331

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000085
Physical Location & Seq
Reverse (-)
66731208 .. 66733671
2464 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33771

Sequence Viewer

Length: 2043 bp
ATGGATGAGGTCATGAAGGTATTAAAAGATGATCAAGTCACTGTCCTTGGGGTGCATGGAGTGGCTGGCGTTGGAAAGACGACGATGGTCAAACATGTTGGTGCACAATCTAAGAAAGATGGGCTTTTTGATCATGTGATCATGGTTGTCGTATCCAAAAACCCTGACTTGGAAGAAATTCAAGACACATTGGCAGAATCGCTGGGCTTGAAGGAGGGGATCATCAGAGGAAATAGGATGCTTATAATCTTGGATGACATTTGGGGGAGTCTTGAACTCTCGCGCATTGGAATTCCCAGTCACAATGAGCTTCAAAGGTGCAATTCTAAAGTTGTACTCACCACAAGGAAAAGCGACGTCTGCCATTTTATGGGGTGCCATGCTAATGCTCAGATCCTTCTCAATGTTCTATCAGAGGGAGATTCTTGGAAGTTATTCATGGAGACGTCGGGGAAGTCCTTTCATGAATCTACCATTTCCTATGATGAAGCGAGAAAGGTAGCTCGAGAATGCGGTGGTCTCCCAAAAGCAATAAAAGCAGCTGCAAGGTCACTTCGAGATAAATACTTGGACGAATGGAATGAAGCACTTGCAAGGGAATTTGGAGATGACTGCTTGGACGAATGGAATGAAACAGCTCATCCTGTCAACTTCAAAGCAAAGAATGACGCATTTGATTCATTGGCAACTCATGATGCCACGTCATGCTTCTTGCTATGCTGCCTATTCCCAGAAAATTATGATATCCCAATTGAAAAGTTGCTCAAGTATGGGATTGGAATAGGTTTGTTTCAATATGATACACATACAATGCAAGAAGCCAGAGATAGAGCACAATCTGTGGTCAACTACCTCAAAGCTTCTAGATTGCTTTTGGACGGTACAAAGAAGGGATGCACAAGGATGACTGATGACATTCGAAATCTGGCCATGCCCGTTATGCGGTCCCAAGAAGGTGATGGGTTTTTAGTAAAAGCTGGTGAACTAGAGAAGTGGCCAGAGGCAGGATTGTGGCAAGGAATTACTTCCCGCCTGCGCAAAATCTGGGATTGGCCAATAGATGCTCATGAAGGCTACTCAATAATCTCACTCATGAAAAACAAGCTTCATAAGCTTCCCAAATCTTTGGCATGTTCAGAGCTCCAAATTTTATTGTTACAAAGTAATGCTGGTATACGTGACATCCCAGAAAACTTTCTTCAAAGTGCTAATGCGCTAAGGGTCTTTGATCTTAGCTTCACTAGCATTTCATCACTATCTTCATCATTCAGTTTCCTAACCAACCTTCAAGTTTTGAATTTGGATTTATGCCATGATTTAAGTAACATATCCGTTCTAGGAAAACTTAAGAAACTTGAGATTCTTAGTATGAGAGAACTTCCTCTTAAGAAATTTCCAAAACAAATAGGAGATTTGACCAGTCTAAGGATGCTGGACGTCACTGGTAGAGATCTTGAAACAATCCCATCTAAAGTGATCTCGAGGTTGTATAGATTGGAAGAACTGCACATGAAATTTCAGTTTGGGAAGTGGGGAAGTAAAATTGAGGATGCAGTTGAAGAAACCAATGCTGACTTTGATGAGTTGACTGGCTTGTTGGATTTAAACATTTTGGGAGTTTACATATCTGATGCTGCATGCTTGCCTAGAAGTGTTGAGTACAAGCCGAATTGGGTAGAATTTGATATATGCATCAGCAGAGATGCCCCTTGCGTGTCGCTCCCTCAACATGATGGTTTTTCAAGAACCTTGACTCTTGACACAACAATCAATAGCTTACCGGATTGGTTTATCAGCGTTGTGACAGAGAAAGTGGAGAAGCTAGAGTACACGGATTGTGAAGACTTGAATGACATCGTTGGGCAATATGACCATGGCAGATTACTTGCACTGAAATATCTCTCTGTGAACGGGCACTGTAAGAACTTGAAAGAGATAATCAACACAATGACACGGGTTTCAAATAAACCAGTATTTGAGAACTTAGAACAGTTGCATCTGTACCGCTTATATTCCCTCAAGGAGTTATGTGTTGCTTGGTGA

