FvH4_6g52372

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000085
Physical Location & Seq
Reverse (-)
38591985 .. 38597622
5638 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g52372.t1

Sequence Viewer

Length: 3534 bp
ATGGACGGGAAAATGATTATTACGGTAATTGCTGCGTGGGTCAGTTACATGGCGATTAATAAGTGGATATGGACTCCAATGAAGCGGCGCCCAAATCCAATGCGGAAGGAAGATGATGATCCACCTTCTCCTCCTCGGCTGGAGTATGATGTGTTTTTAAGTTTCAGGGGTCGTGATACTCGCAAGGGTATTGCAAACCTTTTATACAAGGAATTGGAATCAAGTGGAATTAAAACGTTCATGGATGAGGAAGGGCTTAAAGTAGGGGCCGAAATTTCCTCAAATCTGCTGAAGGCAATCAAAGAATCGAGGTCTGCAATTGTTCTTCTCTCACCAAATTATGCTACTTCCCCATGGTGTTTGAATGAGCTTGCAAGCATTATTCAAGGCTTTGAAACCAGGAACACAACAGTTATTCCAATTTTCTATAATGTGGAAGCTTGTGACGTCCGACATCAAATGGGGAGTTTTAAAGAAGCCTTCATCAGACACGAACAAAGGAAAGAAGAGAAGGTGGAGCACTGGAAAGCTGCTCTAACCACAGTGGCCAACCTCTCAGGCTTGGAACTAAAAAACTATCGGTCTGAGAGACAGCTCGTTGAAGACATTGTGCAACGTGTGTACAGTATAGTACGAGATCTGGAAATTGAGTCTCCTGAAGATTTTGTAGAATATGAAGCAACAAGAAAAGCCATGGGTAAGATAATGAATGCGCTTAAGGATGATAGGTTCACTGCCATTGGTATCTATGGAACTGGAGGCATCGGTAAGACAACAATGGCAAGACATGTTGCTGCAGAAGCCAAAAGAAAAGGGATTTTTGATCATGTGGCGATGGCTGTTGTGACACACAAACCTGTCTTGAAAGACATTCAAGGCCAATTAGCAGTTCAGTTGGGTGTCAAATTGGATGAGAAAGAGACAATCCATGAAGGAACTAGTAAATTGAGTCAGGTGATAATGCGTAAAAATAAGATCCTTATAATCTTGGATGACATATGGAAGAAAACAGATTTGTCCCTCATTGGAATTCCTAGCTACAAGATGCTTGAAGATCGCAGTTCCAAAGTCCTCCTCACCACAAGGAATTCCAAGGTTTGCAGTTCCATGGATAGCCACCCAATTGCTCTCGATATGCTAAACCATGAAGATTCTTGGAACTTATTTGTGAAGAAAACAAGAATGTCTTCTGAAAAATTGGCCGTTGTCAATGAAGAAGCAAGTATTCTAGTGAAAGAGTGCGCTGGTCTCCCAATCGCGTTGGTTGCAATTGCTAGAGCACTTAGAGATGAAGATATCACGGAATGGAAACAAGCAGCTGAACGACTAAAGTCTTCTCAACGTAGAAACATTGATACTGTTGTGCTCAATTGTATTAAGCTAAGCTATGATCGATTGACATCTGAAGATGCCAAATTATGCTTCTTGCTTTGCTGCCTATTCCCAGAGGACTATGATATCCAAATTGAAGAGTTGATGATGTATGCATTCGGGAAAGGATTGTTCCGTGAGGGCAATACATTGCAAGAGGCCAGAATCGATTGCCATTCTGCAGTCAGGTCCCTTAAATCTTCTAGCTTGCTTTTGGGCTCTAAACATGATGGACATGTACGGATGCATGATGTCATCAGAAATGTTGCCAGAAAAATTTCGTCATCTGAAGATGGCCAATCTGTTTTCCATGGAGCTGGTCGTCAGTTTCAGACGTGGCCGAAGATCGAAAGAGACTACTGCGCAATCTCATTAATGATGAACAAAATCAGAAAGCTTCCCAAGAATTTGGTATGTTCGGAACTCAAGGTATTGTTGTTACAACACAACCCAAATGTCAGTACGATCCCGGAGTCTTTTCTTGGATGCCTGAGGGAGTTAAGCGTCCTAAATCTTAGCAAAACTAGTATTTCTGTGCTACCCCTATCATTTTCTCTCCTAACCAATCTTCACAGTTTGCATTTAGATTCTTGTGTGAAAATTTCCGACATATCTGTTCTCGGAAAACTTGAAAAGCTTGAGATTCTTAGTCTGATAGAATGTTGCCCTGAGGAAGTTCCAGAAGCAATAGGAAATTTAAGCAGGCTAAGAATGCTGGACATCAGTGAATCACCGGTTGTTAAAATTGCATCGCAAGTGATATCCCGTTTAAGTGGGTTAGAGGAACTTTATATGCAATGTGATTTTGTGGATTGGAGGAATGAAGTTGAGGAAGCAGGAAAAGAAACAAACATTGGCTTTGATGAGTTAACTGGCTCTTCATATTTACGCATTTTGAAGGTTGGGATACCTAATGCAATGGTCTTACCTAAGAATGTTGAAGACCATCCAGATTGGCTCTACTTTGATATATGTATCAGCAGTGACTCTGAAGTCAGAGAATCCACTTGGAAGCCACACTCCTCTCATGATTATTCAAGAACCTTGACTCTTAGCACACCGATTCATAACTTGCCGAAGTGGTTTACTGACGTGGTGACAGAGAAAGCAGAGAAACTACATTACAGAAAGTGCAAGGGCTTATATAACATTCTTGTGGAGTATGACTGTGGGAGATTAAATGAGTTGAGGTATCTCTCTATAATTGGGCCCAATGAGAATTTGAAGGAGTTGATGAACGCAGATGCAAGGGATTCAAATAATGCTGCATTCCCGAAGTTGGAAGAGTTACATCTCTTCAAGGTTGAAAACATCAACAACTTATGTGTTGGTAACTTACCTCCTGGGTCTCTATGCAAGCTCAAGTTATTGGATGTAAGGAAATGTCATAACTTGGTGAATGCTCTTTTACAATCACCTTTGTTGGAGAGACTGCAATTTCTGGAGAAGCTTCTTTGTGCACATCTGAATCGCTTGGAATATGTGTTTGGCCCTGGCAAAACTACTGCCTGTGAAAAGTTGAAAGAGATGAGACTGTGCAGATTACCAGAACTCATAAAGATATGGAGTGGTCAGGCTCCACATGCAATCTTCCGTAATCTTAAAATTTTGGCAGTTAGTGGGTGCAAGAAGCTGAAAAGTCTCTTCACGTCCGATATAGCTCATTGTCTATTGCAATTGGAAGACCTTTGGGTGCATTCATGCTTTAGCTTGGACCGAATTGTTGAACTGAGCGAGGAAACAGGGAAGAGGAATGACCTTTTTCCAAAATTACAGAACTTAGTCTTGGAAGATCTTCCTCTGCTCAGAAGGTTGTATAGTAACAGTAGCGGAGGTCCAACTTCATTGGAGATAGAGTTTCTCTCCTTAGAATACTTCTACAAGTTGGAATGTCCCCGGTTCTCCCCTCCTAATTTCCACAGCAATAAGCAAGTCCAAGTCAATGATATACAACATTATAGCGCTCTAAAGGAAAGGTTATGGGAGAACATGGAACTTGAGTTTACAATTTCTGAAGACGAGCGGCGTCGGTGGAAGTATGATGTATTGACACTGGCAGTATCACATCTGAACTATACGATAGACTGCAGCGGAGTGGGGTTTCTACCTTCATCGATGACTCAGGTATTCGAGGAGGGGACGCGATTTCTCTCACTCTCCTAG

Protein Analysis

1178

Amino Acids

134.05

Weight (kDa)

6.71

Isoelectric Point (pI)

46.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 49 - 209 2.5e-50 TIR domain
TIR_2 PF13676 51 - 163 1.3e-17 TIR domain
NB-ARC PF00931 230 - 395 6e-30 NB-ARC domain
LRR_14 PF23598 598 - 722 2.4e-12 Leucine-rich repeat region
LRR_RPS2 PF23247 884 - 1025 2.6e-22 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 977 - 1073 5.5e-15 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 983
AasI GACNNNNNNGTC 1 cut(s) 2363
AatII GACGTC 1 cut(s) 450
Acc16I TGCGCA 1 cut(s) 1735
AccB1I GGYRCC 1 cut(s) 87
AccBSI CCGCTC 1 cut(s) 3394
AccII CGCG 2 cut(s) 1259, 3514
AciI CCGC 5 cut(s) 85, 103, 3201, 3394, 3462
AclI AACGTT 1 cut(s) 236
AclWI GGATC 3 cut(s) 113, 970, 1831
AcoI YGGCCR 4 cut(s) 546, 1200, 1666, 1709
AcsI RAATTY 9 cut(s) 273, 1029, 1087, 1647, 1777, 1971, 2065, 2590, 2976
AcuI CTGAAG 6 cut(s) 311, 678, 1425, 1680, 2382, 3405
AcyI GRCGYC 3 cut(s) 88, 447, 3397
AfaI GTAC 4 cut(s) 623, 633, 1611, 1834
AfeI AGCGCT 1 cut(s) 3334
AfiI CCNNNNNNNGG 1 cut(s) 2650
AflII CTTAAG 1 cut(s) 716
AflIII ACRYGT 3 cut(s) 616, 787, 1606
AgeI ACCGGT 1 cut(s) 2104
AhlI ACTAGT 2 cut(s) 938, 1895
AjiI CACGTC 3 cut(s) 1707, 2464, 3021
AjnI CCWGG 3 cut(s) 398, 2713, 2863
AloI GAACNNNNNNTCC 2 cut(s) 713, 745
