Rroxscaffold_6G00408070

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000085
Physical Location & Seq
Reverse (-)
30580293 .. 30591141
10849 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00408070.1

Sequence Viewer

Length: 2646 bp
ATGGTGAAATACGTTGGAGTACAAGCCCAAGAAAGTAGGCTTTTTAATCAAGTGATTATGGCTGTTGTATCCCAAAACCCCGACTTGATGAAAATTCAACAGACATTTGCAGAAATGCTGGGCTTTAAATTGGAGGAGAAGACAGAAATTGGAAGAGCCATGAAATTGAAGGAGAAGATAATGAGAGTAACTGGGATCCTCATAATCTTGGATAACATTTGGAAGAGAATAGACTTTTCAGACATTGGAATTCCGAGCCATAACGAACTTCAAAGACGCAATTCCAAAATTTTACTGACCACCAGAAAATTGAGTGTTTGCCGTTCCATGGATTGCCATGAAAATGTACATCTCAATATCTTATCAGTAGAAGATTCTTGGAGCTTATTTGGGAAGGAAGCAAGGAAGTCTTTCCACAAATCATCCAATTTCTATGATGTAGCACGTAAGGTGGCTAGAGAATGTGCTGGTCTACCACTTGCTTTGAGAGCAGTTGCGAGGGCCCTTCGAGATGAAGGTTTAGATGGATGGAAAGAAGCTGCTCGACGACTACAAGCGTCCCAACCTACCAACCCTGAAGATGAAGATGAGAAAGATGTGTTTACATGCATAAAGTTAAGCTATGATTACTTGAAATCTGATGATTCCAAATCATGCTTCTTGCTTTGCTGCCTGTTCCCAGAAGATTATGATATCCCAATTGAATACTTGCTCCAGTATGGAATTGGGAAAGGAATGTTTCAAGATTCCAACATGCTAGAAGCCCGAGCCACAACATATTCAGTGGTGAAGGCCCTTAAAGATTCTAGCTTGCTTTTGGACGCTAGATATGACGGATGTGTAAGGATGCATGATGTCATTCGGGATATGGCAATATTAATTTCATTATCTGTAGACGGCCAGCGGTTCTTGGTCAAAGTTGGCTGTGAATTGAAGAATTGGCCAAAGATTGATGCACATAAAGGCTACTCTGCAATCTCACTAATGAAGAACAAAATTTGCAAGCTACCCGAAGAGTTGGTATGTTCAAATCTCCAGATTTTATTACTGCAAGACAATGCTTCATTAAATGATATCCCTGATTCTTTCTTCCAAAATCAGAAAGAACTAAGAGTCTTGGATCTTAGCCGCACTGGTATTTCATTACTACCCCAATCAATCAGTTTCCTAAGCAACCTTCAAGCTTTGTATTTAGATTACTGCAAGAACATAATTGACATTTCTGTAATCGGAAAACTTAACAAGCTTGAGATTCTTAGTATGAGAGGAAATGCTCTGAAAGAATTGCCAAGAGAAATAGGACGGTTGACCAGTCTAAGGATGCTGGATGTCACTAGAGCTGATAGATTCCTTTTCACATCCAGGAGTGGATGTATTGGCACAATTCCATCTAAAGTGATATCAAAGTTGCATAAATTAGAAGAACTGTACATGCAATGTGGATTTTGGGACTGGGGGAGTAAAATTGCTGGAGAAGGAGAAGAAACGAATATTGGCTTTGATGAATTAGCTGGTTTATCATATTTAAGCATTTTGAAAGTTTGGATATCAGATGCAAATTGCATCCCTATAAGTGTTGAGGTCGAACCGAATTGGGTTTACTTTGATATTACTATTTGCAGCAACAGTTATTTCACTTCGAACGGTCAATCTGGTCATAATTGTAGATCCTTGAGTTTTAATGGAACTGACACTACCATAGGAATCTGTCCGGACTGGTTTGTCAACGCTGTGGTAAAGAATACTGAGAGGCTACTTTATAGTTACTGCGAAGGGTTAAGTAACATTCTTGTGGAATATGACCGTGGAAGGTTACATGGATTGAAAGTTCTCTCTGTAAAGGGTCGCTGTGAGAACTTGAAAGAATTGATGAATGCAATAACATGTGTTCTAGATGTGCCTGTGTTTGAGAATTTGGAAGAGTTGTATCTGGAATACCTGGATTACCTGAAGCAGTTATGCGTTGGTGAGTTACCACCAGGATCTCTCTGCAGTCTGAAATTATTGAAGGTCCTTAAGTGTCCTATCTTGGGGAATGTACTGTTGCCATCTAAATTGTTGCAGCAACTACCAACTCTGGAAAAGCTAATCTGTGAGTCAAGTGAAGTGGAACATGTGTTTGGATGTGAAGGATTTGAGCCAGATCAAACAAATCTGAGAGAGATAGATTTACGGTATCTAGATGTCGTAAGAAGCATATGTAATGGTCCTGCTCCACATGGAATGTTCCAGGCTCTAAAGAAATTGGTCATTCGTAATTGCAACTTCGAGGGAAGCCTCTTCACATTTGATGTAGCTCAGTGTCTTTTTCAATTGGAAGACCTTATGGTATCTGAATGCCCTGTCTTGGAAAGAGTAATTGAAGAAAGGAGGGAAACATTGAACAACAAGAAGACCATTCTTCCAATATTGAAGAACCTACTTTTGGAAGATCTTCCAATGTTGTACAAGGGAAGTGCTACTATTGATTTTGAGTGTCCTTCATTGGAAAAATTGAGTCTGTTTGGCTGCCCCCACTTTTCATTTCCATCCTCTGCTTCTGACTACTTCCACAGCAGGAACCAAGTTGTTTTCAATGATGCTCCTCGTCATTCTCTGCTACTCAGCAAAATGGGGTATAAGGGATTCAAAATATCAAGTTGGTAG

Protein Analysis

881

Amino Acids

99.92

Weight (kDa)

6.0

Isoelectric Point (pI)

40.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 12 - 135 4.1e-17 NB-ARC domain
LRR_14 PF23598 328 - 483 8.1e-12 Leucine-rich repeat region
LRR_8 PF13855 343 - 401 1.8e-09 Leucine rich repeat
LRR_RPS2 PF23247 639 - 780 1.2e-18 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 721 - 838 4.3e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1721
AccI GTMKAC 2 cut(s) 472, 894
AccIII TCCGGA 1 cut(s) 1711
AciI CCGC 2 cut(s) 904, 1129
AclWI GGATC 5 cut(s) 190, 203, 1128, 1662, 1990
AcoI YGGCCR 2 cut(s) 898, 941
AcsI RAATTY 5 cut(s) 93, 249, 288, 996, 1912
AcuI CTGAAG 2 cut(s) 597, 1970
AfaI GTAC 5 cut(s) 21, 348, 1430, 2040, 2447
AfiI CCNNNNNNNGG 5 cut(s) 328, 1317, 2030, 2347, 2425
AflII CTTAAG 1 cut(s) 2015
AflIII ACRYGT 2 cut(s) 1883, 2113
AjnI CCWGG 4 cut(s) 1361, 1938, 1978, 2229
AjuI GAANNNNNNNTTGG 8 cut(s) 641, 673, 1476, 1508, 2103, 2135, 2408, 2440
AloI GAACNNNNNNTCC 2 cut(s) 1812, 1844
AlwI GGATC 5 cut(s) 190, 203, 1128, 1662, 1990
Ama87I CYCGRG 1 cut(s) 765
Aor13HI TCCGGA 1 cut(s) 1711
AoxI GGCC 4 cut(s) 501, 792, 898, 941
ApaI GGGCCC 1 cut(s) 505
ApeKI GCWGC 5 cut(s) 539, 669, 1620, 2062, 2508
ApoI RAATTY 5 cut(s) 93, 249, 288, 996, 1912
AseI ATTAAT 1 cut(s) 878
Asp700I GAANNNNTTC 2 cut(s) 410, 2433