Protein Analysis

680

Amino Acids

76.76

Weight (kDa)

5.6

Isoelectric Point (pI)

33.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 1 - 150 2e-25 NB-ARC domain
LRR_8 PF13855 380 - 435 6.5e-06 Leucine rich repeat
LRR_14 PF23598 414 - 505 2.6e-11 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 245
AatII GACGTC 3 cut(s) 360, 449, 1440
Acc16I TGCGCA 1 cut(s) 1037
AccB1I GGYRCC 1 cut(s) 375
AccB7I CCANNNNNTGG 1 cut(s) 370
AccI GTMKAC 1 cut(s) 1174
AccII CGCG 1 cut(s) 283
AciI CCGC 4 cut(s) 513, 943, 1030, 2005
AclWI GGATC 2 cut(s) 227, 388
AcoI YGGCCR 3 cut(s) 927, 995, 1052
AcsI RAATTY 8 cut(s) 177, 291, 599, 1146, 1297, 1391, 1514, 1679
AcyI GRCGYC 3 cut(s) 357, 446, 1437
AfaI GTAC 5 cut(s) 336, 883, 1661, 1829, 2003
AfiI CCNNNNNNNGG 5 cut(s) 169, 370, 942, 1004, 1425
AflII CTTAAG 2 cut(s) 1346, 1385
AflIII ACRYGT 1 cut(s) 94
AjiI CACGTC 1 cut(s) 702
Alw21I GWGCWC 3 cut(s) 106, 835, 1143
Alw26I GTCTC 2 cut(s) 437, 524
Alw44I GTGCAC 1 cut(s) 102
AlwI GGATC 2 cut(s) 227, 388
Ama87I CYCGRG 2 cut(s) 504, 1480
AoxI GGCC 3 cut(s) 927, 995, 1052
ApaLI GTGCAC 1 cut(s) 102
ApeKI GCWGC 4 cut(s) 539, 542, 720, 1634
ApoI RAATTY 8 cut(s) 177, 291, 599, 1146, 1297, 1391, 1514, 1679
Asp700I GAANNNNTTC 4 cut(s) 177, 434, 1024, 1194
AspLEI GCGC 3 cut(s) 285, 1038, 1216
AspS9I GGNCC 1 cut(s) 945
AsuHPI GGTGA 3 cut(s) 331, 968, 992
AsuII TTCGAA 1 cut(s) 919
AvaI CYCGRG 2 cut(s) 504, 1480
AvaII GGWCC 1 cut(s) 945
BaeGI GKGCMC 2 cut(s) 106, 1917
BalI TGGCCA 3 cut(s) 929, 997, 1054
BanI GGYRCC 1 cut(s) 375
BanII GRGCYC 1 cut(s) 1143
BarI GAAGNNNNNNTAC 2 cut(s) 1811, 1843
BbsI GAAGAC 1 cut(s) 1848
Bbv12I GWGCWC 3 cut(s) 106, 835, 1143
BbvI GCAGC 4 cut(s) 529, 551, 707, 1621
BccI CCATC 5 cut(s) 79, 113, 953, 1474, 1727
BciVI GTATCC 1 cut(s) 163
BclI TGATCA 3 cut(s) 31, 130, 138
BcoDI GTCTC 2 cut(s) 437, 524
