Rroxscaffold_1G00019390

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000085
Physical Location & Seq
Forward (+)
23697895 .. 23707232
9338 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00019390.1

Sequence Viewer

Length: 3009 bp
ATGAAGGGTAAGGACACAATTCTCCCAATTTTTTATAAGTTGGATCCCGCTGTTGTACGATATCAAAAGAAAGAGACAAATTTTGGAAGAGCTTTCACTAAGCATGAAGCTAGACAAGAGAATGAGAAGATGAACCAGTGGAGAGCTGCTTTAAAAGGGGTGGCTGAAATCTCTGGGTGGCATTCAACCAACTATCCGACGGATATAGAACTAATTGAAGACATTGTGAAACATTTGTGGGAGAAAGTACTTGAAGCACCCACCGGAAATTTTGAAACATTTGAAGCGACAAGGCAAGCCATGGATGAGGTTATGAAGGTATTAAAAGATGATCAAGTCACTGTCCTTGGGGTGCATGGAGTGGGTGGCGTTGGAAAGACGACGATGGTCAAACATGTTGGTGCACAATCTAAGAAAGATGGGCTTTTTGATCATGTGATCATGGTTGTCGTATCCAAAAACCCTGACTTGGAAGAAATTCAAGGCACATTGGCAGAATCGCTGAGCTTGAAGGAGGGTATCGTGAGAGGAAATAGGATGCTTATAATCTTGGATGACATTTGGGAGAGTCTTGAACTCTCGAGCATTGGAATTCCCAGTCACAATGAGCTTCAAAGGTGCAATTCTAAAGTTATACTCACCACAAGGAAAAGCAATGTATGCCATTCTATGGGGTGCCATGCCCAGATCCCTCTCAATGTTCTATCAGAGGAAGATTCTTGGAAGTTATTCATGGAGACGTCGGGGAAGTCTTTTCATGAATCTACCGTTTCCTATAATGAAGCGAGAAAGGTAGCTGGAGAATGCGGTGGTCTCCCAAAAGCAATAAAAGCAGCTGCAAGGTCAATTGGAGATAAATATTTGGACGAATGGAATGAAGCAGTTGCAAGGGCATTTGGAGATGACCGCTTGGACGAATGGAATGAGACGGCTCAACCTGTCAACTCCGAAGCTGAGGAAAACGCATTTCATTCATTGGCATCTGATGATGCCAAGTCATGCTTCTTGTTATGCTGCCTATTCCCAGAAAATTATGATATCCCAATTGAAGATCTGCTAAAGTATGGGATTGGAAAAGGTTTGTTTCAATATGATACAATGCAAGAAGCCAGAGATAGAGCACATTCTGTGGTCAAGTACCTCAAAGCTTCTAGATTGCTTTTGGACGGTACAAAGAAGGGATGCATAAGGATGACTGATGACATTCGAAATCTGGCCATGTCCATTGCGTTGTCCGAAGAAGGTGATGGGTTTTTAGTAAAAGCTGGTTGTGAACTAGAGGAGTGGCCAGAGGCAGCATGGTGGCAAGGATTTACTTCCTGGCTCCGCTACAGCTGGGATTGGCCAATAGATGCTCATGAAGGATACTCAACAATCTCACTCATGAAAAATAAGCTACATAAGCTTCCCGAATATTTGGTATGTTCAAAGCTCCAAATTTTATTGTTACAAAGTAATGCTGGTATATCTGACATCCCAGAAACCTTTTTGCAAAGTCCTAATGCGCTAAGGGTCTTTGATCTTAGCTTCACTAGTATTTCATCACTGCCTCCATCTTTCAGTTTCCTAACCAACATCCAAGTTTTGAATTTGGATTTTTGCCATAAGTTAAGTAACATATCCGTTCTAGGAAAACTTAAGAAACTTGAGATTCTTAGTATGAGAGAACTTCCTCTTAAGAAATTTCCGAAACAAATAGGAGAATTGACCAGTCTAAGGAAGTTGGACGTCACTGGTAGATATCTTGAAACAATCCCATCTAAAGTGATATCGAAGTTGTATAGATTGGAAGAACTGCACATGAAATTTCAATTTGGGAAGTGGGGAAGTAAAATTGAGGATGCAGTTGAAGAAACCAATGCTGACTTTGATGAGTTGACTGGCTTGTTGGATTTAAACATTTTGGAAGTTTACATATCTGATGCTGCATGCTTGCCTGAAAGTATTGAGTACTTCAAGCCGAATTGGGTAAAGTTTGATATATGCATCAGCAGACGCGTCCCTTACGTGCTGTTCCCTCAACATGATGGTCTTTCAAGAACCTTGACTCTTGACACAACAATCAATAGCTTACCGGATTGGTTTATCCGCGTTGTGGCAGAGAAAGTGGAGAAGCTAGAGTACACAAATTGCGAAGACTTGAATGATGTCGTTGGGGAATATGACCATGGCAGATTACTTGCACTGAAATATCTCTCTGTGAACGGGAACCGTAAGAACTTGAAAGAGATAATCAACACAATGACACGGGTTTCAAATAAGCCAGTATTTGAGAACTTGGAAGAGTTGCATCTGTACTGCTTATATTCCCTCAATGAGTTATGTGTTGGTGAGCTACCACCTGGGTCTCTACGCAAGCTCAAGTTCTTGAAAGTGCAAACTTGTCGTAGCTTGGTGAATGCACTTTTACCATCAACATTGTTGCAGAGACTACAAAATCTGGAAGAAATCATTTGTATAAATCTGGAAGGAATGAAATATGTATTTGGATCTGATGAAGGGCTTGATCCAGAAAAGATTTTCTTGAGGAAATTGAGGGAGATGAGATTGTCTGCTCTATACAAACTAGTAAGCATATGTAATGGTCCTGCTACGTGTGCAATCTTCCAGAATCTTAAAGTTTTGGCAATTTGCAAGTGCAAGGAATTGAAAAATCTCTTCACAGTAGATGTGGCTGAATGTCTTTTTCAGTTGGAAGACCTTTCCATAGAGCGCTGTCCTAGCTTGGACAGAGTCATTGAAGCAAGCTATAGCTTGAACAGCAAGATTAAACTTCCCACGTTGAAGAAGTTAGCTTTGATAGATCTTCCGGCGCTCACAAGGTTCTGCACTGAAAGTGGTACTATTGAAACTGAGTGCCCTTCATTGGAATACTTGTACGTGGAGAAGTGCCTCCAGTTTTCATATTTTTCTTACCACTTCGACAACAAGAACCAAGTCCACTTCAATGATAAACAACATTTGAGTTCTTTAGTGAAAAGGTGGGAGGAAGTGCATAATCGGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1002

Amino Acids

114.05

Weight (kDa)

5.73

Isoelectric Point (pI)

37.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 4 - 88 9.6e-14 TIR domain
NB-ARC PF00931 99 - 248 4.9e-27 NB-ARC domain
LRR_8 PF13855 477 - 535 3.8e-07 Leucine rich repeat
LRR_14 PF23598 506 - 602 1.5e-10 Leucine-rich repeat region
LRR_RPS2 PF23247 760 - 906 9.1e-25 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 36, 545
AatII GACGTC 2 cut(s) 743, 1731
AccB1I GGYRCC 1 cut(s) 675
AccB7I CCANNNNNTGG 1 cut(s) 670
AccII CGCG 2 cut(s) 1998, 2091
AciI CCGC 5 cut(s) 48, 807, 907, 1327, 2089
AclWI GGATC 5 cut(s) 38, 51, 682, 2497, 2501
AcoI YGGCCR 3 cut(s) 1215, 1286, 1343
AcsI RAATTY 8 cut(s) 79, 268, 477, 591, 1437, 1588, 1682, 1805
AcyI GRCGYC 2 cut(s) 740, 1728
AfaI GTAC 9 cut(s) 57, 249, 1139, 1171, 1952, 2123, 2297, 2842, 2879
AfeI AGCGCT 1 cut(s) 2714
AfiI CCNNNNNNNGG 5 cut(s) 469, 670, 1716, 2095, 2866
AflII CTTAAG 2 cut(s) 1637, 1676
AflIII ACRYGT 3 cut(s) 394, 1996, 2594
AhlI ACTAGT 2 cut(s) 1532, 2566
AjnI CCWGG 2 cut(s) 1319, 2341
AjuI GAANNNNNNNTTGG 2 cut(s) 986, 1018