AspS9I GGNCC 5 cut(s) 501, 502, 793, 2011, 2207
AsuHPI GGTGA 3 cut(s) 16, 799, 1979
AsuII TTCGAA 1 cut(s) 1640
AvaI CYCGRG 1 cut(s) 765
AvaII GGWCC 2 cut(s) 2011, 2207
BaeGI GKGCMC 1 cut(s) 505
BalI TGGCCA 1 cut(s) 943
BamHI GGATCC 1 cut(s) 195
BanII GRGCYC 1 cut(s) 505
BbsI GAAGAC 3 cut(s) 146, 2325, 2399
BbvI GCAGC 5 cut(s) 526, 656, 1632, 2074, 2495
BccI CCATC 5 cut(s) 518, 522, 1396, 2056, 2536
BceAI ACGGC 2 cut(s) 306, 913
BciT130I CCWGG 4 cut(s) 1363, 1940, 1980, 2231
BciVI GTATCC 1 cut(s) 79
BfaI CTAG 7 cut(s) 456, 758, 807, 825, 1335, 1892, 2180
BfmI CTRYAG 2 cut(s) 891, 1990
BfrI CTTAAG 1 cut(s) 2015
BfuI GTATCC 1 cut(s) 79
BglII AGATCT 1 cut(s) 2431
BisI GCNGC 6 cut(s) 540, 670, 1129, 1621, 2063, 2509
BlsI GCNGC 6 cut(s) 541, 671, 1130, 1622, 2064, 2510
Bme1390I CCNGG 4 cut(s) 1363, 1940, 1980, 2231
Bme18I GGWCC 2 cut(s) 2011, 2207
BmeT110I CYCGRG 1 cut(s) 765
BmgT120I GGNCC 5 cut(s) 501, 502, 793, 2011, 2207
BmiI GGNNCC 3 cut(s) 197, 503, 2561
BmrFI CCNGG 4 cut(s) 1363, 1940, 1980, 2231
BmrI ACTGGG 2 cut(s) 201, 1462
BmsI GCATC 6 cut(s) 837, 943, 1311, 1543, 1572, 2569
BmuI ACTGGG 2 cut(s) 201, 1462
BpiI GAAGAC 3 cut(s) 146, 2325, 2399
BpmI CTGGAG 3 cut(s) 698, 1019, 1491
Bpu10I CCTNAGC 1 cut(s) 1169
Bpu14I TTCGAA 1 cut(s) 1640
BpuEI CTTGAG 2 cut(s) 1268, 1693
BsaAI YACGTR 1 cut(s) 446
BsaJI CCNNGG 2 cut(s) 327, 1804
BsaWI WCCGGW 1 cut(s) 1711
BsaXI ACNNNNNCTCC 2 cut(s) 1357, 1387
Bsc4I CCNNNNNNNGG 5 cut(s) 328, 1317, 2030, 2347, 2425
Bse1I ACTGG 6 cut(s) 196, 715, 1138, 1311, 1457, 1721
Bse3DI GCAATG 1 cut(s) 1442
BseAI TCCGGA 1 cut(s) 1711
BseBI CCWGG 4 cut(s) 1363, 1940, 1980, 2231
BseDI CCNNGG 2 cut(s) 327, 1804
BseLI CCNNNNNNNGG 5 cut(s) 328, 1317, 2030, 2347, 2425
BseMI GCAATG 1 cut(s) 1442
BseMII CTCAG 4 cut(s) 1737, 2147, 2312, 2617
BseNI ACTGG 6 cut(s) 196, 715, 1138, 1311, 1457, 1721
BseRI GAGGAG 2 cut(s) 149, 2574
BseSI GKGCMC 1 cut(s) 505
BseXI GCAGC 5 cut(s) 526, 656, 1632, 2074, 2495
BseYI CCCAGC 1 cut(s) 118
BshFI GGCC 4 cut(s) 503, 794, 900, 943
BsiHKCI CYCGRG 1 cut(s) 765
BsiSI CCGG 1 cut(s) 1712
BslFI GGGAC 2 cut(s) 544, 1463