Alw21I GWGCWC 4 cut(s) 522, 1282, 1368, 2833
Alw26I GTCTC 9 cut(s) 583, 657, 914, 1253, 1719, 2724, 2794, 2896, 3017
Alw44I GTGCAC 1 cut(s) 2829
AlwI GGATC 3 cut(s) 113, 970, 1831
Aor51HI AGCGCT 1 cut(s) 3334
AoxI GGCC 9 cut(s) 267, 546, 877, 1200, 1530, 1666, 1709, 2579, 2860
ApaI GGGCCC 1 cut(s) 2583
ApaLI GTGCAC 1 cut(s) 2829
ApeKI GCWGC 7 cut(s) 32, 530, 794, 1316, 1434, 2636, 3459
ApoI RAATTY 9 cut(s) 273, 1029, 1087, 1647, 1777, 1971, 2065, 2590, 2976
AseI ATTAAT 2 cut(s) 57, 1745
AsiGI ACCGGT 1 cut(s) 2104
Asp700I GAANNNNTTC 3 cut(s) 1186, 1648, 3165
AspLEI GCGC 5 cut(s) 90, 715, 1244, 1736, 3335
AspS9I GGNCC 7 cut(s) 267, 1560, 2579, 2580, 2861, 3085, 3206
AsuC2I CCSGG 2 cut(s) 1841, 3268
AsuHPI GGTGA 7 cut(s) 324, 967, 1069, 2094, 2479, 2778, 2779
AvaII GGWCC 3 cut(s) 1560, 3085, 3206
AxyI CCTNAGG 2 cut(s) 1862, 2040
BaeGI GKGCMC 2 cut(s) 2583, 2833
BaeI ACNNNNGTAYC 2 cut(s) 1347, 1380
BalI TGGCCA 2 cut(s) 548, 1668
BanI GGYRCC 1 cut(s) 87
BanII GRGCYC 2 cut(s) 1592, 2583
BbsI GAAGAC 6 cut(s) 609, 1179, 1326, 2319, 3060, 3393
Bbv12I GWGCWC 4 cut(s) 522, 1282, 1368, 2833
BbvI GCAGC 7 cut(s) 19, 517, 781, 1328, 1421, 2623, 3471
BccI CCATC 4 cut(s) 829, 1595, 1658, 2325
BceAI ACGGC 1 cut(s) 1187
BciT130I CCWGG 3 cut(s) 400, 2715, 2865
BciVI GTATCC 1 cut(s) 2271
BclI TGATCA 1 cut(s) 823
BcnI CCSGG 2 cut(s) 1841, 3268
BcoDI GTCTC 9 cut(s) 583, 657, 914, 1253, 1719, 2724, 2794, 2896, 3017
BcuI ACTAGT 2 cut(s) 938, 1895
BfaI CTAG 7 cut(s) 939, 1035, 1229, 1275, 1575, 1896, 3532
BfmI CTRYAG 3 cut(s) 795, 1551, 3457
BfoI RGCGCY 2 cut(s) 91, 3336
BfrI CTTAAG 1 cut(s) 716
BfuI GTATCC 1 cut(s) 2271
BglII AGATCT 2 cut(s) 637, 3163
BisI GCNGC 9 cut(s) 33, 86, 531, 795, 1317, 1435, 2637, 3395, 3460
BlpI GCTNAGC 1 cut(s) 1382
BlsI GCNGC 9 cut(s) 34, 87, 532, 796, 1318, 1436, 2638, 3396, 3461
Bme1390I CCNGG 5 cut(s) 400, 1841, 2715, 2865, 3268
Bme18I GGWCC 3 cut(s) 1560, 3085, 3206
BmgBI CACGTC 3 cut(s) 1707, 2464, 3021
BmgT120I GGNCC 7 cut(s) 267, 1560, 2579, 2580, 2861, 3085, 3206
BmiI GGNNCC 5 cut(s) 89, 268, 1562, 2581, 2949
BmrFI CCNGG 5 cut(s) 400, 1841, 2715, 2865, 3268
BmsI GCATC 7 cut(s) 771, 1035, 1399, 1605, 1847, 2129, 2605
BoxI GACNNNNGTC 1 cut(s) 1330
BpiI GAAGAC 6 cut(s) 609, 1179, 1326, 2319, 3060, 3393
BplI GAGNNNNNCTC 2 cut(s) 3218, 3250
BpmI CTGGAG 3 cut(s) 161, 777, 2834
Bpu1102I GCTNAGC 1 cut(s) 1382
BpuEI CTTGAG 4 cut(s) 1781, 2030, 2717, 3389
BpuMI CCSGG 2 cut(s) 1841, 3268
Bsa29I ATCGAT 3 cut(s) 1393, 1539, 3485
BsaBI GATNNNNATC 2 cut(s) 117, 1399
BsaHI GRCGYC 3 cut(s) 88, 447, 3397
BsaI GGTCTC 2 cut(s) 1253, 2724