Alw21I GWGCWC 2 cut(s) 406, 1123
Alw26I GTCTC 6 cut(s) 68, 731, 818, 920, 2352, 2422
Alw44I GTGCAC 1 cut(s) 402
AlwI GGATC 5 cut(s) 38, 51, 682, 2497, 2501
Ama87I CYCGRG 1 cut(s) 580
Aor51HI AGCGCT 1 cut(s) 2714
AoxI GGCC 3 cut(s) 1215, 1286, 1343
ApaLI GTGCAC 1 cut(s) 402
ApeKI GCWGC 6 cut(s) 146, 833, 836, 1014, 1295, 1925
ApoI RAATTY 8 cut(s) 79, 268, 477, 591, 1437, 1588, 1682, 1805
Asp700I GAANNNNTTC 2 cut(s) 477, 728
AspLEI GCGC 3 cut(s) 1507, 2715, 2815
AspS9I GGNCC 1 cut(s) 2585
AsuHPI GGTGA 4 cut(s) 631, 1256, 2342, 2407
AsuII TTCGAA 1 cut(s) 1207
AvaI CYCGRG 1 cut(s) 580
AvaII GGWCC 1 cut(s) 2585
BaeGI GKGCMC 2 cut(s) 406, 2861
BalI TGGCCA 3 cut(s) 1217, 1288, 1345
BamHI GGATCC 1 cut(s) 43
BanI GGYRCC 1 cut(s) 675
BarI GAAGNNNNNNTAC 2 cut(s) 2105, 2137
BbsI GAAGAC 3 cut(s) 225, 2142, 2703
Bbv12I GWGCWC 2 cut(s) 406, 1123
BbvCI CCTCAGC 1 cut(s) 954
BbvI GCAGC 6 cut(s) 133, 823, 845, 1001, 1307, 1912
BccI CCATC 7 cut(s) 379, 413, 1241, 1561, 1765, 2021, 2419
BceAI ACGGC 1 cut(s) 945
BcgI CGANNNNNNTGC 2 cut(s) 2366, 2400
BciT130I CCWGG 2 cut(s) 1321, 2343
BciVI GTATCC 2 cut(s) 463, 1358
BclI TGATCA 3 cut(s) 331, 430, 438
BcoDI GTCTC 6 cut(s) 68, 731, 818, 920, 2352, 2422
BcuI ACTAGT 2 cut(s) 1532, 2566
BfaI CTAG 8 cut(s) 111, 1152, 1277, 1533, 1628, 2117, 2567, 2721
BfmI CTRYAG 2 cut(s) 1330, 2749
BfoI RGCGCY 2 cut(s) 2716, 2816
BfrI CTTAAG 2 cut(s) 1637, 1676
BfuI GTATCC 2 cut(s) 463, 1358
BglII AGATCT 2 cut(s) 1051, 2803
BisI GCNGC 6 cut(s) 147, 834, 837, 1015, 1296, 1926
BlpI GCTNAGC 1 cut(s) 503
BlsI GCNGC 6 cut(s) 148, 835, 838, 1016, 1297, 1927
BmcAI AGTACT 2 cut(s) 249, 1952
Bme1390I CCNGG 2 cut(s) 1321, 2343
Bme18I GGWCC 1 cut(s) 2585
BmeT110I CYCGRG 1 cut(s) 580
BmgT120I GGNCC 1 cut(s) 2585
BmiI GGNNCC 4 cut(s) 45, 677, 1325, 2210
BmrFI CCNGG 2 cut(s) 1321, 2343
BmrI ACTGGG 1 cut(s) 591
BmsI GCATC 9 cut(s) 528, 979, 989, 1172, 1342, 1831, 1912, 1995, 2299
BmuI ACTGGG 1 cut(s) 591
BoxI GACNNNNGTC 1 cut(s) 386
BpiI GAAGAC 3 cut(s) 225, 2142, 2703
BpmI CTGGAG 2 cut(s) 819, 2879
Bpu10I CCTNAGC 2 cut(s) 954, 1508
Bpu1102I GCTNAGC 1 cut(s) 503
Bpu14I TTCGAA 1 cut(s) 1207
BpuEI CTTGAG 3 cut(s) 1667, 2345, 2545