BslI CCNNNNNNNGG 5 cut(s) 328, 1317, 2030, 2347, 2425
BsmFI GGGAC 2 cut(s) 544, 1463
BsmI GAATGC 2 cut(s) 1879, 2342
BsnI GGCC 4 cut(s) 503, 794, 900, 943
BsoBI CYCGRG 1 cut(s) 765
Bsp119I TTCGAA 1 cut(s) 1640
Bsp120I GGGCCC 1 cut(s) 501
Bsp1286I GDGCHC 1 cut(s) 505
Bsp13I TCCGGA 1 cut(s) 1711
Bsp1407I TGTACA 3 cut(s) 346, 1428, 2445
Bsp143I GATC 6 cut(s) 195, 1120, 1667, 1982, 2143, 2431
Bsp19I CCATGG 1 cut(s) 327
BspACI CCGC 2 cut(s) 904, 1129
BspANI GGCC 4 cut(s) 503, 794, 900, 943
BspCNI CTCAG 4 cut(s) 1738, 2148, 2311, 2616
BspEI TCCGGA 1 cut(s) 1711
BspLI GGNNCC 3 cut(s) 197, 503, 2561
BspMAI CTGCAG 1 cut(s) 1994
BspPI GGATC 5 cut(s) 190, 203, 1128, 1662, 1990
BspQI GCTCTTC 1 cut(s) 148
BspT104I TTCGAA 1 cut(s) 1640
BspTI CTTAAG 1 cut(s) 2015
BsrDI GCAATG 1 cut(s) 1442
BsrGI TGTACA 3 cut(s) 346, 1428, 2445
BsrI ACTGG 6 cut(s) 196, 715, 1138, 1311, 1457, 1721
BssECI CCNNGG 2 cut(s) 327, 1804
BssMI GATC 6 cut(s) 195, 1120, 1667, 1982, 2143, 2431
BssT1I CCWWGG 1 cut(s) 327
Bst2UI CCWGG 4 cut(s) 1363, 1940, 1980, 2231
Bst4CI ACNGT 7 cut(s) 1305, 1428, 1628, 1646, 1805, 2043, 2175
Bst6I CTCTTC 5 cut(s) 148, 218, 1008, 1914, 2285
BstAFI CTTAAG 1 cut(s) 2015
BstAUI TGTACA 3 cut(s) 346, 1428, 2445
BstBAI YACGTR 1 cut(s) 446
BstBI TTCGAA 1 cut(s) 1640
BstC8I GCNNGC 3 cut(s) 812, 902, 1004
BstDEI CTNAG 9 cut(s) 1109, 1124, 1169, 1256, 1316, 1746, 2156, 2298, 2603
BstDSI CCRYGG 2 cut(s) 327, 1804
BstKTI GATC 6 cut(s) 198, 1123, 1670, 1985, 2146, 2434
BstMBI GATC 6 cut(s) 195, 1120, 1667, 1982, 2143, 2431
BstMWI GCNNNNNNNGC 1 cut(s) 488
BstNI CCWGG 4 cut(s) 1363, 1940, 1980, 2231
BstNSI RCATGY 5 cut(s) 609, 757, 1435, 1887, 2117
BstSCI CCNGG 4 cut(s) 1361, 1938, 1978, 2229
BstSFI CTRYAG 2 cut(s) 891, 1990
BstSLI GKGCMC 1 cut(s) 505
BstV1I GCAGC 5 cut(s) 526, 656, 1632, 2074, 2495
BstV2I GAAGAC 3 cut(s) 146, 2325, 2399
BstX2I RGATCY 5 cut(s) 195, 1120, 1667, 1982, 2431
BstYI RGATCY 5 cut(s) 195, 1120, 1667, 1982, 2431
BsuI GTATCC 1 cut(s) 79
BsuRI GGCC 4 cut(s) 503, 794, 900, 943
BtgI CCRYGG 2 cut(s) 327, 1804
BtsIMutI CAGTG 3 cut(s) 789, 1131, 2306
Cac8I GCNNGC 3 cut(s) 812, 902, 1004