BfaI CTAG 6 cut(s) 864, 986, 1242, 1337, 1647, 1823
BfrI CTTAAG 2 cut(s) 1346, 1385
BfuI GTATCC 1 cut(s) 163
BglII AGATCT 1 cut(s) 1450
BisI GCNGC 4 cut(s) 540, 543, 721, 1635
BlsI GCNGC 4 cut(s) 541, 544, 722, 1636
Bme18I GGWCC 1 cut(s) 945
BmeT110I CYCGRG 2 cut(s) 504, 1480
BmgBI CACGTC 1 cut(s) 702
BmgT120I GGNCC 1 cut(s) 945
BmiI GGNNCC 2 cut(s) 377, 947
BmrI ACTGGG 1 cut(s) 291
BmuI ACTGGG 1 cut(s) 291
BoxI GACNNNNGTC 1 cut(s) 86
BpiI GAAGAC 1 cut(s) 1848
Bpu10I CCTNAGC 1 cut(s) 1217
Bpu14I TTCGAA 1 cut(s) 919
BpuEI CTTGAG 3 cut(s) 749, 1376, 2003
BsaAI YACGTR 1 cut(s) 1178
BsaHI GRCGYC 3 cut(s) 357, 446, 1437
BsaI GGTCTC 1 cut(s) 524
BsaJI CCNNGG 2 cut(s) 46, 1873
BsaWI WCCGGW 1 cut(s) 1780
Bsc4I CCNNNNNNNGG 5 cut(s) 169, 370, 942, 1004, 1425
Bse1I ACTGG 5 cut(s) 297, 1419, 1447, 1594, 1970
BseDI CCNNGG 2 cut(s) 46, 1873
BseGI GGATG 9 cut(s) 10, 243, 259, 640, 899, 909, 1182, 1434, 1555
BseLI CCNNNNNNNGG 5 cut(s) 169, 370, 942, 1004, 1425
BseMII CTCAG 1 cut(s) 404
BseNI ACTGG 5 cut(s) 297, 1419, 1447, 1594, 1970
BseSI GKGCMC 2 cut(s) 106, 1917
BseXI GCAGC 4 cut(s) 529, 551, 707, 1621
BseYI CCCAGC 1 cut(s) 202
BsgI GTGCAG 1 cut(s) 1490
Bsh1236I CGCG 1 cut(s) 283
BshFI GGCC 3 cut(s) 929, 997, 1054
BshNI GGYRCC 1 cut(s) 375
BsiHKAI GWGCWC 3 cut(s) 106, 835, 1143
BsiHKCI CYCGRG 2 cut(s) 504, 1480
BsiSI CCGG 1 cut(s) 1781
BslFI GGGAC 1 cut(s) 931
BslI CCNNNNNNNGG 5 cut(s) 169, 370, 942, 1004, 1425
BsmAI GTCTC 2 cut(s) 437, 524
BsmBI CGTCTC 1 cut(s) 437
BsmFI GGGAC 1 cut(s) 931
BsmI GAATGC 1 cut(s) 515
BsnI GGCC 3 cut(s) 929, 997, 1054
Bso31I GGTCTC 1 cut(s) 524
BsoBI CYCGRG 2 cut(s) 504, 1480
Bsp119I TTCGAA 1 cut(s) 919
Bsp1286I GDGCHC 4 cut(s) 106, 835, 1143, 1917
Bsp143I GATC 8 cut(s) 31, 130, 138, 219, 393, 1228, 1450, 1476
Bsp19I CCATGG 1 cut(s) 1873
BspACI CCGC 4 cut(s) 513, 943, 1030, 2005
BspANI GGCC 3 cut(s) 929, 997, 1054
BspCNI CTCAG 1 cut(s) 403
BspFNI CGCG 1 cut(s) 283
BspHI TCATGA 5 cut(s) 12, 463, 691, 1066, 1092
BspLI GGNNCC 2 cut(s) 377, 947
BspPI GGATC 2 cut(s) 227, 388
BspT104I TTCGAA 1 cut(s) 919