BsaAI YACGTR 3 cut(s) 2008, 2595, 2881
BsaHI GRCGYC 2 cut(s) 740, 1728
BsaI GGTCTC 2 cut(s) 818, 2352
BsaJI CCNNGG 4 cut(s) 300, 346, 2167, 2342
BsaWI WCCGGW 2 cut(s) 263, 2074
Bsc4I CCNNNNNNNGG 5 cut(s) 469, 670, 1716, 2095, 2866
Bse1I ACTGG 7 cut(s) 136, 597, 1710, 1738, 1885, 2264, 2896
Bse3DI GCAATG 2 cut(s) 661, 1224
BseBI CCWGG 2 cut(s) 1321, 2343
BseDI CCNNGG 4 cut(s) 300, 346, 2167, 2342
BseGI GGATG 8 cut(s) 310, 543, 559, 1187, 1197, 1473, 1575, 1846
BseLI CCNNNNNNNGG 5 cut(s) 469, 670, 1716, 2095, 2866
BseMI GCAATG 2 cut(s) 661, 1224
BseMII CTCAG 3 cut(s) 494, 945, 2844
BseNI ACTGG 7 cut(s) 136, 597, 1710, 1738, 1885, 2264, 2896
BseRI GAGGAG 1 cut(s) 1295
BseSI GKGCMC 2 cut(s) 406, 2861
BseXI GCAGC 6 cut(s) 133, 823, 845, 1001, 1307, 1912
BseYI CCCAGC 1 cut(s) 1335
BsgI GTGCAG 2 cut(s) 1781, 2812
Bsh1236I CGCG 2 cut(s) 1998, 2091
BshFI GGCC 3 cut(s) 1217, 1288, 1345
BshNI GGYRCC 1 cut(s) 675
BsiHKAI GWGCWC 2 cut(s) 406, 1123
BsiHKCI CYCGRG 1 cut(s) 580
BsiSI CCGG 3 cut(s) 264, 2075, 2810
BslFI GGGAC 1 cut(s) 1985
BslI CCNNNNNNNGG 5 cut(s) 469, 670, 1716, 2095, 2866
BsmAI GTCTC 6 cut(s) 68, 731, 818, 920, 2352, 2422
BsmBI CGTCTC 2 cut(s) 731, 920
BsmFI GGGAC 1 cut(s) 1985
BsmI GAATGC 3 cut(s) 181, 809, 2404
BsnI GGCC 3 cut(s) 1217, 1288, 1345
Bso31I GGTCTC 2 cut(s) 818, 2352
BsoBI CYCGRG 1 cut(s) 580
Bsp119I TTCGAA 1 cut(s) 1207
Bsp1286I GDGCHC 3 cut(s) 406, 1123, 2861
Bsp1720I GCTNAGC 1 cut(s) 503
Bsp19I CCATGG 2 cut(s) 300, 2167
BspACI CCGC 5 cut(s) 48, 807, 907, 1327, 2089
BspANI GGCC 3 cut(s) 1217, 1288, 1345
BspCNI CTCAG 3 cut(s) 495, 946, 2845
BspFNI CGCG 2 cut(s) 1998, 2091
BspHI TCATGA 3 cut(s) 757, 1357, 1383
BspLI GGNNCC 4 cut(s) 45, 677, 1325, 2210
BspPI GGATC 5 cut(s) 38, 51, 682, 2497, 2501
BspQI GCTCTTC 1 cut(s) 82
BspT104I TTCGAA 1 cut(s) 1207
BspT107I GGYRCC 1 cut(s) 675
BspTI CTTAAG 2 cut(s) 1637, 1676
BspTNI GGTCTC 2 cut(s) 818, 2352
BsrDI GCAATG 2 cut(s) 661, 1224
BsrI ACTGG 7 cut(s) 136, 597, 1710, 1738, 1885, 2264, 2896
BssECI CCNNGG 4 cut(s) 300, 346, 2167, 2342
BssNI GRCGYC 2 cut(s) 740, 1728
BssT1I CCWWGG 3 cut(s) 300, 346, 2167
Bst2UI CCWGG 2 cut(s) 1321, 2343
Bst4CI ACNGT 5 cut(s) 343, 769, 1169, 2213, 2665