Cfr13I GGNCC 5 cut(s) 501, 502, 793, 2011, 2207
CseI GACGC 3 cut(s) 285, 546, 830
Csp6I GTAC 5 cut(s) 20, 347, 1429, 2039, 2446
CspCI CAANNNNNGTGG 4 cut(s) 465, 500, 2088, 2123
CviQI GTAC 5 cut(s) 20, 347, 1429, 2039, 2446
DdeI CTNAG 9 cut(s) 1109, 1124, 1169, 1256, 1316, 1746, 2156, 2298, 2603
DpnI GATC 6 cut(s) 197, 1122, 1669, 1984, 2145, 2433
DpnII GATC 6 cut(s) 195, 1120, 1667, 1982, 2143, 2431
DraI TTTAAA 1 cut(s) 127
DrdI GACNNNNNNGTC 1 cut(s) 1721
DseDI GACNNNNNNGTC 1 cut(s) 1721
EaeI YGGCCR 2 cut(s) 898, 941
Eam1104I CTCTTC 5 cut(s) 148, 218, 1008, 1914, 2285
EarI CTCTTC 5 cut(s) 148, 218, 1008, 1914, 2285
Eco130I CCWWGG 1 cut(s) 327
Eco24I GRGCYC 1 cut(s) 505
Eco32I GATATC 4 cut(s) 694, 1075, 1401, 1548
Eco47I GGWCC 2 cut(s) 2011, 2207
Eco57I CTGAAG 2 cut(s) 597, 1970
Eco88I CYCGRG 1 cut(s) 765
EcoO109I RGGNCCY 4 cut(s) 501, 502, 793, 2011
EcoRI GAATTC 1 cut(s) 249
EcoRII CCWGG 4 cut(s) 1361, 1938, 1978, 2229
EcoRV GATATC 4 cut(s) 694, 1075, 1401, 1548
EcoT14I CCWWGG 1 cut(s) 327
EcoT22I ATGCAT 2 cut(s) 611, 852
EcoT38I GRGCYC 1 cut(s) 505
ErhI CCWWGG 1 cut(s) 327
FalI AAGNNNNNCTT 2 cut(s) 394, 426
FaqI GGGAC 2 cut(s) 544, 1463
FauNDI CATATG 1 cut(s) 2198
FblI GTMKAC 2 cut(s) 472, 894
Fnu4HI GCNGC 6 cut(s) 540, 670, 1129, 1621, 2063, 2509
FriOI GRGCYC 1 cut(s) 505
Fsp4HI GCNGC 6 cut(s) 540, 670, 1129, 1621, 2063, 2509
FspBI CTAG 7 cut(s) 456, 758, 807, 825, 1335, 1892, 2180
GluI GCNGC 6 cut(s) 540, 670, 1129, 1621, 2063, 2509
GsaI CCCAGC 1 cut(s) 122
GsuI CTGGAG 3 cut(s) 698, 1019, 1491
HaeIII GGCC 4 cut(s) 503, 794, 900, 943
HapII CCGG 1 cut(s) 1712
HgaI GACGC 3 cut(s) 285, 546, 830
HincII GTYRAC 2 cut(s) 1308, 1726
HindII GTYRAC 2 cut(s) 1308, 1726
HindIII AAGCTT 2 cut(s) 1182, 1244
HpaII CCGG 1 cut(s) 1712
HphI GGTGA 3 cut(s) 16, 799, 1979
Hpy166II GTNNAC 6 cut(s) 473, 603, 895, 1308, 1600, 1726
Hpy188III TCNNGA 9 cut(s) 509, 743, 863, 1036, 1712, 1892, 1931, 2078, 2180
Hpy8I GTNNAC 6 cut(s) 473, 603, 895, 1308, 1600, 1726
Hpy99I CGWCG 1 cut(s) 549
HpyCH4III ACNGT 7 cut(s) 1305, 1428, 1628, 1646, 1805, 2043, 2175
HpyCH4IV ACGT 2 cut(s) 12, 445
HpyF10VI GCNNNNNNNGC 1 cut(s) 488