BspT107I GGYRCC 1 cut(s) 375
BspTI CTTAAG 2 cut(s) 1346, 1385
BspTNI GGTCTC 1 cut(s) 524
BsrI ACTGG 5 cut(s) 297, 1419, 1447, 1594, 1970
BssECI CCNNGG 2 cut(s) 46, 1873
BssMI GATC 8 cut(s) 31, 130, 138, 219, 393, 1228, 1450, 1476
BssNAI GTATAC 1 cut(s) 1175
BssNI GRCGYC 3 cut(s) 357, 446, 1437
BssT1I CCWWGG 2 cut(s) 46, 1873
Bst1107I GTATAC 1 cut(s) 1175
Bst4CI ACNGT 4 cut(s) 43, 881, 1919, 1992
BstACI GRCGYC 3 cut(s) 357, 446, 1437
BstAFI CTTAAG 2 cut(s) 1346, 1385
BstBAI YACGTR 1 cut(s) 1178
BstBI TTCGAA 1 cut(s) 919
BstC8I GCNNGC 4 cut(s) 67, 1034, 1639, 1643
BstDEI CTNAG 7 cut(s) 111, 390, 1217, 1232, 1364, 1424, 1984
BstDSI CCRYGG 1 cut(s) 1873
BstF5I GGATG 9 cut(s) 10, 243, 259, 640, 899, 909, 1182, 1434, 1555
BstFNI CGCG 1 cut(s) 283
BstHHI GCGC 3 cut(s) 285, 1038, 1216
BstKTI GATC 8 cut(s) 34, 133, 141, 222, 396, 1231, 1453, 1479
BstMAI GTCTC 2 cut(s) 437, 524
BstMBI GATC 8 cut(s) 31, 130, 138, 219, 393, 1228, 1450, 1476
BstMWI GCNNNNNNNGC 5 cut(s) 360, 536, 940, 1111, 1242
BstNSI RCATGY 3 cut(s) 98, 1134, 1641
BstPAI GACNNNNGTC 1 cut(s) 86
BstSLI GKGCMC 2 cut(s) 106, 1917
BstUI CGCG 1 cut(s) 283
BstV1I GCAGC 4 cut(s) 529, 551, 707, 1621
BstV2I GAAGAC 1 cut(s) 1848
BstX2I RGATCY 2 cut(s) 393, 1450
BstXI CCANNNNNNTGG 1 cut(s) 1126
BstYI RGATCY 2 cut(s) 393, 1450
BstZ17I GTATAC 1 cut(s) 1175
BsuI GTATCC 1 cut(s) 163
BsuRI GGCC 3 cut(s) 929, 997, 1054
BtgI CCRYGG 1 cut(s) 1873
BtrI CACGTC 1 cut(s) 702
BtsCI GGATG 9 cut(s) 10, 243, 259, 640, 899, 909, 1182, 1434, 1555
BtsIMutI CAGTG 4 cut(s) 39, 1440, 1889, 1915
Cac8I GCNNGC 4 cut(s) 67, 1034, 1639, 1643
CciI TCATGA 5 cut(s) 12, 463, 691, 1066, 1092
CfoI GCGC 3 cut(s) 285, 1038, 1216
Cfr13I GGNCC 1 cut(s) 945
CseI GACGC 1 cut(s) 677
Csp6I GTAC 5 cut(s) 335, 882, 1660, 1828, 2002
CviQI GTAC 5 cut(s) 335, 882, 1660, 1828, 2002
DdeI CTNAG 7 cut(s) 111, 390, 1217, 1232, 1364, 1424, 1984
DpnI GATC 8 cut(s) 33, 132, 140, 221, 395, 1230, 1452, 1478
DpnII GATC 8 cut(s) 31, 130, 138, 219, 393, 1228, 1450, 1476
DraI TTTAAA 1 cut(s) 1605
EaeI YGGCCR 3 cut(s) 927, 995, 1052