Bst6I CTCTTC 3 cut(s) 82, 2277, 2663
BstACI GRCGYC 2 cut(s) 740, 1728
BstAFI CTTAAG 2 cut(s) 1637, 1676
BstAPI GCANNNNNTGC 1 cut(s) 660
BstBAI YACGTR 3 cut(s) 2008, 2595, 2881
BstBI TTCGAA 1 cut(s) 1207
BstC8I GCNNGC 5 cut(s) 297, 1930, 1934, 2357, 2746
BstDEI CTNAG 9 cut(s) 99, 411, 503, 954, 1508, 1523, 1655, 1715, 2853
BstDSI CCRYGG 2 cut(s) 300, 2167
BstF5I GGATG 8 cut(s) 310, 543, 559, 1187, 1197, 1473, 1575, 1846
BstFNI CGCG 2 cut(s) 1998, 2091
BstH2I RGCGCY 2 cut(s) 2716, 2816
BstHHI GCGC 3 cut(s) 1507, 2715, 2815
BstMAI GTCTC 6 cut(s) 68, 731, 818, 920, 2352, 2422
BstMWI GCNNNNNNNGC 7 cut(s) 660, 830, 1402, 2597, 2721, 2760, 3001
BstNI CCWGG 2 cut(s) 1321, 2343
BstNSI RCATGY 2 cut(s) 398, 1932
BstPAI GACNNNNGTC 1 cut(s) 386
BstSCI CCNGG 2 cut(s) 1319, 2341
BstSFI CTRYAG 2 cut(s) 1330, 2749
BstSLI GKGCMC 2 cut(s) 406, 2861
BstUI CGCG 2 cut(s) 1998, 2091
BstV1I GCAGC 6 cut(s) 133, 823, 845, 1001, 1307, 1912
BstV2I GAAGAC 3 cut(s) 225, 2142, 2703
BstX2I RGATCY 5 cut(s) 43, 687, 1051, 2489, 2803
BstYI RGATCY 5 cut(s) 43, 687, 1051, 2489, 2803
BsuI GTATCC 2 cut(s) 463, 1358
BsuRI GGCC 3 cut(s) 1217, 1288, 1345
BtgI CCRYGG 2 cut(s) 300, 2167
BtsCI GGATG 8 cut(s) 310, 543, 559, 1187, 1197, 1473, 1575, 1846
BtsI GCAGTG 1 cut(s) 1544
BtsIMutI CAGTG 6 cut(s) 143, 339, 1544, 1731, 2183, 2829
Cac8I GCNNGC 5 cut(s) 297, 1930, 1934, 2357, 2746
CciI TCATGA 3 cut(s) 757, 1357, 1383
CfoI GCGC 3 cut(s) 1507, 2715, 2815
Cfr13I GGNCC 1 cut(s) 2585
CseI GACGC 2 cut(s) 1987, 2004
Csp6I GTAC 9 cut(s) 56, 248, 1138, 1170, 1951, 2122, 2296, 2841, 2878
CviQI GTAC 9 cut(s) 56, 248, 1138, 1170, 1951, 2122, 2296, 2841, 2878
DdeI CTNAG 9 cut(s) 99, 411, 503, 954, 1508, 1523, 1655, 1715, 2853
DraI TTTAAA 2 cut(s) 153, 1896
EaeI YGGCCR 3 cut(s) 1215, 1286, 1343
Eam1104I CTCTTC 3 cut(s) 82, 2277, 2663
EarI CTCTTC 3 cut(s) 82, 2277, 2663
Eco130I CCWWGG 3 cut(s) 300, 346, 2167
Eco31I GGTCTC 2 cut(s) 818, 2352
Eco32I GATATC 4 cut(s) 62, 1039, 1742, 1770
Eco47I GGWCC 1 cut(s) 2585
Eco47III AGCGCT 1 cut(s) 2714
Eco88I CYCGRG 1 cut(s) 580
EcoRI GAATTC 1 cut(s) 591
EcoRII CCWGG 2 cut(s) 1319, 2341
EcoRV GATATC 4 cut(s) 62, 1039, 1742, 1770
EcoT14I CCWWGG 3 cut(s) 300, 346, 2167