HpyF3I CTNAG 9 cut(s) 1109, 1124, 1169, 1256, 1316, 1746, 2156, 2298, 2603
HpySE526I ACGT 2 cut(s) 12, 445
Kpn2I TCCGGA 1 cut(s) 1711
Kzo9I GATC 6 cut(s) 195, 1120, 1667, 1982, 2143, 2431
LguI GCTCTTC 1 cut(s) 148
LmnI GCTCC 4 cut(s) 381, 717, 2218, 2587
Lsp1109I GCAGC 5 cut(s) 526, 656, 1632, 2074, 2495
LweI GCATC 6 cut(s) 837, 943, 1311, 1543, 1572, 2569
MaeI CTAG 7 cut(s) 456, 758, 807, 825, 1335, 1892, 2180
MaeII ACGT 2 cut(s) 12, 445
MaeIII GTNAC 6 cut(s) 187, 1330, 1763, 1781, 1812, 1971
MalI GATC 6 cut(s) 197, 1122, 1669, 1984, 2145, 2433
MboI GATC 6 cut(s) 195, 1120, 1667, 1982, 2143, 2431
MfeI CAATTG 2 cut(s) 699, 2312
MflI RGATCY 5 cut(s) 195, 1120, 1667, 1982, 2431
MhlI GDGCHC 1 cut(s) 505
MlsI TGGCCA 1 cut(s) 943
MluNI TGGCCA 1 cut(s) 943
MlyI GAGTC 3 cut(s) 1122, 2105, 2506
MmeI TCCRAC 1 cut(s) 774
Mox20I TGGCCA 1 cut(s) 943
Mph1103I ATGCAT 2 cut(s) 611, 852
MroI TCCGGA 1 cut(s) 1711
MroXI GAANNNNTTC 2 cut(s) 410, 2433
MscI TGGCCA 1 cut(s) 943
MslI CAYNNNNRTG 2 cut(s) 342, 1790
Msp20I TGGCCA 1 cut(s) 943
MspA1I CMGCKG 1 cut(s) 904
MspCI CTTAAG 1 cut(s) 2015
MspI CCGG 1 cut(s) 1712
MspR9I CCNGG 4 cut(s) 1363, 1940, 1980, 2231
MunI CAATTG 2 cut(s) 699, 2312
Mva1269I GAATGC 2 cut(s) 1879, 2342
MvaI CCWGG 4 cut(s) 1363, 1940, 1980, 2231
MwoI GCNNNNNNNGC 1 cut(s) 488
NcoI CCATGG 1 cut(s) 327
NdeI CATATG 1 cut(s) 2198
NdeII GATC 6 cut(s) 195, 1120, 1667, 1982, 2143, 2431
NlaIV GGNNCC 3 cut(s) 197, 503, 2561
NmuCI GTSAC 1 cut(s) 1330
NsiI ATGCAT 2 cut(s) 611, 852
NspI RCATGY 5 cut(s) 609, 757, 1435, 1887, 2117
NspV TTCGAA 1 cut(s) 1640
PciI ACATGT 2 cut(s) 1883, 2113
PciSI GCTCTTC 1 cut(s) 148
PctI GAATGC 2 cut(s) 1879, 2342
PdmI GAANNNNTTC 2 cut(s) 410, 2433
PfeI GAWTC 9 cut(s) 374, 644, 746, 803, 1082, 1252, 1347, 1704, 2625
PfoI TCCNGGA 1 cut(s) 1361
PkrI GCNGC 6 cut(s) 541, 671, 1130, 1622, 2064, 2510
PleI GAGTC 3 cut(s) 1121, 2104, 2505
PpsI GAGTC 3 cut(s) 1121, 2104, 2505
Ppu21I YACGTR 1 cut(s) 446
PpuMI RGGWCCY 1 cut(s) 2011
PscI ACATGT 2 cut(s) 1883, 2113
PshBI ATTAAT 1 cut(s) 878
Psp5II RGGWCCY 1 cut(s) 2011
Psp6I CCWGG 4 cut(s) 1361, 1938, 1978, 2229
PspFI CCCAGC 1 cut(s) 118