Ecl136II GAGCTC 1 cut(s) 1141
Eco130I CCWWGG 2 cut(s) 46, 1873
Eco24I GRGCYC 1 cut(s) 1143
Eco31I GGTCTC 1 cut(s) 524
Eco32I GATATC 1 cut(s) 745
Eco47I GGWCC 1 cut(s) 945
Eco53kI GAGCTC 1 cut(s) 1141
Eco88I CYCGRG 2 cut(s) 504, 1480
EcoICRI GAGCTC 1 cut(s) 1141
EcoRI GAATTC 1 cut(s) 291
EcoRV GATATC 1 cut(s) 745
EcoT14I CCWWGG 2 cut(s) 46, 1873
EcoT22I ATGCAT 1 cut(s) 1694
EcoT38I GRGCYC 1 cut(s) 1143
ErhI CCWWGG 2 cut(s) 46, 1873
Esp3I CGTCTC 1 cut(s) 437
FalI AAGNNNNNCTT 2 cut(s) 108, 140
FaqI GGGAC 1 cut(s) 931
FauI CCCGC 1 cut(s) 1037
FbaI TGATCA 3 cut(s) 31, 130, 138
FblI GTMKAC 1 cut(s) 1174
Fnu4HI GCNGC 4 cut(s) 540, 543, 721, 1635
FokI GGATG 9 cut(s) 17, 250, 266, 627, 906, 916, 1169, 1441, 1562
FriOI GRGCYC 1 cut(s) 1143
Fsp4HI GCNGC 4 cut(s) 540, 543, 721, 1635
FspBI CTAG 6 cut(s) 864, 986, 1242, 1337, 1647, 1823
FspI TGCGCA 1 cut(s) 1037
GlaI GCGC 3 cut(s) 284, 1037, 1215
GluI GCNGC 4 cut(s) 540, 543, 721, 1635
GsaI CCCAGC 1 cut(s) 206
HaeIII GGCC 3 cut(s) 929, 997, 1054
HapII CCGG 1 cut(s) 1781
HgaI GACGC 1 cut(s) 677
HhaI GCGC 3 cut(s) 285, 1038, 1216
Hin1I GRCGYC 3 cut(s) 357, 446, 1437
Hin6I GCGC 3 cut(s) 283, 1036, 1214
HinP1I GCGC 3 cut(s) 283, 1036, 1214
HincII GTYRAC 3 cut(s) 649, 847, 1587
HindII GTYRAC 3 cut(s) 649, 847, 1587
HindIII AAGCTT 3 cut(s) 858, 1103, 1112
HinfI GANTC 7 cut(s) 197, 268, 422, 467, 677, 1360, 1753
HpaII CCGG 1 cut(s) 1781
HphI GGTGA 3 cut(s) 331, 968, 992
Hpy166II GTNNAC 9 cut(s) 104, 649, 847, 983, 1175, 1587, 1621, 1830, 1909
Hpy188I TCNGA 5 cut(s) 227, 393, 415, 1138, 1630
Hpy8I GTNNAC 9 cut(s) 104, 649, 847, 983, 1175, 1587, 1621, 1830, 1909
Hpy99I CGWCG 3 cut(s) 85, 359, 451
HpyAV CCTTC 7 cut(s) 10, 205, 407, 883, 947, 1064, 1295
HpyCH4III ACNGT 4 cut(s) 43, 881, 1919, 1992
HpyCH4IV ACGT 5 cut(s) 357, 446, 701, 1177, 1437
HpyF10VI GCNNNNNNNGC 5 cut(s) 360, 536, 940, 1111, 1242
HpyF3I CTNAG 7 cut(s) 111, 390, 1217, 1232, 1364, 1424, 1984
HpySE526I ACGT 5 cut(s) 357, 446, 701, 1177, 1437
Hsp92I GRCGYC 3 cut(s) 357, 446, 1437
HspAI GCGC 3 cut(s) 283, 1036, 1214
Ksp22I TGATCA 3 cut(s) 31, 130, 138