BsaJI CCNNGG 9 cut(s) 134, 353, 693, 1092, 1107, 1681, 2714, 2863, 3266
BsaWI WCCGGW 1 cut(s) 2104
BsaXI ACNNNNNCTCC 2 cut(s) 3212, 3242
Bsc4I CCNNNNNNNGG 1 cut(s) 2650
Bse118I RCCGGY 1 cut(s) 2104
Bse1I ACTGG 4 cut(s) 527, 760, 2248, 3429
Bse21I CCTNAGG 2 cut(s) 1862, 2040
Bse3DI GCAATG 3 cut(s) 1520, 2174, 2295
Bse8I GATNNNNATC 2 cut(s) 117, 1399
BseBI CCWGG 3 cut(s) 400, 2715, 2865
BseCI ATCGAT 3 cut(s) 1393, 1539, 3485
BseDI CCNNGG 9 cut(s) 134, 353, 693, 1092, 1107, 1681, 2714, 2863, 3266
BseGI GGATG 8 cut(s) 250, 727, 916, 997, 1620, 1862, 2317, 2749
BseJI GATNNNNATC 2 cut(s) 117, 1399
BseLI CCNNNNNNNGG 1 cut(s) 2650
BseMI GCAATG 3 cut(s) 1520, 2174, 2295
BseMII CTCAG 7 cut(s) 570, 576, 1853, 2031, 3092, 3190, 3506
BseNI ACTGG 4 cut(s) 527, 760, 2248, 3429
BseRI GAGGAG 5 cut(s) 120, 123, 1064, 2383, 3518
BseSI GKGCMC 2 cut(s) 2583, 2833
BseXI GCAGC 7 cut(s) 19, 517, 781, 1328, 1421, 2623, 3471
BsgI GTGCAG 1 cut(s) 2929
Bsh1236I CGCG 2 cut(s) 1259, 3514
BshFI GGCC 9 cut(s) 269, 548, 879, 1202, 1532, 1668, 1711, 2581, 2862
BshNI GGYRCC 1 cut(s) 87
BshTI ACCGGT 1 cut(s) 2104
BshVI ATCGAT 3 cut(s) 1393, 1539, 3485
BsiHKAI GWGCWC 4 cut(s) 522, 1282, 1368, 2833
BsiSI CCGG 3 cut(s) 1841, 2105, 3268
BslFI GGGAC 4 cut(s) 1003, 1546, 3249, 3523
BslI CCNNNNNNNGG 1 cut(s) 2650
BsmAI GTCTC 9 cut(s) 583, 657, 914, 1253, 1719, 2724, 2794, 2896, 3017
BsmFI GGGAC 4 cut(s) 1003, 1546, 3249, 3523
BsmI GAATGC 6 cut(s) 715, 1487, 2088, 2639, 2776, 3067
BsnI GGCC 9 cut(s) 269, 548, 879, 1202, 1532, 1668, 1711, 2581, 2862
Bso31I GGTCTC 2 cut(s) 1253, 2724
Bsp120I GGGCCC 1 cut(s) 2579
Bsp1286I GDGCHC 6 cut(s) 522, 1282, 1368, 1592, 2583, 2833
Bsp1407I TGTACA 1 cut(s) 621
Bsp143I GATC 9 cut(s) 118, 637, 823, 975, 1054, 1390, 1716, 1836, 3163
Bsp1720I GCTNAGC 1 cut(s) 1382
Bsp19I CCATGG 4 cut(s) 353, 693, 1107, 1681
BspACI CCGC 5 cut(s) 85, 103, 3201, 3394, 3462
BspANI GGCC 9 cut(s) 269, 548, 879, 1202, 1532, 1668, 1711, 2581, 2862
BspCNI CTCAG 7 cut(s) 569, 577, 1854, 2032, 3093, 3189, 3505
BspDI ATCGAT 3 cut(s) 1393, 1539, 3485
BspFNI CGCG 2 cut(s) 1259, 3514
BspHI TCATGA 1 cut(s) 2398
BspLI GGNNCC 5 cut(s) 89, 268, 1562, 2581, 2949
BspMAI CTGCAG 3 cut(s) 799, 1555, 3461
BspPI GGATC 3 cut(s) 113, 970, 1831
BspQI GCTCTTC 1 cut(s) 2254
BspT107I GGYRCC 1 cut(s) 87
BspTI CTTAAG 1 cut(s) 716
BspTNI GGTCTC 2 cut(s) 1253, 2724
BsrBI CCGCTC 1 cut(s) 3394
BsrDI GCAATG 3 cut(s) 1520, 2174, 2295
BsrFI RCCGGY 1 cut(s) 2104
BsrGI TGTACA 1 cut(s) 621
BsrI ACTGG 4 cut(s) 527, 760, 2248, 3429
BssAI RCCGGY 1 cut(s) 2104