PspGI CCWGG 4 cut(s) 1361, 1938, 1978, 2229
PspN4I GGNNCC 3 cut(s) 197, 503, 2561
PspOMI GGGCCC 1 cut(s) 501
PspPI GGNCC 5 cut(s) 501, 502, 793, 2011, 2207
PspPPI RGGWCCY 1 cut(s) 2011
PsrI GAACNNNNNNTAC 2 cut(s) 1678, 1710
PstI CTGCAG 1 cut(s) 1994
PsuI RGATCY 5 cut(s) 195, 1120, 1667, 1982, 2431
RsaI GTAC 5 cut(s) 21, 348, 1430, 2040, 2447
RsaNI GTAC 5 cut(s) 20, 347, 1429, 2039, 2446
RseI CAYNNNNRTG 2 cut(s) 342, 1790
SapI GCTCTTC 1 cut(s) 148
SatI GCNGC 6 cut(s) 540, 670, 1129, 1621, 2063, 2509
Sau3AI GATC 6 cut(s) 195, 1120, 1667, 1982, 2143, 2431
Sau96I GGNCC 5 cut(s) 501, 502, 793, 2011, 2207
SchI GAGTC 3 cut(s) 1122, 2105, 2506
ScrFI CCNGG 4 cut(s) 1363, 1940, 1980, 2231
SduI GDGCHC 1 cut(s) 505
SfaNI GCATC 6 cut(s) 837, 943, 1311, 1543, 1572, 2569
SfcI CTRYAG 2 cut(s) 891, 1990
SfuI TTCGAA 1 cut(s) 1640
SinI GGWCC 2 cut(s) 2011, 2207
SmiMI CAYNNNNRTG 2 cut(s) 342, 1790
SmlI CTYRAG 3 cut(s) 1247, 1672, 2015
SmoI CTYRAG 3 cut(s) 1247, 1672, 2015
SsiI CCGC 2 cut(s) 904, 1129
SspI AATATT 3 cut(s) 876, 1492, 2409
SspMI CTAG 7 cut(s) 456, 758, 807, 825, 1335, 1892, 2180
StyD4I CCNGG 4 cut(s) 1361, 1938, 1978, 2229
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 7 cut(s) 1305, 1428, 1628, 1646, 1805, 2043, 2175
TaiI ACGT 2 cut(s) 15, 448
TaqI TCGA 5 cut(s) 508, 544, 1584, 1640, 2268
TatI WGTACW 5 cut(s) 19, 346, 1428, 2038, 2445
TauI GCSGC 1 cut(s) 1131
TfiI GAWTC 9 cut(s) 374, 644, 746, 803, 1082, 1252, 1347, 1704, 2625
TscAI CASTG 3 cut(s) 789, 1138, 2306
TseFI GTSAC 1 cut(s) 1330
TseI GCWGC 5 cut(s) 539, 669, 1620, 2062, 2508
Tsp45I GTSAC 1 cut(s) 1330
TspGWI ACGGA 1 cut(s) 849
TspRI CASTG 3 cut(s) 789, 1138, 2306
Vha464I CTTAAG 1 cut(s) 2015
VpaK11BI GGWCC 2 cut(s) 2011, 2207
VspI ATTAAT 1 cut(s) 878
XapI RAATTY 5 cut(s) 93, 249, 288, 996, 1912
XbaI TCTAGA 2 cut(s) 1891, 2179
XceI RCATGY 5 cut(s) 609, 757, 1435, 1887, 2117
XcmI CCANNNNNNNNNTGG 1 cut(s) 722
XmiI GTMKAC 2 cut(s) 472, 894
XmnI GAANNNNTTC 2 cut(s) 410, 2433
XspI CTAG 7 cut(s) 456, 758, 807, 825, 1335, 1892, 2180
Zsp2I ATGCAT 2 cut(s) 611, 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.