Kzo9I GATC 8 cut(s) 31, 130, 138, 219, 393, 1228, 1450, 1476
LmnI GCTCC 2 cut(s) 1146, 1725
Lsp1109I GCAGC 4 cut(s) 529, 551, 707, 1621
MaeI CTAG 6 cut(s) 864, 986, 1242, 1337, 1647, 1823
MaeII ACGT 5 cut(s) 357, 446, 701, 1177, 1437
MaeIII GTNAC 8 cut(s) 37, 299, 549, 1155, 1178, 1322, 1438, 1801
MalI GATC 8 cut(s) 33, 132, 140, 221, 395, 1230, 1452, 1478
MboI GATC 8 cut(s) 31, 130, 138, 219, 393, 1228, 1450, 1476
MboII GAAGA 6 cut(s) 185, 1190, 1251, 1511, 1571, 1853
MfeI CAATTG 1 cut(s) 750
MflI RGATCY 2 cut(s) 393, 1450
MhlI GDGCHC 4 cut(s) 106, 835, 1143, 1917
MlsI TGGCCA 3 cut(s) 929, 997, 1054
MluNI TGGCCA 3 cut(s) 929, 997, 1054
MlyI GAGTC 2 cut(s) 277, 1747
MmeI TCCRAC 2 cut(s) 52, 1578
Mox20I TGGCCA 3 cut(s) 929, 997, 1054
Mph1103I ATGCAT 1 cut(s) 1694
MroXI GAANNNNTTC 4 cut(s) 177, 434, 1024, 1194
MscI TGGCCA 3 cut(s) 929, 997, 1054
MseI TTAA 5 cut(s) 23, 1319, 1347, 1386, 1604
MslI CAYNNNNRTG 3 cut(s) 99, 384, 902
Msp20I TGGCCA 3 cut(s) 929, 997, 1054
MspA1I CMGCKG 1 cut(s) 542
MspCI CTTAAG 2 cut(s) 1346, 1385
MspI CCGG 1 cut(s) 1781
MunI CAATTG 1 cut(s) 750
Mva1269I GAATGC 1 cut(s) 515
MvnI CGCG 1 cut(s) 283
MwoI GCNNNNNNNGC 5 cut(s) 360, 536, 940, 1111, 1242
NcoI CCATGG 1 cut(s) 1873
NdeII GATC 8 cut(s) 31, 130, 138, 219, 393, 1228, 1450, 1476
NlaIV GGNNCC 2 cut(s) 377, 947
NmuCI GTSAC 6 cut(s) 37, 299, 549, 1178, 1438, 1801
NsbI TGCGCA 1 cut(s) 1037
NsiI ATGCAT 1 cut(s) 1694
NspI RCATGY 3 cut(s) 98, 1134, 1641
NspV TTCGAA 1 cut(s) 919
PaeI GCATGC 1 cut(s) 1641
PaeR7I CTCGAG 2 cut(s) 504, 1480
PagI TCATGA 5 cut(s) 12, 463, 691, 1066, 1092
PciI ACATGT 1 cut(s) 94
PctI GAATGC 1 cut(s) 515
PdmI GAANNNNTTC 4 cut(s) 177, 434, 1024, 1194
PfeI GAWTC 5 cut(s) 197, 422, 467, 677, 1360
PflMI CCANNNNNTGG 1 cut(s) 370
PkrI GCNGC 4 cut(s) 541, 544, 722, 1636
PleI GAGTC 2 cut(s) 276, 1747
PpsI GAGTC 2 cut(s) 276, 1747
Ppu21I YACGTR 1 cut(s) 1178
PscI ACATGT 1 cut(s) 94
PshAI GACNNNNGTC 1 cut(s) 86
PsiI TTATAA 1 cut(s) 245
Psp124BI GAGCTC 1 cut(s) 1143
PspFI CCCAGC 1 cut(s) 202
PspN4I GGNNCC 2 cut(s) 377, 947