BssECI CCNNGG 9 cut(s) 134, 353, 693, 1092, 1107, 1681, 2714, 2863, 3266
BssMI GATC 9 cut(s) 118, 637, 823, 975, 1054, 1390, 1716, 1836, 3163
BssNI GRCGYC 3 cut(s) 88, 447, 3397
BssT1I CCWWGG 5 cut(s) 353, 693, 1092, 1107, 1681
Bst2UI CCWGG 3 cut(s) 400, 2715, 2865
Bst4CI ACNGT 9 cut(s) 25, 412, 544, 626, 1360, 1946, 2540, 2907, 3197
Bst6I CTCTTC 7 cut(s) 501, 1464, 2254, 2649, 2672, 3020, 3113
BstACI GRCGYC 3 cut(s) 88, 447, 3397
BstAFI CTTAAG 1 cut(s) 716
BstAUI TGTACA 1 cut(s) 621
BstC8I GCNNGC 5 cut(s) 372, 376, 1580, 2075, 2729
BstDSI CCRYGG 4 cut(s) 353, 693, 1107, 1681
BstF5I GGATG 8 cut(s) 250, 727, 916, 997, 1620, 1862, 2317, 2749
BstFNI CGCG 2 cut(s) 1259, 3514
BstH2I RGCGCY 2 cut(s) 91, 3336
BstHHI GCGC 5 cut(s) 90, 715, 1244, 1736, 3335
BstKTI GATC 9 cut(s) 121, 640, 826, 978, 1057, 1393, 1719, 1839, 3166
BstMAI GTCTC 9 cut(s) 583, 657, 914, 1253, 1719, 2724, 2794, 2896, 3017
BstMBI GATC 9 cut(s) 118, 637, 823, 975, 1054, 1390, 1716, 1836, 3163
BstMWI GCNNNNNNNGC 4 cut(s) 800, 1265, 2083, 2954
BstNI CCWGG 3 cut(s) 400, 2715, 2865
BstNSI RCATGY 3 cut(s) 791, 1610, 2957
BstPAI GACNNNNGTC 1 cut(s) 1330
BstSCI CCNGG 5 cut(s) 398, 1839, 2713, 2863, 3266
BstSFI CTRYAG 3 cut(s) 795, 1551, 3457
BstSLI GKGCMC 2 cut(s) 2583, 2833
BstUI CGCG 2 cut(s) 1259, 3514
BstV1I GCAGC 7 cut(s) 19, 517, 781, 1328, 1421, 2623, 3471
BstV2I GAAGAC 6 cut(s) 609, 1179, 1326, 2319, 3060, 3393
BstX2I RGATCY 3 cut(s) 637, 975, 3163
BstXI CCANNNNNNTGG 2 cut(s) 1688, 1780
BstYI RGATCY 3 cut(s) 637, 975, 3163
Bsu15I ATCGAT 3 cut(s) 1393, 1539, 3485
Bsu36I CCTNAGG 2 cut(s) 1862, 2040
BsuI GTATCC 1 cut(s) 2271
BsuRI GGCC 9 cut(s) 269, 548, 879, 1202, 1532, 1668, 1711, 2581, 2862
BsuTUI ATCGAT 3 cut(s) 1393, 1539, 3485
BtgI CCRYGG 4 cut(s) 353, 693, 1107, 1681
BtgZI GCGATG 2 cut(s) 848, 2106
BtrI CACGTC 3 cut(s) 1707, 2464, 3021
BtsCI GGATG 8 cut(s) 250, 727, 916, 997, 1620, 1862, 2317, 2749
BtsI GCAGTG 2 cut(s) 732, 2359
BtsIMutI CAGTG 6 cut(s) 520, 549, 732, 2101, 2359, 3422
Cac8I GCNNGC 5 cut(s) 372, 376, 1580, 2075, 2729
CciI TCATGA 1 cut(s) 2398
CfoI GCGC 5 cut(s) 90, 715, 1244, 1736, 3335
Cfr10I RCCGGY 1 cut(s) 2104
Cfr13I GGNCC 7 cut(s) 267, 1560, 2579, 2580, 2861, 3085, 3206
ClaI ATCGAT 3 cut(s) 1393, 1539, 3485
CseI GACGC 3 cut(s) 1864, 3386, 3520
Csp6I GTAC 4 cut(s) 622, 632, 1610, 1833
CspAI ACCGGT 1 cut(s) 2104
CviQI GTAC 4 cut(s) 622, 632, 1610, 1833
DinI GGCGCC 1 cut(s) 89
DpnI GATC 9 cut(s) 120, 639, 825, 977, 1056, 1392, 1718, 1838, 3165
DpnII GATC 9 cut(s) 118, 637, 823, 975, 1054, 1390, 1716, 1836, 3163