EcoT22I ATGCAT 2 cut(s) 1187, 1988
ErhI CCWWGG 3 cut(s) 300, 346, 2167
Esp3I CGTCTC 2 cut(s) 731, 920
FalI AAGNNNNNCTT 6 cut(s) 408, 440, 986, 1018, 2507, 2539
FaqI GGGAC 1 cut(s) 1985
FauI CCCGC 1 cut(s) 55
FauNDI CATATG 1 cut(s) 2576
FbaI TGATCA 3 cut(s) 331, 430, 438
Fnu4HI GCNGC 6 cut(s) 147, 834, 837, 1015, 1296, 1926
FokI GGATG 8 cut(s) 317, 550, 566, 1194, 1204, 1460, 1562, 1853
Fsp4HI GCNGC 6 cut(s) 147, 834, 837, 1015, 1296, 1926
FspBI CTAG 8 cut(s) 111, 1152, 1277, 1533, 1628, 2117, 2567, 2721
GlaI GCGC 3 cut(s) 1506, 2714, 2814
GluI GCNGC 6 cut(s) 147, 834, 837, 1015, 1296, 1926
GsaI CCCAGC 1 cut(s) 1339
GsuI CTGGAG 2 cut(s) 819, 2879
HaeII RGCGCY 2 cut(s) 2716, 2816
HaeIII GGCC 3 cut(s) 1217, 1288, 1345
HapII CCGG 3 cut(s) 264, 2075, 2810
HgaI GACGC 2 cut(s) 1987, 2004
HhaI GCGC 3 cut(s) 1507, 2715, 2815
Hin1I GRCGYC 2 cut(s) 740, 1728
Hin6I GCGC 3 cut(s) 1505, 2713, 2813
HinP1I GCGC 3 cut(s) 1505, 2713, 2813
HincII GTYRAC 2 cut(s) 943, 1878
HindII GTYRAC 2 cut(s) 943, 1878
HindIII AAGCTT 2 cut(s) 1146, 1403
HinfI GANTC 8 cut(s) 497, 568, 716, 761, 1651, 2047, 2611, 2733
HpaII CCGG 3 cut(s) 264, 2075, 2810
HphI GGTGA 4 cut(s) 631, 1256, 2342, 2407
Hpy166II GTNNAC 8 cut(s) 404, 943, 1274, 1878, 1912, 2124, 2203, 2941
Hpy188I TCNGA 9 cut(s) 198, 709, 949, 985, 1237, 1471, 1689, 1921, 2494
Hpy8I GTNNAC 8 cut(s) 404, 943, 1274, 1878, 1912, 2124, 2203, 2941
Hpy99I CGWCG 3 cut(s) 202, 385, 745
HpyAV CCTTC 8 cut(s) 310, 505, 1171, 1235, 1355, 2462, 2492, 2871
HpyCH4III ACNGT 5 cut(s) 343, 769, 1169, 2213, 2665
HpyCH4IV ACGT 6 cut(s) 740, 1728, 2007, 2594, 2780, 2880
HpyF10VI GCNNNNNNNGC 7 cut(s) 660, 830, 1402, 2597, 2721, 2760, 3001
HpyF3I CTNAG 9 cut(s) 99, 411, 503, 954, 1508, 1523, 1655, 1715, 2853
HpySE526I ACGT 6 cut(s) 740, 1728, 2007, 2594, 2780, 2880
Hsp92I GRCGYC 2 cut(s) 740, 1728
HspAI GCGC 3 cut(s) 1505, 2713, 2813
Ksp22I TGATCA 3 cut(s) 331, 430, 438
LguI GCTCTTC 1 cut(s) 82
LmnI GCTCC 2 cut(s) 1329, 1437
Lsp1109I GCAGC 6 cut(s) 133, 823, 845, 1001, 1307, 1912
LweI GCATC 9 cut(s) 528, 979, 989, 1172, 1342, 1831, 1912, 1995, 2299
MaeI CTAG 8 cut(s) 111, 1152, 1277, 1533, 1628, 2117, 2567, 2721
MaeII ACGT 6 cut(s) 740, 1728, 2007, 2594, 2780, 2880