PspPI GGNCC 1 cut(s) 945
PsuI RGATCY 2 cut(s) 393, 1450
PvuII CAGCTG 1 cut(s) 542
RsaI GTAC 5 cut(s) 336, 883, 1661, 1829, 2003
RsaNI GTAC 5 cut(s) 335, 882, 1660, 1828, 2002
RseI CAYNNNNRTG 3 cut(s) 99, 384, 902
SacI GAGCTC 1 cut(s) 1143
SaqAI TTAA 5 cut(s) 23, 1319, 1347, 1386, 1604
SatI GCNGC 4 cut(s) 540, 543, 721, 1635
Sau3AI GATC 8 cut(s) 31, 130, 138, 219, 393, 1228, 1450, 1476
Sau96I GGNCC 1 cut(s) 945
SchI GAGTC 2 cut(s) 277, 1747
SduI GDGCHC 4 cut(s) 106, 835, 1143, 1917
Sfr274I CTCGAG 2 cut(s) 504, 1480
SfuI TTCGAA 1 cut(s) 919
SinI GGWCC 1 cut(s) 945
SlaI CTCGAG 2 cut(s) 504, 1480
SmiMI CAYNNNNRTG 3 cut(s) 99, 384, 902
SmlI CTYRAG 7 cut(s) 504, 764, 1346, 1355, 1385, 1480, 2018
SmoI CTYRAG 7 cut(s) 504, 764, 1346, 1355, 1385, 1480, 2018
SphI GCATGC 1 cut(s) 1641
SsiI CCGC 4 cut(s) 513, 943, 1030, 2005
SspMI CTAG 6 cut(s) 864, 986, 1242, 1337, 1647, 1823
SstI GAGCTC 1 cut(s) 1143
StyI CCWWGG 2 cut(s) 46, 1873
TaaI ACNGT 4 cut(s) 43, 881, 1919, 1992
TaiI ACGT 5 cut(s) 360, 449, 704, 1180, 1440
TaqI TCGA 4 cut(s) 505, 556, 919, 1481
TatI WGTACW 3 cut(s) 334, 1659, 1827
TfiI GAWTC 5 cut(s) 197, 422, 467, 677, 1360
Tru1I TTAA 5 cut(s) 23, 1319, 1347, 1386, 1604
Tru9I TTAA 5 cut(s) 23, 1319, 1347, 1386, 1604
TscAI CASTG 4 cut(s) 46, 1447, 1896, 1922
TseFI GTSAC 6 cut(s) 37, 299, 549, 1178, 1438, 1801
TseI GCWGC 4 cut(s) 539, 542, 720, 1634
Tsp45I GTSAC 6 cut(s) 37, 299, 549, 1178, 1438, 1801
TspGWI ACGGA 2 cut(s) 1321, 1847
TspRI CASTG 4 cut(s) 46, 1447, 1896, 1922
Van91I CCANNNNNTGG 1 cut(s) 370
Vha464I CTTAAG 2 cut(s) 1346, 1385
VneI GTGCAC 1 cut(s) 102
VpaK11BI GGWCC 1 cut(s) 945
XapI RAATTY 8 cut(s) 177, 291, 599, 1146, 1297, 1391, 1514, 1679
XbaI TCTAGA 1 cut(s) 863
XceI RCATGY 3 cut(s) 98, 1134, 1641
XcmI CCANNNNNNNNNTGG 1 cut(s) 956
XhoI CTCGAG 2 cut(s) 504, 1480
XmiI GTMKAC 1 cut(s) 1174
XmnI GAANNNNTTC 4 cut(s) 177, 434, 1024, 1194
XspI CTAG 6 cut(s) 864, 986, 1242, 1337, 1647, 1823
ZraI GACGTC 3 cut(s) 358, 447, 1438
Zsp2I ATGCAT 1 cut(s) 1694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.