DraI TTTAAA 1 cut(s) 472
DrdI GACNNNNNNGTC 1 cut(s) 2363
DseDI GACNNNNNNGTC 1 cut(s) 2363
EaeI YGGCCR 4 cut(s) 546, 1200, 1666, 1709
Eam1104I CTCTTC 7 cut(s) 501, 1464, 2254, 2649, 2672, 3020, 3113
EarI CTCTTC 7 cut(s) 501, 1464, 2254, 2649, 2672, 3020, 3113
Eco130I CCWWGG 5 cut(s) 353, 693, 1092, 1107, 1681
Eco24I GRGCYC 2 cut(s) 1592, 2583
Eco31I GGTCTC 2 cut(s) 1253, 2724
Eco32I GATATC 3 cut(s) 1297, 1459, 2133
Eco47I GGWCC 3 cut(s) 1560, 3085, 3206
Eco47III AGCGCT 1 cut(s) 3334
Eco57I CTGAAG 6 cut(s) 311, 678, 1425, 1680, 2382, 3405
Eco81I CCTNAGG 2 cut(s) 1862, 2040
EcoO109I RGGNCCY 1 cut(s) 1560
EcoRI GAATTC 2 cut(s) 1029, 1087
EcoRII CCWGG 3 cut(s) 398, 2713, 2863
EcoRV GATATC 3 cut(s) 1297, 1459, 2133
EcoT14I CCWWGG 5 cut(s) 353, 693, 1092, 1107, 1681
EcoT22I ATGCAT 2 cut(s) 1489, 1620
EcoT38I GRGCYC 2 cut(s) 1592, 2583
EgeI GGCGCC 1 cut(s) 89
EheI GGCGCC 1 cut(s) 89
ErhI CCWWGG 5 cut(s) 353, 693, 1092, 1107, 1681
FalI AAGNNNNNCTT 2 cut(s) 1171, 1203
FaqI GGGAC 4 cut(s) 1003, 1546, 3249, 3523
FauNDI CATATG 1 cut(s) 998
FbaI TGATCA 1 cut(s) 823
Fnu4HI GCNGC 9 cut(s) 33, 86, 531, 795, 1317, 1435, 2637, 3395, 3460
FokI GGATG 8 cut(s) 257, 734, 923, 1004, 1627, 1869, 2304, 2756
FriOI GRGCYC 2 cut(s) 1592, 2583
Fsp4HI GCNGC 9 cut(s) 33, 86, 531, 795, 1317, 1435, 2637, 3395, 3460
FspBI CTAG 7 cut(s) 939, 1035, 1229, 1275, 1575, 1896, 3532
FspI TGCGCA 1 cut(s) 1735
GlaI GCGC 5 cut(s) 89, 714, 1243, 1735, 3334
GluI GCNGC 9 cut(s) 33, 86, 531, 795, 1317, 1435, 2637, 3395, 3460
GsuI CTGGAG 3 cut(s) 161, 777, 2834
HaeII RGCGCY 2 cut(s) 91, 3336
HaeIII GGCC 9 cut(s) 269, 548, 879, 1202, 1532, 1668, 1711, 2581, 2862
HapII CCGG 3 cut(s) 1841, 2105, 3268
HgaI GACGC 3 cut(s) 1864, 3386, 3520
HhaI GCGC 5 cut(s) 90, 715, 1244, 1736, 3335
Hin1I GRCGYC 3 cut(s) 88, 447, 3397
Hin6I GCGC 5 cut(s) 88, 713, 1242, 1734, 3333
HinP1I GCGC 5 cut(s) 88, 713, 1242, 1734, 3333
HincII GTYRAC 1 cut(s) 2241
HindII GTYRAC 1 cut(s) 2241
HindIII AAGCTT 4 cut(s) 438, 1766, 2006, 2819
HpaI GTTAAC 1 cut(s) 2241
HpaII CCGG 3 cut(s) 1841, 2105, 3268
HphI GGTGA 7 cut(s) 324, 967, 1069, 2094, 2479, 2778, 2779
Hpy166II GTNNAC 6 cut(s) 622, 732, 2241, 2457, 2831, 3375
Hpy8I GTNNAC 6 cut(s) 622, 732, 2241, 2457, 2831, 3375
Hpy99I CGWCG 1 cut(s) 3402
HpyCH4III ACNGT 9 cut(s) 25, 412, 544, 626, 1360, 1946, 2540, 2907, 3197
HpyCH4IV ACGT 7 cut(s) 236, 447, 616, 1342, 1706, 2463, 3020
HpyF10VI GCNNNNNNNGC 4 cut(s) 800, 1265, 2083, 2954
HpySE526I ACGT 7 cut(s) 236, 447, 616, 1342, 1706, 2463, 3020
Hsp92I GRCGYC 3 cut(s) 88, 447, 3397