MaeIII GTNAC 5 cut(s) 337, 599, 1446, 1613, 1729
MfeI CAATTG 2 cut(s) 846, 1044
MflI RGATCY 5 cut(s) 43, 687, 1051, 2489, 2803
MhlI GDGCHC 3 cut(s) 406, 1123, 2861
MlsI TGGCCA 3 cut(s) 1217, 1288, 1345
MluI ACGCGT 1 cut(s) 1996
MluNI TGGCCA 3 cut(s) 1217, 1288, 1345
MlyI GAGTC 3 cut(s) 577, 2041, 2742
MmeI TCCRAC 6 cut(s) 21, 221, 352, 1704, 1869, 2673
Mox20I TGGCCA 3 cut(s) 1217, 1288, 1345
Mph1103I ATGCAT 2 cut(s) 1187, 1988
MroXI GAANNNNTTC 2 cut(s) 477, 728
MscI TGGCCA 3 cut(s) 1217, 1288, 1345
MseI TTAA 8 cut(s) 152, 323, 1610, 1638, 1677, 1895, 2616, 2769
MslI CAYNNNNRTG 3 cut(s) 399, 1190, 2946
Msp20I TGGCCA 3 cut(s) 1217, 1288, 1345
MspA1I CMGCKG 3 cut(s) 50, 836, 1335
MspCI CTTAAG 2 cut(s) 1637, 1676
MspI CCGG 3 cut(s) 264, 2075, 2810
MspR9I CCNGG 2 cut(s) 1321, 2343
MunI CAATTG 2 cut(s) 846, 1044
Mva1269I GAATGC 3 cut(s) 181, 809, 2404
MvaI CCWGG 2 cut(s) 1321, 2343
MvnI CGCG 2 cut(s) 1998, 2091
MwoI GCNNNNNNNGC 7 cut(s) 660, 830, 1402, 2597, 2721, 2760, 3001
NcoI CCATGG 2 cut(s) 300, 2167
NdeI CATATG 1 cut(s) 2576
NlaIV GGNNCC 4 cut(s) 45, 677, 1325, 2210
NmuCI GTSAC 3 cut(s) 337, 599, 1729
NsiI ATGCAT 2 cut(s) 1187, 1988
NspI RCATGY 2 cut(s) 398, 1932
NspV TTCGAA 1 cut(s) 1207
PaeI GCATGC 1 cut(s) 1932
PaeR7I CTCGAG 1 cut(s) 580
PagI TCATGA 3 cut(s) 757, 1357, 1383
PciI ACATGT 1 cut(s) 394
PciSI GCTCTTC 1 cut(s) 82
PctI GAATGC 3 cut(s) 181, 809, 2404
PdmI GAANNNNTTC 2 cut(s) 477, 728
PfeI GAWTC 5 cut(s) 497, 716, 761, 1651, 2611
PflFI GACNNNGTC 1 cut(s) 2732
PflMI CCANNNNNTGG 1 cut(s) 670
PkrI GCNGC 6 cut(s) 148, 835, 838, 1016, 1297, 1927
PleI GAGTC 3 cut(s) 576, 2041, 2741
PpsI GAGTC 3 cut(s) 576, 2041, 2741
Ppu21I YACGTR 3 cut(s) 2008, 2595, 2881
PscI ACATGT 1 cut(s) 394
PshAI GACNNNNGTC 1 cut(s) 386
PsiI TTATAA 2 cut(s) 36, 545
Psp6I CCWGG 2 cut(s) 1319, 2341
PspFI CCCAGC 1 cut(s) 1335
PspGI CCWGG 2 cut(s) 1319, 2341
PspN4I GGNNCC 4 cut(s) 45, 677, 1325, 2210
PspPI GGNCC 1 cut(s) 2585
PsuI RGATCY 5 cut(s) 43, 687, 1051, 2489, 2803
PsyI GACNNNGTC 1 cut(s) 2732
PvuII CAGCTG 2 cut(s) 836, 1335
RsaI GTAC 9 cut(s) 57, 249, 1139, 1171, 1952, 2123, 2297, 2842, 2879
RsaNI GTAC 9 cut(s) 56, 248, 1138, 1170, 1951, 2122, 2296, 2841, 2878