HspAI GCGC 5 cut(s) 88, 713, 1242, 1734, 3333
KasI GGCGCC 1 cut(s) 87
Ksp22I TGATCA 1 cut(s) 823
KspAI GTTAAC 1 cut(s) 2241
Kzo9I GATC 9 cut(s) 118, 637, 823, 975, 1054, 1390, 1716, 1836, 3163
LguI GCTCTTC 1 cut(s) 2254
LmnI GCTCC 3 cut(s) 517, 1685, 2953
Lsp1109I GCAGC 7 cut(s) 19, 517, 781, 1328, 1421, 2623, 3471
LweI GCATC 7 cut(s) 771, 1035, 1399, 1605, 1847, 2129, 2605
MaeI CTAG 7 cut(s) 939, 1035, 1229, 1275, 1575, 1896, 3532
MaeII ACGT 7 cut(s) 236, 447, 616, 1342, 1706, 2463, 3020
MaeIII GTNAC 9 cut(s) 44, 443, 844, 1809, 2354, 2467, 2658, 2702, 3191
MalI GATC 9 cut(s) 120, 639, 825, 977, 1056, 1392, 1718, 1838, 3165
MbiI CCGCTC 1 cut(s) 3394
MboI GATC 9 cut(s) 118, 637, 823, 975, 1054, 1390, 1716, 1836, 3163
MfeI CAATTG 5 cut(s) 318, 1122, 1269, 1369, 3047
MflI RGATCY 3 cut(s) 637, 975, 3163
MhlI GDGCHC 6 cut(s) 522, 1282, 1368, 1592, 2583, 2833
MlsI TGGCCA 2 cut(s) 548, 1668
MluNI TGGCCA 2 cut(s) 548, 1668
Mly113I GGCGCC 1 cut(s) 88
MlyI GAGTC 7 cut(s) 67, 659, 958, 1853, 2351, 2413, 3484
MmeI TCCRAC 6 cut(s) 475, 2001, 2631, 2775, 3233, 3237
Mox20I TGGCCA 2 cut(s) 548, 1668
Mph1103I ATGCAT 2 cut(s) 1489, 1620
MroXI GAANNNNTTC 3 cut(s) 1186, 1648, 3165
MscI TGGCCA 2 cut(s) 548, 1668
MslI CAYNNNNRTG 1 cut(s) 2525
Msp20I TGGCCA 2 cut(s) 548, 1668
MspA1I CMGCKG 2 cut(s) 1319, 3462
MspCI CTTAAG 1 cut(s) 716
MspI CCGG 3 cut(s) 1841, 2105, 3268
MspR9I CCNGG 5 cut(s) 400, 1841, 2715, 2865, 3268
MunI CAATTG 5 cut(s) 318, 1122, 1269, 1369, 3047
Mva1269I GAATGC 6 cut(s) 715, 1487, 2088, 2639, 2776, 3067
MvaI CCWGG 3 cut(s) 400, 2715, 2865
MvnI CGCG 2 cut(s) 1259, 3514
MwoI GCNNNNNNNGC 4 cut(s) 800, 1265, 2083, 2954
NarI GGCGCC 1 cut(s) 88
NciI CCSGG 2 cut(s) 1841, 3268
NcoI CCATGG 4 cut(s) 353, 693, 1107, 1681
NdeI CATATG 1 cut(s) 998
NdeII GATC 9 cut(s) 118, 637, 823, 975, 1054, 1390, 1716, 1836, 3163
NlaIV GGNNCC 5 cut(s) 89, 268, 1562, 2581, 2949
NmeAIII GCCGAG 1 cut(s) 115
NmuCI GTSAC 4 cut(s) 443, 844, 2354, 2467
NsbI TGCGCA 1 cut(s) 1735
NsiI ATGCAT 2 cut(s) 1489, 1620
NspI RCATGY 3 cut(s) 791, 1610, 2957
PagI TCATGA 1 cut(s) 2398
PciI ACATGT 2 cut(s) 787, 1606
PciSI GCTCTTC 1 cut(s) 2254
PctI GAATGC 6 cut(s) 715, 1487, 2088, 2639, 2776, 3067
PdmI GAANNNNTTC 3 cut(s) 1186, 1648, 3165
PfoI TCCNGGA 1 cut(s) 1839
PinAI ACCGGT 1 cut(s) 2104
PkrI GCNGC 9 cut(s) 34, 87, 532, 796, 1318, 1436, 2638, 3396, 3461
PleI GAGTC 7 cut(s) 67, 658, 957, 1852, 2351, 2413, 3484
PluTI GGCGCC 1 cut(s) 91
PpsI GAGTC 7 cut(s) 67, 658, 957, 1852, 2351, 2413, 3484
PpuMI RGGWCCY 1 cut(s) 1560
PscI ACATGT 2 cut(s) 787, 1606
PshAI GACNNNNGTC 1 cut(s) 1330
PshBI ATTAAT 2 cut(s) 57, 1745
PsiI TTATAA 1 cut(s) 983
Psp1406I AACGTT 1 cut(s) 236
Psp5II RGGWCCY 1 cut(s) 1560
Psp6I CCWGG 3 cut(s) 398, 2713, 2863
PspGI CCWGG 3 cut(s) 398, 2713, 2863
PspN4I GGNNCC 5 cut(s) 89, 268, 1562, 2581, 2949
PspOMI GGGCCC 1 cut(s) 2579
PspPI GGNCC 7 cut(s) 267, 1560, 2579, 2580, 2861, 3085, 3206
PspPPI RGGWCCY 1 cut(s) 1560
PstI CTGCAG 3 cut(s) 799, 1555, 3461
PsuI RGATCY 3 cut(s) 637, 975, 3163
PvuII CAGCTG 1 cut(s) 1319
RsaI GTAC 4 cut(s) 623, 633, 1611, 1834
RsaNI GTAC 4 cut(s) 622, 632, 1610, 1833
RseI CAYNNNNRTG 1 cut(s) 2525
SapI GCTCTTC 1 cut(s) 2254
SatI GCNGC 9 cut(s) 33, 86, 531, 795, 1317, 1435, 2637, 3395, 3460
Sau3AI GATC 9 cut(s) 118, 637, 823, 975, 1054, 1390, 1716, 1836, 3163
Sau96I GGNCC 7 cut(s) 267, 1560, 2579, 2580, 2861, 3085, 3206
SchI GAGTC 7 cut(s) 67, 659, 958, 1853, 2351, 2413, 3484
ScrFI CCNGG 5 cut(s) 400, 1841, 2715, 2865, 3268
SduI GDGCHC 6 cut(s) 522, 1282, 1368, 1592, 2583, 2833
SfaNI GCATC 7 cut(s) 771, 1035, 1399, 1605, 1847, 2129, 2605
SfcI CTRYAG 3 cut(s) 795, 1551, 3457
SfoI GGCGCC 1 cut(s) 89
SinI GGWCC 3 cut(s) 1560, 3085, 3206
SmiMI CAYNNNNRTG 1 cut(s) 2525
SmlI CTYRAG 5 cut(s) 716, 1796, 2009, 2732, 3368
SmoI CTYRAG 5 cut(s) 716, 1796, 2009, 2732, 3368
SpeI ACTAGT 2 cut(s) 938, 1895
SsiI CCGC 5 cut(s) 85, 103, 3201, 3394, 3462
SspDI GGCGCC 1 cut(s) 87
SspMI CTAG 7 cut(s) 939, 1035, 1229, 1275, 1575, 1896, 3532
StyD4I CCNGG 5 cut(s) 398, 1839, 2713, 2863, 3266
StyI CCWWGG 5 cut(s) 353, 693, 1092, 1107, 1681
TaaI ACNGT 9 cut(s) 25, 412, 544, 626, 1360, 1946, 2540, 2907, 3197
TaiI ACGT 7 cut(s) 239, 450, 619, 1345, 1709, 2466, 3023
TaqI TCGA 7 cut(s) 308, 1131, 1393, 1539, 1719, 3485, 3501
TaqII GACCGA 2 cut(s) 570, 3102
TatI WGTACW 1 cut(s) 621
TauI GCSGC 2 cut(s) 88, 3397
TscAI CASTG 6 cut(s) 527, 549, 739, 2101, 2359, 3429
TseFI GTSAC 4 cut(s) 443, 844, 2354, 2467
TseI GCWGC 7 cut(s) 32, 530, 794, 1316, 1434, 2636, 3459
Tsp45I GTSAC 4 cut(s) 443, 844, 2354, 2467
TspGWI ACGGA 4 cut(s) 1316, 1496, 1627, 2954
TspRI CASTG 6 cut(s) 527, 549, 739, 2101, 2359, 3429
Vha464I CTTAAG 1 cut(s) 716
VneI GTGCAC 1 cut(s) 2829
VpaK11BI GGWCC 3 cut(s) 1560, 3085, 3206
VspI ATTAAT 2 cut(s) 57, 1745
XapI RAATTY 9 cut(s) 273, 1029, 1087, 1647, 1777, 1971, 2065, 2590, 2976
XceI RCATGY 3 cut(s) 791, 1610, 2957
XmnI GAANNNNTTC 3 cut(s) 1186, 1648, 3165
XspI CTAG 7 cut(s) 939, 1035, 1229, 1275, 1575, 1896, 3532
ZraI GACGTC 1 cut(s) 448
Zsp2I ATGCAT 2 cut(s) 1489, 1620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.