RseI CAYNNNNRTG 3 cut(s) 399, 1190, 2946
SapI GCTCTTC 1 cut(s) 82
SaqAI TTAA 8 cut(s) 152, 323, 1610, 1638, 1677, 1895, 2616, 2769
SatI GCNGC 6 cut(s) 147, 834, 837, 1015, 1296, 1926
Sau96I GGNCC 1 cut(s) 2585
ScaI AGTACT 2 cut(s) 249, 1952
SchI GAGTC 3 cut(s) 577, 2041, 2742
ScrFI CCNGG 2 cut(s) 1321, 2343
SduI GDGCHC 3 cut(s) 406, 1123, 2861
SfaNI GCATC 9 cut(s) 528, 979, 989, 1172, 1342, 1831, 1912, 1995, 2299
SfcI CTRYAG 2 cut(s) 1330, 2749
Sfr274I CTCGAG 1 cut(s) 580
SfuI TTCGAA 1 cut(s) 1207
SinI GGWCC 1 cut(s) 2585
SlaI CTCGAG 1 cut(s) 580
SmiMI CAYNNNNRTG 3 cut(s) 399, 1190, 2946
SmlI CTYRAG 6 cut(s) 580, 1637, 1646, 1676, 2360, 2524
SmoI CTYRAG 6 cut(s) 580, 1637, 1646, 1676, 2360, 2524
SpeI ACTAGT 2 cut(s) 1532, 2566
SphI GCATGC 1 cut(s) 1932
SsiI CCGC 5 cut(s) 48, 807, 907, 1327, 2089
SspI AATATT 2 cut(s) 860, 1415
SspMI CTAG 8 cut(s) 111, 1152, 1277, 1533, 1628, 2117, 2567, 2721
StyD4I CCNGG 2 cut(s) 1319, 2341
StyI CCWWGG 3 cut(s) 300, 346, 2167
TaaI ACNGT 5 cut(s) 343, 769, 1169, 2213, 2665
TaiI ACGT 6 cut(s) 743, 1731, 2010, 2597, 2783, 2883
TaqI TCGA 4 cut(s) 581, 1207, 1772, 2922
TatI WGTACW 4 cut(s) 247, 1950, 2121, 2295
TfiI GAWTC 5 cut(s) 497, 716, 761, 1651, 2611
Tru1I TTAA 8 cut(s) 152, 323, 1610, 1638, 1677, 1895, 2616, 2769
Tru9I TTAA 8 cut(s) 152, 323, 1610, 1638, 1677, 1895, 2616, 2769
TscAI CASTG 6 cut(s) 143, 346, 1551, 1738, 2190, 2836
TseFI GTSAC 3 cut(s) 337, 599, 1729
TseI GCWGC 6 cut(s) 146, 833, 836, 1014, 1295, 1925
Tsp45I GTSAC 3 cut(s) 337, 599, 1729
TspGWI ACGGA 2 cut(s) 215, 1612
TspRI CASTG 6 cut(s) 143, 346, 1551, 1738, 2190, 2836
Tth111I GACNNNGTC 1 cut(s) 2732
Van91I CCANNNNNTGG 1 cut(s) 670
Vha464I CTTAAG 2 cut(s) 1637, 1676
VneI GTGCAC 1 cut(s) 402
VpaK11BI GGWCC 1 cut(s) 2585
XapI RAATTY 8 cut(s) 79, 268, 477, 591, 1437, 1588, 1682, 1805
XbaI TCTAGA 1 cut(s) 1151
XceI RCATGY 2 cut(s) 398, 1932
XcmI CCANNNNNNNNNTGG 1 cut(s) 1296
XhoI CTCGAG 1 cut(s) 580
XmnI GAANNNNTTC 2 cut(s) 477, 728
XspI CTAG 8 cut(s) 111, 1152, 1277, 1533, 1628, 2117, 2567, 2721
ZraI GACGTC 2 cut(s) 741, 1729
ZrmI AGTACT 2 cut(s) 249, 1952
Zsp2I ATGCAT 2 cut(s) 1187, 1988
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.